Closed continuous column chromatography device

Abstract

The utility model relates to the field of biological fermentation, in particular to a closed continuous column chromatography device. According to the closed continuous column chromatography device, the design of the connectable multi-section chromatographic column is adopted, and the problem that the efficiency is low when a traditional single chromatographic column is used for large-scale or continuous separation is solved. By means of the design, long-time operation and treatment of a large number of samples are allowed, the separation efficiency is remarkably improved, and the time needed by the separation process is shortened. By adopting the innovative design that the elastic metal sealing groove is matched with the convex ring, the problem that the sealing performance of an existing connecting mode is poor is solved. By means of the design, under the high-pressure condition or when a volatile solvent is used, sample leakage and pollution can be effectively prevented, and the stability and reliability of the separation effect are guaranteed.

Description

Technical Field

[0001] The utility model relates to the field of biological fermentation, and particularly relates to a closed continuous column chromatography device. Background Art

[0002] Column chromatography is a widely used technique in the fields of chemical analysis and separation. It utilizes the differences in the distribution coefficients of different substances between the stationary phase and the mobile phase to achieve separation. Traditional column chromatography devices usually consist of a single chromatographic column, a sample injection device, an eluent reservoir, a detector, and other components. However, with the continuous improvement of scientific research and industrial requirements, traditional column chromatography devices have become difficult to meet the actual needs in some aspects.

[0003] Firstly, traditional column chromatography devices usually use a single chromatographic column, which has low efficiency when performing large-scale or continuous separation. For cases that require long-term operation or processing of a large number of samples, the design of a single chromatographic column often fails to meet the requirements, resulting in a long separation process and low efficiency.

[0004] Secondly, there are many problems with the connection methods between existing chromatographic columns. Common connection methods include threaded connection, snap connection, etc. However, these connection methods often have disadvantages such as poor sealing performance and inconvenient operation. In the case of high pressure or the use of volatile solvents, poor sealing may lead to sample leakage, contamination, or a reduction in separation effect.

[0005] In view of the above problems, there is an urgent need for a closed continuous column chromatography device that can achieve efficient continuous separation, has excellent sealing performance, is convenient to operate, and has a perfect heating and stirring function. Summary of the Utility Model

[0006] To solve the above problems, the utility model discloses a closed continuous column chromatography device.

[0007] To achieve the above object, the present invention provides a closed continuous column chromatography device, including a reflux condenser, a chromatographic column, and a flask. Among them, the reflux condenser is connected to the upper end of the chromatographic column, the flask is connected to the lower end of the chromatographic column, a magnetic stirrer is provided at the bottom end of the flask, the chromatographic column includes a column body, one end of the column body is provided with an elastic metal sealing groove, the other end of the column body is provided with a convex ring for mating with the elastic metal sealing groove in position. When adjacent two sections of chromatographic columns are connected and mated, the convex ring of one section of the chromatographic column is inserted into the elastic metal sealing groove of the other section, and the two side walls of the elastic metal sealing groove abut against the convex ring to form a seal, and the other end of the column body is sealed by a flange.

[0008] Furthermore, the inner edge of one end of the column body is provided with an inwardly concave first sealing inclined surface, and the inner side of one end of the column body protrudes with a second sealing inclined surface for mating with the first sealing inclined surface in position.

[0009] In addition, when two adjacent flanges are connected in alignment, one of the two flanges is provided with a sealing ring, and the other is provided with a pressing ring for pressing the sealing ring in alignment.

[0010] The elastic metal sealing groove includes an annular base fixed to the end of the column; two elastic metal arms extending inward from the inner edge of the annular base to form a U-shaped structure.

[0011] The two side surfaces of the convex ring are inclined and gradually narrow from the top down to form a trapezoidal profile.

[0012] The closed continuous column chromatography device of the present invention adopts a design of multi-section chromatography columns that can be connected, overcoming the problem of low efficiency of traditional single chromatography columns in large-scale or continuous separation. This design allows for long-term operation and processing of a large number of samples, significantly improving the separation efficiency and shortening the time required for the separation process.

[0013] Excellent sealing performance: The present invention adopts an innovative design of the cooperation between an elastic metal sealing groove and a convex ring, solving the problem of poor sealing performance of the existing connection method. This design can effectively prevent sample leakage and contamination under high-pressure conditions or when using volatile solvents, ensuring the stability and reliability of the separation effect.

