Quantitative pipetting mechanism and molecular direct diffusion detection device

Abstract

The utility model belongs to the field of molecule detection, and particularly relates to a quantitative pipetting mechanism and a molecule direct diffusion detection device.The quantitative pipetting mechanism is provided with a liquid conveying pipe, a quantitative suction assembly and a driving part, and the quantitative suction assembly comprises a negative pressure forming part and an exhaust pipe communicated with a suction opening of the negative pressure forming part. The end, away from the negative pressure forming piece, of the exhaust pipe is communicated with the top of the reaction pipe, the bottom of the storage pipe is communicated with the reaction pipe through the liquid conveying pipe, then the driving piece is driven to move relative to the carrier, the negative pressure forming piece is triggered to act through the driving piece, and a preset negative pressure value is generated in the reaction pipe. And the liquid transfer amount is controlled by the negative pressure forming piece, so that the operation is simple, errors are not easy to occur, and the accuracy of a subsequent detection result is ensured. The molecular direct diffusion detection device using the quantitative pipetting mechanism is also simple to operate, the liquid transfer amount is not easy to make mistakes, and the accuracy of the detection result is high.

Description

Technical Field

[0001] The present application relates to the technical field of molecular detection, and in particular to a quantitative liquid transfer mechanism and a molecular direct diffusion detection device. Background Art

[0002] The characteristic of molecular POCT (point-of-care testing) of "sample in, result out" not only directly lowers the threshold of nucleic acid testing, breaks the shackles of "four suites" of nucleic acid testing, but also benefits the miniaturization and portability of qPCR testing. The key to realizing molecular POCT is to simplify sample processing. Direct PCR is a technology that can achieve in vitro nucleic acid amplification without the need for additional nucleic acid extraction, eliminating the tedious procedure of extracting genomic DNA, and can be directly applied to rapid PCR testing.

[0003] The current molecular detection experiment process using the direct amplification method is as follows: after collecting a pharyngeal or nasal swab from a patient, it is placed in a cell preservation tube for storage, and then the sample solution is quantitatively drawn by a human or machine and added to a PCR tube that has been filled with PCR reagents, and then the amplification test is performed on the machine. Since the addition of sample solution to the PCR tube is manually controlled by a dropper or a pipette, the amount of addition is difficult to operate and prone to errors, which affects the accuracy of subsequent test results. Utility Model Content

[0004] The embodiments of the present application provide a quantitative pipetting mechanism and a molecular direct amplification detection device, which are used to solve the problem in existing direct amplification methods that the amount of sample liquid transferred to the PCR tube is difficult to control, prone to errors, and affects the accuracy of subsequent detection results.

[0005] To this end, according to one aspect of the present application, a quantitative pipetting mechanism is provided, comprising:

[0006] A carrier having a sample position for fixing a storage tube and a detection position for fixing a reaction tube;

[0007] An infusion tube, used to connect the storage tube and the reaction tube;

[0008] a quantitative suction assembly, comprising a negative pressure forming member and a suction pipe having one end connected to a suction port of the negative pressure forming member, and the other end of the suction pipe being able to be connected to the top of the reaction tube; and

[0009] The driving member is slidably arranged on the carrier. During the movement of the driving member relative to the carrier, the negative pressure forming member can be triggered to generate a preset negative pressure value in the reaction tube, so that the liquid in the storage tube is quantitatively pumped to the reaction tube through the infusion tube.

[0010] Optionally, the negative pressure forming member includes a piston cylinder and a piston slidably disposed in the piston cylinder, the end of the exhaust pipe away from the reaction tube is connected to the interior of the piston cylinder, and the piston is connected to the driving member; during the movement of the driving member relative to the carrier, the piston cylinder can be limited on the carrier so that the piston and the piston cylinder move relative to each other, thereby generating the preset negative pressure value in the reaction tube.

[0011] Optionally, the quantitative pipetting mechanism also includes a puncture assembly, and the end of the infusion tube away from the reaction tube is connected to the puncture assembly, and the movement path of the driving member has at least a starting position, an intermediate position and an end position. During the process of the driving member moving from the starting position to the intermediate position, the driving member can drive the puncture assembly to pierce the preservation tube; during the process of the driving member moving from the intermediate position to the end position, the driving member can trigger the negative pressure forming member and generate the preset negative pressure value in the reaction tube.

