Cell scratch manufacturing device
By designing a cell scratch manufacturing device containing arc-shaped slide chute and articulated design, the problem of inconsistent scratch width in the prior art is solved, the repeatability and consistency of cell scratch experiments are achieved, and the degree of standardization of the experiments is improved.
Patent Information
- Application Number
- CN202421686503.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-16
- Publication Date
- 2025-05-13
- Estimated Expiration
- 2034-07-16
AI Technical Summary
Existing cell scratch experimental equipment cannot guarantee the consistency of scratch width, making it difficult to guarantee the repeatability and consistency of the experiment.
A cell scratch manufacturing device is designed, including a base, a scratch pen and a first cross bar. Through the sliding coordination of the arc-shaped slide groove and the second cross bar, and the articulation design of the scratch pen and the first cross bar, ensuring that the gap between the scratch pen tip and the edge of the cell culture dish is always smaller than the pen body radius of the scratch pen, thereby achieving precise control of the scratch width.
Through this device, the repeatability and consistency of cell scratch experiments can be ensured, the degree of standardization of the experiments can be improved, data comparison and analysis can be facilitated, and additional damage to cells can be reduced.
Smart Images
Figure CN222861482U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of cell experimental devices, in particular to a cell scratch making device. Background Art
[0002] The cell scratch assay is a laboratory technique that studies cell migration, repair ability, and cell-to-cell interaction by scratching a cell monolayer and capturing images periodically through a time-lapse microscope. The basic principle is: an artificial blank area is created on a fused monolayer of cells, called a "scratch / wound". The cells at the edge of the scratch will gradually enter the blank area to heal the "scratch / wound". Images are captured at the beginning and periodically during the cell migration process. By measuring the scratch spacing at different time points and calculating the difference, the cell migration ability can be judged. Because it is similar to the in vitro wound healing process, it is also called the wound healing assay. It can be used to observe the effects of exogenous factors such as drugs and genes on cell migration, repair, and interaction.
[0003] The steps of the cell scratch experiment are as follows: first, draw a line on the back of the culture plate, then inoculate the cells on the culture plate for cell culture, and finally use the gun tip to scratch parallel or perpendicular to the line on the back of the culture plate.
[0004] During the scratching step, the width of each scratch should be kept consistent as much as possible. However, the scratches made by the existing cell scratch experiment equipment are difficult to ensure the consistency of the scratch width, and the scratch accuracy is unstable, which cannot ensure the repeatability and consistency of the cell scratch experiment. Utility Model Content
[0005] The utility model aims to overcome the defects and shortcomings of the prior art and provide a cell scratch making device, which can ensure the repeatability and consistency of the cell scratch experiment.
[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:
[0007] A cell scratching device comprises a base, a scratching pen and a first cross bar. A fixing frame is arranged on the base, the fixing frame comprises a first bracket and a second bracket, the two second brackets are arranged at two ends above the first bracket respectively, the first cross bar is arranged on the upper end surface of the first bracket and is fixedly connected to the first bracket. The middle part of the scratching pen is hinged on the first cross bar, and the upper end is connected to the second cross bar. An arc-shaped slide groove is opened at the upper end of the second bracket, and the second cross bar is slidably matched with the arc-shaped slide groove, so that the gap between the scratching pen tip and the edge of the cell culture dish is always smaller than the radius of the scratching pen body.
[0008] As a preferred embodiment, the cell scratching device comprises a plurality of scratching pens.
[0009] As a preferred embodiment, the cell scratching device also includes a scratch pen connector mounted on the outer wall of the scratch pen, the scratch pen connector includes a hinge block, a hinge hole corresponding to the hinge block is opened on the first cross bar, and the hinge block and the hinge hole are rotatably matched.
[0010] As a preferred embodiment, the arc-shaped slide groove is opened on the second bracket along the trajectory of the arc line, and the trajectory equation of the arc line is:
[0011]
[0012] Where l is the distance between the center of the hinge hole and the top of the scratch pen; L is the length of the scratch pen; h is the distance between the center of the hinge hole and the upper surface of the cell culture dish; θ is the angle between the scratch pen and the vertical plane.
[0013] Preferably, the cell scratch making device also includes a lifting assembly for adjusting the height of the fixing frame on the base, the base includes a bracket and a stand, the two stands are respectively arranged at both ends of the upper end surface of the bracket, and the fixing frame is slidably matched with the stand through the lifting assembly.
