A device for detecting exosome protein concentration

Abstract

The utility model discloses an exosome protein concentration detection device, including: a stirring mechanism, a vacuum mechanism, and a detection mechanism; the characteristics are: the stirring mechanism: a sample tank, a motor, and a stirring fan blade; the vacuum mechanism includes: a plurality of sample temporary storage balls, a vacuum pump, and a sample temporary storage tube; the plurality of sample temporary storage balls are distributed along a circumferential array, and connecting tubes are provided between adjacent sample temporary storage balls, and the connecting tubes are respectively fixedly connected to adjacent sample temporary storage balls; the detection mechanism includes: a sample storage pool, a plurality of sample detection blocks, and an electrochemical sensor; a second delivery tube is provided on the sample detection block, and the two ends of the second delivery tube are respectively fixedly connected to the sample detection block and the sample temporary storage ball. The utility model is provided with a vacuum mechanism, and a low-pressure environment is created by a vacuum pump, so that the sample solution is more easily sucked into the sample temporary storage ball; the design of the vacuum mechanism of the utility model accelerates the sample transmission process and improves the detection efficiency.

Description

Technical Field

[0001] The utility model relates to the technical field of exosome protein concentration detection, and in particular to an exosome protein concentration detection device. Background Art

[0002] Exosome proteins are a type of extracellular vesicles (EVs), which are tiny membrane-bound vesicles that are actively secreted by cells. The composition of exosome proteins reflects their intracellular origin, and in different types of cells and body fluids, exosomes contain the same marker molecules, such as Alix, TSG101, SNARE, RAB GTPase, and four transmembrane proteins CD9, CD63, CD81, etc.

[0003] At present, the prior art CN 212301398 U discloses an exosome protein concentration detection device, including a magnetic-electrochemical sensor box, a sample storage pool is movably plugged into the front side of the magnetic-electrochemical sensor box, a connecting tube is provided on the front surface of the sample storage pool, a sample temporary storage tube is plugged into the interior of the connecting tube, an on-off switch 1 is fixedly installed on the upper part of the sample temporary storage tube, a connecting seat is fixedly connected to the top of the sample temporary storage tube, and a horizontal reagent tube is fixedly connected to the top of the connecting seat. The connection between the horizontal reagent tube and the sample temporary storage tubes of each part is controlled by sliding a piston. When each sample temporary storage tube is filled with exosomes to be detected, the piston is pushed back to the on-off switch 2. At this time, all the on-off switches 1 are opened at the same time, so that the exosomes entering the sample tank for detection can enter at the same time, thereby achieving the effect of adding samples in the same amount at the same time to ensure the detection accuracy.

[0004] The above device may have the following problems when in use:

[0005] 1. Low detection accuracy. Exosome proteins are tiny vesicles, and the actual exosome concentration detection requires very little sample solution. When the solution enters the pipeline, due to the influence of the liquid surface tension, solution retention and solution residue will appear on the connecting tube, making it impossible to add samples at the same time, which in turn affects the detection accuracy.

[0006] 2. The detection speed is slow; when a small amount of exosome solution passes through the horizontal reagent tube and enters the sample storage tube, it is affected by the dual effects of gravity and liquid surface tension, causing the exosomes to flow slowly in the pipe.

[0007] Therefore, it is necessary to provide an exosome protein concentration detection device to solve the above technical problems. Summary of the invention

[0008] The utility model overcomes the shortcomings of the prior art and provides a device for detecting the concentration of exosome protein.

[0009] In order to achieve the above-mentioned purpose, the technical solution adopted by the utility model is: an exosome protein concentration detection device, comprising: a stirring mechanism, a vacuum mechanism, and a detection mechanism; characterized in that:

[0010] The stirring mechanism comprises a sample tank, a motor arranged on the sample tank, and a stirring blade arranged on the output end of the motor; the stirring blade is fixedly connected to the motor;

[0011] The vacuum mechanism comprises: a plurality of sample temporary storage balls, a vacuum pump connected to the sample temporary storage balls, and a sample temporary storage tube arranged on the sample temporary storage balls; the plurality of sample temporary storage balls are arranged in a circular array, and connecting tubes are arranged between adjacent sample temporary storage balls, and the connecting tubes are respectively fixedly connected to adjacent sample temporary storage balls;

[0012] The detection mechanism includes: a sample storage pool, a plurality of sample detection blocks arranged on the sample storage pool, and an electrochemical sensor arranged on the sample detection block; a second delivery tube is arranged on the sample detection block, and both ends of the second delivery tube are fixedly connected to the sample detection block and the sample temporary storage ball respectively.

