A program cooling box for multi-sample cryopreservation tubes

Abstract

The utility model provides a program cooling box for multi-sample cryopreservation tubes, comprising a box body and a cover body arranged in cooperation with the box body, wherein a central hole is provided in the middle of the box body, and a central pillar is provided in cooperation with the lower end of the cover body corresponding to the central hole, so that the central pillar can be inserted into the central hole to improve the sealing effect between the cover body and the box body, and a lifting handle is also provided on the outer end side of the box body and the cover body, and lifting grooves are provided on both sides of the lifting handle for taking the box body and the cover body, so as to facilitate the rapid separation of the box body and the cover body. The cover body is inserted into the central hole of the box body through the central pillar, so that the lower end surface of the cover body and the upper end surface of the box body are sealed in a close fit, thereby improving the sealing effect between the cover body and the box body. The lifting handles on the outer end sides of the box body and the cover body are convenient for personnel to take, and are helpful for quickly separating the cover body. The device improves the cryopreservation effect, reduces the phase change time, and has a better protection effect on cell damage; the amount of cryopreserved samples is large, and multiple samples can be cryopreserved at the same time, and the results are consistent.

Description

Technical Field

[0001] The utility model relates to the technical field of programmed cooling boxes, in particular to a programmed cooling box for multi-sample cryopreservation tubes. Background Art

[0002] Cell culture is one of the most important experimental techniques in the field of life sciences. The quality of cell seeds is a key factor affecting the results of cell culture. Cells are usually stored in liquid nitrogen at -196°C. Prior to this, the cells need to be "slowly frozen" to allow the cells to smoothly pass through the phase transition from liquid to solid. This is programmed cooling. The programmed cooling box is a device used for programmed cooling. The programmed cooling box with cell samples is placed in a -80°C refrigerator. At the storage position of the sample in the box, the sample can be cooled to -80°C at a certain cooling rate (usually 1°C / min).

[0003] There are two main types of program cooling boxes: the first is a polycarbonate plastic box, which needs to be constantly replenished with isopropyl alcohol (isopropyl alcohol consumes costs, is a volatile gas, and is toxic). The temperature characteristics of isopropyl alcohol are used to control the temperature. The second is a polystyrene foam material, which achieves precise temperature control through temperature balance between internal and external materials.

[0004] The existing programmed cooling box has a large overall diameter and is not easy to operate with one hand. Other products either need to be held under the armpit for operation, or the lid is designed to be particularly loose for easy operation, which affects the internal heat conduction. A loose lid will also cause an unstable connection between the two, which can easily cause the lid to be accidentally knocked over, affecting the freezing process. In addition, the existing sample boxes are designed with different hole positions and have three layers of holes, resulting in poor consistency in sample freezing results. Utility Model Content

[0005] The technical problem solved by the utility model is to provide a program cooling box for multi-sample cryopreservation tubes to solve the problems raised in the above-mentioned background technology.

[0006] The technical problem solved by the utility model is achieved by the following technical scheme: a program cooling box for multi-sample cryopreservation tubes, comprising a box body and a cover body arranged in cooperation with the box body, wherein a central hole is opened in the middle of the box body, and a central pillar is arranged in cooperation with the lower end of the cover body corresponding to the central hole, so that the central pillar is inserted into the central hole to improve the sealing effect between the cover body and the box body, and a lifting handle is also arranged on the outer end side of the box body and the cover body, and lifting grooves are arranged on both sides of the lifting handle for taking the box body and the cover body, so as to facilitate the rapid separation of the box body and the cover body;

[0007] The box body is provided with a plurality of test tube holes, which are arranged in a circular distribution to accommodate cell sample tubes. The cover body is provided with cover holes corresponding to the test tube holes to accommodate the tops of the cell sample tubes. A ring-shaped metal module is embedded and installed at the bottom of the center hole of the box body to help conduct heat, realize rapid heat exchange of the cell sample tubes, and improve the freezing effect.

[0008] As a further solution of the utility model:

[0009] The test tube holes are distributed in 24 numbers, and the cover holes are also provided with 24 test tube holes, so that the program cooling box can freeze 24 samples at a time; according to the shape of the cell sample tube, the diameter of the cover hole is larger than the diameter of the test tube hole.

[0010] As a further solution of the utility model:

[0011] The box body and the cover body are composite polystyrene foam structures, and the metal module is a metal alloy structure.

[0012] As a further solution of the utility model:

[0013] A plurality of positioning probes are arranged in a circular arrangement at the lower end of the cover body, and a plurality of positioning holes are arranged on the box body corresponding to the positioning probes. The length of the positioning probes is greater than the height of the center column, so that the positioning probes are extracted and contacted with the box body and inserted into the positioning holes to limit the cover body. The center column can be accurately inserted into the center hole, and the cover hole is stably sleeved on the upper end of the cell sample tube to avoid contact with the cell sample tube when the cover body and the box body are closed, so that the upper end of the cell sample tube is located at the center of the cover hole, and at the same time, the cover body is prevented from squeezing the cell sample tube due to rotation.

