Cell factory carrier suitable for automatic cell culture
By designing cell factory vehicles suitable for automated cell culture, the pollution problems caused by slow diffusion of carbon dioxide gas and manual operation in traditional technology are solved, and rapid gas-circuit docking and fully automated operation are achieved, improving the efficiency and stability of cell culture.
Patent Information
- Application Number
- CN202421302702.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-07
- Publication Date
- 2025-03-25
- Estimated Expiration
- 2034-06-07
AI Technical Summary
In the existing cell culture technology, traditional breathable covers lead to slow diffusion and permeation of carbon dioxide gas, affecting the quality of cell growth; at the same time, manual operation leads to external pollution and an unstable culture environment, limiting production efficiency and scale.
Design a cell factory vehicle suitable for automated cell culture, including vehicle body, cell factory fixed structure, gas sealed docking structure and clamping structure to achieve powerless gas path docking and fully automated operation.
By quickly connecting and disconnecting the gas circuit and sealing the gas circuit, the addition of power units in the incubator is avoided, the risk of contamination is reduced, and the robot is fully automated, which improves the efficiency and stability of cell culture.
Smart Images
Figure CN222665860U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture equipment, in particular to a cell factory carrier suitable for automated cell culture. Background Art
[0002] In the existing cell culture process, the cell factory uses a traditional breathable cover to obtain carbon dioxide gas through the diffusion penetration cover of the traditional incubator cavity. However, the diffusion and penetration speed of carbon dioxide gas in this way is relatively slow, and it takes 8 hours or even longer to make the carbon dioxide gas concentration in each layer of the 10-layer cell factory reach the required concentration. Such a delay affects the growth quality of cells and may lead to an unstable culture environment and inconsistent growth effects.
[0003] Moreover, the current cell culture relies on manually taking the cell factory out of a place and then placing it in a traditional incubator for culture. However, this method will bring external pollution and an unstable culture environment, affecting the normal growth and culture effect of cells; relying on manual operation is not suitable for large-scale production and automated production processes, which will increase labor costs and limit production efficiency and scale.
[0004] Therefore, it is necessary to design a new vehicle to achieve unpowered gas circuit docking, so that the carbon dioxide gas circuit in the cell factory can be quickly connected and disconnected, and the gas circuit can be sealed to avoid adding additional power devices in the incubator and polluting the culture environment; and it can be suitable for fully automated operation of the robot without human participation, reducing the potential contamination risks caused by manual operation. Utility Model Content
[0005] The purpose of the utility model is to overcome the defects of the prior art and provide a cell factory carrier suitable for automated cell culture.
[0006] In order to solve the above technical problems, the purpose of the utility model is achieved through the following technical solutions: providing a cell factory carrier suitable for automated cell culture, comprising: a carrier body, a cell factory fixing structure, a gas-sealed docking structure and a clamping structure; the cell factory fixing structure, the gas-sealed docking structure and the clamping structure are respectively installed on the carrier body, and the clamping structure is connected to a manipulator.
[0007] A further technical solution is as follows: a guide roller is installed below the carrier, and the guide roller is in rolling contact with a guide rail in the incubator.
[0008] A further technical solution is as follows: the cell factory fixing structure comprises a limit mounting plate, a knob spring pin, and a locking knob; the knob spring pin and the locking knob are respectively connected to the limit mounting plate.
[0009] A further technical solution is as follows: the cell factory fixing structure comprises a locking position adjustment plate, the locking position adjustment plate is connected to the carrier body, and the locking knob is connected to the locking position adjustment plate.
[0010] A further technical solution is as follows: a groove is provided in the locking position adjustment plate, and one end of the locking knob is embedded in the groove.
[0011] A further technical solution is as follows: the clamping structure comprises a clamping plate, and the clamping plate is installed on the outer side of the carrier body.
[0012] Its further technical solution is: the gas sealing docking structure includes an air guide plate, an air pipe limiting seat and a connecting pipe, the connecting pipe is connected to the air pipe limiting seat, the air pipe limiting seat is provided with a through groove, the air guide plate is provided with a cavity, and the connecting pipe, the through groove and the cavity are connected.
[0013] Its further technical solution is: a through hole is provided on the air guide plate, the through hole is communicated with the cavity, the through hole is connected with a pagoda air pipe connector, and the pagoda air pipe connector is connected to the cell factory through a hose.
