Colloidal gold reagent strip for detecting aminoglycoside antibiotics

Abstract

The utility model provides a colloidal gold reagent strip for detecting aminoglycoside antibiotics, which comprises a shell and a colloidal gold reagent strip, and the upper opening of the shell is connected with an upper cover in a sliding manner; a detection chamber is formed in the shell, the interior of the detection chamber is rotatably connected with a driving shaft and a driven shaft, and one end part of the driving shaft penetrates through the inner wall of the detection chamber, extends to the exterior of the shell and forms a screwing part; the surface of the driving shaft is sleeved with one end of a transmission belt, the surface of the driven shaft is sleeved with the other end of the transmission belt, a plurality of colloidal gold reagent strips are pasted on the surface of the transmission belt, and sample adding grooves, observation grooves and type display grooves are formed in the positions, corresponding to the colloidal gold reagent strips, of the top of the upper cover; according to the utility model, the types of required colloidal gold reagent strips can be selected according to different samples, the required reagent strips can be selected for multiple times and the samples can be continuously dropped through the sample adding groove when multiple experiments are carried out, the results of a plurality of reagent strips can appear at the same time, and the experiment efficiency is improved.

Description

Technical Field

[0001] The utility model relates to the field of aminoglycoside antibiotic detection, in particular to a colloidal gold reagent strip used for detecting aminoglycoside antibiotics. Background Art

[0002] Aminoglycoside antibiotics (Aminogly cosides) are glycoside antibiotics formed by connecting amino sugars and aminocyclitols through oxygen bridges. Although most antibiotics that inhibit microbial protein synthesis are bacteriostatic drugs, aminoglycoside antibiotics can have a bactericidal effect and are dormant bactericidal drugs. The detection method for aminoglycoside antibiotics generally uses colloidal gold immunochromatography, with colloidal gold as a tracer marker. After extraction, the sample is combined with a specific antibody labeled with colloidal gold, thereby inhibiting the antibody from binding to the antigen on the test line (T line) of the test strip, resulting in a change in the color depth of the test line (T line).

[0003] In the related art, a utility model patent with patent announcement number CN204719028U was searched and proposed a synchronous detection card for detecting five aminoglycoside antibiotics, including a detection card and a dropper. The detection card is composed of a test strip and a card shell. The test strip is composed of a PVC plate, a sample pad, a colloidal gold pad, a nitrocellulose membrane, a blotting paper, a protective film and a label film. Neomycin, streptomycin, gentamicin, spectinomycin and kanamycin monoclonal antibodies are respectively labeled with colloidal gold, freeze-dried onto a glass cellulose membrane to make a colloidal gold pad, and the nitrocellulose membrane is coated with the above five aminoglycoside antibiotic hapten-ovalbumin conjugates as the detection line, and coated with sheep anti-mouse as the quality control line. The five test strips are placed in a card shell, and there is a sample loading slot on the card shell for loading samples, and neomycin, streptomycin, gentamicin, spectinomycin and kanamycin are detected simultaneously.

[0004] According to the above-mentioned prior art, the inventors combined with relevant technologies to find that in actual testing, the types and quantities of test strips required are different depending on the samples. Since the utility model transports the samples to all test strips through a non-woven fabric, direct use will cause a huge waste of test strips, and in the face of different samples, it is necessary to frequently open the test kit to readjust the type and quantity of test strips, which is cumbersome and inconvenient to use. Utility Model Content

[0005] In order to solve the problem in the prior art that a large number of reagent strips are wasted and the reagent kit needs to be frequently opened to readjust the type and quantity of reagent strips for different samples, the utility model provides a colloidal gold reagent strip for detecting aminoglycoside antibiotics;

[0006] The utility model provides a colloidal gold reagent strip for detecting aminoglycoside antibiotics using the following technical solution:

[0007] A colloidal gold reagent strip for detecting aminoglycoside antibiotics, comprising a shell and a colloidal gold reagent strip, wherein an upper cover is slidably connected to the upper opening of the shell; a detection chamber is formed inside the shell, a driving shaft and a driven shaft are rotatably connected inside the detection chamber, one end of the driving shaft passes through the inner wall of the detection chamber and extends to the outside of the shell and forms a screwing portion; one end of a transmission belt is sleeved on the surface of the driving shaft, the other end of the transmission belt is sleeved on the surface of the driven shaft, a plurality of colloidal gold reagent strips are pasted on the surface of the transmission belt, and a sample addition slot, an observation slot and a type display slot are respectively opened at the top of the upper cover at positions corresponding to the colloidal gold reagent strip;

