Tumor stem cell culture dish

Abstract

The utility model provides a tumor stem cell culture dish, which belongs to the technical field of stem cell culture dishes, and comprises a first support plate, a second support plate and a connecting plate, the culture dish is characterized by further comprising a culture dish body which is fixedly connected with the first supporting plate through a clamping mechanism and used for conveniently adjusting the overall specification size of the culture dish body according to field requirements; the second supporting plate is connected with the first supporting plate through a clamping mechanism on the culture dish body; the culture dish comprises a culture dish body, a clamping mechanism and a sliding assembly which is arranged on the culture dish body and is used for realizing the connection of a plurality of culture dish bodies; the connecting assembly is arranged on the culture dish body and is matched with the sliding assembly for use. The utility model solves the problem that the overall limitation is large because the overall specification of the culture dish is relatively deadly limited and the specification of the culture dish cannot be adjusted according to different requirements on site.

Description

Technical Field

[0001] The utility model belongs to the technical field of stem cell culture dishes, and in particular relates to a tumor stem cell culture dish. Background Art

[0002] In recent years, studies have found that there is a group of cells with normal stem cell characteristics in tumor tissues. They have their own specific expression of molecules. This group of cells is named tumor stem cells. Tumor stem cells are the root cause of tumor recurrence and metastasis. Therefore, the study of their functional mechanism has far-reaching significance for the clinical prevention and treatment of tumor diseases. However, the existing technology has a rigid limit on the size of culture dishes, and it is impossible to adjust the size of the culture dishes according to on-site needs.

[0003] Application number "CN201620756984.0" records "a tumor stem cell culture dish, comprising a dish body and a dish cover, characterized in that: a circular culture area is fixed in the center of the bottom of the dish body, and cylindrical cell culture holes of different sizes are arranged around the circular culture area; the diameter of the cell culture hole varies from 6mm to 10mm, and the height is uniformly 10mm; the circular culture area is located in the center of the dish body; the circular culture area is tangent to a certain point on the periphery of the dish body."

[0004] The above patent is based on a tumor stem cell culture dish. A circular culture area and culture wells of different sizes are fixed at the bottom of the culture dish. In this way, during the culture of tumor stem cells, the tumor cell line is first placed in the circular culture area for culture. After the stem cell spheres are formed, the single cell spheres are transferred to a culture unit of appropriate size under a microscope using a Pasteur tube. The stem cell spheres are first discretized into single cells and then amplified and cultured. As the number of cells increases, the stem cell spheres are moved to larger culture wells in turn until a stem cell line with a sufficient number of cells is obtained. In this way, the culture dish and the cell culture plate are combined into one. The whole process does not require the replacement of the cell culture plate. All steps of tumor stem cell culture can be completed with one cell culture dish. The operation is simple, consumables are saved, and the risk of cell contamination is reduced.

[0005] The above patent is designed according to the characteristics of the tumor stem cell culture process, so that the tumor stem cell culture can be completed in one culture dish, saving experimental consumables and cell culture medium, and simplifying the operation steps of tumor stem cell culture. However, the overall size of the above culture dish is relatively rigidly restricted, and the size of the culture dish cannot be adjusted according to different needs on site, resulting in greater overall limitations. Utility Model Content

[0006] The utility model aims to provide a tumor stem cell culture dish, aiming to solve the problem that the overall size of the culture dish in the prior art is relatively rigidly restricted and the size of the culture dish cannot be adjusted according to different needs on site, resulting in greater overall limitations.

[0007] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0008] A tumor stem cell culture dish, comprising:

[0009] a first support plate;

[0010] It is characterized by further comprising:

[0011] A culture dish body, which is fixedly connected to the first support plate via a clamping mechanism, and is used to facilitate adjustment of the overall size of the culture dish body according to on-site requirements;

[0012] The second supporting plate is connected to the culture dish body through a clamping mechanism on the culture dish body.

