Culture container for plant tissue culture

Abstract

The utility model discloses a culture container for plant tissue culture, which comprises an upper barrel body and a lower barrel body, an opening of the upper barrel body and an opening of the lower barrel body are embedded and sleeved, and the upper barrel body and the lower barrel body are connected to form a sealed culture cavity; a first sealing ring and a second sealing ring are arranged between the outer wall of the lower barrel body and the inner wall of the upper barrel body, an annular groove for mounting the first sealing ring and the second sealing ring is formed in the lower barrel body, and the first sealing ring and the second sealing ring are fixed in the annular groove; a vent hole is formed in the top of the upper can body, and a detachable breathable film is arranged in the vent hole. The upper barrel body and the lower barrel body are barrel-shaped, a bottleneck part of a traditional tissue culture bottle is not arranged, and the space in the upper barrel body is large, so that explant inoculation is facilitated, and growing tissue culture seedlings cannot be mechanically damaged; the upper barrel body can be telescopically adjusted in height on the lower barrel body, and the tissue culture bottle is more suitable for tissue culture seedlings with different growth heights.

Description

Technical Field

[0001] The utility model relates to the field of plant tissue culture, in particular to a culture container for plant tissue culture. Background Art

[0002] Plant tissue culture refers to the cultivation of plant organs, tissues, cells, embryos, and protoplasts on artificially prepared culture media under sterile conditions, and the induction of callus tissue, latent buds, or complete plants under appropriate culture conditions. These are collectively referred to as plant tissue culture. Plant tissue culture technology has been widely used in genetic seedling breeding, seed resource protection, tissue culture detoxification and rapid propagation.

[0003] Traditional tissue culture containers for plant tissue culture are tissue culture bottles with narrowed mouths or high bodies. These two tissue culture containers have the following problems when performing tissue culture operations:

[0004] 1. The mouth of one tissue culture bottle is relatively small, and the body of the other tissue culture bottle is relatively deep, which is not convenient for explant inoculation and has a relatively low inoculation efficiency;

[0005] 2. When the tissue culture seedlings grow to a certain height, the shrinking bottles will cause crowding, and the shrinking bottles will encounter greater resistance when the tissue culture seedlings are taken out, which is easy to cause mechanical damage and reduce the utilization rate of the tissue culture seedlings;

[0006] 3. The tissue culture seedlings in the tissue culture bottle will grow, and different tissue culture seedlings require different growth heights. The height of the traditional tissue culture bottle cannot be adjusted to adapt to tissue culture seedlings of different growth heights.

[0007] Therefore, it is necessary to propose a culture container for plant tissue culture. Summary of the invention

[0008] In view of the above-mentioned deficiencies in the prior art, the utility model provides a culture container for plant tissue culture.

[0009] In order to achieve the above-mentioned purpose of the invention, the technical solution adopted by the utility model is: it includes an upper barrel body and a lower barrel body, the opening of the upper barrel body and the opening of the lower barrel body are set in a sleeve, and the upper barrel body and the lower barrel body are connected to form a sealed culture chamber; a first sealing ring and a second sealing ring are arranged between the outer wall of the lower barrel body and the inner wall of the upper barrel body, and an annular groove for installing the first sealing ring and the second sealing ring is arranged on the lower barrel body, and the first sealing ring and the second sealing ring are fixed in the annular groove; an air hole is arranged on the top of the upper barrel body, and a detachable air permeable membrane is arranged in the air hole.

[0010] Furthermore, a breathable cylinder is fixed on the breathable hole, a limiting ring supporting the bottom of the breathable membrane is arranged inside the breathable cylinder, a film pressing cylinder for squeezing and supporting the top of the breathable membrane is arranged inside the breathable cylinder, and the inner wall of the breathable cylinder is threadedly connected with the outer wall of the film pressing cylinder.

[0011] Furthermore, the film pressing cylinder includes a knob ring portion and a threaded cylinder portion cooperating with the air-permeable cylinder, the knob ring portion and the threaded cylinder portion are integrally formed, and an anti-slip pattern is arranged on the outside of the knob ring portion.

[0012] Furthermore, the first sealing ring and the second sealing ring have the same structure, and the first sealing ring and the second sealing ring are bonded and fixed in the annular groove.

[0013] Furthermore, the lower barrel body includes a cylindrical upper sealing portion and a lower conical portion, the upper sealing portion and the lower conical portion are integrally formed, and the first sealing ring and the second sealing ring are arranged on the outer side of the upper sealing portion.

[0014] Furthermore, the air cylinder and the upper barrel body are integrally formed.

[0015] Furthermore, a base is provided at the bottom of the lower barrel body, and a radius of the base is greater than a radius of the bottom of the lower barrel body.

