Split type centrifugal tube for collecting trace cells of body fluid

Abstract

A split type centrifuge tube for collecting trace cells of body fluid relates to the field of detection supplies, and comprises a tube body, a sealing cover, a collecting tray and a fixed clamping plate, the tube body is composed of a cylindrical part and a conical part which are sequentially connected from top to bottom, and the cylindrical part and the conical part are detachably connected through a connecting assembly; the collecting tray is arranged in the conical part and is attached to the conical part; the fixed clamping plate is arranged on the bottom face of the cylindrical part, the collecting tray is clamped between the fixed clamping plate and the inner wall of the conical part along with connection of the cylindrical part and the conical part, the extending direction of the fixed clamping plate is the same as the inclined direction of the conical surface of the conical part, and the outer side face is matched with the inner wall of the conical surface of the conical part. By means of the centrifugal tube of the split structure, the tube body can be conveniently detached after centrifugation, the collecting tray is directly taken out from the conical part, waste and loss of cells are avoided, meanwhile, the collecting tray is clamped and fixed in the conical part through the fixing clamping plate, and it is avoided that the collecting tray breaks away from the bottom in the centrifugal process, and consequently cell collecting fails; and the success rate of cell collection is improved.

Description

Technical Field

[0001] The utility model belongs to the field of detection supplies, in particular to a split centrifuge tube for collecting micro-cells in body fluids. Background Art

[0002] Cytological testing has always been an important method for clinical pathological testing. In recent years, with the development and application of immunohistochemistry and molecular pathology, tumor diagnosis and treatment have more objective detection indicators. Due to various reasons, some patients cannot obtain tumor tissue through surgery, and body fluid cells are made into cell wax blocks instead of tumor tissue specimens for relevant pathological tests, which provide guidance for the diagnosis of tumors and the selection of clinical treatment drugs. They are chosen by more and more patients with advanced tumors or elderly patients; tumor exfoliated cells, puncture washing cells, lavage fluid, etc. After the cells are separated from the body environment, the internal structure of the cells will change. Therefore, the fixation and storage of exfoliated cells become very important. The best way to preserve cells is to make cell wax blocks, which can have the same preservation effect as tumor tissue wax blocks. Body fluid cells are centrifuged, transferred, and finally wrapped with cotton paper for dehydration and burial. In the whole process, many scattered cells will be wasted in the process of transferring from test tubes to cotton paper, which needs to be improved. Utility Model Content

[0003] The utility model aims to overcome the shortcomings of the prior art and provide a split centrifuge tube for collecting micro-cells in body fluid.

[0004] The utility model adopts the following technical solutions:

[0005] A split centrifuge tube for collecting trace cells in body fluids comprises a tube body, a sealing cover, a collection tray and a fixed clamp, the tube body consists of a cylindrical portion and a conical portion connected in sequence from top to bottom, the cylindrical portion and the conical portion are detachably connected via a connecting assembly, the tube body is recessed downward from the top surface to form a receiving cavity for receiving liquid, the diameter of the receiving cavity in the conical portion decreases as the diameter of the conical portion decreases; the sealing cover is arranged on the tube body to seal the receiving cavity; the collection tray is arranged in the conical portion and fits with the conical portion; the fixed clamp is arranged on the bottom surface of the cylindrical portion, and the collection tray is clamped between the fixed clamp and the inner wall of the conical portion as the cylindrical portion and the conical portion are connected, the extension direction of the fixed clamp is the same as the inclination direction of the conical surface of the conical portion, and the outer side surface is adapted to the inner wall of the conical surface of the conical portion.

[0006] Preferably, the connecting assembly includes an extension section, an internal thread and an external thread, the extension section is extended upward from the top surface of the conical portion and can be sleeved on the outside of the cylindrical portion; the internal thread is formed on the inner side of the extension section; the external thread is arranged on the bottom side of the cylindrical portion to match the internal thread.