[0013] As a preferred solution of the utility model, the clamping mechanism further includes:

[0014] A sliding assembly, which is disposed on the culture dish body and is used to realize the connection of a plurality of the culture dish bodies;

[0015] A connecting component, which is disposed on the culture dish body and cooperates with the sliding component; and

[0016] The fixing component is arranged on the culture dish body and cooperates with the connecting component to realize the fixing relationship after a plurality of the culture dish bodies are connected.

[0017] As a preferred solution of the utility model, the sliding assembly includes a first T-shaped slide, a first T-shaped slider, a second T-shaped slider and a second T-shaped slide, the first T-shaped slide is opened on one side wall of the culture dish body, the first T-shaped slider is fixed to the other side wall of the culture dish body, and the first T-shaped slide and the first T-shaped slider are used in conjunction with each other, the second T-shaped slider is fixed to the side wall of the first support plate and is used in conjunction with the first T-shaped slide, and the second T-shaped slide is opened on the side wall of the second support plate and is used in conjunction with the first T-shaped slider.

[0018] As a preferred solution of the utility model, the connecting component includes a first through hole, a second through hole and a third through hole, the first through hole is opened at the end of the first T-shaped slider and passes through the first T-shaped slider, the second through hole is opened at the end of the second support plate and passes through the second T-shaped slide groove, and the third through hole is opened at the end of the second T-shaped slider and passes through the second T-shaped slider.

[0019] As a preferred solution of the utility model, the fixing assembly includes a screw and a threaded sleeve, the screw is slidably arranged on the inner walls of the first through hole, the second through hole and the third through hole, and the threaded sleeve is threadedly sleeved on the circumferential surface of the screw.

[0020] As a preferred solution of the utility model, a circular culture area is evenly opened on the top of the culture dish body.

[0021] As a preferred solution of the utility model, the bottom of the first support plate and the second support plate are both fixedly provided with feet, which are used for auxiliary support.

[0022] Compared with the prior art, the beneficial effects of the utility model are:

[0023] 1. By opening a first T-shaped slide groove on one side wall of the culture dish body, fixing a first T-shaped slider on the other side wall of the culture dish body, and the first T-shaped slide groove and the first T-shaped slider are used in conjunction with each other, the second T-shaped slider is fixed to the side wall of the first support plate and is used in conjunction with the first T-shaped slide groove, and the second T-shaped slide groove is opened on the side wall of the second support plate and is used in conjunction with the first T-shaped slider, it is convenient to increase the size of the culture dish body according to on-site needs, thereby reducing the overall limitations of the culture dish.

[0024] 2. By opening a first through hole at the end of the first T-shaped slider and passing through the first T-shaped slider, opening a second through hole at the end of the second support plate and passing through the second T-shaped slide groove, and opening a third through hole at the end of the second T-shaped slider and passing through the second T-shaped slider, a screw is slidably arranged on the inner wall of the first through hole, the second through hole and the third through hole, and a threaded sleeve is threadedly arranged on the circumferential surface of the screw, the fixing performance of the connection of multiple culture dish bodies is improved, thereby improving the overall working efficiency of the culture dishes. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:

[0026] Figure 1 This is a first-perspective stereoscopic image of a tumor stem cell culture dish of the utility model;

[0027] Figure 2 This is a first-perspective stereoscopic cross-sectional view of a tumor stem cell culture dish of the utility model;

[0028] Figure 3 This is a first-perspective three-dimensional exploded view of a tumor stem cell culture dish of the utility model;

[0029] Figure 4 This is a second-angle stereoscopic image of a tumor stem cell culture dish of the utility model;

[0030] Figure 5 This is a second-angle three-dimensional exploded view of a tumor stem cell culture dish of the utility model;

[0031] Figure 6 The utility model is a tumor stem cell culture dish Figure 3 Enlarged view of point A in the middle;

[0032] In the figure: 1, first support plate; 2, second support plate; 3, bottom foot; 4, culture dish body; 5, first T-shaped slide groove; 6, first T-shaped slider; 7, second T-shaped slider; 8, second T-shaped slide groove; 9, first through hole; 10, second through hole; 11, third through hole; 12, screw rod; 13, threaded sleeve. DETAILED DESCRIPTION

