Mobile phase continuous liquid adding device for liquid chromatography

Abstract

A partition plate is arranged in a bottle cavity of the mobile phase continuous liquid adding device and divides the bottle cavity into a main cavity and a liquid supplementing cavity, the partition plate downwards extends to the position about one centimeter away from the bottom of the bottle, and the main cavity and the liquid supplementing cavity are communicated at the bottom of the bottle. A liquid supplementing opening leading to the liquid supplementing cavity is formed in the bottle cap, and a liquid supplementing channel is arranged between the bottle cap and the liquid supplementing cavity. And the supplemented mobile phase flows through the liquid supplementing channel, enters the liquid supplementing cavity and is further supplemented into the main cavity. The supplemented mobile phase and the mobile phase in the liquid supplementing cavity are mixed to generate bubbles, and the bubbles only float upwards in the liquid supplementing cavity, are blocked by the partition plate and cannot flow into the main cavity, so that the mobile phase in the main cavity is not influenced by the bubbles, cannot bring noise to the liquid chromatograph and cannot cause sharp noise peaks of an obtained chromatogram, and the quality of the liquid chromatograph is improved. And the analysis sensitivity of the liquid chromatograph is not reduced. The floating plug is arranged in the liquid supplementing cavity, and the floating plug floats upwards to plug the liquid supplementing channel along with supplementing of the mobile phase, so that the mobile phase in the liquid supplementing cavity is prevented from flowing back and overflowing, and the risk of leakage is avoided.

Description

Technical Field

[0001] The utility model relates to a mobile phase continuous liquid adding device for liquid chromatography. Background Art

[0002] As an essential reagent for liquid chromatography detection, the mobile phase needs to be prepared before use and loaded into the mobile phase adding device (mobile phase bottle) after preparation. The mobile phase adding device is provided with a bottle cap with a pipette opening. When the liquid chromatograph is running, its catheter extends into the mobile phase adding device to continuously and uniformly absorb the mobile phase into the instrument for use. The mobile phase needs to be replenished after it is consumed. Since the liquid chromatograph should not be paused during batch injection, when the instrument starts continuous injection detection, it is necessary to manually add the newly prepared mobile phase to the mobile phase adding device regularly during operation. For this reason, the operator needs to open the bottle cap for refilling while the instrument is running. During this process, the lower end of the catheter needs to be immersed in the remaining mobile phase. In this way, it is easy to generate bubbles when the bottle cap is opened and the liquid is poured in, and it is also easy to disturb the catheter. Bubbles entering the liquid chromatograph through the catheter will cause sharp noise peaks or decreased analytical sensitivity in the chromatogram obtained by the liquid chromatograph; when the bubbles become larger and enter the flow path or chromatographic column, the flow rate of the mobile phase will slow down or become unstable, causing the baseline to fluctuate and damage the instrument and chromatographic column. In addition, the mobile phase liquid is corrosive. If the liquid is added too full during replenishment, it will be dangerous if backflow and leakage occur. Utility Model Content

[0003] The utility model provides a mobile phase continuous liquid adding device for liquid chromatography, which can avoid the mobile phase in the bottle cavity from generating bubbles when adding liquid, and can avoid liquid backflow and leakage, thereby reducing the risk of backflow and leakage when filling.

[0004] A mobile phase continuous liquid addition device for liquid chromatography comprises a bottle cavity for containing mobile phase and a bottle cap covering the bottle mouth, characterized in that: the bottle cavity is divided by a partition to form a main cavity and a liquid replenishment cavity, a partition bottom end opening is provided at the bottom of the partition, the liquid replenishment cavity and the main cavity are connected at the partition bottom end opening, a straw opening leading to the main cavity and a liquid replenishment port leading to the liquid replenishment cavity are provided on the bottle cap, a liquid replenishment channel is provided between the liquid replenishment port and the liquid replenishment cavity, a floating plug is provided in the liquid replenishment cavity, and as the mobile phase is replenished, the floating plug floats up to plug the liquid replenishment channel.

[0005] Furthermore, a glass bottom-mouth bottle is provided, the lower part of which is provided with an opening, and an outlet pipe with a switch valve is provided on the outer side wall of the opening, and the mobile phase flows out from the outlet.

[0006] Furthermore, a hose is sleeved at the water outlet of the water outlet pipe with a switch valve, the hose extends into the fluid infusion channel, the outer wall of the hose sticks to the inner wall of the fluid infusion channel to form a closed pipeline, and the bottom end of the hose does not exceed the bottom end of the fluid infusion channel.

[0007] Furthermore, the bottle cap is provided with a partial flip cover at the liquid infusion port.

[0008] Furthermore, the fluid infusion channel is a glass cylinder.

