Stem cell rolling culture tank

Abstract

The utility model belongs to the technical field of stem cell culture, and particularly relates to a stem cell rolling culture tank which comprises a fixed block, a stem cell culture tank is arranged in the middle of the fixed block; a sealing cover is arranged at the top of the stem cell culture tank; a first servo motor is fixedly connected to one side of the top of the fixed block; the output end of the first servo motor is fixedly connected with a belt shaft; the top of the fixed block is rotationally connected with a rotating shaft; the interior of the rotating shaft is in threaded connection with a lead screw; the middle part of the belt shaft is rotationally connected with a rotating belt; when stem cells are cultured, slight stirring needs to be carried out, and the interior of the tank body is not easy to clean when an experiment is finished, so that the interior of the stem cell culture tank and the stirring block are cleaned through rotation of the cleaning claw in the stem cell culture tank when the experiment is finished, and the interior of the stem cell culture tank is kept clean; the efficiency of next work is improved, and the possibility of inaccurate experimental data caused by residual stains on the inner side of the stem cell culture tank is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell culture, in particular to a stem cell rolling culture tank. Background Art

[0002] Stem cells are a type of self-renewing cell that is infinite or immortal. They mainly come from embryos, fetuses or adults. They have some extremely important functions for the human body, such as promoting hematopoiesis, regulating immunity, regulating metabolism, etc. They can also be used to prevent diseases such as hypertension.

[0003] Currently, stem cell rolling culture tanks are mainly used to improve culture efficiency by rolling and slightly shaking the tank body so that the stem cells inside can fully contact with the nutrient solution therein.

[0004] However, the process of stem cell culture is complicated and requires strict requirements. In particular, if there are some stains inside the tank, it will not only affect the culture efficiency, but in serious cases it may even contaminate the stem cells and cause the samples to be scrapped. Therefore, a stem cell rolling culture tank is proposed to address the above problems. Utility Model Content

[0005] In order to make up for the deficiencies of the prior art and solve at least one technical problem raised in the background technology, the utility model provides a stem cell rolling culture tank.

[0006] The technical solution adopted by the utility model to solve its technical problems is: a stem cell rolling culture tank described in the utility model comprises a fixed block; a stem cell culture tank is provided in the middle of the fixed block; a sealing cover is provided on the top of the stem cell culture tank; a first servo motor is fixedly connected to one side of the top of the fixed block; a belt shaft is fixedly connected to the output end of the first servo motor; a rotating shaft is rotatably connected to the top of the fixed block; a lead screw is connected to the internal thread of the rotating shaft; a rotating belt is rotatably connected to the middle of the belt shaft; a rotating belt is rotatably connected to the middle of the rotating shaft; a limiting ring is fixedly connected to the middle of the lead screw; a fixed block is fixedly connected to the middle of the lead screw; an end of the fixed block away from the lead screw is fixedly connected to a rotating shaft; a cleaning claw is rotatably connected to the middle of the rotating shaft; a spring is fixedly connected to the middle of the cleaning claw; an end of the lead screw away from the rotating shaft is fixedly connected to a spring; a stirring block is fixedly connected to the inside of the stem cell culture tank.

[0007] Preferably, a second servo motor is fixedly connected to the bottom of the screw; a guide plate is fixedly connected to the middle part of the inner side of the fixed block; a cleaning claw is slidably connected to the inside of the guide plate; a driving shaft is fixedly connected to the output end of the second servo motor; a driving block is fixedly connected to the bottom of the driving shaft; a rotating pin is rotatably connected to the middle part of the driving block; a connecting rod is fixedly connected to the top of the rotating pin; and a cleaning claw is fixedly connected to the end of the connecting rod away from the rotating pin.

[0008] Preferably, a rotatable telescopic rod is fixedly connected to the bottom inner side of the fixed block; a threaded column is fixedly connected to the top of the rotatable telescopic rod; a threaded cap is provided at the bottom of the stem cell culture tank; a threaded column is threadedly connected inside the threaded cap; a clamping block is fixedly connected to one side of the top of the rotatable telescopic rod; a clamping slot is provided at the bottom inner side of the fixed block; and a clamping block is slidably connected inside the clamping slot.

