Hydrogel microsphere preparation device

Abstract

The utility model provides a hydrogel microsphere preparation device, relates to the field of hydrogel microsphere preparation, and solves the problems that hydrogel microspheres with highly uniform particle sizes cannot be prepared in the existing hydrogel microsphere preparation process, so that the lubrication performance of the hydrogel microspheres is poor, and the like. The hydrogel microsphere preparation device comprises: a reaction chamber; the microporous pipeline is arranged at the bottom of the reaction chamber and is communicated with the reaction chamber; the continuous phase conveying assembly is communicated with the reaction chamber; the dispersed phase conveying assembly is communicated with the reaction chamber through a microporous pipeline; in the using state, the continuous phase conveying assembly conveys a continuous phase into the reaction chamber, the dispersed phase conveying assembly evenly divides the dispersed phase through the micropore pipeline, and the divided dispersed phase is conveyed into the reaction chamber to react with the continuous phase. According to the scheme provided by the utility model, the hydrogel microspheres can be rapidly prepared, and meanwhile, the high uniformity of the particle size of the hydrogel microspheres is ensured, so that the lubricity of the hydrogel microspheres is improved.

Description

Technical Field

[0001] The utility model relates to the field of hydrogel microsphere preparation, in particular to a hydrogel microsphere preparation device. Background Art

[0002] Hydrogel microspheres are a type of hydrogel or composite hydrogel in the shape of a sphere or other geometric shape, and their diameter is generally in the micrometer or even nanometer range. Compared with macroscopic hydrogels, hydrogel microspheres have many advantages, such as better dispersibility and specific surface area in solution, which allows for faster response. The microspheres have a smooth surface and good drug carrier properties, and can be prepared into injectable biolubricants, drug controlled release carriers, cell culture scaffolds, and tissue engineering materials. However, the current methods for preparing hydrogel microspheres have the problem of being unable to generate hydrogel microspheres with highly uniform particle sizes during the preparation process, and the microspheres of different sizes formed are also prone to aggregation during the separation and stirring process, resulting in the inability to disperse in the subsequent process, which greatly affects the particle size of the hydrogel microspheres and causes their lubrication properties to significantly decrease. Utility Model Content

[0003] The technical problem to be solved by the utility model is to provide a hydrogel microsphere preparation device, which solves the problem that hydrogel microspheres with highly uniform particle sizes cannot be prepared in the existing hydrogel microsphere preparation process, thereby resulting in poor lubrication performance of the gel microspheres.

[0004] In order to solve the above technical problems, the technical solution of the utility model is as follows:

[0005] The embodiment of the utility model provides a hydrogel microsphere preparation device, comprising:

[0006] Reaction chamber;

[0007] A microporous pipe disposed at the bottom of the reaction chamber and communicating with the reaction chamber;

[0008] a continuous phase delivery assembly in communication with the reaction chamber;

[0009] a dispersed phase delivery assembly connected to the reaction chamber via a microporous conduit;

[0010] When in use, the continuous phase delivery component delivers the continuous phase to the reaction chamber, and the dispersed phase delivery component evenly divides the dispersed phase through the microporous pipeline and delivers the divided dispersed phase to the reaction chamber to react with the continuous phase.

[0011] Optionally, the hydrogel microsphere preparation device further comprises:

[0012] A stirring assembly is arranged on the reaction chamber.

[0013] Optionally, the stirring assembly includes:

[0014] A motor group disposed on the reaction chamber;

[0015] A connecting rod connected to the output end of the motor group, one end of the connecting rod is located in the reaction chamber;

[0016] A stirrer is disposed in the reaction chamber and connected to the end of the connecting rod, and the stirrer is disposed close to the microporous pipe.

[0017] Optionally, the microporous tubing includes:

[0018] A pipeline body and a microporous dividing net arranged on the top of the pipeline body;

[0019] Wherein, the bottom of the pipeline body is in a sealed state, and the top of the pipeline body is in an open state;

[0020] The top of the pipeline body is in sealed communication with the bottom of the reaction chamber.

