Fluorescent quantitative RT-PCR (reverse transcription-polymerase chain reaction) kit with built-in cushion layer capable of automatically lifting

Abstract

The utility model belongs to the technical field of kits, and particularly relates to a fluorescent quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) kit with a built-in cushion layer capable of automatically lifting. The utility model comprises: a box body; the box cover is arranged on the top surface of the box body, and the box cover can rotate along one side of the box body; the buffer cushion layer is arranged inside the kit body, the buffer cushion layer is provided with a plurality of accommodating holes with different sizes and a probe groove, and the accommodating holes are respectively used for placing reagent tubes with different diameters; the two groups of lifting mechanisms are arranged between the buffer cushion layer and the bottom plate of the box body; one ends of the two traction ropes are connected with the two lifting mechanisms respectively, and the other ends of the two traction ropes are connected with the box cover respectively. The kit provided by the utility model is used for solving the technical problem that an operator cannot conveniently and quickly take a required reagent tube in the kit in the prior art.

Description

Technical Field

[0001] The utility model belongs to the technical field of test kits, and in particular relates to a fluorescent quantitative RT-PCR test kit with a built-in cushion layer that automatically lifts. Background Art

[0002] The fluorescent quantitative RT-PCR kit in the prior art generally includes an outer packaging box and several reagent tubes, and the reagent tubes are inserted into the foam cotton or plastic plate in the outer packaging box. Therefore, when the kit is opened for use, it is necessary to open the outer packaging box first, and then reach into the outer packaging box to extract the reagent tubes, which makes it inconvenient to take the reagent tubes.

[0003] In addition, in some genetic testing processes, the shorter the interval between adding reagents, primers and other materials, the better the test effect; otherwise, there will be a risk of affecting the accuracy of the test results. Therefore, the inability to quickly take the reagent tube is also one of the disadvantages of existing test kits. Utility Model Content

[0004] The technical problem to be solved by the utility model is to overcome the deficiencies of the prior art and provide a fluorescent quantitative RT-PCR kit with a built-in cushion layer that automatically lifts, so as to solve the technical problem that the kit in the prior art cannot allow operators to conveniently and quickly take the required reagent tubes.

[0005] The utility model solves the above technical problems with the following technical solutions: A fluorescent quantitative RT-PCR kit, comprising:

[0006] Box body;

[0007] A box cover, which is arranged on the top surface of the box body and can rotate along one side of the box body;

[0008] A buffer layer, the buffer layer is arranged inside the box body, the buffer layer is provided with a plurality of accommodating holes of different sizes and a probe groove, and the plurality of accommodating holes are respectively used to place reagent tubes of different diameters;

[0009] At least two groups of lifting mechanisms, wherein the two groups of lifting mechanisms are arranged between the buffer layer and the bottom plate of the box body;

[0010] At least two traction ropes, one end of the two traction ropes is respectively connected to the two lifting mechanisms, and the other end is respectively connected to the box cover.

[0011] The buffer layer in the utility model is used to store reagent tubes and probes, and the buffer layer is placed in the box body, and then the box cover is connected to the lifting mechanism through a traction rope, so that the box cover and the lifting mechanism are linked, and when the box cover is opened, the lifting mechanism can be driven to lift the buffer layer, so that the buffer layer protrudes out of the box body, which is convenient for operators to take corresponding reagent tubes and probes; when the box cover is closed, the lifting mechanism is reset, so that the buffer layer falls back into the box body, thereby protecting the reagent tubes.

[0012] Further: the lifting mechanism includes a hinged column arranged on the bottom plate of the box body, and a push rod hinged to the hinged column, and a slide groove is opened at a position of the bottom plate of the box body corresponding to the push rod, a slider is arranged in the slide groove, and the outer side surface of the slider is connected to the inner wall of the box body through a spring;

[0013] And the height of the sliding block is greater than the height of the hinge column;

[0014] One end of the traction rope is connected to the slider.

[0015] Further: the lifting mechanism includes two hinged columns arranged on the bottom plate of the box body, and the hinged column close to the opening of the box cover is a fixed column, and the hinged column away from the opening of the box cover is a sliding column, and the sliding column is arranged in a sliding groove of the bottom plate of the box body;

[0016] Two connecting rods are also provided between the two hinged columns, the outer ends of the two connecting rods are hinged to the hinged columns respectively, and the inner ends of the two connecting rods are hinged to each other;

[0017] A spring is also provided between the two hinged columns;

[0018] The traction rope is connected to the sliding hinge column.