[0007] Preferably, the conical portion is in the shape of a truncated cone, and its diameter gradually increases from bottom to top, the diameter of the top of the conical portion is larger than the diameter of the cylindrical portion, the extension section is arranged on the top surface of the conical portion and fits with the side surface of the cylindrical portion, and the collection tray is a truncated cone-shaped tissue paper tray that can fit with the inner bottom surface and inner side surface of the conical portion.

[0008] Preferably, a rubber pad in contact with the bottom surface of the collecting tray is provided at the bottom of the inner side surface of the conical portion, and friction patterns for increasing friction are provided on the surface of the rubber pad.

[0009] Preferably, an annular protrusion is formed on the inner side surface of the conical portion, which contacts the fixing clamping plate to clamp the collecting tray therebetween.

[0010] Preferably, the height of the collecting tray is lower than the height of the conical portion and higher than the height of the lowest point of the fixing clamp, and the setting height of the annular protrusion in the conical portion is greater than the lowest point of the fixing clamp and less than the highest point of the conical portion.

[0011] Preferably, a plurality of fixing clamps are provided and evenly distributed at the bottom of the columnar portion to evenly clamp the collecting tray.

[0012] Preferably, the columnar portion, the conical portion and the sealing cover are made of polyethylene material, and the fixing clamp extends from the bottom of the columnar portion and is made of the same polyethylene material as the columnar portion.

[0013] It can be seen from the above description of the utility model that, compared with the prior art, the utility model has the following beneficial effects: the centrifuge tube with a split structure is convenient for disassembling the tube body after centrifugation to directly take out the collection tray from the tapered portion, thereby avoiding waste and loss of cells; at the same time, the collection tray is clamped and fixed in the tapered portion by a fixing clamp, thereby avoiding the collection tray from being separated from the bottom during the centrifugation process, resulting in failure in collecting cells, thereby improving the success rate of cell collection;

[0014] The cylindrical part and the conical part are detachably connected through threaded connection, and the sealing of the connection is ensured;

[0015] The material of the collection tray is a cotton paper tray made of non-woven fabric, which is convenient for squeezing out excess water from the cells after collecting the cells;

[0016] The rubber pad provided on the inner bottom surface of the conical portion can better fit the collecting tray to the conical portion, further improving the stability of the collecting tray when installed in the conical portion;

[0017] The annular protrusion can make the collecting tray fit more closely with the fixing clamping plate, thereby improving the clamping effect of the fixing clamping plate;

[0018] The polyethylene fixing splint has a certain elasticity and can deform a certain amount, reducing the risk of damage. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the structure of the utility model;

[0020] Figure 2 It is an explosion diagram of the utility model;

[0021] Figure 3 It is a cross-sectional view of the utility model;

[0022] Figure 4 for Figure 3 A partial enlarged view of the middle A;

[0023] Figure 5 It is a structural schematic diagram of the tapered portion of the utility model;

[0024] In the figure: 1-tube body; 11-cylindrical part; 12-conical part; 121-rubber pad; 122-annular protrusion; 13-connecting assembly; 131-extension section; 132-internal thread; 133-external thread; 14-accommodating chamber; 2-sealing cover; 3-collecting tray; 4-fixing splint. DETAILED DESCRIPTION

[0025] The present invention is further described below through specific implementation methods.

[0026] Reference Figures 1 to 5 As shown, a split centrifuge tube for collecting trace cells in body fluids includes a tube body 1, a sealing cover 2, a collection tray 3, and a fixing clamp 4.

[0027] The tube body 1 is composed of a cylindrical portion 11 and a conical portion 12 connected in sequence from top to bottom. The cylindrical portion 11 and the conical portion 12 are detachably connected through a connecting assembly 13. The tube body 1 is recessed downward from the top surface to form a receiving cavity 14 for containing liquid. The conical portion 12 is truncated cone-shaped, and the diameter gradually increases from bottom to top. The diameter of the top of the conical portion 12 is larger than the diameter of the cylindrical portion 11, and the diameter of the receiving cavity 14 located in the conical portion 12 decreases as the diameter of the conical portion 12 decreases. The centrifuge tube with a split structure makes it easy to disassemble the tube body 1 after centrifugation to directly remove the cell tissue from the conical portion 12, avoiding waste and loss of cells during the transfer process.