[0021] Optionally, the pore size of the microporous segmentation mesh ranges from 50 microns to 500 microns.

[0022] Optionally, the continuous phase transport component comprises:

[0023] A continuous phase injection pipeline communicated with the top of the reaction chamber and a continuous phase injection container connected to the continuous phase injection pipeline.

[0024] Optionally, the dispersed phase transport component comprises:

[0025] a dispersed phase injection pipeline connected to the side of the microporous pipeline;

[0026] A dispersed phase injection container connected to the dispersed phase injection pipeline.

[0027] Optionally, the dispersed phase transport component further comprises:

[0028] A microinjection pump, wherein the dispersed phase injection container is arranged on the microinjection pump and is communicated with the input end of the microinjection pump, and the output end of the microinjection pump is communicated with the dispersed phase injection pipeline;

[0029] When in use, the microinjection pump injects the dispersed phase into the container and transports the dispersed phase to the microporous pipeline through the dispersed phase injection pipeline for segmentation.

[0030] Optionally, the dispersed phase injection pipeline is a soft capillary material tube for conveying liquid.

[0031] Optionally, scale lines are provided on the outside of the reaction chamber, and the zero scale line of the scale lines is located at the same horizontal line as the top and bottom surfaces of the microporous pipe.

[0032] The above solution of the utility model includes at least the following beneficial effects:

[0033] The hydrogel microsphere preparation device described in the utility model includes: a reaction chamber; a microporous pipe arranged at the bottom of the reaction chamber and connected to the reaction chamber; a continuous phase conveying component connected to the reaction chamber; a dispersed phase conveying component connected to the reaction chamber through a microporous pipe; when in use, the continuous phase conveying component conveys the continuous phase to the reaction chamber, and the dispersed phase conveying component evenly divides the dispersed phase through the microporous pipe, and conveys the divided dispersed phase to the reaction chamber to react with the continuous phase. Rapid preparation of hydrogel microspheres is achieved, while ensuring the high uniformity of the particle size of the hydrogel microspheres, thereby improving the lubricity of the hydrogel microspheres. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a structural schematic diagram of the hydrogel microsphere preparation device of the utility model;

[0035] Figure 2 It is a structural schematic diagram of the hydrogel microsphere preparation device of the utility model without an automatic opening and closing door;

[0036] Figure 3 It is a top view of the microporous pipeline of the hydrogel microsphere preparation device of the utility model.

[0037] Description of reference numerals:

[0038] Among them, 1. reaction chamber; 2. microporous pipeline; 21. pipeline body; 22. microporous partition net; 3. continuous phase conveying component; 31. continuous phase injection pipeline; 32. continuous phase injection container; 4. dispersed phase conveying component; 41. dispersed phase injection pipeline; 42. dispersed phase injection container; 43. micro injection pump; 5. stirring component; 51. connecting rod; 52. motor unit; 53. stirrer; 6. automatic opening and closing door. DETAILED DESCRIPTION

[0039] The exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0040] like Figures 1 to 3 As shown, the embodiment of the utility model provides a hydrogel microsphere preparation device, comprising:

[0041] Reaction chamber 1;

[0042] A microporous pipe 2 disposed at the bottom of the reaction chamber 1 and communicating with the reaction chamber 1;

[0043] A continuous phase transport component 3 communicated with the reaction chamber 1;

[0044] A dispersed phase transport component 4 connected to the reaction chamber 1 through a microporous pipe 2;

[0045] When in use, the continuous phase delivery component 3 delivers the continuous phase to the reaction chamber 1, and the dispersed phase delivery component 4 evenly divides the dispersed phase through the microporous pipe 2, and delivers the divided dispersed phase to the reaction chamber 1 to react with the continuous phase.

[0046] A stirring assembly 5 is arranged on the reaction chamber 1 .