[0019] The beneficial effect of adopting this step: the overall function of the above-mentioned two lifting mechanisms is to drive the slider or the sliding hinged column to contract through the traction rope, lift the top rod or fold and lift the connecting rod, so as to achieve the function of lifting the buffer layer. The only difference between the two is that within the same stroke, the lifting height of the folding connecting rod is greater, which can make the buffer layer protrude more from the box body.

[0020] Furthermore: a rope groove is provided on the buffer layer at a position corresponding to the traction rope.

[0021] The beneficial effect of adopting this step is that the rope groove is used to make room for the traction rope and to limit the traction rope to ensure that the traction rope is in the same vertical plane relative to the lifting mechanism.

[0022] Further: one side of the box cover and one side of the box body are connected by a hinge;

[0023] Alternatively, the box cover and the box body are integrally injection-molded, and a bending belt is provided between the box cover and the box body.

[0024] The beneficial effect of adopting this step is: different composition forms of the box body and the box cover are shown.

[0025] Furthermore: the box cover is connected to the other side of the box body through a buckle, a hook or a locking piece.

[0026] The beneficial effect of adopting this step is: it shows the locking structure of the box cover and the box body.

[0027] Furthermore: the material of the buffer layer is thermoplastic elastomer.

[0028] The beneficial effects of adopting this step are as follows: the thermoplastic elastomer has elasticity and softness similar to that of rubber and can maintain its elastic shape at room temperature; this property enables the cushion layer to adapt to the box body, and the size of the accommodating hole can be smaller than the size of the reagent tube to achieve the purpose of wrapping the reagent tube; in addition, the thermoplastic elastomer also has the characteristics of wear resistance, weather resistance, chemical stability and colorability.

[0029] Furthermore: a clearance gap is also provided on the top surface of the probe groove.

[0030] The beneficial effect of adopting this step is that the clearance notch can reduce the distance between the top surface and the bottom of the probe groove, making it more convenient to take the probe.

[0031] The beneficial effects of the utility model are:

[0032] 1. The buffer layer used to place the reagent tube and the probe in the present application can move up when the box cover is opened and move down when the box cover is closed under the action of the linked box cover and the lifting mechanism. When the box cover is opened, the buffer layer can be lifted to allow the reagent tube on the buffer layer to protrude from the box body. This synchronous opening and moving structure can not only save the operator's time in taking the reagent tube, but also make it easier for the operator to take the reagent tube without having to put his hand into the reagent box;

[0033] 2. This application places the probe and the reagent tube together inside the box body to prevent the probe from being lost. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0035] Figure 1A stereoscopic diagram of a fluorescent quantitative RT-PCR kit with a built-in cushion layer and automatic lifting provided by the utility model;

[0036] Figure 2 The working state of the first embodiment of the fluorescent quantitative RT-PCR kit with built-in cushion layer and automatic lifting provided by the utility model Figure 1 ;

[0037] Figure 3 The working state of the first embodiment of the fluorescent quantitative RT-PCR kit with built-in cushion layer and automatic lifting provided by the utility model Figure 2 ;

[0038] Figure 4 The working state of the second embodiment of the fluorescent quantitative RT-PCR kit with built-in cushion layer and automatic lifting provided by the utility model Figure 1 ;

[0039] Figure 5 The working state of the second embodiment of the fluorescent quantitative RT-PCR kit with built-in cushion layer and automatic lifting provided by the utility model Figure 2 ;

[0040] Figure 6 The utility model provides a structural schematic diagram of a fluorescent quantitative RT-PCR kit with a built-in cushion layer and automatic lifting.

[0041] Reference numerals:

[0042] 1-box body; 2-box cover; 3-buffer layer; 4-lifting assembly embodiment 1; 4'-lifting assembly embodiment 2; 5-traction rope; 6-reagent tube;

[0043] 31-probe slot; 41-hinged column; 42-rod; 43-slider; 44-spring; 45-sliding column; 46-connecting rod. DETAILED DESCRIPTION

[0044] The following embodiments of the technical solution of the utility model are described in detail in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the utility model, and are therefore only used as examples, and cannot be used to limit the protection scope of the utility model.

[0045] It should be noted that, unless otherwise specified, the technical terms or scientific terms used in this application should have the common meanings understood by technicians in the field to which the utility model belongs.

