A molecular diagnostic device

Abstract

The utility model discloses a molecular diagnostic device, and relates to the field of diagnostic devices related to small-sized amplification equipment, especially portable amplification equipment. The molecular diagnostic device provided by the utility model can make the sample liquid or sample processing liquid added into the integrated detection consumable flow between the chambers of the integrated detection consumable through the operation part, so as to obtain the amplification system liquid located in the amplification chamber. The thermal circulation part can amplify and heat the amplification system liquid in the amplification chamber, and the detection part performs fluorescence detection on the amplification system liquid in the amplification to obtain the amplification detection result. The configuration of the amplification system liquid and the amplification detection can be completed through one device, and the integration is high, the number of driving parts is small, the operation is reliable, the detection accuracy is high, and the pollution is small.

Description

Technical Field

[0001] The utility model relates to small-sized amplification equipment, especially the field of diagnostic equipment related to portable amplification equipment, and specifically to a molecular diagnostic device. Background Art

[0002] In vitro diagnostic technology can separate and remove samples such as blood, body fluids, and tissue fluids from organisms, and use in vitro detection reagents, kits, calibrators, quality control materials, etc. to test and verify the samples. The subdivided application scenarios that have been developed include biochemical diagnosis, immunodiagnosis, molecular diagnosis, microbial diagnosis, blood and body fluid diagnosis, and POCT. From the perspective of application scenarios, POCT has independently formed a special subdivided type of equipment with an increasing proportion from the methodological classification. Molecular POCT uses the genetic material of organisms such as DNA or RNA sequence segments as the analysis object, and can achieve timely and accurate detection of low-abundance targets through amplification and enrichment technology in the early stages of invasion of pathogens (viruses, bacteria, microorganisms, etc.). Therefore, this type of equipment can be used for diagnosis in doctors' offices, hospital emergency rooms, workplaces, pharmacies, and even at home, thereby achieving rapid diagnosis of pathogens. Designing molecular POCT diagnostic equipment is the future development direction of precision medicine.

[0003] With the invasion of various types of diseases, especially large-scale epidemic diseases, more and more companies are committed to developing miniaturized, highly integrated integrated diagnostic equipment. Some well-known American companies have designed IDNOW products based on constant temperature amplification technology, including constant temperature amplification products produced by Cue health. However, judging from the development in recent years, the design of primer probes for products corresponding to constant temperature amplification technology is difficult, and the sensitivity of product detection is insufficient. The installed capacity of GeneXpert and FilmArray based on cyclic heating and cooling technology has grown faster in the past two years, which to a certain extent illustrates the stability and high sensitivity of thermal cycling amplification technology. From the design point of view, the size of these two products is relatively large, and the actual installation and use often presents a multi-machine joint use solution, which has high limitations for promotion in primary diagnosis and treatment. Patent publication number CN116328860A and patent announcement number US11679387B2 both disclose an inherited rotary valve solution, and define that the rotary valve is driven to rotate to two different valve positions to connect different chambers and connecting flow channels, thereby driving the fluid to flow in different chambers to complete an integrated extraction and detection solution. This type of equipment has high requirements for the processing accuracy of the rotary valve and the matching accuracy of the combination, and the cost of realization is high and the processing technology is difficult; the integrated detection consumables disclosed in the patent with publication number EP4190890A1 are packaged through a sealing film to form a test kit containing various processing reagents, and the chamber return air flow channel is unobstructed after the sealing film is punctured, and the plug rod is used to drive the liquid to move to different chambers; the solution disclosed in the patent with publication number EP3741839B1 uses two sets of gas sources to drive alternately at different positions, so that the solution to be amplified can alternately shuttle between two temperature zones with different target temperatures to perform amplification, and a fluorescence detection part is configured in the flow channel between the two temperature zones to obtain the amplification results. This solution has very high requirements for the sealing of the consumables and is very demanding on the drive. The precision requirement is very high; the patents with announcement numbers US11904314B2 and US11904315B2 disclose solutions that focus on the need to cover and seal the amplification solution during the amplification process, and then design a wax valve solution that melts the wax valves in different chambers through high-temperature melting to connect the different chambers, and enables the melted wax and the amplified solution to enter the amplification reaction chamber together to achieve a solution that covers the surface of the reaction solution; the patent with announcement number CN117417819A replaces the widely used fluorescence detection with an electrode detection solution. Although the processing cost may be reduced, the system is difficult to achieve multi-target detection. In addition, with the increase in detection requirements, this solution is extremely difficult to meet the detection requirements for obtaining quantitative results. Although various POCT devices have been designed in the prior art, these devices are usually not highly integrated, the system structure is relatively complex, and the processing and assembly precision requirements of the core components are very high. It is very necessary to optimize the integrated detection equipment in a targeted manner.

[0004] How to design a miniaturized diagnostic device with higher integration, fewer driving parts, more reliable operation, higher detection accuracy and less pollution is a technical problem that needs to be solved urgently. Utility Model Content

[0005] The purpose of the utility model is: to address the above-mentioned problems, the utility model provides a molecular diagnostic device, which can be used in conjunction with integrated detection consumables to achieve amplification detection of samples and meet the requirements of high integration, few driving parts, reliable operation, high detection accuracy and low pollution.

[0006] The technical solution adopted by the utility model is as follows:

[0007] A molecular diagnostic device includes an equipment body, on which are provided a plurality of integrated detection consumable receiving parts for receiving integrated detection consumables and performing amplification detection on the integrated detection consumables. The integrated detection consumable receiving parts include a consumable receiving position for carrying the integrated detection consumables, an operating part for allowing the liquid in the integrated detection consumables to flow between the various chambers of the integrated detection consumables, a thermal circulation part for amplifying and heating the integrated detection consumables, and a detection collection unit for detection. The thermal circulation part and the detection collection unit are respectively arranged on opposite sides of the consumable receiving position.

