Detection disc with solid-liquid separation device and chemical immunoluminescence instrument thereof

Abstract

The utility model relates to a detection disc with a solid-liquid separation device, the detection disc comprises a PMT assembly, a driving mechanism and a detection rotating disc, the detection rotating disc comprises a rotating disc body and a shading upper cover covering the rotating disc body, the rotating disc body is internally provided with a plurality of accommodating cavities, and the shading upper cover is provided with an inlet and outlet pipe opening, a plurality of liquid filling openings and a liquid suction opening; a liquid suction device is arranged above the liquid suction port and comprises a first fixed seat fixed on the upper surface of the shading upper cover, a support vertically arranged on the first fixed seat, a sliding block movably connected with the support and provided with a cantilever beam, and a liquid suction needle vertically arranged on the cantilever beam, the liquid suction end of the liquid suction needle is close to the liquid suction port, and the liquid outlet end of the liquid suction needle is close to the cantilever beam; and the liquid outlet pipe is connected with the suction pump. By arranging the liquid suction needles on the detection disc, waste liquid in the reaction tube is sucked away, the solid-liquid separation effect is achieved, the problems of waste liquid scattering and leakage and the like caused by solid-liquid mixing are avoided, the risk that the external environment is polluted by the waste liquid is reduced, and the safety of the detection link is improved.

Description

Technical Field

[0001] The present application relates to the technical field of immunoassay equipment, and in particular to a detection plate with a solid-liquid separation device and a chemiimmunoluminometer thereof. Background Art

[0002] Chemiimmunoluminescence (CISS) is a commonly used bioanalytical technology that is widely used in biochemistry, molecular biology, clinical medicine and other fields. The test plate in the chemiimmunoluminescence instrument is an important device used to fix and detect in vitro biological samples. When using the test plate to detect the reaction solution, the biological sample (such as protein, DNA, cells, etc.) is added to the appropriate position of the test plate, and appropriate reagents such as the excitation solution are added when necessary. The CISS instrument detects and quantifies biological samples through chemical immunoreactions and luminescence technology. The test plate can fix and protect biological samples, and can detect multiple samples at the same time, improving detection efficiency and effectiveness.

[0003] However, after the test is completed, the mixed liquid containing the biological sample is usually discarded together with the reaction tube. After the test is completed, the reaction tube may contain liquids with strong biological hazards. If the reaction tube and the internal liquid are not separated and discarded together, it will not only be difficult to transport the waste, but there may also be a high risk of leakage due to the mixing of solid and liquid phases, which may cause harm to the external environment. Utility Model Content

[0004] In view of the technical problems existing in the prior art, the present application proposes a test plate with a solid-liquid separation device and a chemiimmunoluminometer thereof. By arranging a liquid suction needle on the test plate to achieve the effect of solid-liquid separation, the problem of solid-liquid mixing and waste liquid leaking to the outside can be avoided, the risk of waste liquid contaminating the external environment can be reduced, and the safety of the detection process can be improved. The present application proposes a detection disk with a solid-liquid separation device, including a PMT component, a driving mechanism and a detection turntable, the detection turntable including a turntable body and a light-shielding upper cover arranged on the turntable body, the turntable body is provided with a plurality of accommodating chambers for accommodating reaction tubes, the light-shielding upper cover is respectively provided with inlet and outlet pipes, a plurality of liquid filling ports and a liquid suction port; a liquid suction device is provided above the liquid suction port, the liquid suction device includes a first fixed seat fixed to the upper surface of the light-shielding upper cover, a bracket vertically arranged on the first fixed seat, a slider provided with a cantilever beam movably connected to the bracket, and a liquid suction needle vertically arranged on the cantilever beam, a hollow liquid suction chamber is provided inside the liquid suction needle, the liquid suction end thereof is close to the liquid suction port, and the liquid outlet end thereof is close to the cantilever beam, and is connected to a suction pump through a liquid outlet pipe.

[0005] Optionally, according to an embodiment of the present application, a through hole for placing a first guide sleeve is opened on the cantilever beam, and the pipette needle moves up and down along the pores in the first guide sleeve body, and the first guide sleeve is fixed to the cantilever beam through a transverse mounting plate.