[0047] In this embodiment, the dispersed phase is a precursor of chitosan hydrogel, silk fibroin hydrogel, and sodium hyaluronate hydrogel; the continuous phase is an oily substance, such as soybean oil, peanut oil, avocado oil, paraffin oil, olive oil, etc.; In this embodiment, the hydrogel microsphere preparation device realizes uniform segmentation of the dispersed phase before the reaction through the design of the microporous pipeline 2 and the dispersed phase conveying component 4, thereby ensuring the size of the dispersed phase during the reaction process, thereby ensuring the uniformity of the hydrogel microspheres; the design of the reaction chamber 1, the stirring component 5, and the phase conveying component 3 ensures that the dispersed phase and The continuous phase fully reacts, and when the dispersed phase is integrated into the continuous phase, it reacts uniformly under the action of external temperature. After the dispersed phase and the continuous phase fully react, the product after the reaction only needs to be repeatedly degreased, washed with water and filtered to obtain hydrogel microspheres with uniform particle size; thereby solving the problem that hydrogel microspheres with highly uniform particle size cannot be prepared in the existing hydrogel microsphere preparation process, resulting in poor lubrication performance of the gel microspheres, and the like; the rapid preparation of hydrogel microspheres is achieved, while ensuring the high uniformity of the particle size of the hydrogel microspheres, thereby improving the lubricity of the hydrogel microspheres.

[0048] In an optional embodiment of the present utility model, the stirring assembly 5 includes:

[0049] A motor group 52 disposed on the reaction chamber 1;

[0050] A connecting rod 51 connected to the output end of the motor group 52, one end of the connecting rod 51 is located in the reaction chamber 1;

[0051] An agitator 53 is disposed in the reaction chamber 1 and connected to the end of the connecting rod 51 . The agitator 53 is disposed close to the microporous pipe 2 .

[0052] In this embodiment, the motor group 52 is used to provide power, and the connecting rod 51 is used to drive the agitator 53 to rotate, and the rotation speed is between 200 and 1600 rpm (revolutions per minute); the agitator 53 stirs the dispersed phase and the continuous phase, thereby ensuring that the dispersed phase and the continuous phase are evenly mixed under the stirring centrifugal action; at the same time, the agitator 53 is also used to intercept the dispersed phase when the dispersed phase enters the reaction chamber 1 through the microporous pipe 2 to form uniform-sized microspheres; specifically, when the dispersed phase passes through the microporous pipe 2, it will be diverted into uniformly dispersed microchannels, and then intercepted into uniform-sized microspheres under the action of the rotating agitator 53, and integrated into the continuous phase; therefore, the agitator 53 needs to be set close to the microporous pipe 2 and located at the top of the microporous pipe 2.

[0053] In an optional embodiment of the present invention, the microporous pipeline 2 comprises:

[0054] A pipe body 21 and a microporous dividing net 22 arranged on the top of the pipe body 21;

[0055] The bottom of the pipe body 21 is in a sealed state, and the top of the pipe body 21 is in an open state;

[0056] The top of the pipe body 21 is sealed and communicated with the bottom of the reaction chamber 1 .

[0057] In this embodiment, the surface of the microporous dividing net 22 is uniform and fine micro-channels, which are used to divide the dispersed phase into uniformly dispersed micro-channels; the top of the pipe body 21 is provided with an external thread, and the bottom of the reaction chamber 1 is provided with an internal thread, and the top of the pipe body 21 and the reaction chamber 1 are screwed together by internal and external threads; the detachable setting of the microporous pipe 2 and the reaction chamber 1 is convenient for installation, and the pore size of the microporous dividing net 22 can be adjusted; the pore size range of the microporous dividing net 22 is 50 microns to 500 microns, and different microporous pipes 2 can be replaced according to the particle size range.

[0058] In an optional embodiment of the present utility model, the continuous phase conveying component 3 comprises:

[0059] A continuous phase injection pipe 31 communicated with the top of the reaction chamber 1 and a continuous phase injection container 32 connected to the continuous phase injection pipe 31, wherein the continuous phase injection container 32 is used to store the continuous phase and the continuous phase injection pipe 31 is used to transport the continuous phase.