[0008] Furthermore, the chamber of the integrated detection consumables includes a buffer chamber for receiving external liquid, a system chamber for distributing liquid, an amplification chamber for detection, and a liquid separation drive unit for adjusting the air pressure in the amplification chamber. The buffer chamber and the amplification chamber are fluidically connected to the system chamber, and the system chamber is connected to the external environment through a return air port. A flow control valve that changes the on-off relationship is provided between the buffer chamber and the amplification chamber. The liquid separation drive unit can produce elastic deformation under the action of external force and restore the deformation in the absence of external force; the operating unit includes a valve that can change the relationship between the system chamber and the amplification chamber. A clamping part for the on-off relationship between the amplification chambers, a valve control part that can change the on-off relationship of the fluid control valve, and an air source drive part that can connect and adjust the air pressure in the buffer chamber; the valve control part and the clamping part are movably connected to the device body, the valve control part can move to the position of the fluid control valve corresponding to the consumable receiving position and can drive the fluid control valve to open, and can move and move away from the consumable receiving position to close the fluid control valve, the clamping part can move to the position of the liquid separation drive part corresponding to the consumable receiving position and can press the liquid separation drive part to increase the air pressure in the amplification chamber, and can move and move away from the consumable receiving position to reduce the air pressure in the amplification chamber.

[0009] Furthermore, the clamping part includes a pair of clamping units, which are oppositely arranged on both sides of the consumable receiving position. The pair of clamping units can approach each other under the drive of the first driving part and can press the liquid separation driving part from both sides of the thickness direction of the integrated detection consumable.

[0010] Furthermore, the first driving part includes a first driving motor and a transmission screw, the transmission screw is provided with a forward thread segment and a reverse thread segment, and the paired clamping units are respectively threadedly matched with the forward thread segment and the reverse thread segment of the transmission screw.

[0011] Furthermore, the valve control unit is connected to and follows the clamping unit through a transmission block.

[0012] Furthermore, the integrated detection consumables receiving part also includes a hot melt bonding part for destructively sealing the amplification chamber, the front end of the device body is provided with a device air inlet, and the rear end is provided with a device air outlet, the thermal circulation part is provided on the side close to the device air outlet, and the hot melt bonding part and the detection acquisition unit are provided on the side close to the device air inlet.

[0013] Furthermore, the air source driving part includes an air source driving member and an air source connecting pipe, and the buffer chamber is connected to the air source connecting pipe through an air inlet.

[0014] Furthermore, the integrated detection consumables receiving part is provided with a receiving port for receiving integrated detection consumables, and an openable and closable receiving cover is provided at the receiving port. An air source interface is provided on the receiving cover, and one side of the air source interface can be connected with the air source connecting pipe, and the other side can be matched with the air inlet of the integrated detection consumables in the consumables receiving position, and the side of the air source interface that matches the air inlet is provided with a rubber pad for sealing and pressing the air inlet.

[0015] Furthermore, the hot melt bonding part and the detection and collection unit are movably connected to the equipment body through a second driving part; the second driving part includes an alternating transmission mechanism and a second driving motor, and the second driving motor can drive the alternating transmission mechanism to drive the hot melt bonding part and the detection and collection unit away from the consumables receiving position, or drive one of the hot melt bonding part and the detection and collection unit to approach the consumables receiving position and act on the integrated detection consumables in the consumables receiving position.

[0016] Furthermore, a middle substrate is provided in the body of the device, and a consumable receiving position, a thermal circulation part, a detection and collection unit and a hot melt bonding part are arranged on the upper part of the middle substrate, and an air source drive component, a first drive motor and a second drive motor are arranged on the lower part of the middle substrate; the detection part also includes a transceiver detection unit that can perform light wave transmission and light wave reception operations to obtain amplification results, and the transceiver detection unit is connected to the detection and collection unit signal through an optical fiber, and the transceiver detection unit is arranged below the middle substrate.

[0017] In summary, due to the adoption of the above technical solution, the beneficial effects of the utility model are:

[0018] 1. The utility model enables the sample liquid or sample processing liquid added to the integrated detection consumables to flow between the various chambers of the integrated detection consumables through the operating part to obtain the amplification system liquid located in the amplification chamber. The thermal circulation part can amplify and heat the amplification system liquid in the amplification chamber, and the detection part performs fluorescence detection on the amplification system liquid in the amplification to obtain the amplification detection result. The configuration of the amplification system liquid and the amplification detection can be completed through one device, with high integration, few driving parts, reliable operation, high detection accuracy and low pollution.

[0019] 2. The clamping part of the utility model increases the air pressure in the amplification chamber when pressing the liquid separation driving part, thereby preventing the liquid in the system chamber from entering the amplification chamber. When the liquid separation driving part is released, the liquid separation driving unit is deformed and restored to reduce the air pressure in the amplification chamber to form a suction effect, so that the liquid in the system chamber can be sucked into the amplification chamber.

[0020] 3. The valve control part of the utility model can follow the clamping part to achieve that when the buffer chamber and the system chamber are connected, the amplification chamber and the system chamber are closed; when the buffer chamber and the system chamber are closed, the amplification chamber and the system chamber are connected.

[0021] 4. The air input into the device body through the air inlet of the utility model can flow through the first drive motor, the second drive motor and the hot melt joint in time, so that the waste heat in the device body can be effectively and promptly brought out of the device body through the cooling fan of the heat circulation part.

[0022] 5. The utility model can realize simultaneous or separate detection of multiple integrated detection consumables.

[0023] 6. The clamping part of the utility model acts on the liquid separation driving part by pressing on both sides. Compared with the solution of pressing on one side, pressing on both sides makes the required deformation amount of the coating of the liquid separation driving part smaller when pressed, and the adjustable range of pressure is larger.

[0024] 7. The hot-melt bonding part of the utility model can seal the amplification chamber and reliably ensure the pressure in the amplification chamber. It can form a low-pressure leakage or even a pressure-free leakage state similar to a pressure cooker. In this way, the amplification system liquid can avoid the generation of bubbles during high and low temperature cycle amplification, and the destructive sealing operation also puts multiple amplification chambers in an independent closed state, with almost no problems of steam generation and mutual interference.

[0025] 8. The utility model adopts an air source driving part and a clamping part to realize dual driving source drive of the integrated detection consumables. The air source driving part and the clamping part cooperate with each other and serve as the power source for liquid transfer between different chambers in different time periods. Different power sources can also adapt to the differentiated requirements of different driving forces and driving accuracy, which can ensure that the entire detection method is implemented at low cost and high efficiency, and the risk of mutual interference is relatively small.

[0026] 9. The detection and collection unit and the thermal circulation part of the utility model are arranged on two opposite sides of the consumables receiving position so that when the thermal circulation part contacts the amplification chamber on one side for heat transfer, the detection and collection unit can press the amplification chamber on the other side, so that the central detection hole of the detection and collection unit can fit the amplification chamber more closely, reducing adverse effects such as dispersion.

