Light path device of microplate reader

Abstract

The utility model discloses an optical path device of a microplate reader, which solves the problems of poor shading property, unstable experimental data and easy aging of an optical filter mounting device in the traditional microplate reader. The light path device adjusts the position of a light filter fixing wheel through rotation of a power device. The optical filter fixing wheel is connected with the fixing frame through a middle shaft of the fixing frame, and different optical filters are fixed on the other side of the optical filter fixing wheel. A light source of the light path device emits light beams, the light beams with different wavelengths are obtained through a light filter on a light filter fixing wheel, and light signals are converted into corresponding electric signals through an optical fiber connector and then enter a detection system. According to the utility model, the service life can be prolonged, light beams can be stabilized, and experimental data are ensured to be stable and reliable.

Description

Technical Field

[0001] The utility model relates to the field of enzyme markers, in particular to an optical path device of an enzyme marker. Background Art

[0002] At present, the installation device of the filter is widely used in instruments such as fluorescence quantitative PCR instrument and microplate reader, which can match filters of multiple bands. Different filters meet the experimental requirements of different bands and have different performance effects and requirements in different working environments. At present, most of the filters on the market are made of plastic products and machined. Under ultraviolet irradiation, aging and deformation will occur, resulting in reduced stability and accuracy in the later stage, and increasing the instability of experimental results.

[0003] The rotating wheel device is made of plastic material, and the filter is fixed by the thread of the plastic part itself. The advantages of this method are easy and quick installation and low manufacturing cost. The disadvantages are that it is easy to accelerate aging in the working environment, causing deformation, and it is easy to damage the filter. It is very easy to become loose during transportation, causing the filter to move, and the other positions without filters cannot achieve good light shielding.

[0004] The method uses machining and sheet metal with a single-piece fixed structure for one-to-one use. The advantage of this method is that it can effectively ensure the stability and accuracy of a single optical path and has a good anti-aging effect. The disadvantage is that it is inconvenient to use. Each time a different filter is replaced, other tooling is required to manually adjust the position of the limit, which brings great uncertainty to the user and affects the test results. Utility Model Content

[0005] In order to solve the problems of poor light shielding, unstable experimental data and easy aging of filter installation devices in traditional microplate readers, the utility model provides an optical path device suitable for the field of microplate readers to solve such problems, adopts a simple and efficient design concept, and ensures the reliability and consistency of the test results.

[0006] The utility model designs an optical path device of an enzyme labeling instrument, which adopts a rotatable multi-porous filter fixing frame, which can not only meet the function of conveniently installing the filter, but also match filters of multiple bands, and different filters meet the experimental requirements of different bands; a gasket is installed between the filter and the fixing frame, which optimizes the light-shielding problem of the optical path device and makes the experimental data more stable; and the problem of easy aging of the device is improved by changing the material of the device.

[0007] The utility model discloses an optical path device of an enzyme labeling instrument, comprising: a power device and a position sensor for adjusting the position of a corresponding filter in a filter fixing wheel, the filter fixing wheel is fixed on a fixing frame with a central axis so that the fixing wheel can rotate; at the same time, the fixing frame is connected to the power device; a light source is fixed to the light source frame through a light source fixing cover, an emitted light beam is formed into a light beam through a light source beam collector, and is connected to the filter fixing wheel, and after being fixed, the entire device is assembled on a bottom plate.

[0008] The technical solution is as follows: In this utility model solution, a light beam excited from the light source 114 passes through a light source beam buncher, and is blocked and gathered by a shading baffle to concentrate the light source and reduce light scattering, and the required light beam is hit on the filter. Through the multi-hole corresponding filter on the filter fixing wheel, the wavelength of the light beam is changed according to the different refractive indices of different filters. A specific filter can be used to obtain a light beam of a specified wavelength for detection, and the changed light beam is converted into a photoelectric signal through the action of light in the optical fiber connector.

[0009] Beneficial effects: The cooperation between the power device and the filter fixing wheel can effectively control different wavelength filters for experiments. After being installed into a module, it can effectively maintain the consistency of the optical path, the accuracy of filter switching, the horizontality of the optical path, and ensure the consistency of parameters between each device, thereby improving the stability in the experiment.

[0010] On the basis of the above technical solution, the present invention can also make the following improvements.