[0016] Furthermore, an anti-slip scale marking line is arranged on the outside of the lower barrel, and the anti-slip scale marking line is arranged below the second sealing ring.

[0017] Furthermore, the upper barrel body and the lower barrel body are clearance-matched.

[0018] Further, the radius of the annular groove is equal to the cross-sectional radius of the first sealing ring, and the depth of the annular groove is equal to the cross-sectional radius of the first sealing ring.

[0019] The beneficial effects of the utility model are:

[0020] The upper barrel body of the utility model can be telescopically adjusted on the lower barrel body. By adjusting the telescopic height of the upper barrel body and cooperating with the air holes on the top of the upper barrel body, the air required by the tissue culture plants can be better provided, so that the tissue culture container can be suitable for the production needs of tissue culture plants of different heights. At the same time, the upper barrel body can be removed from the lower barrel body, and the tissue culture plants can be operated through the top of the lower barrel body. Compared with traditional tissue culture bottles, tissue culture operations are more convenient.

[0021] The utility model has an upper barrel body provided with air holes, and a replaceable air permeable membrane is provided on the air holes, through which the oxygen required by tissue culture plants can be provided to the culture cavity, while preventing foreign bacteria from entering the culture cavity; the sealed culture cavity composed of the upper barrel body and the lower barrel body cooperates with the air permeable membrane to provide the required air to the cultured plants in the culture cavity.

[0022] The upper barrel body of the utility model can be telescopically moved outside the lower barrel body through the first sealing ring and the second sealing ring. During the movement, the lower barrel body plays a role similar to a piston. When the upper barrel body reciprocates and telescopes on the lower barrel body, the air in the culture chamber is sucked in and out in cooperation with the air holes and the air permeable membrane on the upper barrel body, so that the culture chamber can be ventilated by utilizing the relative telescopic effect between the upper barrel body and the lower barrel body.

[0023] The upper barrel body and the lower barrel body of the utility model are both in the shape of a barrel, without the bottleneck of a traditional tissue culture bottle, and the space in the upper barrel body is large, which is convenient for inoculation of explants and will not cause mechanical damage to the growing tissue culture seedlings; the upper barrel body can be telescopically adjusted in height on the lower barrel body, and the tissue culture bottle is more suitable for tissue culture seedlings of different growth heights. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0025] Figure 2 It is a structural schematic diagram of the lower barrel;

[0026] Figure 3 It is a top view of the upper barrel;

[0027] Figure 4 for Figure 3 AA section view in;

[0028] The symbols of the components are as follows:

[0029] 1. Upper barrel; 11. Air hole; 12. Air cylinder; 13. Limiting ring; 14. Air membrane; 15. Film pressing cylinder; 16. Guide ring;

[0030] 2. lower barrel; 21. upper sealing portion; 22. lower conical portion;

[0031] 3. First sealing ring; 4. Second sealing ring; 5. Base; 6. Anti-drop scale mark. DETAILED DESCRIPTION

[0032] The specific implementation methods of the utility model are described below to facilitate technical personnel in this technical field to understand the utility model, but it should be clear that the utility model is not limited to the scope of the specific implementation methods. For ordinary technical personnel in this technical field, as long as various changes are within the spirit and scope of the utility model defined and determined by the attached claims, these changes are obvious, and all utility model creations using the concept of the utility model are protected.

[0033] like Figure 1 and 2As shown, the culture container for plant tissue culture includes an upper barrel body 1 and a lower barrel body 2, the opening of the upper barrel body 1 is set with the opening of the lower barrel body 2, and the upper barrel body 1 and the lower barrel body 2 are connected to form a sealed culture cavity. The upper barrel body 1 and the lower barrel body 2 are clearance-matched, and a first sealing ring 3 and a second sealing ring 4 are arranged between the outer wall of the lower barrel body 2 and the inner wall of the upper barrel body 1, and an annular groove for installing the first sealing ring 3 and the second sealing ring 4 is arranged on the lower barrel body 2, and the first sealing ring 3 and the second sealing ring 4 are fixed in the annular groove. The first sealing ring 3 and the second sealing ring 4 are arranged between the upper barrel body 1 and the lower barrel body 2, and the first sealing ring 3 and the second sealing ring 4 can form a secondary seal, thereby improving the overall sealing of the culture container. The upper barrel body 1 is nested on the top of the lower barrel body 2, and the first sealing ring 3 and the second sealing ring 4 can seal the gap between the upper barrel body 1 and the lower barrel body 2, so that the upper barrel body 1 and the lower barrel body 2 are connected to form a culture chamber; when the upper barrel body 1 is completely pulled out from the top of the lower barrel body 2, the upper barrel body 1 is separated from the lower barrel body 2, and the first sealing ring 3 and the second sealing ring 4 are still fixed on the lower barrel body 2. The inner diameter of the first sealing ring 3 and the second sealing ring 4 is interference fit or overfit with the annular groove, and the outer diameter of the first sealing ring 3 and the second sealing ring 4 is interference fit or overfit with the upper barrel body 1. The radius of the annular groove is equal to the cross-sectional radius of the first sealing ring 3, and the depth of the annular groove is equal to the cross-sectional radius of the first sealing ring 3, which facilitates the sealing and fixing of the first sealing ring 3 and the second sealing ring 4 in the annular groove, and at the same time, half of the first sealing ring 3 and the second sealing ring 4 protrude from the surface of the lower barrel body 2, thereby improving the sealing between the upper barrel body 1 and the lower barrel body 2.