[0027] 10. The detection and collection unit and the transceiver detection unit of the utility model are connected via optical fiber to reduce light transmission loss and avoid interference risks such as crosstalk. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a schematic diagram of a sample tube that can be used in conjunction with the utility model;

[0029] Figure 2 It is a structural schematic diagram of the utility model integrated detection consumables;

[0030] Figure 3 It is a schematic diagram of the structure of the molecular diagnostic equipment of the utility model;

[0031] Figure 4 This is a schematic diagram of the structure of the molecular diagnostic equipment receiving integrated detection consumables of the utility model;

[0032] Figure 5 It is a state diagram of the utility model undertaking a single integrated testing consumable;

[0033] Figure 6 It is a state diagram of another perspective of the utility model taking on a single integrated detection consumable;

[0034] Figure 7 This is a state diagram of two consumable receiving positions of the utility model receiving two integrated detection consumables;

[0035] Figure 8 It is a side sectional view of the integrated detection consumable of the utility model;

[0036] Fig. 9 It is a side sectional view of the utility model in which the valve control part and the clamping part are synchronously driven to act on the integrated detection consumables;

[0037] Fig.10 It is a structural schematic diagram of the first driving motor shared by the valve control part and the clamping part of the present invention;

[0038] Fig.11 It is a schematic diagram of the structure of the utility model in which the valve control part and the clamping part act together on the integrated detection consumables;

[0039] Fig.12 It is a schematic diagram of the structure in which the air source driving member of the utility model is connected to the buffer chamber in the open state of the flow control valve;

[0040] Fig.13 It is a structural diagram of the layout of the gas source drive component and the first drive motor of the utility model;

[0041] Fig.14 It is a schematic diagram of the state in which the gas source driving member drives the liquid to flow between the system cavity and the buffer cavity when the flow control valve of the utility model is in the open state;

[0042] Fig.15 It is a state diagram of the liquid stored in the cavity of the utility model system;

[0043] Fig.16 It is a state diagram of the liquid in the cavity of the utility model system being driven by the negative pressure of the liquid dispensing driving part and distributed to the amplification cavity;

[0044] Fig.17 This is a state diagram of the second driving motor of the utility model driving the hot melt bonding part to close the amplification cavity;

[0045] Fig.18 This is a state diagram of the utility model where the hot melt bonding part is away from the integrated detection consumable after the fusion is completed;

[0046] Fig.19 It is a cross-sectional view of the detection part of the utility model pressing the amplification chamber and cooperating with the thermal cycle part to complete the amplification detection;

[0047] Fig. 20 It is a structural schematic diagram of the transceiver detection unit of the utility model;

[0048] Fig.21 It is a structural schematic diagram of the mobile bracket of the utility model;

[0049] Fig. 22 This is a state diagram of the utility model in which the alternating transmission mechanism drives the hot melt bonding part to extend;

[0050] Fig.23 It is a state diagram of the utility model in which the alternating transmission mechanism drives the hot melt bonding part and the detection and collection part to be spaced apart from the consumable receiving position;

[0051] Fig.24 This is a state diagram of the utility model in which the alternating transmission mechanism drives the detection and collection part to extend.

[0052] Markings in the figure: 2-device body, 10-integrated detection consumables, 101-box cover, 111-air inlet, 112-air return port, 12-buffer chamber, 123-fluid control valve, 13-system chamber, 14-amplification chamber, 15-liquid separation drive unit, 161-combination auxiliary unit, 17-first flow channel, 18-second flow channel, 30-receiving cover, 301-snap buckle, 31-gas source interface, 32-box cover accommodating part, 100-sample tube, 200-middle substrate, 2001, 2002-integrated detection consumables receiving part, 201-consumables receiving position, 202-control unit, 203-equipment air inlet, 21-valve control unit, 211-magnet, 22-alternating transmission mechanism, 221-second drive motor, 222-movable bracket, 23- Thermal circulation part, 231-cooling fan, 232-fin cooling unit, 233-heating part, 234-heat pipe heat exchanger, 24-clamping part, 241-first driving motor, 242-transmission screw, 243-transmission guide rail, 25-air source driving part, 26-detection and collection unit, 261-contact part, 2611-optical fiber, 27-hot melt bonding part, 28-multi-channel transceiver end, 280-optical fiber fixing hole, 108-first rotating rod, 109-second rotating rod, 110-first connecting rod, 120-second connecting rod, 11-mounting plate, 1071-movable hole of pressing part, 1072-movable hole of sealing part, 1101-first connecting part, 1102-second connecting part, 1201-third connecting part, 1202-fourth connecting part. DETAILED DESCRIPTION

[0053] The utility model is described in detail below in conjunction with the accompanying drawings.

[0054] In order to make the purpose, technical solution and advantages of the utility model more clear, the utility model is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the utility model and are not used to limit the utility model.

[0055] Example 1

[0056] Reference Figure 1 It is a schematic diagram of a sample tube 100 that can be used in conjunction with the utility model. The sample tube 100 here can contain a sample preservative solution so that the collected sample solution can be preserved for a long time. The sample here can be various types of swabs such as nasal swabs and throat swabs. In other cases, it can also be secretions such as saliva or blood, tissue fluid, etc. Of course, the sample tube 100 can also contain a lysis solution, so that the sample can be fully lysed through human action and can be directly added to the integrated detection consumables 10. Of course, in a special case, the sample tube 100 may not be included and the sample can be directly added to the integrated detection consumables 10.

[0057] The integrated detection consumables 10 provided by the utility model are as follows Figure 2 As shown, the integrated detection consumable 10 can be adapted to molecular diagnostic equipment, which is also called a small molecular diagnostic POCT device. The integrated detection consumable 10 includes different chambers. In actual operation, in order to smoothly drive the liquid to be accurately transferred between different chambers without leakage risk, the integrated detection consumable 10 is vertically inserted into the molecular diagnostic equipment, so that different components in the molecular diagnostic equipment can act on the integrated detection consumable 10 to make the liquid therein flow in different chambers according to a preset path. A sample addition port is arranged in the upper area of ​​the integrated detection consumable 10 in the vertical direction. Here, in order to ensure the stability of the reagents inside the integrated detection consumable 10 and the requirements of closed operation, the sample addition port is detachably connected to the box cover 101, and the two can be connected in a detachable manner such as snap-fitting or threading. In order to meet the needs of convenience, low cost, etc. in production and processing, the integrated detection consumables 10 are equipped with a buffer chamber 12 and a system chamber 13, which are connected by a transfer flow channel, and a flow control valve 123 is arranged at at least one position of the transfer flow channel, so as to achieve the goal of changing the connection relationship between the buffer chamber 12 and the system chamber 13 by changing the opening of the flow control valve 123. In order to ensure that the buffer chamber 12 and the system chamber 13 have a large volume and can transfer the liquid therein without residue, the two are formed into a basically identical structure, both having a columnar receiving part with a uniform cross-section at the top and a conical guide part with a gradually decreasing cross-section from top to bottom at the bottom, one end of the transfer flow channel is connected to the guide part at the bottom of the buffer chamber 12, and the other end is connected to the guide part at the bottom of the system chamber 13, and a distribution flow channel is also connected to the guide part at the bottom of the system chamber 13, and the connection port of the distribution flow channel corresponding to the system chamber 13 is arranged at intervals from the connection port of the transfer flow channel corresponding to the system chamber 13.