[0013] In a preferred embodiment of the utility model, a first delivery pipe is arranged at the bottom of the sample tank, a first on-off switch is arranged on the first delivery pipe, and the first delivery pipe is fixedly connected to the sample tank.

[0014] In a preferred embodiment of the utility model, the motor is provided with a shell, the shell is fixedly connected to the motor, the shell is fixedly connected to the sample storage pool, and the shell is fixedly connected to the sample tank; the shell is provided with a feed port, and the feed port is fixedly connected to the shell.

[0015] In a preferred embodiment of the utility model, a liquid separation cavity is provided at the bottom of the first delivery tube, a plurality of liquid separation funnels are provided on the liquid separation cavity, the first delivery tube is fixedly connected to the liquid separation cavity, and the liquid separation funnels are fixedly connected to the liquid separation cavity.

[0016] In a preferred embodiment of the utility model, the sample temporary storage tube is fixedly connected to the sample temporary storage ball; the sample temporary storage tube is fixedly connected to the bottom end of the separating funnel.

[0017] In a preferred embodiment of the utility model, a second on-off switch is provided on the connecting pipe, and the second on-off switch is connected to the connecting pipe.

[0018] In a preferred embodiment of the utility model, the connecting pipe is fixedly connected to the vacuum pump.

[0019] In a preferred embodiment of the utility model, a third on-off switch is provided on the second delivery pipe, and the third on-off switch is connected to the second delivery pipe.

[0020] In a preferred embodiment of the utility model, a sample detection slot is provided on the sample detection block, and the sample detection slot matches the position of the electrochemical sensor.

[0021] In a preferred embodiment of the present invention, a third delivery tube is provided on the sample detection block, and the third delivery tube is fixedly connected to the sample detection block and the sample storage pool respectively.

[0022] The utility model solves the defects existing in the background technology, and has the following beneficial effects:

[0023] (1) The utility model is provided with a vacuum mechanism, which creates a low-pressure environment through a vacuum pump, so that the sample solution can be more easily sucked into the sample temporary storage ball. Compared with the traditional device, the design of the vacuum mechanism of the utility model accelerates the sample transmission process and improves the detection efficiency.

[0024] (2) The utility model is provided with a connecting tube and a second on-off switch. The connecting tube uses the principle of communicating vessels to ensure that the sample solution in each sample temporary storage ball is highly consistent; the second on-off switch is used to control the on-off of the connecting tube to ensure that the samples in each temporary storage ball are independent of each other. Compared with the traditional device, the design of the connecting tube in the utility model realizes the simultaneous addition of the same amount of samples to ensure the detection accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The utility model is further described below in conjunction with the accompanying drawings and embodiments;

[0026] Figure 1 It is a half-section diagram of the overall three-dimensional structure of a preferred embodiment of the utility model;

[0027] Figure 2 It is a structural diagram of a stirring mechanism of a preferred embodiment of the utility model;

[0028] Figure 3 It is a structural diagram of the vacuum mechanism of the preferred embodiment of the utility model;

[0029] Figure 4 It is a structural diagram of a detection mechanism of a preferred embodiment of the utility model;

[0030] In the figure: 1. stirring mechanism; 2. vacuum mechanism; 3. detection mechanism; 4. sample tank; 5. motor; 6. third delivery pipe; 7. first delivery pipe; 8. first on-off switch; 9. housing; 10. liquid separation chamber; 11. separating funnel; 12. sample temporary storage ball; 13. vacuum pump; 14. sample temporary storage tube; 15. connecting tube; 16. second on-off switch; 17. sample storage pool; 18. sample detection block; 19. electrochemical sensor; 20. second delivery pipe; 21. third on-off switch. DETAILED DESCRIPTION

[0031] The present invention will now be further described in detail in conjunction with the accompanying drawings and embodiments. These drawings are simplified schematic diagrams that only illustrate the basic structure of the present invention in a schematic manner, and therefore only show the components related to the present invention.

[0032] like Figure 1 As shown, an exosome protein concentration detection device comprises: a stirring mechanism 1, a vacuum mechanism 2, and a detection mechanism 3;

[0033] like Figure 2 As shown, the stirring mechanism 1 includes a sample tank 4, a motor 5 disposed on the sample tank 4, and a stirring blade disposed on the output end of the motor 5; the stirring blade is fixedly connected to the motor 5. The stirring blade is driven to rotate by the motor 5 to achieve full stirring of the sample solution, ensure uniform distribution of the exosome protein in the sample, and provide accurate samples for subsequent detection.

[0034] A first delivery pipe 7 is arranged at the bottom of the sample tank 4, and a first on-off switch 8 is arranged on the first delivery pipe 7. The first delivery pipe 7 is fixedly connected to the sample tank 4; the first delivery pipe 7 is used to deliver the stirred sample to the subsequent processing component, and the first on-off switch 8 controls the on-off of the delivery pipe to prevent leakage of the sample during stirring.

