ELISA filter paper

Abstract

The utility model relates to the technical field of filter paper, in particular to ELISA (Enzyme-Linked Immunosorbent Assay) filter paper. In order to improve liquid emptying efficiency and reduce noise when an elisa plate is flapped, a first flexible barrier strip is fixedly connected to a filter paper body to form a closed structure, and the closed structure is used for limiting the planar degree of freedom of an object on the filter paper body in the closed structure. When the absorbent paper is placed on the ELISA filter paper and the elisa plate is inverted on the absorbent paper, the elisa plate is flapped at the position, corresponding to the first flexible barrier strip, of the absorbent paper, and when residual liquid is removed, noise can be reduced, so that flapping can be carried out with high frequency and high strength, the cleaning effect is improved, on one hand, whether the filter paper body is infiltrated or not is observed, and on the other hand, whether the filter paper body is infiltrated or not is judged. The opportunity of replacing the absorbent paper can be known; when the ELISA plate is matched with a container for use, ELISA filter paper is placed on the container, at the moment, the flapping height of the ELISA plate is increased, and generated airflow is far away from the table top, so that the influence on light and small objects on the table top is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of filter paper, in particular to an ELISA filter paper. Background Art

[0002] ELISA experiments can be used to qualitatively or quantitatively detect specific antigens or antibodies in samples, and are widely used in biomedicine, food safety, environmental monitoring and other fields. The basic principle of ELISA experiments is: fix the antigen or antibody on a solid phase carrier, then add the sample to be tested, so that the antigen or antibody in the sample binds to the antigen or antibody on the solid phase carrier. Next, add the enzyme-labeled antibody or antigen to react with the antigen or antibody bound to the solid phase carrier. Finally, add the enzyme substrate to make the enzyme catalyze the substrate to react and produce a color change. The content of the antigen or antibody in the sample is judged based on the degree of color change.

[0003] In this process, an ELISA plate microplate is used as a solid phase carrier. After each short incubation, the cleaning process is required: the ELISA plate is cleaned with a cleaning solution, then inverted on absorbent paper, and the plate is tapped to remove residual liquid. Due to the high frequency of tapping, and in order to improve the cleaning effect, the ELISA plate needs to be tapped harder, which generates noise; at the same time, when tapping harder, the airflow generated will blow away small items placed on the desktop, such as spare absorbent paper, causing interference to the experiment, so it is usually necessary to control the strength and tap the ELISA plate gently; at the same time, the absorbent paper is generally a white thin sheet structure, and it is difficult to observe the degree of water absorption, and it is inconvenient to grasp the timing of replacing the absorbent paper, which brings inconvenience to the experiment. Utility Model Content

[0004] The utility model provides an ELISA filter paper, aiming to improve the convenience of the enzyme labeling plate cleaning link.

[0005] The above purpose is achieved through the following technical solutions:

[0006] An ELISA filter paper comprises a filter paper body, on which a first flexible baffle is fixedly connected. The first flexible baffle forms a closed structure on the filter paper body for limiting the plane freedom of an object on the filter paper body within the closed structure.

[0007] Preferably, the ELISA filter paper is used in conjunction with absorbent paper, and the absorbent paper is placed on the upper end of the first flexible baffle.

[0008] Preferably, the ELISA filter paper is used in conjunction with a container, and the upper edge of the container can support the first flexible baffle.

[0009] Preferably, the inner periphery or the inner and outer periphery edges of the closed structure are rectangular.

[0010] The beneficial effects of the ELISA filter paper of the utility model are:

[0011] To solve the problem mentioned in the background technology, absorbent paper is placed on the ELISA filter paper, and when the ELISA plate is inverted on the absorbent paper, the ELISA plate is tapped on the absorbent paper at the position corresponding to the first flexible baffle, and the noise can be eliminated when removing the residual liquid, so that the tapping can be performed at a higher frequency and a higher force to improve the cleaning effect. On the other hand, by observing whether the filter paper body is soaked, the timing of replacing the absorbent paper can be known; when used with a container, the ELISA filter paper is placed on the container, and the height of the ELISA plate when tapping is raised, and the airflow generated is away from the desktop to avoid affecting light and small objects on the desktop. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 It is a structural schematic diagram of the filter paper body and the first flexible baffle;

[0013] Figure 2 is a schematic diagram of a hole on the first flexible baffle;

[0014] Figure 3 is a schematic diagram of the holes on the filter paper body;

[0015] Figure 4 The structure diagram of the second flexible baffle Figure 1 ;

[0016] Figure 5 The structure diagram of the second flexible baffle Figure 2 ;

[0017] Figure 6 It is a schematic diagram of the structure of the container body and the limiting member;

[0018] Figure 7 It is a schematic diagram showing that the filter paper body and the first flexible baffle are plugged into the limiting member, and the filter paper body is placed on the upper end of the container body;

[0019] Figure 8 Schematic diagram of actual operation of filter paper body.

[0020] In the figure: 1. filter paper body; 2. first flexible baffle; 3. hole; 4. second flexible baffle; 5. container body; 6. limiter. DETAILED DESCRIPTION

[0021] An ELISA filter paper, reference Figure 1, including a filter paper body 1. To facilitate the beating of the ELISA plate, a first flexible baffle 2 is fixed to the upper end of the filter paper body 1 by adhesive; preferably, the first flexible baffle 2 is sterile silicone or rubber; the adhesive can be silicone adhesive, silicone adhesive or epoxy adhesive, without limitation, and other adhesives in the prior art can also be used.