[0014] Preferably, the lifting assembly includes a track, a slider and a fastener. The track is arranged on the outside of the vertical frame, and the slider is arranged on the inside of the first bracket at a position corresponding to the track. The slider and the track are slidably matched. A positioning hole is provided on the first bracket, and the fastener passes through the positioning hole and abuts against the track to limit the relative sliding between the first bracket and the vertical frame.
[0015] As a preference, the lifting assembly further comprises a reset structure, one end of which is connected to the top of the stand, and the other end of which is connected to the first bracket.
[0016] As a preferred embodiment, the reset structure is detachably installed between the top of the stand and the first bracket.
[0017] As a preferred embodiment, the reset structure includes a spring and a hook, and the two hooks are respectively arranged at both ends of the spring. A first connecting part corresponding to the hook is arranged on the top of the vertical frame, and a second connecting part corresponding to the hook is arranged on the first bracket. The hook at the head end of the spring is buckled on the first connecting part, and the hook at the end of the spring is buckled on the second connecting part.
[0018] As a preferred embodiment, the bracket is a U-shaped bracket.
[0019] In general, the utility model has the following advantages:
[0020] 1. Precise positioning and control. The sliding cooperation between the arc-shaped slide and the second crossbar, as well as the hinged design between the scratch pen and the first crossbar, ensure that the tip of the scratch pen can achieve precise scratches on the cell culture dish. If the gap between the tip of the scratch pen and the edge of the cell culture dish is larger than the radius of the scratch pen body, the scratch may become wider due to the lateral movement of the tip of the scratch pen, resulting in different scratch widths. The cell scratch making device of the utility model ensures that the width of the scratch is only determined by the tip of the pen by keeping the gap between the tip of the pen and the edge of the cell culture dish smaller than the radius of the pen body, thereby maintaining the consistency of the scratch.
[0021] 2. Standardization of scientific research. The cell scratch manufacturing device of the utility model helps to achieve the standardization of cell scratch experiments, ensure the comparability and reliability of experimental results, and is of great significance to biological research such as cell migration and wound healing.
[0022] 3. Multiple scratch pen configuration. The cell scratch making device of the utility model comprises multiple scratch pens (at least three). Through the hinged connection between the scratch pen connector and the first cross bar, multiple parallel scratches can be made on the cell culture dish at the same time, thereby improving the experimental efficiency and consistency.
[0023] 4. Height adjustment and locking. The lifting assembly allows the user to easily adjust the height of the fixed frame to adapt to cell culture dishes of different sizes. At the same time, the contact depth between the scratch pen and the culture dish can be simply adjusted to facilitate adjustment to different experimental needs; and the height can be locked through fasteners to ensure the stability of the scratching process.
[0024] 5. Reset structure: The reset structure (such as spring and hook) ensures that the device can automatically reset to the initial state after each operation is completed, reducing the time of manual adjustment and increasing the convenience of operation.
[0025] 6. Durability and maintenance. The structural design of the cell scratch manufacturing device of the utility model takes durability and easy maintenance into consideration, such as the use of threaded fasteners and a spring connected by a hook in the reset structure, which facilitates regular inspection and replacement of worn parts.
[0026] 7. Adaptability and versatility: The bracket is designed as a U-shaped bracket, and its shape and size match the cell culture dish, which not only improves the versatility of the device, but also better fixes the culture dish and reduces the risk of movement during operation. BRIEF DESCRIPTION OF THE DRAWINGS
[0027] Figure 1 It is a structural schematic diagram of the cell scratch making device of the utility model;
[0028] Figure 2 It is a structural schematic diagram of the base of the utility model;
[0029] Figure 3 It is a structural schematic diagram of a fixing frame of the utility model;
[0030] Figure 4 It is a structural schematic diagram of the connection between the second crossbar, the scratch pen connecting piece and the scratch pen of the utility model;
[0031] Figure 5 It is a structural schematic diagram of the arc chute of the utility model;
[0032] Figure 6 It is a structural schematic diagram of the lifting assembly of the utility model;
[0033] Wherein: 1: base, 11: bracket, 12: stand, 2: scratch pen, 3: first cross bar, 31: hinge hole, 4: fixing frame, 41: first bracket, 411: positioning hole, 42: second bracket, 43: arc-shaped slide groove, 5: second cross bar, 6: scratch pen connector, 61: hinge block, 7: lifting assembly, 71: track, 72: slider, 73: fastener, 74: reset structure, 741: spring, 742: hook, 8: cell culture dish. DETAILED DESCRIPTION
[0034] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific implementation methods.