[0008] Furthermore, the circumferential surfaces of the driving shaft and the driven shaft are respectively provided with extensions with diameters larger than the driving shaft and the driven shaft, and the driving shaft and the driven shaft are connected to the transmission belt through the extensions;

[0009] Furthermore, two opposite outer walls of the shell are provided with slide grooves near the upper opening, and two sides of the bottom of the upper cover are provided with sliding parts, and the upper cover is slidably connected in the slide grooves through the sliding parts;

[0010] Furthermore, the housing further comprises a first chamber and a second chamber, wherein a plurality of replaceable colloidal gold reagent strips are placed in the first chamber, and a tweezers groove for placing tweezers is provided at the bottom of the second chamber;

[0011] Furthermore, a draw groove is provided on the side wall of the shell, the draw groove is located at the lower part of the first chamber and the second chamber, a draw plate is slidably connected in the draw groove, and a test tube groove for placing a diluent test tube and a cotton swab groove for placing a cotton swab are respectively provided on the surface of the draw plate;

[0012] Furthermore, a plurality of ridges for increasing friction are provided on the side wall of the screwing portion;

[0013] Furthermore, the surface of the transmission belt and the bottom surface of the colloidal gold reagent strip are both provided with Velcro.

[0014] In summary, the beneficial effects of the utility model are:

[0015] The utility model provides a sample adding slot, an observation slot and a type display slot on the top of the upper cover, so that detection and observation results can be performed without opening the upper cover, thereby reducing the risk of the inside of the shell being polluted by the external environment; by providing a driving shaft, a driven shaft and a transmission belt inside the shell, the type of colloidal gold reagent strip required can be selected according to different samples without opening the shell, and when performing multiple experiments, the required reagent strips can be continuously selected through the screwing part and the samples can be continuously dripped into the sample adding slot, and the results of multiple reagent strips can appear almost at the same time, thereby improving convenience and experimental speed; by providing a first chamber, a second chamber and a pull-out plate, the necessary tools required for the experiment can be provided, thereby improving practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the main view of the utility model in an open state;

[0017] Figure 2 It is a perspective schematic diagram of the utility model;

[0018] Figure 3 It is a cross-sectional schematic diagram of the utility model;

[0019] Figure 4 It is a top view schematic diagram of the utility model.

[0020] As shown in the figure: 1-shell, 11-slide groove, 12-sliding part, 2-colloidal gold reagent strip, 3-upper cover, 4-detection chamber, 41-driving shaft, 42-driven shaft, 43-screwing part, 44-transmission belt, 45-extension part, 51-sample adding slot, 52-observation slot, 53-type display slot, 6-first chamber, 7-second chamber, 71-tweezers slot, 8-drawing slot, 81-pull-out plate, 82-test tube slot, 83-cotton swab slot. DETAILED DESCRIPTION

[0021] The following is combined with Figure 1 -Attached Figure 4 The utility model is further described in detail:

[0022] The utility model embodiment discloses a colloidal gold reagent strip for detecting aminoglycoside antibiotics, such as Figure 1 , Figure 2 , Figure 4As shown, a colloidal gold reagent strip for detecting aminoglycoside antibiotics of the utility model comprises a housing 1 and a colloidal gold reagent strip 2, wherein an upper cover 3 is slidably connected to the upper opening of the housing 1; a detection chamber 4 is formed inside the housing 1, and a driving shaft 41 and a driven shaft 42 are rotatably connected inside the detection chamber 4, wherein one end of the driving shaft 41 passes through the inner wall of the detection chamber 4 and extends to the outside of the housing 1 and is formed with a screwing portion 43; one end of a transmission belt 44 is sleeved on the surface of the driving shaft 41, and the other end of the transmission belt 44 is sleeved on the driven shaft 42 Surface, a number of colloidal gold reagent strips 2 are pasted on the surface of the transmission belt 44, and a sample addition slot 51, an observation slot 52 and a type display slot 53 are respectively opened at the top of the upper cover 3 corresponding to the colloidal gold reagent strips 2; in this embodiment, the shell 1 is a hollow structure, and the inner wall of the detection chamber 4 is rotatably connected with a driving shaft 41 and a driven shaft 42 through a bearing, one end of the driving shaft 41 passes through the inner wall of the detection chamber 4 and extends to the outside of the shell 1 and is formed with a screwing portion 43, and preferably, the side wall of the screwing portion 43 is provided with a ridge for increasing friction. By arranging a sample adding slot 51, an observation slot 52 and a type display slot 53 on the top of the upper cover 3, detection can be directly performed and the results can be observed, wherein the sample adding slot 51 corresponds to the sample pad of the colloidal gold reagent strip 2, the observation slot 52 corresponds to the detection line area of ​​the colloidal gold reagent strip 2, and the type display slot 53 corresponds to the absorbent pad at the end of the colloidal gold reagent strip 2. It is worth noting that the surface of the absorbent pad is technically marked with the detection type of the colloidal gold reagent strip 2. The advantage of this is that the colloidal gold reagent strip 2 that you want to use to detect the sample can be selected by rotating the screw part 43. By setting a driving shaft 41, a driven shaft 42 and a transmission belt 44, the colloidal gold reagent strip 2 can be pasted on the surface of the transmission belt 44, and the driving shaft 41 can be driven to rotate by turning the screwing part 43. At this time, the colloidal gold reagent strip 2 will make a circular motion on the surface of the transmission belt 44 along with the transmission belt 44, and the type of the colloidal gold reagent strip 2 can be determined by the observation groove 52. It is worth noting that multiple colloidal gold reagent strips 2 are not in contact with each other on the surface of the transmission belt 44 but have a certain interval, and no contamination will be generated when the sample is dripped. Therefore, the sample addition groove 51, the observation groove 52 and the type display groove 53 on the surface of the upper cover 3 can be set to N groups, N≥1. Preferably, the surface of the transmission belt 44 and the bottom surface of the colloidal gold reagent strip 2 are both provided with Velcro.

[0023] like Figure 1 , Figure 3As shown, the circumferential surfaces of the driving shaft 41 and the driven shaft 42 are respectively provided with extensions 45 whose diameters are larger than those of the driving shaft 41 and the driven shaft 42, and the driving shaft 41 and the driven shaft 42 are transmission-connected with the transmission belt 44 through the extensions 45; in this embodiment, the extension 45 is a cylinder whose diameter is larger than that of the driving shaft 41 and the driven shaft 42 and is coaxial with the driving shaft 41 and the driven shaft 42, and its main function is to form a transmission connection with the transmission belt 44 through its rough surface and to shorten the distance between the colloidal gold reagent strip 2 and the upper cover 3 at the same time.

[0024] like Figure 1 , Figure 2 , Figure 3 As shown, two opposite outer walls of the housing 1 are provided with slide grooves 11 near the upper opening, and two sides of the bottom of the upper cover 3 are provided with sliding parts 12, and the upper cover 3 is slidably connected in the slide grooves 11 through the sliding parts 12; in this embodiment, the specific shape of the sliding part 12 is as follows Figure 3 As shown, two sliding parts 12 are provided at the bottom of the upper cover 3, and the two sliding parts 12 are slidably connected to the inside of the slide groove 11 from relative horizontal positions. The advantage of this is that the structure of the upper cover 3 ensures that the sliding part 12 is always in the slide groove 11, and the upper cover 3 will not be separated from the shell 1 when the upper cover 3 is not deformed.

[0025] like Figure 1 , Figure 2 , Figure 3 As shown, the housing 1 further includes a first chamber 6 and a second chamber 7. A plurality of replaceable colloidal gold reagent strips 2 are placed in the first chamber 6. A tweezer slot 71 for placing tweezers is provided at the bottom of the second chamber 7. In this embodiment, the first chamber 6 is used to store replaceable colloidal gold reagent strips 2. The types of colloidal gold reagent strips 2 include but are not limited to streptomycin, gentamicin, tobramycin, amikacin, amikacin, and netilmicin. The second chamber 7 stores tweezers through the tweezer slot 71 to avoid direct contact with hands.

[0026] like Figure 1 , Figure 2 As shown, a drawer groove 8 is provided on the side wall of the shell 1, and the drawer groove 8 is located at the lower part of the first chamber 6 and the second chamber 7. A drawer plate 81 is slidably connected in the drawer groove 8, and a test tube groove 82 for placing a diluent test tube and a cotton swab groove 83 for placing a cotton swab are respectively provided on the surface of the drawer plate 81; in this embodiment, by adding the drawer plate 81, the necessary tools required for the experiment, such as: diluent, cotton swabs, etc., can be stored, thereby improving practicality.