[0012] Optionally, the puncture assembly includes a slider slidably disposed on the carrier and a puncture needle mounted on the slider, the lower end of the preservation tube is located on the movement path of the slider, the infusion tube is connected to the puncture needle, the movement path of the slider intersects with the movement path of the driving member, and the slider can be pushed during the movement of the driving member from the starting position to the intermediate position so that the puncture needle penetrates the preservation tube.

[0013] Optionally, the sliding block is slidably disposed on the carrier along a first direction, and the driving member is slidably disposed on the carrier along a second direction, and the second direction is perpendicular to the first direction.

[0014] Optionally, a push slope is provided on the slider, and a top slope is provided on the driving member. When the driving member moves from the starting position to the intermediate position, the top slope pushes the push slope, so that the slider drives the puncture needle to pierce the storage tube.

[0015] Optionally, the quantitative pipetting mechanism further comprises an adapter, the adapter is movably arranged on the driving member, two needles are arranged on the adapter, one end of the infusion tube away from the puncture assembly and one end of the air extraction tube away from the negative pressure forming member are respectively connected to the two needles; on the movement path of the driving member, there is also a front position between the starting position and the middle position;

[0016] During the process of the driving member moving from the starting position to the front position, the driving member drives the adapter to drive the two needles to penetrate the top of the reaction tube;

[0017] During the process of the driving member moving from the starting position to the front position, the driving member gradually approaches and abuts against the slider, and during the process of the driving member moving from the front position to the middle position, the driving member pushes the slider, so that the slider drives the puncture needle to pierce the storage tube;

[0018] During the process of the driving member moving from the front position to the end position, the adapter member is stationary relative to the carrier.

[0019] Optionally, the driving member has two side plates arranged at intervals, and the adapter has two side strips arranged at intervals, the relative outer sides of the two side strips are clamped with the relative inner sides of the two side plates, and a separation structure is provided on the carrier on the movement path of the two side plates. In the process of the driving member moving from the starting position to the front position, the adapter moves with the driving member to drive the two needles to penetrate the top of the reaction tube; in the process of the driving member moving from the front position to the middle position, the separation structure is inserted into the relative inner sides of the two side plates and spreads the two side plates apart to separate the side strips from the side plates.

[0020] According to another aspect of the present application, a molecular direct diffusion detection device is provided, comprising:

[0021] A quantitative pipetting mechanism as described above;

[0022] A storage tube fixed to the sample position, wherein a storage liquid is stored in the storage tube, and a puncture portion that can be pierced is provided at the lower end of the storage tube; and

[0023] A reaction tube is fixed at the detection position, wherein the detection reagent is stored in the reaction tube, and the top of the reaction tube is sealed by a rubber plug.

[0024] Optionally, a support column extending upward from the bottom of the storage tube is provided inside the storage tube, and the height of the support column is greater than the distance between the center of the puncture portion and the bottom of the storage tube.

[0025] The quantitative pipetting mechanism and molecular direct expansion detection device provided by the present application have the beneficial effects that: compared with the prior art, the quantitative pipetting mechanism of the present application is provided with an infusion tube, a quantitative suction assembly and a driving member, and the quantitative suction assembly includes a negative pressure forming member and an exhaust pipe connected to the suction port of the negative pressure forming member. In actual use, the end of the exhaust pipe away from the negative pressure forming member is connected to the top of the reaction tube, and the bottom of the storage tube is connected to the reaction tube by the infusion tube, and then the driving member is driven to move relative to the carrier, and the negative pressure forming member is triggered by the driving member to generate a preset negative pressure value in the reaction tube, so that the liquid in the storage tube is quantitatively pumped to the reaction tube through the infusion tube, and the amount of liquid transferred is controlled by the negative pressure forming member, which is simple to operate and not prone to errors, thereby ensuring the accuracy of subsequent test results. The molecular direct expansion detection device using the quantitative pipetting mechanism is also simple to operate, the amount of liquid transferred is not prone to errors, and the accuracy of the test results is high. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0027] in:

[0028] Figure 1 is a schematic diagram of the external structure of a molecular direct diffusion detection device shown in one embodiment of the present application;

[0029] Figure 2 It is a structural schematic diagram of a quantitative liquid transfer mechanism shown in an embodiment of the present application after the carrier is removed and the driving member is located in the starting position;

[0030] Figure 3 is a schematic cross-sectional structural diagram of a carrier of a quantitative liquid transfer mechanism shown in an embodiment of the present application;

[0031] Figure 4 It is a schematic cross-sectional structure diagram of a quantitative liquid transfer mechanism shown in an embodiment of the present application when the driving member is in a front position;

[0032] Figure 5 It is a schematic cross-sectional structure diagram of a quantitative liquid transfer mechanism shown in an embodiment of the present application when the driving member is located in the middle position;

[0033] Figure 6 is a schematic cross-sectional structural diagram of a quantitative liquid transfer mechanism shown in an embodiment of the present application when the driving member is in a stop position;

[0034] Figure 7It is a schematic diagram of the structure of a quantitative liquid transfer mechanism shown in an embodiment of the present application when the driving member and the adapter are not separated;

[0035] Figure 8 It is a schematic diagram of the structure after the driving component and the adapter component in the quantitative pipetting mechanism are separated according to one embodiment of the present application.

[0036] Description of main component symbols:

[0037] 10. Storage tube; 11. Puncture part; 12. Tube cover; 13. Support column;

[0038] 20. Reaction tube; 21. Rubber stopper;

[0039] 100, carrier; 1001, first insertion port; 1002, second insertion port; 1003, storage slot; 101, slide slot; 110, separation structure;

[0040] 200, puncture assembly; 210, slider; 211, push ramp; 220, puncture needle;

[0041] 300, infusion tube;

[0042] 400, driving member; 401, top-butting inclined surface; 420, side plate; 421, card slot;

[0043] 500, putter;

[0044] 600, negative pressure forming member; 610, piston cylinder; 611, guide rib; 620, piston;

[0045] 700, exhaust pipe;

[0046] 800, piston rod;

[0047] 900, adapter; 910, side strip; 911, clamping protrusion; 920, connecting block;

[0048] 1000. Needle. DETAILED DESCRIPTION

[0049] In order to facilitate the understanding of the present application, the present application will be described more comprehensively with reference to the relevant drawings below. The preferred embodiments of the present application are provided in the drawings. However, the present application can be implemented in many other different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present application more thorough and comprehensive.

[0050] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0051] It should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0052] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features.

[0053] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.

[0054] It should also be noted that, in the embodiments of the present application, the same figure mark is used to represent the same component or the same part. For the same parts in the embodiments of the present application, the figure may only mark one of the parts or components as an example. It should be understood that the figure mark also applies to other identical parts or components.

[0055] As described in the background technology, in existing molecular detection using direct amplification methods, the addition of sample liquid into the PCR tube is manually controlled by a dropper or a pipette. The amount of addition is difficult to operate and prone to errors, which affects the accuracy of subsequent test results.

[0056] In order to solve the above problems, according to one aspect of the present application, an embodiment of the present application provides a quantitative liquid transfer mechanism, such as Figure 5-Figure 6As shown, the quantitative liquid transfer mechanism includes a carrier 100, a liquid infusion tube 300, a quantitative suction assembly, and a driving member 400. The carrier 100 has a sample position for fixing the storage tube 10 and a detection position for fixing the reaction tube 20. The liquid infusion tube 300 is used to connect the storage tube 10 and the reaction tube 20. The quantitative suction assembly includes a negative pressure forming member 600 and an air suction pipe 700 whose one end is connected to the suction port of the negative pressure forming member 600, and the other end of the air suction pipe 700 can be connected to the top of the reaction tube 20. The driving member 400 is slidably arranged on the carrier 100. When the driving member 400 moves relative to the carrier 100, it can trigger the negative pressure forming member 600 and generate a preset negative pressure value in the reaction tube 20, so that the liquid in the storage tube 10 is quantitatively pumped to the reaction tube 20 through the liquid infusion tube 300.