[0009] Preferably, a dryer shell is fixedly connected to the middle portion of the guide plate; a fixing column is fixedly connected to the inner top of the dryer shell; a fan motor is fixedly connected to the end of the fixing column away from the dryer shell; a driving column is fixedly connected to the output end of the fan motor; a fan blade is fixedly connected to the end of the driving column away from the fan motor; and a rotating shaft is fixedly connected to the bottom of the dryer shell.

[0010] Preferably, a special-shaped sealing gasket is fixedly connected to the bottom of the sealing cover; a stem cell culture tank is fixedly connected to the bottom of the sealing cover; a thread groove is fixedly connected to the top of the stem cell culture tank; and a thread ring is threadedly connected to the outer side of the thread groove.

[0011] Preferably, an ultraviolet lamp is fixedly connected to the bottom of the driving block.

[0012] Preferably, a rubber pad is fixed to the inner bottom of the cleaning claw.

[0013] The utility model is beneficial in that:

[0014] 1. The stem cell rolling culture tank described in the utility model needs to be slightly stirred when culturing stem cells, and at the end of the experiment, the inside of the tank is not easy to clean. Therefore, by using this device at the end of the experiment, the cleaning claws rotate inside the stem cell culture tank to clean the inside of the stem cell culture tank and the stirring block, thereby keeping the inside of the stem cell culture tank clean, improving the efficiency of the next work, and reducing the possibility of inaccurate experimental data due to stains remaining on the inside of the stem cell culture tank.

[0015] 2. The stem cell rolling culture tank described in the utility model can be directly adjusted through this device when adjusting the approach or distance of multiple groups of cleaning claws, and rely on the force provided by the second servo motor to make the clamping of the stem cell culture tank more firm and the cleaning of the inside of the stem cell culture tank more thorough. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0017] Figure 1 This is a schematic diagram of the three-dimensional structure of the stem cell culture tank in the utility model;

[0018] Figure 2 It is a three-dimensional cross-sectional structural schematic diagram of the dryer shell in the utility model;

[0019] Figure 3 This is a schematic diagram of the structure of the driving block in the utility model;

[0020] Figure 4 This is a schematic diagram of the structure of the rotatable telescopic rod in the utility model;

[0021] Figure 5 for Figure 2 Enlarged view of point A.