[0022] The first flexible baffle 2 may be an integral structure or a structure bonded end to end, so that the first flexible baffle 2 forms a closed structure on the filter paper body 1;

[0023] During the cleaning process of the ELISA plate, the absorbent paper is laid on the upper end of the closed structure. When the ELISA plate is turned over, the ELISA plate is buckled on the absorbent paper, which can increase the force of hitting and bumping the ELISA plate, and the ELISA plate is repeatedly bumped against the first flexible baffle 2, thereby playing a role in buffering and reducing noise, and preventing the cleaning liquid from separating from the filter paper body 1; refer to Figure 8 , the one with a circular outline is absorbent paper, and the one held in hand is an inverted ELISA plate.

[0024] Among them, in order to adapt to the size of the ELISA plate so that the edge of the ELISA plate can be hit on the first flexible baffle 2, the closed structure formed by the first flexible baffle 2 is preferably a rectangular thin-walled structure, and the upper end face is a horizontal end face. At the same time, the length and width of the first flexible baffle 2 correspond to the length and width of the ELISA plate.

[0025] On the other hand, when the absorbent paper is full of cleaning liquid, the cleaning liquid will seep onto the filter paper body 1, so by observing whether the filter paper body 1 has been immersed in liquid, it can be known whether the absorbent paper needs to be replaced;

[0026] In order to facilitate observation of whether the filter paper body 1 is wet, preferably, the filter paper body 1 is colored, such as black, gray, yellow, blue or purple, so that the wetted area and other non-wetted areas have different refractions of light, resulting in color difference. It should be noted that absorbent paper is not easy to be processed into colored paper because the absorbent paper is in direct contact with the ELISA plate, which may contaminate the ELISA plate.

[0027] The filter paper body 1 is one of cellulose filter paper, glass fiber filter paper and polyamide filter paper.

[0028] In order to collect the used cleaning solution, ELISA filter paper is used in conjunction with a container, and the filter paper body 1 is placed on the upper edge of the container. At the same time, the upper edge of the container corresponds to the size of the first flexible baffle 2, so that the ELISA filter paper can be stably supported.

[0029] In order to improve the stability of ELISA filter paper, refer to Figure 6The container comprises a container body 5, and two to four stoppers 6 respectively fixed to the upper end of the container body 5, the stoppers 6 are preferably columnar, and the stoppers 6 are preferably located at the four corners of the upper end of the container body 5, and the container body 5 and the stoppers 6 can be integrally formed by injection molding; on the other hand, referring to Figure 2 and 3 The four corners of the filter paper body 1 and the four corners of the first flexible baffle 2 are provided with holes 3 for matching the limiter 6, that is, the hole 3 corresponds to the position of the limiter 6, and the hole 3 on the container body 5 is connected to the hole 3 on the first flexible baffle 2; Figure 7 When the limiter 6 passes through the hole 3, it can be inserted into the filter paper body 1 and the first flexible baffle 2 at the same time; at this time, the left and right ends, or the front and back ends of the ELISA plate can be tapped to mix the groups of samples just added to the ELISA plate.

[0030] Preferably, reference Figure 4 and 5 A second flexible baffle 4 is fixedly connected to the filter paper body 1, which divides the closed structure formed by the first flexible baffle 2 into two closed areas. The height of the first flexible baffle 2 is recorded as h1, and the height of the second flexible baffle 4 is recorded as h2, and h1≥h2.

[0031] The first flexible baffle 2 and the second flexible baffle 4 are made of the same material. In addition, when the first flexible baffle 2 is silicone, the compression amount of the silicone is stipulated as: 0.9h1≥h1-h2≥0.6h1, so that the first flexible baffle 2 contracts when being tapped by the ELISA plate, and the upper end surface of the first flexible baffle 2 easily overlaps with the second flexible baffle 4. The ELISA plate can be tapped on the second flexible baffle 4, which plays a role of tactile feedback, prompting the experimenter that the tapping force is sufficient.

[0032] Preferably, the height of the limiting member 6 is not higher than h2.

Claims

1. An ELISA filter paper, comprising a filter paper body (1), characterized in that: A first flexible baffle (2) is fixedly connected to the filter paper body (1), and the first flexible baffle (2) forms a closed structure on the filter paper body (1) for limiting the plane freedom of an object on the filter paper body (1) within the closed structure.

2. The ELISA filter paper according to claim 1, characterized in that The filter paper body (1) is one of cellulose filter paper, glass fiber filter paper and polyamide filter paper.

3. The ELISA filter paper according to claim 1, characterized in that The filter paper body (1) is colorful.

4. The ELISA filter paper according to claim 1, characterized in that The first flexible barrier strip (2) is made of silica gel or rubber.

5. The ELISA filter paper according to claim 1, characterized in that The inner periphery or the inner and outer periphery edges of the closed structure are rectangular.

6. The ELISA filter paper according to claim 5, characterized in that Used in conjunction with absorbent paper, the absorbent paper is placed on the upper end of the first flexible baffle (2).

7. The ELISA filter paper according to claim 1 or 6, characterized in that When used in conjunction with a container, the upper edge of the container can support the first flexible barrier strip (2).

8. The ELISA filter paper according to claim 7, characterized in that The container comprises a container body (5) and a stopper (6) fixedly connected to the upper end of the container body (5); holes (3) are provided on the filter paper body (1) and the first flexible stopper (2); the holes (3) enable the four corners of the filter paper body (1) and the four corners of the first flexible stopper (2) to be inserted into the stopper (6).

9. The ELISA filter paper according to claim 1, characterized in that A second flexible baffle (4) is fixedly connected to the filter paper body (1) and divides the closed structure formed by the first flexible baffle (2) into two closed areas. The height of the first flexible baffle (2) is denoted as h1, and the height of the second flexible baffle (4) is denoted as h2, where h1≥h2.

10. The ELISA filter paper according to claim 9, characterized in that 0.9h1≥h1-h2≥0.6h1.

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