Salmonella phage screening device

Abstract

The utility model discloses a salmonella bacteriophage screening device, relates to the technical field of salmonella bacteriophage screening, and aims to solve the problems that when multi-thallus culture is carried out on existing culture dishes, most of the culture dishes are directly placed in one culture dish, mixing is easily caused, observation is influenced, and screening is inaccurate. A culture cavity is formed in the culture dish body, the culture cavity and the culture dish body are of an integrated structure, and a protective cover is arranged at the upper end of the culture dish body; the central column is arranged in the culture cavity, partition plates are arranged on the peripheral surfaces of the central column, and the partition plates and the central column are of an integrated structure; the limiting plates are arranged on the inner wall of the culture cavity, the limiting plates are connected with the inner wall of the culture cavity in a hot melting mode, limiting inserting grooves are formed in the limiting plates, the three faces of each limiting inserting groove are open, the partition plates are inserted into the limiting inserting grooves, and the number of the partition plates and the number of the limiting plates are both four.

Description

Technical Field

[0001] The utility model relates to the technical field of Salmonella phage screening, in particular to a Salmonella phage screening device. Background Art

[0002] Salmonella is a common foodborne pathogen. The traditional method of identifying Salmonella is mainly based on morphological characteristics, culture characteristics, physiological and biochemical characteristics, antigenic characteristics, phage characteristics, etc. During screening, the enrichment solution is added to the culture dish for observation to achieve screening;

[0003] For example, the Chinese authorized patent (culture dish) with announcement number CN218811716U: includes a culture dish body, the outside of the culture dish body is provided with an outer sheath, the inside of the culture dish body is provided with a first partition plate, one side of the bottom end of the culture dish body is provided with a fixed block, one side of the fixed block is provided with a support leg laterally, and the bottom end of the fixed block is provided with an inner groove. During the use of the culture dish, when it is necessary to heat the inside of the culture dish, the three groups of support legs at the bottom of the culture dish body can be hinged between the connector and the inside of the inner groove, and flipped down to be perpendicular to the bottom of the culture dish body. Then, the three groups of support legs can be used to prop up the culture dish body on the table, and then the bottom of the culture dish can be directly heated with an alcohol lamp at the bottom of the culture dish body. No bracket is needed, so it is convenient and quick to use, and solves the problem of inconvenience in providing auxiliary support for the culture dish.

[0004] For example, the Chinese patent with publication number CN105861451A (a method for rapid screening of virulent bacteriophages) includes sample collection and phage acquisition, activation of test bacteria and preparation of single colonies, and then cross-streaking inoculation of bacterial single colonies and phages to screen out phages that can lyse test strains.

[0005] However, when conducting multi-bacteria culture, most of the existing culture dishes are placed directly inside one culture dish, which easily causes mixing, thereby affecting observation and causing inaccurate screening; therefore, it does not meet existing needs. In this regard, we propose a Salmonella phage screening device. Utility Model Content

[0006] The utility model aims to provide a Salmonella phage screening device to solve the problem that most of the existing culture dishes mentioned in the above background technology are directly placed inside one culture dish when culturing multiple bacteria, which easily causes mixing, thereby affecting observation and leading to inaccurate screening.

[0007] To achieve the above-mentioned object, the utility model provides the following technical solutions: a Salmonella phage screening device, comprising: a culture dish body, a culture cavity is arranged inside the culture dish body, and the culture cavity and the culture dish body are an integrated structure, and a protective cover is arranged on the upper end of the culture dish body;

[0008] Also includes:

[0009] A central column, which is arranged inside the culture chamber, and the surrounding surfaces of the central column are provided with partition plates, and the partition plates and the central column are an integrated structure;

[0010] A limiting plate is arranged on the inner wall of the culture chamber, and the limiting plate is hot-melt connected to the inner wall of the culture chamber. A limiting slot is arranged inside the limiting plate, and the limiting slot is open on three sides. The partition plate is inserted into the limiting slot. Four of the partition plates and the limiting plates are arranged.

[0011] Preferably, a positioning column is provided at the center of the bottom surface of the culture chamber, and the positioning column and the culture dish body are an integrated structure, and a positioning groove is provided on the lower surface of the center column, and the positioning column is inserted into the positioning groove.

[0012] Preferably, a connection hole is provided at the center of the top surface inside the protective cover, and a connection column is provided on the upper surface of the center column, and the connection column is inserted into the connection hole.