[0022] In the figure: 1. stem cell culture tank; 11. sealing cover; 12. first servo motor; 13. belt shaft; 14. rotating belt; 15. rotating shaft; 16. lead screw; 17. limiting ring; 18. fixed block; 19. rotating shaft; 101. cleaning claw; 102. spring; 103. stirring block; 2. guide plate; 21. second servo motor; 22. driving shaft; 23. driving block; 24. rotating pin; 25. connecting rod; 3. threaded cap; 31. threaded column; 32. rotatable telescopic rod; 33. clamping block; 34. clamping groove; 4. dryer housing; 41. fixing column; 42. fan motor; 43. driving column; 44. fan blade; 45. filter plate; 5. special-shaped sealing gasket; 51. threaded ring; 52. threaded groove; 6. ultraviolet lamp; 7. rubber pad. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] like Figure 1-5As shown, a stem cell rolling culture tank comprises a fixed block 18; a stem cell culture tank 1 is provided in the middle of the fixed block 18; a sealing cover 11 is provided on the top of the stem cell culture tank 1; a first servo motor 12 is fixedly connected to one side of the top of the fixed block 18; a belt shaft 13 is fixedly connected to the output end of the first servo motor 12; a rotating shaft 15 is rotatably connected to the top of the fixed block 18; a lead screw 16 is internally threadedly connected to the rotating shaft 15; a rotating belt 14 is rotatably connected to the middle of the belt shaft 13; a rotating belt 14 is rotatably connected to the middle of the rotating shaft 15 14; the middle of the lead screw 16 is fixedly connected to a limit ring 17; the middle of the lead screw 16 is fixedly connected to a fixed block 18; the fixed block 18 is fixedly connected to a rotating shaft 19 at one end away from the lead screw 16; the middle of the rotating shaft 19 is rotatably connected to a cleaning claw 101; the middle of the cleaning claw 101 is fixedly connected to a spring 102; the end of the lead screw 16 away from the rotating shaft 15 is fixedly connected to a spring 102; the inner side of the stem cell culture tank 1 is fixedly connected to a stirring block 103; when working, the first servo motor 12 drives the belt shaft 13 to start rotating, and the belt shaft 13 rotates to drive the rotating belt 14, the rotating belt The shaft 15 starts to rotate, so that the lead screw 16 moves up and down inside the rotating shaft 15, and the limiting ring 17 limits the lead screw 16. At this time, the fixed block 18 and the rotating shaft 19 rotate to make the multiple sets of cleaning claws 101 approach and extend into the inner side of the stem cell culture tank 1. At the same time, the multiple sets of cleaning claws 101 rotate to clean the inner side of the stem cell culture tank 1. During operation, the fixed block 18 and the rotating shaft 19 rotate to make the multiple sets of cleaning claws 101 move away and then descend to clamp the stem cell culture tank 1. At this time, the stem cell culture tank 1 reaches To achieve the purpose of rotation and rolling, the internal stirring block 103 will slowly stir the internal stem cell culture solution. Slight stirring is required when culturing stem cells, and at the end of the experiment, the inside of the tank is not easy to clean. Therefore, through this device, at the end of the experiment, the cleaning claw 101 rotates inside the stem cell culture tank 1 to clean the inside of the stem cell culture tank 1 and the stirring block 103, keeping the inside of the stem cell culture tank 1 clean, improving the efficiency of the next work, and reducing the possibility of inaccurate experimental data due to stains remaining on the inside of the stem cell culture tank 1.

[0025] like Figure 1-5As shown, a second servo motor 21 is fixedly connected to the bottom of the lead screw 16; a guide plate 2 is fixedly connected to the middle of the inner side of the fixed block 18; a cleaning claw 101 is slidably connected inside the guide plate 2; a driving shaft 22 is fixedly connected to the output end of the second servo motor 21; a driving block 23 is fixedly connected to the bottom of the driving shaft 22; a rotating pin 24 is rotatably connected to the middle of the driving block 23; a connecting rod 25 is fixedly connected to the top of the rotating pin 24; the cleaning claw 101 is fixedly connected to the end of the connecting rod 25 away from the rotating pin 24; when working, the second servo motor 21 drives the driving shaft 22 to rotate. The driving shaft 22 drives the driving block 23 to rotate. Due to the connection relationship between the driving block 23, the rotating pin 24, and the connecting rod 25, the connecting rod 25 will start to rotate with the driving block 23. At this time, the connecting rod 25 pulls the cleaning claws 101 to slide inside the guide plate 2, so that multiple groups of cleaning claws 101 are approached. The second servo motor 21 rotates at a small angle. When adjusting the approach or distance of the multiple groups of cleaning claws 101, this device can be used to directly adjust it, and rely on the force provided by the second servo motor 21 to make the clamping of the stem cell culture tank 1 more firm and the cleaning of the inside of the stem cell culture tank 1 cleaner.