[0057] In the embodiment of the present application, when the quantitative pipetting mechanism is actually used, the end of the vacuum tube 700 away from the negative pressure forming member 600 is connected to the top of the reaction tube 20, and the bottom of the storage tube 10 is connected to the reaction tube 20 by using the infusion tube 300, and then the driving member 400 is driven to move relative to the carrier 100. The negative pressure forming member 600 is triggered by the driving member 400 to generate a preset negative pressure value in the reaction tube 20, and then the liquid in the storage tube 10 is quantitatively pumped to the reaction tube 20 through the infusion tube 300. The amount of liquid transferred is controlled by the negative pressure forming member 600, which is simple to operate and not prone to errors, thereby ensuring the accuracy of subsequent test results.

[0058] In one embodiment, if Figure 5-Figure 6 As shown, the negative pressure forming member 600 includes a piston cylinder 610 and a piston 620 slidably disposed in the piston cylinder 610, the end of the exhaust pipe 700 away from the reaction tube 20 is connected to the interior of the piston cylinder 610, and the piston 620 is connected to the driving member 400; during the movement of the driving member 400 relative to the carrier 100, the piston cylinder 610 can be limited on the carrier 100, so that the piston 620 moves relative to the piston cylinder 610 under the drive of the driving member 400, thereby generating a preset negative pressure value in the reaction tube 20.

[0059] By the above arrangement, the piston 620 is moved in the piston cylinder 610 to draw the gas in the reaction tube 20 into the piston cylinder 610 through the exhaust pipe 700. The structure is simple, wherein the size of the preset negative pressure value, that is, the amount of transferred liquid is determined by the distance the piston 620 moves relative to the piston cylinder 610, which is simple and reliable.

[0060] Specifically, a piston rod 800 is fixed to the driving member 400 , and the driving member 400 is connected to the piston 620 via the piston rod 800 extending into the piston cylinder 610 .

[0061] In another embodiment, not shown in the figure, the negative pressure forming member includes a piston cylinder and a piston slidably arranged in the piston cylinder, and the exhaust pipe is connected to the interior of the piston cylinder. Different from the above embodiment, the piston cylinder is connected to the driving member, and during the movement of the driving member relative to the carrier, the piston can be limited on the carrier to make the piston cylinder and the piston move relative to each other.

[0062] In one embodiment, if Figure 2 and Figure 5-Figure 6 As shown, the quantitative pipetting mechanism also includes a puncture assembly 200, and the end of the infusion tube 300 away from the reaction tube 20 is connected to the puncture assembly 200, and the storage tube 10 has a puncture portion 11 that can be punctured (illustratively, the lower end of the storage tube 10 is provided with a through hole, and the through hole is blocked by a rubber cap, and the rubber cap forms the puncture portion 11). There is at least a starting position ( Figure 2 The position shown in ), the middle position ( Figure 5 ) and the end position ( Figure 6 In the process of the driving member 400 moving from the starting position to the middle position, the driving member 400 can drive the puncture assembly 200 to pierce the storage tube 10; in the process of the driving member 400 moving from the middle position to the end position, the driving member 400 can trigger the negative pressure forming member 600 and generate a preset negative pressure value in the reaction tube 20.

[0063] Specifically, the puncture assembly 200 includes a slider 210 slidably disposed on the carrier 100 and a puncture needle 220 installed on the slider 210. The lower end of the preservation tube 10 is located on the movement path of the slider 210. The infusion tube 300 is connected to the puncture needle 220. The movement path of the slider 210 intersects with the movement path of the driving member 400. When the driving member 400 moves from the starting position to the middle position, the slider 210 can be pushed so that the puncture needle 220 can penetrate the preservation tube 10.

[0064] Preferably, the slider 210 is slidably disposed on the carrier 100 along a first direction, and the driving member 400 is slidably disposed on the carrier 100 along a second direction, and the second direction is perpendicular to the first direction.