[0014] As a further solution of the utility model:

[0015] The cover body is also provided with a locking assembly for fixing the cover body and the box body. The locking assembly includes a pin rod slidably installed on the central column and a rotating shaft member for controlling the telescopic movement of the pin rod. The box body is provided with a pin hole corresponding to the pin rod so that the pin rod can be inserted into the pin hole, thereby locking the cover body to the upper end of the box body.

[0016] As a further solution of the utility model:

[0017] A swivel hole is opened in the middle part of the cover body, and the swivel shaft is inserted into the swivel hole and rotatably connected. The swivel shaft is located at the upper end of the cover body and is provided with a swivel handle to facilitate the rotation of the swivel shaft. The central column is provided with a sliding hole that passes through the swivel hole, and the pin rod is slidably inserted in the sliding hole. One side of the pin rod is inserted into the swivel groove corresponding to the swivel shaft. A guide column is provided at the lower end of the pin rod, and a guide slide groove is provided at the lower end of the swivel groove corresponding to the guide column. The guide slide groove is inserted into the guide slide groove and is slidably arranged. The guide slide groove is an arc-shaped structure. Through the rotation of the swivel shaft, the guide slide groove drives the guide column to move telescopically to pull the pin rod for telescopic adjustment; the pin rod is located at the upper end of the guide column and is a horizontal structure, and is attached to the upper end of the swivel groove to limit the rotation of the pin rod.

[0018] As a further solution of the utility model:

[0019] Both sides of the upper end of the handle are provided with handle plates protruding outwards, so that when a person inserts his fingers into the handle groove, they can be pressed against the handle plates, so that the person can apply force to the handle.

[0020] Compared with the prior art, the beneficial effects of the utility model are as follows: the cover is inserted into the central hole of the box body through the central column, so that the lower end surface of the cover body and the upper end surface of the box body are sealed, thereby improving the sealing effect between the cover body and the box body. The handles on the outer ends of the box body and the cover body are convenient for personnel to take, which helps to quickly disassemble the cover body. The device improves the freezing effect, reduces the phase change time, and has a better protection effect on cell damage; no isopropyl alcohol is used, which reduces the harm to people and the environment; the amount of frozen samples is large, and multiple samples can be frozen at the same time, and the results are consistent.

[0021] A person can rotate the rotary handle to drive the rotary shaft to rotate, and the rotary shaft uses the guide slot to pull the pin rod to extend and retract, so that the pin rod is inserted into the pin hole of the box body, thereby completing the locking and fixing of the cover body and the box body; the device has a simple structure and is not easy to be damaged. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a structural schematic diagram of the utility model;

[0023] Figure 2 The cross-sectional structure of the utility model is shown in FIG. Figure 1 ;

[0024] Figure 3 The cross-sectional structure of the utility model is shown in FIG. Figure 2 ;

[0025] Figure 4 for Figure 3 The enlarged structural diagram at A in the middle;

[0026] Figure 5 This is a schematic diagram of the installation structure of the guide column of the utility model;

[0027] Markings in the figure: 1. box body; 2. cover body; 3. lifting handle; 4. cell sample tube; 5. positioning probe; 6. rotating shaft; 11. center hole; 12. test tube hole; 13. metal module; 21. center column; 22. cover hole; 31. lifting groove; 32. handle plate; 51. positioning hole; 61. pin rod; 62. pin hole; 63. rotating handle; 64. rotating groove; 65. guide column; 66. guide groove. DETAILED DESCRIPTION

[0028] In order to make the technical means for realizing the utility model, creative features, objectives and effects easy to understand, the utility model is further explained below with reference to specific illustrations.

[0029] like Figures 1 to 5 As shown,

[0030] The present embodiment provides a program cooling box for multi-sample cryopreservation tubes, comprising a box body 1 and a cover body 2 arranged in cooperation with the box body 1, wherein a central hole 11 is opened in the middle of the box body 1, and a central pillar 21 is arranged in cooperation with the lower end of the cover body 2 corresponding to the central hole 11, so that the central pillar 21 is inserted into the central hole 11 to improve the sealing effect between the cover body 2 and the box body 1, and a handle 3 is further arranged on the outer end side of the box body 1 and the cover body 2, and lifting grooves 31 are opened on both sides of the handle 3 for taking the box body 1 and the cover body 2, so as to facilitate the rapid separation of the box body 1 and the cover body 2;

[0031] The box body 1 is provided with a plurality of test tube holes 12, which are arranged in a circular distribution to accommodate the cell sample tubes 4. The cover body 2 is provided with cover holes 22 corresponding to the test tube holes 12 to accommodate the tops of the cell sample tubes 4. The box body 1 is embedded with a ring-shaped metal module 13 at the bottom of the central hole 11 to help conduct heat, realize rapid heat exchange of the cell sample tubes 4, and improve the freezing effect.

