Antibody incubation frame for immunohistochemistry and immunofluorescence staining

Abstract

The utility model relates to an antibody incubation frame for immunohistochemistry and immunofluorescence staining, which comprises an outer frame, transverse plates and auxiliary plates, a plurality of transverse plates are arranged in the outer frame, the transverse plates are connected with the outer frame in a sliding manner, the auxiliary plates are connected with the outer frame in an inserting manner, and glass slides are placed between the adjacent transverse plates and the outer frame. Compared with the prior art, the antibody incubation frame disclosed by the utility model has the advantages that the incubation frame can be adjusted according to the position of a tissue sample through the incubation frame structure provided with the outer frame, the movable transverse plate and the auxiliary plate; the frame is easy to disassemble and has adjustability; therefore, the effect of a histochemical pen in the traditional immunohistochemical experiment operation process can be realized; and the combination mode is simple and can be repeatedly used, so that the repeatability of each experiment is improved, and the personalized operation and expansibility requirements of similar related experiments can be increased.

Description

Technical Field

[0001] The utility model relates to the field of immunohistochemistry and immunofluorescence experiments, in particular to an antibody incubation frame for immunohistochemistry and immunofluorescence staining. Background Art

[0002] Immunohistochemistry and immunofluorescence staining are performed by testing the obtained pathological tissues, using the principle of antigen-antibody binding reaction in immunology, and detecting whether there are some specific antigens in the corresponding tissue cells through labeled antibodies, and can also quantify and locate these antigens. Immunohistochemistry has a wide range of applications in medical research and pathological diagnosis. This test can be used to detect inflammation and infection in tissues, and can also be used to identify different types of tumors, as well as identify and quantify specific cell types in tissues.

[0003] In immunohistochemistry experiments, since antibodies are relatively expensive, it is usually necessary to use an immunohistochemistry pen to reduce the antibody incubation area during antibody incubation. Next, the tissue slide incubated with antibodies is placed in a wet box and gently placed in a constant temperature incubator for incubation. The slight shaking of the experimenter during the storage process can easily cause the antibody liquid to flow out of the area circled by the histochemical pen and merge with other droplets. In the final sealing process, immunofluorescence has no transparent process of the transparent agent, resulting in residual marks of the histochemical pen on the stained slide. If the traces of the histochemical pen are not processed, during the shooting of the immunofluorescence results, the cover glass and the slide cannot be completely located in the same plane due to the traces of the histochemical pen, and the high-power microscope shooting will result in unclear field of view, which seriously affects the quality of the experimental results. Utility Model Content

[0004] The utility model aims to provide an antibody incubation frame for immunohistochemistry and immunofluorescence staining in order to solve the problems in the prior art that liquid is easy to diffuse during antibody incubation and the fit in immunofluorescence sealing is not enough in immunohistochemistry and immunofluorescence experiments.

[0005] The purpose of the utility model can be achieved through the following technical solutions:

[0006] An antibody incubation frame for immunohistochemistry and immunofluorescence staining comprises an outer frame, a transverse plate and an auxiliary plate. The outer frame is provided with a plurality of transverse plates, which are slidably connected to the outer frame. The auxiliary plate is plug-connected to the outer frame, and slides are placed between adjacent transverse plates and the outer frame.

[0007] In one embodiment of the utility model, the outer frame includes a first baffle, a second baffle, a third baffle and a fourth baffle, and the first baffle, the second baffle, the third baffle, the fourth baffle and the first baffle are sequentially connected to form a rectangular frame structure, and the adjacent horizontal plates, the second baffles and the fourth baffles are used to place glass slides.

[0008] In an implementation manner of the present invention, the distance between the inner sides of the second baffle and the fourth baffle of the outer frame is equal to the length of the long side of the slide.

[0009] In one embodiment of the utility model, a wire groove is provided at the upper end of the outer frame, two wire grooves are symmetrically arranged on the inner sides of the second baffle plate and the fourth baffle plate of the outer frame, and the transverse plate is slidably connected to the wire groove.

[0010] In an embodiment of the utility model, two sliding blocks are symmetrically arranged on the transverse plate, and the sliding blocks are slidably connected to the wire trough.

[0011] In one embodiment of the utility model, a buckle is provided at the bottom end of the outer frame, and the buckle is arranged around the inner side of the bottom of the first baffle, the second baffle, the third baffle and the fourth baffle, and the buckle is used to clamp the slide.

[0012] In an embodiment of the present utility model, grooves are symmetrically provided at both ends of the auxiliary plate, and the grooves are plug-connected with the first baffle plate and the third baffle plate of the outer frame.

[0013] In one embodiment of the utility model, a fence structure is provided at the lower end of the auxiliary plate, the fence structure is provided between two grooves, and the fence structure is plug-connected to the transverse plate.

[0014] As a preferred technical solution, a plurality of auxiliary plates are provided.

[0015] As a preferred technical solution, the size of the auxiliary plate can be selected according to actual needs to avoid interference with tissues in different positions; in specific implementation, the auxiliary plate can be inserted at any position of the horizontal plate due to its dense fence structure and assist in maintaining the antibody incubation area.