[0006] Optionally, according to an embodiment of the present application, the liquid outlet end is connected to the liquid outlet tube through a cylindrical or boss-shaped first limiting member; a through hole is opened inside the first limiting member and is tightly mounted on the side wall of the liquid pipette needle between the liquid outlet end and the cantilever beam; the liquid outlet tube tightly wraps around the cylindrical outer wall of the first limiting member or the outer wall of the end with a smaller boss diameter, and the diameter of the cylindrical first limiting member or the diameter of the boss-shaped first limiting member on the side with a larger diameter is larger than the pores of the first guide sleeve.

[0007] Optionally, according to an embodiment of the present application, an elastic component is provided below the cantilever beam and is sleeved outside the pipetting needle. The elastic component includes a fixed end fixed to the outer wall of the first guide sleeve or the cantilever beam, a spring body and a free end, and the free end is fixedly connected to the side wall of the pipetting needle.

[0008] Optionally, according to an embodiment of the present application, the elastic member is fixedly connected to the pipette needle through a second limiting member, the outer side of the second limiting member is in the shape of a boss, the side with a smaller diameter faces the free end and is adapted to the diameter of the free end of the elastic member, and the free end is fixedly sleeved on the outer wall of the side with a smaller diameter of the boss; a through hole is opened in the second limiting member to cooperate with the outer wall of the pipette needle and is tightly sleeved on the side wall of the pipette needle.

[0009] Optionally, according to an embodiment of the present application, the first fixing seat is provided with a through hole for placing the second guide sleeve, and the pipette needle can move freely up and down along the pores in the second guide sleeve body.

[0010] Optionally, according to an embodiment of the present application, the second guide sleeve is a T-shaped sleeve, which is fixed to the first fixing seat by bolts.

[0011] Optionally, according to an embodiment of the present application, the second guide sleeve is interference fit with the liquid suction port, and the through hole of the first guide sleeve, the through hole of the second guide sleeve and the liquid suction port are all coaxially arranged.

[0012] Optionally, according to an embodiment of the present application, the driving mechanism can drive the turntable body to rotate, so that the accommodating chamber moves to a coaxial position below the pipe inlet, the plurality of liquid filling ports or the liquid suction port.

[0013] Optionally, according to an embodiment of the present application, a clamp is correspondingly provided above the inlet and outlet pipe opening, and the clamp places the reaction tube to be tested into the inlet and outlet pipe opening, and takes out the waste tube after the reaction liquid has been sucked away by the liquid suction device from the inlet and outlet pipe opening and places it into a garbage disposal device.

[0014] The present application also proposes a chemiimmunoluminometer, which comprises at least a sample library and a reagent compartment, and is characterized in that it also comprises a detection disk with a solid-liquid separation device as described in any of the above items, wherein the detection turntable is used to detect antigens or antibodies in the reaction liquid placed in the reaction tube.

[0015] The present application achieves the effect of solid-liquid separation by arranging a liquid suction needle on the detection plate, thereby avoiding the problem of solid and liquid mixing together and causing waste liquid to leak to the outside. This can reduce the risk of waste liquid contaminating the external environment and improve the safety of the detection process. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Below, the preferred embodiments of the present application will be further described in detail with reference to the accompanying drawings, wherein:

[0017] Figure 1 This is a schematic diagram of the overall structure of a detection disk with a solid-liquid separation device according to an embodiment of the present application;

[0018] Figure 2 yes Figure 1 A-direction view;

[0019] Figure 3 yes Figure 2 BB section of ; and

[0020] Figure 4 yes Figure 3 Cross-sectional view along the axial direction of the pipette needle at point C.

