Cell exosome extraction device

Abstract

The utility model belongs to the field of cell separation and extraction, and particularly relates to a cell exosome extraction device which comprises a base, an installation cavity is formed in the middle of the base, a rotating seat is installed in the middle of the installation cavity, a driving assembly used for driving the rotating seat to rotate is installed in the rotating seat, and a containing groove is formed in the top end of the rotating seat. A test tube is mounted in the placement groove; the supporting assembly is arranged above the rotating seat and is used for supporting the top ends of the test tubes; the test tube is supported by arranging the supporting assembly, so that the test tube is more stable in the rotating process; in addition, after centrifugal work is completed, the supporting assembly can move downwards, then the top ends of the test tubes leak out, taking is convenient, the problem that the test tubes are inconvenient to take is solved, and the separation and extraction efficiency is improved to a certain degree.

Description

Technical Field

[0001] The utility model relates to the field of cell separation and extraction, in particular to an extracellular vesicle extraction device. Background Art

[0002] Cell exosome extraction technology, stem cell exosome extraction refers to the process of separating and extracting exosomes from stem cell culture fluid or stem cell culture supernatant. Exosomes are a type of small vesicles released by cells, containing lipid bilayer-encapsulated bioactive molecules such as miRNA, proteins and extracellular matrix components. Exosomes have a wide range of biological functions and have great application potential in the fields of regenerative medicine, drug delivery and disease treatment.

[0003] The extraction of exosomes requires the use of a separation and extraction device. Cell residues are removed by low-speed centrifugation and filtration to obtain a cleaner supernatant. The exosomes are then enriched and purified by ultracentrifugation, density gradient centrifugation or commercial purification tools (such as columns or magnetic beads). These methods are used to separate exosomes from other cell debris and impurities.

[0004] The current stem cell exosome separation and extraction device needs to fix the centrifuge tube body in the separation device, and use differential centrifugation and density centrifugation to allow different components in the sample to settle to their respective isopycnic areas. During the extraction of exosomes, the centrifuge tube needs to be fixed in the groove opened by the centrifuge rotor. However, after the separation device is finished working, the centrifuge tube is generally removed from the separation device. The upper end of the centrifuge tube is less exposed, and after the centrifugation is completed, it is difficult to hold the centrifuge tube, which is not convenient. The applicability is not high, which affects the processing efficiency to a certain extent. Therefore, a cell exosome extraction device is proposed to address the above problems. Utility Model Content

[0005] In order to make up for the shortcomings of the prior art, the utility model proposes a cell extracellular vesicle extraction device to solve the problems that the upper end of the centrifuge tube is less exposed and fits with the separation device, it is difficult to hold the centrifuge tube after centrifugation is completed, it is not suitable for use, and the processing efficiency is affected to a certain extent.

[0006] The technical solution adopted by the utility model to solve the technical problem is: the cell exosome extraction device described in the utility model comprises a base, a mounting cavity is provided in the middle of the base, a rotating seat is installed in the middle of the mounting cavity, a driving component for driving the rotating seat to rotate is installed inside the rotating seat, a placement groove is provided at the top of the rotating seat, and a test tube is installed in the placement groove;

[0007] The support assembly is arranged above the rotating seat and is used to support the top end of the test tube.

[0008] Preferably, the support assembly includes a support plate, a through hole is formed on the support plate, the through hole is adapted to the placement groove, a connecting ring is fixedly connected to the outer wall of the support plate, a support guide rail is engaged with the outer side of the connecting ring, fixed blocks are symmetrically fixedly connected to both sides of the support guide rail, a hydraulic rod is installed under the fixed block, the bottom end of the hydraulic rod is fixedly connected to the bottom of the installation cavity, and the top telescopic end of the hydraulic rod is fixedly connected to the fixed block.

[0009] Preferably, the top of the rotating seat is symmetrically provided with guide grooves, the bottom of the supporting plate is symmetrically fixedly connected with guide rods, and the guide rods are slidably arranged in the guide grooves.

[0010] Preferably, a mounting groove is provided on the side wall of the connecting ring, a ball is movably installed in the mounting groove, and the ball is in contact with the inner wall of the supporting guide rail.

[0011] Preferably, a cover plate is rotatably mounted on the bottom end of the base.

[0012] Preferably, a transparent observation window is fixedly installed in the middle of the cover plate.

[0013] Preferably, a limit assembly is provided at the bottom of the placement slot, and the limit assembly includes movable cavities symmetrically opened on both sides of the bottom of the placement slot, a limit block is slidably installed in the movable cavity, a spring for resetting the limit block is fixedly installed inside the movable cavity, and the top of the limit block is arranged to be inclined.

