Two-dimensional thin-layer chromatography-small portable mass spectrum separation and detection device

Abstract

The utility model discloses a two-dimensional thin-layer chromatography-small portable mass spectrum separation and detection device which comprises a small portable mass spectrometer (2) and an aluminum-based silica gel thin-layer chromatographic plate (1) which are connected through an alligator clip (4), and a sample (3) is arranged on the aluminum-based silica gel thin-layer chromatographic plate (1). According to an analysis method adopting the device disclosed by the utility model, the combination of the aluminum-based silica gel thin-layer chromatographic plate and the in-situ spray ionization is an efficient and powerful method, and the advantages of the aluminum-based silica gel thin-layer chromatographic plate and the in-situ spray ionization can be fully exerted, so that a more powerful analysis tool is provided for the field of inspection and detection.

Description

Technical Field

[0001] The utility model relates to a separation detection device, in particular to a two-dimensional thin layer chromatography-small portable mass spectrometry separation detection device. Background Art

[0002] Point-of-care testing (POCT) is a method for rapid on-site analysis that provides rapid biochemical test results, reduces delays in clinical decision-making, eliminates the need to transport and prepare clinical samples, and helps healthcare professionals make immediate diagnoses or clinical interventions. POCT methods include tests such as urine pregnancy tests and home blood glucose meters, which involve smearing samples (such as urine, blood) onto a substrate to trigger a reaction to produce a test result, which is the simplest form of quantification; POCT biosensors are often used in POCT devices, where biological components are attached to a solid surface and interact with the analyte, and these interactions can be detected by electrochemical or optical methods for clinical testing; benchtop POCT analyzers are used, including spectrophotometric substrate and enzyme activity measurements, hematological particle counting, immunoassays, and sensor-based blood gas analysis. Mass spectrometry technology has great potential in the field of POCT, and related reported applications include drug monitoring, clinical metabolomics, protein biomarker detection, toxicology screening, etc.

[0003] Mass spectrometers have the advantages of high throughput, high sensitivity and high accuracy in analytical measurement. However, the size and weight of large laboratory mass spectrometers hinder their mobility and cannot meet the needs of rapid clinical testing. At the same time, the complexity of mass spectrometry analysis methods and the time-consuming sample preparation are also important factors restricting the clinical application of large laboratory mass spectrometers. Utility Model Content

[0004] The technical problem to be solved by the utility model is to provide a two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device.

[0005] The utility model discloses a two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device, which comprises a small portable mass spectrometer and an aluminum-based silica gel thin layer chromatography plate connected by an alligator clip, wherein a sample is included on the aluminum-based silica gel thin layer chromatography plate.

[0006] The two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device described in the utility model further comprises a chromatography cylinder, and the chromatography cylinder comprises a chromatography liquid and a filter paper.

[0007] The utility model discloses a two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device, wherein the aluminum-based silica gel thin layer chromatography plate is cut into equilateral triangles by using an embossing device.

[0008] The two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device described in the utility model, wherein the crocodile clip is made of copper, the aluminum-based silica gel thin layer chromatography plate is fixed to the front end 5 mm of the discontinuous atmospheric pressure injection interface of the small portable mass spectrometer by the crocodile clip, and the rear end of the crocodile clip is connected to the small portable mass spectrometer through a wire.

[0009] The two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device described in the utility model, wherein the mass spectrometer is a Miniβ small portable mass spectrometer.

[0010] The utility model discloses a two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device, wherein the device also includes a glass spotting capillary, a dyeing liquid, an electric hair dryer and a spray solvent.

[0011] The utility model two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device is different from the prior art in that:

[0012] The combination of the aluminum-based silica gel thin layer chromatography plate and the in-situ spray ionization in the utility model device is an efficient and powerful method, which can give full play to the advantages of each, thereby providing a more powerful analytical tool for the inspection and detection field.