[0058] A plurality of amplification chambers 14 are arranged in the middle area of ​​the integrated detection consumable 10. The specific number can be set according to the demand. In order to ensure the balance of flow resistance, the amplification chambers 14 here can be optimally configured as an even number. The amplification chamber 14 is optimally configured as a shape with a variable cross-sectional area, including a droplet shape or a droplet-like shape, an egg shape, an ellipsoid, a cone-like shape or other rotating bodies. This can ensure that the liquid is fully developed during the filling process, so that the turbulence is smaller and the probability of generating air gaps is smaller. Finally, the filling degree in the amplification chamber 14 is higher, and a filling degree of 95%-100% can be achieved. The subsequent amplification is more reliable and the detection accuracy is higher. A liquid separation drive unit 15 is arranged at the lower part of the integrated detection consumable 10. In order to ensure that the liquid separation drive unit 15 can apply a uniform driving force to the liquid filling in the several amplification chambers 14, the liquid separation drive unit 15 can include liquid separation drive units corresponding to the number of amplification chambers 14. In this embodiment, the amplification chamber 14 and the liquid-dispensing driving unit in the liquid-dispensing driving part 15 are configured as a hollow through-cavity structure formed on the consumable substrate of the integrated detection consumable 10, and then a deformable and recoverable coating is attached to the two opposite sides in the thickness direction. The deformable and recoverable coating here can be made of materials such as propylene, polyethylene, polystyrene, cycloolefin copolymer (COC), polyester film, polyacetate, or even leather material or rubber material. The deformable and recoverable coating can also be a metal film that meets the needs. Of course, the deformable and recoverable coating here can also be a composite film composed of multiple materials; the coating of the deformable and recoverable film here optimally has a transmittance of 80%-95%. In order to take into account the requirements of film strength, transmittance and thermal conductivity, a coating with an optimal thickness of 0.02mm-0.12mm is selected. In the closed integrated detection consumable 10, the amplification chamber 14 can directly contact and transfer heat with the thermal circulation part 23 through the coating, so configuring a coating with an optimal thickness can ensure the highest heat transfer efficiency. The liquid separation driving chamber and the attached coating are combined to form a liquid separation driving unit, and the clamping part 24 can press at least a part of the coating corresponding to the liquid separation driving unit, and cause the coating at the clamping part 24 to deform. After the clamping part 24 releases the pressure on the liquid separation driving unit, the deformed coating forms a suction effect during the deformation recovery process, so that the liquid to be distributed in the system cavity 13 is filled into the amplification chamber 14. In this way, there is no need to independently configure a deformation recovery part to serve as the driving force for filling the amplification chamber 14, and the coating with appropriate thickness itself has sufficient strength and deformation recovery ability, which makes the design of the entire integrated detection consumable 10 simpler, and avoids the problem that the transmittance is affected by the excessively thick coating and the detection quality is affected. In this embodiment, a plurality of hollow combining auxiliary parts 161 are also arranged between the amplification chamber 14 and the liquid separation driving part 15.

[0059] Figure 3 It is a schematic diagram of the structure of the molecular diagnostic equipment of the utility model. Figure 4The structure of the molecular diagnostic equipment receiving the integrated detection consumables 10 is schematically shown; the molecular diagnostic equipment includes an equipment body 2, on which an integrated detection consumables receiving part 2001 and an integrated detection consumables receiving part 2002 are provided, the integrated detection consumables receiving part 2001 and the integrated detection consumables receiving part 2002 have the same structure, and both can receive the integrated detection consumables 10, a control part 202 is arranged at the front of the molecular diagnostic equipment, the control part 202 includes an interface that can interact with the user, so that the molecular diagnostic equipment can be independently operated by the user, and an equipment air inlet 203 is arranged below the control part 202. In the embodiment, the integrated detection consumable 10 is in a closed state, so the air inlet 203 of the device can provide a flowing heat dissipation airflow source for each module in the molecular diagnostic equipment. Here, the integrated detection consumable receiving part 2001 and the integrated detection consumable receiving part 2002 both include a matching receiving cover 30, and the receiving cover 30 can be snap-fitted and connected with the device body 2, and a box cover accommodating part 32 and an air source interface 31 are also configured on the receiving cover 30. The box cover accommodating part 32 is used to accommodate the box cover 101 on the integrated detection consumable 10 when the receiving cover 30 is snap-fitted on the device body 2; the air source interface 31 can be fluidically connected to the air source The driving member 25, where the air source interface 31 is matched with the integrated detection consumable 10, is also provided with a rubber pad on one side. When the receiving cover 30 is buckled to the device body 2 through the buckle 301, the air source interface 31 can seal and press the air inlet 111 of the integrated detection consumable 10 through the rubber pad. The air inlet 111 is connected with the buffer cavity 12, so that the air source driving member 25 can be fluidically connected with the integrated detection consumable 10, and the system cavity 13 is connected with the external environment through the air return port 112. The system cavity 13 can inhale or discharge air through the air return port 112. When the liquid is transferred in the system cavity 13, the air flow balance in the system cavity 13 is maintained. In this embodiment, the integrated detection consumables receiving part 2001 and the integrated detection consumables receiving part 2002 can operate independently, and can simultaneously receive two integrated detection consumables 10 to perform efficient simultaneous detection, or can separately receive two integrated detection consumables 10, so that while one integrated detection consumable 10 is performing detection, another integrated detection consumable 10 can be added to the device body 2. For example, if the integrated detection consumables receiving part 2001 is performing detection operations, the integrated detection consumables receiving part 2002 that is not operating can receive the integrated detection consumables 10 that arrives, thereby adapting to the scenario requirements of on-demand detection.