[0016] In one embodiment of the present invention, the materials of the outer frame, the transverse plate and the auxiliary plate are all hydrophobic soft materials.

[0017] In one embodiment of the present invention, the material of the outer frame, the cross plate and the auxiliary plate is selected from any one of PDMS, PE, PAA and rubber.

[0018] Compared with the prior art, the utility model has the following beneficial effects:

[0019] 1. The antibody incubation frame provided by the utility model can adjust the incubation frame according to the position of the tissue sample through the incubation frame structure provided with an outer frame, a movable horizontal plate and an auxiliary plate; and the frame can be easily disassembled and has adjustability; thereby, the function of the histochemical pen in the traditional immunohistochemical experiment operation process can be realized.

[0020] 2. The combination of the outer frame, movable horizontal plate, auxiliary plate and slide of the antibody incubation frame provided by the utility model is simple and reusable, which improves the repeatability of each experiment and can also increase the personalized operation and scalability requirements of similar related experiments.

[0021] 3. The auxiliary plate of the antibody incubation frame provided by the utility model has variability. After the position of the horizontal plate is determined, the auxiliary plate can be buckled on the outer frame and the horizontal plate in multiple directions according to the two-dimensional positioning of the tissue, further reducing the area of ​​tissue incubation antibodies.

[0022] 4. The antibody incubation frame provided by the utility model can avoid the easy loss of biological samples during transfer, reduce the input of antibody solution, avoid unnecessary waste, and reduce experimental costs.

[0023] 5. The antibody incubation frame provided by the utility model can avoid the negative impact of the histochemistry pen, greatly reduce the manual operation precision of antibody incubation, and facilitate experimenters to quickly get started; at the same time, it significantly reduces the operating accuracy of experimenters, improves experimental efficiency, and effectively solves the defects brought by the existing technology. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a schematic diagram of the overall structure of the antibody incubation frame of the utility model;

[0025] Figure 2 A top view of the outer frame provided by the utility model;

[0026] Figure 3 This is a top view of the auxiliary plate provided by the utility model.

[0027] Explanation of the accompanying figure numbers: 1. outer frame, 2. wire trough, 3. buckle, 4. horizontal plate, 5. auxiliary plate, 6. groove. DETAILED DESCRIPTION

[0028] The present invention is described in detail below in conjunction with the accompanying drawings and specific embodiments. This embodiment is implemented based on the technical solution of the present invention, and provides a detailed implementation method and a specific operation process, but the protection scope of the present invention is not limited to the following embodiments.

[0029] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0030] In the description of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0031] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0032] In conjunction with the accompanying drawings, some embodiments of the present invention are described in detail below. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.

[0033] Example 1

[0034] See also Figures 1 to 3 The present embodiment provides an antibody incubation frame for immunohistochemistry and immunofluorescence staining, comprising an outer frame 1, a horizontal plate 4 and an auxiliary plate 5. The outer frame 1 is provided with a plurality of horizontal plates 4, and the plurality of horizontal plates 4 are slidably connected to the outer frame 1. The auxiliary plate 5 is plug-connected to the outer frame 1, and the space between adjacent horizontal plates 4 and the outer frame 1 is used for placing glass slides.

[0035] In this embodiment, the outer frame 1 includes a first baffle, a second baffle, a third baffle and a fourth baffle. The first baffle, the second baffle, the third baffle, the fourth baffle and the first baffle are sequentially connected to form a rectangular frame structure, and the adjacent horizontal plates 4, the second baffle and the fourth baffle are used to place glass slides.

[0036] In this embodiment, the distance between the inner sides of the second baffle and the fourth baffle of the outer frame 1 is equal to the length of the long side of the slide.

[0037] In this embodiment, a wire groove 2 is provided at the upper end of the outer frame 1 , and two wire grooves 2 are symmetrically arranged on the inner sides of the second baffle plate and the fourth baffle plate of the outer frame 1 , and the transverse plate 4 is slidably connected to the wire groove 2 .

[0038] In this embodiment, two sliding blocks are symmetrically provided on the transverse plate 4 , and the sliding blocks are slidably connected to the wire trough 2 .

[0039] In this embodiment, a buckle 3 is provided at the bottom end of the outer frame 1. The buckle 3 is arranged around the inner sides of the bottoms of the first baffle, the second baffle, the third baffle and the fourth baffle, and the buckle 3 is used to clamp the slide.

[0040] In this embodiment, grooves 6 are symmetrically provided at both ends of the auxiliary plate 5 , and the grooves 6 are plug-connected to the first baffle plate and the third baffle plate of the outer frame 1 .

[0041] In this embodiment, a fence structure is provided at the lower end of the auxiliary plate 5 , and the fence structure is provided between two grooves 6 . The fence structure is plug-connected to the transverse plate 4 .

[0042] In this embodiment, the outer frame 1, the transverse plate 4 and the auxiliary plate 5 are all made of rubber material.

