Red blood cell lysis kit for flow cytometry
By designing a flow cytometry erythrocyte lysis kit with sliders and handhelds, the problem of multiple erythrocyte lysis and kit washing in the prior art is solved, and a more efficient and accurate cell lysis process is achieved.
Patent Information
- Application Number
- CN202421060675.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-15
- Publication Date
- 2025-04-22
- Estimated Expiration
- 2034-05-15
AI Technical Summary
Existing cell lysis experiments require multiple erythrocyte lysis, and the kit needs to be washed after the first lysis, which affects the accuracy of the experimental data results.
A erythrocyte lysis kit for flow cytometry is designed, including the kit shell, lysis chamber, reagent chamber, slider, handheld, nest, slider, vertical groove, water tank, wastewater chamber and water filter chamber. Through the movement of the slider and handheld, the uniform mixing and continuous operation process of the reagent is achieved, reducing the number of washes of the kit.
It improves the efficiency and accuracy of erythrocyte lysis, reduces experimental steps and time, and reduces the number of washes of the kit, thereby improving the accuracy of experimental results.
Smart Images
Figure CN222784379U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field related to flow cytometry, and specifically relates to a red blood cell lysis kit for flow cytometry. Background Art
[0002] Before performing flow cytometry, it is usually necessary to remove red blood cells from the sample because red blood cells will interfere with the detection of other types of cells by the flow cytometer. The red blood cell lysis method uses a lysis buffer to lyse red blood cells, which does not damage nucleated cells and can fully remove red blood cells. It is a gentle, simple and easy method to remove red blood cells. The red blood cell lysis method can be used for the separation and purification of tissue cells dispersed by enzyme digestion, the separation and purification of lymphocytes, and the removal of red blood cells in experiments such as tissue cell protein and nucleic acid extraction. The tissue cells obtained by lysis with red blood cell lysis buffer do not contain red blood cells and can be further used for primary culture, cell fusion, flow cytometric analysis, separation and extraction of nucleic acids and proteins, etc.
[0003] However, the existing experimental operation of cell lysis has a great impact on the test results. It usually requires multiple lysis of red blood cells, and the reagent kit needs to be washed after the first lysis, which will have a certain degree of impact on the accuracy of the experimental data results. Utility Model Content
[0004] The purpose of the utility model is to provide a red blood cell lysis kit for flow cytometry, so as to solve the problem that the existing cell lysis experimental operation proposed in the above background technology has a great influence on the detection results, usually requires multiple lysis of red blood cells, and the kit needs to be washed after the first lysis, which will affect the accuracy of the experimental data results to a certain extent.
[0005] To achieve the above-mentioned object, the utility model provides the following technical solutions: a red blood cell lysis kit for flow cytometry, comprising a kit housing;
[0006] A lysis chamber is arranged at an inner position of the reagent kit housing, a reagent compartment is arranged in an array at a middle position inside the lysis chamber, and a lysis solution is arranged at an inner position of the reagent compartment;
[0007] A baffle is arranged at the middle position inside the reagent kit housing, a water tank is arranged at the right position of the reagent kit housing, and a sliding part is arranged at the upper position of the lysis chamber.
[0008] Preferably, a lysis chamber is provided at the bottom of the sliding member, a hand piece is provided at the right side of the lysis chamber, a nesting piece is provided at the top of the lysis chamber, the lysis chamber is slidably connected to the sliding member, and an internal array of pores with a diameter of one micron is opened in the lysis chamber.
[0009] Preferably, a slide groove is provided at a position above the lysis chamber, the slide groove corresponds to the sliding member, and a vertical groove is provided at a position below the slide groove on the right side of the reagent kit housing.
[0010] Preferably, a liquid filling port is provided at the middle position of the top of the water tank, the handpiece is fixedly connected to the lysis chamber, four of the water tank, reagent chamber and vertical slots are arranged in an array respectively, and a partition is provided at the middle position of the water tank.
