A low temperature storage device for nano antibodies

Abstract

The utility model discloses a low-temperature storage device for nano antibodies, belonging to the technical field of low-temperature storage devices, comprising an insulated box, wherein a box cover is arranged on the top of the insulated box, and the box cover is fixed to the top of the insulated box by a clamping assembly; a fixing plate is arranged in the inner cavity of the insulated box; the utility model arranges an ice box in the insulated box, so that the inside of the insulated box is kept at a relatively low temperature, so as to perform low-temperature storage on nano antibody test tubes; during the storage process, the test tube is placed in the inner cavity of the positioning tube and the box cover is tightly closed through the coordinated use of an elastic connection assembly and an auxiliary positioning assembly; the test tube can be positioned in the inner cavity of the positioning tube under the coordination of a positioning seat and a clamping seat, so as to prevent the surface of the test tube from being damaged by collision due to bumps during transportation; and after opening the box cover, the test tube can be ejected from the positioning tube by the elastic connection assembly to facilitate the removal of the test tube.

Description

Technical Field

[0001] The utility model relates to the technical field of low-temperature storage devices, in particular to a low-temperature storage device for nano antibodies. Background Art

[0002] Antibodies generally have two identical heavy (H) and light (L) polypeptide chains. Nanobodies have unique properties due to their size and single-chain nature, which can be used in a wide range of applications. Nanobodies have a wide range of applications, mainly in the following aspects: affinity capture reagents, crystallization partners, target imaging and immune modulation, biosensors, in vivo imaging, anti-snake venom therapy, anti-infective drugs, immune-based therapies and bispecific nanobodies. Nanobodies need to be stored at low temperatures during their application.

[0003] The Chinese patent with publication number CN218318154U discloses a nano antibody low-temperature anti-infection storage device, which relates to the field of antibody storage technology, including an incubator, wherein the lower end of the incubator is fixedly connected with a placement frame, and the four corners of the placement frame are fixedly connected with limit strips, and the interior of the incubator is provided with a storage box, and the storage box is clamped in the placement frame, and the interior of the storage box is fixedly connected with a placement tube. The utility model puts the collected sample test tube from the top of the placement tube through the setting of a spring and a clamp, and the clamp is squeezed by the test tube to make the spring shrink back, so that the test tube moves downward until the lower part of the test tube is placed in the limit circle, and then the elasticity of the spring drives the clamp to clamp and fix the upper part of the test tube, effectively preventing the bumps generated during the transportation of the incubator from causing the test tube to collide with the inside of the incubator, causing the test tube to be damaged and unable to detect the sample.

[0004] The nano-antibody cryogenic storage device involved in the above patent uses a clamp to clamp and position the test tube during actual use to prevent the test tube from colliding with the inside of the incubator due to bumps during transportation. However, when taking out the test tube, the force applied by the spring to the surface of the clamp will still act on the surface of the test tube, making it difficult to take the test tube out of the incubator. Therefore, we need to propose a cryogenic storage device for nano-antibodies. Utility Model Content

[0005] The purpose of the utility model is to provide a low-temperature storage device for nano antibodies, which has the advantages of being able to stably store nano antibody test tubes inside an incubator and facilitating the removal of the nano antibody test tubes from the incubator, so as to solve the problems raised in the above-mentioned background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides a low-temperature storage device for nano antibodies, comprising an insulated box, a box cover is arranged on the top of the insulated box, and the box cover is fixed to the top of the insulated box by a snap-on assembly; the inner cavity of the insulated box is provided with a fixing plate, and a plurality of groups of positioning tubes are equidistantly embedded on the surface of the fixing plate, and the bottom of the positioning tube passes through the fixing plate and extends to the interior of the insulated box, and the interior of the positioning tube is provided with a test tube for storing nano antibodies, and an elastic connecting assembly is arranged between the test tube and the positioning tube for facilitating the removal of the test tube from the positioning tube, and the inner cavity of the positioning tube is also installed with an auxiliary positioning assembly that cooperates with the elastic connecting assembly to fix the test tube to the inner cavity of the positioning tube.

[0007] Preferably, the elastic connection assembly includes a positioning seat slidably installed in the inner cavity of the positioning tube, a sliding rod is fixedly installed at the bottom of the positioning seat, the bottom end of the sliding rod passes through the positioning tube and extends to the outside of the positioning tube, a compression spring is fixedly installed at the bottom of the positioning seat and outside the sliding rod, and the bottom end of the compression spring is fixedly connected to the inner wall of the positioning tube.