[0043] In addition, this embodiment also provides a method for using an antibody incubation frame for immunohistochemistry and immunofluorescence staining, and the specific steps are as follows:

[0044] S1. In immunohistochemistry and immunofluorescence experiments, the position of the horizontal plate 4 is adjusted according to the size and position of the tissue to be tested; after the horizontal plate 4 is moved, the buckle 3 is engaged with the glass slide to reduce the lateral area of ​​antibody incubation; after the outer frame 1 fits with the glass slide, the horizontal plate 4 can form a sealing structure with the plane of the glass slide.

[0045] S2. After the position of the horizontal plate 4 is determined, the auxiliary plate 5 is buckled on the outer frame 1 according to the two-dimensional positioning of the tissue to be tested, so as to further reduce the area of ​​the tissue to be tested for incubation with antibodies and avoid droplet diffusion.

[0046] S3. After the antibody incubation area is determined, add the antibody solution and transfer to a wet box; due to the hydrophobicity and height of the outer frame 1, there is no need to worry about the phenomenon of liquid fusion when staining multiple antibodies.

[0047] S4. After the antibody incubation is completed, the washing solution can be directly sucked out and added to the antibody incubated tissue. If a larger amount of washing liquid is needed, the auxiliary plate 5 can be removed to increase the washing liquid volume of the tissue.

[0048] S5. After the immunohistochemistry or fluorescent staining is completed, the antibody incubation frame is removed and the coverslip can be directly sealed without the unevenness observed under a high-power microscope.

[0049] Example 2

[0050] This embodiment provides an antibody incubation frame for immunohistochemistry and immunofluorescence staining, which is the same as the first embodiment except that two auxiliary plates 5 are provided and the space between the two auxiliary plates 5 and the adjacent horizontal plates 4 is used to place tissue samples.

[0051] The above description of the embodiments is to facilitate the understanding and use of the utility model by those skilled in the art. It is obvious that those familiar with the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the utility model is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the utility model without departing from the scope of the utility model should be within the scope of protection of the utility model.

Claims

1. An antibody incubation frame for immunohistochemistry and immunofluorescence staining, characterized in that: The invention comprises an outer frame (1), a transverse plate (4) and an auxiliary plate (5); a plurality of transverse plates (4) are arranged inside the outer frame (1); the plurality of transverse plates (4) are slidably connected to the outer frame (1); the auxiliary plate (5) is plug-connected to the outer frame (1); and a glass slide is placed between adjacent transverse plates (4) and the outer frame (1).

2. The antibody incubation frame for immunohistochemistry and immunofluorescence staining according to claim 1, characterized in that: The outer frame (1) comprises a first baffle, a second baffle, a third baffle and a fourth baffle, wherein the first baffle, the second baffle, the third baffle, the fourth baffle and the first baffle are sequentially connected to form a rectangular frame structure, and the space between the adjacent transverse plates (4), the second baffle and the fourth baffle is used to place a glass slide.

3. The antibody incubation frame for immunohistochemistry and immunofluorescence staining according to claim 2, characterized in that: The distance between the inner sides of the second baffle and the fourth baffle of the outer frame (1) is equal to the length of the long side of the slide.

4. The antibody incubation frame for immunohistochemistry and immunofluorescence staining according to claim 3, characterized in that: A wire groove (2) is provided at the upper end of the outer frame (1), two wire grooves (2) are symmetrically arranged on the inner sides of the second baffle plate and the fourth baffle plate of the outer frame (1), and the transverse plate (4) is slidably connected to the wire groove (2).

5. The antibody incubation frame for immunohistochemistry and immunofluorescence staining according to claim 4, characterized in that: Two sliding blocks are symmetrically arranged on the transverse plate (4), and the sliding blocks are slidably connected to the wire trough (2).

6. The antibody incubation frame for immunohistochemistry and immunofluorescence staining according to claim 2, characterized in that: A buckle (3) is provided at the bottom end of the outer frame (1), and the buckle (3) is arranged around the inner sides of the bottoms of the first baffle, the second baffle, the third baffle and the fourth baffle, and the buckle (3) is used to clamp the slide.

7. The antibody incubation frame for immunohistochemistry and immunofluorescence staining according to claim 2, characterized in that: Grooves (6) are symmetrically provided at both ends of the auxiliary plate (5), and the grooves (6) are plug-connected to the first baffle plate and the third baffle plate of the outer frame (1).

8. The antibody incubation frame for immunohistochemistry and immunofluorescence staining according to claim 7, characterized in that: The lower end of the auxiliary plate (5) is provided with a fence-like structure, the fence-like structure is arranged between the two grooves (6), and the fence-like structure is plug-connected to the transverse plate (4).

9. The antibody incubation frame for immunohistochemistry and immunofluorescence staining according to claim 1, characterized in that: The materials of the outer frame (1), the transverse plate (4) and the auxiliary plate (5) are all hydrophobic soft materials.

10. An antibody incubation frame for immunohistochemistry and immunofluorescence staining according to claim 9, characterized in that: The material of the outer frame (1), the horizontal plate (4) and the auxiliary plate (5) is selected from any one of PDMS, PE, PAA and rubber.

Images