[0011] Furthermore, the filter wheel moving part in the optical path device includes a position sensor, and the position sensor is located on the fixing frame, and the position result is determined by the position sensor.

[0012] The beneficial effect of adopting the above further solution is that the corresponding position sensor can effectively ensure the consistency of each filter and the optical path, thereby improving the accuracy of the experiment.

[0013] Furthermore, the filter wheel assembly in the optical path device comprises a gasket, and the gasket is located between the filter and the fixing plate for filling transition.

[0014] The beneficial effect of adopting the above further solution is that the gasket can effectively achieve a sealing effect and prevent the filter from having direct contact with the fixed plate, thereby protecting the filter.

[0015] Furthermore, the light source, the light source frame and the light source fixing cover are connected.

[0016] The beneficial effect of adopting the above further solution is: strengthening the fixation of the light source and ensuring the stability of the light path.

[0017] Furthermore, the filter wheel assembly in the optical path device includes a filter fixing wheel, the filter fixing wheel has 4 to 8 holes, each hole has a different corresponding number, and each fixing hole is spaced the same distance apart and is distributed in a circle around the fixing axis.

[0018] The beneficial effect of adopting the above further solution is that different numbers can correspond to different filters, so that multiple groups of comparative experiments can be carried out simultaneously and the position of the required filter can be found more quickly and conveniently.

[0019] Furthermore, the position sensor cooperates with the power device to adjust the position of the filter fixing wheel.

[0020] The beneficial effect of adopting the above further solution is that while effectively ensuring the consistency of the filter and the optical path, different filter positions can be rotated and replaced to conduct comparative experiments to reduce experimental errors.

[0021] Furthermore, a light shielding plate is provided between the filter and the light source buncher.

[0022] The beneficial effect of adopting the above further solution is that the light shielding plate can concentrate the light beam more, converge the light path more, reduce the scattering of light, and reduce the error of experimental data.

[0023] Furthermore, the filter fixing wheel and the fixing frame of the optical path device are distributed in an axial shape.

[0024] The beneficial effects of adopting the above further scheme are: the axial distribution can directly connect the optical path to ensure the stability of the optical path; it is easy to install and can be directly installed in the effective position, reducing the tedious operation of adjusting the position, thereby effectively ensuring the reliability and consistency of the detection results.

[0025] Furthermore, the filter fixing wheel and the fixing frame of the optical path device are made of metal, and the surface is coated with an oxidation coating.

[0026] The beneficial effect of adopting the above further scheme is that the filter fixing wheel and the fixing frame are made of metal materials. By using the oxidation and blackening technology of metal materials, the diffuse scattering of light during transmission is reduced, the instability caused by the aging of the material itself is reduced, and the stability of the optical path is ensured.

[0027] Furthermore, the optical fiber of the optical path device is fixed on one side of the power device.

[0028] The beneficial effect of adopting the above further solution is that the optical fiber is fixed by the optical fiber bracket, so that the optical fiber output is more stable, and it is on the same axis as the overall device, so that the experimental data is more stable.

[0029] The advantages of the utility model are: by using a rotatable filter fixing frame, while conveniently installing the filter, filters of multiple bands can also be matched, and different filters meet the experimental requirements of different bands; the light shielding problem of the optical path device is optimized, making the experimental data more stable; and the problem of easy aging of the device is improved.

[0030] Additional aspects and advantages of the present invention will be partly given in the following detailed description and partly become apparent from the following description. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 The utility model is a three-dimensional structural exploded diagram of the optical path device of the microplate reader.

[0032] Figure 2 The utility model is a partial schematic diagram of the optical fiber part of the optical path device of the microplate reader.

[0033] Figure 3 The utility model is a partial schematic diagram of a filter fixing wheel of an optical path device of an enzyme labeling instrument.

[0034] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0035] Power device 101, optical fiber connector 102, optical fiber bracket 103, fixing frame 104, position sensor 105, positioner 106, filter fixing wheel 107, filter 108, gasket 109, light shielding baffle 110, light source buncher 112, light source frame 113, light source 114, light source fixing cover 115, optical fiber 116, bottom plate 117, fixing hole 301, fixing shaft 302. DETAILED DESCRIPTION

[0036] The embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present application, and cannot be understood as limiting the present application.