[0011] Preferably, a wastewater bin is provided at the bottom of the reagent kit housing, four water filtration bins are arranged in an array inside the lysis chamber, and a wastewater outlet is provided at the left side of the wastewater bin.
[0012] Preferably, a bottom plate is provided at the bottom of the reagent compartment, an insert plate is provided at the middle position inside the bottom plate, a slot is provided at the left side of the bottom plate, and the insert plate is slidably connected to the bottom plate.
[0013] Preferably, a liquid outlet is provided at the bottom of the water tank, the liquid outlet is connected to the reagent compartment, and the reagent box housing is made of plastic.
[0014] Compared with the prior art, the utility model provides a red blood cell lysis kit for flow cytometry, which has the following beneficial effects:
[0015] 1. Through the arrangement of the sliding piece, the handpiece, the lysis chamber, the nesting piece, the slide groove, the vertical groove, the liquid filling port and the water tank, by moving the sliding piece to the specified position, it is possible to ensure that the handpiece and the lysis chamber are accurately positioned during the lysis operation, thereby effectively controlling the precision and accuracy of the operation. After the lysis chamber is immersed in the bottom reagent chamber, the handpiece is pushed downward to effectively mix the reagents, ensuring that the reagents can evenly cover the cell surface during the lysis process, thereby improving the lysis efficiency. By sliding the handpiece upward and moving the sliding piece to enter the next reagent chamber, a continuous operation process can be achieved, the operation efficiency is improved, time is saved, and the entire experiment is accelerated.
[0016] 2. Through the setting of wastewater bin, filter bin, wastewater outlet, bottom plate, plug plate and slot, the filtered reagent can remove possible impurities or waste, maintain the purity of the reagent, reduce interference with the next step of operation, thereby improving the accuracy of the experimental results. Staying above the filter bin to filter the reagent makes the operation process more flexible and can be adjusted according to actual needs to meet the needs of different experimental conditions. When the wastewater bin at the bottom is full, the internal wastewater is discharged through the wastewater outlet. A slot is set on the left side of the bottom plate to improve the sealing of the plug plate and the baffle, reducing the loss of reagents during use. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] Figure 1It is a structural schematic diagram of the utility model.
[0018] Figure 2 It is a schematic structural diagram of the test kit housing in the utility model.
[0019] Figure 3 It is a structural schematic diagram of the sliding member in the utility model.
[0020] Figure 4 It is a structural schematic diagram of the cross section of the test kit shell in the utility model.
[0021] In the figure: 1. reagent box shell; 2. liquid filling port; 3. baffle; 4. water tank; 5. nesting part; 6. lysis chamber; 7. sliding part; 8. hand piece; 9. plug-in plate; 10. reagent chamber; 11. water filter chamber; 12. lysis chamber; 13. slot; 14. slide; 15. bottom plate; 16. waste water chamber; 17. waste water outlet; 18. partition; 19. liquid outlet; 20. vertical slot. DETAILED DESCRIPTION
[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0023] The utility model provides Figure 1-4 The red blood cell lysis kit for flow cytometry shown includes a kit housing 1;
[0024] A lysis chamber 12 is provided at an inner position of the reagent box housing 1, a reagent compartment 10 is arranged in an array at a middle position inside the lysis chamber 12, and a lysis solution is provided at an inner position of the reagent compartment 10;
[0025] A baffle 3 is arranged at the middle position inside the reagent kit housing 1 , a water tank 4 is arranged at the right position of the reagent kit housing 1 , and a sliding member 7 is arranged at the position above the lysis chamber 12 .
[0026] A cracking chamber 6 is arranged at the bottom of the sliding member 7, a hand piece 8 is arranged at the right side of the cracking chamber 6, a nesting piece 5 is arranged at the top of the cracking chamber 6, the cracking chamber 6 is slidably connected to the sliding member 7, and the internal array of the cracking chamber 6 is provided with pores with a diameter of one micron.