[0026] like Figure 1-2 , Figure 4 As shown, a rotatable telescopic rod 32 is fixedly connected to the bottom of the inner side of the fixing block 18; a threaded column 31 is fixedly connected to the top of the rotatable telescopic rod 32; a threaded cap 3 is provided at the bottom of the stem cell culture tank 1; the threaded column 31 is threadedly connected inside the threaded cap 3; a clamping block 33 is fixedly connected to one side of the top of the rotatable telescopic rod 32; a clamping groove 34 is provided at the bottom of the inner side of the fixing block 18; the clamping block 33 is slidably connected inside the clamping groove 34; when working, the threaded cap 3 is first connected to the threaded column 31 by rotation, and then when the inside of the stem cell culture tank 1 needs to be cleaned, the rotatable The movable telescopic rod 32 is compressed to insert the clamping block 33 into the clamping slot 34, and the stem cell culture tank 1 is fixed inside. When working, the rotatable telescopic rod 32 is stretched to realize the rotation of the stem cell culture tank 1. When the inside of the stem cell culture tank 1 is cleaned, the stem cell culture tank 1 may follow the rotation of the cleaning claw 101, so the stem cell culture tank 1 is fixed and limited by the clamping block 33 and the clamping slot 34 to reduce the possibility of following the rotation. When the culture tank needs to be rotated and rolled, the clamping block 33 can also leave the clamping slot 34 to realize the rotation and rolling of the stem cell culture tank 1.

[0027] like Figure 2-3 , Figure 5As shown, a dryer casing 4 is fixedly connected to the middle part of the guide plate 2; a fixing column 41 is fixedly connected to the inner top of the dryer casing 4; a fan motor 42 is fixedly connected to the end of the fixing column 41 away from the dryer casing 4; a driving column 43 is fixedly connected to the output end of the fan motor 42; a fan blade 44 is fixedly connected to the end of the driving column 43 away from the fan motor 42; a filter plate 45 is fixedly connected to the bottom of the dryer casing 4; when working, the fan motor 42 fixed at the end of the fixing column 41 drives the driving column 43 and the fan blade 44 to start rotating, generating an airflow to be sprayed out through the filter plate 45. When the interior of the tank is cleaned, some water stains may remain. If it is not cleaned for a long time, it may also contaminate the culture tank. Therefore, the airflow is used to dry the interior of the culture tank to keep the tank clean.

[0028] like Figure 1-2 , Figure 4 As shown, a special-shaped sealing gasket 5 is fixedly connected to the bottom of the sealing cover 11; a stem cell culture tank 1 is fixedly connected to the bottom of the sealing cover 11; a threaded groove 52 is fixedly connected to the top of the stem cell culture tank 1; a threaded ring 51 is threadedly connected to the outer side of the threaded groove 52; when working, the sealing cover 11 is rotated to connect the threaded ring 51 and the threaded groove 52, and the special-shaped sealing gasket 5 is squeezed at the same time. Good sealing performance is required during the stem cell culture process, so the sealing performance is increased by squeezing the special-shaped sealing gasket 5. At the same time, due to the characteristics of the special-shaped sealing gasket 5, the rubber can seal most of the gaps, and the sealing performance of the tank body is more excellent.

[0029] like Figure 2 , Figure 5 As shown, an ultraviolet lamp 6 is fixedly connected to the bottom of the driving block 23; when working, the ultraviolet lamp 6 emits ultraviolet rays and rotates to irradiate the inside of the stem cell culture tank 1. During stem cell culture, not only stains will affect the culture efficiency, but some bacteria and fungi will also affect the experimental efficiency. Therefore, ultraviolet sterilization can greatly reduce the impact of bacteria on the experimental efficiency.

[0030] like Figure 2-5 As shown, a rubber pad 7 is fixedly connected to the inner bottom of the cleaning claw 101; when the cleaning claw 101 is working, it will play a buffering role when clamping the stem cell culture tank 1. When the cleaning claw 101 clamps the stem cell culture tank 1, the rubber pad 7 can play a role of buffering and increasing friction, which not only protects the outside of the tank body, but also makes the clamping more secure.