[0027] The implementation principle of the embodiment of the utility model is:

[0028] The screwing part 43 is turned to select the colloidal gold reagent strip 2 of the required type, and the sample is mixed with the diluent and then dripped into the sample adding slot 51. When multiple experiments are performed, the required colloidal gold reagent strip 2 is continuously selected by the screwing part 43 and the sample is continuously dripped into the sample adding slot 51, and the sample is left to stand for a period of time and the result is observed through the observation slot 52. The results of multiple colloidal gold reagent strips 2 can appear at the same time; when the colloidal gold reagent strip 2 needs to be replaced, the upper cover 3 is slid to expose the transmission belt 44 inside the shell 1, the used colloidal gold reagent strip 2 is removed, and the colloidal gold reagent strip 2 in the first chamber 6 is clamped by the tweezers in the tweezers slot 71, and the colloidal gold reagent strip 2 is attached to the surface of the transmission belt 44 by Velcro.

[0029] The above shows and describes the basic principle and main features of the utility model and the advantages of the utility model. The various components mentioned in the utility model are common technologies in the existing field. The technicians in this industry should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only to illustrate the principles of the utility model. Without departing from the spirit and scope of the utility model, the utility model will have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.

Claims

1. A colloidal gold reagent strip for detecting aminoglycoside antibiotics, comprising a housing (1) and a colloidal gold reagent strip (2), characterized in that: An upper cover (3) is slidably connected to the upper opening of the shell (1); a detection chamber (4) is formed inside the shell (1); a driving shaft (41) and a driven shaft (42) are rotatably connected inside the detection chamber (4); one end of the driving shaft (41) passes through the inner wall of the detection chamber (4) and extends to the outside of the shell (1) and is formed with a screwing portion (43); one end of a transmission belt (44) is sleeved on the surface of the driving shaft (41); the other end of the transmission belt (44) is sleeved on the surface of the driven shaft (42); a plurality of colloidal gold reagent strips (2) are adhered to the surface of the transmission belt (44); and a sample loading slot (51), an observation slot (52) and a type display slot (53) are respectively provided at the top of the upper cover (3) and at positions corresponding to the colloidal gold reagent strips (2).

2. A colloidal gold reagent strip for detecting aminoglycoside antibiotics according to claim 1, characterized in that: The circumferential surfaces of the driving shaft (41) and the driven shaft (42) are respectively provided with extensions (45) having a diameter greater than that of the driving shaft (41) and the driven shaft (42); the driving shaft (41) and the driven shaft (42) are connected to a transmission belt (44) via the extensions (45).

3. A colloidal gold reagent strip for detecting aminoglycoside antibiotics according to claim 2, characterized in that: Two opposite outer walls of the shell (1) are respectively provided with sliding grooves (11) near the upper opening, and two sides of the bottom of the upper cover (3) are respectively provided with sliding parts (12), and the upper cover (3) is slidably connected in the sliding grooves (11) via the sliding parts (12).

4. A colloidal gold reagent strip for detecting aminoglycoside antibiotics according to claim 1 or 3, characterized in that: The shell (1) is further provided with a first chamber (6) and a second chamber (7), wherein a plurality of replaceable colloidal gold reagent strips (2) are placed inside the first chamber (6), and a tweezer groove (71) for placing tweezers is provided at the bottom of the second chamber (7).

5. A colloidal gold reagent strip for detecting aminoglycoside antibiotics according to claim 4, characterized in that: A draw-out groove (8) is provided on the side wall of the shell (1), and the draw-out groove (8) is located at the lower part of the first chamber (6) and the second chamber (7). A draw-out plate (81) is slidably connected in the draw-out groove (8), and a test tube groove (82) for placing a diluent test tube and a cotton swab groove (83) for placing a cotton swab are respectively provided on the surface of the draw-out plate (81).

6. A colloidal gold reagent strip for detecting aminoglycoside antibiotics according to claim 2, characterized in that: A plurality of ridges for increasing friction are arranged on the side wall of the screwing portion (43).

7. A colloidal gold reagent strip for detecting aminoglycoside antibiotics according to claim 1 or 5, characterized in that: The surface of the transmission belt (44) and the bottom surface of the colloidal gold reagent strip (2) are both provided with Velcro.

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