[0035] The motor 5 is provided with a housing 9, which is fixedly connected to the motor 5, the housing 9 is fixedly connected to the sample storage pool 17, and the housing 9 is fixedly connected to the sample tank 4; the housing 9 is provided with a feed port, which is fixedly connected to the housing 9. The housing 9 protects the motor 5 and fixes the motor 5, the sample tank 4 and the sample storage pool 17, providing overall structural stability; the feed port facilitates the addition of samples, improving the convenience of the device.

[0036] A liquid separation chamber 10 is provided at the bottom of the first delivery tube 7, and a plurality of liquid separation funnels 11 are provided on the liquid separation chamber 10. The first delivery tube 7 is fixedly connected to the liquid separation chamber 10, and the liquid separation funnels 11 are fixedly connected to the liquid separation chamber 10. The sample is separated, and the funnel-shaped design is more convenient for liquid transmission.

[0037] like Figure 3As shown, the vacuum mechanism 2 includes: a plurality of sample temporary storage balls 12, a vacuum pump 13 connected to the sample temporary storage balls 12, and a sample temporary storage tube 14 arranged on the sample temporary storage balls 12; the plurality of sample temporary storage balls 12 are distributed along a circumferential array, and connecting tubes 15 are arranged between adjacent sample temporary storage balls 12, and the connecting tubes 15 are respectively fixedly connected to adjacent sample temporary storage balls 12. The vacuum pump 13 creates a low-pressure environment, so that the sample solution is more easily sucked into the sample temporary storage balls 12, which is convenient for subsequent diversion and detection.

[0038] The sample temporary storage tube 14 is fixedly connected to the sample temporary storage ball 12; the sample temporary storage tube 14 is fixedly connected to the bottom end of the separating funnel 11. The separating funnel 11 is connected to the sample temporary storage ball 12 to ensure that the sample can smoothly enter the temporary storage ball.

[0039] The connecting tube 15 is provided with a second on-off switch 16, which is connected to the connecting tube 15. The connecting tube 15 uses the communicating vessel principle to ensure that the sample solution in each sample temporary storage ball 12 is highly consistent; the second on-off switch 16 is used to control the on-off of the connecting tube 15 to ensure that the samples in each temporary storage ball are independent of each other.

[0040] The connecting pipe 15 is fixedly connected to the vacuum pump 13 .

[0041] like Figure 4 As shown, the detection mechanism 3 includes: a sample storage pool 17, a plurality of sample detection blocks 18 arranged on the sample storage pool 17, and an electrochemical sensor 19 arranged on the sample detection block 18; a second delivery tube 20 is arranged on the sample detection block 18, and the two ends of the second delivery tube 20 are respectively fixedly connected to the sample detection block 18 and the sample temporary storage ball 12. : The electrochemical sensor 19 on the sample detection block 18 can quickly and accurately detect the concentration of exosome protein in the sample. The setting of the second delivery tube 20 and the third on-off switch 21 ensures that the sample can be smoothly transferred from the sample temporary storage ball 12 to the sample detection block 18. The matching design of the sample detection tank and the electrochemical sensor 19 ensures the accuracy of the detection result. The third delivery tube 6 transports the detected sample to the sample storage pool 17, which is convenient for subsequent sample management and processing. It is used to store the detected sample for subsequent processing. The electrochemical sensor 19 can quickly and accurately detect the concentration of exosome protein in the sample; the sample detection block 18 provides support for the electrochemical sensor 19 and ensures that the sample is in effective contact with the sensor.

[0042] The second delivery pipe 20 is provided with a third on-off switch 21, which is connected to the second delivery pipe 20. By turning on all the third on-off switches 21 at the same time, the samples entering the sample detection slot for detection can enter at the same time and in the same amount, thereby achieving the effect of adding samples in the same amount at the same time to ensure the detection accuracy.

[0043] The sample detection block 18 is provided with a sample detection slot, which matches the position of the electrochemical sensor 19. The sample detection slot matches the electrochemical sensor 19 to ensure that the sensor can accurately detect the concentration of exosome proteins in the sample.

[0044] The sample detection block 18 is provided with a third delivery tube 6, which is fixedly connected to the sample detection block 18 and the sample storage pool 17. The third delivery tube 6 delivers the tested sample to the sample storage pool 17, which is convenient for subsequent sample management and processing.

