Preparation device of stem cell culture solution for treating arthritis

Abstract

The utility model belongs to the technical field of cell culture, particularly relates to a stem cell culture solution preparation device for treating arthritis, and provides the following scheme aiming at the problems that when cells are cultured, the culture solution and the cells are difficult to mix and stir, so that the condition that the culture solution is not uniformly mixed easily occurs, and the cell proliferation is influenced. Comprising a device body, a culture solution stirring assembly and a lifting mounting assembly, the culture solution stirring assembly comprises a stirring motor, the stirring motor is fixedly installed at the top of the interior of the device body, the output end of the stirring motor is fixedly connected with a stirring shaft, and a stirring propeller is welded to the surface of the stirring shaft. The culture solution stirring assembly is arranged, the stirring motor is started to drive the stirring shaft to rotate, then the stirring barrel is driven to rotate, a culture solution is uniformly mixed and stirred through the stirring rod, and the culture solution is turned over up and down through the stirring propeller, so that the culture solution is more uniform.

Description

Technical Field

[0001] The utility model relates to a device for preparing a stem cell culture solution for treating arthritis, in particular to a device for preparing a stem cell culture solution for treating arthritis, and belongs to the technical field of cell culture. Background Art

[0002] Arthritis mainly involves the hips, knees, spine, and interdigital joints. The most common symptom of arthritis is pain in the affected joints after repeated use. Joint pain is usually worse later in the day. Affected joints may swell, become hot, and creak. Joints can also become painful and stiff after long periods of inactivity. In severe arthritis, the complete loss of cartilage pads causes friction between bones, causing pain at rest or pain with limited movement. With the continuous development of stem cell research, more therapeutic drugs are being explored in the field of arthritis, among which cell culture is an indispensable process, and cell culture is inseparable from culture medium, which requires the use of a stem cell culture medium preparation device for the treatment of arthritis.

[0003] The patent specification with announcement number CN212025372U discloses a stem cell culture device, including a box body, a sealing cover, a culture dish, a pull rod, and a sterilization device. An opening is provided at the upper end of the box body, and the sealing cover can seal the opening at the upper end of the box body. A plurality of guide plates are protruding above the inner wall of the box body. The culture dish is arranged inside the box body, and an edge is protruding outward from the upper end of the culture dish. The lower end of the pull rod supports the edge through a support plate, and the upper end of the pull rod passes through the guide plate. The sterilization device is fixed at the lower part of the box body, and the sterilization device can sterilize the gas inside the box body. The utility model is easy to operate, creates a relatively closed environment, ensures the carbon dioxide concentration inside the environment, and can sterilize the gas circulating inside.

[0004] However, in the implementation of relevant technologies, it was found that the stem cell culture device designed above has the following problems: when culturing stem cells for proliferation, culture medium needs to be added to ensure cell nutrition. In the above technical scheme, when culturing cells, it is difficult to mix and stir the culture medium and cells, which leads to uneven mixing of the culture medium and affects cell proliferation. In view of this, a device for preparing stem cell culture medium for treating arthritis is provided to overcome the above defects. Utility Model Content

[0005] The utility model provides a device for preparing stem cell culture fluid for treating arthritis in order to solve the problem that it is difficult to mix and stir the culture fluid with the cells during cell culture, resulting in uneven mixing of the culture fluid and affecting cell proliferation.

[0006] The utility model achieves the above-mentioned purpose through the following technical solutions: A device for preparing a culture solution of stem cells for treating arthritis, comprising a device body, and a culture solution stirring component and a lifting and mounting component located inside the device body;

[0007] The culture liquid stirring assembly includes a stirring motor, which is fixedly installed on the inner top of the device body, the output end of the stirring motor is fixedly connected to a stirring shaft, the surface of the stirring shaft is welded with a stirring propeller, the outside of the stirring shaft is connected to a stirring barrel through a connecting rod, the surface of the stirring barrel is provided with a plurality of stirring rods, and the surface of the stirring barrel is provided with a plurality of through grooves;

[0008] As a further solution of the utility model: the lifting installation assembly includes a screw motor, the screw motor is fixedly installed inside the bottom of the device body, the output end of the screw motor is fixedly connected to a screw, and the surface of the screw is threadedly connected to a guide assembly seat.