[0032] Specifically, the personnel place the cell sample tubes 4 in the test tube holes 12 in sequence, and place the cover body 2 on the upper end of the box body 1, insert the central pillar 21 into the central hole 11, and use the cover hole 22 on the cover body 2 to cover the upper end of the cell sample tube 4, and the lower end surface of the cover body 2 is sealed with the upper end surface of the box body 1. The handles 3 on the outer ends of the box body 1 and the cover body 2 are convenient for personnel to take, which helps to quickly separate the cover body 2.

[0033] In this embodiment, 24 test tube holes 12 are distributed, and 24 cover holes 22 are also provided to match the test tube holes 12, so that the program cooling box can freeze 24 samples at a time; according to the shape of the cell sample tube 4, the diameter of the cover hole 22 is larger than the diameter of the test tube hole 12.

[0034] In this embodiment, the box body 1 and the cover body 2 are composite polystyrene foam structures, and the metal module 13 is a metal alloy structure.

[0035] In this embodiment, a plurality of groups of positioning probes 5 are annularly distributed at the lower end of the cover body 2, and a plurality of groups of positioning holes 51 are distributed corresponding to the positioning probes 5 on the box body 1. The length of the positioning probes 5 is greater than the height of the central pillar 21, so that the positioning probes 5 are extracted and contacted with the box body 1, and inserted into the positioning holes 51 to limit the cover body 2. The central pillar 21 can be accurately inserted into the central hole 11, and the cover hole 22 is stably sleeved on the upper end of the cell sample tube 4 to avoid the cell sample tube 4 from touching the cell sample tube 4 when the cover body 2 and the box body 1 are closed, so that the upper end of the cell sample tube 4 is located at the center of the cover hole 22, and at the same time, the cover body 2 is prevented from rotating to squeeze the cell sample tube 4.

[0036] In this embodiment, the cover body 2 is also provided with a locking assembly for fixing the cover body 2 and the box body 1, and the locking assembly includes a pin rod 61 slidably mounted on the central column 21 and a rotating shaft 6 for controlling the telescopic movement of the pin rod 61. A pin hole 62 is provided on the box body 1 corresponding to the pin rod 61, so that the pin rod 61 can be inserted into the pin hole 62, thereby locking the cover body 2 at the upper end of the box body 1.

[0037] A rotary hole is provided in the middle of the cover body 2, and the rotary shaft member 6 is inserted into the rotary hole for rotation connection. The rotary shaft member 6 is located at the upper end of the cover body 2 and is provided with a rotary handle 63 to facilitate the rotation of the rotary shaft member 6. The central column 21 is provided with a sliding hole that passes through the rotary hole, and the pin rod 61 is slidably inserted in the sliding hole. One side of the pin rod 61 is inserted into the rotary groove 64 corresponding to the rotary shaft member 6. A guide column 65 is provided at the lower end of the pin rod 61, and a guide slide groove 66 is provided at the lower end of the rotary groove 64 corresponding to the guide column 65. The guide slide groove 66 is inserted into the guide slide groove 66 for sliding arrangement. The guide slide groove 66 is an arc-shaped structure. Through the rotation of the rotary shaft member 6, the guide slide groove 66 drives the guide column 65 to move telescopically to pull the pin rod 61 for telescopic adjustment; the pin rod 61 is located at the upper end of the guide column 65 and is a horizontal structure, and is attached to the upper end of the rotary groove 64 to limit the rotation of the pin rod 61.

[0038] Specifically, a person can rotate the rotating handle 63 to drive the rotating shaft 6 to rotate, and the rotating shaft 6 uses the guide slot 66 to pull the pin 61 to extend and retract, so that the pin 61 is inserted into the pin hole 62 of the box body 1, and the cover body 2 and the box body 1 are locked and fixed. The pin 61 is inserted into the rotating slot 64, and can abut against the upper and lower sides of the rotating slot 64, limit the up and down movement of the rotating shaft 6, and prevent the rotating shaft 6 from being separated from the rotating hole. The guide column 65 can be elastically retracted and installed on the pin 61. When the pin 61 extends into the rotating slot 64, the guide column 65 elastically extends and is inserted into the guide slot 66, so that the pin 61 and the rotating shaft 6 are stably installed. The device has a simple structure and is not easy to be damaged.