[0013] 1. The device of the utility model can be used for bedside online testing, reducing the delay in clinical decision-making and diagnosis and eliminating the need to transport and prepare clinical samples;

[0014] 2. The utility model device adopts a small portable mass spectrometer to eliminate the size and weight limitations of large laboratory mass spectrometers, and also solves the complexity and time-consuming nature of mass spectrometry methods to a certain extent;

[0015] 3. The method using the device of the utility model adopts the front-end treatment of thin layer chromatography, which can reduce the matrix effect to a certain extent compared with the paper matrix;

[0016] 4. The utility model adopts the method of using aluminum-based silica gel thin layer chromatography plate as the electrospray carrier, which is more convenient than scraping off the sample point for detection on the traditional glass silica gel chromatography plate.

[0017] The two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device of the present invention is further described below in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the structure of the two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device in the utility model;

[0019] Figure 2The following is a small portable mass spectrometer secondary mass spectrometer of three biomarkers and their internal standards in the embodiment of the utility model, wherein a, c, e represent γ-aminobutyric acid, 2-hydroxyglutaric acid and N-acetyl-L-aspartic acid, respectively; b, d, f represent γ-aminobutyric acid-d6, [ 2 H4]-2-hydroxyglutarate disodium salt and N-acetyl-L-aspartate-2,3,3-d3;

[0020] Figure 3 It is the response surface diagram of different variable combinations in the utility model;

[0021] The translations of all English words appearing in the drawings of this utility model are as follows:

[0022] Relative Abundance: relative abundance;

[0023] Normalized Intensity: Normalized intensity;

[0024] Amount of spray solvent: Amount of spray solvent used;

[0025] Voltage: voltage;

[0026] Distance: distance;

[0027] The Chinese translations of the English substance names are consistent with those in Table 1. DETAILED DESCRIPTION

[0028] 1. Instruments and Devices

[0029] Miniβ small portable mass spectrometer (55cm×24cm×31cm, Beijing Qingpu Technology Co., Ltd.); TLCsilica gel60F254 MS-grade aluminum-based silica gel thin layer chromatography plate (Sigma-Aldrich (Shanghai) Trading Co., Ltd.); glass spotting capillary (Beijing Ruizekang Technology Co., Ltd.); embossing device (Zhejiang Kamei Stationery Co., Ltd.); Milli-Q ultrapure water device (Millipore, USA); pipette tips used in the experiment (Bio-Rad, USA).

[0030] like Figure 1 As shown, the utility model provides a two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device, comprising a small portable mass spectrometer 2 and an aluminum-based silica gel thin layer chromatography plate 1 connected by an alligator clip 4, and a sample 3 is included on the aluminum-based silica gel thin layer chromatography plate 1.

[0031] The utility model also includes a chromatography cylinder, which includes a chromatography liquid and a filter paper. The aluminum-based silica gel thin layer chromatography plate 1 is cut into an equilateral triangle by an embossing device. The crocodile clip 4 is made of copper, and the aluminum-based silica gel thin layer chromatography plate 1 is fixed to the front end 5 mm of the discontinuous atmospheric pressure injection interface of the small portable mass spectrometer 2 by the crocodile clip 4, and the rear end of the crocodile clip 4 is connected to the small portable mass spectrometer 2 through a wire. The small portable mass spectrometer 2 is a Miniβ small portable mass spectrometer. The utility model device also includes a glass spotting capillary, a staining solution, a hair dryer and a spray solvent.

[0032] 2. Materials and Reagents

[0033] The standard substances of γ-aminobutyric acid, 2-hydroxyglutaric acid, and N-acetyl-L-aspartic acid used in the experiment are all commercially available standard samples of the highest purity, from Shanghai Yuanye Biotechnology Co., Ltd. The standard substances are prepared into 1000 mg / L standard stock solutions with methanol, and diluted with methanol to standard working solutions as needed when used; [ 2 Stable isotope standards of H4-2-hydroxyglutaric acid disodium salt, N-acetyl-L-aspartic acid-2,3,3-d3 and γ-aminobutyric acid-d6 were provided by Anpu Experimental Technology (Shanghai) Co., Ltd. and Guangdian Metrology and Testing Group Co., Ltd., respectively. Bromocresol green, nintriol, pyridine, n-butanol and sodium hydroxide were all from Beijing Inokai Technology Co., Ltd. The material information of the three biomarkers is shown in Table 1.