[0037] In the description of the present invention, it should be understood that the descriptions involving orientations, such as up, down, left, right, front, back, etc., or positional relationships are based on the orientations or positional relationships described in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0038] like Figure 1As shown, in the first aspect of the utility model, an optical path device of an ELISA instrument is proposed, and its working method is: a light beam is excited from a light source 114 and passes through a light source bundler 112, and is blocked and converged by a light shielding plate 110 to concentrate the light source and reduce light scattering, and the required light beam is hit on a filter 108, and the wavelength of the light beam is changed according to the different refractive indices of different filters 108 through the porous corresponding filters 108 on the filter fixing wheel 107, and a specific filter 108 can be used to obtain a light beam of a specified wavelength for detection, and the changed light beam is converted into a photoelectric signal with the optical fiber 116 in the optical fiber connector 102 to obtain electrical signals of different strengths, and the electrical signals are processed to obtain corresponding experimental data, and the structure is simple and the detection efficiency is high. An optical path device of an ELISA instrument, the optical path device comprising a filter fixing wheel 107 and a fixing frame 104; the filter fixing wheel 107 is rotatably connected to the fixing frame 104, and a plurality of filters 108 are fixed on the surface of the filter fixing wheel 107; the back of the fixing frame 104 is connected to a power device 101. The fixing frame 104 is connected to a position sensor 105 and a positioner 106. A gasket 109 with its own thickness is connected between the filter 108 and the filter fixing wheel 107. The front of the filter 108 is connected to a light source clustering device 112; the light source clustering device 112 is arranged on a light source frame 113, and a light source 114 is fixed in the optical path system through a light source fixing cover 115, and each fixing hole 301 of the filter fixing wheel 107 is spaced at the same interval. A light shielding baffle 110 is arranged between the filter 108 and the light source clustering device 112. The filter fixing wheel 107 and the fixing frame 104 are located at the same horizontal line. The filter fixing wheel 107 and the fixing frame 104 included in the optical path system are made of metal, and the surface is coated with an oxidation coating.

[0039] The utility model discloses an optical path device of an enzyme labeling instrument, comprising: a power device 101 and a position sensor 105 for adjusting the position of a corresponding filter 108 in a filter fixing wheel 107, the filter fixing wheel 107 being fixed on a fixing frame 104 having a central axis so that the fixing wheel can rotate; at the same time, the fixing frame 104 is connected to the power device 101; a light source 114 is fixed to a light source frame 113 through a light source fixing cover 115, an emitted light beam is formed into a light beam through a light source beam collector 112, and is connected to the filter fixing wheel 107, and after being fixed, the entire device is assembled on a bottom plate 117; a combination of an optical fiber 116, an optical fiber connector 102 and an optical fiber bracket 103 can better protect the optical fiber, and the optical fiber bracket is connected to the power device, which can effectively ensure the consistency of each filter and the optical path, thereby improving the accuracy of the experiment.

[0040] like Figure 1As shown, in the first aspect of the utility model, an optical path device of an ELISA instrument is proposed, and its working method is: a light beam is excited from a light source 114 and passes through a light source bundler 112, and is blocked and gathered by a light shielding baffle 110 to form a concentrated light beam, thereby reducing light scattering, and the required light beam is hit on a filter 108, and the wavelength of the light beam is changed through the multi-hole corresponding filter 108 on a filter fixing wheel 107 according to the different refractive indices of different filters 108. A specific filter 108 can be used to obtain a light beam of a specified wavelength for detection, and the changed light beam is converted into a photoelectric signal with the optical fiber 116 in the optical fiber connector 102 to obtain electrical signals of different strengths, and the electrical signals are processed to obtain corresponding experimental data, and the structure is simple and the detection efficiency is high.

[0041] The utility model discloses an optical path device of an enzyme labeling instrument, comprising: a power device 101 and a position sensor 105 for adjusting the position of a corresponding filter 108 in a filter fixing wheel 107, the filter fixing wheel 107 being fixed on a fixing frame 104 having a central axis so that the fixing wheel can rotate; at the same time, the fixing frame 104 is connected to the power device 101; a light source 114 is fixed to a light source frame 113 through a light source fixing cover 115, an emitted light beam is formed into a light beam through a light source beam collector 112, and is connected to the filter fixing wheel 107, and after being fixed, the entire device is assembled on a bottom plate 117; a combination of an optical fiber 116, an optical fiber connector 102 and an optical fiber bracket 103 can better protect the optical fiber, and the optical fiber bracket is connected to the power device, which can effectively ensure the consistency of each filter and the optical path, thereby improving the accuracy of the experiment.

