Antibody incubation box for immunoblotting experiment

Abstract

The utility model relates to an antibody incubation box for an immunoblotting experiment. The antibody incubation box comprises an incubation layer, the incubation layer comprises an incubation layer main body, a slot type tray, a tray buckle, a liquid inlet, a liquid outlet and a sealing plug; the liquid inlet and the liquid outlet are respectively formed in two sides of the incubation layer main body; the slot type tray is connected with the incubation layer main body; the tray buckle is arranged on the slot type tray; and the sealing plugs are detachably connected to the liquid inlet and the liquid outlet. The position of the liquid inlet on the same incubation layer is higher than that of the liquid outlet. And the plurality of incubation layers are stacked to form the box body. Compared with the prior art, the antibody incubation box provided by the utility model is provided with the liquid inlet and the liquid outlet, so that an antibody solution can be injected and recycled in a relatively sealed environment, the mutual pollution between antibodies can be reduced, the contact between a membrane and external air is also reduced, and the influence on subsequent color development is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of biological experiments, in particular to an antibody incubation box for immunoblotting experiments. Background Art

[0002] Immunoblotting, also known as protein blotting or Western Blot, is a commonly used experimental method in molecular biology. Its basic principle is to stain cells or biological tissue samples treated by gel electrophoresis with specific antibodies, and obtain information on the expression of specific proteins in the analyzed cells or tissues by analyzing the position and depth of staining, that is, the target protein is separated by electrophoresis and transferred to a PVDF membrane, then hybridized with known protein antibodies, and finally the target protein is qualitatively and quantitatively analyzed by the color development reaction of the enzyme system. It mainly includes 5 steps: electrophoresis, transfer, blocking, hybridization and color development, among which the blocking and hybridization process is also called the antibody incubation process.

[0003] During incubation, the blocked PVDF membrane is cut and placed in an incubation box, and a primary antibody diluent is added. After incubation, the primary antibody diluent is poured out again, a membrane washing solution is added to wash the membrane, and then the membrane washing solution is poured out again, and a secondary antibody diluent is added for secondary antibody incubation. The whole process requires repeated addition and pouring of the liquid in the pouring box. The process is repetitive and complicated, and the intervals between adjacent compartments are not complete. There is a risk of mutual contamination of antibodies during incubation, and there is also a risk of contamination of the PVDF membrane by the external environment.

[0004] For example, CN220207621U discloses an antibody incubation box for immunoblotting experiments, in which the space inside the box is divided into multiple independent spaces by slots and partitions to accommodate different strips. At the same time, the partitions can be inserted into slots at different positions according to the size of the strips to change the size of each grid in the box. The whole process requires repeated addition and pouring of the liquid in the box, which is a repetitive and complicated process. In addition, the intervals between adjacent compartments are not complete, and there is a risk of mutual contamination of antibodies during incubation.

[0005] Therefore, there is an urgent need for an antibody incubation kit for immunoblotting experiments that can effectively prevent the target protein from being contaminated during incubation. Utility Model Content

[0006] The purpose of the utility model is to provide an antibody incubation box for immunoblotting experiments in order to overcome the defects of the above-mentioned prior art. The liquid inlet and the liquid outlet are arranged so that the antibody solution can be injected and recovered in a relatively sealed environment, which can reduce the mutual contamination between antibodies and the contact between the membrane and the outside air, thereby reducing the influence on the subsequent color development.

[0007] The purpose of the utility model can be achieved through the following technical solutions:

[0008] The utility model aims to provide an antibody incubation box, which comprises an incubation layer; the incubation layer comprises an incubation layer body, a slot tray, a tray buckle, a liquid inlet, a liquid outlet and a sealing plug; each incubation layer is provided with two perfusion ports as liquid inlet and outlet units, the two perfusion ports are respectively a liquid inlet and a liquid outlet, ensuring that each incubation layer has an independent liquid inlet and outlet unit, the liquid inlet and the liquid outlet are respectively arranged on both sides of the incubation layer body; the slot tray is connected to the incubation layer body to carry a PVFD film; the tray buckle is arranged on the slot tray to fix the PVFD film; the sealing plug is detachably connected to the liquid inlet and the liquid outlet, and the liquid inlet and the liquid outlet are both sealed by the sealing plug to prevent internal liquid leakage during the incubation process.

[0009] Furthermore, the size of the sealing plug matches the size of the liquid inlet and the liquid outlet.

[0010] Furthermore, the perfusion port on one side is located lower and is responsible for liquid outflow, while the perfusion port on the other side is located higher and is responsible for liquid inflow, that is, the setting position of the liquid inlet on the same incubation layer is higher than the setting position of the liquid outlet.