[0014] A further technical solution is: the connecting pipe includes a silicone hose.
[0015] A further technical solution is: the cell factory is connected to a sterile filter, and the sterile filter is connected to the hose.
[0016] Compared with the prior art, the utility model has the following beneficial effects: the utility model provides a carrier body, a cell factory fixing structure, a gas-sealed docking structure and a clamping structure; the cell factory fixing structure can fix and remove the cell factory; the gas-sealed docking structure can quickly dock, disconnect and seal the gas path; the clamping structure is matched with a manipulator to clamp and move the carrier body, thereby achieving fully automated operation and reducing manual intervention; the entire carrier achieves unpowered gas path docking, so that the carbon dioxide gas path in the cell factory can be quickly connected and disconnected, and the gas path can be sealed, thus avoiding the need for an additional power device in the incubator to pollute the culture environment; and the utility model is suitable for fully automated operation of the manipulator without manual intervention, thereby reducing the potential risk of pollution caused by manual operation.
[0017] The utility model is further described below in conjunction with the accompanying drawings and specific embodiments. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0019] Figure 1 A schematic diagram of a three-dimensional structure of a cell factory carrier suitable for automated cell culture provided in an embodiment of the utility model Figure 1 ;
[0020] Figure 2 A schematic diagram of a three-dimensional structure of a cell factory carrier suitable for automated cell culture provided in an embodiment of the utility model Figure 2 ;
[0021] Figure 3 A schematic diagram of a three-dimensional structure of a cell factory carrier suitable for automated cell culture and a cell factory connected thereto provided in an embodiment of the utility model;
[0022] Description of the symbols in the figure:
[0023] 1. Carrier body; 2. Locking knob; 3. Locking position adjustment plate; 4. Knob spring pin; 5. Roller; 6. Pagoda air pipe connector; 7. Air pipe limit seat; 8. Connecting pipe; 9. Air guide plate; 10. Limit mounting plate; 11. Clamping plate; 12. Cell factory; 13. Hose; 14. Sterile filter. DETAILED DESCRIPTION
[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0025] It should be understood that when used in this specification and the appended claims, the terms "include" and "comprises" indicate the presence of described features, integers, steps, operations, elements and / or components, but do not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components and / or combinations thereof.
[0026] It should also be understood that the terms used in this utility model specification are only for the purpose of describing specific embodiments and are not intended to limit the utility model. As used in this utility model specification and the appended claims, unless the context clearly indicates otherwise, the singular forms "a", "an" and "the" are intended to include plural forms.
[0027] It should be further understood that the term “and / or” used in the present specification and the appended claims refers to any combination and all possible combinations of one or more of the associated listed items, and includes these combinations.
[0028] See also Figure 1 , Figure 1 A schematic diagram of a three-dimensional structure of a cell factory carrier suitable for automated cell culture provided in an embodiment of the utility model Figure 1 It can be used in the process of cell culture to achieve unpowered gas circuit docking, so that the carbon dioxide gas circuit in the cell factory 12 can be quickly connected and disconnected, and the gas circuit can be sealed, avoiding the addition of additional power devices in the incubator to pollute the culture environment; and it can be suitable for fully automated operation of the robot without manual participation, reducing the potential pollution risks caused by manual operation.
[0029] See also Figure 1 A cell factory carrier suitable for automated cell culture includes: a carrier body 1, a cell factory 12 fixing structure, a gas-sealed docking structure and a clamping structure; the cell factory 12 fixing structure, the gas-sealed docking structure and the clamping structure are respectively installed on the carrier body 1, and the clamping structure is connected to a manipulator.
[0030] In this embodiment, the carrier body 1 is a five-sided hollow bracket with a supporting surface on the bottom. The cell factory 12 is placed from the top of the carrier body 1, which is conducive to sufficient heat exchange of the cell factory 12 in the incubator and ensures that the temperature of each layer of cells remains consistent.
[0031] In one embodiment, see Figure 1 A guide roller 5 is installed below the above-mentioned carrier, and the guide roller 5 is in rolling contact with the guide rail in the incubator.