[0027] A slide groove 14 is provided above the lysis chamber 12 , and the slide groove 14 corresponds to the sliding member 7 . A vertical groove 20 is provided below the slide groove 14 on the right side of the reagent kit housing 1 .
[0028] A liquid filling port 2 is provided at the middle position of the top of the water tank 4 , the hand piece 8 is fixedly connected to the lysis chamber 6 , the water tank 4 , the reagent chamber 10 and the vertical slots 20 are arranged in four arrays respectively, and a partition 18 is provided at the middle position of the water tank 4 .
[0029] A wastewater bin 16 is disposed at the bottom of the reagent kit housing 1 , four water filtration bins 11 are arranged in an array inside the lysis chamber 12 , and a wastewater port 17 is disposed at the left side of the wastewater bin 16 .
[0030] A bottom plate 15 is provided at the bottom of the reagent chamber 10 , an inserting plate 9 is provided at the middle position inside the bottom plate 15 , a slot 13 is provided at the left side of the bottom plate 15 , and the inserting plate 9 is slidably connected to the bottom plate 15 .
[0031] A liquid outlet 19 is provided at the bottom of the water tank 4 , and the liquid outlet 19 is connected to the reagent compartment 10 . The reagent box housing 1 is made of plastic.
[0032] In this embodiment, the specific implementation steps of a red blood cell lysis kit for flow cytometry are as follows: prepare the lysis solution, add 10 times the pre-diluted lysis solution into the first two water tanks 4 from right to left, dilute it to 1 times with deionized water, add washing solution and cell staining buffer into the last two water tanks 4, so that the sliding part 7 is immersed in the reagent compartment 10 on the front side of the water tank 4 in turn, and stops above the water filter compartment 11 to filter the reagent before entering the next reagent compartment 10. When the cell lysis is completed, pull out the plug plate 9 to allow the excess reagent to flow into the bottom wastewater compartment 16 to wait for the next cell lysis.
[0033] like Figure 1-3 As shown, a lysis chamber 6 is provided at the bottom of the sliding member 7, a hand piece 8 is provided at the right side of the lysis chamber 6, a nesting piece 5 is provided at the top of the lysis chamber 6, the lysis chamber 6 is slidably connected to the sliding member 7, an internal array of pores with a diameter of one micron is provided in the lysis chamber 6, a slide groove 14 is provided above the lysis chamber 12, the slide groove 14 corresponds to the sliding member 7, a vertical groove 20 is provided below the slide groove 14 on the right side of the reagent box shell 1, a liquid filling port 2 is provided at the middle position of the top of the water tank 4, the hand piece 8 is fixedly connected to the lysis chamber 6, four water tanks 4, reagent chambers 10 and vertical grooves 20 are respectively provided in arrays, and a partition 18 is provided in the middle of the water tank 4.
[0034] Preferably, the sliding member 7 is moved so that the sliding member 7 reaches the specified position, and then the handpiece 8 is pushed downward so that the lysis chamber 6 is immersed in the bottom reagent chamber 10 to lyse the cells. After completing this lysis step, the handpiece 8 is slid upward, and the sliding member 7 is moved to enter the next reagent chamber. The internal array of the lysis chamber 6 has pores with a diameter of one micron to separate the lysed red blood cells. A vertical groove 20 is provided below the slide groove 14 on the right side of the reagent box housing 1 to position the handpiece 8 and increase the speed of the sliding member 7. A liquid filling port 2 is provided in the middle position at the top of the water tank 4 to increase the reagent content.
[0035] like Figure 4 As shown, a wastewater bin 16 is provided at the bottom of the reagent box shell 1, four water filtration bins 11 are arranged in an array inside the lysis chamber 12, a wastewater port 17 is provided at the left side of the wastewater bin 16, a bottom plate 15 is provided at the bottom of the reagent bin 10, an insert plate 9 is provided at the middle position inside the bottom plate 15, a slot 13 is provided at the left side of the bottom plate 15, and the insert plate 9 is slidably connected to the bottom plate 15.
