Artificial feeding device for mouthpart piercing and sucking insects

Abstract

The utility model relates to an artificial feeding device for mouthpart piercing and sucking insects. The artificial feeding device comprises a first plastic transparent pipeline and a second plastic transparent pipeline, the upper end of the first plastic transparent pipeline is coated with a first thin drawing film; the upper end of the second plastic transparent pipeline is coated with a second thin drawing film; the lower surface of the first plastic transparent pipeline is in contact with the upper surface of the second thin drawing film; connecting devices are arranged on the outer circumferential side surface of the lower end of the first plastic transparent pipeline and the outer circumferential side surface of the upper end of the second plastic transparent pipeline, and are used for fixing the first plastic transparent pipeline above the second thin drawing film and sealing the first plastic transparent pipeline; the device disclosed by the utility model is simple and convenient to operate, and is favorable for improving the feeding efficiency and experimental operation of mouthpart piercing and sucking insects.

Description

Technical Field

[0001] The utility model belongs to the technical field of bioengineering, in particular to an artificial breeding device for insects with piercing and sucking mouthparts. Background Art

[0002] Piercing-sucking insects are insects that feed on plant sap or animal blood. They have mouthparts that can penetrate the host and suck the host's fluid. Piercing-sucking pests often suck plant sap, causing the branches, leaves and flowers to curl, or even the whole plant to wither or die, which is extremely harmful to garden plants and crops. In recent years, the incidence of piercing-sucking pests such as rice planthoppers, cotton aphids, blind bugs, and whiteflies has been increasing year by year in China. With the rise of IPM (Integrated Pest Management), a variety of pest control technologies have emerged, and more and more researchers have turned their attention to insect toxicology, insect molecular biology, insect ecology, insect behavior and other disciplines. By raising these insects, researchers can more conveniently carry out laboratory breeding and operation to better understand their biological characteristics, life history and ecological habits, so as to develop effective pest control strategies and reduce crop losses.

[0003] Based on the above problems, the existing patent CN201920245565.4 proposes a device for biological evaluation of piercing-sucking insects, which is provided by setting two layers of Pula membranes at the upper opening of the feeding shell, and feeding the piercing-sucking insects by setting insect feed between the two layers of Pula membranes. Although this method solves the problems existing in traditional biological evaluation, it places less feed directly between the two layers of Pula membranes, and the insect feeding time is short, which is not conducive to long-term observation and experimentation of piercing-sucking insects. Secondly, because the two layers of Pula membranes are almost in a fitted state, piercing-sucking insects can easily pierce the two layers of Pula membranes at the same time when eating feed, causing external air to enter the feed storage area, causing the feed to deteriorate, and then causing the device to be scrapped and replaced, with low feeding efficiency and short service life. Utility Model Content

[0004] In order to solve the problems existing in the background technology, the present invention provides an artificial breeding device for piercing-sucking insects with mouthparts to improve the breeding efficiency of piercing-sucking insects with mouthparts and facilitate experimental operations, including: a first plastic transparent pipe and a second plastic transparent pipe; the upper end of the first plastic transparent pipe is covered with a first thin film; the upper end of the second plastic transparent pipe is covered with a second thin film; the lower side of the first plastic transparent pipe is in contact with the upper side of the second thin film; a connecting device is provided on the outer circumferential side of the lower end of the first plastic transparent pipe and the outer circumferential side of the upper end of the second plastic transparent pipe, which is used to fix the first plastic transparent pipe above the second thin film and seal it; the lower end of the second plastic transparent pipe is provided with absorbent cotton for sealing.

[0005] Preferably, a nutrient solution for piercing-sucking insects is provided in the first plastic transparent pipe, between the first thin film and the second thin film; the piercing-sucking insects are raised in the second plastic transparent pipe, between the second thin film and the absorbent cotton.

[0006] Preferably, the connecting device includes but is not limited to: an injection tube, an adhesive tape, an adhesive strip, an adhesive plaster or a thin film.

[0007] Preferably, the first thin film or the second thin film is a Parafilm sealing film.

[0008] Preferably, the inner diameter and outer diameter of the first plastic transparent pipe and the second plastic transparent pipe are the same, and the length of the first plastic transparent pipe is shorter than the length of the second plastic transparent pipe.

[0009] Preferably, the outer circumferential side of the second plastic transparent pipe is wrapped with black cardboard; and an artificial climate light is arranged above the first plastic transparent pipe.

[0010] Preferably, a supporting structure is fixedly provided at the upper end of the second plastic transparent pipe; the supporting structure is a circular supporting plate, and a plurality of through holes are provided on one side surface of the supporting plate.