[0034] Table 1 Material information of three biomarkers

[0035]

[0036] 3. Experimental steps

[0037] The method for detecting three biomarkers of brain glioma using the device of the utility model comprises the following steps:

[0038] (A) Cut a 5 cm × 7.5 cm aluminum-based silica gel thin layer chromatography plate 1 into 2 cm × 7.5 cm strips;

[0039] Use a glass spotting capillary to draw the sample and spot it on an aluminum-based silica gel thin layer chromatography plate 1 cm from the bottom edge;

[0040] Put 2 mL of chromatography liquid in the chromatography cylinder, put in a piece of filter paper (the function of the filter paper is to saturate the steam in the chromatography cylinder so as to speed up the subsequent thin layer chromatography chromatography) and wait for the steam to be saturated; the chromatography liquid is n-butanol: formic acid: water in a volume ratio of 8:3:2;

[0041] After the steam is saturated, put the sample-spotted and dried aluminum-based silica gel thin layer chromatography plate 1 into it, and take it out when the front of the solution is 2 cm away from the top, and mark the front of the solution.

[0042] (B) Dry the aluminum-based silica gel thin layer chromatography plate 1 with a hair dryer and then immerse it in a staining solution for 1 second. Take it out and dry it with hot air at 110° C. to develop the color. Mark the location of sample 3 and determine the Rf values ​​of the three markers. The results are: N-acetyl-L-aspartic acid Rf=0.5, 2-hydroxyglutaric acid Rf=0.2 and γ-aminobutyric acid Rf=0.3;

[0043] (C) Repeat step (A) to obtain an aluminum-based silica gel thin layer chromatography plate 1 after chromatography, mark the location of the sample 3 on the unstained aluminum-based silica gel thin layer chromatography plate 1 according to the result of step (B), and cut it with a triangle embossing tool, so that the cut triangle aluminum-based silica gel thin layer chromatography plate 1 is an equilateral triangle;

[0044] (D) The triangular aluminum-based silica gel thin layer chromatography plate 1 is fixed to the front end 5 mm of the discontinuous atmospheric pressure injection interface with a copper alligator clip 4, and the rear end of the alligator clip 4 is connected to the Miniβ small portable mass spectrometer through a wire; the spray solvent is dropped onto the triangular aluminum-based silica gel thin layer chromatography plate 1, and mass spectrometry detection and analysis is performed; the spray solvent is 60% methanol aqueous solution (with 0.5% ammonia water added), and the amount is 7 μL. The parameters of mass spectrometry detection and analysis are shown in Table 2. Spray voltage 4250V; ionization mode is negative ion mode; collision gas is air; collision time is 500ms; average number of times is 2 times; continuous number of times is 3; injection time is 40ms; the mass-to-charge ratio of the injection low-end mass cutoff is m / z 50.

[0045] Table 2 Analysis parameters of small portable mass spectrometer for three biomarkers and their internal standards

[0046]

[0047] 4. Results and Discussion

[0048] The main body of the utility model device is an aluminum-based silica gel thin layer chromatography plate 1 fixed to the front end of the discontinuous atmospheric pressure injection interface of the Miniβ small portable mass spectrometer for spray ionization. The aluminum-based silica gel thin layer chromatography plate 1, which is easy to cut, is used to perform thin layer chromatography analysis on the sample to be tested, and the location of the target object is determined according to the ratio shift value (Rf) and cut with an embossing tool; then a copper mini alligator clip 4 is fixed to the front end of the Miniβ discontinuous atmospheric pressure injection interface, and the tail end of the alligator clip 4 is connected to the Miniβ with a wire to apply a high voltage power supply. Spray solvent is dripped on the cut aluminum-based silica gel thin layer chromatography plate 1 to perform spray ionization and mass spectrometry analysis.

