A cell culture roller bottle

Abstract

The utility model discloses a cell culture roller bottle, which relates to the technical field of roller bottles, and specifically includes a bottle body, a bottle mouth is provided on the top of the bottle body, a bottle cap is provided on the bottle mouth, a mounting port is provided in the middle of the bottle cap, a breathable membrane is provided in the mounting port, a mounting ring is provided in the bottle mouth, and the two are threadedly connected; a leakproof conical cover is coaxially installed at one end of the mounting ring away from the bottle mouth opening, and a vent hole is provided at the tip of the leakproof conical cover, and the vent hole is coaxial with the bottle body. When the utility model is in use, firstly, the culture solution is blocked by the leakproof conical cover and will not leak, so that the roller bottle can be safely rolled on the roller bottle machine without leakage, and at this time, the mounting port on the bottle cap can be opened larger to facilitate more air from the outside to enter the bottle; secondly, the bottle body and the bottle mouth are both straight cylindrical, so as to avoid the problem that the bottle mouth is narrow and it is inconvenient to take out the cells, and it is also convenient to pour the culture solution into it, which is simple to operate and easy to use.

Description

Technical Field

[0001] The utility model relates to the technical field of roller bottles, in particular to a cell culture roller bottle. Background Art

[0002] The cell culture roller bottle is a kind of container for cell culture, which is mainly used in laboratory cell research and industrial production of biological products, including recombinant proteins, monoclonal antibodies, viral vaccines and other cell-produced biological materials. In the actual operation process, the roller bottle will be placed on the roller bottle machine, the general speed is 10-20r / h, the liquid volume is 10%, and as the bottle rotates, the cells in the liquid stick to the wall, so that the cells cultured in the roller bottle do not have to be immersed in the culture medium all the time, which is conducive to cell respiration and material exchange.

[0003] Existing roller bottles are divided into wide mouth (such as Figure 6 as shown) and narrow openings (as Figure 5 As shown) Figure 5 As shown in FIG, the narrow-mouth roller bottle has a small mouth, which makes it inconvenient to pour culture medium and culture cells; Figure 6 As shown, when the wide-mouth roller bottle is rolled, the culture solution inside it will flow to the bottle mouth and the bottle cap, causing cells to grow on the bottle cap. To this end, we propose a cell culture roller bottle. Utility Model Content

[0004] In view of the defect that the culture fluid in the existing roller bottle easily flows into the bottle cap and the bottle mouth, the utility model provides a cell culture roller bottle, which has the advantage that the bottle mouth can be blocked by a leak-proof conical cover to prevent the culture fluid from flowing into the bottle cap, thereby solving the problems raised in the above-mentioned background technology.

[0005] In order to achieve the above objectives, the present invention is implemented through the following technical solutions:

[0006] A cell culture roller bottle is designed, comprising a bottle body, a bottle mouth is arranged on the top of the bottle body, a bottle cap is arranged on the bottle mouth, a mounting opening is arranged in the middle of the bottle cap, a breathable membrane is arranged in the mounting opening, a mounting ring is arranged in the bottle mouth, and the two are threadedly connected;

[0007] A leak-proof cone cover is coaxially mounted on one end of the mounting ring away from the bottle mouth opening. A vent hole is provided at the tip of the leak-proof cone cover, and the vent hole is coaxial with the bottle body.

[0008] Preferably, a bracket is connected to the inner wall of the mounting ring, and the bracket is in a straight line or a cross shape.

[0009] Preferably, the bottle body includes a bottle body, a first annular seat, and a second annular seat, the first annular seat is installed at the bottom of the bottle body, the second annular seat is installed at the top of the bottle body, the bottle mouth is arranged on the top opening of the bottle body, and the height of the first annular seat and the second annular seat is higher than the height of the bottle body.

[0010] Preferably, a step is provided between the bottle mouth and the bottle body so that an annular groove is formed between the bottle mouth and the bottle body, the mounting ring is threadedly connected in the annular groove, and a sealing ring is provided at the bottom of the step to abut against the end of the mounting ring.

[0011] Preferably, the bottle mouth and the bottle cap are threadedly connected, an annular seat is provided in the bottom of the bottle cap, the annular seat is located outside the mounting port, an annular cavity for accommodating a sealing ring is provided between the annular seat and the side wall of the bottle mouth, the bottle mouth is placed in the annular cavity and abuts against the sealing ring.

[0012] Preferably, scale lines are provided on the surface of the bottle body.