[0060] Figure 5 This is a state diagram of a single integrated testing consumable 10 of the utility model. Figure 6It is a state diagram of the utility model from another perspective of a single integrated detection consumable 10; the integrated detection consumable receiving part 2001 and the integrated detection consumable receiving part 2002 are both configured with a consumable receiving position 201 that can independently receive the integrated detection consumable 10. After the integrated detection consumable 10 is correctly configured in one of the integrated detection consumable receiving part 2001 and the integrated detection consumable receiving part 2002, the integrated detection consumable 10 can be snapped into the consumable receiving position 201. Here, in order to ensure that the integrated detection consumable 10 can be reliably snapped, the consumable receiving position 201 includes two opposing vertical card slots. In this embodiment, the consumable receiving position 201 is configured with a thermal circulation part 23 on one side along the thickness direction of the integrated detection consumable 10, and a detection collection unit 26 and a hot melt bonding part 27 on the other side; The thermal circulation part 23 includes a cooling fan 231, a fin cooling unit 232, a heating part 233 and a heat pipe heat exchanger 234; the cooling fan 231 is matched with the fin cooling unit 232; the heating part 233 is matched with the consumable receiving position 201. When the integrated detection consumable 10 is received in the consumable receiving position 201, the heating surface of the heating part 233 can contact the amplification cavity 14 of the integrated detection consumable 10, so as to realize efficient heat transfer heating of the liquid to be amplified in the amplification cavity 14. More specifically, the heating surface of the heating part 233 can directly contact and act on the corresponding coating of the amplification cavity 14; the heat pipe heat exchanger 234 is arranged between the fin cooling unit 232 and the heating part 233, and the cold end of the heat pipe heat exchanger 234 is arranged through the fin cooling unit 232, and the hot end is connected in the heating part 233. A detection collection unit 26 and a hot melt bonding part 27 are arranged on the other side of the integrated detection consumable 10 in the thickness direction. The hot melt bonding part 27 and the detection collection unit 26 are movably connected to the device body 2 through the second driving part. The second driving motor 221 can drive the alternating transmission mechanism 22 to drive the hot melt bonding part 27 and the detection collection unit 26 away from the integrated detection consumable 10, or drive one of the hot melt bonding part 27 and the detection collection unit 26 to approach and act on the integrated detection consumable 10. Fig.21As shown, the alternating transmission mechanism 22 includes a first rotating rod 108, a second rotating rod 109, a first connecting rod 110 and a second connecting rod 120, a movable bracket 222, a column 1011, a mounting plate 11, a first connecting portion 1101, a second connecting portion 1102, a third connecting portion 1201 and a fourth connecting portion 1202, the mounting plate 11 is fixed on the middle base plate 200, and the mounting plate 11 is provided with a column 1011, and the column 1011 is distributed on the movable bracket 22 2 on both sides, the first connecting rod 110 passes through the movable bracket 222 and is connected to the first connecting holes on the two side columns 1011, the second connecting rod 120 passes through the movable bracket 222 and is connected to the second connecting holes on the two side columns 1011, the movable bracket 222 is provided with a pressing part movable hole 1071 and a sealing part movable hole 1072, the second rotating rod 109 passes through the pressing part movable hole 1071, the first rotating rod 108 passes through the sealing part movable hole 1072, and the two sides of the movable bracket 222 The structure is symmetrical. On the first side of the movable bracket 222, the first connecting rod 110 is connected to the first rotating rod 108 through a first connecting portion 1101. One side of the first connecting portion 1101 is hinged to the first connecting rod 110 and the other side is hinged to the first rotating rod 108. The first rotating rod 108 is connected to the hot melt bonding portion 27 through a second connecting portion 1102. One side of the second connecting portion 1102 is hinged to the first rotating rod 108 and the other side is hinged to the hot melt bonding portion 27. The second connecting rod 120 The second rotating rod 109 is connected to the second rotating rod 109 through the third connecting part 1201, one side of the third connecting part 1201 is hinged to the second connecting rod 120, and the other side is hinged to the second rotating rod 109. The second rotating rod 109 is connected to the detection and collection unit 26 through the fourth connecting part 1202, one side of the fourth connecting part 1202 is hinged to the second rotating rod 109, and the other side is hinged to the detection and collection unit 26. When the second driving motor 221 drives the mobile bracket 222 to move upward in the vertical direction, as shown in FIG. Fig. 22 As shown, the first rotating rod 108 and the second rotating rod 109 move upward with the movable bracket 222, and the first rotating rod 108 moves horizontally along the movable hole 1071 of the pressing part, so that the third connecting part 1201 and the fourth connecting part 1202 form an upward bending angle, thereby driving the detection and collection unit 26 to retreat in the horizontal direction away from the consumable receiving position 201, and at the same time, the second rotating rod 109 moves horizontally along the movable hole 1072 of the sealing part, so that the first connecting part 1101 and the second connecting part 1102 form a horizontal straight line structure, thereby driving the hot melt bonding part 27 to extend in the horizontal direction toward the consumable receiving position 201; when the second driving motor 221 drives the movable bracket 222 to move downward in the vertical direction, as shown in FIG. Fig.23As shown, the first rotating rod 108 and the second rotating rod 109 move downward with the movable bracket 222, and the first rotating rod 108 moves horizontally along the movable hole 1071 of the pressing part, so that the angle of the upward bending angle formed by the third connecting part 1201 and the fourth connecting part 1202 gradually increases, thereby driving the detection and collection unit 26 to extend toward the consumable receiving position 201 in the horizontal direction, and at the same time, the second rotating rod 109 moves horizontally along the movable hole 1072 of the sealing part, so that the first connecting part 1101 and the second connecting part 1102 form a downward bending angle, thereby driving the hot melt bonding part 27 to retreat in the horizontal direction away from the consumable receiving position 201. At this time, the detection and collection unit 26 and the hot melt bonding part 27 are both spaced from the consumable receiving position 201. As the second driving motor 221 drives the movable bracket 222 to continue to move downward in the vertical direction, as shown Fig.24 As shown, a horizontal straight line structure is formed between the third connecting part 1201 and the fourth connecting part 1202, thereby driving the detection collection unit 26 to press the integrated detection consumable 10 in the consumable receiving position 201, and at the same time, the second rotating rod 109 moves horizontally along the movable hole 1072 of the sealing part, so that the downward bending angle formed by the first connecting part 1101 and the second connecting part 1102 is reduced, thereby driving the hot melt bonding part 27 to retreat in the horizontal direction away from the consumable receiving position 201.

