Anti-splash flow channel structure and centrifugal medical microfluidic chip

Abstract

The utility model discloses an anti-splashing flow channel structure and a centrifugal medical microfluidic chip, wherein the anti-splashing flow channel structure comprises: a zigzag microchannel, comprising a liquid inlet section, a reversing section and a liquid outlet section which are connected end to end in sequence, wherein the liquid inlet section is used for allowing a fluid sample to be tested to flow in; the liquid outlet section is used for connecting to a reaction chamber containing a reagent that can splash; the reversing section is used for guiding the fluid sample to be tested in the liquid inlet section to flow into the liquid outlet section; and an inner concave space, wherein the inner concave space is at least partially formed on a side wall of the reversing section which is arranged corresponding to the liquid outlet section; when the reagent that can splash out flows out of the reaction chamber and flows back through the liquid outlet section, the inner concave space intercepts the reagent that can splash out.

Description

Technical Field

[0001] The utility model relates to the technical field of medical microfluidic chips, in particular to an anti-splashing flow channel structure and a centrifugal medical microfluidic chip. Background Art

[0002] Microfluidic chip technology is a technology that controls the flow of liquids in micron-sized channels. It is a mainstream trend in the transformation of analytical technology in the 21st century and has gradually developed into a cutting-edge technology that integrates many disciplines such as fluid mechanics, materials, mechanics, electronics, physics, chemistry and biology. Microfluidic chip technology has the advantages of low reagent and sample consumption, fast, low-cost, high throughput and easy use. It is widely used in many fields such as disease diagnosis, drug screening, environmental monitoring, food safety, material synthesis and on-site detection.

[0003] Due to the high integration of microfluidic chips, multiple reaction chambers are often designed for one chip, and different reagents are pre-set in multiple reaction chambers to achieve the detection of multiple indicators of the same sample. During the detection, the sample to be detected enters each reaction chamber through the diversion channel and the injection channel in turn.

[0004] In the prior art, the dispensing channel usually adopts a linear channel. If the reagent pre-installed in the reaction chamber is liquid, there is a risk of the liquid reagent splashing from the reaction chamber to the diversion channel when it is bumped during transportation or dropped during use, thereby causing cross contamination between the reaction chambers and affecting the accuracy of the detection.

[0005] The information disclosed in this background technology section is only intended to increase the understanding of the overall background of the utility model, and should not be regarded as acknowledging or suggesting in any form that the information constitutes the prior art already known to ordinary technicians in this field. Utility Model Content

[0006] Purpose of the utility model: The technical problem to be solved by the utility model is to provide a splash-proof flow channel structure and a centrifugal medical microfluidic chip to reduce the risk of direct splashing without affecting the entry of the fluid sample to be tested into the reaction chamber in response to the deficiencies of the prior art.

[0007] In order to solve the above technical problems, the first aspect of the utility model discloses a splash-proof flow channel structure, which includes:

[0008] A zigzag microfluidic channel comprises a liquid inlet section, a reversing section and a liquid outlet section which are connected end to end in sequence, wherein the liquid inlet section is used for the inflow of a fluid sample to be tested; the liquid outlet section is used for connecting to a reaction chamber containing a splashable reagent; and the reversing section is used for guiding the fluid sample to be tested in the liquid inlet section to flow into the liquid outlet section;

[0009] and a concave space, wherein the concave space is at least partially formed on a side wall of the reversing section corresponding to the liquid outlet section;

[0010] When the splashable reagent flows out of the reaction chamber and flows back through the liquid outlet section, the inner concave space retains the splashable reagent.

[0011] In some examples, the concave space includes a first straight side wall, a second straight side wall, and an arc transition surface connecting the first straight side wall and the second straight side wall, the first straight side wall is directly connected to the side wall of the corresponding side of the liquid outlet section and the two are arranged in the same plane. The first straight side wall and the second straight side wall form a first angle away from the reversing section, and the value range of the first angle is 45-120°.

[0012] Preferably, the first angle has a value range of 45-90°.

