CCK8 cell proliferation detection kit

Abstract

The utility model relates to the technical field of kits, in particular to a CCK8 cell proliferation detection kit. According to the technical scheme, the kit comprises a kit main body and an upper cover in threaded connection with the top of the kit main body, wherein a splash-proof assembly for preventing reagents from splashing is mounted at the bottom of the upper cover; the splash-proof assembly comprises a connecting rod fixedly connected to the inner wall of the upper cover, a plurality of splash-proof pieces are connected to the bottom of the connecting rod in a circular dispersing mode, and a sealing strip is connected to the upper surface of one side of each splash-proof piece. The bottom of the kit main body is also detachably connected with a suction cup. The reagent box is novel and compact in structure, has a reagent splash-proof effect, avoids reagent waste, reduces the risk of exposure of operators, is high in transfer stability, and has certain practicability.

Description

Technical Field

[0001] The utility model relates to the technical field of kits, in particular to a CCK8 cell proliferation detection kit. Background Art

[0002] CCK-8 reagent is reduced to a highly water-soluble yellow formazan product by dehydrogenase in the cell mitochondria under the action of the electron carrier 1-methoxy-5-methylphenazine dimethyl sulfate. The amount of formazan generated is proportional to the number of living cells. CCK-8 Cell Proliferation Detection Kit is a kit used to evaluate cell proliferation.

[0003] The CCK-8 reagent is easy to use, eliminates the need for washing cells, does not require radioactive isotopes and organic solvents; has fast detection; 3. High sensitivity, even for measuring lower cell densities; has better repeatability than the MTT method; has low cytotoxicity; is a bottle of solution, does not need to be prefabricated, and is ready to use. However, the CCK-8 reagent kit used for cell proliferation detection on the market is stored in a threaded bottle body. Since the CCK-8 reagent is a liquid, when the stored reagent bottle body is opened, there is a ring between the upper cover of the bottle body and the bottle body, and the liquid will splash when the knob is turned hard, and the operator's hands are easily contaminated. At the same time, the capacity of each reagent kit is limited, and splashing will cause a waste of reagents. The existing reagent kit does not have a splash-proof function. In view of the above reasons, the present application proposes a CCK8 cell proliferation detection kit with a knob splash-proof function. Utility Model Content

[0004] The utility model aims to solve the problems existing in the background technology and proposes a CCK8 cell proliferation detection kit with a knob splash-proof function when taking out the reagent for use.

[0005] The technical solution of the utility model: a CCK8 cell proliferation detection kit comprises a kit body and an upper cover threadedly connected to the top of the kit body, wherein a splash-proof component for preventing reagent splashing is installed at the bottom of the upper cover;

[0006] The splash-proof assembly comprises a connecting rod fixedly connected to the inner wall of the upper cover, the bottom of the connecting rod is circularly dispersedly connected to a plurality of splash-proof sheets, and the upper surface of one side of each splash-proof sheet is connected to a sealing strip;

[0007] The bottom of the reagent kit body is also detachably connected with a suction cup.

[0008] Optionally, one end of the connecting rod away from the upper cover is fixedly connected to a rubber disk, and the outer ring of the rubber disk is provided with a plurality of notches in an array along its circumference.

[0009] Optionally, the gap between two adjacent notches is used for fixing and connecting the splash-proof plate.

[0010] Optionally, the upper surface of the splash-proof sheet is also provided with a plurality of buffer grooves along its curvature direction.

[0011] Optionally, a liquid collection port is provided on the top of the reagent kit body, and the outermost edge of the circular surface formed by the plurality of splash-proof sheets contacts the inner wall of the reagent kit body.

[0012] Optionally, a snap-in opening is provided at the bottom of the reagent kit body, a snap-in block is connected to the top of the suction cup, and the reagent kit body and the suction cup are assembled and connected via the snap-in block and the snap-in opening.

[0013] Optionally, the main body of the kit is an opaque bottle.

[0014] Optionally, the outer ring of the upper cover has a plurality of vertically arranged anti-slip grooves.

