Liquid injection and screening conveying device in PCR (Polymerase Chain Reaction) experiment

Abstract

The utility model discloses a liquid injection and screening conveying device in a PCR (Polymerase Chain Reaction) experiment, which comprises a workbench and a conveyor belt, and further comprises a test tube disc driven by the conveyor belt and a plurality of test tubes inserted on the test tube disc; the liquid injection device is erected in the middle of the conveying belt, and a plurality of pipettes for injecting liquid into the test tubes are arranged on the liquid injection device; the clamping mechanism is arranged on the conveying belt in a sliding manner and comprises a partition plate for pushing the first end of the test tube disc and a clamping plate for clamping the second end of the test tube disc. According to the liquid injection and screening conveying device in the PCR experiment, the liquid injection device is arranged to inject liquid into test tubes, and the test tube disc is oscillated when moving in the conveying direction by virtue of the cooperation of the conveying belt and the side plates, so that cells in the test tubes are fully reacted with reaction liquid, and finally, waste liquid is sucked out by virtue of the liquid suction device, so that the whole extraction process is completed; and the related structure is relatively simple, so that the process is simplified, and continuous operation of a plurality of test tubes can be simultaneously completed.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid extraction devices, in particular to a liquid injection and sieving conveying device in a PCR experiment. Background Art

[0002] In conjunction with publication number CN207330950U, publication date 2018-05-08, a nucleic acid extraction instrument is disclosed.

[0003] In the prior art including the above-mentioned patent, a working platform, a working arm, a working arm driving assembly and a working arm transmission assembly are included, the working arm transmission assembly includes a first up and down transmission mechanism, a second up and down transmission mechanism and a horizontal transmission mechanism; the working arm includes an upper arm and a lower arm arranged up and down; the first motor is connected to and drives the first up and down transmission mechanism, the first up and down transmission mechanism is connected to the upper arm and drives the upper arm to move up and down; the second motor is connected to and drives the second up and down transmission mechanism, the second up and down transmission mechanism is connected to the lower arm; the third motor is connected to and drives the horizontal transmission mechanism, the horizontal transmission mechanism is connected to the upper arm and the lower arm. The above technology injects the sample containing nucleic acid into the first column of the deep-well plate which has been packaged with the nucleic acid extraction reagent, and then pushes the deep-well plate into the bottom of the fixed frame. The device will perform up and down movements and left and right movements according to the corresponding process until the nucleic acid extraction is completed, including the following steps: the magnetic sleeve provided on the lower arm is inserted into the first column and moves up and down at high speed, so that the cell wall is broken and the nucleic acid is lysed; then the magnetic rod is inserted into the magnetic sleeve, the nano-magnetic beads in the deep-well plate will adsorb the nucleic acid, the magnetic rod absorbs the nano-magnetic beads, the upper arm and the lower arm then move upward and move right to the second column, the upper arm and the lower arm slowly move down to the deep-well plate together, and then the upper arm quickly rises to the top, and the lower arm moves up and down to wash the nucleic acid; then it is transferred to the third column, and after completion, it is transferred to the fourth column for washing, and then transferred to the fifth column, and the nucleic acid is eluted from the magnetic beads in the fifth column. However, since the up, down, left and right movements of the working arm are realized by a transmission mechanism, and the transmission mechanism is composed of multiple transmission wheels and gears, and is driven by a motor, when performing extraction and oscillation operations, the transmission process is relatively cumbersome, which is not conducive to large-scale sample testing. Utility Model Content

[0004] The utility model aims to provide a liquid injection and sieving conveying device in PCR experiment, which is used to solve the above problems.

[0005] In order to achieve the above-mentioned purpose, the utility model provides the following technical solutions: a liquid injection and sieving conveying device in a PCR experiment, comprising a workbench and a conveyor belt, including:

[0006] A test tube tray driven by a conveyor belt, on which a plurality of test tubes are inserted;

[0007] A liquid injection device mounted in the middle of the conveyor belt, on which a plurality of pipettes for injecting liquid into the test tubes are arranged;

[0008] A clamping mechanism slidably disposed on the conveyor belt, comprising a partition plate for pushing against the first end of the test tube tray and a clamping plate for clamping the second end of the test tube tray, and in the clamping state, the test tube tray is in a horizontal state;

[0009] A side plate fixedly arranged on the side of the conveyor belt has a wave-shaped convex strip on its inner wall that abuts against the partition plate and the clamping plate;

[0010] A liquid suction device is arranged at the end of the conveyor belt and moves in the vertical direction.

[0011] Preferably, it further comprises a guide rail disposed on the workbench and located at the end of the conveyor belt, and blocks slidably assembled with the guide rail are provided on both sides of the test tube tray.