[0036] Preferably, during the lysis process, the reagent is filtered out by staying above the filter bin 11 before entering the next reagent bin 10. After the cell lysis is completed, the plug plate 9 is pulled outward to allow excess reagent to flow into the bottom waste water bin 16. When the bottom waste water bin 16 is full, the internal waste water is discharged through the waste water port 17. A slot 13 is provided at the left side of the bottom plate 15 to improve the sealing performance of the plug plate 9 and the baffle 3 and reduce the loss of reagents during use.
[0037] like Figure 1-4 As shown, a liquid outlet 19 is provided at the bottom of the water tank 4, and the liquid outlet 19 is connected to the reagent compartment 10. The reagent box housing 1 is made of plastic.
[0038] Optionally, a liquid outlet 19 is opened at the bottom of the water tank 4, and the liquid outlet 19 is connected to the reagent chamber 10. Liquid can be directly added to the reagent chamber 10 through the water tank 4 to increase the liquid adding speed. The reagent box shell 1 is made of plastic, and plastic has good resistance to most chemical substances, so that the reagent box shell 1 can safely store and handle chemical reagents without corrosion or damage.
[0039] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
Claims
1. A red blood cell lysis kit for flow cytometry, comprising a kit housing (1); A lysis chamber (12) is arranged at an internal position of the reagent kit housing (1), a reagent compartment (10) is arranged in an array at a middle position inside the lysis chamber (12), and a lysis solution is arranged at an internal position of the reagent compartment (10); Features: A baffle (3) is arranged at the middle position inside the reagent kit housing (1), a water tank (4) is arranged at the right position of the reagent kit housing (1), and a sliding member (7) is arranged at the upper position of the lysis chamber (12).
2. The red blood cell lysis kit for flow cytometry according to claim 1, characterized in that: A lysis chamber (6) is arranged at the bottom of the sliding member (7), a hand piece (8) is arranged at the right side of the lysis chamber (6), a nesting piece (5) is arranged at the top of the lysis chamber (6), the lysis chamber (6) is slidably connected to the sliding member (7), and an internal array of pores with a diameter of one micron is opened in the lysis chamber (6).
3. A red blood cell lysis kit for flow cytometry according to claim 2, characterized in that: A slide groove (14) is provided above the lysis chamber (12), and the slide groove (14) corresponds to the sliding member (7). A vertical groove (20) is provided below the slide groove (14) on the right side of the reagent kit housing (1).
4. A red blood cell lysis kit for flow cytometry according to claim 3, characterized in that: A liquid filling port (2) is provided at the middle position of the top of the water tank (4), the hand piece (8) is fixedly connected to the lysis chamber (6), the water tank (4), the reagent chamber (10) and the vertical slot (20) are respectively arranged in four arrays, and a partition (18) is provided at the middle position of the water tank (4).
5. The red blood cell lysis kit for flow cytometry according to claim 1, characterized in that: A wastewater bin (16) is arranged at the bottom of the reagent box housing (1), four water filtration bins (11) are arranged in an array inside the lysis chamber (12), and a wastewater outlet (17) is arranged at the left side of the wastewater bin (16).
6. The red blood cell lysis kit for flow cytometry according to claim 5, characterized in that: A bottom plate (15) is arranged at the bottom of the reagent chamber (10), an insert plate (9) is arranged at the middle position inside the bottom plate (15), a slot (13) is arranged at the left side of the bottom plate (15), and the insert plate (9) is slidably connected to the bottom plate (15).
7. The red blood cell lysis kit for flow cytometry according to claim 1, characterized in that: A liquid outlet (19) is provided at the bottom of the water tank (4), and the liquid outlet (19) is connected to the reagent compartment (10). The reagent box housing (1) is made of plastic.