[0034] The top of the upper barrel 1 is provided with an air hole 11, and a detachable air permeable membrane 14 is provided in the air hole 11. The air permeable membrane 14 is preferably a PF series tissue culture breathable sealing membrane for isolating bacteria, such as a PF202 tissue culture membrane or a polytetrafluoroethylene microporous film. A breathable cylinder 12 is fixed on the air hole 11, and the breathable cylinder 12 is integrally formed with the upper barrel 1. A limiting ring 13 supporting the bottom of the breathable membrane 14 is provided in the breathable cylinder 12, and a film pressing cylinder 15 for squeezing and supporting the top of the breathable membrane 14 is provided in the breathable cylinder 12, and the inner wall of the breathable cylinder 12 is threadedly connected with the outer wall of the film pressing cylinder 15.

[0035] like Figure 3 and 4 As shown, the film pressing cylinder 15 includes a knob ring and a threaded cylinder matched with the air permeable cylinder 12, the knob ring and the threaded cylinder are integrally formed, and the outside of the knob ring is provided with anti-slip lines. The film pressing cylinder 15 is screwed on the film pressing cylinder 15, so that the bottom of the film pressing cylinder 15 is used to press the air permeable membrane 14, thereby ensuring the sealing between the film pressing cylinder 15 and the air permeable membrane 14, and a rubber ring can be provided at the bottom of the air permeable cylinder 12, and the rubber ring is bonded to the bottom of the air permeable cylinder 12, and the rubber ring can produce a deformation effect, thereby better improving the air tightness between the film pressing cylinder 15 and the air permeable membrane 14.

[0036] The first sealing ring 3 and the second sealing ring 4 have the same structure, and the first sealing ring 3 and the second sealing ring 4 are bonded and fixed in the annular groove. The lower barrel body 2 includes a cylindrical upper sealing portion 21 and a lower conical portion 22, and the upper sealing portion 21 and the lower conical portion 22 are integrally formed, and the first sealing ring 3 and the second sealing ring 4 are arranged on the outer side of the upper sealing portion 21.

[0037] A base 5 is provided at the bottom of the lower barrel body 2, and the radius of the base 5 is greater than the bottom radius of the lower barrel body 2. The base 5 and the base 5 are designed as one piece, and when the upper barrel body 1 is pulled out upward, the base 5 can provide a fixing force in the opposite direction to the lower barrel body 2, thereby facilitating the upper barrel body 1 to be pulled out from the lower barrel body 2, and facilitating tissue culture operations.

[0038] The outer part of the lower barrel 2 is provided with an anti-slip scale mark 6, which is arranged below the second sealing ring 4. When the bottom of the upper barrel 1 is aligned with the second sealing ring 4, the anti-slip scale mark 6 is not blocked by the bottom of the upper barrel 1, and the anti-slip scale mark 6 leaks out. When the staff telescopes the upper barrel 1 upward, they can observe whether the anti-slip scale mark 6 appears, thereby judging whether the lower barrel 1 is telescoped to the maximum stroke, and conveniently adjusting the telescopic height of the upper barrel 1.

[0039] A guide ring 16 is provided on the inner side of the bottom of the upper barrel body 1 , and the upper barrel body 1 is conveniently guided to the lower barrel body 2 through the guide ring 16 , so that the upper barrel body 1 can be easily installed on the lower barrel body 2 .