[0033] The following will clearly and completely describe the technical solutions in the embodiments of the utility model in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of them. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0034] Example

[0035] See also Figure 1-6 , the utility model provides the following technical solutions:

[0036] A tumor stem cell culture dish, which is composed of a first support plate 1, a culture dish body 4, a second support plate 2 and a clamping mechanism, is specifically described as follows:

[0037] See also Figure 1 , a first supporting plate 1;

[0038] See also Figure 2 The culture dish body 4 is fixedly connected to the first support plate 1 through a clamping mechanism, which is used to facilitate the adjustment of the overall size of the culture dish body 4 according to on-site requirements;

[0039] See also Figure 3 The second support plate 2 is connected to the culture dish body 4 through a clamping mechanism on the culture dish body 4;

[0040] The card connection mechanism also includes:

[0041] See also Figure 4, the sliding assembly is arranged on the culture dish body 4, and is used to realize the connection of multiple culture dish bodies 4. Specifically, the sliding assembly includes a first T-shaped slide groove 5, a first T-shaped slider 6, a second T-shaped slider 7 and a second T-shaped slide groove 8. The first T-shaped slide groove 5 is opened on one side wall of the culture dish body 4, the first T-shaped slider 6 is fixed to the other side wall of the culture dish body 4, and the first T-shaped slide groove 5 and the first T-shaped slider 6 are used in conjunction with each other, the second T-shaped slider 7 is fixed to the side wall of the first support plate 1 and is used in conjunction with the first T-shaped slide groove 5, and the second T-shaped slide groove 8 is opened on the side wall of the second support plate 2 and is used in conjunction with the first T-shaped slider 6;

[0042] See also Figure 5 , a connecting component is arranged on the culture dish body 4 and cooperates with the sliding component for use. Specifically, the connecting component includes a first through hole 9, a second through hole 10 and a third through hole 11. The first through hole 9 is opened at the end of the first T-shaped slider 6 and passes through the first T-shaped slider 6. The second through hole 10 is opened at the end of the second support plate 2 and passes through the second T-shaped slide groove 8. The third through hole 11 is opened at the end of the second T-shaped slider 7 and passes through the second T-shaped slider 7; and

[0043] See also Figure 3 The fixing component is arranged on the culture dish body 4 and cooperates with the connecting component to realize the fixed relationship after multiple culture dish bodies 4 are connected. Specifically, the fixing component includes a screw 12 and a threaded sleeve 13. The screw 12 is slidably arranged on the inner walls of the first through hole 9, the second through hole 10 and the third through hole 11, and the threaded sleeve 13 is threadedly sleeved on the circumferential surface of the screw 12.

[0044] See also Figure 5 A circular culture area is evenly arranged on the top of the culture dish body 4 .

[0045] See also Figure 3 The first support plate 1 and the second support plate 2 are both fixedly provided with feet 3 at the bottom, which are used for auxiliary support.

[0046] In the present embodiment: by providing the first T-shaped slide groove 5 on one side wall of the culture dish body 4, the first T-shaped slider 6 is fixed to the other side wall of the culture dish body 4, and the first T-shaped slide groove 5 and the first T-shaped slider 6 are used in conjunction with each other, the second T-shaped slider 7 is fixed to the side wall of the first support plate 1 and is used in conjunction with the first T-shaped slide groove 5, and the second T-shaped slide groove 8 is provided on the side wall of the second support plate 2 and is used in conjunction with the first T-shaped slider 6, it is convenient to increase the size of the culture dish body 4 according to on-site needs, thereby reducing the overall limitations of the culture dish.