[0013] Preferably, a splicing hole is provided on the lower surface of the culture dish body, and the splicing hole and the culture dish body are an integral structure, and a splicing column is provided on the upper end of the protective cover.

[0014] Preferably, the surface of the splicing column is provided with an external thread, and the external thread and the splicing column are an integrated structure, the upper surface of the protective cover is provided with a thread groove, and the splicing column is threadably connected to the thread groove through the external thread.

[0015] Preferably, a partition cavity is provided between one side of the partition plate and the culture cavity, and four partition cavities are provided.

[0016] Compared with the prior art, the beneficial effects of the utility model are:

[0017] 1. The utility model arranges a central column with a partition plate inside the culture chamber, inserts the central column into the culture chamber, and aligns the positioning groove with the positioning column during insertion, so as to position the central column installation. At the same time, the partition plate is inserted into the limiting slot from top to bottom to fix the central column and the partition plate. The culture chamber is divided into four separation chambers by the partition plate, so that four bacteria can be observed separately without interfering with each other, which is convenient for observation and improves the accuracy of observation. The utility model solves the problem that most of the existing culture dishes are directly placed in one culture dish when culturing multiple bacteria, which is easy to cause mixing, thereby affecting observation and causing inaccurate screening.

[0018] 2. By setting splicing holes on the lower surface of the culture dish body and setting splicing columns on the upper end of the protective cover, the splicing columns are threadedly connected to the thread groove through external threads. After fixing the four splicing columns, when stacking the culture dishes, the splicing holes of the upper culture dish body are aligned with the splicing columns below, which has a limiting effect on the stacking of two culture dishes, avoids deviation and falling, and improves stability. The detachable splicing columns can be added according to needs to improve flexibility. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0020] Figure 2 It is a schematic diagram of the overall structure of the utility model when viewed from above;

[0021] Figure 3 This is a schematic diagram of the connection structure between the culture dish body and the central column of the utility model;

[0022] Figure 4 This is a schematic diagram of the central column structure of the utility model when viewed from above;

[0023] Figure 5 This is a schematic diagram of the internal structure of the culture dish of the utility model;

[0024] In the figure: 1. culture dish body; 2. protective cover; 3. culture chamber; 4. center column; 5. partition plate; 6. limit plate; 7. limit slot; 8. splicing column; 9. splicing hole; 10. connecting hole; 11. connecting column; 12. partition chamber; 13. positioning groove; 14. positioning column; 15. thread groove; 16. external thread. DETAILED DESCRIPTION

[0025] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0026] See also Figure 1-5The utility model provides an embodiment: a Salmonella phage screening device, comprising: a culture dish body 1, a culture cavity 3 is arranged inside the culture dish body 1, and the culture cavity 3 and the culture dish body 1 are an integrated structure, and a protective cover 2 is arranged on the upper end of the culture dish body 1;

[0027] Also includes:

[0028] A central column 4 is arranged inside the culture chamber 3, and partition plates 5 are arranged on all four sides of the central column 4, and the partition plates 5 and the central column 4 are an integral structure;

[0029] The limiting plate 6 is arranged on the inner wall of the culture chamber 3, and the limiting plate 6 is hot-melt connected to the inner wall of the culture chamber 3. A limiting slot 7 is arranged inside the limiting plate 6, and the limiting slot 7 is open on three sides. The partition plate 5 is inserted into the limiting slot 7. Four partition plates 5 and four limiting plates 6 are arranged.

[0030] The central column 4 is inserted into the culture chamber 3. When inserting, the positioning groove 13 is aligned with the positioning column 14, which has a positioning effect on the installation of the central column 4. At the same time, the partition plate 5 is inserted into the limiting slot 7 from top to bottom to fix the central column 4 and the partition plate 5. The culture chamber 3 is divided by the partition plate 5 into four separation chambers 12, so that the four bacteria can be separated and observed without interfering with each other, which facilitates observation and improves the accuracy of observation.

[0031] See also Figure 3 , 4 5. A positioning column 14 is provided at the center of the bottom surface of the culture chamber 3, and the positioning column 14 and the culture dish body 1 are an integral structure. A positioning groove 13 is provided on the lower surface of the center column 4, and the positioning column 14 is inserted into the positioning groove 13 to position the center column 4 and improve stability.

[0032] See also Figure 2 , 3 A connecting hole 10 is provided at the center of the top surface inside the protective cover 2 , a connecting column 11 is provided on the upper surface of the center column 4 , and the connecting column 11 is inserted into the connecting hole 10 , so as to further improve the stability of the center column 4 .