[0011] Furthermore, a plurality of incubation layers are stacked to form a box body, and each incubation layer has the same configuration.

[0012] Furthermore, the slot-type tray is a tray made of rubber material.

[0013] Furthermore, the sealing plug is a sealing plug made of rubber material.

[0014] Furthermore, the incubation layer body is a rectangular parallelepiped shell.

[0015] Furthermore, a slide groove is provided at the bottom of the incubation layer body; a protrusion is provided at the bottom of the slot-type tray; the protrusion cooperates with the slide groove; and the slot-type tray is slidably connected to the incubation layer body through the cooperation of the protrusion and the slide groove.

[0016] Furthermore, one side of the tray buckle is hinged to the slot-type tray, and the tray buckle is used to fix the PVDF membrane on the slot-type tray.

[0017] Furthermore, the slot tray has an L-shaped cross-section; one side of the slot tray with an L-shaped cross-section is connected to the incubation layer body, and a handle is provided on the other side, and the handle is used to pull the slot tray out of the incubation layer body.

[0018] Furthermore, the handle is designed with rubber to prevent liquid leakage.

[0019] Furthermore, the width of the slot tray is less than or equal to the width of the inner bottom surface of the incubation layer body; the length of the slot tray is less than or equal to the length of the inner bottom surface of the incubation layer body; an opening is provided at the bottom of the side surface of the incubation layer body; and the height of the slot tray is less than or equal to the height of the opening of the incubation layer body.

[0020] Further preferably, the length of the slot tray is equal to the length of the inner bottom surface of the incubation layer body, and the height of the slot tray is equal to the height of the opening of the incubation layer body.

[0021] Furthermore, when the slot tray is not pulled out, the size of one side of the handle of the slot tray with an L-shaped cross-section matches the size of the opening of the incubation layer body to ensure that the incubation layer body is in a relatively sealed environment.

[0022] Compared with the prior art, the utility model has the following beneficial effects:

[0023] 1) This technical solution provides an antibody incubation box for immunoblotting experiments. Different antibody diluents and membrane washing solutions can be injected and recovered through the inlet and outlet units (liquid inlet and outlet) on both sides, avoiding the mutual influence between heterogeneous antibodies. At the same time, there is no need to remove the PVDF membrane during the entire incubation process, which also reduces the risk of contamination caused by the external environment.

[0024] 2) The technical solution provides an antibody incubation box for immunoblotting experiments, which uses tray buckles on the slot-type tray to fix both ends of the PVDF membrane, thereby ensuring that the membrane will not shake randomly during the incubation process and the antibody can be evenly attached.

[0025] 3) The technical solution provides an antibody incubation box for immunoblotting experiments, which is equipped with multiple independent spaces to incubate and accommodate different strips. At the same time, a slot-type tray with a tray buckle is provided on each incubation layer to avoid uneven antibody incubation when the strips are immersed. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a schematic diagram of the structure of the antibody incubation box for immunoblotting experiments in Example 1 of the utility model.

[0027] Figure 2 It is a vertical cross-sectional schematic diagram of the antibody incubation box for immunoblotting experiment in Example 1 of the utility model.

[0028] Figure 3 It is a schematic transverse cross-sectional view of the antibody incubation box for immunoblotting experiments in Example 1 of the utility model.

[0029] Figure 4This is a schematic diagram of the connection between the slot tray and the incubation layer body of the antibody incubation box for immunoblotting experiments in Example 2 of the present utility model.

[0030] Figure 5 This is a schematic diagram of the connection between the slot-type tray and the tray buckle of the antibody incubation box for immunoblotting experiments in Example 1 of the utility model.

[0031] in:

[0032] 1. Box body; 2. Incubation layer; 3. Slot-type tray; 3-1. Protrusion; 3-2. Handle; 4. Tray buckle; 5. Liquid inlet; 6. Liquid outlet; 7. Sealing plug; 8. Incubation layer body; 8-1. Slide groove. DETAILED DESCRIPTION

[0033] The utility model is described in detail below in conjunction with the accompanying drawings and specific embodiments. The features such as component models, material names, connection structures, etc. that are not clearly described in this technical solution are all regarded as common technical features disclosed in the prior art.

[0034] In the present utility model, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral one; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements; "up", "down", "left", "right" and the like are only used to indicate relative positional relationships, and when the absolute position of the described object changes, the relative positional relationship may change. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0035] It should be noted that, in the present utility model, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0036] The utility model provides an antibody incubation box for immunoblotting experiments, comprising a box body, a multi-layer incubation layer and an internal slot-type tray. Each incubation layer is provided with two perfusion ports and a slot-type tray, wherein the left perfusion port is at a lower position and is responsible for liquid outflow, and the right perfusion port is at a higher position and is responsible for liquid inflow, and buckles are installed on both sides of the tray. When in use, the two ends of the PVDF membrane are fixed on the tray with the buckles. A primary antibody dilution is poured into the higher perfusion port on the right side, and the perfusion port is sealed with a rubber stopper for incubation. After the incubation is completed, the lower perfusion port on the left side is opened, and the primary antibody dilution flows out from the slot to recover the antibody. In the subsequent membrane washing and secondary antibody incubation process, the membrane washing solution and the secondary antibody dilution can also flow in and out from the perfusion ports on both sides.