[0039] In this embodiment, handle plates 32 protruding outward are further provided on both sides of the upper end of the handle 3 so that when a person inserts his fingers into the handle groove 31 , they can rest on the handle plates 32 , thereby facilitating the person to apply force to the handle 3 .

[0040] The above shows and describes the basic principle and main features of the utility model and the advantages of the utility model. The technicians in this industry should understand that the utility model is not limited by the above embodiments. The above embodiments and the description only illustrate the principle of the utility model. Without departing from the spirit and scope of the utility model, the utility model will also have various changes and improvements, and these changes and improvements fall within the scope of the utility model to be protected. The scope of protection of the utility model is defined by the attached claims and their equivalents. It should be noted that in this article, if there are first and second relationship terms such as only used to distinguish one entity or operation from another entity or operation, it does not necessarily require or imply that there is any such actual relationship or order between these entities or operations. Moreover, the term "include", "comprise" or any other variant thereof is intended to cover non-exclusive inclusion, so that the process, method, article or equipment including a series of elements includes not only those elements, but also includes other elements that are not explicitly listed, or also includes elements inherent to such process, method, article or equipment. Without more constraints, an element defined by the phrase "comprising a..." does not exclude the existence of other identical elements in the process, method, article or apparatus comprising the element.

Claims

1. A program cooling box for multi-sample cryopreservation tubes, comprising a box body and a cover body arranged in cooperation with the box body, characterized in that: A central hole is provided in the middle of the box body, and a central pillar is provided at the lower end of the cover body corresponding to the central hole, so that the central pillar is inserted into the central hole to improve the sealing effect between the cover body and the box body. A lifting handle is also provided on the outer end side of the box body and the cover body, and lifting grooves are provided on both sides of the lifting handle for taking the box body and the cover body, so as to facilitate the rapid disassembly of the box body and the cover body. The box body is provided with a plurality of test tube holes, which are arranged in a circular shape to accommodate cell sample tubes. The cover body is provided with cover holes corresponding to the test tube holes to accommodate the tops of the cell sample tubes. A ring-shaped metal module is embedded and installed at the bottom of the center hole of the box body to help conduct heat.

2. The program cooling box for multiple sample cryopreservation tubes according to claim 1, characterized in that: The test tube holes are distributed in 24 numbers, and the cover holes are also provided with 24 test tube holes, so that the program cooling box can freeze 24 samples at a time; according to the shape of the cell sample tube, the diameter of the cover hole is larger than the diameter of the test tube hole.

3. The program cooling box for multiple sample cryopreservation tubes according to claim 1, characterized in that: The box body and the cover body are composite polystyrene foam structures, and the metal module is a metal alloy structure.

4. The program cooling box for multiple sample cryopreservation tubes according to claim 1, characterized in that: The lower end of the cover body is provided with a plurality of groups of positioning probes in a circular distribution, and the box body is provided with a plurality of groups of positioning holes corresponding to the positioning probes. The length of the positioning probes is greater than the height of the central column, so that the positioning probes are extracted and contacted with the box body and inserted into the positioning holes to limit the cover body.

5. The program cooling box for multiple sample cryopreservation tubes according to claim 1, characterized in that: The cover body is also provided with a locking assembly for fixing the cover body and the box body. The locking assembly includes a pin rod slidably installed on the central column and a rotating shaft member for controlling the telescopic movement of the pin rod. The box body is provided with a pin hole corresponding to the pin rod so that the pin rod can be inserted into the pin hole, thereby locking the cover body to the upper end of the box body.

6. The program cooling box for multiple sample cryopreservation tubes according to claim 5, characterized in that: A swivel hole is opened in the middle part of the cover body, and the swivel shaft is inserted into the swivel hole and rotatably connected. The swivel shaft is located at the upper end of the cover body and is provided with a swivel handle to control the rotation of the swivel shaft. The central column is provided with a sliding hole that passes through the swivel hole, and the pin rod is slidably inserted in the sliding hole. One side of the pin rod is inserted into the swivel groove corresponding to the swivel shaft. A guide column is provided at the lower end of the pin rod, and a guide slide groove is provided at the lower end of the swivel groove corresponding to the guide column. The guide slide groove is inserted into the guide slide groove and is slidably arranged. The guide slide groove is an arc structure, so that the guide slide groove drives the guide column to move telescopically through the rotation of the swivel shaft, so as to pull the pin rod for telescopic adjustment; the pin rod is located at the upper end of the guide column and is a horizontal structure, and is attached to the upper end of the swivel groove to limit the rotation of the pin rod.

7. The program cooling box for multiple sample cryopreservation tubes according to claim 1, characterized in that: Both sides of the upper end of the handle are provided with handle plates protruding outwards, so that when a person inserts his fingers into the handle groove, they can be pressed against the handle plates, so that the person can apply force to the handle.

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