[0021] Figure numerals: PMT assembly 100; driving mechanism 200; detection turntable 500; turntable body 510; accommodating chamber 511; light-shielding upper cover 520; inlet and outlet pipes 521; liquid filling port 522; liquid aspiration device 700; first fixed seat 710; bracket 715; slider 720; cantilever beam 725; liquid aspiration needle 770; liquid outlet end 772; first guide sleeve 730; horizontal mounting plate 731; second guide sleeve 735; first limit member 740; elastic member 750; second limit member 760. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solution and advantages of the embodiments of the present application clearer, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0023] In the following detailed description, reference may be made to the various specification drawings that are part of the present application and are used to illustrate specific embodiments of the present application. In the accompanying drawings, similar reference numerals describe substantially similar components in different figures. The various specific embodiments of the present application are described below in sufficient detail so that a person of ordinary skill in the art with relevant knowledge and skills in the art can implement the technical solutions of the present application. It should be understood that other embodiments may also be used or structural, logical or electrical changes may be made to the embodiments of the present application.

[0024] like Figure 1-Figure 3 As shown, the embodiment of the present application proposes a detection disk with a solid-liquid separation device, including a PMT assembly 100, a driving mechanism 200 and a detection turntable 500, the detection turntable 500 includes a turntable body 510 and a light-shielding upper cover 520 covered on the turntable body, a plurality of accommodating chambers 511 for accommodating reaction tubes are arranged in the turntable body 510, and the light-shielding upper cover 520 is respectively provided with an inlet and outlet pipe opening 521, a plurality of liquid filling ports 522 and a liquid suction port (not shown in the figure). The PMT assembly 1 is fixed to the outer side of the turntable body 510 by a PMT fixing seat (not shown in the figure). Because the photomultiplier tube (PMT for short) is used in the detection disk of the chemical immunoassay analyzer to detect the luminous intensity, and the PMT assembly 1 needs to be in a strictly light-proof environment when performing the detection, if light leakage occurs, it will affect the accuracy of the detected light value data. Therefore, in order to avoid light leakage in the embodiment of the present application, a light shielding cover 520 is used to cover the turntable body 510 to form a light-proof accommodating chamber 511. Figure 1 As shown, in some embodiments, the reaction tube is placed into the containing chamber 511 of the detection disk through the inlet and outlet pipe port 521, rotated clockwise to the bottom of the filling port 522, and after one or more excitation liquids are filled, it is rotated clockwise to the bottom of the suction port.

[0025] Continue as Figure 1-Figure 3As shown, a liquid suction device 700 is provided above the liquid suction port, and the liquid suction device 700 includes a first fixed seat 710 fixed on the upper surface of the light-shielding upper cover 520, a bracket 715 vertically arranged on the first fixed seat 710, a slider 720 movably connected to the bracket 715 and provided with a cantilever beam 725, and a liquid suction needle 770 vertically arranged on the cantilever beam 725, and the liquid suction needle 770 is provided with a hollow liquid suction chamber (not shown in the figure) inside, and its liquid suction end (not shown in the figure) is close to the liquid suction port, and its liquid outlet end 772 is close to the cantilever beam 725, and is connected to a suction pump (not shown in the figure) through a liquid outlet pipe (not shown in the figure). When the reaction tube after detection rotates to the position of the liquid aspiration port, the upper liquid aspiration needle 770 moves downward under the drive of the slider 720, and the liquid aspiration end extends into the tube body. The waste liquid in the reaction tube is sucked out under the suction action of the external suction pump, and the waste liquid enters the liquid outlet tube connected to the liquid outlet end 772 of the liquid aspiration needle through the hollow liquid aspiration chamber of the liquid aspiration needle 770 and is drained out.

[0026] Therefore, the present application achieves the effect of separating the reaction waste liquid from the reaction tube by arranging a liquid suction needle on the detection tray, avoiding the problem of solid-liquid mixing to cause waste liquid to leak to the outside, which is not only convenient for the transportation and transfer of medical waste, but also effectively reduces the risk of waste liquid polluting the external environment and improves the safety of the detection link. In some embodiments, a container with stronger sealing and thicker material can be used for the transfer device containing waste liquid to prevent the waste liquid from leaking due to being punctured by external sharp objects, and a lighter packaging material can be used for the reaction tube. Therefore, the detection tray with a solid-liquid separation device of the present application is convenient for the subsequent transportation of solids and liquids separately, which can effectively reduce the total cost of transporting waste materials.