[0037] The utility model sets up multiple independent spaces to incubate and accommodate different strips, and sets a tray with a buckle on each incubation layer to avoid uneven antibody incubation when soaking the strips. In addition, the setting of the perfusion ports on both sides makes the addition and recovery of each layer of solution non-interfering, avoiding mutual contamination between antibodies. At the same time, the entire incubation process of the antibody can be achieved without removing the PVDF membrane during operation, which also reduces the risk of contamination caused by the external environment.

[0038] The content of the utility model is further described in detail below in conjunction with specific embodiments.

[0039] Example 1

[0040] This embodiment provides an antibody incubation kit for immunoblotting experiments.

[0041] like Figures 1 to 3 As shown, the antibody incubation box includes an incubation layer 2; the incubation layer 2 includes an incubation layer body 8, a slot tray 3, a tray buckle 4, a liquid inlet 5, a liquid outlet 6 and a sealing plug 7; each incubation layer 2 is provided with two perfusion ports as liquid inlet and outlet units, the two perfusion ports are respectively the liquid inlet 5 and the liquid outlet 6, to ensure that each incubation layer has an independent liquid inlet and outlet unit, the liquid inlet 5 and the liquid outlet 6 are respectively opened on both sides of the incubation layer body 8; the slot tray 3 is connected to the incubation layer body 8 to carry the PVFD film; the tray buckle 4 is arranged on the slot tray 3 to fix the PVFD film; the sealing plug 7 is detachably connected to the liquid inlet 5 and the liquid outlet 6, the size of the sealing plug 7 matches the size of the liquid inlet 5 and the liquid outlet 6, the liquid inlet 5 and the liquid outlet 6 are both sealed by the sealing plug 7 to prevent internal liquid leakage during the incubation process.

[0042] The setting position of the liquid inlet 5 on the same incubation layer 2 is higher than the setting position of the liquid outlet 6, and the liquid inlet 5 and the liquid outlet 6 are staggered in front and back.

[0043] A plurality of incubation layers 2 are stacked to form the box body 1 , and each incubation layer 2 is configured identically.

[0044] The slot-type tray 3 is a tray made of rubber material.

[0045] The sealing plug 7 is a sealing plug made of rubber material.

[0046] The incubation layer body 8 is a rectangular parallelepiped shell.

[0047] like Figure 5 As shown, one side of the tray buckle 4 is hinged to the slot-type tray 3 , and the tray buckle 4 is used to fix the PVDF membrane on the slot-type tray 3 .

[0048] The cross-section of the slot tray 3 is L-shaped; a handle 3-2 is provided on one side of the slot tray 3 with an L-shaped cross-section, and the handle 3-2 is used to pull the slot tray 3 out of the incubation layer body 8, and the other side of the slot tray 3 with an L-shaped cross-section can be mounted on the bottom plate of the incubation layer body 8.

[0049] The handle 3-2 is designed with rubber to prevent liquid leakage.

[0050] The slot tray 3 with an L-shaped cross section is provided with a handle 3 - 2 with one side being a handle side and the other side being a bottom side, wherein the handle side is connected to the bottom side, wherein the height of the handle side is higher than the bottom side.

[0051] The width of the slot tray 3 is equal to the width of the inner bottom surface of the incubation layer main body 8; the length of the slot tray 3 is equal to the length of the inner bottom surface of the incubation layer main body 8, the height of the slot tray 3 at the handle is equal to the height of the opening of the incubation layer main body 8, and the height of the bottom edge is lower than the height of the opening of the incubation layer main body 8.

[0052] When the slot tray 3 is not pulled out, the slot tray 3 with an L-shaped cross section has a side with a handle 3 - 2 whose size matches the size of the opening of the incubation layer body 8 to ensure that the incubation layer body 8 is in a relatively sealed environment.

[0053] The working method of the above-mentioned antibody incubation kit for immunoblotting experiments is as follows:

[0054] Before the antibody is formally incubated, the sealed PVDF membrane is first cut into a suitable size. After the slot tray 3 is pulled out from the incubation layer body 8, the PVDF membrane is fixed to the slot tray 3 using the tray buckle 4. Then, the slot tray 3 is pushed back to its original position. The slot tray 3 with an L-shaped cross-section is provided with one side of the handle 3-2 aligned with the opening of the incubation layer body 8, so that the incubation layer body 8 is in a relatively sealed environment.