[0065] The sliding directions of the sliding member and the driving member 400 are perpendicular, which facilitates the determination of the movement tracks and strokes of the sliding member and the driving member 400. For example, the first direction is the horizontal direction in the figure, and the second direction is the vertical direction in the figure. At the same time, the second direction is also the height direction of the storage tube 10 and the reaction tube 20. This arrangement facilitates the application of force to the driving member 400, and when in use, it is only necessary to press the driving member 400 up and down.

[0066] In a specific embodiment, see Figure 2As shown, the slider 210 is provided with a push inclined surface 211, and the driving member 400 is provided with a top inclined surface 401. The driving member 400 is moved from the starting position ( Figure 2 ) to the middle position ( Figure 5 During the process of moving the slider 210 to the position shown in FIG1 , the push inclined surface 211 is pushed by the push inclined surface 401, so that the slider 210 drives the puncture needle 220 to pierce the storage tube 10. The push inclined surface 211 has the same inclination as the push inclined surface 401, and thus the contact area is increased and the stability of the slider 210 during the movement is improved.

[0067] In a specific embodiment, Figure 2 and Figure 4-Figure 6 As shown, the quantitative liquid transfer mechanism also includes an adapter 900, which is movably arranged on the driving member 400, and two needles 1000 are arranged on the adapter 900, and the end of the infusion tube 300 away from the puncture assembly 200 and the end of the suction tube 700 away from the negative pressure forming member 600 are respectively connected to the two needles 1000; on the movement path of the driving member 400, there is a front position between the starting position and the middle position; in the process of the driving member 400 moving from the starting position to the front position, the driving member 400 drives The dynamic adapter 900 drives the two needles 1000 to pierce the top of the reaction tube 20; when the driving member 400 moves from the starting position to the front position, the driving member 400 gradually approaches and abuts against the slider 210, and when the driving member 400 moves from the front position to the middle position, the driving member 400 pushes the slider 210 so that the slider 210 drives the puncture needle 220 to pierce the storage tube 10; when the driving member 400 moves from the front position to the end position, the adapter 900 is stationary relative to the carrier 100.

[0068] As configured above, after the action of pressing the driving member 400, the following stages are sequentially experienced:

[0069] When the driving member 400 is at the starting position ( Figure 2 ) to the front position ( Figure 4 During the process of moving the reaction tube 20 to the position shown in FIG. 1 ), the driving member 400 drives the adapter 900 to move together, and the needle 1000 connected to the infusion tube 300 and the needle 1000 connected to the exhaust tube 700 penetrate into the top of the reaction tube 20, so that the reaction tube 20 is connected to the outside through the infusion tube 300, and the air pressure in the reaction tube 20 is balanced, so that the air pressure in the reaction tube 20 is the same as the atmospheric pressure;

[0070] When the driving member 400 is moved from the front position ( Figure 4 ) to the middle position ( Figure 5During the process of moving the slider 210 to the position shown in FIG. 1 ), the driving member 400 pushes the slider 210, so that the slider 210 drives the puncture needle 220 to penetrate the storage tube 10, so that the bottom of the storage tube 10 and the top of the reaction tube 20 are connected through the infusion tube 300. Meanwhile, during this process, the adapter 900 is separated from the driving member 400 and remains stationary relative to the carrier 100.

[0071] When the driving member 400 is at the starting position ( Figure 2 ) to the middle position ( Figure 5 During the process of the driving member 400 moving from the middle position (the position shown in FIG), the driving member 400 drives the piston 620 and the piston cylinder 610 to move together, that is, the negative pressure forming member 600 is not triggered. Figure 5 ) to the end position ( Figure 6 During the process of moving the piston cylinder 610 to the position shown in the figure, the piston cylinder 610 is limited by the stopper on the carrier 100 and is relatively stationary with respect to the carrier 100, and the driving member 400 drives the piston 620 to move in the piston cylinder 610, and the gas in the reaction tube 20 is sucked through the suction pipe 700, so that a preset negative pressure value is generated in the reaction tube 20, and then the liquid in the preservation tube 10 is quantitatively pumped to the reaction tube 20 through the infusion tube 300.