[0013] In some examples, a center line of the liquid inlet section and a center line of the reversing section form a second angle, and a value range of the second angle is 45-120°.

[0014] Preferably, the second angle is in the range of 90-120°.

[0015] In some examples, the center line of the liquid inlet section is arranged parallel to the center line of the liquid outlet section.

[0016] In some examples, the liquid inlet section includes an open section for the inflow of the fluid sample to be tested and a straight section for connecting to the reversing section. The flow channel width of the open section gradually decreases from the inflow direction of the fluid sample to be tested. In some examples, the cross-sectional shape of the open section is a trapezoid.

[0017] In some examples, the flow channel width of the reversing section is greater than the flow channel width of the liquid inlet section. The connection between the side wall surface of the reversing section and the side wall surface of the liquid inlet section and the connection between the side wall surface of the reversing section and the side wall surface of the liquid outlet section are both arc transitions.

[0018] The second aspect of the present invention discloses a centrifugal medical microfluidic chip, which includes a flow guide channel, a reaction chamber, and an anti-splashing flow channel structure, wherein the reaction chamber is connected to the flow guide channel through the anti-splashing flow channel structure. The flow guide channel is an arc-shaped flow channel whose arc center is located on the rotation center axis of the microfluidic chip. The reaction chamber is located on the side of the flow guide channel away from the rotation center axis of the microfluidic chip. The anti-splashing flow channel structure is arranged between the reaction chamber and the flow guide channel.

[0019] Beneficial effects:

[0020] The splash-proof flow channel structure disclosed in the utility model is not a traditional straight-line type, but is designed as a zigzag micro-channel, and a concave space is provided at the same time. Due to the presence of the concave space, when the pre-set splashable reagent is splashed by external force, it will be trapped by the concave space, which effectively reduces the risk of direct splashing, and does not affect the fluid sample to be tested from entering the reaction chamber. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific implementation methods, and the above and / or other advantages of the present invention will become more clear.

[0022] Figure 1 A schematic diagram of the three-dimensional structure of a centrifugal medical microfluidic chip disclosed in one embodiment of the utility model;

[0023] Figure 2 for Figure 1 A top view of a centrifugal medical microfluidic chip is shown;

[0024] Figure 3 for Figure 2 A partial enlarged view of a centrifugal medical microfluidic chip is shown, showing a splash-proof flow channel structure;

[0025] FIG. 4( a ) and FIG. 4( b ) are comparison diagrams of drop test results of a centrifugal medical microfluidic chip having an anti-splashing flow channel structure and a centrifugal medical microfluidic chip having a straight flow channel type diversion flow channel.

[0026] The description of the accompanying drawings is:

[0027] 100, anti-splashing flow channel structure; 110, liquid inlet section; 120, reversing section; 130, liquid outlet section; 140, concave space; 141, first straight side wall surface; 142, second straight side wall surface; 143, arc transition surface; 200, reaction chamber; 300, flow guide channel; α, first angle; β, second angle. DETAILED DESCRIPTION

[0028] Example 1

[0029] Referring to FIG. 4( b ), in the prior art, the dispensing channel usually adopts a straight channel. If the reagent pre-installed in the reaction chamber is liquid, there is a risk that the liquid reagent will splash from the reaction chamber to the diversion channel when it is bumped during transportation or dropped during use, thereby causing cross contamination between the reaction chambers and affecting the accuracy of the detection.

[0030] Therefore, see Figures 1 to 3 This embodiment discloses a splash-proof channel structure, wherein the splash-proof channel structure 100 includes a zigzag microchannel and an inner concave space 140 .

[0031] See also Figure 3 The zigzag microchannel includes a liquid inlet section 110, a reversing section 120 and a liquid outlet section 130 connected end to end in sequence. The liquid inlet section 110 is used for the inflow of the fluid sample to be tested. The liquid outlet section 130 is used to connect the reaction chamber 200 containing the splashable reagent. The reversing section 120 is used to guide the fluid sample to be tested in the liquid inlet section 110 to flow into the liquid outlet section 130. The liquid inlet section 110, the reversing section 120 and the liquid outlet section 130 are all straight channels as a whole.