[0060] In an optional embodiment of the present utility model, the dispersed phase conveying component 4 includes:

[0061] A dispersed phase injection pipe 41 connected to the side of the microporous pipe 2;

[0062] A dispersed phase injection container 42 connected to the dispersed phase injection pipeline 41 .

[0063] A microinjection pump 43, wherein the dispersed phase injection container 42 is disposed on the microinjection pump 43 and communicated with the input end of the microinjection pump 43, and the output end of the microinjection pump 43 is communicated with the dispersed phase injection pipeline 41;

[0064] When in use, the microinjection pump 43 injects the dispersed phase in the dispersed phase injection container 42 and transports the dispersed phase to the microporous pipe 2 through the dispersed phase injection pipe 41 for segmentation.

[0065] In this embodiment, the dispersed phase injection container 42 is used to store the dispersed phase and can be a syringe; the dispersed phase injection pipeline 41 is used to transport the dispersed phase; the microinjection pump 43 is used to uniformly transport the dispersed phase in the dispersed phase injection container 42 to the microporous pipeline 2, and uniformly form a dispersed microchannel through the microporous dividing net 22; the rotation speed of the microinjection pump 43 is adjustable, and the rotation speed range should be 1 to 30 ml per minute.

[0066] In an optional embodiment of the present invention, scale lines are provided on the outside of the reaction chamber 1, and the zero scale line of the scale lines is located at the same horizontal line as the top and bottom surfaces of the microporous pipe 2, and the setting of the scale lines facilitates the control of the input amount of the dispersed phase and the continuous phase.

[0067] In an optional embodiment of the present invention, the dispersed phase injection pipeline 41 is a soft capillary material tube for conveying liquid.

[0068] In an optional embodiment of the utility model, the hydrogel microsphere preparation device further includes:

[0069] An automatic opening and closing door 6 disposed between the reaction chamber 1 and the microporous pipe 2;

[0070] Wherein, the upper bottom surface of the automatic opening and closing door 6 and the zero scale line of the scale line are located at the same horizontal line;

[0071] When in use, when the dispersed phase conveying component 4 divides the dispersed phase through the microporous pipe 2, the automatic opening and closing door 6 opens to allow the divided dispersed phase to enter and enter the reaction chamber 1. When the dispersed phase conveying component 4 completes the conveying, the automatic opening and closing door 6 closes to ensure that the divided dispersed phase and the continuous phase can fully react in the reaction chamber 1. This design is mainly used to prevent the dispersed phase and the continuous phase from returning to the microporous pipe 2 during the reaction.

[0072] The specific process of the hydrogel microsphere preparation device described in the utility model is:

[0073] In this embodiment, silk fibroin is used as a raw material to prepare silk fibroin hydrogel microspheres, specifically as follows: 4 grams of silk is weighed for use, 20 milliliters of 6 to 9 mol / L lithium bromide solution is prepared, and then the silk is dissolved in the lithium bromide solution at high temperature to obtain a silk fibroin solution, and then 1 milliliter of a chemical cross-linking agent glycerol ether is added to the dispersed phase of the precursor solution to obtain a silk fibroin hydrogel precursor solution, which is placed in a refrigerator for use; the low-temperature silk fibroin precursor solution is added to the dispersed phase injection container 42, and then placed on a microinjection pump 43, and the injection speed is set to 1 milliliters per minute; prepare 400 milliliters of soybean oil, inject it into the reaction box through the continuous phase injection pipe 31, set the stirring speed to 800 revolutions per minute, then load the microporous pipe 2, ensure that each link is sealed and leak-proof, turn on the microinjection pump 43 switch, and prepare; after the injection is completed, take out the mixed reaction solution, incubate it in a 60°C oven with stirring overnight, and the next day, wash the reaction system with n-hexane to remove the oil phase, wash and filter with water to obtain hydrogel microspheres with uniform particle size; the silk fibroin hydrogel microspheres with a particle size of 227±14 microns can be obtained under this preparation.