[0028] The connecting component 13 includes an extension section 131, an internal thread 132 and an external thread 133. The extension section 131 is extended upward from the top surface of the conical portion 12. Since the diameter of the top of the conical portion 12 is larger than the diameter of the cylindrical portion 11, it can be sleeved on the outside of the cylindrical portion 11 and fit with the side of the cylindrical portion 11; the internal thread 132 is formed on the inner side of the extension section 131; the external thread 133 is arranged on the bottom side of the cylindrical portion 11 and is compatible with the internal thread 132. The cylindrical portion 11 and the conical portion 12 are detachably connected through threaded connection, and the sealing of the connection is guaranteed.

[0029] The sealing cover 2 is disposed on the tube body 1 to seal the accommodating cavity 14 . The cylindrical portion 11 , the conical portion 12 and the sealing cover 2 are made of polyethylene.

[0030] The collecting tray 3 is a truncated cone-shaped tissue paper tray that can fit with the inner bottom surface and inner side surface of the conical portion 12. Specifically, a rubber pad 121 in contact with the bottom surface of the collecting tray 3 is provided at the bottom of the inner side surface of the conical portion 12, and a friction pattern for increasing friction is provided on the surface of the rubber pad 121, thereby increasing the friction between the collecting tray 3 and the conical portion 12 and further increasing the stability of the collecting tray 3 when installed in the conical portion 12.

[0031] There are three fixed clamps 4, which are evenly distributed on the bottom surface of the cylindrical part 11 along the circumferential direction. With the connection between the cylindrical part 11 and the conical part 12, the collection tray 3 is clamped between the fixed clamps 4 and the inner wall of the conical part 12. The extension direction of the fixed clamps 4 is the same as the inclination direction of the conical surface of the conical part 12, and the outer side surface is adapted to the inner wall of the conical surface of the conical part 12. The fixed clamps 4 clamp the collection tray 3 in the conical part 12 to prevent the collection tray 3 from detaching from the bottom during the centrifugation process, resulting in failure to collect cells, thereby improving the success rate of cell collection. Specifically, an annular protrusion 122 is formed on the inner side surface of the conical part 12, which contacts the fixed clamps 4 to clamp the collection tray 3 between the two, further improving the clamping effect of the fixed clamps 4. Furthermore, the height of the collection tray 3 is lower than the height of the conical part 12 and higher than the height of the lowest point of the fixed clamps 4. The height of the annular protrusion 122 in the conical part 12 is greater than the lowest point of the fixed clamps 4 and less than the highest point of the conical part 12. The fixing clamp plate 4 extends from the bottom of the columnar portion 11 and is made of the same polyethylene material as the columnar portion 11 . The fixing clamp plate 4 made of polyethylene has a certain elasticity and can undergo a certain amount of deformation, thereby reducing the risk of damage.

[0032] Before centrifugation, the hand collection tray 3 for collecting cell tissue is first placed in the conical part 12 to fit it, and then the columnar part 11 and the conical part 12 are fixed by the connecting assembly 13. The fixed clamp plate 4 gradually approaches the inner wall of the conical part 12 as the columnar part 11 approaches the conical part 12. When the fixation is completed, the collection tray 3 is fixed between the fixed clamp plate 4 and the annular protrusion 122 to prevent the centrifugal displacement of the tray. After centrifugation, the supernatant is removed, the columnar part 11 is separated from the conical part 12, and the collection tray 3 is taken out. Since the collection tray 3 is cotton paper, the collection tray 3 is folded in half around to wrap the cells, and the subsequent dehydration and wax making steps are carried out. By designing the centrifuge tube in a split type, it is easier to take out the collection tray 3 for collecting cell tissue, avoiding the cell loss caused by the transfer process and making it easier to carry out the subsequent dehydration and wax making steps. The fixed clamp plate 4 cooperates with the annular protrusion 122 to fix the collection tray 3 in the centrifuge tube, avoiding the displacement of the collection tray 3 during the centrifugation process to cause the device to fail, thereby improving the success rate.