[0049] (1) Selection of thin layer chromatography plates

[0050] Compared with the traditional paper spray method, this device cleverly eliminates a large number of impurities through one-dimensional thin layer chromatography separation. Three different coatings of thin layer chromatography plates were studied: cellulose, silica gel and alumina. Alumina coating is not suitable for the analysis of non-polar substances. It has strong adsorption for polar substances, but is limited to alkaline or neutral lipophilic compounds. Cellulose coating is poor in separating polar substances. Silica gel has high chemical stability, usually has a certain polarity, and is suitable for the analysis of a wide range of substances. In the study of three biomarkers of brain glioma, γ-aminobutyric acid (GABA), N-acetyl-L-aspartic acid (NAA) and 2-hydroxyglutaric acid (2-HG) have polar functional groups and belong to polar compounds. The silica gel coating is the most suitable. Taking into account the applicability, stability and tailorability of the coating, the aluminum-based silica gel thin layer chromatography plate was finally selected.

[0051] (2) Optimization of chromatography fluid

[0052] In addition to promoting the diffusion of samples on thin layer chromatography, the chromatographic fluid also has a significant impact on the efficiency and speed of chromatographic separation. Previous studies mainly used ternary eluent mixtures (e.g., n-butanol: acetic acid: water) for elution. Compared with acetic acid, formic acid can better provide acidic conditions, leading to amino protonation and ion pair formation, and enhancing the interaction with water. Therefore, the ternary mixture system of n-butanol: formic acid: water was studied in detail, and the component ratio was optimized for the three markers. Finally, their volume ratio was determined to be 8:3:2, and the elution system was applied to 2mL of eluent.

[0053] (3) Optimization of staining solution

[0054] It is necessary to select a suitable dyeing solution to determine the Rf values ​​of the three targets. The utility model uses two dyeing solutions to effectively dye GABA, 2-HG and NAA. Ninhydrin is an electrophilic reagent that can react with nucleophilic reagents, including ammonia, amines, enamines, ureas, amides, anilines, etc. The primary amine in GABA easily reacts with ninhydrin to form Heluman purple. The addition of pyridine enhances the formation of color, so we choose to dissolve ninhydrin in pyridine and acetone to obtain a GABA-specific dye solution. Bromocresol green is a negative ion dye of triphenylmethane, which presents a yellow single negative ion form at a lower pH value (acidic conditions) and a blue-green double negative ion under alkaline conditions. Adjusting the acidity with sodium hydroxide can specifically dye the carboxyl groups in NAA and 2-HG without heating and has a relatively long service life. Since immersion dyeing has the advantages of short time, reusable dyes, and immersion in a closed container to avoid environmental pollution, immersion dyeing is selected. Therefore, in order to perform efficient thin layer chromatography analysis, the thin layer chromatography plate after chromatography is immersed in a staining solution (ninhydrin solution or bromocresol green solution) for 1 second and dried with hot air at 110°C for color development.

[0055] The preparation method of ninhydrin solution is as follows: 1 g of ninhydrin is dissolved in 10 mL of pyridine and the volume is made up to 30 mL with acetone to prepare ninhydrin reagent.

[0056] The preparation method of bromocresol green solution is as follows: add 0.04g bromocresol green to 100mL anhydrous ethanol and titrate with 0.1mol / L sodium hydroxide aqueous solution until the solution turns blue.

[0057] The characteristics of the triangular aluminum-based silica gel thin layer chromatography plate tip, the type and amount of spray solvent, and the distance between the tip and the discontinuous atmospheric pressure injection interface will affect the peak intensity of the target substance.

[0058] (4) Optimization of the tip angle of aluminum-based silica gel thin layer chromatography plate 1

[0059] Changes in the tip angle of the aluminum-based silica gel thin layer chromatography plate 1 will affect the formation, stability and ionization efficiency of the spray droplets. The optimal tip angle is conducive to the formation of uniform, stable and tiny droplets, and enhances subsequent ionization and ion production. Studies on five tip angles (15°, 30°, 45°, 60°, and 75°) show that a smaller tip angle leads to uneven droplet distribution during the spray process, affecting the uniformity and efficiency of ions, resulting in reduced ion production and weakened signal intensity; as the angle increases, the signal intensity gradually increases; but at 75°, due to external interference and matrix impurities entering the small portable mass spectrometer 2, the noise signal increases and the signal intensity of the target substance decreases, resulting in experimental instability. The peak intensity of the target is the highest at 60°.