[0033] See also Figure 1 , 2 The lower surface of the culture dish body 1 is provided with a splicing hole 9, and the splicing hole 9 and the culture dish body 1 are an integral structure. The upper end of the protective cover 2 is provided with a splicing column 8. When stacking the culture dishes, the splicing hole 9 of the upper culture dish body 1 is aligned with the splicing column 8 below, which has a limiting effect on the stacking of the two culture dishes, avoids deviation and falling, and improves stability.

[0034] See also Figure 1 , 3The surface of the splicing column 8 is provided with an external thread 16, and the external thread 16 and the splicing column 8 are an integrated structure. The upper surface of the protective cover 2 is provided with a thread groove 15, and the splicing column 8 is threadedly connected with the thread groove 15 through the external thread 16, which is convenient for disassembly of the splicing column 8 and can be added according to needs to improve flexibility. A partition chamber 12 is provided between one side of the partition plate 5 and the culture chamber 3, and four partition chambers 12 are provided. The culture chamber 3 is divided into four partition chambers 12 by the partition plate 5, so that four bacteria can be separated and observed without interfering with each other, which is convenient for observation and improves the accuracy of observation.

[0035] Working principle: When in use, the center column 4 is inserted into the culture chamber 3. When inserting, the positioning groove 13 is aligned with the positioning column 14, which has a positioning effect on the installation of the center column 4. At the same time, the partition plate 5 is inserted into the limiting slot 7 from top to bottom to fix the center column 4 and the partition plate 5. The culture chamber 3 is divided by the partition plate 5 into four separation chambers 12, so that the four bacteria can be separated and observed without interfering with each other, which is convenient for observation and improves the accuracy of observation. The protective cover 2 is covered, and the connecting column 11 is inserted into the connecting hole 10 to further stabilize the center column 4. The splicing column 8 is threadedly connected to the thread groove 15 through the external thread 16. After the four splicing columns 8 are fixed, when stacking the culture dishes, the splicing hole 9 of the upper culture dish body 1 is aligned with the splicing column 8 below, which has a limiting effect on the stacking of the two culture dishes, avoids deviation and falling, and improves stability.

[0036] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

Claims

1. A Salmonella phage screening device, comprising a culture dish body (1), wherein a culture cavity (3) is arranged inside the culture dish body (1), and the culture cavity (3) and the culture dish body (1) are an integral structure, and a protective cover (2) is arranged at the upper end of the culture dish body (1); Features: Also includes: A central column (4) is arranged inside the culture chamber (3), and partition plates (5) are arranged on all four sides of the central column (4), and the partition plates (5) and the central column (4) are an integral structure; A limiting plate (6) is arranged on the inner wall of the culture chamber (3), and the limiting plate (6) is connected to the inner wall of the culture chamber (3) by hot melt. A limiting slot (7) is arranged inside the limiting plate (6), and the limiting slot (7) is open on three sides. The partition plate (5) is inserted into the limiting slot (7). Four of each of the partition plates (5) and the limiting plate (6) are provided.

2. The Salmonella phage screening device according to claim 1, characterized in that: A positioning column (14) is arranged at the center of the bottom surface of the culture chamber (3), and the positioning column (14) and the culture dish body (1) are an integral structure. A positioning groove (13) is arranged on the lower surface of the center column (4), and the positioning column (14) is inserted into the positioning groove (13).

3. The Salmonella phage screening device according to claim 1, characterized in that: A connection hole (10) is provided at the center of the top surface inside the protective cover (2), and a connection column (11) is provided on the upper surface of the center column (4), and the connection column (11) is inserted into the connection hole (10).

4. The Salmonella phage screening device according to claim 1, characterized in that: The lower surface of the culture dish body (1) is provided with a splicing hole (9), and the splicing hole (9) and the culture dish body (1) are an integral structure, and the upper end of the protective cover (2) is provided with a splicing column (8).

5. The Salmonella phage screening device according to claim 4, characterized in that: The surface of the splicing column (8) is provided with an external thread (16), and the external thread (16) and the splicing column (8) are an integral structure; the upper surface of the protective cover (2) is provided with a thread groove (15), and the splicing column (8) is threadedly connected to the thread groove (15) via the external thread (16).

6. The Salmonella phage screening device according to claim 1, characterized in that: A partition cavity (12) is provided between one side of the partition plate (5) and the culture cavity (3), and four partition cavities (12) are provided.

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