[0012] Preferably, the clamping plate is provided with an arc-shaped spring piece, and the arc-shaped spring piece is provided with a clamping groove for accommodating the clamping block.

[0013] Preferably, the method further comprises a magnetic frame arranged on the workbench and located in the extending direction of the conveyor belt, on which a magnetic part for attracting magnetic beads in the test tube is provided, and the liquid aspiration device is located directly above the magnetic frame.

[0014] Preferably, the number of the side panels is two, and the crests and troughs of two wave-shaped convex strips on opposite sides are staggered.

[0015] Preferably, elastic members are provided on both sides of the partition plate and the clamping plate.

[0016] In the above technical scheme, the utility model provides a liquid injection and sieving conveying device in a PCR experiment, which has the following beneficial effects: the test tube is injected with liquid by providing a liquid injection device, and the cooperation of the conveyor belt and the side plate is used to make the test tube disk vibrate when moving along the conveying direction, thereby prompting the cells and reaction liquid in the test tube to fully react, and then the test tube disk is removed for centrifugation, and finally the waste liquid is sucked out by the liquid suction device to complete the entire extraction process, and the structure involved is relatively simple, which simplifies the process and can complete the continuous operation of multiple test tubes at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present utility model. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0018] Figure 1 An overall three-dimensional schematic diagram provided for an embodiment of the utility model;

[0019] Figure 2A schematic diagram of the positions of the conveyor belt, partition plate and snap-in plate provided in the embodiment of the utility model;

[0020] Figure 3 The embodiment of the utility model provides Figure 2 Enlarged schematic diagram of the middle arc shrapnel;

[0021] Figure 4 A schematic diagram of the magnetic frame structure provided in an embodiment of the utility model.

[0022] Description of reference numerals:

[0023] 1. Conveyor belt; 2. Liquid injection device; 4. Liquid suction device; 5. Test tube tray; 51. Card block; 6. Partition plate; 7. Card plate; 8. Side plate; 9. Guide rail; 10. Magnetic frame; 11. Magnetic part; 12. Elastic part; 13. Arc spring piece; 14. Card slot. DETAILED DESCRIPTION

[0024] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0025] like Figure 1-4 As shown, a liquid injection and sieving conveying device in a PCR experiment includes a workbench and a conveyor belt 1, including:

[0026] A test tube tray 5 driven by the conveyor belt 1, on which a plurality of test tubes are inserted;

[0027] A liquid injection device 2 is mounted in the middle of the conveyor belt 1, on which a plurality of pipettes for injecting liquid into the test tubes are arranged;

[0028] A clamping mechanism slidably disposed on the conveyor belt 1 comprises a partition plate 6 for pushing against the first end of the test tube tray 5 and a clamping plate 7 for clamping the second end of the test tube tray 5, and in the clamping state, the test tube tray 5 is in a horizontal state;

[0029] A side plate 8 fixedly arranged on the side of the conveyor belt 1 has a wave-shaped convex strip on its inner wall that abuts against the partition plate 6 and the clamping plate 7;

[0030] A liquid suction device 4 is arranged at the end of the conveyor belt 1 and moves in the vertical direction.

[0031] Specifically, the test tube tray 5 can be a multi-row comb-tooth structure or a single-row structure. The specific number of rows can be adjusted according to actual needs, and the test tubes are linearly inserted into the test tube tray 5. The first end of the test tube tray 5 is placed on the partition 6, and the second end is clamped with the clamping plate 7. The conveyor belt 1 is started to drive the partition 6 and the clamping plate 7 to move, and the test tube tray 5 is driven to move to the bottom of the injection device 2. The lysis solution and magnetic beads are injected into the whole row of test tubes at the same time through the pipette. Then the conveyor belt drives the test tube tray 5 to move, and the next row of test tubes is injected. The wavy convex strips on the side plate 8 are always in contact with the partition The plate 6 and the clamping plate 7 are against each other, and during the movement, the partition plate 6 and the clamping plate 7 are pushed and moved, so that the test tube tray 5 generates oscillation when moving along the conveying direction, thereby prompting the cells and reaction liquid in the test tube to fully react. When the test tube tray 5 is conveyed to the end of the conveyor belt 1, the staff will take the test tube tray 5 away and place it in the centrifuge for centrifugation, and clamp the centrifuged test tube and the test tube tray 5 again through the clamping mechanism, and suck out the waste liquid through the suction device 4 at the end of the conveyor belt 1 for purification, forming a work flow of injection-oscillation-centrifugation-suction.