[0060] (5) Optimization of spray solvent

[0061] The selection and combination of spray solvents can significantly affect the signal intensity and stability of the small portable mass spectrometer 2. First, three solvents with different polarities, acetonitrile, water and methanol, were compared. In the comparison of the signal intensity of the three pure solvents, methanol had the highest intensity. Methanol has good volatility and solubility, which helps to form small and uniform droplets during the spray process, thereby improving the ionization efficiency of sample molecules and enhancing the intensity of mass spectrometry peaks. The addition of water helps to disperse the sample into the solvent and improve the extraction and ionization efficiency. For the analysis of the three biomarkers, the optimal ratio of methanol-water is 60%. In the negative ion mode, the addition of ammonia water provides negative ions, forming negative ion clusters in the spray solvent, which helps to further improve the signal intensity. The optimal addition concentration of ammonia water is 0.5%. Optimizing the amount of spray solvent within a certain range is the key to obtaining the best mass spectrometry results. Insufficient solvent leads to limited ionization efficiency, low ion yield and low peak intensity. Excessive solvent can cause droplet interactions and larger droplet formation, slowing down the spray speed and affecting accuracy. When the amount of spray solvent was 10 μL, the droplet size and sample diffusion rate were most appropriate, and the peak intensity was the highest.

[0062] (6) Optimization of the distance from the ion source to the discontinuous atmospheric pressure injection interface and the spray voltage

[0063] The distance from the ion source to the discontinuous atmospheric pressure injection interface is an important factor affecting the intensity of the mass spectrometry signal. When the distance is too close, the silica gel coating and impurities may enter and block the ion transport tube. Close contact increases the possibility of discharge phenomena. A distance exceeding 5mm will reduce the generation of spray, hinder the movement of droplets, and cause the signal intensity to weaken. Therefore, 5mm is the optimal distance. In addition, the ionization efficiency of the sample molecules is affected by the spray voltage. Adjusting the spray voltage can optimize ion transmission and increase signal intensity. When the spray voltage is higher than 4250V, too many ion fragments are generated, resulting in a weak signal; when the spray is lower than 4250V, the ionization efficiency is low and the signal is weak. Therefore, the final spray voltage selected is 4250V.

[0064] In addition to the single-factor main variable evaluation, the response surface methodology was used to further investigate the potential interactions between them. Based on the single-factor experiments, the effects of the amount of spray solvent, the distance from the ion source to the discontinuous atmospheric pressure injection interface, and the mass spectrometer voltage on the peak intensity were investigated. A three-factor, three-level Box-Behnken design was used to evaluate the interactions between the above variables. The results were subjected to multiple regression analysis using Design-Expert v8.0.6.1 version software. Regression analysis was performed using analysis of variance of the fitted quadratic polynomial model, and the results showed that the model was significant. Due to lack of fit, the p-value of the model was 0.1314, which accurately described the relationship between the three variables and the changes in the data. R 2 The value is 0.9201, indicating that the fitted model can explain more than 92% of the variance. The fitted model has significant linear coefficients (X2), quadratic coefficients (X1 2 , X2 2 and X3 2 ) and cross-product coefficients (X1X2 and X2X3). The results show that the amount of spray solvent, the distance between the ion source and the discontinuous atmospheric pressure injection interface, and the relationship between the mass spectrometer voltage and the peak intensity are all significantly correlated. The response surface diagrams of different variable combinations are shown in Figure 2. Figure 3 As shown, it directly reflects the interaction of the three factors on the peak intensity. Finally, the optimal conditions were determined: mass spectrometer voltage was 4250V, the distance from the ion source to the entrance was 5mm, and the amount of spray solvent was 7μL.