[0011] Preferably, the outer diameter of the support plate is the same as the outer diameter of the second plastic transparent pipe; the lower side of the support plate is fixedly connected to the upper side of the second plastic transparent pipe; the lower side of the second thin film is in contact with the upper side of the support plate.

[0012] Preferably, the outer diameter of the support plate is adapted to the inner diameter of the second plastic transparent pipe; the outer circumferential side of the support plate is fixedly connected to the inner circumferential side of the second plastic transparent pipe; the top of the support plate and the top of the second plastic transparent pipe are in the same plane; the bottom of the second thin film is in contact with the top of the support plate.

[0013] Preferably, the thickness of the support plate is less than 100 μm.

[0014] The utility model has at least the following beneficial effects

[0015] The utility model patent can be used for observation, feeding and experimental operation of piercing-sucking mouthpart insects. The directional feeding efficiency of insects can be adjusted by black cardboard (added or cancelled) and light outside the second plastic transparent pipe. By replacing the nutrient solution medicine in the first plastic transparent pipe, physiological research on piercing-sucking mouthpart insects under different environments can be realized. The utility model only needs to open the first plastic transparent pipe or add nutrient solution medicine to the first plastic transparent pipe through a syringe. The operation is simple and can be reused. The utility model device can effectively prevent piercing-sucking mouthpart insects from piercing two layers of sealing films at the same time, resulting in contamination of nutrient solution medicine in the first plastic transparent pipe. At the same time, a large amount of nutrient solution medicine can be added to the first plastic transparent pipe through the device, which increases the feeding time. The device can be reused and has a longer service life. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the structure of Example 1 of the utility model;

[0017] Figure 2 This is a schematic diagram of the structure of Example 2 of the present utility model;

[0018] Figure 3 This is a schematic diagram of the structure of Embodiment 3 of the present utility model;

[0019] Figure 4 This is a schematic diagram of the structure of Embodiment 4 of the present utility model;

[0020] The reference numerals in the figures are:

[0021] 1. a first plastic transparent pipe, 11. a first thin film, 2. a second plastic transparent pipe, 21. a second thin film, 3. a connecting device, 4. cotton wool, 5. black cardboard, 6. an artificial climate lamp, 7. a supporting structure, 8. a through hole. DETAILED DESCRIPTION

[0022] The following describes the implementation of the present invention through specific examples. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific implementations, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the illustrations provided in the following embodiments only illustrate the basic concept of the present invention in a schematic manner. The following embodiments and the features in the embodiments can be combined with each other without conflict.

[0023] Among them, the drawings are only used for illustrative explanations, and they only represent schematic diagrams rather than actual pictures, and should not be understood as limitations on the present utility model. In order to better illustrate the embodiments of the present utility model, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of the actual product. For those skilled in the art, it is understandable that some well-known structures and their descriptions in the drawings may be omitted.

[0024] The same or similar numbers in the drawings of the embodiments of the present invention correspond to the same or similar parts; in the description of the present invention, it should be understood that if the terms "upper", "lower", "left", "right", "front", "back" and the like indicate directions or positional relationships, they are based on the directions or positional relationships shown in the drawings, which are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, the terms describing the positional relationship in the drawings are only used for illustrative purposes and cannot be understood as a limitation on the present invention. For ordinary technicians in this field, the specific meanings of the above terms can be understood according to specific circumstances.

[0025] See also Figure 1 The utility model provides an artificial breeding device for insects with piercing-sucking mouthparts to improve the breeding efficiency of insects with piercing-sucking mouthparts, comprising: a first plastic transparent pipe 1 and a second plastic transparent pipe 2; the upper end of the first plastic transparent pipe 1 is covered with a first thin film 11; the upper end of the second plastic transparent pipe 2 is covered with a second thin film 21; the lower side of the first plastic transparent pipe 1 is in contact with the upper side of the second thin film 21; a connecting device 3 is arranged on the outer circumferential side of the lower end of the first plastic transparent pipe 1 and the outer circumferential side of the upper end of the second plastic transparent pipe 2, which is used to fix the first plastic transparent pipe 1 above the second thin film 21 and seal it; the lower end of the second plastic transparent pipe 2 is provided with an absorbent cotton 4 for sealing.

[0026] In this embodiment, the inner diameter of the second plastic transparent pipe 2 is 2.3 cm, the outer diameter is 2.5 cm, and the length is 15 cm. The lower end of the second plastic transparent pipe 2 is sealed with a moistened cotton ball, and the upper end of the second plastic transparent pipe 2 is sealed with a Parafilm sealing film; the inner diameter of the first plastic transparent pipe 1 is 2.3 cm, the outer diameter is 2.5 cm, and the length is 0.5 cm.

[0027] Preferably, a nutrient solution for piercing-sucking insects is provided in the first plastic transparent tube 1 between the first thin film 11 and the second thin film 21 ; the piercing-sucking insects are raised in the second plastic transparent tube 2 between the second thin film 21 and the absorbent cotton 4 .