[0035] like Figure 1 As shown, a cell scratch manufacturing device includes a base, a scratch pen and a first crossbar; a fixing frame is arranged on the base, the fixing frame includes a first bracket and a second bracket, the two second brackets are respectively arranged at two ends above the first bracket, and the first crossbar is arranged on the upper end surface of the first bracket and is fixedly connected to the first bracket. The cell scratch manufacturing device of the utility model includes three first crossbars, the three first crossbars are evenly distributed on the upper end surface of the first bracket, and the second bracket is fixedly connected to the upper end surface of the first crossbar located at the end of the first bracket.
[0036] like Figure 3 and Figure 4 As shown, the middle part of the scratch pen is hinged on the first cross bar, and the upper end is connected to the second cross bar. The cell scratch manufacturing device also includes a scratch pen connector sleeved on the outer wall of the scratch pen, the scratch pen connector includes a hinge block, and the first cross bar is provided with a hinge hole corresponding to the hinge block, and the hinge block and the hinge hole are rotatably matched. The scratch pen connector is provided with a mounting hole, the scratch pen is inserted into the mounting hole, and there is a clearance fit between the scratch pen and the mounting hole, and the scratch pen can slide in the mounting hole along the axial direction of the mounting hole; the hinge hole is provided in the middle part of the first cross bar, and the hinge block is inserted into the hinge hole, and there is a clearance fit or transition fit between the hinge block and the hinge hole.
[0037] The upper end of the second bracket is provided with an arc-shaped slide groove, and the second cross bar is slidably matched with the arc-shaped slide groove, so that the gap between the tip of the scratch pen and the edge of the cell culture dish is always smaller than the radius of the body of the scratch pen. The upper end of the scratch pen is provided with a connecting hole, and the end of the second cross bar is inserted into the arc-shaped slide groove through the connecting hole. There is a clearance fit between the second cross bar and the arc-shaped slide groove, and the second cross bar drives the scratch pen to slide circumferentially along the arc-shaped slide groove with the hinge hole as the center of the circle.
[0038] like Figure 5 As shown, the arc-shaped slide groove is opened on the second bracket according to the trajectory of the arc line, and the trajectory equation of the arc line is:
[0039]
[0040] Where l is the distance between the center of the hinge hole and the top of the scratch pen; L is the length of the scratch pen; h is the distance between the center of the hinge hole and the upper surface of the cell culture dish; θ is the angle between the scratch pen and the vertical plane. l and θ are variables. By controlling the proportional relationship between the swing amount and the extension amount of the scratch pen, the motion trajectory of the scratch pen tip is made a straight line.
[0041] In this embodiment, an arc-shaped slide groove is arranged at the upper end of the second bracket, which determines the movement path of the scratch pen. The second cross bar is connected to the upper end of the scratch pen. The sliding of the second cross bar in the arc-shaped slide groove can guide the scratch pen to make smooth and continuous scratches on the cell culture dish. The scratch pen connector is sleeved on the outer wall of the scratch pen. The scratch pen connector is inserted into the hinge hole on the first cross bar through a hinge block to form a hinge point. The hinge point allows the scratch pen connector to rotate on the first cross bar, thereby adjusting the angle between the scratch pen and the cell culture dish. The mounting hole is opened on the scratch pen connector, and the scratch pen is inserted into the mounting hole. There is a clearance fit between the scratch pen and the mounting hole, allowing the scratch pen to slide along the inner edge of the mounting hole while maintaining a hinged relationship with the first cross bar.
[0042] When the second cross bar moves along the arc-shaped slide groove, the scratch pen rotates around the hinge hole on the first cross bar, thereby changing the angle and position of the scratch pen on the cell culture dish, and slides in the mounting hole of the scratch pen connector, thereby changing the depth of the scratch on the cell culture dish.
[0043] It ensures that the distance between the tip of the scratch pen and the edge of the cell culture dish is always smaller than the radius of the pen body of the scratch pen, and avoids the part other than the tip of the pen touching the cell culture dish during the scratching process. It not only ensures that the width of the scratches is consistent, thereby maintaining the consistency of the scratches, reducing the differences between operators, improving the standardization of the experiment, and facilitating data comparison and analysis, but also avoids bringing microorganisms or other substances on the edge of the cell culture dish into the scratch area, preventing cell contamination and affecting the purity of the experimental results. It can also reduce additional damage to cells, prevent scratching cells or destroying the integrity of the cell layer, and avoid affecting the normal migration and proliferation of cells.