[0027] like Figure 1-Figure 4 As shown, in some embodiments, a through hole (not shown) for placing the first guide sleeve 730 is opened on the cantilever beam 725, and the liquid suction needle 770 moves up and down along the pores in the sleeve body of the first guide sleeve 730, and the first guide sleeve 730 is fixed on the cantilever beam 725 through a transverse mounting plate 731. The first guide sleeve 730 extends a certain length in the axial direction, and the movement of the liquid suction needle 770 in the sleeve body can ensure the stability of the movement, and the sleeve body plays an accurate guiding role for the liquid suction needle.

[0028] like Figure 1-Figure 4As shown, the liquid outlet 772 is connected to the liquid outlet pipe through a cylindrical or boss-shaped first stopper 740; a through hole is provided inside the first stopper 740 and is tightly sleeved on the side wall of the liquid aspiration needle between the liquid outlet 772 and the cantilever beam 725; the liquid outlet pipe tightly wraps the cylindrical outer wall of the first stopper 740 or the outer wall of the end with a smaller diameter of the boss. When the first stopper 740 is cylindrical, the diameter of the first stopper 740 is larger than the pore of the first guide sleeve; when the first stopper 740 is boss-shaped, the diameter of the larger diameter side of the first stopper 740 is larger than the pore of the first guide sleeve, in other words, the first stopper 740 is located above the first guide sleeve 731 and will not fall off. By installing the first stopper 740 tightly connected to the outer wall of the liquid aspiration needle 770, the liquid aspiration needle 770 can be prevented from falling from the first guide sleeve 730, and it is also convenient for the liquid outlet pipe to be connected to the liquid aspiration needle 770.

[0029] like Figure 1-Figure 4 As shown, optionally, according to an embodiment of the present application, an elastic member 750 is provided below the cantilever beam 725, which is sleeved outside the liquid-absorbing needle 770, and the elastic member 750 includes a fixed end (not shown) fixed to the outer wall of the first guide sleeve 730 or the cantilever beam 725, a spring body (not shown) and a free end (not shown), and its free end is fixedly connected to the side wall of the liquid-absorbing needle 770. When the liquid-absorbing end falls to the bottom of the contact reaction tube, under the elastic buffering effect of the elastic member 750, it will touch the bottom flexibly without damaging the liquid-absorbing needle. When it contacts the bottom, the liquid-absorbing needle 770 moves upward along the first guide sleeve 731 and drives the first stopper 740 closely connected thereto to move upward, intuitively reflecting that the liquid-absorbing needle has contacted the bottom of the reaction tube, and the waste liquid in the reaction tube has been completely sucked away, without residual liquid. The reason for this design is that only when the liquid-absorbing end contacts the bottom of the reaction tube can the waste liquid in the tube be completely sucked away. However, if the aspiration needle 770 cannot move upward during the downward movement, it may be damaged due to the rigid contact with the bottom of the tube, and the reaction tube may also be damaged due to the excessive impact force of the needle; however, if there is no resistance when moving upward, it is difficult to ensure that the aspiration end 772 stably contacts the bottom of the tube, making it difficult to absorb the waste liquid at the bottom of the tube. Therefore, the provision of a flexible bottom-touching device can not only protect the aspiration needle and the reaction tube, but also help the aspiration needle to suck the waste liquid at the bottom of the tube cleanly.

[0030] like Figure 1-Figure 4As shown, optionally, according to an embodiment of the present application, the elastic member 750 is fixedly connected to the liquid suction needle 770 through the second stopper 760, the outer side of the second stopper 760 is in the shape of a boss, the side with a smaller diameter faces the free end and is adapted to the diameter of the free end of the elastic member, and the free end is fixedly sleeved on the outer wall of the side with a smaller diameter of the boss; a through hole is provided in the second stopper 760 to match the outer wall of the liquid suction needle 770 and is tightly sleeved on the side wall of the liquid suction needle 770. When the liquid suction needle 770 touches the bottom of the tube, it is subjected to an upward force and moves upward with the liquid suction needle, squeezing the elastic member 750 to achieve flexible bottoming. By adding the second stopper 760, it is convenient for the free end of the elastic member 750 to be fixedly connected to the outer wall of the liquid suction needle 770.