[0074] The hydrogel microsphere preparation device described in the utility model can flexibly and efficiently obtain hydrogel microspheres with uniform particle sizes by controlling the fluid caliber size, injection speed and stirring speed when the dispersed phase is input. The device is detachable and different structural units can be replaced according to actual needs to meet the microsphere conditions. The device has a simple construction structure, is convenient and efficient, has low cost, and is easy to promote.

[0075] The above is a preferred embodiment of the present invention. It should be pointed out that, for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A hydrogel microsphere preparation device, characterized in that: include: Reaction chamber (1); A microporous pipe (2) disposed at the bottom of the reaction chamber (1) and communicating with the reaction chamber (1); A continuous phase transport component (3) in communication with the reaction chamber (1); A dispersed phase transport component (4) connected to the reaction chamber (1) via a microporous pipeline (2); When in use, the continuous phase delivery component (3) delivers the continuous phase to the reaction chamber (1), and the dispersed phase delivery component (4) evenly divides the dispersed phase through the microporous pipe (2) and delivers the divided dispersed phase to the reaction chamber (1) to react with the continuous phase.

2. The hydrogel microsphere preparation device according to claim 1, characterized in that: Also includes: A stirring assembly (5) is arranged on the reaction chamber (1).

3. The hydrogel microsphere preparation device according to claim 2, characterized in that: The stirring assembly (5) comprises: A motor group (52) arranged on the reaction chamber (1); a connecting rod (51) connected to the output end of the motor group (52), one end of the connecting rod (51) being located in the reaction chamber (1); A stirrer (53) is arranged in the reaction chamber (1) and connected to the end of the connecting rod (51), and the stirrer (53) is arranged close to the microporous pipe (2).

4. The hydrogel microsphere preparation device according to claim 1, characterized in that: The microporous pipeline (2) comprises: A pipeline body (21) and a microporous dividing net (22) arranged on the top of the pipeline body (21); The bottom of the pipeline body (21) is in a sealed state, and the top of the pipeline body (21) is in an open state; The top of the pipeline body (21) is in sealed communication with the bottom of the reaction chamber (1).

5. The hydrogel microsphere preparation device according to claim 4, characterized in that: The pore size of the microporous dividing net (22) ranges from 50 microns to 500 microns.

6. The hydrogel microsphere preparation device according to claim 1, characterized in that: The continuous phase transport component (3) comprises: A continuous phase injection pipe (31) communicated with the top of the reaction chamber (1) and a continuous phase injection container (32) connected to the continuous phase injection pipe (31).

7. The hydrogel microsphere preparation device according to claim 1, characterized in that: The dispersed phase transport component (4) comprises: a dispersed phase injection pipeline (41) connected to the side of the microporous pipeline (2); A dispersed phase injection container (42) connected to the dispersed phase injection pipeline (41).

8. The hydrogel microsphere preparation device according to claim 7, characterized in that: The dispersed phase transport component (4) further comprises: A microinjection pump (43), wherein the dispersed phase injection container (42) is arranged on the microinjection pump (43) and is connected to the input end of the microinjection pump (43), and the output end of the microinjection pump (43) is connected to the dispersed phase injection pipeline (41); When in use, the microinjection pump (43) injects the dispersed phase into the dispersed phase container (42) and transports the dispersed phase into the microporous pipe (2) through the dispersed phase injection pipe (41) for segmentation.

9. The hydrogel microsphere preparation device according to claim 7, characterized in that: The dispersed phase injection pipeline (41) is a soft capillary material tube used for conveying liquid.

10. The hydrogel microsphere preparation device according to claim 1, characterized in that: The reaction chamber (1) is provided with scale lines on the outside, and the zero scale line of the scale lines is located at the same horizontal line as the top and bottom surfaces of the microporous pipe (2).

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