[0009] As a further solution of the utility model: a mounting plate is fixedly mounted on one side of the guide assembly seat, and a mounting block is slidably connected inside the mounting plate.

[0010] As a further solution of the utility model: a culture dish is fixedly connected to one side of the mounting block.

[0011] As a further solution of the utility model: a sealed door is installed on the surface of the device body through a hinge.

[0012] As a further solution of the utility model: an observation window is provided on the surface of the sealed warehouse door.

[0013] As a further solution of the utility model: the culture dish is lifted and lowered inside the device body through the screw motor, the screw and the guide assembly seat.

[0014] The beneficial effects of the utility model are:

[0015] The utility model provides a lifting installation component, which can slide the culture dish for cultivating stem cells into the interior of the installation plate through the installation block, and limit and fix the culture dish. Then, the screw motor is started to drive the screw to rotate, and the guide assembly seat, the installation plate and the culture dish can be driven to move upward. This arrangement makes it easy for the stirring rod to penetrate into the interior of the culture dish, and is convenient for flexible adjustment of the stirring height, while improving the stability of the placement of the culture dish.

[0016] The utility model sets a culture liquid stirring component, starts the stirring motor to drive the stirring shaft to rotate, and then drives the stirring barrel to rotate, so that the culture liquid is evenly mixed and stirred by the stirring rod, and the culture liquid is turned upside down by the stirring propeller, so that the culture liquid can be made more uniform. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0018] Figure 2 It is a schematic diagram of the internal structure of the device body of the utility model;

[0019] Figure 3 This is a schematic diagram of the structure of the culture solution stirring assembly of the utility model;

[0020] Figure 4 It is a structural schematic diagram of the lifting and installation assembly of the utility model.

[0021] In the figure: 1. Device body; 2. Observation window; 3. Sealed compartment door; 4. Culture solution stirring assembly; 401. Stirring shaft; 402. Stirring rod; 403. Stirring propeller; 404. Stirring barrel; 405. Through slot; 5. Lifting installation assembly; 501. Mounting plate; 502. Mounting block; 503. Guide assembly seat; 504. Screw; 505. Screw motor; 6. Culture dish. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model. Embodiment 1

[0023] like Figures 1 to 4 As shown, a device for preparing a stem cell culture solution for treating arthritis includes a device body 1, and a culture solution stirring component 4 and a lifting and mounting component 5 located inside the device body 1;

[0024] The culture liquid stirring component 4 includes a stirring motor, which is fixedly installed on the internal top of the device body 1. The output end of the stirring motor is fixedly connected to a stirring shaft 401, and a stirring propeller 403 is welded on the surface of the stirring shaft 401. The outside of the stirring shaft 401 is connected to a stirring barrel 404 through a connecting rod. The surface of the stirring barrel 404 is provided with several groups of stirring rods 402, and the surface of the stirring barrel 404 is provided with multiple groups of through grooves 405. Through the culture liquid stirring component 4, the stirring motor is started to drive the stirring shaft 401 to rotate, and then the stirring barrel 404 is driven to rotate, so that the culture liquid is evenly mixed and stirred through the stirring rod 402, and the culture liquid is flipped up and down through the stirring propeller 403, so that the culture liquid can be more uniform. Embodiment 2

[0025] In addition to all the technical features of the first embodiment, this embodiment also includes:

[0026] The lifting and mounting assembly 5 includes a screw motor 505 , which is fixedly mounted inside the bottom of the device body 1 . The output end of the screw motor 505 is fixedly connected to a screw 504 , and the surface of the screw 504 is threadedly connected to a guide assembly seat 503 . Embodiment 3

[0027] In addition to all the technical features of the first embodiment, this embodiment also includes:

[0028] A mounting plate 501 is fixedly mounted on one side of the guide assembly seat 503 , and a mounting block 502 is slidably connected inside the mounting plate 501 . This arrangement can improve the stability of the placement of the culture dish 6 .