[0013] Preferably, the inner surface of the bottle body is provided with wrinkles.

[0014] Compared with the prior art, when the utility model is used, firstly, the culture liquid is blocked by the leakproof conical cover and will not leak, so that the roller bottle can be safely rolled on the roller bottle machine without leakage. At this time, the installation opening on the bottle cap can be opened larger to facilitate more external air to enter the bottle;

[0015] Secondly, the bottle body and the bottle mouth are both straight-cylindrical, which avoids the problem of the bottle mouth being narrow and inconvenient to take out the cells, and also makes it convenient to pour the culture medium into it. It is simple to operate and easy to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of the utility model Figure 1 .

[0017] Figure 2 This is a schematic diagram of the structure of the utility model Figure 2 .

[0018] Figure 3 It is a cross-sectional view of the utility model.

[0019] Figure 4 It is a structural schematic diagram of the bottle cap of the utility model.

[0020] Figure 5 Schematic diagram of a narrow-mouth roller bottle.

[0021] Figure 6 Schematic diagram of a wide-mouth roller bottle.

[0022] In the figure: 1. bottle body; 2. first annular seat; 3. second annular seat; 4. bottle mouth; 5. mounting ring; 6. bottle cap; 7. mounting port; 8. bracket; 9. breathable membrane; 10. leak-proof conical cover; 11. annular groove; 12. annular seat; 13. air vent. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] See also Figures 1 to 4 The utility model provides a technical solution: a cell culture roller bottle, comprising a bottle body, the bottle body is transparent, and comprises a bottle body 1, a bottle mouth 4 is provided on the top of the bottle body 1, and the bottle mouth 4 is threadedly connected with a bottle cap 6;

[0025] The bottom of the bottle body 1 is coaxially provided with a first annular seat 2, and the other end is coaxially provided with a second annular seat 3. The bottle mouth 4 is arranged on the top opening of the bottle body 1. Figure 1 and Figure 3 As shown, the heights of the first annular seat 2 and the second annular seat 3 are higher than the heights of the bottle body 1 and the bottle cap 6;

[0026] In actual use, the operation process of the rolling bottle is to place the rolling bottle on the rolling bottle machine, control the rotation speed between 5-20r / h, and the amount of culture fluid is generally about 10% of the volume of the rolling bottle. As the bottle rotates, the cells in the culture fluid stick to the wall, so that the cells cultured in the rolling bottle do not have to be immersed in the culture fluid all the time, which is conducive to cell respiration and material exchange. Therefore, the rolling bottle rotates continuously when in use. If the entire side of the bottle body contacts the rolling bottle machine, the contact surface between the two is too large, which is easy to cause wear on the side of the bottle body. If the bottle body is worn, it will affect the operator's direct observation of its internal situation through the bottle body. Therefore, this solution allows the first annular seat 2 and the second annular seat 3 to be supported on the rolling bottle machine, thereby avoiding the contact between the side of the bottle body 1 and the rolling bottle machine.

[0027] like Figure 2 As shown, a mounting opening 7 for ventilation is provided in the middle of the bottle cap 6, and a breathable membrane 9 is provided inside the mounting opening 7. The breathable membrane 9 is used to prevent external debris from entering the bottle, and the external air enters the bottle body after being filtered through the breathable membrane 9.

[0028] Most importantly, a blocking mechanism is provided in the bottle mouth 4 to prevent the culture solution from flowing into the bottle cap 6, that is, a mounting ring 5 is provided in the bottle mouth 4, and the bottle mouth 4 and the mounting ring 5 are threadedly connected;

[0029] The inner wall of the mounting ring 5 is connected with a bracket 8, which is in a straight line or cross shape. The bracket 8 is arranged to facilitate the operator to rotate the mounting ring 5 by hand, thereby facilitating the tightening and loosening of the mounting ring 5. Figure 3As shown, a step is provided between the bottle mouth 4 and the bottle body 1, so that an annular groove 11 is formed between the bottle mouth 4 and the bottle body 1, and the mounting ring 5 is threadedly connected in the annular groove 11. A sealing ring is provided at the bottom of the step to abut against the end of the mounting ring 5. When the mounting ring 5 is gradually advanced, the mounting ring 5 is tightly abutted against the sealing ring, thereby achieving a better sealing state;

[0030] like Figure 3 As shown, a leak-proof conical cover 10 is coaxially mounted on one end of the mounting ring 5 away from the bottle mouth, and a vent hole 13 is provided at the tip of the leak-proof conical cover 10. The vent hole 13 is coaxial with the bottle body, and the leak-proof conical cover 10 extends toward the bottle body 1 or toward the inside of the mounting ring 5. The culture medium ( Figure 3 The culture fluid will be blocked by the leakproof conical cover 10 and thus confined inside the bottle body 1. The advantages of this are that, firstly, the culture fluid is prevented from flowing into the bottle mouth, thereby allowing cells to grow on the bottle cap; secondly, it is convenient for operators to directly observe everything inside the bottle body 1, and it will not be difficult to observe because the cells enter the bottle cap.