[0008] Preferably, the auxiliary positioning assembly includes a holder arranged on both sides of the test tube, the inner sides of the two groups of the holders are provided with an arc groove adapted to the test tube, the inner wall of the arc groove fits the surface of the test tube, and the outer sides of the two groups of the holders are fixedly installed with a tension spring, the end of the tension spring away from the holder is fixedly connected to the inner wall of the positioning tube, and the top of the holder is also equipped with a tightening assembly for pressing the holder against the surface of the test tube.

[0009] Preferably, the clamping assembly comprises two groups of clamping blocks arranged above the two groups of clamping seats, and the bottoms of the two groups of clamping blocks are fixedly mounted with connecting plates, and the bottoms of the connecting plates are fixedly connected to the tops of the positioning seats.

[0010] Preferably, the bottom of the inner side of the two groups of the pressing blocks and the top of the outer side of the two groups of the clamping seats are both provided with bevel cuts, and the bevel cuts at the bottom of the inner side of the two groups of the pressing blocks and the bevel cuts at the top of the outer side of the two groups of the clamping seats are slidably connected.

[0011] Preferably, a mounting groove is provided on the inner wall of the positioning tube at a position corresponding to the tension spring, and one end of the tension spring away from the clamping seat is fixedly connected to the inner wall of the mounting groove.

[0012] Preferably, ice boxes for keeping the interior of the insulated box at a low temperature are arranged around the inner cavity of the insulated box, and the ice box is fixed to the inner cavity of the insulated box by a positioning frame, the surface of the positioning frame is in contact with the inner wall of the insulated box, and the bottom of the fixing plate is in contact with the top of the positioning frame.

[0013] Preferably, the snap-fit ​​assembly includes elastic snap-fit ​​parts fixedly mounted on both sides of the box cover, and both sides of the thermal insulation box are provided with snap-fitting grooves adapted to the elastic snap-fit ​​parts, and the snap-fitting portion of the elastic snap-fit ​​parts is snap-fitted to the inner wall of the snap-fitting groove.

[0014] Compared with the prior art, the beneficial effects of the utility model are:

[0015] The utility model can keep the interior of the incubator at a relatively low temperature by arranging an ice box inside the incubator, so as to perform low-temperature storage on the nano antibody test tubes. During the storage process, the test tubes are placed in the inner cavity of the positioning tube and the box cover is tightly closed by using the elastic connection component and the auxiliary positioning component. The test tubes can be positioned in the inner cavity of the positioning tube with the cooperation of the positioning seat and the clamping seat, so as to prevent the surface of the test tubes from being damaged by collision due to bumps during transportation. Moreover, after opening the box cover, the elastic connection component can eject the test tubes from the positioning tube to facilitate the removal of the test tubes. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the structure of the utility model;

[0017] Figure 2 This is a schematic diagram of the structure of the clamping assembly of the utility model;

[0018] Figure 3 This is a schematic diagram of the structure inside the thermal insulation box of the utility model;

[0019] Figure 4 It is a structural schematic diagram of the elastic connection component and the auxiliary positioning component of the utility model;

[0020] Figure 5 For this utility model Figure 4 Enlarged view of point A in the middle.

[0021] In the figure: 1. Insulation box; 2. Box cover; 3. Fixing plate; 4. Positioning tube; 5. Test tube; 6. Positioning seat; 7. Sliding rod; 8. Compression spring; 9. Clamping seat; 10. Arc groove; 11. Tension spring; 12. Tightening block; 13. Connecting plate; 14. Oblique cut; 15. Mounting groove; 16. Ice box; 17. Positioning frame; 18. Elastic clamping piece; 19. Clamping slot. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] See also Figure 1-Figure 5 The utility model provides a low-temperature storage device for nano antibodies, including an incubator 1, the inner cavity of the incubator 1 is provided with a fixing plate 3, the surface of the fixing plate 3 is equidistantly embedded with a plurality of positioning tubes 4, the bottom of the positioning tube 4 passes through the fixing plate 3 and extends to the inside of the incubator 1, the inside of the positioning tube 4 is provided with a test tube 5 for storing nano antibodies, and ice boxes 16 are provided around the inner cavity of the incubator 1, and the ice box 16 is fixed to the inner cavity of the incubator 1 by a positioning frame 17, the surface of the positioning frame 17 is in contact with the inner wall of the incubator 1, and the bottom of the fixing plate 3 is in contact with the top of the positioning frame 17. In this embodiment, the ice box 16 can be fixed along the inner wall of the incubator 1 by the setting of the positioning frame 17, and the nano antibodies in the test tube 5 are refrigerated in the incubator 1 at low temperature by the ice box 16.