[0009] Furthermore, the floating plug is spherical, and its diameter is greater than the height of the bottom opening of the partition.

[0010] Furthermore, the diameter of the floating plug is larger than the inner wall diameter of the fluid infusion channel, and the floating plug floats up to the lower end of the fluid infusion channel to plug the fluid infusion channel.

[0011] Beneficial effects of the utility model:

[0012] The utility model provides a partition in the bottle cavity of the mobile phase continuous liquid adding device, which divides the bottle cavity into a main cavity and a liquid replenishment cavity. The bottle cap is provided with a liquid replenishment port leading to the liquid replenishment cavity, and a liquid replenishment channel is provided between the liquid replenishment port and the liquid replenishment cavity. The operator directly replenishes the mobile phase into the liquid replenishment cavity through the liquid replenishment port without opening the bottle cap, so the catheter inserted into the straw port on the bottle cap will not stir up bubbles. The replenished mobile phase flows through the liquid replenishment channel into the liquid replenishment cavity and then replenishes into the main cavity. The replenished mobile phase and the mobile phase in the liquid replenishment cavity mix to generate bubbles, which only float in the liquid replenishment cavity and are blocked by the partition and will not flow into the main cavity. Therefore, the mobile phase in the main cavity is not affected by the bubbles, will not bring noise to the liquid chromatograph, will not cause the obtained chromatogram to appear sharp noise peaks, and will not cause the analytical sensitivity of the liquid chromatograph to decrease. A floating plug is provided in the liquid replenishment cavity. As the mobile phase is replenished, the floating plug floats up to plug the liquid replenishment channel, preventing the mobile phase in the liquid replenishment cavity from backflowing and overflowing, and will not cause the danger of leakage. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a structural schematic diagram of a mobile phase continuous liquid adding device for liquid chromatography of the present invention;

[0014] Figure 2 It is a structural schematic diagram of a glass bottom-mouth bottle used in conjunction with a mobile phase continuous liquid adding device for liquid chromatography provided by the present invention;

[0015] Figure 3 The present invention is a schematic structural diagram of a mobile phase continuous liquid adding device for liquid chromatography in which a bottle cavity is filled with liquid during use.

[0016] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0017] 1. Bottle cavity; 11. Main cavity; 12. Liquid replenishment cavity; 2. Bottle cap; 3. Straw port; 4. Partition; 41. Liquid replenishment channel; 42. Opening at the bottom end of the partition; 5. Glass bottom-mouth bottle; 6. Water outlet pipe with switch valve; 7. Hose; 8. Partial flip cover; 9. Float plug. DETAILED DESCRIPTION

[0018] The present invention is further described in detail below in conjunction with specific implementation methods.

[0019] Liquid chromatography mobile phase continuous liquid addition device such as Figure 1 As shown, it includes a bottle cavity 1 for containing a mobile phase and a bottle cap 2 covering the bottle mouth. The bottle cavity 1 is divided by a partition 4 to form a main cavity 11 and a liquid replenishment cavity 12; a partition bottom opening 42 is provided at the bottom of the partition 4, so that the main cavity 11 and the liquid replenishment cavity 12 are connected at the bottom. The bottle cap 2 is provided with a suction pipe opening 3 leading to the main cavity 11, and the catheter of the liquid chromatograph is inserted through the suction pipe opening 3 to lead into the bottle cavity 1.

[0020] The operator fills the bottle cavity 1 with mobile phase, then covers the bottle cap 2, and the catheter of the liquid chromatograph is inserted through the pipette port 3 to the bottom of the main cavity 11 in the bottle cavity 1. The liquid chromatograph starts working, and the mobile phase is sucked out through the pipette port 3 for use. The mobile phase in the bottle cavity 1 is continuously reduced until the remaining amount of mobile phase is insufficient to complete subsequent detection, and it is necessary to replenish the mobile phase in the device. For this reason, a refilling port leading to the refilling cavity 12 is provided on the bottle cap 2. The bottle cap 2 is provided with a local flip cover 8 at the refilling port, and a refilling channel 41 is provided between the refilling port and the refilling cavity 12, and the refilling channel 41 is a glass cylinder. The operator opens the local flip cover 8 to directly replenish the mobile phase into the refilling cavity 12, and there is no need to open the bottle cap 2, so the catheter inserted through the pipette port 3 on the bottle cap 2 will not be disturbed. The replenished mobile phase flows through the replenishment channel 41 into the replenishment chamber 12 and merges into the main chamber 11. The replenished mobile phase and the mobile phase in the replenishment chamber 12 mix to generate bubbles. These bubbles only float up in the replenishment chamber 12 and are blocked by the partition 4 and will not flow into the main chamber 11. Therefore, the mobile phase in the main chamber 11 is not affected by the added bubbles, avoiding noise to the liquid chromatograph, causing sharp noise peaks to appear in the obtained chromatogram, and not causing a decrease in the analysis sensitivity of the liquid chromatograph.