[0032] In this embodiment, there are four guide rollers 5. Of course, the number of guide rollers 5 can be set according to actual conditions. The setting of the guide rollers can cooperate with the guide rails in the incubator to easily move the carrier into the incubator and accurately connect the gas sealing docking structure with the air pipe joint in the incubator.
[0033] In one embodiment, see Figure 1 and Figure 3The above-mentioned cell factory 12 fixing structure includes a limit mounting plate 10, a knob spring pin 4, and a locking knob 2. The knob spring pin 4 and the locking knob 2 are respectively connected to the limit mounting plate 10.
[0034] In one embodiment, see Figure 1 and Figure 3 The above-mentioned cell factory 12 fixing structure includes a locking position adjustment plate 3 , the locking position adjustment plate 3 is connected to the carrier body 1 , and the locking knob 2 is connected to the locking position adjustment plate 3 .
[0035] In one embodiment, see Figure 1 and Figure 3 The locking position adjustment plate 3 is provided with a groove, and one end of the locking knob 2 is embedded in the groove. The fixing structure of the cell factory 12 can adjust the fixing position and is suitable for cell factories 12 with 1 to 10 layers.
[0036] Loosen the locking knob 2 and slide the locking knob 2 up and down in the groove of the locking position adjustment plate 3 to drive the limit mounting plate 10 to move up and down, so that the knob spring pin 4 presses the cell factory 12, and then tighten the locking knob 2 to fix the cell factory 12 in the carrier body 1. If the cell factory 12 is to be taken out, pull out the knob spring pin 4 and rotate the rotating spring pin to retract the rotating spring pin, thereby loosening the fixation of the cell factory 12 and taking out the cell factory 12 from the carrier body 1.
[0037] In one embodiment, see Figure 2 The above-mentioned clamping structure includes a clamping plate 11, which is installed on the outside of the carrier body 1 and connected to the manipulator.
[0038] The clamping plate 11 is arranged to cooperate with the manipulator for clamping by the manipulator, and the manipulator pushes the carrier body 1 to move along the guide rail in the incubator, so that the connecting tube 8 of the gas sealing docking structure is automatically plugged and connected with the gas connector in the incubator. After the cultivation is completed, the manipulator clamps the carrier body 1 and pulls it outward to pull out the connecting tube 8 and disconnect the gas circuit.
[0039] In one embodiment, see Figure 1 and Figure 3 The above-mentioned gas sealing docking structure includes an air guide plate 9, an air pipe limiting seat 7 and a connecting pipe 8. The connecting pipe 8 is connected to the air pipe limiting seat 7. The air pipe limiting seat 7 is provided with a through groove. The air guide plate 9 is provided with a cavity. The connecting pipe 8, the through groove and the cavity are connected.
[0040] In one embodiment, see Figure 1 and Figure 3The air guide plate 9 is provided with a through hole, which is communicated with the cavity, and the through hole is connected with a pagoda air pipe connector 6, which is connected with the cell factory 12 through a hose 13.
[0041] In one embodiment, see Figure 1 and Figure 3 The connecting pipe 8 mentioned above includes a silicone hose 13 .
[0042] In this embodiment, the silicone hose 13 is fixed in the air pipe limiting seat 7 and is connected to the pagoda air pipe connector 6 through the air guide plate 9.
[0043] In one embodiment, see Figure 3 The cell factory 12 is connected to a sterile filter 14 , and the sterile filter 14 is connected to a hose 13 .
[0044] Connect the ventilation hose 13 with a sterile filter 14 between the cover of the cell factory 12 and the pagoda air pipe connector 6. After the silicone hose 13 is connected to the air path in the incubator, the cell factory 12 can be connected and sealed with the air path in the incubator, thereby achieving the control of the concentration of carbon dioxide, oxygen and water vapor in the cell factory 12.