[0032] In the above technology, the test tube is injected with liquid by providing a liquid injection device 2, and the cooperation of the conveyor belt 1 and the side plate 8 is utilized to make the test tube disk 5 vibrate when moving along the conveying direction, thereby prompting the cells and the reaction liquid in the test tube to fully react, and then the test tube disk 5 is removed for centrifugation, and finally the waste liquid is sucked out by the liquid suction device 4 to complete the whole extraction process, and the structure involved is relatively simple, so that the process is simplified, and continuous operation of multiple test tubes can be completed at the same time.

[0033] As an embodiment further provided by the present invention, it also includes a guide rail 9 disposed on the workbench and located at the end of the conveyor belt 1 , and blocks 51 slidably assembled with the guide rail 9 are disposed on both sides of the test tube tray 5 .

[0034] Specifically, a reversing roller is provided at the end of the conveyor belt 1 to enable the conveyor belt 1 to return in the opposite direction, and the test tube tray 5 is clamped again on the clamping mechanism at the end after centrifugation, and then the clamping plate 7 moves to the reversing roller and changes from a linear stroke to a circular stroke. During the switching process, the clamping plate 7 is disengaged from the test tube tray 5 again, which will cause the clamping block 51 to slide and assemble with the guide rail 9, while the partition 6 maintains the resistance to the test tube tray 5, and slides the test tube tray 5 along the guide rail 9, so that the centrifuged test tube tray 5 automatically disengages from the conveyor belt 1 and moves to directly below the liquid aspiration device 4, and there is no need for personnel to remove the test tube tray 5 from the conveyor belt 1.

[0035] As another embodiment further provided by the present invention, an arc-shaped spring piece 13 is provided on the clamping plate 7 , and a clamping groove 14 for accommodating the clamping block 51 is provided on the arc-shaped spring piece 13 .

[0036] Specifically, the arc-shaped spring piece 13 has a certain elasticity. When the test tube tray 5 is clamped, the second end needs to be aligned with the clamping plate 7 and then pressed down to squeeze the clamping block 51 into the clamping groove 14. At this time, the arc-shaped spring piece 13 and the clamping block 51 press against each other, so that the test tube tray 5 is clamped, ensuring that the test tube tray 5 is always fixed by the clamping plate 7 during the movement. When the clamping plate 7 moves onto the reversing roller, the clamping plate 7 will move in a circle with the reversing roller. At this time, the arc-shaped spring piece 13 will rotate relative to the test tube tray 5, so that the clamping block 51 is disengaged from the clamping groove 14 and squeezes the arc-shaped spring piece 13, so that the test tube tray 5 is disengaged, and the clamping process is relatively simple.

[0037] As another embodiment further provided by the present invention, it also includes a magnetic frame 10 arranged on the workbench and located in the extension direction of the conveyor belt 1, on which a magnetic part 11 for attracting magnetic beads in the test tube is provided, and the liquid pipetting device 4 is located directly above the magnetic frame 10.

[0038] Specifically, the magnetic parts 11 are arranged in groups of two corresponding to the teeth of the test tube tray 5. After the test tube tray 5 is slidably assembled with the guide rail 9, the test tube tray 5 slides with the upper side of the magnetic rack 10. At this time, the test tube is located between the two magnetic parts 11. The magnetic beads in the test tube are absorbed by the magnetic part 11, and then the liquid aspiration device 4 is started to absorb the inside of the entire row of test tubes directly below. After completion, the partition 6 pushes the test tube tray 5 to continue moving forward for the next round of suction. After all the test tubes in the test tube tray 5 have completed the suction, the next test tube tray 5 continues to slide into the guide rail 9.

[0039] As another embodiment further provided by the present invention, there are two side panels 8, and the crests and troughs of two wave-shaped convex strips on opposite sides are staggered.

[0040] Specifically, the distance between the wavy convex strips on both sides is always consistent, and the partition 6 and the clamping plate 7 on both sides reciprocate in the vertical direction during the transmission process, thereby stabilizing the path of the test tube disk 5 and improving the oscillation effect of the test tube disk 5. The elastic parts 12 provided on both sides of the partition 6 and the clamping plate 7 can reduce the squeezing of the wavy convex strips on the partition 6 and the clamping plate 7 to avoid damage, and at the same time, the oscillation effect is further amplified by the deformation of the elastic parts 12 themselves.