[0024] Preferably, a box cover 2 is provided on the top of the insulation box 1, and the box cover 2 is fixed to the top of the insulation box 1 by a clamping assembly. The clamping assembly includes elastic clamps 18 fixedly installed on both sides of the box cover 2. Both sides of the insulation box 1 are provided with clamping grooves 19 adapted to the elastic clamps 18, and the clamping portion of the elastic clamp 18 is clamped with the inner wall of the clamping groove 19.

[0025] By setting the clamping assembly, the box cover 2 can be clamped and fixed on the top of the insulated box 1. Specifically, after the user places the test tube 5 containing the nanoantibody in the positioning tube 4, the box cover 2 can be pressed downward to press the box cover 2 against the top of the insulated box 1. At this time, the clamping parts of the elastic clamping parts 18 on both sides of the box cover 2 will be clamped in the clamping groove 19, thereby clamping the box cover 2 to the top of the insulated box 1.

[0026] Among them, an elastic connection component is arranged between the test tube 5 and the positioning tube 4 to facilitate the test tube 5 to be removed from the positioning tube 4. The elastic connection component includes a positioning seat 6 slidably installed in the inner cavity of the positioning tube 4, and a sliding rod 7 is fixedly installed at the bottom of the positioning seat 6. The bottom end of the sliding rod 7 passes through the positioning tube 4 and extends to the outside of the positioning tube 4. A compression spring 8 is fixedly installed at the bottom of the positioning seat 6 and located outside the sliding rod 7. The bottom end of the compression spring 8 is fixedly connected to the inner wall of the positioning tube 4.

[0027] Through the setting of the elastic connection component, when the box cover 2 is pressed downward, the movement of the box cover 2 will squeeze the top of the test tube 5, so that the test tube 5 presses the positioning seat 6 downward. The movement of the positioning seat 6 will drive the slide bar 7 to move downward and squeeze the compression spring 8. When the user needs to take out the test tube 5, the box cover 2 can be opened. At this time, the reaction force generated by the contraction of the compression spring 8 will push the test tube 5 upward to facilitate the user to take the test tube 5 out of the positioning tube 4.

[0028] It is worth mentioning that the inner cavity of the positioning tube 4 is also installed with an auxiliary positioning component which is used in conjunction with the elastic connecting component to fix the test tube 5 to the inner cavity of the positioning tube 4. The auxiliary positioning component includes a holder 9 arranged on both sides of the test tube 5. The inner sides of the two groups of holders 9 are provided with arc grooves 10 adapted to the test tube 5. The inner walls of the arc grooves 10 fit the surface of the test tube 5. The outer sides of the two groups of holders 9 are fixedly installed with tension springs 11. The end of the tension spring 11 away from the holder 9 is fixedly connected to the inner wall of the positioning tube 4. The top of the holder 9 is also installed with a tightening component for pressing the holder 9 against the surface of the test tube 5. The tightening component includes two groups of tightening blocks 12 arranged above the two groups of holders 9. The bottoms of the two groups of tightening blocks 12 are fixedly installed with connecting plates 13, and the bottom of the connecting plate 13 is fixedly connected to the top of the positioning seat 6.

[0029] Through the setting of the auxiliary positioning component, when the positioning seat 6 moves downward, the connection plate 13 will drive the pressing block 12 to move downward, and the movement of the pressing block 12 will press the holder 9 against the surface of the test tube 5, so that the arc grooves 10 inside the two groups of holders 9 are closely attached to the surface of the test tube 5, so as to play an auxiliary positioning role for the test tube 5, and prevent the test tube 5 from shaking and colliding with the inside of the positioning tube 4 during the transportation of the test tube 5 and being damaged. When taking out the test tube 5, the compression spring 8 will push the positioning seat 6 upward, which will drive the two groups of pressing blocks 12 to move upward. At this time, the reaction force of the tension spring 11 will drive the two groups of holders 9 to move away from the test tube 5, so as to avoid the holder 9 hindering the test tube 5 from being taken out of the positioning tube 4.

[0030] Furthermore, bevel cuts 14 are provided at the bottom of the inner sides of the two groups of clamping blocks 12 and at the tops of the outer sides of the two groups of clamping seats 9, and the bevel cuts 14 at the bottom of the inner sides of the two groups of clamping blocks 12 and the bevel cuts 14 at the tops of the outer sides of the two groups of clamping seats 9 are slidably connected on the surface, and an installation groove 15 is provided on the inner wall of the positioning tube 4 at the corresponding position of the tension spring 11, and one end of the tension spring 11 away from the clamping seat 9 is fixedly connected to the inner wall of the installation groove 15.