[0031] Further, such as Figure 4 As shown, an annular seat 12 is provided at the bottom of the bottle cap 6, and the annular seat 12 is located outside the installation port 7. An annular cavity for accommodating a sealing ring is provided between the annular seat 12 and the side wall of the bottle mouth 4. The bottle mouth 4 is placed in the annular cavity and abuts against the sealing ring to achieve a better sealing effect.

[0032] Furthermore, the surface of the bottle body 1 is provided with scale lines to facilitate direct observation of the amount of culture solution added, and the inner surface of the bottle body 1 is provided with folds to facilitate the cells to adhere to the inner surface of the bottle body 1.

[0033] Finally, it should be noted that, since the culture solution in the bottle body 1 is blocked by the leakproof conical cover 10 and will not leak, the mounting opening 7 on the bottle cap 6 can be larger than the air permeation opening of the existing breathable membrane bottle cap, so as to allow as much external air as possible to enter the bottle;

[0034] At the same time, the bottle body 1 and the bottle mouth 4 are both straight-cylindrical, which avoids the problem that the bottle mouth 4 is narrow and inconvenient to take out the cells, and is also convenient for pouring the culture solution therein, and is simple to operate and easy to use.

[0035] In the description of the present utility model, it should be noted that the orientation or position relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside", etc. is based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation of the present utility model; the terms "first", "second", and "third" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance. In addition, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances. Moreover, the terms "comprises," "comprising," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that includes a list of elements includes not only those elements but also other elements not expressly listed, or also includes elements inherent to such process, method, article, or apparatus.

[0036] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell culture roller bottle, comprising a bottle body, a bottle mouth (4) is provided on the top of the bottle body, and a bottle cap (6) is provided on the bottle mouth (4), characterized in that: A mounting opening (7) is provided in the middle of the bottle cap (6), a breathable membrane (9) is provided in the mounting opening (7), and a mounting ring (5) is provided in the bottle mouth (4), and the two are threadedly connected; A leakproof conical cover (10) is coaxially mounted on one end of the mounting ring (5) away from the bottle mouth opening. A vent hole (13) is provided at the tip of the leakproof conical cover (10), and the vent hole (13) is coaxial with the bottle body.

2. A cell culture roller bottle according to claim 1, characterized in that: A bracket (8) is connected to the inner wall of the mounting ring (5), and the bracket (8) is in a straight line shape or a cross shape.

3. A cell culture roller bottle according to claim 1, characterized in that: The bottle body comprises a bottle body (1), a first annular seat (2), and a second annular seat (3); the first annular seat (2) is mounted on the bottom of the bottle body (1), the second annular seat (3) is mounted on the top of the bottle body (1), a bottle mouth (4) is arranged on the top opening of the bottle body (1), and the heights of the first annular seat (2) and the second annular seat (3) are higher than the height of the bottle body (1).

4. A cell culture roller bottle according to claim 3, characterized in that: A step is provided between the bottle mouth (4) and the bottle body (1), so that an annular groove (11) is formed between the bottle mouth (4) and the bottle body (1), the mounting ring (5) is threadedly connected in the annular groove (11), and a sealing ring is provided at the bottom of the step to abut against the end of the mounting ring (5).

5. A cell culture roller bottle according to claim 1, characterized in that: The bottle mouth (4) and the bottle cap (6) are threadedly connected. An annular seat (12) is provided in the bottom of the bottle cap (6). The annular seat (12) is located outside the mounting opening (7). An annular cavity for accommodating a sealing ring is provided between the annular seat (12) and the side wall of the bottle mouth (4). The bottle mouth (4) is placed in the annular cavity and abuts against the sealing ring.

6. A cell culture roller bottle according to claim 4, characterized in that: The surface of the bottle body (1) is provided with scale lines.

7. A cell culture roller bottle according to claim 1, characterized in that: The inner surface of the bottle body (1) is provided with wrinkles.

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