[0028] Preferably, the connecting device 3 includes but is not limited to: a liquid injection tube, an adhesive tape, an adhesive strip, an adhesive plaster or a thin film.

[0029] Preferably, the first thin film 11 or the second thin film 21 is made of Parafilm sealing film.

[0030] Preferably, the inner diameter and outer diameter of the first plastic transparent pipe 1 and the second plastic transparent pipe 2 are the same, and the length of the first plastic transparent pipe 1 is shorter than the length of the second plastic transparent pipe 2 .

[0031] See also Figure 2 Preferably, the outer circumferential side of the second plastic transparent pipe 2 is wrapped with black cardboard 5; and an artificial climate lamp 6 is arranged above the first plastic transparent pipe.

[0032] See also Figure 3 and Figure 4 Preferably, a support structure 7 is fixedly provided at the upper end of the second plastic transparent pipe 2; the support structure 7 is a circular support plate, and a plurality of through holes 8 are provided on one side surface of the support plate.

[0033] In this embodiment, the nutrient solution medicine can be supported by providing a support structure 7 at the upper end of the second plastic transparent pipe 2, thereby preventing excessive pressure on the second thin film 21 due to the excessive weight of the nutrient solution medicine, causing the second thin film 21 to deform downward, thereby causing insects with piercing-sucking mouthparts to pierce the second thin film 21 and causing the nutrient solution medicine to flow downward from the second thin film 21.

[0034] See also Figure 3 Preferably, the outer diameter of the support plate is the same as the outer diameter of the second plastic transparent pipe 2; the bottom of the support plate is fixedly connected to the top of the second plastic transparent pipe 2; the bottom of the second thin film 21 is in contact with the top of the support plate.

[0035] See also Figure 4 Preferably, the outer diameter of the support plate is adapted to the inner diameter of the second plastic transparent pipe 2; the outer circumferential side of the support plate is fixedly connected to the inner circumferential side of the second plastic transparent pipe 2; the top of the support plate and the top of the second plastic transparent pipe 2 are in the same plane; the bottom of the second thin film 21 is in contact with the top of the support plate.

[0036] Preferably, the thickness of the support plate is less than 100 μm.

[0037] In the specific implementation, the Parafilm sealing film is cut into a 2.5cm*2.5cm square, and the sealing film is pulled in all directions with both hands to make the Parafilm sealing film become a thinner sealing film of 5cm*5cm, so as to obtain the first thin film 11 and the second thin film 21, and then 1-2mL of brown planthopper artificial feed and tetracycline antibiotic solution are sucked with a pipette, and the antibiotic concentration reaches 200mg / 100ml, and 20 heads of each group of brown planthoppers are placed in the second plastic transparent pipe 2, and then the absorbent cotton ball is wetted with sterile water to block the other end of the glass second plastic transparent pipe 2, and finally the second plastic transparent pipe 2 is wrapped with black opaque cardboard slightly larger than the transparent glass pipe. The light of the artificial climate box is positioned toward the sealing film end of the second plastic transparent pipe 2, and the brown planthopper will move toward the side of the transparent sealing film and feed due to the action of phototaxis, and the antibiotic solution can smoothly enter the intestine of the brown planthopper to inhibit or kill the symbiotic bacteria in the intestine. The Parafilm sealing film of the first plastic transparent pipe 1 is removed within a certain period of time, and artificial feed and antibiotic solution are added to artificially feed the brown planthopper and cultivate a sterile strain.

[0038] In one use of the utility model, firstly, the second thin film 21 is covered on the upper end of the second plastic transparent pipe 2 by using rubber bands, adhesive strips, and adhesive tape, and the first plastic transparent pipe 1 is placed on the second thin film 21, and the first plastic transparent pipe 1 is fixed on the upper end of the second thin film 21 by a connecting device and sealed; after the sealing is completed, insect feed is added into the first plastic transparent pipe 1, and after the addition is completed, the first thin film 11 is covered on the upper end of the first plastic transparent pipe 1 by using rubber bands, adhesive strips, and adhesive tape, and then the piercing and sucking mouthparts insects are put into the second plastic transparent pipe 2, and the lower end of the second plastic transparent pipe 2 is sealed with wetted absorbent cotton 4, and the insects in the second plastic transparent pipe 2 are placed in the second plastic transparent pipe 2. The outer circumferential side of the transparent tube 2 is wrapped with black cardboard 5, and an artificial climate lamp 6 is arranged above the first plastic transparent tube 1. By turning on the artificial climate lamp 6, the phototropism of insects is utilized, so the insects can move toward the first thin film 11 driven by light. The first plastic transparent tube 1 can fix artificial feed and antibiotics. When it is necessary to observe the piercing-sucking mouthparts insects during the breeding process, the staff can remove the black cardboard 5 to observe the piercing-sucking mouthparts insects. When it is necessary to study the physiological responses of the piercing-sucking mouthparts insects under different drugs, it is only necessary to remove the first thin film 11 and add the corresponding nutrient solution to the first plastic transparent tube 1. After the addition is completed, the first thin film 11 is covered.