[0014] The utility model is beneficial in that:

[0015] 1. The utility model supports the test tube by arranging a support assembly, and supports the top of the test tube by the support plate when in use, so that the test tube is more stable during rotation; in addition, after the centrifugation work is completed, the fixed block is driven downward by the hydraulic rod, and then the support guide rail, the connecting ring and the support plate are driven downward together, and the support plate moves to the lower part of the bottom end of the test tube, so that the top of the test tube leaks out, which is convenient for taking, solves the problem of inconvenient test tube taking, and improves the separation and extraction efficiency to a certain extent.

[0016] 2. The utility model clamps and fixes the test tube by setting a limit assembly. When the test tube is inserted into the placement slot, the bottom of the test tube contacts the inclined surface of the top of the limit block, pushing the limit block to move toward the inside of the movable cavity. The spring is compressed, and then the limit block is pushed to clamp on both sides of the test tube through the spring rebound action, thereby limiting the test tube and allowing the test tube to be stably installed in the placement slot. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0018] Figure 1 It is a schematic diagram of the overall structure of the first embodiment;

[0019] Figure 2 It is a partial cross-sectional view of the first embodiment;

[0020] Figure 3 For Example 1 Figure 2 A schematic diagram of the structure enlargement in the middle;

[0021] Figure 4 It is a partial cross-sectional view of the rotating seat of the first embodiment;

[0022] Figure 5 For Example 1 Figure 4 Enlarged schematic diagram of the structure at point B in the middle.

[0023] In the figure: 1. base; 2. cover plate; 3. observation window; 4. rotating seat; 5. placement slot; 6. test tube; 7. support assembly; 71. support plate; 72. connecting ring; 73. support guide rail; 74. hydraulic rod; 75. fixing block; 76. mounting slot; 77. ball bearing; 78. through hole; 79. guide rod; 710. guide slot; 8. limit assembly; 81. movable cavity; 82. limit block; 83. spring; 9. mounting cavity. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0025] Embodiment 1

[0026] See also Figure 1-4 As shown, an exosome extraction device comprises a base 1, a mounting cavity 9 is provided in the middle of the base 1, a rotating seat 4 is installed in the middle of the mounting cavity 9, a driving component for driving the rotating seat 4 to rotate is installed inside the rotating seat 4, a placement groove 5 is provided at the top of the rotating seat 4, and a test tube 6 is installed in the placement groove 5;

[0027] A support assembly 7 is disposed above the rotating seat 4 and is used to support the top end of the test tube 6;

[0028] During operation, the stem cell culture fluid is injected into the test tube 6, and then the test tube 6 is vertically placed in the placement groove 5 provided on the rotating seat 4 from above the support component 7, and then the rotating seat 4 and the test tube 6 are driven to rotate together by the driving component, and the support component 7 supports the top of the test tube 6, so that the test tube 6 is more stable during the rotation process, and the stem cell culture fluid in the test tube 6 is centrifuged to remove cell residues to obtain a cleaner supernatant, which is convenient for the subsequent separation and extraction of exosomes from the supernatant.

[0029] The support assembly 7 includes a support plate 71, on which a through hole 78 is provided, and the through hole 78 is adapted to the placement groove 5. The outer wall of the support plate 71 is fixedly connected to a connecting ring 72, and the outer side of the connecting ring 72 is engaged with a supporting guide rail 73. The two sides of the supporting guide rail 73 are symmetrically fixedly connected to fixed blocks 75. A hydraulic rod 74 is installed below the fixed block 75, and the bottom end of the hydraulic rod 74 is fixedly connected to the bottom of the installation cavity 9, and the top telescopic end of the hydraulic rod 74 is fixedly connected to the fixed block 75. When working, the test tube 6 is vertically placed in the corresponding placement groove 5 below through the through hole 78. When the test tube 6 is placed in the corresponding placement groove 5 below, When the bottom of the tube 6 contacts the bottom of the placement groove 5, the support plate 71 is located at the top of the test tube 6, which is convenient for limiting the support of the top of the test tube 6, and the connecting ring 72 on the side of the support plate 71 rotates inside the support guide rail 73, which will not affect the centrifugal rotation process; in addition, after the centrifugal work is completed, the hydraulic rod 74 drives the fixed block 75 to move downward, and then drives the support guide rail 73, the connecting ring 72 and the support plate 71 to move downward together, and the support plate 71 moves to the lower part of the bottom end of the test tube 6, so that the top of the test tube 6 leaks out, which is convenient for taking, solves the problem of inconvenient test tube 6 taking, and improves the separation and extraction efficiency to a certain extent.