[0032] The concave space 140 is at least partially formed on a side wall of the reversing section 120 that is arranged corresponding to the liquid outlet section 130 .

[0033] When the splashable reagent flows out of the reaction chamber 200 and flows back through the liquid outlet section 130, the splashable reagent flows directly along the liquid outlet section 130 until it reaches the inside of the concave space 140. The concave space 140 retains the splashable reagent.

[0034] For some examples, see Figure 3 The concave space 140 includes a first straight side wall surface 141, a second straight side wall surface 142, and an arc transition surface 143 connecting the first straight side wall surface 141 and the second straight side wall surface 142. The first straight side wall surface 141 is directly connected to the side wall surface on the corresponding side of the liquid outlet section 130, and the two are arranged in the same plane. The first straight side wall surface 141 and the second straight side wall surface 142 form a first angle α away from the reversing section 120, and the value range of the first angle α is 45-120°.

[0035] Preferably, the value range of the first angle α is 45-90°.

[0036] For some examples, see Figure 3 The center line of the liquid inlet section 110 and the center line of the reversing section 120 form a second angle β, and the value range of the second angle β is 45-120°.

[0037] Preferably, the second angle β is in the range of 90-120°.

[0038] For some examples, see Figure 3 The center line of the liquid inlet section 110 is arranged parallel to the center line of the liquid outlet section 130 .

[0039] For some examples, see Figure 3 The liquid inlet section 110 includes an open section 111 for the inflow of the fluid sample to be tested and a straight section 112 for connecting with the reversing section 120. The flow channel width of the open section 111 gradually decreases from the inflow direction of the fluid sample to be tested.

[0040] This embodiment allows the fluid sample to enter the reaction chamber better by providing the open segment 111. For the fluid sample to enter the reaction chamber containing particles, the open segment 111 allows the sample to enter the reaction chamber evenly.

[0041] For some examples, see Figure 3 , viewed from a top view, the cross-sectional shape of the open segment 111 is generally trapezoidal.

[0042] For some examples, see Figure 3 The flow channel width of the reversing section 120 is greater than the flow channel width of the liquid inlet section 110 to facilitate the inflow of the fluid sample to be tested and to provide a certain retention space for the splashed reagent.

[0043] See also Figure 3 The connection between the side wall surface of the reversing section 120 and the side wall surface of the liquid inlet section 110 and the connection between the side wall surface of the reversing section 120 and the side wall surface of the liquid outlet section 130 are both arc transitions.

[0044] See also Figure 1 to Figure 2 The present embodiment also discloses a centrifugal medical microfluidic chip, which includes a flow guide channel 300, a reaction chamber 200, and the above-mentioned anti-splashing flow channel structure 100, and the reaction chamber 200 is connected to the flow guide channel 300 through the anti-splashing flow channel structure 100. The flow guide channel 300 is an arc-shaped flow channel whose arc center is located on the rotation center axis of the microfluidic chip. The reaction chamber 200 is located on the side of the flow guide channel 300 away from the rotation center axis of the microfluidic chip. The anti-splashing flow channel structure 100 is arranged between the reaction chamber 200 and the flow guide channel 300.

[0045] In a specific embodiment, as shown in Fig. 4(a), the first angle α is 45°, and the second angle β is 120°. The reaction chamber 200 has a volume of 30ul, in which 6ul of blue liquid is preset as methylene blue solution.

[0046] The splash-proof flow channel structure 100 disclosed in the utility model is compared and verified with the straight flow channel structure:

[0047] This verification is a free-fall drop test of the chip, in which the reaction chamber volume is 30ul, and the preset 6ul blue liquid is a methylene blue solution. The results of the drop test are shown in Figures 4(a) and 4(b). Under the free-fall test under the same conditions, see the red circle area in Figure 4(a), the liquid does not splash out from the anti-splash flow channel structure 100 to the diversion flow channel. On the contrary, see the red circle area in Figure 4(b), part of the liquid splashes out through the linear flow channel to the diversion flow channel. It can be seen from the test results that the anti-splash flow channel structure 100 of the utility model can effectively prevent the preset liquid in the reaction chamber from splashing out to the diversion flow channel.