[0015] Compared with the prior art, the utility model has the following beneficial technical effects:

[0016] The utility model assembles a plurality of splash-proof sheets to form a circular surface with an outer ring that fits the inner wall of the reagent box body, thereby reducing the shaking amplitude of the reagent to a certain extent. By rotating and lifting the upper cover, the splash-proof sheet is folded and compressed in combination with the liquid collection port to deform, thereby avoiding large shaking caused by twisting the upper cover forcefully, and preventing the reagent from splashing out of the reagent box body.

[0017] The utility model provides a suction cup so that the reagent box body is stably sucked to the placement surface when placed, thereby further improving the reagent transportation stability;

[0018] In summary, the utility model has a novel and compact structure, has a reagent splash-proof effect, avoids reagent waste, reduces the risk of operator exposure, has high transportation stability, and has certain practicality; BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 A schematic diagram of the main structure of the utility model is given;

[0020] Figure 2 Provide a schematic diagram of the decomposition structure of the utility model;

[0021] Figure 3 A schematic diagram of the partial assembly structure of the splash-proof sheet and the rubber disc is given;

[0022] Figure 4 A schematic diagram of the splash guard from another perspective is given.

[0023] Reference numerals:

[0024] 1. Test kit body; 11. Card loading port; 12. Liquid extraction port;

[0025] 2. Upper cover; 21. Anti-slip pattern;

[0026] 3. Suction cup; 31. Card-mounting block;

[0027] 4. splash-proof assembly; 41. connecting rod; 42. rubber disc; 421. notch; 43. splash-proof sheet; 431. buffer groove; 432. sealing strip. DETAILED DESCRIPTION

[0028] The technical solution of the present disclosure will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present disclosure, rather than all of the embodiments.

[0029] The components of the embodiments of the present disclosure generally described and shown in the drawings herein may be arranged and designed in a variety of different configurations. Therefore, the following detailed description of the embodiments of the present disclosure provided in the drawings is not intended to limit the scope of the present disclosure claimed, but merely represents selected embodiments of the present disclosure.

[0030] Based on the embodiments in the present disclosure, all other embodiments obtained by ordinary technicians in the field without making any creative work shall fall within the scope of protection of the present disclosure.

[0031] In the description of the present disclosure, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present disclosure and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present disclosure.

[0032] In the description of the present disclosure, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present disclosure can be understood according to specific circumstances.

[0033] Example 1

[0034] like Figure 1-4As shown, the CCK8 cell proliferation detection kit proposed by the utility model includes a kit body 1 and an upper cover 2 threadedly connected to the top thereof, the outer ring of the upper cover 2 has a plurality of vertically arranged anti-slip grooves 21, and the arrangement of the plurality of anti-slip grooves 21 makes the rotation of the upper cover 2 more labor-saving and has an anti-slip effect, the kit body 1 is an opaque bottle body, and a splash-proof component 4 for preventing reagent splashing is installed at the bottom of the upper cover 2;

[0035] The splash-proof assembly 4 includes a connecting rod 41 fixedly connected to the inner wall of the upper cover 2, and the bottom of the connecting rod 41 is circularly dispersed and connected with a plurality of splash-proof sheets 43, and the splash-proof sheets 43 and the rubber disc 42 are made of soft silicone material. A liquid taking port 12 is provided on the top of the reagent box body 1, and the liquid taking port 12 is used to pull out the splash-proof assembly 4 and take the reagent CCK8. The outermost edge of the circular surface composed of the plurality of splash-proof sheets 43 contacts the inner wall of the reagent box body 1. When taking the reagent box body 1, the disk-shaped splash-proof sheets 43 will prevent the liquid from shaking greatly, and reduce the probability of the liquid splashing and getting on the inner wall of the upper cover 2;

[0036] like Figure 3 and Figure 4 As shown, the upper surface of the splash-proof sheet 43 is also provided with a plurality of buffer grooves 431 along its curvature direction, and a sealing strip 432 is connected to the upper surface of one side of each splash-proof sheet 43, and the gaps between the plurality of splash-proof sheets 43 are shielded by the sealing strip 432;

[0037] A rubber disk 42 is fixedly connected to one end of the connecting rod 41 away from the upper cover 2. The outer ring of the rubber disk 42 has a plurality of notches 421 arranged along its circumferential array. The notches 421 are used to provide space for the splash-proof sheet 43 to be rolled up in an umbrella shape when the splash-proof sheet 43 is pulled out of the liquid inlet 12. The gap between two adjacent notches 421 is used for fixed connection of the splash-proof sheet 43.