[0061] In the actual configuration, the detection and collection unit 26 and the hot melt bonding part 27 are arranged at the equipment air inlet 203 which is closer to the front end of the molecular diagnostic equipment, while the thermal circulation part 23 is arranged at the rear end of the molecular diagnostic equipment close to the equipment air outlet. In this way, the air input into the molecular diagnostic equipment through the equipment air inlet 203 can flow through the second drive motor 221 and the hot melt bonding part 27 in time, so that the waste heat in the molecular diagnostic equipment can be effectively and timely removed from the molecular diagnostic equipment through the action of the cooling fan 231.

[0062] Figure 7 It is a state diagram of the two consumable receiving positions 201 of the utility model receiving two integrated detection consumables 10; in this configuration, the two integrated detection consumable receiving parts 2001 and the integrated detection consumable receiving parts 2002 are both configured with integrated detection consumables 10, and in this embodiment, the two integrated detection consumables 10 can be snapped into the corresponding consumable receiving positions 201, and the integrated detection consumable receiving parts 2001 and the integrated detection consumable receiving parts 2002 are both equipped with independent thermal circulation parts 23, detection collection units 26 and hot melt bonding parts 27, so that the integrated detection consumables 10 in the two consumable receiving positions 201 can be detected simultaneously or separately.

[0063] Figure 8 It is a side sectional view of the integrated detection consumable 10 of the utility model. Fig. 92 is a side sectional view of the valve control part 21 and the clamping part 24 being synchronously driven to act on the integrated detection consumable 10; in this embodiment, a transfer channel that can connect the buffer chamber 12 and the system chamber 13 is arranged near the top of the integrated detection consumable 10, and a flow control valve 123 that can open or close the transfer channel is arranged at the transfer channel, and a liquid separation drive part 15 is arranged at the bottom of the integrated detection consumable 10, and the flow control valve 123 is a magnetic control valve. When the valve control part 21 is not close to the flow control valve 123, the flow control valve 123 is in a normally closed state, closing the buffer chamber 12 and the system chamber 13. transfer channel; when the magnet 211 on the valve control part 21 is close to the flow control valve 123, the flow control valve 123 can release the seal of the transfer channel between the buffer chamber 12 and the system chamber 13 under the magnetic attraction of the magnet 211, so that the transfer channel is opened, so that the gas source driving part 25 can be connected to the buffer chamber 12 and change the air pressure in the buffer chamber 12. When the air pressure in the buffer chamber 12 increases, the liquid can flow from the buffer chamber 12 through the opened transfer channel into the system chamber 13. When the air pressure in the buffer chamber 12 decreases, the liquid can flow from the system chamber 13 into the buffer chamber 12 through the opened transfer channel. The clamping part 24 can apply a clamping force to the liquid separation driving part 15, so that the pressure in the amplification chamber 14 fluidically connected to the liquid separation driving part 15 increases, which can limit the liquid from the system chamber 13 to flow into the amplification chamber 14, and also ensure the reliability of the gas source driving part 25 in driving the liquid flow process, effectively reducing the risk of contamination.

[0064] Fig.10 This is a schematic diagram of the structure of the valve control part 21 and the clamping part 24 of the utility model, which share the first driving motor 241. Fig.11It is a schematic diagram of the structure of the utility model in which the valve control part 21 and the clamping part 24 work together on the integrated detection consumables 10; in the process of the gas source driving part 25 driving the liquid to transfer between the buffer chamber 12 and the system chamber 13, it is necessary to limit the liquid from the system chamber 13 to flow into the amplification chamber 14. Since the amplification chamber 14 is fluidically connected to the liquid separation driving part 15, the amplification chamber 14 can be placed in a positive pressure state through the liquid separation driving part 15, thereby forming a resistance to limit the flow of liquid between the amplification chamber 14 and the system chamber 13. In order to achieve the reliability of system operation, in this embodiment, the valve control part 21 and the clamping part 24 share the first drive motor 241, the whole system has a higher degree of integration, low control difficulty, and can ensure that the connection relationship between the buffer chamber 12 and the system chamber 13, and the amplification chamber 14 and the system chamber 13 can be synchronously regulated. In order to ensure that the clamping part 24 can be driven with high precision, the output of the first drive motor 241 is connected to the transmission screw 242 through a coupling. Here, the transmission screw 242 is a double-threaded transmission screw including a forward-spinning thread segment and a reverse-spinning thread segment. Here, in order to ensure that the clamping part 24 can more reliably apply a clamping force to the liquid separation drive part 15, the clamping part 24 includes a pair of clamping units, and the pairs of clamping units are opposed to each other and are respectively threadedly engaged with different thread segments of the transmission screw 242. When the first drive motor 241 rotates, the pairs of clamping units can be relatively close to each other, thereby pressing the liquid separation drive part 15 from both sides in the thickness direction of the integrated detection consumable 10. Compared with the single-sided pressing scheme, the double-sided pressing makes the required deformation of the coating of the liquid separation drive part 15 smaller when being pressed, and the range of adjustable pressure is larger. In order to ensure that each amplification chamber 14 has basically the same driving pressure, the liquid separation drive part 15 includes multiple liquid separation drive units. Accordingly, the clamping unit includes a connecting part and a clamping element corresponding to the liquid separation drive unit one by one. One end of the connecting part is threadedly connected to the transmission screw 242, and the other end is connected to the clamping element. The clamping element protrudes from the connecting part. The valve control part 21 is connected to the clamping unit on the corresponding side through the transmission block and follows the movement. When the first driving motor 241 drives the clamping part 24 to clamp the liquid separation driving part 15, the valve control part 21 is synchronously close to the flow control valve 123, so that the flow control valve 123 is opened, and the two actions of connecting the buffer chamber 12 and the system chamber 13 and closing the amplification chamber 14 and the system chamber 13 are carried out simultaneously; when the first driving motor 241 drives the clamping part 24 to loosen the liquid separation driving part 15, the valve control part 21 is synchronously away from the flow control valve 123, so that the flow control valve 123 is closed, and the two actions of closing the buffer chamber 12 and the system chamber 13 and connecting the amplification chamber 14 and the system chamber 13 are carried out simultaneously. In order to ensure the stable and reliable characteristics of the transmission operation, a transmission guide rail 243 parallel to the length direction of the transmission screw 242 is also provided, and the connecting part is movably connected to the transmission guide rail 243, and the transmission guide rail 243 provides support force for the connecting part.