[0048] The utility model provides a splash-proof flow channel structure and a centrifugal medical microfluidic chip. There are many methods and ways to implement the technical solution. The above is only the preferred implementation of the utility model. It should be pointed out that for ordinary technicians in this technical field, without departing from the principle of the utility model, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the utility model. All components not specified in this embodiment can be implemented by existing technologies.

Claims

1. A splash-proof flow channel structure, characterized in that: include: A zigzag microfluidic channel comprises a liquid inlet section (110), a reversing section (120) and a liquid outlet section (130) connected end to end in sequence, wherein the liquid inlet section (110) is used for allowing a fluid sample to flow in; the liquid outlet section (130) is used for connecting to a reaction chamber (200) containing a splashable reagent; and the reversing section (120) is used for guiding the fluid sample to flow in the liquid inlet section (110) into the liquid outlet section (130); and a concave space (140), wherein the concave space (140) is at least partially formed on a side wall of the reversing section (120) that is arranged corresponding to the liquid outlet section (130); When the splashable reagent flows out of the reaction chamber (200) and flows back through the liquid outlet section (130), the inner concave space (140) retains the splashable reagent.

2. The splash-proof flow channel structure according to claim 1, characterized in that: The concave space (140) comprises a first straight side wall surface (141), a second straight side wall surface (142), and an arc transition surface (143) connecting the first straight side wall surface (141) and the second straight side wall surface (142); the first straight side wall surface (141) is directly connected to the side wall surface on the corresponding side of the liquid outlet section (130), and the two are arranged in the same plane; the first straight side wall surface (141) and the second straight side wall surface (142) form a first angle (α) away from the reversing section (120), and the value range of the first angle (α) is 45-120°.

3. The splash-proof flow channel structure according to claim 2, characterized in that: The first angle (α) has a value range of 45-90°.

4. The anti-splashing flow channel structure according to claim 3, characterized in that: The center line of the liquid inlet section (110) and the center line of the reversing section (120) form a second angle (β), and the value range of the second angle (β) is 45-120°.

5. The splash-proof flow channel structure according to claim 4, characterized in that: The second angle (β) has a value range of 90-120°.

6. The splash-proof flow channel structure according to claim 1, characterized in that: The center line of the liquid inlet section (110) and the center line of the liquid outlet section (130) are arranged in parallel.

7. The splash-proof flow channel structure according to claim 1, characterized in that: The liquid inlet section (110) comprises an open section (111) for allowing the fluid sample to flow in and a straight section (112) for connecting to the reversing section (120); the flow channel width of the open section (111) gradually decreases from the inflow direction of the fluid sample to be tested.

8. The splash-proof flow channel structure according to claim 7, characterized in that: The cross-sectional shape of the open segment (111) is a trapezoid.

9. The splash-proof flow channel structure according to claim 1, characterized in that: The flow channel width of the reversing section (120) is greater than the flow channel width of the liquid inlet section (110); the connection between the side wall surface of the reversing section (120) and the side wall surface of the liquid inlet section (110) and the connection between the side wall surface of the reversing section (120) and the side wall surface of the liquid outlet section (130) are both arc transitions.

10. A centrifugal medical microfluidic chip, characterized in that: The invention comprises a flow guide channel (300), a reaction chamber (200) and an anti-splashing flow channel structure according to claim 1, wherein the reaction chamber (200) is connected to the flow guide channel (300) through the anti-splashing flow channel structure; the flow guide channel (300) is an arc-shaped flow channel whose arc center is located on the rotation center axis of the microfluidic chip; the reaction chamber (200) is located on a side of the flow guide channel (300) away from the rotation center axis of the microfluidic chip; and the anti-splashing flow channel structure is arranged between the reaction chamber (200) and the flow guide channel (300).

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