[0031] Working principle: the belt shaft 13 is driven by the first servo motor 12 to start rotating, and the rotation of the belt shaft 13 drives the rotating belt 14 and the rotating shaft 15 to start rotating, so that the lead screw 16 moves up and down inside the rotating shaft 15, and the limit ring 17 limits the lead screw 16. At this time, the multiple groups of cleaning claws 101 are brought close to each other through the rotation of the fixed block 18 and the rotating shaft 19, and are extended into the inner side of the stem cell culture tank 1. At the same time, the multiple groups of cleaning claws 101 rotate to clean the inner side of the stem cell culture tank 1. During operation, the multiple groups of cleaning claws 101 can be moved away by the rotation of the fixed block 18 and the rotating shaft 19, and then they are lowered to clamp the stem cell culture tank 1. At this time, the stem cell culture tank 1 achieves the purpose of rotation and rolling, and the internal stirring block 103 will slowly stir the internal stem cell culture solution. Slight stirring is required when culturing stem cells, and at the end of the experiment, the inside of the tank is not easy to clean, so this device is used at the end of the experiment. When the cleaning claw 101 rotates inside the stem cell culture tank 1, the inside of the stem cell culture tank 1 and the stirring block 103 are cleaned, the inside of the stem cell culture tank 1 is kept clean, the efficiency of the next work is improved, and the possibility of inaccurate experimental data caused by the stains remaining on the inside of the stem cell culture tank 1 is reduced. The second servo motor 21 drives the driving shaft 22 to rotate, and the driving shaft 22 drives the driving block 23 to rotate. Due to the connection relationship between the driving block 23, the rotating pin 24, and the connecting rod 25, the connecting rod 25 will start to rotate with the driving block 23. At this time, the connecting rod 25 pulls the cleaning claw 101 to slide inside the guide plate 2, so that multiple groups of cleaning claws 101 are approached. The second servo motor 21 has a small rotation angle. When adjusting the approach or distance of multiple groups of cleaning claws 101, this device can be directly adjusted, and the force provided by the second servo motor 21 is relied on to make the clamping of the stem cell culture tank 1 more firm and stable. The inner side of the stem cell culture tank 1 is cleaned more cleanly. The threaded cap 3 is connected to the threaded column 31 by rotation. At this time, when the inner side of the stem cell culture tank 1 needs to be cleaned, the rotatable telescopic rod 32 is compressed to insert the clamping block 33 into the clamping groove 34. The stem cell culture tank 1 is fixed inside. When working, the rotatable telescopic rod 32 is stretched to realize the rotation of the stem cell culture tank 1. When the inner side of the stem cell culture tank 1 is cleaned, the stem cell culture tank 1 may rotate with the cleaning claw 101. Therefore, the clamping block 33 and the clamping groove 34 are used to fix the stem cell culture tank 1. 4 is used to fix and limit the stem cell culture tank 1 to reduce the possibility of following the rotation. When the culture tank needs to be rotated and rolled, the card block 33 can also be used to leave the card slot 34 to realize the rotation and rolling of the stem cell culture tank 1. The fan motor 42 fixed at the end of the fixed column 41 drives the driving column 43 and the fan blades 44 to start rotating, and the airflow is generated to be ejected through the filter plate 45. When the interior of the tank is cleaned, some water stains may remain. If it is not cleaned for a long time, it may also pollute the culture tank. Therefore, the airflow is used to dry the interior of the culture tank to keep the tank clean. The sealing cover 11 is rotated.The thread ring 51 and the thread groove 52 are connected, and the special-shaped sealing gasket 5 is squeezed at the same time. Good sealing is required in the process of stem cell culture, so the sealing is increased by squeezing the special-shaped sealing gasket 5. At the same time, due to the characteristics of the special-shaped sealing gasket 5, the rubber can seal most of the gaps, and the sealing of the tank body is more excellent. The ultraviolet lamp 6 emits ultraviolet rays and rotates to irradiate the inside of the stem cell culture tank 1. During stem cell culture, not only stains will affect the culture efficiency, but some bacteria and fungi will also affect the experimental efficiency. Therefore, ultraviolet sterilization greatly reduces the impact of bacteria on the experimental efficiency. When the cleaning claw 101 clamps the stem cell culture tank 1, it will play a buffering role. When the cleaning claw 101 clamps the stem cell culture tank 1, the rubber pad 7 can play a role of buffering and increasing friction, which will not only protect the outside of the tank body, but also make the clamping more firm.