[0047] By opening a first through hole 9 at the end of the first T-shaped slider 6 and passing through the first T-shaped slider 6, a second through hole 10 is opened at the end of the second support plate 2 and passes through the second T-shaped slide groove 8, a third through hole 11 is opened at the end of the second T-shaped slider 7 and passes through the second T-shaped slider 7, a screw 12 is slidably arranged on the inner walls of the first through hole 9, the second through hole 10 and the third through hole 11, and a threaded sleeve 13 is threadedly sleeved on the circumferential surface of the screw 12, the fixing performance of the connection of multiple culture dish bodies 4 is improved, thereby improving the overall working efficiency of the culture dishes.

[0048] The working principle and use process of the utility model are as follows: when it is desired to increase the size of the culture dish body 4, the two culture dish bodies 4 can be slidably connected through the sliding cooperation of the first T-shaped slide groove 5 and the first T-shaped slider 6. Because of the threaded cooperation of the screw 12 and the threaded sleeve 13, the screw 12 is passed through the first through hole 9 and the third through hole 11, and finally a fixed connection is achieved. The sliding cooperation relationship between the second T-shaped slider 7 and the second T-shaped slide groove 8 on the first support plate 1 and the second support plate 2 is consistent with the cooperation relationship between the first T-shaped slide groove 5 and the first T-shaped slider 6. Then, the number of culture dish bodies 4 can be increased according to the above-mentioned connection and fixing relationship, thereby improving the overall specifications of the culture dish body 4.

[0049] Finally, it should be noted that the above description is only a preferred embodiment of the utility model and is not intended to limit the utility model. Although the utility model is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions recorded in the above embodiments or replace some of the technical features therein with equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A tumor stem cell culture dish, comprising: A first support plate (1); It is characterized by further comprising: A culture dish body (4) which is fixedly connected to the first support plate (1) via a clamping mechanism, and is used to facilitate adjustment of the overall size of the culture dish body (4) according to on-site requirements; A second support plate (2) connected to the culture dish body (4) via a clamping mechanism on the culture dish body (4); The clamping mechanism comprises: A sliding assembly, which is arranged on the culture dish body (4) and is used to realize the connection of a plurality of the culture dish bodies (4); A connecting component, which is arranged on the culture dish body (4) and cooperates with the sliding component; and a fixing component, which is arranged on the culture dish body (4) and is used in cooperation with the connecting component, and is used to achieve a fixing relationship after a plurality of the culture dish bodies (4) are connected; The sliding assembly comprises a first T-shaped slide groove (5), a first T-shaped slider (6), a second T-shaped slider (7) and a second T-shaped slide groove (8), wherein the first T-shaped slide groove (5) is provided on one side wall of the culture dish body (4), the first T-shaped slider (6) is fixed on the other side wall of the culture dish body (4), and the first T-shaped slide groove (5) and the first T-shaped slider (6) are used in cooperation with each other, the second T-shaped slider (7) is fixed on the side wall of the first support plate (1) and is used in cooperation with the first T-shaped slide groove (5), and the second T-shaped slide groove (8) is provided on the side wall of the second support plate (2) and is used in cooperation with the first T-shaped slider (6); The connecting component comprises a first through hole (9), a second through hole (10) and a third through hole (11), wherein the first through hole (9) is opened at the end of the first T-shaped slider (6) and passes through the first T-shaped slider (6), the second through hole (10) is opened at the end of the second supporting plate (2) and passes through the second T-shaped slide groove (8), and the third through hole (11) is opened at the end of the second T-shaped slider (7) and passes through the second T-shaped slider (7); The fixing assembly comprises a screw rod (12) and a threaded sleeve (13); the screw rod (12) is slidably arranged on the inner walls of the first through hole (9), the second through hole (10) and the third through hole (11); and the threaded sleeve (13) is threadedly sleeved on the circumferential surface of the screw rod (12).

2. A tumor stem cell culture dish according to claim 1, characterized in that: A circular culture area is evenly arranged on the top of the culture dish body (4).

3. A tumor stem cell culture dish according to claim 2, characterized in that: The first support plate (1) and the second support plate (2) are both fixedly provided with base feet (3) at the bottom, which are used for auxiliary support.

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