[0044] The cell scratching device comprises a plurality of scratching pens. The cell scratching device of the utility model comprises three scratching pens and three scratching pen connecting pieces, each scratching pen is sleeved with a scratching pen connecting piece, and each scratching pen connecting piece is correspondingly hinged on a first cross bar.
[0045] The cell scratch making device also includes a lifting assembly for adjusting the height of the fixing frame on the base, the base includes a bracket and a stand, the two stand are respectively arranged at the two ends of the upper end surface of the bracket, and the fixing frame is slidably matched with the stand through the lifting assembly. The cell scratch making device of the utility model includes two lifting assemblies, and the two lifting assemblies are symmetrically arranged at the two ends of the cell scratch making device.
[0046] like Figure 2 , Figure 3 and Figure 6 As shown, the lifting assembly includes a track, a slider and a fastener. The track is arranged on the outside of the stand, and the slider is arranged on the inside of the first bracket at a position corresponding to the track. The slider and the track are slidably matched. The track is fixedly mounted on the stand, and the slider is fixedly mounted on the first bracket. The track and the slider are arranged opposite to each other, and the slider drives the first bracket to slide up and down on the stand along the track.
[0047] The first bracket is provided with a positioning hole, and the fastener passes through the positioning hole and abuts against the track to limit the relative sliding between the first bracket and the stand. The fastener is a bolt, and a thread is provided in the positioning hole, and the fastener is threadedly connected with the positioning hole.
[0048] like Figure 6As shown, the lifting assembly also includes a reset structure, one end of which is connected to the top of the stand, and the other end is connected to the first bracket. The reset structure is detachably installed between the top of the stand and the first bracket. The reset structure includes a spring and a hook, and the two hooks are respectively arranged at the two ends of the spring. The top of the stand is provided with a first connecting part corresponding to the hook, and the first bracket is provided with a second connecting part corresponding to the hook. The hook at the head end of the spring is hooked on the first connecting part, and the hook at the end of the spring is hooked on the second connecting part. The first connecting part includes a short shaft fixed to the top of the stand; the second connecting part includes a hook hole opened on the upper end surface of the first bracket.
[0049] The bracket is a U-shaped bracket, and the shape and size of the bracket are adapted to the shape and size of the cell culture dish.
[0050] The use steps of this utility model:
[0051] Single operation object:
[0052] S1. Prepare the device, make sure the opening of the base faces the user, and install only one scratch pen.
[0053] S2. Height adjustment: rotate and loosen the fasteners. The elasticity of the reset structure will make the fixing bracket rise to the highest position of the base in the vertical direction.
[0054] S3. Place the cell culture dish manually in the base, ensure that the left outer wall of the cell culture dish is in close contact with the left inner wall of the bracket, and the rear outer wall is in close contact with the rear inner wall of the bracket, so that the center of the cell culture dish is located on the axis of the scratch pen.
[0055] S4. With the pen tip touching, hold the first bracket with one hand and press down gently to move the fixed bracket slowly downward.
[0056] S5. Line drawing guide: when the tip of the scratch pen falls into the range of the cell culture dish, the other hand pushes and pulls the second crossbar, and when the fixing frame continues to move downward, the tip of the scratch pen is brought close to the inner side wall of the cell culture dish, and then the fastener is rotated and tightened to fix the fixing frame.
[0057] S6, marking action, pushing the second crossbar to move the marking pen back and forth, the tip of the marking pen moves from one side wall of the cell culture dish to the other side wall, completing the marking action.
[0058] S7, reset, rotate and loosen the fastener, the fixing frame automatically moves upward under the tension of the reset structure, and the scratch pen leaves the range of the cell culture dish.
[0059] S8. Take out the cell culture dish and pull it out horizontally from the base to complete a streaking operation.
[0060] Multiple operation objects:
[0061] S1. Prepare the device, ensure that the opening of the base faces the user, and install three scratch pens.
[0062] S2. Height adjustment: rotate and loosen the fasteners. The elasticity of the reset structure will make the fixing bracket rise to the highest position of the base in the vertical direction.