[0065] Fig.12It is a structural schematic diagram of the air source driving member 25 connected to the buffer chamber 12 when the flow control valve 123 is in the open state; when driven by the common first driving motor 241, the flow control valve 123 is in the open state, and the air source driving member 25 can be connected to the buffer chamber 12 of the integrated detection consumable 10 through the air source interface 31 on the snap-fitting receiving cover 30. Here, the air source driving member 25 can be a plunger pump type driving member, which changes the air pressure state in the buffer chamber 12 through the suction action of the plunger, thereby transferring the liquid between the buffer chamber 12 and the system chamber 13. At the same time, since the clamping part 24 presses the liquid separation driving part 15, it can limit the liquid in the system chamber 13 from entering the amplification chamber 14. Of course, the air source driving member 25 can output the drive to make the liquid flow between the buffer chamber 12 and the system chamber 13 several times to achieve sufficient mixing of various reagents and samples.

[0066] Fig.13 It is a layout structure diagram of the air source drive 25 and the first drive motor 241; in order to ensure the rationality of the system configuration, a middle substrate 200 is configured in the molecular diagnostic equipment, and a consumable receiving position 201 and a thermal circulation part 23, a detection and collection unit 26 and a hot melt bonding part 27 distributed on both sides thereof are arranged on the upper part of the middle substrate 200, and two air source drives 25, two first drive motors 241, and two second drive motors 221 are arranged on the lower part of the middle substrate 200. Such a centralized arrangement of the motion drive unit can minimize the noise during operation and make the maintenance of the molecular diagnostic equipment simpler. In one case, the two air source drives 25 are arranged in the center and the two first drive motors 241 are arranged on both sides of the two air source drives 25, so that the risk of resonance during the operation of the whole machine is smaller.

[0067] Fig.14 It is a schematic diagram of the state in which the gas source driving component 25 drives the liquid to flow between the system chamber 13 and the buffer chamber 12 when the flow control valve is open; in this embodiment, the reagent in the system chamber 13 is configured in a freeze-dried state, so the liquid is driven to flow between the buffer chamber 12 and the system chamber 13 several times, which can fully dissolve the freeze-dried reagent to form a system liquid with higher sensitivity.

[0068] Fig.15 is a state diagram of the liquid stored in the system cavity 13, Fig.16It is a state diagram in which the liquid in the system cavity 13 is driven by the negative pressure of the liquid separation driving part 15 to be distributed to the amplification cavity 14; the mixed liquid, i.e., the system liquid, can be driven by the gas source driving part 25 to be stored in the system cavity 13. At this time, the first driving motor 241 can output a rotational drive to make the two opposite clamping units of the clamping part 24 move away from each other, and the valve control part 21 can gradually move away from the integrated detection consumable 10. The film of the liquid separation driving part 15 can gradually deform and recover to generate negative pressure in the amplification cavity 14. The system liquid in the system cavity 13 can be driven to flow into the amplification cavity 14 and mix the reagents in the amplification cavity 14 to form an amplification liquid. In this step, the valve control part 21 in the away state can release the magnetic attraction of the flow control valve 123 to seal the transfer channel between the buffer cavity 12 and the system cavity 13. In this way, the integrated detection consumable 10 can achieve timely valve sealing and minimize the risk of contamination.

[0069] Fig.17 1 is a state diagram of the second driving motor 221 driving the hot melt bonding part 27 to close the amplification chamber 14; the amplification chamber 14 can be fluidically connected to the corresponding liquid dispensing driving unit through the first flow channel 17 corresponding to the amplification chamber 14, and is fluidically connected to the distribution flow channel through the second flow channel 18 corresponding to the amplification chamber 14. Here, the second driving motor 221 can drive the movable bracket 222 to move upward, and then drive the hot melt bonding part 27 to approach and contact the integrated detection consumable 10 through the alternating transmission mechanism 22. Here, the hot melt bonding part 27 can output a contact temperature of 180°C-240°C, so that the coating is thermally fused to the consumable substrate, thereby destructively closing the first flow channel 17 and The second flow channel 18 makes the amplification chamber 14 in a sealed state. If the temperature here is too low, it will cause a poor seal, which will lead to the risk of leakage, while if the temperature is too high, it may cause excessive thermal shrinkage of the coating or even partial carbonization of the coating, which will lead to the problem of thermal fusion failure. In order to improve the reliability and success rate of fusion, the consumable matrix is ​​also provided with a combining auxiliary part 161, and the first flow channel 17 or the second flow channel 18 is arranged between the two combining auxiliary parts 161, so that the first flow channel 17 and the second flow channel 18 corresponding to each amplification chamber 14 can be separated by intervals, and the combining auxiliary part 161 can bear the thermal fusion deformation, so that the stress and strain in the fusion process can be released in time, thereby ensuring the reliability of the fusion bonding. Fig.18 In order to complete the state diagram of the hot melt bond 27 being away from the integrated detection consumable 10 after fusion, the second driving motor 221 can drive the movable bracket 222 to move downward, driving the detection acquisition unit 26 to approach and contact the integrated detection consumable 10, and the away hot melt bond 27 makes the closed integrated detection consumable 10 unaffected, and can make the waste heat of the hot melt bond 27 be quickly discharged. In order to ensure the reliability of the closure, the hot melt bond 27 includes triangular, conical and other configuration parts with gradually decreasing cross-sections close to the side of the integrated detection consumable 10, so that the hot melt bond 27 can quickly and reliably close the amplification chamber 14 in a smaller operating area.