[0033] The above description is only a preferred embodiment of the present utility model, and therefore cannot be used to limit the scope of implementation of the present utility model. That is, equivalent changes and modifications made according to the scope of the patent application of the present utility model and the contents of the specification should still fall within the scope covered by the patent of the present utility model.

Claims

1. A split centrifuge tube for collecting micro-cells in body fluids, characterized in that: It includes a tube body, a sealing cover, a collecting tray and a fixed clamp. The tube body consists of a cylindrical part and a conical part which are connected in sequence from top to bottom. The cylindrical part and the conical part are detachably connected by a connecting assembly. The tube body is recessed downward from the top surface to form a accommodating cavity for accommodating liquid. The diameter of the accommodating cavity in the conical part decreases as the diameter of the conical part decreases. The sealing cover is arranged on the tube body to seal the accommodating cavity. The collecting tray is arranged in the conical part and fits with the conical part. The fixed clamp is arranged on the bottom surface of the cylindrical part. As the cylindrical part is connected to the conical part, the collecting tray is clamped between the fixed clamp and the inner wall of the conical part. The extending direction of the fixed clamp is the same as the inclination direction of the conical surface of the conical part, and the outer side surface is adapted to the inner wall of the conical surface of the conical part.

2. A split centrifuge tube for collecting micro-cells in body fluids according to claim 1, characterized in that: The connecting assembly includes an extension section, an internal thread and an external thread. The extension section is extended upward from the top surface of the conical portion and can be sleeved on the outside of the cylindrical portion. The internal thread is formed on the inside of the extension section. The external thread is arranged on the bottom side of the cylindrical portion to match the internal thread.

3. A split centrifuge tube for collecting micro-cells in body fluid according to claim 2, characterized in that: The conical portion is in the shape of a truncated cone, and its diameter gradually increases from bottom to top. The diameter of the top of the conical portion is larger than the diameter of the cylindrical portion. The extension section is arranged on the top surface of the conical portion and fits with the side surface of the cylindrical portion. The collection tray is a truncated cone-shaped tissue paper tray that can fit with the inner bottom surface and inner side surface of the conical portion.

4. A split centrifuge tube for collecting micro-cells in body fluid according to claim 3, characterized in that: A rubber pad in contact with the bottom surface of the collecting tray is arranged at the bottom of the inner side surface of the conical portion, and friction lines that improve friction are arranged on the surface of the rubber pad.

5. The split centrifuge tube for collecting micro-cells in body fluid according to claim 1, characterized in that: An annular protrusion is formed on the inner side surface of the conical portion, which contacts the fixing clamping plate to clamp the collecting tray therebetween.

6. A split centrifuge tube for collecting micro-cells in body fluid according to claim 5, characterized in that: The height of the collecting tray is lower than the height of the conical portion and higher than the height of the lowest point of the fixing clamp, and the height of the annular protrusion in the conical portion is higher than the lowest point of the fixing clamp and lower than the highest point of the conical portion.

7. The split centrifuge tube for collecting micro-cells in body fluid according to claim 1, characterized in that: A plurality of fixing clamps are provided and evenly distributed at the bottom of the columnar portion to evenly clamp the collecting tray.

8. The split centrifuge tube for collecting micro-cells in body fluid according to claim 1, characterized in that: The columnar portion, the conical portion and the sealing cover are made of polyethylene material. The fixing clamp extends from the bottom of the columnar portion and is made of the same polyethylene material as the columnar portion.

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