[0063] S3. Place the cell culture dish manually in the base, ensure that the left outer wall of the cell culture dish is in close contact with the left inner wall of the bracket, and the rear outer wall is in close contact with the rear inner wall of the bracket, so that the center of the cell culture dish is located on the axis of the scratch pen.
[0064] S4. With the pen tip touching, hold the first bracket with one hand and press down gently to move the fixed bracket slowly downward.
[0065] S5. Scribing guide: when the tips of all the scratch pens fall into the range of the cell culture dish, use the other hand to push and pull the second crossbar. When the fixed frame continues to move downward, make the tips of all the scratch pens close to the inner side wall of the cell culture dish, and then rotate and tighten the fasteners to fix the fixed frame.
[0066] S6, marking action, pushing the second cross bar to move all the scratch pens back and forth, and the pen tips of all the scratch pens move from one side wall of the cell culture dish to the other side wall to complete the marking action.
[0067] S7, reset, rotate and loosen the fastener, the fixing frame automatically moves upward under the tension of the reset structure, and all the scratch pens leave the range of the cell culture dish.
[0068] S8. Take out the cell culture dish and pull it out horizontally from the base to complete a streaking operation.
[0069] The above-mentioned embodiments only express several implementation methods of the utility model, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the patent of the utility model. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the utility model, and these all belong to the protection scope of the utility model. Therefore, the protection scope of the utility model patent shall be based on the attached claims.
Claims
1. A cell scratching device, characterized in that: It comprises a base, a scratch pen and a first cross bar; a fixing frame is arranged on the base, the fixing frame comprises a first bracket and a second bracket, the two second brackets are arranged at two ends above the first bracket respectively, the first cross bar is arranged on the upper end surface of the first bracket and is fixedly connected to the first bracket; the middle part of the scratch pen is hinged on the first cross bar, and the upper end is connected with the second cross bar; an arc-shaped slide groove is arranged at the upper end of the second bracket, and the second cross bar is slidably matched with the arc-shaped slide groove, so that the gap between the tip of the scratch pen and the edge of the cell culture dish is always smaller than the radius of the pen body of the scratch pen.
2. A cell scratching device according to claim 1, characterized in that: The cell scratch making device comprises a plurality of scratch pens.
3. A cell scratching device according to claim 1, characterized in that: The cell scratching device also includes a scratch pen connector sleeved on the outer wall of the scratch pen, the scratch pen connector includes a hinge block, a hinge hole corresponding to the hinge block is opened on the first crossbar, and the hinge block and the hinge hole are rotatably matched.
4. A cell scratching device according to claim 3, characterized in that: The arc chute is opened on the second bracket along the arc trajectory, and the arc trajectory equation is: Where l is the distance between the center of the hinge hole and the top of the scratch pen; L is the length of the scratch pen; h is the distance between the center of the hinge hole and the upper surface of the cell culture dish; θ is the angle between the scratch pen and the vertical plane.
5. A cell scratching device according to claim 1, characterized in that: The cell scratch making device also includes a lifting assembly for adjusting the height of the fixing frame on the base. The base includes a bracket and a stand. The two stands are respectively arranged at both ends of the upper end surface of the bracket. The fixing frame slides with the stand through the lifting assembly.
6. A cell scratching device according to claim 5, characterized in that: The lifting assembly includes a track, a slider and a fastener. The track is arranged on the outside of the vertical frame, and the slider is arranged on the inside of the first bracket at a position corresponding to the track. The slider and the track are slidably matched. A positioning hole is arranged on the first bracket, and the fastener passes through the positioning hole and abuts against the track to limit the relative sliding between the first bracket and the vertical frame.
7. A cell scratching device according to claim 6, characterized in that: The lifting assembly also includes a reset structure, one end of which is connected to the top of the stand, and the other end of which is connected to the first bracket.
8. A cell scratching device according to claim 7, characterized in that: The reset structure is detachably installed between the top of the stand and the first bracket.
9. A cell scratching device according to claim 8, characterized in that: The reset structure includes a spring and a hook, and the two hooks are respectively arranged at the two ends of the spring. A first connecting part corresponding to the hook is arranged on the top of the vertical frame, and a second connecting part corresponding to the hook is arranged on the first bracket. The hook located at the head end of the spring is buckled on the first connecting part, and the hook located at the end of the spring is buckled on the second connecting part.
10. The cell scratch making device according to claim 1, characterized in that: The bracket is a U-shaped bracket.