[0070] Fig.19 It is a cross-sectional view of the detection and collection unit 26 pressing the amplification chamber 14 and cooperating with the thermal circulation part 23 to complete the amplification detection; the second driving motor 221 can drive the detection and collection unit 26 to contact the integrated detection consumable 10, and the thermal circulation part 23 and the detection and collection unit 26 are respectively arranged on both sides of the thickness direction of the integrated detection consumable 10, and the detection and collection unit 26 includes a raised contact part 261, and the contact part 261 is configured as a spherical or quasi-spherical contact head, which can contact and press the amplification chamber 14 of the integrated detection consumable 10 from one side in the thickness direction, and the thermal circulation part 23 on the other side can more closely contact the coating of the amplification chamber 14, improve the heat transfer conditions, and make the central detection hole located at the center of the contact part 261 fit the amplification chamber 14 more closely, reduce the adverse effects such as dispersion, and the detection and collection unit 26 and the transceiver detection unit are connected through the optical fiber 2611 to reduce the light transmission loss and avoid the interference risk such as crosstalk, combined with Fig. 20 The transceiver detection unit includes a plurality of transmitting units formed by optical fibers 2611, a plurality of receiving units formed by optical fibers 2611, and a multi-channel transceiver end 28. One end of the transmitting unit and the receiving unit is connected to the detection and collection unit 26, and the other end is connected to the corresponding optical fiber fixing hole 280 on the transceiver connection plate. The plurality of optical fiber fixing holes 280 are arranged in a line along the length direction of the transceiver connection plate. The multi-channel transceiver end 28 can move back and forth along the length direction of the transceiver connection plate under the drive of the transceiver drive motor, and then the multi-channel transceiver end 28 can move through and scan the plurality of through optical fiber fixing holes 280 arranged in a line, and complete the operation of transmitting and receiving light waves to obtain the amplification results in the amplification chamber 14. The transceiver drive motor can drive the multi-channel transceiver end 28 to move by means of pulley drive, sprocket drive, etc. Fig. 20 As shown in FIG. 1 , a belt wheel transmission method is used to drive the multi-channel transceiver 28 to move and perform a scanning operation.

[0071] The principles and implementation methods of the present invention are described in this article using specific embodiments. The description of the above embodiments is only used to help understand the method and core idea of ​​the present invention. It should be pointed out that for ordinary technicians in this technical field, the present invention can also be improved and modified without departing from the principle of the present invention, and these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A molecular diagnostic device, characterized in that: The device comprises a device body, on which are provided a plurality of integrated detection consumable receiving parts for receiving integrated detection consumables and performing amplification detection on the integrated detection consumables. The integrated detection consumable receiving parts comprise a consumable receiving position for carrying the integrated detection consumables, an operating part for allowing the liquid in the integrated detection consumables to flow between the various chambers of the integrated detection consumables, a thermal circulation part for amplifying and heating the integrated detection consumables, and a detection part for detection. The detection and acquisition units of the thermal circulation part and the detection part are respectively arranged on opposite sides of the consumable receiving position.

2. The molecular diagnostic device according to claim 1, wherein The chamber of the integrated detection consumables includes a buffer chamber for receiving external liquid, a system chamber for distributing liquid, an amplification chamber for detection, and a liquid separation drive unit for adjusting the air pressure in the amplification chamber. The buffer chamber and the amplification chamber are both fluidically connected to the system chamber, and the system chamber is connected to the external environment through a return air port. A flow control valve for changing the on-off relationship is provided between the buffer chamber and the amplification chamber. The liquid separation drive unit can produce elastic deformation under the action of external force and restore the deformation without the action of external force; the operating unit includes a valve that can change the relationship between the system chamber and the amplification chamber. A clamping part that can establish an on-off relationship between the consumables and the fluid control valve, a valve control part that can change the on-off relationship of the fluid control valve, and an air source drive part that can connect and adjust the air pressure in the buffer chamber; the valve control part and the clamping part are movably connected to the device body, the valve control part can move to the position of the fluid control valve corresponding to the consumables receiving position and can drive the fluid control valve to open, and can move and move away from the consumables receiving position to close the fluid control valve, the clamping part can move to the position of the liquid separation drive part corresponding to the consumables receiving position and can press the liquid separation drive part to increase the air pressure in the amplification chamber, and can move and move away from the consumables receiving position to reduce the air pressure in the amplification chamber.

3. The molecular diagnostic device according to claim 2, wherein: The clamping part includes a pair of clamping units, which are oppositely arranged on both sides of the consumable receiving position. The pair of clamping units can approach each other under the drive of the first driving part and can press the liquid separation driving part from both sides of the thickness direction of the integrated detection consumable.

4. The molecular diagnostic device according to claim 3, characterized in that The first driving part comprises a first driving motor and a transmission screw, the transmission screw is provided with a forward thread segment and a reverse thread segment, and the paired clamping units are respectively threadedly matched with the forward thread segment and the reverse thread segment of the transmission screw.

5. The molecular diagnostic device according to claim 4, characterized in that The valve control part is connected with the clamping unit through a transmission block and follows the clamping unit.

6. The molecular diagnostic device according to claim 4, characterized in that The integrated detection consumables receiving part also includes a hot melt bonding part for destructively sealing the amplification chamber. The front end of the device body is provided with a device air inlet, and the rear end is provided with a device air outlet. The thermal circulation part is arranged on the side close to the device air outlet, and the hot melt bonding part and the detection collection unit are arranged on the side close to the device air inlet.

7. The molecular diagnostic device according to claim 4, characterized in that The air source driving part comprises an air source driving member and an air source connecting pipe, and the buffer chamber is communicated with the air source connecting pipe through an air inlet.

8. The molecular diagnostic device according to claim 7, wherein: The integrated detection consumables receiving part is provided with a receiving port for receiving integrated detection consumables, and an openable and closable receiving cover is provided at the receiving port. The receiving cover is provided with an air source interface, one side of the air source interface can be connected with the air source connecting pipe, and the other side can be matched with the air inlet of the integrated detection consumables in the consumables receiving position, and the side of the air source interface that matches the air inlet is provided with a rubber pad for sealing and pressing the air inlet.

9. The molecular diagnostic device according to claim 6, wherein: The hot melt bonding part and the detection and collection unit are movably connected to the device body through a second driving part; the second driving part includes an alternating transmission mechanism and a second driving motor, and the second driving motor can drive the alternating transmission mechanism to drive the hot melt bonding part and the detection and collection unit away from the consumables receiving position, or drive one of the hot melt bonding part and the detection and collection unit to approach the consumables receiving position and act on the integrated detection consumables in the consumables receiving position.

10. The molecular diagnostic device according to claim 9, characterized in that A middle substrate is arranged in the body of the device, and a consumables receiving position, a thermal circulation part, a detection and collection unit and a hot melt bonding part are arranged on the upper part of the middle substrate, and an air source drive component, a first drive motor and a second drive motor are arranged on the lower part of the middle substrate; the detection part also includes a transceiver detection unit that can perform light wave transmission and light wave reception operations to obtain amplification results, and the transceiver detection unit is connected to the detection and collection unit signal through an optical fiber, and the transceiver detection unit is arranged below the middle substrate.

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