[0031] like Figure 1-Figure 4 As shown, optionally, according to an embodiment of the present application, the first fixed seat 710 is provided with a through hole (not shown) for placing the second guide sleeve 735, and the liquid suction needle 770 moves freely up and down along the pores in the second guide sleeve 735 sleeve body. The second guide sleeve 735 is close to the liquid suction end 772, and the first guide sleeve 730 is close to the liquid outlet end 772, and the second guide sleeve 735 also plays a guiding role. When the liquid suction needle 770 is in the initial position, the liquid suction end 772 can be always controlled below the second guide sleeve 735 by adjusting the total length of the liquid suction needle 770 or the height of the slider 720 lifting. Under the action of the second guide sleeve 735, the liquid suction end 772 will not be difficult to accurately enter the liquid suction port because of shaking. In order to facilitate fixed installation, in some embodiments, the second guide sleeve 735 is a T-shaped sleeve body, which is fixed on the first fixed seat 710 by bolts.

[0032] Further, as before, the PMT assembly 1 needs to be in a strictly light-proof environment when performing detection. If light leakage occurs, the accuracy of the detected light value data will be affected. The second guide sleeve 735 and the liquid-absorbing needle 770 that is only telescopic inside the sleeve body block the external light source, which can play a good light-proof role.

[0033] like Figure 4 As shown, optionally, according to an embodiment of the present application, the second guide sleeve 735 is interference fit with the liquid suction port, and the through hole of the first guide sleeve 730, the through hole of the second guide sleeve 735 and the liquid suction port are all coaxially arranged. Coaxiality ensures that the liquid suction needle 770 can move vertically up and down along the two guide sleeves in the field.

[0034] Optionally, according to an embodiment of the present application, the driving mechanism 200 can drive the turntable body 510 to rotate, so that the accommodating chamber 511 moves to the coaxial position below the inlet and outlet pipe 521, the multiple filling ports 522 or the aspiration port. This can also ensure that the aspiration needle 770 can accurately extend into the bottom of the reaction tube located in the accommodating chamber 511 to absorb the waste liquid cleanly.

[0035] In some embodiments, a clamp is provided above the inlet and outlet pipe opening 521. The clamp puts the reaction tube to be tested into the inlet and outlet pipe opening, and takes out the waste tube after the reaction liquid has been sucked away by the liquid suction device from the inlet and outlet pipe opening and puts it into the garbage disposal device. The clamp can complete both tube placement and tube removal. There is no waste liquid in the taken out waste tube, which is convenient for collection and transportation.

[0036] The present application also proposes a chemiimmunoluminometer, which at least includes a sample library and a reagent compartment and also includes a detection disk with a solid-liquid separation device as described above. The detection turntable 500 is used to detect antigens or antibodies in the reaction solution placed in the reaction tube.

[0037] The detection tray with a solid-liquid separation device of the present application achieves the effect of separating the reaction waste liquid from the reaction tube by arranging a liquid suction needle on the detection tray, avoiding the problem of solid-liquid mixing and causing waste liquid to leak to the outside. It is not only convenient for the subsequent transportation of solids and liquids separately, but also can effectively reduce the total cost of transporting waste materials, and effectively reduce the risk of waste liquid polluting the external environment, thereby improving the safety of the detection process. While covering the opening with a light-shielding cover, the requirement of light protection is also taken into account, preventing external light sources from entering the interior of the accommodating chamber through the opening, thereby ensuring the accuracy of the detection results.

[0038] The above embodiments are only used to illustrate the present application, and are not intended to limit the present application. Ordinary technicians in the relevant technical field may make various changes and modifications without departing from the scope of the present application. Therefore, all equivalent technical solutions should also fall within the scope disclosed in the present application.