[0032] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments, and the above embodiments and descriptions are only for explaining the principle of the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, and these changes and improvements fall within the scope of the utility model to be protected.

Claims

1. A stem cell rolling culture tank, comprising a fixing block (18); characterized in that: The fixed block (18) has a stem cell culture tank (1) in the middle; the top of the stem cell culture tank (1) has a sealing cover (11); a first servo motor (12) is fixedly connected to one side of the top of the fixed block (18); a belt shaft (13) is fixedly connected to the output end of the first servo motor (12); a rotating shaft (15) is rotatably connected to the top of the fixed block (18); a lead screw (16) is internally threadedly connected to the rotating shaft (15); a rotating belt (14) is rotatably connected to the middle of the belt shaft (13); and a rotating shaft (15) is rotatably connected to the middle of the rotating shaft (15). A rotating belt (14) is connected; a limiting ring (17) is fixedly connected to the middle of the lead screw (16); a fixing block (18) is fixedly connected to the middle of the lead screw (16); an end of the fixing block (18) away from the lead screw (16) is fixedly connected to a rotating shaft (19); a cleaning claw (101) is rotatably connected to the middle of the rotating shaft (19); a spring (102) is fixedly connected to the middle of the cleaning claw (101); an end of the lead screw (16) away from the rotating shaft (15) is fixedly connected to the spring (102); and a stirring block (103) is fixedly connected to the inner side of the stem cell culture tank (1).

2. A stem cell rolling culture tank according to claim 1, characterized in that: A second servo motor (21) is fixedly connected to the bottom of the lead screw (16); a guide plate (2) is fixedly connected to the middle of the inner side of the fixed block (18); a cleaning claw (101) is slidably connected inside the guide plate (2); a driving shaft (22) is fixedly connected to the output end of the second servo motor (21); a driving block (23) is fixedly connected to the bottom of the driving shaft (22); a rotating pin (24) is rotatably connected to the middle of the driving block (23); a connecting rod (25) is fixedly connected to the top of the rotating pin (24); and a cleaning claw (101) is fixedly connected to one end of the connecting rod (25) away from the rotating pin (24).

3. The stem cell rolling culture tank according to claim 2, characterized in that: A rotatable telescopic rod (32) is fixedly connected to the bottom of the inner side of the fixed block (18); a threaded column (31) is fixedly connected to the top of the rotatable telescopic rod (32); a threaded cap (3) is provided at the bottom of the stem cell culture tank (1); the threaded column (31) is threadedly connected inside the threaded cap (3); a clamping block (33) is fixedly connected to one side of the top of the rotatable telescopic rod (32); a clamping slot (34) is provided at the bottom of the inner side of the fixed block (18); and the clamping block (33) is slidably connected inside the clamping slot (34).

4. The stem cell rolling culture tank according to claim 3, characterized in that: A dryer housing (4) is fixedly connected to the middle of the guide plate (2); a fixing column (41) is fixedly connected to the top of the inner side of the dryer housing (4); a fan motor (42) is fixedly connected to one end of the fixing column (41) away from the dryer housing (4); a driving column (43) is fixedly connected to the output end of the fan motor (42); a fan blade (44) is fixedly connected to one end of the driving column (43) away from the fan motor (42); and a filter plate (45) is fixedly connected to the bottom of the dryer housing (4).

5. The stem cell rolling culture tank according to claim 4, characterized in that: The bottom of the sealing cover (11) is fixedly connected to a special-shaped sealing gasket (5); the bottom of the sealing cover (11) is fixedly connected to a stem cell culture tank (1); the top of the stem cell culture tank (1) is fixedly connected to a thread groove (52); and the outer side of the thread groove (52) is threadedly connected to a thread ring (51).

6. The stem cell rolling culture tank according to claim 5, characterized in that: An ultraviolet lamp (6) is fixedly connected to the bottom of the driving block (23).

7. The stem cell rolling culture tank according to claim 6, characterized in that: A rubber pad (7) is fixedly connected to the inner bottom of the cleaning claw (101).

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