[0045] The carrier of this embodiment is designed with guide rollers 5, guide rails and gas-sealed docking structures. Under the push of the manipulator, the carrier body 1 moves along the guide rails in the incubator, and the connecting pipe 8 of the gas-sealed docking structure is automatically plugged and connected with the gas connector in the incubator. This design can reduce friction dust generation and enable the gas circuit to be quickly connected and disconnected without the intervention of an additional power device; the gas-sealed docking structure includes an air guide plate 9, a trachea limit seat 7 and a connecting pipe 8, and the connecting pipe 8 is connected to the trachea limit seat 7. During the connection process, these components cooperate with each other, and the design of the gas-sealed docking structure is used to achieve the sealing of the gas circuit, avoid gas leakage, and ensure the cleanliness of the culture environment. The carrier is designed with a clamping structure, and through the cooperation of the clamping plate 11 and the manipulator, the manipulator can automatically clamp the carrier body 1 and perform mobile operations, thereby realizing fully automated operation. In addition, the fixing structure of the cell factory 12 is also carefully designed, and the cell factory 12 can be easily taken out or fixed without manual intervention, thereby reducing the potential contamination risk caused by manual operation.
[0046] In general, the design realizes the functions of unpowered gas path docking, disconnection and sealing through reasonable coordination and structural design among various components, while adapting to the fully automated operation of the manipulator, thus ensuring the efficiency, safety and pollution-free nature of the cell culture process.
[0047] The above-mentioned cell factory carrier suitable for automated cell culture, by setting a carrier body 1, a cell factory 12 fixing structure, a gas-sealed docking structure and a clamping structure, the cell factory 12 fixing structure can realize the fixation and removal of the cell factory 12, the gas-sealed docking structure realizes the rapid docking, disconnection and sealing of the gas path, the clamping structure is matched with a manipulator, and the manipulator clamps and moves the carrier body 1, thereby realizing fully automated operation and reducing manual intervention. The entire carrier realizes unpowered gas path docking, so that the carbon dioxide gas path in the cell factory 12 can be quickly connected and disconnected, and the gas path can be sealed, avoiding the addition of an additional power device in the incubator to pollute the culture environment; and it can be suitable for fully automated operation of the manipulator, without the need for manual participation, reducing the potential pollution risks caused by manual operation.
[0048] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any technician familiar with the technical field can easily think of various equivalent modifications or replacements within the technical scope disclosed by the utility model, and these modifications or replacements should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.
Claims
1. A cell factory carrier suitable for automated cell culture, characterized in that: include: A carrier body, a cell factory fixing structure, a gas-sealed docking structure and a clamping structure; the cell factory fixing structure, the gas-sealed docking structure and the clamping structure are respectively installed on the carrier body, and the clamping structure is connected to a manipulator.
2. A cell factory carrier suitable for automated cell culture according to claim 1, characterized in that: A guide roller is installed below the carrier, and the guide roller is in rolling contact with the guide rail in the incubator.
3. A cell factory carrier suitable for automated cell culture according to claim 1, characterized in that: The cell factory fixing structure comprises a limit mounting plate, a knob spring pin, and a locking knob. The knob spring pin and the locking knob are respectively connected to the limit mounting plate.
4. A cell factory carrier suitable for automated cell culture according to claim 3, characterized in that: The cell factory fixing structure comprises a locking position adjustment plate, the locking position adjustment plate is connected to the carrier body, and the locking knob is connected to the locking position adjustment plate.
5. A cell factory carrier suitable for automated cell culture according to claim 4, characterized in that: A groove is provided in the locking position adjustment plate, and one end of the locking knob is embedded in the groove.
6. A cell factory carrier suitable for automated cell culture according to claim 1, characterized in that: The clamping structure comprises a clamping plate, and the clamping plate is installed on the outer side of the carrier body.
7. A cell factory carrier suitable for automated cell culture according to claim 1, characterized in that: The gas sealing docking structure includes an air guide plate, an air pipe limiting seat and a connecting pipe, the connecting pipe is connected to the air pipe limiting seat, a through groove is provided in the air pipe limiting seat, a cavity is provided in the air guide plate, and the connecting pipe, the through groove and the cavity are connected.
8. A cell factory carrier suitable for automated cell culture according to claim 7, characterized in that: The air guide plate is provided with a through hole, the through hole is communicated with the cavity, the through hole is connected with a pagoda air pipe joint, and the pagoda air pipe joint is connected with the cell factory through a hose.
9. A cell factory carrier suitable for automated cell culture according to claim 8, characterized in that: The connecting pipe comprises a silicone hose.
10. A cell factory carrier suitable for automated cell culture according to claim 8, characterized in that: The cell factory is connected to a sterile filter, and the sterile filter is connected to the hose.