[0065] (7) Selection of ionization mode

[0066] In the mass spectrometry analysis, the ionization modes were compared. The results showed that the mass spectrometry signal was stronger when the negative ion mode was selected, so the negative ion mode was selected for analysis. The mass spectrometry analysis parameters were: the spray voltage was set to 4250V; the collision gas was air; the collision time was 500ms; the average number was 2 times; the continuous number was 3; the injection time was 40ms; and the mass-to-charge ratio of the injection low-end mass cutoff was m / z 50. The secondary mass spectra of the three biomarkers and their internal standards are shown in Figure 2. Figure 2 As shown. Wherein, a, c, e represent γ-aminobutyric acid, 2-hydroxyglutaric acid and N-acetyl-L-aspartic acid respectively; b, d, f represent γ-aminobutyric acid-d6, [ 2 H4]-2-Hydroxyglutarate disodium salt and N-acetyl-L-aspartate-2,3,3-d3

[0067] (8) Methodological investigation results

[0068] The performance of the method was evaluated in terms of limit of detection (LOD), limit of quantification (LOQ), linear relationship, reproducibility, recovery, matrix effect and sensitivity. The limit of detection (LOD) and limit of quantification (LOQ) of the method were estimated by 3 times signal-to-noise ratio and 10 times signal-to-noise ratio. The limit of detection (LOD) of the three biomarkers was 1μg / L, and the limit of quantification (LOQ) was 2μg / L. The calibration curve of each analyte was drawn with the concentration intensity ratio of the target analyte and the internal standard as the ordinate and the concentration of the target analyte as the abscissa. The analytes had good linearity within their respective linear ranges, and the correlation coefficients (r) of all analytes were greater than 0.99. In the spike recovery experiment, the recoveries of three spike levels, low, medium and high, were determined. The average spike recoveries of the three biomarkers were 85.9% to 107.2%, and the relative standard deviations (RSDs) were 2.4% to 8.0%. The limit of detection, limit of quantification, linear range, regression equation, correlation coefficient and recovery of the three biomarkers are shown in Table 3.

[0069] Table 3 Detection limit, quantification limit, linear range, regression equation, correlation coefficient and recovery rate of three biomarkers

[0070]

[0071] The matrix effect of the method using the utility model device was investigated. The slope ratio of the matrix matching calibration curve and the standard solution calibration curve under the same concentration range was within ±30%, indicating that the matrix interference was within an acceptable range and did not affect the quantitative analysis. In addition, the three biomarkers were detected using the traditional paper spray small portable mass spectrometry method. The results showed that the paper base was used as the separation substrate, and there was a serious matrix inhibition effect (matrix effect: -32.9% to -52.2%), which was not conducive to quantitative analysis; and the slope of the calibration curve regression equation was smaller than that of the method, indicating that the traditional paper spray ionization small portable mass spectrometry method had a low sensitivity. Therefore, the method using the utility model device has a higher sensitivity than the traditional paper spray small portable mass spectrometry method.

[0072] The embodiments described above are merely descriptions of preferred implementation modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device, characterized in that: The invention comprises a small portable mass spectrometer (2) and an aluminum-based silica gel thin layer chromatography plate (1) connected by an alligator clip (4), wherein a sample (3) is placed on the aluminum-based silica gel thin layer chromatography plate (1).

2. The two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device according to claim 1, characterized in that: The device also includes a chromatography cylinder, which includes a chromatography fluid and a filter paper sheet.

3. The two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device according to claim 1, characterized in that: The aluminum-based silica gel thin layer chromatography plate (1) is an equilateral triangle.

4. The two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device according to claim 1, characterized in that: The alligator clip (4) is made of copper. The aluminum-based silica gel thin layer chromatography plate (1) is fixed to the front end of the discontinuous atmospheric pressure injection interface of the small portable mass spectrometer (2) by the alligator clip (4). The rear end of the alligator clip (4) is connected to the small portable mass spectrometer (2) via a wire.

5. The two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device according to claim 1, characterized in that: The small portable mass spectrometer (2) is a Miniβ small portable mass spectrometer.

6. The two-dimensional thin layer chromatography-small portable mass spectrometry separation and detection device according to claim 1, characterized in that: The device also includes a glass spotting capillary, a dye solution, an electric hair dryer and a spray solvent.

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