[0072] In one implementation, the carrier 100 is provided with a slide groove 101 extending along the movement direction of the driving member 400, the length of the slide groove 101 is the same as the movement distance of the driving member 400 from the starting position to the middle position, and the outer wall of the piston cylinder 610 is provided with a guide rib 611, and the guide rib 611 is slidably arranged in the slide groove 101. In the process of the driving member 400 moving from the starting position to the middle position, the guide rib 611 slides in the slide groove 101, and the two cooperate to guide the movement of the piston cylinder 610; in the middle position, the guide rib 611 abuts against the lower end groove wall of the slide groove 101, and the groove wall of the slide groove 101 here forms a stopper, which limits the piston cylinder 610 from continuing to move downward with the driving member 400, so that the piston cylinder 610 is stationary relative to the carrier 100 in the process of the driving member 400 moving from the middle position to the end position.

[0073] In a more specific embodiment, Figure 7 and Figure 8As shown, the driving member 400 has two spaced-apart side plates 420, the adapter 900 includes two spaced-apart side strips 910 and a connecting block 920 connected between one end of the two side strips 910, two needles 1000 are arranged on the connecting block 920, and the relative outer sides of the two side strips 910 are engaged with the relative inner sides of the two side plates 420 (specifically, the relative inner sides of the two side plates 420 are provided with a card slot 421, and the relative outer sides of the other ends of the two side strips 910 are provided with a card protrusion 911, and the adapter 900 is connected through the card protrusion 911 at the ends of the two side strips 910 1 is stuck in the relatively inner slots 421 of the two side plates 420), and a separation structure 110 is provided on the carrier 100 on the movement path of the two side plates 420. When the driving member 400 moves from the starting position to the front position, the adapter 900 moves together with the driving member 400 to drive the two needles 1000 to pierce the top of the reaction tube 20; when the driving member 400 moves from the front position to the middle position, the separation structure 110 is inserted into the relatively inner sides of the two side plates 420 and the two side plates 420 are opened to separate the side strips 910 from the side plates 420.

[0074] The separation structure 110 is composed of two separation sheets. When in the front position, the two separation sheets are inserted into the opposite inner sides of the two side plates 420. As the driving member 400 continues to move from the front position to the end position, the two separation sheets open the two side plates 420 to separate the protrusion 911 at the end of the side strip 910 from the groove 421 in the side plate 420, so that the adapter 900 is stationary relative to the carrier 100.

[0075] In a specific embodiment, Figure 1 and Figure 3-Figure 6 As shown, the carrier 100 is a shell-like structure, and the sample position, detection position, quantitative suction assembly, puncture assembly 200, infusion tube 300 and driving member 400 are all arranged in the shell-like carrier 100, and the carrier 100 is used to protect the quantitative suction assembly, puncture assembly 200, infusion tube 300 and driving member 400. The top of the shell-like carrier 100 is also provided with a first insertion port 1001 for inserting the storage tube 10 into the sample position, and the bottom of the shell-like carrier 100 is provided with a second insertion port 1002 for inserting the reaction tube 20 into the detection position.

[0076] Furthermore, in order to facilitate the application of external force to the driving member 400, a push rod 500 is hinged on the driving member 400, and a storage groove 1003 for accommodating the push rod 500 is also provided on the carrier 100. Before use, the push rod 500 can be flipped relative to the driving member 400 and stored in the storage groove 1003. When in use, the push rod 500 is flipped out of the storage groove 1003 and the push rod 500 is pressed.

[0077] According to another aspect of the present application, the embodiments of the present application also provide a molecular direct diffusion detection device, such as Figure 1-Figure 6As shown, the molecular direct amplification detection device comprises the quantitative pipetting mechanism in any of the above embodiments, a storage tube 10 and a reaction tube 20, wherein the storage tube 10 is fixed at the sample position, the storage tube 10 stores a storage solution, and the lower end of the storage tube 10 has a puncture portion 11 that can be pierced. The reaction tube 20 is fixed at the detection position, the reaction tube 20 stores a detection reagent, and the top of the reaction tube 20 is sealed by a rubber plug 21.