[0030] The top of the rotating seat 4 is symmetrically provided with a guide groove 710, and the bottom end of the support plate 71 is symmetrically fixedly connected with a guide rod 79, and the guide rod 79 is slidably set in the guide groove 710; when working, the guide rod 79 and the guide groove 710 cooperate to facilitate limiting the movement direction of the support plate 71, so that the support plate 71 can move up and down and can rotate together with the rotating seat 4.

[0031] The side wall of the connecting ring 72 is provided with a mounting groove 76, in which a ball 77 is movably installed, and the ball 77 is in contact with the inner wall of the supporting guide rail 73; during operation, the friction between the connecting ring 72 and the inner wall of the supporting guide rail 73 is reduced by setting the ball 77, so as to facilitate rotation.

[0032] A cover plate 2 is rotatably mounted on the bottom end of the base 1 ; when in operation, the installation cavity 9 is completely closed by the cover plate 2 .

[0033] A transparent observation window 3 is fixedly installed in the middle of the cover plate 2; during operation, the internal conditions of the installation cavity 9 can be easily viewed through the observation window 3.

[0034] Embodiment 2

[0035] See also Figure 5 As shown, compared with Example 1, as another implementation of the utility model, a limiting component 8 is provided at the bottom of the placement slot 5, and the limiting component 8 includes an active cavity 81 symmetrically opened on both sides of the bottom of the placement slot 5, and a limiting block 82 is slidably installed in the active cavity 81, and a spring 83 is fixedly installed inside the active cavity 81 to reset the limiting block 82, and the top of the limiting block 82 is arranged to be inclined; during operation, when the test tube 6 is inserted into the placement slot 5, the bottom of the test tube 6 contacts the inclined surface of the top of the limiting block 82, pushing the limiting block 82 to move toward the inside of the active cavity 81, and the spring 83 is compressed, and then the limiting block 82 is pushed to clamp on both sides of the test tube 6 through the rebound action of the spring 83, so as to limit the test tube 6 and make the test tube 6 stably installed in the placement slot 5.

[0036] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0037] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments, and the above embodiments and descriptions are only for explaining the principle of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, and these changes and improvements fall within the scope of the utility model to be protected.

Claims

1. A device for extracting extracellular vesicles, characterized in that: The invention comprises a base (1), a mounting cavity (9) is provided in the middle of the base (1), a rotating seat (4) is installed in the middle of the mounting cavity (9), a driving assembly for driving the rotating seat (4) to rotate is installed inside the rotating seat (4), a placement groove (5) is provided at the top of the rotating seat (4), and a test tube (6) is installed in the placement groove (5); A support assembly (7) is arranged above the rotating seat (4) and is used to support the top end of the test tube (6); The support assembly (7) comprises a support plate (71), a through hole (78) is provided on the support plate (71), the through hole (78) is adapted to the placement groove (5), a connecting ring (72) is fixedly connected to the outer wall of the support plate (71), a supporting guide rail (73) is engaged on the outer side of the connecting ring (72), fixed blocks (75) are symmetrically fixedly connected to the two sides of the supporting guide rail (73), a hydraulic rod (74) is installed below the fixed block (75), the bottom end of the hydraulic rod (74) is fixedly connected to the bottom of the installation cavity (9), and the top telescopic end of the hydraulic rod (74) is fixedly connected to the fixed block (75).

2. The device for extracting extracellular vesicles according to claim 1, characterized in that: The top of the rotating seat (4) is symmetrically provided with a guide groove (710), and the bottom of the supporting plate (71) is symmetrically fixedly connected with a guide rod (79), and the guide rod (79) is slidably arranged in the guide groove (710).

3. The device for extracting extracellular vesicles according to claim 2, characterized in that: A mounting groove (76) is provided on the side wall of the connecting ring (72), a ball (77) is movably mounted in the mounting groove (76), and the ball (77) is in contact with the inner wall of the supporting guide rail (73).

4. The device for extracting extracellular vesicles according to claim 3, characterized in that: A cover plate (2) is rotatably mounted on the bottom end of the base (1).

5. The device for extracting extracellular vesicles according to claim 4, characterized in that: A transparent observation window (3) is fixedly installed in the middle of the cover plate (2).

6. The device for extracting extracellular vesicles according to claim 5, characterized in that: A limit assembly (8) is provided at the bottom of the placement groove (5), and the limit assembly (8) comprises movable cavities (81) symmetrically arranged on both sides of the bottom of the placement groove (5), a limit block (82) is slidably installed in the movable cavity (81), a spring (83) for returning the limit block (82) is fixedly installed inside the movable cavity (81), and the top end of the limit block (82) is arranged to be inclined.

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