[0042] In a specific embodiment of the utility model, Figure 1 The position sensor 105 is located on the fixing frame 104 and Figure 1 The first component from left to right is the power device 101, which works together with the position sensor 105 to adjust the filter fixing wheel so that the light beam emitted by the same light source can accurately hit different filters. It can also control the conversion between filters and switch filters with different serial numbers to obtain the corresponding data.

[0043] In a specific embodiment of the utility model, Figure 1 The position sensor 105 is located on the fixing frame 104 and Figure 1 The first component from left to right is the power device 101, which works together with the position sensor 105 to adjust the filter fixing wheel so that the light beam emitted by the same light source can accurately hit different filters. It can also control the conversion between filters and switch between filters with different serial numbers to obtain the data of the corresponding serial number model filter.

[0044] Furthermore, the power device 101 cooperates with the position sensor 105 to quickly find the filter at the required position for conducting experiments according to the experimental requirements, thereby improving the experimental efficiency.

[0045] In a specific embodiment of the utility model, Figure 1 The gasket 109 is located between the filter 108 and the fixed plate. The gasket itself has a certain thickness and can separate the filter 108 from the fixed plate, so that the filter does not need to directly contact the fixed plate, which protects the filter. At the same time, the gasket 109 also has the function of overfilling, which can effectively achieve a sealed effect, making the experimental data more stable and reliable.

[0046] like Figure 1 The shape of the gasket 109 shown is similar to an "O" ring and is located between the filter 108 and the fixed plate. The gasket itself has a certain thickness and can separate the filter 108 from the fixed plate, so that the filter does not need to directly contact the fixed plate, which plays a protective role for the filter. At the same time, the gasket 109 also has the function of overfilling, which can effectively achieve a sealing effect, making the experimental data more stable and reliable.

[0047] In a specific embodiment of the utility model, Figure 1 The light source 114 shown on the right is located between the light source frame 113 and the light source fixing cover 115. The light source is fixed front and back so that the light beam emitted by the light source is sufficiently stable and easy to install. It only needs to connect the light source frame 113, the light source 114 and the light source fixing cover 115 in sequence to align the light beam of the light source without the need for subsequent manual aiming, thereby ensuring the stability and reliability of the experimental data.

[0048] In a specific embodiment of the utility model, Figure 3 The portion shown is a partial schematic diagram of the filter fixing wheel 107, which is marked with the serial number of the optical path channel. It is convenient to distinguish the data for comparative experiments when placing filters with different refractive indices, and it is more convenient to find the position of the required filter, saving time.

[0049] In a specific embodiment of the utility model, Figure 3 The shown part is a partial schematic diagram of the filter fixing wheel 107. The intervals between each fixing hole 301 are the same and they are distributed in a circle around the fixed axis. The regular distribution is conducive to the accuracy when the power device is adjusted to correspond to different shading sheets. The circular distribution is more suitable for a rotating fixing plate, which is convenient and quick to replace the filter for experiments.

[0050] In a specific embodiment of the utility model, Figure 1There is a light shielding plate 110 between the filter 108 and the light source beam collector 112. The light shielding plate converges the light beam emitted by the light source, reduces the scattering of light, makes the light beam more concentrated, and the light path more convergent, thereby reducing the error of experimental data.

[0051] In a specific embodiment of the utility model, Figure 1 As shown in the three-dimensional structure decomposition diagram, the filter fixing wheel 107 and the fixing frame are coaxially distributed as a whole. The coaxial arrangement can make the light beam channel naturally a straight channel. Different from the traditional equipment that requires a convex mirror for refraction, the coaxial arrangement can achieve the experimental purpose more simply and conveniently and obtain ideal experimental data.

