A filtration component for mesenchymal stem cell exosomes

Abstract

The utility model relates to the technical field of exosome purification, and particularly discloses a filtering component for mesenchymal stem cell exosomes. A container containing a mixture is placed on a lifting platform, and a knob is rotated to slide the lifting platform upward. When the top of the container abuts against a rubber pad, the rotation can be stopped. After the container abuts against the rubber pad, a closed space is formed with a pressurized barrel, and an opening at the top of a catheter is blocked. The catheter is slid downward to be inserted into the bottom end of a layer of exosomes in the mixture. The opening at the top of the catheter is connected to a container for holding the exosomes, and then an air pump is pushed to inject air into the pressurized barrel. When the air enters the pressurized barrel, the internal pressure of the pressurized barrel increases, and the pressure uniformly applies pressure to the mixture from top to bottom. When the pressure is transmitted to the opening at the bottom end of the catheter, the exosomes are pressed out of the catheter. By applying pressure to the liquid surface of the mixture, the exosomes are filtered out, thereby achieving the effect of improving the filtration purity.

Description

Technical Field

[0001] The utility model relates to the technical field of exosome purification, and in particular to a filtering component for mesenchymal stem cell exosomes. Background Art

[0002] Stem cell exosomes are small vesicles secreted by mesenchymal stem cells during the culture process. They are rich in cholesterol and sphingomyelin, etc. They can mainly regulate cell proliferation and differentiation, and can play a coordinating role in the human body. Various cells and body fluids in the human body can secrete exosomes.

[0003] Existing Chinese patent: A filtering and extracting device for stem cell exosomes, patent number: CN218710404U, including a base, a mixing box is fixedly installed on the left side of the top of the base, a filter box is fixedly installed on the right side of the top of the base, and support rods are fixedly installed on the left and right sides of the top of the mixing box. The utility model drives the stirring rod to rotate by the output end of the rotating motor, and the stirring rod drives the stirring blade to rotate, so as to stir and mix the sample and the diluent in the inner cavity of the mixing box. At this time, the output end of the servo motor drives the screw to rotate, and the screw drives the screw sleeve to move left and right. The screw sleeve drives the fixed box to move left and right through the connecting rod, and the fixed box drives the stirring rod and the stirring blade to move left and right, so as to accelerate the mixing effect of the sample and the diluent. After mixing, the mixed sample is sucked into the inner cavity of the filter box through the input end of the water pump through the conveying pipe. At this time, the sample is filtered through the filter and discharged through the discharge pipe.

[0004] However, during the implementation of the above technical solution, it was found that at least the following technical problems exist: the above-mentioned filtration device uses a filter screen for filtration, which can cause mechanical damage to the exosomes, resulting in low filtration purity. Utility Model Content

[0005] 1. Technical issues to be solved

[0006] In view of the shortcomings of the prior art, the utility model provides a filtration component for mesenchymal stem cell exosomes, which solves the technical problem that the above-mentioned filtration device uses a filter screen for filtration, and the filter screen may cause mechanical damage to the exosomes, resulting in low filtration purity.

[0007] (II) Technical solution

[0008] In order to achieve the above objectives, the present invention is implemented through the following technical solutions:

[0009] A filtering component for mesenchymal stem cell exosomes comprises a support seat, an air pump is fixedly mounted on the top of the support seat, a pressurizing barrel is fixedly mounted on the top of the support seat, a plurality of sealing plugs are threadedly mounted on the top of the pressurizing barrel, a rubber pad is fixedly mounted on the bottom of the pressurizing barrel, a sealing sleeve is threadedly mounted on the top of the pressurizing barrel, a catheter is mounted inside the sealing sleeve, a threaded rod is threadedly mounted inside the support seat, and a lifting platform is rotatably mounted on the top of the threaded rod.

[0010] Preferably: a connecting pipe is fixedly installed on the side end of the pressurizing barrel; the other end of the connecting pipe is connected to the air pump barrel.

[0011] Preferably: a knob is fixedly mounted on the bottom end of the threaded rod.