[0055] The antibody solution can be injected and recovered in a relatively sealed environment through the liquid inlet 5 and the liquid outlet 6. When injecting the antibody solution, the sealing plug 7 on the liquid inlet 5 is opened, and the solution can be injected from the liquid inlet 5 using a pipette. After injection, the sealing plug 7 is plugged back into the liquid inlet 5. When recovering the antibody, it is only necessary to open the sealing plug 7 at the liquid outlet 6, and the box body solution can slowly flow out from the liquid outlet 6 at a designated position by tilting the box body. After flowing out, the sealing plug 7 is plugged back into the liquid outlet 6. This process reduces mutual contamination between antibodies and reduces the contact between the PVDF membrane and the outside air, thereby reducing the impact on subsequent color development.

[0056] Example 2

[0057] This embodiment, based on Embodiment 1, further includes the following configuration:

[0058] like Figure 4 As shown, the bottom of the incubation layer body 8 is provided with a slide groove 8-1; the bottom of the slot tray 3 is provided with a protrusion 3-1; the protrusion 3-1 cooperates with the slide groove 8-1; the slot tray 3 is slidably connected to the incubation layer body 8 through the cooperation between the protrusion 3-1 and the slide groove 8-1. The bottom of the bottom edge of the slot tray 3 with an L-shaped cross section is provided with a protrusion 3-1, and is connected to the incubation layer body 8 through the protrusion 3-1.

[0059] The above description of the embodiments is to facilitate the understanding and use of the utility model by those skilled in the art. It is obvious that those familiar with the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the utility model is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the utility model without departing from the scope of the utility model should be within the scope of protection of the utility model.

Claims

1. An antibody incubation kit for immunoblotting experiments, characterized in that: The antibody incubation box comprises an incubation layer (2); The incubation layer (2) comprises an incubation layer body (8), a slot-type tray (3), a tray buckle (4), a liquid inlet (5), a liquid outlet (6) and a sealing plug (7); The liquid inlet (5) and the liquid outlet (6) are respectively arranged on two sides of the incubation layer body (8); The slot-type tray (3) is connected to the incubation layer body (8); The tray buckle (4) is arranged on the slot-type tray (3); The sealing plug (7) is detachably connected to the liquid inlet (5) and the liquid outlet (6).

2. An antibody incubation kit for immunoblotting experiments according to claim 1, characterized in that: The setting position of the liquid inlet (5) on the same incubation layer (2) is higher than the setting position of the liquid outlet (6).

3. An antibody incubation kit for immunoblotting experiments according to claim 1, characterized in that: A plurality of incubation layers (2) are stacked to form a box body (1).

4. The antibody incubation kit for immunoblotting experiments according to claim 1, characterized in that: The slot-type tray (3) is a tray made of rubber material.

5. The antibody incubation kit for immunoblotting experiments according to claim 1, characterized in that: The sealing plug (7) is a sealing plug made of rubber material.

6. The antibody incubation kit for immunoblotting experiments according to claim 1, characterized in that: The incubation layer body (8) is a rectangular parallelepiped shell.

7. The antibody incubation kit for immunoblotting experiments according to claim 1, characterized in that: A slide groove (8-1) is provided at the bottom of the incubation layer body (8); The bottom of the slot-type tray (3) is provided with a protrusion (3-1); The protrusion (3-1) cooperates with the slide groove (8-1); The slot-type tray (3) is slidably connected to the incubation layer body (8) through the cooperation of the protrusion (3-1) and the slide groove (8-1).

8. The antibody incubation kit for immunoblotting experiments according to claim 1, characterized in that: One side of the tray buckle (4) is hinged to the slot-type tray (3), and the tray buckle (4) is used to fix the PVDF membrane on the slot-type tray (3).

9. The antibody incubation kit for immunoblotting experiments according to claim 1, characterized in that: The cross section of the slot tray (3) is L-shaped; One side of the slot tray (3) with an L-shaped cross section is connected to the incubation layer body (8), and the other side is provided with a handle (3-2), wherein the handle (3-2) is used to pull the slot tray (3) out of the incubation layer body (8).

10. The antibody incubation kit for immunoblotting experiments according to claim 1, characterized in that: The width of the slot tray (3) is less than or equal to the width of the incubation layer body (8); The length of the slot tray (3) is less than or equal to the length of the incubation layer body (8); The bottom of the side of the incubation layer body (8) is provided with an opening; The height of the slot-type tray (3) is less than or equal to the height of the opening of the incubation layer body (8).

Images