[0039] In summary, the utility model patent can be used to observe and raise insects with piercing-sucking mouthparts. By replacing the nutrient solution medicine in the first plastic transparent tube, physiological research on insects with piercing-sucking mouthparts under different environments can be realized. The utility model only needs to open the first plastic transparent tube or add nutrient solution medicine to the first plastic transparent tube through a syringe. The operation is simple and can be reused. The device of the utility model can effectively prevent the piercing-sucking mouthparts insects from piercing two layers of sealing films at the same time, resulting in contamination of the nutrient solution medicine in the first plastic transparent tube. At the same time, by adding a large amount of nutrient solution medicine in the first plastic transparent tube through the device, a longer breeding time can be increased. The device can be reused and has a longer service life.

[0040] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

[0041] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model rather than to limit it. Although the utility model has been described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the technical solution of the utility model can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution, which should be included in the scope of the claims of the utility model.

Claims

1. An artificial breeding device for insects with piercing-sucking mouthparts, characterized in that: include: A first plastic transparent pipe (1) and a second plastic transparent pipe (2); the upper end of the first plastic transparent pipe (1) is coated with a first thin film (11); the upper end of the second plastic transparent pipe (2) is coated with a second thin film (21); the lower side of the first plastic transparent pipe (1) is in contact with the upper side of the second thin film (21); a connecting device (3) is provided on the outer circumferential side of the lower end of the first plastic transparent pipe (1) and the outer circumferential side of the upper end of the second plastic transparent pipe (2) for fixing the first plastic transparent pipe (1) above the second thin film (21) and sealing it; and a cotton wool (4) is provided at the lower end of the second plastic transparent pipe (2) for sealing.

2. The artificial breeding device for insects with piercing-sucking mouthparts according to claim 1, characterized in that: Nutrient solution for piercing-sucking insects is provided in the first plastic transparent pipe (1) and between the first thin film (11) and the second thin film (21); the piercing-sucking insects are raised in the second plastic transparent pipe (2) and between the second thin film (21) and the absorbent cotton (4).

3. The artificial breeding device for insects with piercing-sucking mouthparts according to claim 1, characterized in that: The connecting device (3) includes but is not limited to: a liquid injection tube, an adhesive tape, an adhesive strip, an adhesive plaster or a thin film.

4. The artificial breeding device for insects with piercing-sucking mouthparts according to claim 1, characterized in that: The first thin film (11) or the second thin film (21) is a Parafilm sealing film.

5. The artificial breeding device for insects with piercing-sucking mouthparts according to claim 1, characterized in that: The first plastic transparent pipe (1) and the second plastic transparent pipe (2) have the same inner diameter and outer diameter, and the length of the first plastic transparent pipe (1) is shorter than the length of the second plastic transparent pipe (2).

6. The artificial breeding device for insects with piercing-sucking mouthparts according to claim 1, characterized in that: The outer circumferential side of the second plastic transparent pipe (2) is wrapped with black cardboard (5); and an artificial climate light (6) is arranged above the first plastic transparent pipe (1).

7. The artificial breeding device for insects with piercing-sucking mouthparts according to claim 1, characterized in that: A support structure (7) is fixedly arranged at the upper end of the second plastic transparent pipe (2); the support structure (7) is a circular support plate, and a plurality of through holes (8) are arranged on one side surface of the support plate.

8. The artificial breeding device for insects with piercing-sucking mouthparts according to claim 7, characterized in that: The outer diameter of the support plate is the same as the outer diameter of the second plastic transparent pipe (2); the bottom of the support plate is fixedly connected to the top of the second plastic transparent pipe (2); the bottom of the second thin film (21) is in contact with the top of the support plate.

9. The artificial breeding device for insects with piercing-sucking mouthparts according to claim 7, characterized in that: The outer diameter of the support plate is adapted to the inner diameter of the second plastic transparent pipe (2); the outer circumferential side surface of the support plate is fixedly connected to the inner circumferential side surface of the second plastic transparent pipe (2); the upper surface of the support plate and the upper surface of the second plastic transparent pipe (2) are in the same plane; the lower surface of the second thin film (21) is in contact with the upper surface of the support plate.

10. The artificial breeding device for insects with piercing-sucking mouthparts according to claim 7, characterized in that: The thickness of the support plate is less than 100 μm.

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