[0041] Working principle: Place the first end of the test tube tray 5 on the partition 6, and the second end is clamped with the clamping plate 7. The second end needs to be aligned with the clamping plate 7 and then pressed down to squeeze the clamping block 51 into the clamping groove 14. At this time, the arc-shaped spring piece 13 and the clamping block 51 press against each other, so that the test tube tray 5 is clamped, ensuring that the test tube tray 5 is always fixed by the clamping plate 7 during the movement. Start the conveyor belt 1 to drive the partition 6 and the clamping plate 7 to move, and drive the test tube tray 5 to move to the bottom of the injection device 2, and inject the lysis solution and magnetic beads into the whole row of test tubes at the same time through the pipette. The rear conveyor belt 1 drives the test tube tray 5 to move and inject liquid into the next row of test tubes. The distance between the wavy convex strips on both sides is always consistent. During the conveying process, the partition plates 6 and the clamping plates 7 are clamped from both sides to reciprocate in the vertical direction, thereby driving the test tube tray 5 to oscillate. The elastic members 12 provided on both sides of the partition plates 6 and the clamping plates 7 can slow down the squeezing of the wavy convex strips on the partition plates 6 and the clamping plates 7 to avoid damage. At the same time, the deformation of the elastic members 12 themselves further amplifies the oscillation effect. The test tube tray 5 is driven by the conveyor belt 1 to After the test tube tray 5 is loaded, the staff takes away the test tube tray 5 and places it in the centrifuge for centrifugal operation, and clamps the centrifuged test tube and the test tube tray 5 again through the clamping mechanism, then the clamping plate 7 moves to the reversing roller and changes from a linear stroke to a circular stroke. At this time, the arc spring piece 13 rotates relative to the test tube tray 5, so that the clamping block 51 disengages from the clamping groove 14 and squeezes the arc spring piece 13, so that the test tube tray 5 is disengaged, and the clamping block 51 is slidably assembled with the guide rail 9, while the partition plate 6 keeps pushing the test tube tray 5, and the centrifuged test tube tray 5 is Sliding along the guide rail 9, the test tube tray 5 automatically detaches from the conveyor belt 1 and is slidably assembled with the guide rail 9. The test tube tray 5 slides with the upper side of the magnetic rack 10. At this time, the test tube is located between the two magnetic parts 11, and the magnetic beads in the test tube are sucked through the magnetic part 11. Then the liquid aspiration device 4 is started to suck the inside of the entire row of test tubes directly below. After completion, the partition 6 pushes the test tube tray 5 to continue moving forward for the next round of suction. After all test tubes in the test tube tray 5 have completed the suction, the next test tube tray 5 continues to slide into the guide rail 9.

[0042] The above only describes some exemplary embodiments of the present invention by way of illustration. It is undoubted that those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A liquid injection and sieving conveying device in a PCR experiment, comprising a workbench and a conveyor belt (1), characterized in that: include: A test tube tray (5) driven by a conveyor belt (1) and having a plurality of test tubes inserted thereon; A liquid injection device (2) mounted in the middle of the conveyor belt (1) and provided with a plurality of pipettes for injecting liquid into the test tubes; A clamping mechanism slidably arranged on the conveyor belt (1), comprising a partition plate (6) for pushing against the first end of the test tube tray (5) and a clamping plate (7) for clamping the second end of the test tube tray (5), and in the clamped state, the test tube tray (5) is in a horizontal state; A side plate (8) fixedly arranged on the side of the conveyor belt (1) has an inner wall provided with a wave-shaped convex strip that abuts against the partition plate (6) and the clamping plate (7); A liquid suction device (4) is arranged at the end of a conveyor belt (1) and moves in a vertical direction.

2. The device for liquid injection and sieving in PCR experiment according to claim 1, characterized in that: It also comprises a guide rail (9) arranged on the workbench and located at the end of the conveyor belt (1), and both sides of the test tube tray (5) are provided with a clamping block (51) which is slidably assembled with the guide rail (9).

3. The device for liquid injection and sieving in PCR experiment according to claim 2, characterized in that: The clamping plate (7) is provided with an arc-shaped spring piece (13), and the arc-shaped spring piece (13) is provided with a clamping groove (14) for accommodating a clamping block (51).

4. The device for liquid injection and sieving in PCR experiment according to claim 1, characterized in that: It also includes a magnetic frame (10) disposed on the workbench and located in the extending direction of the conveyor belt (1), on which a magnetic part (11) for attracting magnetic beads in the test tube is provided, and the liquid aspiration device (4) is located directly above the magnetic frame (10).

5. The device for liquid injection and sieving in PCR experiment according to claim 1, characterized in that: The number of the side plates (8) is two, and the crests and troughs of the two wave-shaped convex strips on opposite sides are staggered.

6. The device for liquid injection and sieving in PCR experiment according to claim 1, characterized in that: Elastic parts (12) are provided on both sides of the partition plate (6) and the clamping plate (7).

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