[0012] (III) Beneficial effects

[0013] 1. First, the mixture is treated by ultracentrifugation of sucrose density gradient centrifugation. The treated solution will be layered according to the density of each component. The container containing the mixture is placed on the lifting platform and the knob is rotated. Since the threaded rod is threadedly connected to the support seat, rotating the knob will cause the lifting platform to slide upward. When the top of the container is against the rubber pad, the rotation can be stopped. After the container is against the rubber pad, it will form a closed space with the pressurized barrel, blocking the opening at the top of the catheter. Slide the catheter downward and insert the catheter into the bottom of a layer of exosomes in the mixture. The opening at the top of the catheter is connected to the container for holding exosomes. Then push the air pump to inject air into the pressurized barrel. When the air enters the pressurized barrel, the internal pressure of the pressurized barrel increases, and the pressure is uniformly applied to the mixture from top to bottom. When the pressure is transmitted to the opening at the bottom of the catheter, the exosomes will be pressed out of the catheter and injected into the container. By applying pressure to the liquid surface of the mixture, the exosomes are filtered out, thereby achieving the effect of improving the filtration purity.

[0014] 2. Unscrew the sealing plug on the top of the pressurized barrel and replace it with a sealing sleeve and a catheter. Insert multiple catheters into the layers of the substances you want to extract, and then inject air into the pressurized barrel through an air pump. Since the pressure will evenly apply pressure to the mixture from top to bottom through the liquid surface, different substances at different levels can be extracted at the same time. By adding multiple sealing sleeves and catheters, the effect of being able to extract multiple substances at the same time is achieved. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The above description is only an overview of the technical solution of the utility model. In order to more clearly understand the technical means of the utility model and implement it according to the contents of the specification, the following is a detailed description of the preferred embodiments of the utility model in conjunction with the accompanying drawings.

[0016] Figure 1 This is a structural diagram of the support seat of the utility model;

[0017] Figure 2 This is a structural diagram of the rubber pad of the utility model;

[0018] Figure 3 This is a cross-sectional structural diagram of the pressurized barrel of the utility model;

[0019] Figure 4 This is a cross-sectional structural diagram of the lifting platform of the utility model.

[0020] Legend: 1. Support seat; 2. Air pump; 3. Threaded rod; 4. Pressurized barrel; 5. Rubber pad; 6. Sealing plug; 7. Sealing sleeve; 8. Conduit; 9. Connecting pipe; 11. Knob; 12. Lifting platform. DETAILED DESCRIPTION

[0021] The embodiment of the present application provides a filtration component for mesenchymal stem cell exosomes, which effectively solves the above-mentioned filtration device. The filter screen is used for filtration, and the filter screen will cause mechanical damage to the exosomes, resulting in a technical problem of low filtration purity. The mixture is first treated by ultracentrifugation sucrose density gradient centrifugation. The treated solution will be layered according to the density of each component. The container containing the mixture is placed on a lifting platform, and the knob is rotated. Since the threaded rod is threadedly connected to the support seat, rotating the knob will cause the lifting platform to slide upward. When the top of the container hits the rubber pad, the rotation can be stopped. After the container hits the rubber pad, it will form an enclosed space with the pressurized barrel, blocking the opening at the top of the catheter. The catheter is slid downward and inserted into the bottom end of a layer of exosomes in the mixture. The opening at the top of the catheter is aligned with the container for holding the exosomes. The device is connected, and then the air pump is pushed to inject air into the pressurized barrel. When the air enters the pressurized barrel, the internal pressure of the pressurized barrel increases, and the pressure applies pressure to the mixture evenly from top to bottom. When the pressure is transmitted to the opening at the bottom of the catheter, the exosomes will be pressed out of the catheter and injected into the container. By applying pressure to the liquid surface of the mixture, the exosomes are filtered out, thereby achieving the effect of improving the filtration purity. The sealing plug on the top of the pressurized barrel is unscrewed and replaced with a sealing sleeve and a catheter. Multiple catheters are respectively inserted into the level of the substances to be extracted, and then air is injected into the pressurized barrel through the air pump. Since the pressure will evenly apply pressure to the mixture from top to bottom through the liquid surface, different substances at different levels can be taken out at the same time. By adding multiple sealing sleeves and catheters, the effect of being able to extract multiple substances at the same time is achieved.