[0046] Example

[0047] like Figure 1 As shown, the utility model provides a fluorescent quantitative RT-PCR kit, comprising:

[0048] Box body 1;

[0049] A box cover 2, wherein the box cover 2 is arranged on the top surface of the box body 1, and the box cover 2 can rotate along one side of the box body 1;

[0050] A buffer layer 3, the buffer layer 3 is arranged inside the box body 1, the buffer layer 3 is provided with a plurality of accommodating holes of different sizes and a probe groove 31, and the plurality of accommodating holes are respectively used to place reagent tubes 6 of different diameters;

[0051] At least two groups of lifting mechanisms, the two groups of lifting mechanisms are arranged between the buffer layer 3 and the bottom plate of the box body 1;

[0052] At least two traction ropes 5 , one end of the two traction ropes 5 are respectively connected to the two lifting mechanisms, and the other end of the two traction ropes 5 are respectively connected to the box cover 2 .

[0053] In the utility model, the buffer layer 3 is used to store the reagent tube 6 and the probe, and the buffer layer 3 is placed in the box body 1, and then the box cover 2 is connected to the lifting mechanism through the traction rope 5, so that the box cover 2 and the lifting mechanism are linked, and the lifting mechanism can be driven to lift the buffer layer 3 when the box cover 2 is opened, so that the buffer layer 3 protrudes from the box body 1, which is convenient for operators to take the corresponding reagent tube 6 and probe, and when the box cover 2 is closed, the lifting mechanism is reset, so that the buffer layer 3 falls back into the box body 1, so as to protect the reagent tube 6. When the box cover 2 is opened, the buffer layer 3 is synchronously lifted to protrude the reagent tube 6 from the box body 1, saving operation time, and quickly and conveniently taking out the required reagent tube 6.

[0054] Lifting mechanism embodiment 1 4 Figure 2 and Figure 3 As shown, the lifting mechanism includes a hinge column 41 provided on the bottom plate of the box body 1, and a push rod 42 hinged to the hinge column 41, and a slide groove is provided at a position of the bottom plate of the box body 1 corresponding to the push rod 42, and a slider 43 is provided in the slide groove, and the outer side surface of the slider 43 is connected to the inner wall of the box body 1 through a spring 44;

[0055] The height of the slider 43 is greater than the height of the hinge column 41;

[0056] One end of the traction rope 5 is connected to the slider 43. When the traction rope 5 is pulled by the box cover 2, it drives the slider 43 to move toward the hinge column 41, lifts the top rod 42, and then when the box cover 2 is closed, the slider 43 is reset under the action of the spring 44. The spring 44 here is preferably a tension spring; in addition, the top of the slider 43 is a round head, so that the friction between the top of the slider 43 and the top rod 42 is smaller.

[0057] Lifting mechanism embodiment 2 4 'as shown Figure 4 and Figure 5 As shown, the lifting mechanism includes two hinged columns arranged on the bottom plate of the box body 1, and the hinged column 41 close to the opening of the box cover 2 is a fixed column, and the hinged column away from the opening of the box cover 2 is a sliding column 45, and the sliding column 45 is arranged in a slide groove of the bottom plate of the box body 1, and the slide groove here is the same as the slide groove of the first embodiment;

[0058] Two connecting rods 46 are further provided between the two hinged columns, the outer ends of the two connecting rods 46 are hinged to the hinged columns respectively, and the inner ends of the two connecting rods 46 are hinged to each other;

[0059] A spring 44 is also provided between the two hinged columns, and the spring 44 is preferably a compression spring;

[0060] The traction rope 5 is connected to the sliding hinge column.

[0061] The overall function of the above-mentioned two lifting mechanisms is to drive the slider 43 or the sliding hinged column to contract through the traction rope 5, lift the top rod 42 or fold and lift the connecting rod 46, so as to achieve the function of lifting the buffer layer 3. The only difference between the two is that within the same stroke, the lifting height of the folding connecting rod 46 is greater, which can make the buffer layer 3 protrude more from the box body 1.

[0062] like Figure 6 As shown, a rope groove is provided at a position of the buffer layer 3 corresponding to the traction rope 5 .

[0063] The rope groove is used to make room for the traction rope 5 and to limit the traction rope 5, ensuring that the traction rope 5 is in the same vertical plane relative to the lifting mechanism, so that the direction of the pulling force applied by the traction rope 5 is consistent with the direction of the slide groove, reducing the pulling force, and allowing the box cover 2 to be opened more effortlessly.

[0064] On the basis of the above technical solution, one side of the box cover 2 and one side of the box body 1 are connected by a hinge;

[0065] Alternatively, the box cover 2 and the box body 1 are integrally injection-molded, and a bending belt is provided between the box cover 2 and the box body 1 .

[0066] The above structures show different composition forms of the box body 1 and the box cover 2.

[0067] On the basis of the above technical solution, the box cover 2 is connected to the other side of the box body 1 through a buckle, a hook or a locking piece.

[0068] The locking structure of the box cover 2 and the box body 1 is shown.