[0040] Working process and principle: When in use, the breathable membrane 14 is fixed on the limiting ring 13 on the breathable cylinder 12, and then the film pressing cylinder 15 is installed on the breathable cylinder 12. The film pressing cylinder 15 is screwed to press the upper side of the breathable membrane 14 with its bottom, and the breathable membrane 14 is limited and fixed by the cooperation of the limiting ring 13 and the film pressing cylinder 15. During tissue culture operation, the tissue cultured plants are located in the lower barrel body 2, and then the upper barrel body 1 is installed on the lower barrel body 2. The telescopic height of the upper barrel body 1 on the lower barrel body 2 can be adjusted to adapt to tissue culture plants of different heights. By observing the anti-slip scale mark 6 on the lower barrel body 2, it is convenient to observe whether the upper barrel body 1 is telescoped to the maximum height. A secondary seal is formed between the upper barrel body 1 and the lower barrel body 2 through the first sealing ring 3 and the second sealing ring 4, thereby ensuring a sealed and sterile environment of the tissue culture container. At the same time, the breathable membrane 14 arranged on the top of the upper barrel body 1 can allow air to pass through. At the same time, the breathable membrane 14 has a certain antibacterial ability. While ensuring air circulation in the culture cavity, it can also prevent external bacteria from entering the culture cavity, thereby ensuring a sterile environment in the culture cavity. The upper barrel body 1 can be telescopically moved outside the lower barrel body 2 through the first sealing ring 3 and the second sealing ring 4. During the movement, the lower barrel body 2 plays a role similar to a piston. When the upper barrel body 1 reciprocates and telescopes on the lower barrel body 2, the air in the culture chamber can be inhaled and outflowed in cooperation with the air permeable hole 1 and the air permeable membrane 14 on the upper barrel body 1, so that the relative telescopic effect between the upper barrel body 1 and the lower barrel body 2 can be utilized to ventilate the culture chamber.

Claims

1. A culture container for plant tissue culture, characterized in that: It comprises an upper barrel body (1) and a lower barrel body (2), wherein the opening of the upper barrel body (1) and the opening of the lower barrel body (2) are arranged in a sleeve, and the upper barrel body (1) and the lower barrel body (2) are connected to form a sealed culture cavity; A first sealing ring (3) and a second sealing ring (4) are arranged between the outer wall of the lower barrel body (2) and the inner wall of the upper barrel body (1); an annular groove for mounting the first sealing ring (3) and the second sealing ring (4) is arranged on the lower barrel body (2); the first sealing ring (3) and the second sealing ring (4) are fixed in the annular groove; The top of the upper barrel (1) is provided with an air hole (11), and a detachable air permeable membrane (14) is provided in the air hole (11).

2. The culture container for plant tissue culture according to claim 1, characterized in that: A breathable tube (12) is fixed on the breathable hole (11), a limiting ring (13) is arranged inside the breathable tube (12) to support the bottom of the breathable membrane (14), a film pressing tube (15) is arranged inside the breathable tube (12) to squeeze and support the top of the breathable membrane (14), and the inner wall of the breathable tube (12) is threadedly connected to the outer wall of the film pressing tube (15).

3. The culture container for plant tissue culture according to claim 2, characterized in that: The film pressing cylinder (15) comprises a knob ring portion and a threaded cylinder portion cooperating with the air permeable cylinder (12); the knob ring portion and the threaded cylinder portion are integrally formed; and the outside of the knob ring portion is provided with anti-slip patterns.

4. The culture container for plant tissue culture according to claim 1, characterized in that: The first sealing ring (3) and the second sealing ring (4) have the same structure, and the first sealing ring (3) and the second sealing ring (4) are bonded and fixed in the annular groove.

5. The culture container for plant tissue culture according to claim 1, characterized in that: The lower barrel body (2) comprises a cylindrical upper sealing portion (21) and a lower conical portion (22); the upper sealing portion (21) and the lower conical portion (22) are integrally formed; and the first sealing ring (3) and the second sealing ring (4) are arranged on the outer side of the upper sealing portion (21).

6. The culture container for plant tissue culture according to claim 2, characterized in that: The air-permeable cylinder (12) is integrally formed with the upper barrel body (1).

7. The culture container for plant tissue culture according to claim 1, characterized in that: A base (5) is provided at the bottom of the lower barrel body (2), and the radius of the base (5) is greater than the bottom radius of the lower barrel body (2).

8. The culture container for plant tissue culture according to claim 1, characterized in that: The outer portion of the lower barrel (2) is provided with an anti-slip scale mark (6), and the anti-slip scale mark (6) is arranged below the second sealing ring (4).

9. The culture container for plant tissue culture according to claim 1, characterized in that: The upper barrel body (1) and the lower barrel body (2) are clearance-matched.

10. The culture container for plant tissue culture according to claim 1, characterized in that: The radius of the annular groove is equal to the cross-sectional radius of the first sealing ring (3), and the depth of the annular groove is equal to the cross-sectional radius of the first sealing ring (3).

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