[0021] A spherical floating plug 9 made of polyvinyl chloride is provided in the liquid replenishment chamber 12, and its diameter is greater than the height of the opening 42 at the bottom of the partition, so that it can float on the liquid surface of the mobile phase. Figure 3 As shown, the spherical float 9 floats to the lower end of the liquid replenishment channel 41 (glass cylinder); since the diameter of the float 9 is larger than the inner wall diameter of the liquid replenishment channel 41, it will float up to plug the liquid replenishment channel 41 to prevent the mobile phase in the liquid replenishment chamber 12 from flowing back and overflowing.

[0022] like Figure 2The glass bottom-mouth bottle 5 shown is used to add the freshly prepared mobile phase into the mobile phase continuous liquid adding device. The lower part of the glass bottom-mouth bottle 5 is provided with an opening, and the outer side wall of the opening is provided with a water outlet pipe 6 with a switch valve, and the freshly prepared mobile phase can flow out from the water outlet of the water outlet pipe 6 with a switch valve under the action of gravity. The outlet of the water outlet pipe 6 with a switch valve is provided with a hose 7, and the outer diameter of the hose 7 matches the inner diameter of the liquid replenishment channel 41. When in use, the hose 7 goes deep into the liquid replenishment channel 41, so that the liquid replenishment channel 41 covers the hose 7, the outer wall of the hose 7 sticks to the inner wall of the liquid replenishment channel 41, and the bottom end of the hose 7 does not exceed the bottom end of the liquid replenishment channel 41. Because the glass bottom-mouth bottle 5, the hose 7 and the liquid replenishment channel 41 form a closed environment, when the floating plug 9 blocks the channel, the mobile phase liquid in the glass bottom-mouth bottle 5 no longer flows down due to gravity, and the liquid addition stops. At this time, the valve can be closed and the bottom-mouth bottle can be moved away.

[0023] The above is only an implementation method of the utility model, and does not limit the scope of patent protection. Those skilled in the art can make non-substantial changes or substitutions based on the utility model, which still fall within the scope of patent protection.

Claims

1. A mobile phase continuous liquid addition device for liquid chromatography, comprising a bottle cavity (1) for containing the mobile phase and a bottle cap (2) for covering the bottle mouth, characterized in that: The bottle cavity (1) is divided by a partition (4) to form a main cavity (11) and a liquid replenishing cavity (12). The bottom of the partition (4) is provided with a partition bottom opening (42). The liquid replenishing cavity (12) and the main cavity (11) are connected at the partition bottom opening (42). The bottle cap (2) is provided with a straw opening (3) leading to the main cavity (11) and a liquid replenishing port leading to the liquid replenishing cavity (12). A liquid replenishing channel (41) is provided between the liquid replenishing port and the liquid replenishing cavity (12). A floating plug (9) is provided in the liquid replenishing cavity (12). As the mobile phase is replenished, the floating plug (9) floats up to plug the liquid replenishing channel (41).

2. The mobile phase continuous liquid adding device according to claim 1, characterized in that: A glass bottom-mouth bottle (5) is provided, the lower part of which is provided with an opening, and an outlet pipe (6) with a switch valve is provided on the outer side wall of the opening, and the mobile phase flows out from the outlet.

3. The mobile phase continuous liquid addition device according to claim 2, characterized in that: A hose (7) is sleeved at the water outlet of the water outlet pipe (6) with a switch valve. The hose (7) extends into the liquid replenishing channel (41). The outer wall of the hose (7) abuts against the inner wall of the liquid replenishing channel (41), and the bottom end of the hose (7) does not exceed the bottom end of the liquid replenishing channel (41).

4. The mobile phase continuous liquid addition device according to claim 3, characterized in that: The outer wall of the hose (7) is attached to the inner wall of the fluid infusion channel (41).

5. The mobile phase continuous liquid adding device according to claim 1, characterized in that: The bottle cap (2) is provided with a partial flip cover (8) at the liquid replenishing port.

6. The mobile phase continuous liquid addition device according to claim 1, characterized in that: The fluid replenishment channel (41) is a glass cylinder.

7. The mobile phase continuous liquid addition device according to claim 6, characterized in that: The floating plug (9) is spherical, and its diameter is greater than the height of the opening (42) at the bottom end of the partition.

8. The mobile phase continuous liquid addition device according to claim 7, characterized in that: The diameter of the floating plug (9) is greater than the inner wall diameter of the fluid infusion channel (41), and the floating plug (9) floats up to the lower end of the fluid infusion channel (41) to plug the fluid infusion channel (41).

Images