[0045] When the utility model is used, the first on-off switch 8, the second on-off switch 16 and the third on-off switch 21 are all in the closed state; the feed port on the housing 9 is opened, the sample solution to be detected is added into the sample tank 4, and then the feed port is closed; the motor 5 is turned on to rotate the stirring blades in the sample tank 4 to fully stir the sample solution; after the stirring is completed, the vacuum pump 13 is turned on to create a low-pressure environment, and then the vacuum pump 13 is turned off; the first on-off switch 8 is turned on, and the sample solution enters the liquid separation chamber 10 through the first delivery pipe 7, and the liquid separation funnel in the liquid separation chamber 10 11 diverts the sample solution to each sample temporary storage ball 12; turns on the second on-off switch 16, the bottoms of each sample temporary storage ball 12 are interconnected, and through the principle of communicating vessels, the solution sample content in each sample temporary storage ball 12 is made the same, and the second on-off switch 16 is closed; turns on all the third on-off switches 21, and the sample enters the sample detection tank at the same time and in the same amount through the second delivery pipe 20, the electrochemical sensor 19 detects the concentration of exosome protein in the sample, and the third delivery pipe 6 delivers the detected sample to the sample storage pool 17, which is convenient for subsequent sample management and processing.

[0046] The above is based on the ideal embodiment of the utility model. Through the above description, relevant personnel can make various changes and modifications without deviating from the technical concept of the utility model. The technical scope of this utility model is not limited to the content in the specification, and the technical scope must be determined according to the scope of the claims.

Claims

1. A device for detecting exosome protein concentration, comprising: The stirring mechanism (1), the vacuum mechanism (2), and the detection mechanism (3) are characterized by: The stirring mechanism (1) comprises: a sample tank (4), a motor (5) arranged on the sample tank (4), and a stirring blade arranged on the output end of the motor (5); the stirring blade is fixedly connected to the motor (5); The vacuum mechanism (2) comprises: a plurality of sample temporary storage balls (12), a vacuum pump (13) connected to the sample temporary storage balls (12), and a sample temporary storage tube (14) arranged on the sample temporary storage balls (12); the plurality of sample temporary storage balls (12) are distributed in a circular array, connecting tubes (15) are arranged between adjacent sample temporary storage balls (12), and the connecting tubes (15) are respectively fixedly connected to adjacent sample temporary storage balls (12); The detection mechanism (3) comprises: a sample storage pool (17), a plurality of sample detection blocks (18) arranged on the sample storage pool (17), and an electrochemical sensor (19) arranged on the sample detection block (18); a second delivery tube (20) is arranged on the sample detection block (18), and two ends of the second delivery tube (20) are respectively fixedly connected to the sample detection block (18) and the sample temporary storage ball (12).

2. The exosome protein concentration detection device according to claim 1, characterized in that: A first delivery pipe (7) is arranged at the bottom of the sample tank (4), a first on-off switch (8) is arranged on the first delivery pipe (7), and the first delivery pipe (7) is fixedly connected to the sample tank (4).

3. The exosome protein concentration detection device according to claim 1, characterized in that: The motor (5) is provided with a housing (9), the housing (9) is fixedly connected to the motor (5), the housing (9) is fixedly connected to the sample storage pool (17), and the housing (9) is fixedly connected to the sample tank (4); the housing (9) is provided with a feed port, and the feed port is fixedly connected to the housing (9).

4. The exosome protein concentration detection device according to claim 2, characterized in that: A liquid separation chamber (10) is provided at the bottom of the first delivery tube (7), a plurality of liquid separation funnels (11) are provided on the liquid separation chamber (10), the first delivery tube (7) is fixedly connected to the liquid separation chamber (10), and the liquid separation funnels (11) are fixedly connected to the liquid separation chamber (10).

5. The exosome protein concentration detection device according to claim 4, characterized in that: The sample temporary storage tube (14) is fixedly connected to the sample temporary storage ball (12); the sample temporary storage tube (14) is fixedly connected to the bottom end of the separating funnel (11).

6. The exosome protein concentration detection device according to claim 1, characterized in that: The connecting pipe (15) is provided with a second on-off switch (16), and the second on-off switch (16) is connected to the connecting pipe (15).

7. The exosome protein concentration detection device according to claim 1, characterized in that: The connecting pipe (15) is fixedly connected to the vacuum pump (13).

8. The exosome protein concentration detection device according to claim 1, characterized in that: The second delivery pipe (20) is provided with a third on-off switch (21), and the third on-off switch (21) is connected to the second delivery pipe (20).

9. The exosome protein concentration detection device according to claim 1, characterized in that: The sample detection block (18) is provided with a sample detection slot, and the position of the sample detection slot matches that of the electrochemical sensor (19).

10. The exosome protein concentration detection device according to claim 1, characterized in that: The sample detection block (18) is provided with a third delivery tube (6), and the third delivery tube (6) is fixedly connected to the sample detection block (18) and the sample storage pool (17) respectively.

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