[0029] A culture dish 6 is fixedly connected to one side of the mounting block 502 , and the culture dish 6 is used for culturing stem cells.

[0030] A sealed door 3 is installed on the surface of the device body 1 through a hinge, and the sealed door 3 can prevent dust and bacteria from entering the device body 1 and causing contamination to the cultured cells.

[0031] An observation window 2 is provided on the surface of the sealed compartment door 3 , and the observation window 2 is convenient for observing the internal situation of the device body 1 .

[0032] The culture dish 6 is lifted and lowered inside the device body 1 by means of the screw motor 505, the screw 504 and the guide assembly seat 503. This arrangement allows the stirring rod 402 to penetrate into the interior of the culture dish 6, facilitates flexible adjustment of the stirring height, and improves the stability of the placement of the culture dish 6.

[0033] Working principle: When using the device for preparing stem cell culture fluid for treating arthritis, the culture dish 6 for cultivating stem cells can be slid into the interior of the mounting plate 501 through the provided lifting installation component 5, and the culture dish 6 is limited and fixed. Then, the screw motor 505 is started to drive the screw 504 to rotate, so as to drive the guide assembly seat 503, the mounting plate 501 and the culture dish 6 to move upward. This arrangement makes it easy for the stirring rod 402 to penetrate into the interior of the culture dish 6, and is convenient for flexible adjustment of the stirring height, while improving the stability of the placement of the culture dish 6; through the provided culture fluid stirring component 4, the stirring motor is started to drive the stirring shaft 401 to rotate, and then the stirring barrel 404 is driven to rotate, so that the culture fluid is evenly mixed and stirred through the stirring rod 402, and the culture fluid is flipped up and down through the stirring propeller 403, so that the culture fluid can be more uniform.

[0034] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

[0035] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A device for preparing stem cell culture fluid for treating arthritis, characterized in that: It comprises a device body (1), and a culture liquid stirring component (4) and a lifting and mounting component (5) located inside the device body (1); The culture liquid stirring assembly (4) comprises a stirring motor, which is fixedly mounted on the inner top of the device body (1); the output end of the stirring motor is fixedly connected to a stirring shaft (401); a stirring propeller (403) is welded on the surface of the stirring shaft (401); the outside of the stirring shaft (401) is connected to a stirring barrel (404) via a connecting rod; a plurality of stirring rods (402) are arranged on the surface of the stirring barrel (404); and a plurality of through grooves (405) are provided on the surface of the stirring barrel (404).

2. The device for preparing stem cell culture fluid for treating arthritis according to claim 1, characterized in that: The lifting and mounting assembly (5) comprises a screw motor (505), wherein the screw motor (505) is fixedly mounted inside the bottom of the device body (1), an output end of the screw motor (505) is fixedly connected to a screw (504), and a surface of the screw (504) is threadedly connected to a guide assembly seat (503).

3. The device for preparing stem cell culture fluid for treating arthritis according to claim 2, characterized in that: A mounting plate (501) is fixedly mounted on one side of the guide assembly seat (503), and a mounting block (502) is slidably connected inside the mounting plate (501).

4. The device for preparing stem cell culture fluid for treating arthritis according to claim 3, characterized in that: A culture dish (6) is fixedly connected to one side of the mounting block (502).

5. The device for preparing stem cell culture fluid for treating arthritis according to claim 1, characterized in that: A sealed compartment door (3) is mounted on the surface of the device body (1) via a hinge.

6. The device for preparing stem cell culture fluid for treating arthritis according to claim 5, characterized in that: An observation window (2) is provided on the surface of the sealed compartment door (3).

7. The device for preparing stem cell culture fluid for treating arthritis according to claim 4, characterized in that: The culture dish (6) is lifted and lowered inside the device body (1) by means of a screw motor (505), a screw (504) and a guide assembly seat (503).

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