[0052] In a specific embodiment of the utility model, Figure 1 The filter fixing wheel 107 and the fixing frame 104 shown are made of metal. Different from the traditional plastic products, the problem of aging and deformation under ultraviolet light affects the experimental results. The metal material is not easy to age and deform, which ensures the stability of the experimental process and the accuracy of the experimental results. At the same time, the characteristic of metal materials that they will oxidize and blacken can reduce the diffuse reflection of light during transmission and ensure the stability of the optical path; the surface is coated with an oxidation coating, and the oxidation and blackening of the oxidation coating can reduce the diffuse scattering of light during transmission, reduce the instability caused by the aging of the material itself, and ensure the stability of the optical path. Different from the traditional plastic products, the problem of aging and deformation under ultraviolet light affects the experimental results. The metal material is not easy to age and deform, which ensures the stability of the experimental process and the accuracy of the experimental results.

[0053] The purpose of metal materials not being easily deformed by aging is to ensure the stability of the experimental process and the accuracy of the experimental results. At the same time, this device uses the characteristics of metal materials that will oxidize and blacken, which can reduce the diffuse reflection of light during transmission and ensure the stability of the optical path; further, an oxidation coating is coated on the surface of the device. By using the oxidation and blackening of the oxidation coating, the diffuse scattering of light during transmission is reduced, and the instability caused by the aging of the material itself is reduced, thereby ensuring the stability of the optical path. Different from the problem of aging and deformation of traditional plastic products under ultraviolet rays that affects the experimental results, the metal material is not easily aged and deformed, which ensures the stability of the experimental process and the accuracy of the experimental results.

[0054] In a specific embodiment of the utility model, Figure 2 The optical fiber 116 shown is fixed to the side of the power device 101 through the optical fiber connector 102 and the optical fiber bracket 103. The optical fiber bracket fixes the optical fiber and is on the same axis as the overall device, which makes the optical fiber output more stable and the experimental data more stable.

[0055] From the above embodiments, it can be seen that the advantages of the utility model are: by using a rotatable filter fixing frame, while conveniently installing the filter, it is also possible to match filters of multiple bands, and different filters meet the experimental requirements of different bands; the light shielding problem of the optical path device is optimized, making the experimental data more stable; and the problem of easy aging of the device is improved.

[0056] The above-described embodiments only express the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above-described embodiments, and the above-described embodiments and the specification only describe the principles of the present invention. For those skilled in the art, various changes and improvements can be made without departing from the concept and scope of the present invention, and these changes and improvements fall within the scope of the present invention to be protected.

[0057] Although the embodiments of the present application have been shown and described, those skilled in the art will appreciate that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. An optical path device of an ELISA instrument, characterized in that: The optical path device comprises a filter fixing wheel (107) and a fixing frame (104); The filter fixing wheel (107) is rotatably connected to the fixing frame (104), and a plurality of filters (108) are fixed on the surface of the filter fixing wheel (107); The back side of the fixing frame (104) is connected to a power device (101).

2. The optical path device of the microplate reader according to claim 1, characterized in that: The fixing frame (104) is connected to a position sensor (105) and a positioner (106).

3. The optical path device of an ELISA instrument according to claim 1, characterized in that: A gasket (109) having its own thickness is connected between the optical filter (108) and the optical filter fixing wheel (107).

4. The optical path device of an ELISA instrument according to claim 1, characterized in that: The front side of the optical filter (108) is connected to the light source beam collector (112); The light source clustering device (112) is arranged on the light source frame (113), and the light source (114) is fixed in the optical path system via a light source fixing cover (115).

5. The optical path device of an ELISA instrument according to claim 3, characterized in that: The intervals between each fixing hole (301) of the filter fixing wheel (107) are the same.

6. The optical path device of the microplate reader according to claim 4, characterized in that: A light shielding plate (110) is provided between the filter (108) and the light source bundler (112).

7. The optical path device of an ELISA instrument according to claim 1, characterized in that: The filter fixing wheel (107) and the center of the fixing frame (104) are located on the same horizontal line.

8. The optical path device of an ELISA instrument according to claim 4, characterized in that: The filter fixing wheel (107) and the fixing frame (104) included in the optical path system are made of metal, and the surfaces are coated with an oxidation coating.

9. The optical path device of an ELISA instrument according to claim 1, characterized in that: The other side of the power device (101) is connected to an optical fiber bracket (103), and the optical fiber (116) is connected to the power device (101) via an optical fiber connector (102).

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