[0038] In this embodiment, when in use, the knob of the upper cover 2 is turned so that the upper cover 2 is separated from the top of the reagent box body 1. At this time, the disk composed of the splash-proof sheet 43 is still located in the reagent box body 1, and the reagent will not splash out of the outside of the reagent box body 1 due to turning the knob forcefully. After the knob is turned, the upper cover 2 is pulled. When the splash-proof sheet 43 passes the liquid collection port 12, since there are multiple splash-proof sheets 43 and buffer grooves 431 are provided on the splash-proof sheets 43, the multiple splash-proof sheets 43 will be bent and folded in an umbrella state, which makes it convenient to take the splash-proof assembly 4 out of the outside of the reagent box body 1 as a whole.

[0039] Example 2

[0040] like Figure 1 and Figure 2As shown, based on Example 1, the bottom of the reagent box body 1 is also detachably connected with a suction cup 3, a clamping port 11 is provided at the bottom of the reagent box body 1, and a clamping block 31 is connected to the top of the suction cup 3. The reagent box body 1 and the suction cup 3 are assembled and connected by the clamping block 31 and the clamping port 11. The suction cup 3 in this embodiment is an optional installation. When the reagent box body 1 needs to be transported or needs to be stably stored, the clamping block 31 is aligned with the clamping port 11 and clamped, and the reagent box body 1 is stably adsorbed to the placement surface through the suction cup 3.

[0041] The above-mentioned specific embodiments are only several optional embodiments of the present invention. Based on the technical solution of the present invention and the relevant inspiration of the above-mentioned embodiments, those skilled in the art can make various alternative improvements and combinations to the above-mentioned specific embodiments.

Claims

1. A CCK8 cell proliferation detection kit, comprising a kit body (1) and an upper cover (2) threadedly connected to the top of the kit body, characterized in that: A splash-proof component (4) for preventing reagents from splashing is installed at the bottom of the upper cover (2); The splash-proof assembly (4) comprises a connecting rod (41) fixedly connected to the inner wall of the upper cover (2); the bottom of the connecting rod (41) is circularly dispersedly connected to a plurality of splash-proof sheets (43); and the upper surface of one side of each splash-proof sheet (43) is connected to a sealing strip (432); The bottom of the reagent kit body (1) is also detachably connected to a suction cup (3).

2. The CCK8 cell proliferation detection kit according to claim 1, characterized in that: One end of the connecting rod (41) away from the upper cover (2) is fixedly connected to a rubber disk (42), and the outer ring of the rubber disk (42) is provided with a plurality of notches (421) in an array along its circumference.

3. The CCK8 cell proliferation detection kit according to claim 2, characterized in that: The gap between two adjacent notches (421) is used for fixed connection of the splash-proof sheet (43).

4. The CCK8 cell proliferation detection kit according to claim 1, characterized in that: The upper surface of the splash-proof sheet (43) is also provided with a plurality of buffer grooves (431) along the arc direction thereof.

5. The CCK8 cell proliferation detection kit according to claim 1, characterized in that: A liquid taking port (12) is provided on the top of the reagent box body (1), and the outermost edge of the circular surface formed by the plurality of splash-proof sheets (43) contacts the inner wall of the reagent box body (1).

6. The CCK8 cell proliferation detection kit according to claim 1, characterized in that: The bottom of the reagent box body (1) is provided with a snap-in opening (11), the top of the suction cup (3) is connected with a snap-in block (31), and the reagent box body (1) and the suction cup (3) are assembled and connected via the snap-in block (31) and the snap-in opening (11).

7. The CCK8 cell proliferation detection kit according to claim 1, characterized in that: The reagent kit body (1) is an opaque bottle.

8. The CCK8 cell proliferation detection kit according to claim 1, characterized in that: The outer ring of the upper cover (2) has a plurality of vertically arranged anti-slip grooves (21).

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