[0078] Since the molecular direct amplification detection device adopts the quantitative pipetting mechanism in the above-mentioned embodiment, during the process of transferring liquid between the storage tube 10 and the reaction tube 20, there is no need to open the cover, aspirate the liquid with a pipette needle / dropper, pipette and close the cover, and quantitative pipetting can be achieved by pressing the driving member 400. The operation is simple and easy to use, which ensures the accuracy of subsequent detection results and reduces the risk of errors and biological exposure contamination.

[0079] In one implementation, Figure 4-Figure 6 As shown, a support column 13 extending upward from the bottom of the storage tube 10 is provided inside the storage tube 10 , and the height of the support column 13 is greater than the distance between the center of the puncture portion 11 and the bottom of the storage tube 10 .

[0080] The support column 13 is provided to support the sampling test piece that is broken into the storage tube 10 , so as to prevent the puncture needle 220 from piercing into the sampling test piece and clogging the puncture needle 220 .

[0081] In summary, in one example, the storage tube 10 is a sampling tube, in which a cell preservation solution is stored, and the reaction tube 20 is a PCR tube, in which paraffin balls and PCR reagents are stored. The entire use process of the molecular direct amplification detection device is as follows:

[0082] 1. Open the tube cover 12 of the sampling tube, break the sampling test piece into the tube, and tighten the tube cover 12;

[0083] 2. If Figure 1 As shown, flip up the push rod 500;

[0084] 3. Figure 2 and Figure 4 As shown, by pressing the push rod 500, the driving member 400 drives the two needles 1000 on the adapter 900 above the PCR tube to pierce the rubber plug 21 of the PCR tube;

[0085] 4. Figure 5 As shown, the push rod 500 is continuously pressed, the adapter 900 above the PCR tube is separated from the driving member 400, and the driving member 400 drives the puncture needle 220 on the slider 210 on the side of the sample liquid tube to puncture the puncture portion 11 at the lower end of the sampling tube;

[0086] 5. If Figure 6As shown, continue to press the push rod 500. After the piston cylinder 610 reaches the limited position, the piston 620 moves downward under the drive of the driving member 400 to start extracting the gas in the PCR tube, and quantitatively extracts the liquid containing the sample in the tube into the PCR tube to reconstitute the reagent freeze-dried ball.

[0087] The technical features of the above embodiments may be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0088] The above embodiments only express several implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present application, and these all belong to the protection scope of the present application. Therefore, the protection scope of the present application shall be subject to the attached claims.

Claims

1. A quantitative liquid transfer mechanism, characterized in that: include: A carrier (100) having a sample position for fixing a storage tube (10) and a detection position for fixing a reaction tube (20); A liquid infusion tube (300), used for connecting the storage tube (10) and the reaction tube (20); A quantitative suction component, comprising a negative pressure forming member (600) and a suction pipe (700) having one end connected to a suction port of the negative pressure forming member (600), and the other end of the suction pipe (700) can be connected to the top of the reaction tube (20); as well as The driving member (400) is slidably disposed on the carrier (100), and can trigger the negative pressure forming member (600) and generate a preset negative pressure value in the reaction tube (20) during the movement of the driving member (400) relative to the carrier (100), so that the liquid in the storage tube (10) is quantitatively pumped into the reaction tube (20) through the infusion tube (300).

2. The quantitative liquid transfer mechanism according to claim 1, characterized in that: The negative pressure forming member (600) includes a piston cylinder (610) and a piston (620) slidably disposed in the piston cylinder (610); one end of the exhaust pipe (700) away from the reaction tube (20) is connected to the interior of the piston cylinder (610); the piston (620) is connected to the driving member (400); during the movement of the driving member (400) relative to the carrier (100), the piston cylinder (610) can be limited on the carrier (100) so that the piston (620) and the piston cylinder (610) move relative to each other, thereby generating the preset negative pressure value in the reaction tube (20).