Claims

1. A detection disk with a solid-liquid separation device, comprising a PMT assembly, a driving mechanism and a detection turntable, wherein the detection turntable comprises a turntable body and a light-shielding upper cover arranged on the turntable body, wherein a plurality of accommodating chambers for accommodating reaction tubes are arranged in the turntable body, and the light-shielding upper cover is respectively provided with inlet and outlet ports, a plurality of liquid filling ports and a liquid suction port; characterized in that: A liquid suction device is provided above the liquid suction port, and the liquid suction device includes a first fixed seat fixed to the upper surface of the light-shielding upper cover, a bracket vertically arranged on the first fixed seat, a slider movably connected to the bracket and provided with a cantilever beam, and a liquid suction needle vertically arranged on the cantilever beam, wherein a hollow liquid suction chamber is provided inside the liquid suction needle, wherein the liquid suction end thereof is close to the liquid suction port, and the liquid outlet end thereof is close to the cantilever beam, and is connected to a suction pump via a liquid outlet pipe.

2. The detection plate with a solid-liquid separation device according to claim 1, characterized in that: The cantilever beam is provided with a through hole for placing the first guide sleeve, the liquid pipetting needle moves up and down along the pores in the first guide sleeve body, and the first guide sleeve is fixed on the cantilever beam through a transverse mounting plate.

3. The detection plate with a solid-liquid separation device according to claim 2, characterized in that: The liquid outlet end is connected to the liquid outlet tube through a cylindrical or boss-shaped first limiting member; a through hole is opened inside the first limiting member and is tightly mounted on the side wall of the liquid aspiration needle between the liquid outlet end and the cantilever beam; the liquid outlet tube tightly wraps the cylindrical outer wall of the first limiting member or the outer wall of the end with a smaller boss diameter, and the diameter of the cylindrical first limiting member or the diameter of the boss-shaped first limiting member with a larger diameter is larger than the pores of the first guide sleeve.

4. The detection plate with a solid-liquid separation device according to claim 2, characterized in that: An elastic component is provided below the cantilever beam and is sleeved outside the pipetting needle. The elastic component includes a fixed end fixed to the outer wall of the first guide sleeve or the cantilever beam, a spring body and a free end, and the free end is fixedly connected to the side wall of the pipetting needle.

5. The detection plate with a solid-liquid separation device according to claim 4, characterized in that: The elastic member is fixedly connected to the pipette needle through a second limiting member, the outer side of the second limiting member is in the shape of a boss, the side with a smaller diameter faces the free end and is adapted to the diameter of the free end of the elastic member, and the free end is fixedly sleeved on the outer wall of the side with a smaller diameter of the boss; a through hole is opened in the second limiting member to cooperate with the outer wall of the pipette needle and is tightly sleeved on the side wall of the pipette needle.

6. The detection plate with a solid-liquid separation device according to claim 2, characterized in that: The first fixing seat is provided with a through hole for placing the second guide sleeve, and the liquid-pipetting needle can freely move up and down along the pores in the second guide sleeve.

7. The detection plate with a solid-liquid separation device according to claim 6, characterized in that: The second guide sleeve is a T-shaped sleeve body, which is fixed on the first fixing seat by bolts.

8. The detection plate with a solid-liquid separation device according to claim 6, characterized in that: The second guide sleeve is interference fit with the liquid suction port, and the through hole of the first guide sleeve, the through hole of the second guide sleeve and the liquid suction port are all coaxially arranged.

9. The detection plate with a solid-liquid separation device according to claim 1, characterized in that: The driving mechanism can drive the turntable body to rotate, so that the accommodating chamber moves to the pipe inlet, the plurality of liquid filling ports or a coaxial position below the liquid suction port.

10. The detection plate with a solid-liquid separation device according to claim 1, characterized in that: A clamp is provided above the inlet and outlet pipe opening, and the clamp puts the reaction tube to be tested into the inlet and outlet pipe opening, and takes out the waste tube after the reaction liquid has been sucked away by the liquid suction device from the inlet and outlet pipe opening and puts it into the garbage disposal device.

11. A chemiimmunoluminometer, comprising at least a sample library and a reagent compartment, characterized in that: It also includes a detection disk with a solid-liquid separation device as described in any one of claims 1 to 10, wherein the detection turntable is used to detect antigens or antibodies in the reaction solution placed in the reaction tube.

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