[0022] Example

[0023] like Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, the technical solution in the embodiment of the present application is to effectively solve the technical problem that the above-mentioned filtering device adopts a filter screen for filtering, and the filter screen will cause mechanical damage to the exosomes, resulting in low filtration purity. The overall idea is as follows:

[0024] In response to the problems existing in the prior art, the utility model provides a filtering component for mesenchymal stem cell exosomes, including a support seat 1, an air pump 2 is fixedly installed on the top of the support seat 1, a pressurizing barrel 4 is fixedly installed on the top of the support seat 1, and a plurality of sealing plugs 6 are threadedly installed on the top of the pressurizing barrel 4.

[0025] A rubber pad 5 is fixedly installed on the bottom end of the pressurized barrel 4, a sealing sleeve 7 is threadedly installed on the top end of the pressurized barrel 4, a conduit 8 is installed inside the sealing sleeve 7, and a threaded rod 3 is threadedly installed inside the support seat 1.

[0026] A lifting platform 12 is rotatably mounted on the top of the threaded rod 3 , and a connecting pipe 9 is fixedly mounted on the side end of the pressurized barrel 4 ; the other end of the connecting pipe 9 is connected to the air pump 2 , and a knob 11 is fixedly mounted on the bottom end of the threaded rod 3 .

[0027] Working principle:

[0028] In the first step, the mixture is treated by ultracentrifugation of sucrose density gradient centrifugation. The treated solution will be layered according to the density of each component. The container containing the mixture is placed on the lifting platform 12, and the knob 11 is rotated. Since the threaded rod 3 is threadedly connected to the support seat 1, rotating the knob 11 will cause the lifting platform 12 to slide upward. When the top of the container abuts against the rubber pad 5, the rotation can be stopped. After the container abuts against the rubber pad 5, it will form a closed space with the pressurized barrel 4, blocking the opening at the top of the conduit 8. The conduit 8 is slid downward to guide the conduit 8. The tube 8 is inserted into the bottom end of a layer of exosomes in the mixture, and the opening at the top end of the tube 8 is connected to the container for containing the exosomes. Then the air pump 2 is pushed to inject air into the pressurized barrel 4. When the air enters the pressurized barrel 4, the pressure inside the pressurized barrel 4 increases, and the pressure is uniformly applied to the mixture from top to bottom. When the pressure is transmitted to the opening at the bottom end of the tube 8, the exosomes are pressed out of the tube 8 and injected into the container. By applying pressure to the liquid surface of the mixture, the exosomes are filtered out, thereby achieving the effect of improving the filtration purity.

[0029] In the second step, the sealing plug 6 on the top of the pressurized barrel 4 is unscrewed and replaced with a sealing sleeve 7 and a conduit 8. Multiple conduits 8 are respectively inserted into the layers of the substances to be extracted, and then air is injected into the pressurized barrel 4 through the air pump 2. Since the pressure will evenly apply pressure to the mixture from top to bottom through the liquid surface, different substances at different levels can be extracted at the same time. By adding multiple sealing sleeves 7 and conduits 8, the effect of being able to extract multiple substances at the same time is achieved.

[0030] Finally, it should be noted that: Obviously, the above embodiments are only examples for clearly explaining the present invention, and are not intended to limit the implementation methods. For ordinary technicians in the relevant field, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived from this are still within the scope of protection of the present invention.

Claims

1. A filtration component for mesenchymal stem cell exosomes, comprising a support seat (1), characterized in that The top of the support seat (1) is fixedly mounted with an air pump (2), the top of the support seat (1) is fixedly mounted with a pressurizing barrel (4), the top of the pressurizing barrel (4) is threadedly mounted with a plurality of sealing plugs (6), the bottom of the pressurizing barrel (4) is fixedly mounted with a rubber pad (5), the top of the pressurizing barrel (4) is threadedly mounted with a sealing sleeve (7), the inside of the sealing sleeve (7) is mounted with a conduit (8), the inside of the support seat (1) is threadedly mounted with a threaded rod (3), the top of the threaded rod (3) is rotatably mounted with a lifting platform (12), and the side end of the pressurizing barrel (4) is fixedly mounted with a connecting pipe (9); The other end of the connecting pipe (9) is connected to the air pump (2).

2. The filtration component of mesenchymal stem cell exosomes according to claim 1, characterized in that: A knob (11) is fixedly mounted on the bottom end of the threaded rod (3).

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