3. The quantitative liquid transfer mechanism according to claim 1 or 2, characterized in that: The quantitative liquid transfer mechanism further comprises a puncture assembly (200), one end of the infusion tube (300) away from the reaction tube (20) is connected to the puncture assembly (200), and the movement path of the driving member (400) has at least a starting position, an intermediate position and an end position. When the driving member (400) moves from the starting position to the intermediate position, the driving member (400) can drive the puncture assembly (200) to puncture the storage tube (10); when the driving member (400) moves from the intermediate position to the end position, the driving member (400) can trigger the negative pressure forming member (600) and generate the preset negative pressure value in the reaction tube (20).

4. The quantitative liquid transfer mechanism according to claim 3, characterized in that: The puncture assembly (200) comprises a slider (210) slidably arranged on the carrier (100) and a puncture needle (220) mounted on the slider (210); the lower end of the storage tube (10) is located on the movement path of the slider (210); the infusion tube (300) is connected to the puncture needle (220); the movement path of the slider (210) intersects with the movement path of the driving member (400); when the driving member (400) moves from the starting position to the intermediate position, the slider (210) can be pushed so that the puncture needle (220) pierces the storage tube (10).

5. The quantitative liquid transfer mechanism according to claim 4, characterized in that: The sliding block (210) is slidably disposed on the carrier (100) along a first direction, and the driving member (400) is slidably disposed on the carrier (100) along a second direction, wherein the second direction is perpendicular to the first direction.

6. The quantitative liquid transfer mechanism according to claim 4, characterized in that: The slider (210) is provided with a push inclined surface (211), and the driving member (400) is provided with a top push inclined surface (401). When the driving member (400) moves from the starting position to the intermediate position, the top push inclined surface (401) pushes the push inclined surface (211), so that the slider (210) drives the puncture needle (220) to pierce the storage tube (10).

7. The quantitative liquid transfer mechanism according to claim 4, characterized in that: The quantitative liquid transfer mechanism further comprises a transfer member (900), the transfer member (900) being movably arranged on the driving member (400), the transfer member (900) being provided with two needles (1000), one end of the infusion tube (300) away from the puncture assembly (200) and one end of the air extraction tube (700) away from the negative pressure forming member (600) being respectively connected to the two needles (1000); on the movement path of the driving member (400), there is also a front position between the starting position and the intermediate position; During the process of the driving member (400) moving from the starting position to the front position, the driving member (400) drives the adapter (900) to drive the two needles (1000) to penetrate the top of the reaction tube (20); During the process of the driving member (400) moving from the starting position to the front position, the driving member (400) gradually approaches and abuts against the slider (210); during the process of the driving member (400) moving from the front position to the middle position, the driving member (400) pushes the slider (210) so that the slider (210) drives the puncture needle (220) to pierce the storage tube (10); During the process of the driving member (400) moving from the front position to the end position, the adapter (900) remains stationary relative to the carrier (100).

8. The quantitative liquid transfer mechanism according to claim 7, characterized in that: The driving member (400) is provided with two side plates (420) arranged at intervals, and the adapter (900) is provided with two side strips (910) arranged at intervals, and the relative outer sides of the two side strips (910) are clamped with the relative inner sides of the two side plates (420). A separation structure (110) is provided on the carrier (100) on the movement path of the two side plates (420). When the driving member (400) moves from the starting position to the front position, the adapter (900) moves together with the driving member (400) to drive the two needles (1000) to penetrate the top of the reaction tube (20); when the driving member (400) moves from the front position to the middle position, the separation structure (110) is inserted into the relative inner sides of the two side plates (420) and spreads the two side plates (420) apart to separate the side strips (910) from the side plates (420).

9. A molecular direct diffusion detection device, characterized in that: include: The quantitative pipetting mechanism according to any one of claims 1 to 8; A storage tube (10) is fixed to the sample position, wherein a storage liquid is stored in the storage tube (10), and the lower end of the storage tube (10) has a puncture portion (11) that can be pierced; and A reaction tube (20) is fixed at the detection position. The reaction tube (20) stores a detection reagent. The top of the reaction tube (20) is sealed by a rubber plug (21).

10. The molecular direct diffusion detection device according to claim 9, characterized in that: A support column (13) extending upward from the bottom of the storage tube (10) is provided inside the storage tube (10), and the height of the support column (13) is greater than the distance between the center of the puncture portion (11) and the bottom of the storage tube (10).

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