[0069] On the basis of the above technical solution, the material of the buffer layer 3 is thermoplastic elastomer.

[0070] Thermoplastic elastomer has elasticity and softness similar to rubber and can maintain its elastic form at room temperature; this property enables the buffer layer 3 to adapt to the box body 1, and the size of the accommodating hole can be smaller than the size of the reagent tube to achieve the purpose of wrapping the reagent tube 6; in addition, the thermoplastic elastomer also has the characteristics of wear resistance, weather resistance, chemical stability and colorability.

[0071] On the basis of the above technical solution, a clearance notch is further provided on the top surface of the probe groove 31 .

[0072] The clearance notch can reduce the distance between the top surface and the bottom of the probe groove 31, making it more convenient to take out the probe.

[0073] Several reagent tubes 6 are respectively used to place different materials for genetic testing. In addition to marking these reagent tubes 6 with labels, they can also be distinguished by designing reagent tubes 6 of different sizes and reagent tube covers of different colors.

[0074] For example, a fluorescent quantitative RT-PCR kit for melanocortin 1 receptor (MC1R) gene is provided, wherein the kit contains five reagent tubes 6, which are respectively a reagent tube containing SYBR Green Master hot start fluorescent PCR mixture, a reagent tube containing specific forward and reverse primers for chicken gene expression, a reagent tube containing specific forward and reverse primers for chicken internal reference gene expression, a reagent tube containing dNTP, and a reagent tube containing sterilized DEPC water.

[0075] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, ordinary technicians in this field should understand that they can still modify the technical solutions recorded in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not make the essence of the corresponding technical solution deviate from the scope of the technical solution of the embodiments of the utility model.

Claims

1. A fluorescent quantitative RT-PCR kit with a built-in cushion layer that automatically lifts, characterized in that: include: Box body; A box cover, which is arranged on the top surface of the box body and can rotate along one side of the box body; A buffer layer, the buffer layer is arranged inside the box body, the buffer layer is provided with a plurality of accommodating holes of different sizes and a probe groove, and the plurality of accommodating holes are respectively used to place reagent tubes of different diameters; At least two groups of lifting mechanisms, wherein the two groups of lifting mechanisms are arranged between the buffer layer and the bottom plate of the box body; At least two traction ropes, one end of the two traction ropes is respectively connected to the two lifting mechanisms, and the other end is respectively connected to the box cover.

2. The fluorescent quantitative RT-PCR kit with built-in cushion layer automatically lifted according to claim 1, characterized in that: The lifting mechanism includes a hinged column arranged on the bottom plate of the box body, and a top rod hinged to the hinged column, and a slide groove is provided at a position of the bottom plate of the box body corresponding to the top rod, a slider is provided in the slide groove, and the outer side surface of the slider is connected to the inner wall of the box body through a spring; And the height of the sliding block is greater than the height of the hinge column; One end of the traction rope is connected to the slider.

3. The fluorescent quantitative RT-PCR kit with built-in cushion layer automatically lifted according to claim 1, characterized in that: The lifting mechanism comprises two hinged columns arranged on the bottom plate of the box body, wherein the hinged column close to the opening of the box cover is a fixed column, and the hinged column away from the opening of the box cover is a sliding column, and the sliding column is arranged in a sliding groove of the bottom plate of the box body; Two connecting rods are also provided between the two hinged columns, the outer ends of the two connecting rods are hinged to the hinged columns respectively, and the inner ends of the two connecting rods are hinged to each other; A spring is also provided between the two hinged columns; The traction rope is connected to the sliding hinge column.

4. The fluorescent quantitative RT-PCR kit with built-in cushion layer automatically lifted according to claim 2 or 3, characterized in that: The buffer layer is provided with a rope groove at a position corresponding to the traction rope.

5. The fluorescent quantitative RT-PCR kit with built-in cushion layer automatically lifted according to claim 4, characterized in that: One side of the box cover and one side of the box body are connected by a hinge; Alternatively, the box cover and the box body are integrally injection-molded, and a bending belt is provided between the box cover and the box body.

6. The fluorescent quantitative RT-PCR kit with built-in cushion layer automatically lifted according to claim 5, characterized in that: The box cover is connected to the other side of the box body through a buckle, a hook or a locking piece.

7. The fluorescent quantitative RT-PCR kit with built-in cushion layer automatically lifted according to claim 1, characterized in that: The material of the buffer pad layer is thermoplastic elastomer.

8. The fluorescent quantitative RT-PCR kit with built-in cushion layer automatically lifted according to claim 1, characterized in that: The top surface of the probe groove is also provided with a clearance notch.

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