[0031] By setting the bevel cut 14, it can be avoided that the bottom of the clamping block 12 contacts the top of the holder 9 when it moves downward. When the clamping block 12 moves downward, the bevel cut 14 at its bottom will align with the bevel cut 14 at the top of the holder 9. Under the guidance of the bevel cut 14, the two groups of clamping blocks 12 will move downward along the outer sides of the two groups of holders 9 and press the holders 9 against the surface of the test tube 5. By setting the mounting groove 15, a flat mounting position can be provided for one end of the tension spring 11.

[0032] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A low temperature storage device for nano antibodies, characterized in that: It comprises an insulation box (1), a box cover (2) is arranged above the insulation box (1), and the box cover (2) is fixed to the top of the insulation box (1) by means of a clamping assembly; The inner cavity of the incubator (1) is provided with a fixing plate (3), and a plurality of groups of positioning tubes (4) are equidistantly embedded on the surface of the fixing plate (3), and the bottom of the positioning tube (4) passes through the fixing plate (3) and extends to the interior of the incubator (1), and a test tube (5) storing the nano-antibody is provided inside the positioning tube (4), and an elastic connection component is provided between the test tube (5) and the positioning tube (4) to facilitate the test tube (5) to be taken out of the positioning tube (4), and the inner cavity of the positioning tube (4) is also installed with an auxiliary positioning component used in conjunction with the elastic connection component to fix the test tube (5) to the inner cavity of the positioning tube (4); The elastic connection assembly comprises a positioning seat (6) slidably mounted in the inner cavity of the positioning tube (4); a sliding rod (7) is fixedly mounted at the bottom of the positioning seat (6); the bottom end of the sliding rod (7) passes through the positioning tube (4) and extends to the outside of the positioning tube (4); a compression spring (8) is fixedly mounted at the bottom of the positioning seat (6) and outside the sliding rod (7); the bottom end of the compression spring (8) is fixedly connected to the inner wall of the positioning tube (4); The auxiliary positioning assembly comprises a holder (9) arranged on both sides of the test tube (5), the inner sides of the two groups of the holders (9) are provided with an arc groove (10) adapted to the test tube (5), the inner wall of the arc groove (10) is in contact with the surface of the test tube (5), the outer sides of the two groups of the holders (9) are fixedly installed with a tension spring (11), the end of the tension spring (11) away from the holder (9) is fixedly connected to the inner wall of the positioning tube (4), and the top of the holder (9) is also provided with a tightening assembly for pressing the holder (9) against the surface of the test tube (5).

2. A low temperature storage device for nanobodies according to claim 1, characterized in that: The clamping assembly comprises two groups of clamping blocks (12) arranged above the two groups of clamping seats (9), and the bottoms of the two groups of clamping blocks (12) are fixedly mounted with connecting plates (13), and the bottoms of the connecting plates (13) are fixedly connected to the tops of the positioning seats (6).

3. A low temperature storage device for nanobodies according to claim 2, characterized in that: The bottom of the inner side of the two groups of the clamping blocks (12) and the top of the outer side of the two groups of the clamping seats (9) are both provided with bevel cuts (14), and the surfaces of the bevel cuts (14) at the bottom of the inner side of the two groups of the clamping blocks (12) and the bevel cuts (14) at the top of the outer side of the two groups of the clamping seats (9) are slidably connected.

4. A low temperature storage device for nanobodies according to claim 1, characterized in that: An installation groove (15) is provided on the inner wall of the positioning tube (4) at a position corresponding to the tension spring (11), and one end of the tension spring (11) away from the holder (9) is fixedly connected to the inner wall of the installation groove (15).

5. A low temperature storage device for nanobodies according to claim 1, characterized in that: Ice boxes (16) are arranged around the inner cavity of the thermal insulation box (1); the ice boxes (16) are fixed to the inner cavity of the thermal insulation box (1) via a positioning frame (17); the surface of the positioning frame (17) is in contact with the inner wall of the thermal insulation box (1); and the bottom of the fixing plate (3) is in contact with the top of the positioning frame (17).

6. A low temperature storage device for nanobodies according to claim 1, characterized in that: The clamping assembly comprises elastic clamping parts (18) fixedly mounted on both sides of the box cover (2); both sides of the thermal insulation box (1) are provided with clamping grooves (19) adapted to the elastic clamping parts (18); the clamping parts of the elastic clamping parts (18) are clamped to the inner walls of the clamping grooves (19).

Images