[0014] Operational convenience: Compared with traditional threaded connections or snap connections, the chromatography column connection method of the present invention is simpler and more intuitive. Operators can easily and quickly connect or disassemble the chromatography column, greatly improving the use efficiency and flexibility of the device. Description of the Drawings

[0015] Figure 1 It is a schematic diagram of the overall structure of the chromatography device in the embodiment of the present application;

[0016] Figure 2 It is a schematic diagram of the structure of the chromatography column in the embodiment of the present application;

[0017] Figure 3 It is a schematic diagram of the connection and cooperation of two chromatography columns in the embodiment of the present application;

[0018] Figure 4 In the embodiment of the present application Figure 3 The partial enlarged schematic diagram at A;

[0019] Figure 5 It is a schematic diagram of the position of the first sealing inclined surface in the embodiment of the present application;

[0020] Figure 6 It is a schematic diagram of the position of the second sealing inclined surface in the embodiment of the present application. Detailed Description of the Invention

[0021] The following further elaborates on the present utility model in conjunction with specific embodiments. It should be understood that the following specific embodiments are only used to illustrate the present utility model and not to limit the scope of the present utility model. It should be noted that the terms "front", "rear", "left", "right", "upper" and "lower" used in the following description refer to the directions in the drawings, and the terms "inner" and "outer" respectively refer to the directions towards or away from the geometric center of a specific component.

[0022] Embodiment 1: As Figures 1-6 shown, a closed continuous column chromatography device includes a reflux condenser 1, a chromatography column 2 and a flask 3. Among them, the reflux condenser 1 is connected to the upper end of the chromatography column 2, the flask 3 is connected to the lower end of the chromatography column 2, and a magnetic stirrer is provided at the bottom end of the flask 3. The chromatography column 2 includes a column body 11, and a ring-shaped elastic metal sealing groove 12 is provided at one end of the column body 11, and a convex ring 121 for mating with the elastic metal sealing groove 12 in alignment is provided at the other end of the column body 11 in alignment. When connecting and mating two adjacent sections of the chromatography column 2, the convex ring 121 of one section of the chromatography column 2 is inserted into the elastic metal sealing groove 12 of the other section, and the two side walls of the elastic metal sealing groove 12 abut against the convex ring 121 to form a seal. The other end of the column body 11 is sealed by a flange 13.

[0023] In this embodiment, the chromatography column 2 is the core part of the device and includes the following structures:

[0024] Column body 11: The main body part of the chromatography column.

[0025] Elastic metal sealing groove 12: Provided at one end of the column body 11 and is annular.

[0026] Convex ring 121: Provided at the other end of the column body 11 and corresponding to the elastic metal sealing groove 12 for mating.

[0027] Connection method of the chromatography column 2:

[0028] When it is necessary to connect two sections of the chromatography column 2, the following method is adopted:

[0029] The convex ring 121 of one section of the chromatography column 2 is inserted into the elastic metal sealing groove 12 of the other section of the chromatography column 2.

[0030] The two side walls of the elastic metal sealing groove 12 will closely abut against the convex ring 121 to form a seal.

[0031] Sealing of the column body 11:

[0032] The other end of the column body 11 is sealed by a flange 13 to ensure the tightness of the entire system.

[0033] Working principle:

[0034] The sample and solvent mixture are heated and evaporated from the flask 3.

[0035] Steam rises through the chromatography column 2 and is separated inside the column.

[0036] The separated components continue to rise to the reflux condenser 1.

[0037] After being condensed in the reflux condenser 1, the liquid flows down along the column wall, repeating the above process.

[0038] Advantages:

[0039] Good sealing performance: Through the cooperation of the elastic metal sealing groove 12 and the convex ring 121, a good sealing effect is achieved.

[0040] Modular design: Multiple chromatography columns can be connected as needed to enhance the separation effect.

[0041] Continuous operation: The reflux design enables the separation process to be carried out continuously, improving the efficiency.

[0042] In a specific solution, the elastic metal sealing groove 12 includes an annular base fixed at the end of the column body 11; two elastic metal arms that extend inward from the inner edge of the annular base to form a U-shaped structure.

[0043] When the convex ring 121 is inserted into the U-shaped structure, the two elastic metal arms will slightly separate.

[0044] The elastic force of the elastic metal arms will make them closely adhere to the surface of the convex ring 121.

[0045] This close contact ensures an excellent sealing effect.

[0046] Advantages:

[0047] Efficient sealing: The U-shaped structure provides a double sealing surface, enhancing the sealing effect.

[0048] Elastic adaptation: The elastic metal arms can adapt to minor dimensional changes to ensure the sealing performance during long-term use.

[0049] Strong durability: The use of metal materials increases the durability and corrosion resistance of the sealing structure.

[0050] Easy to load and unload: The U-shaped structure design makes the connection and disconnection processes simple and fast.

[0051] Self-adaptability: The elastic structure can adapt to the thermal expansion and contraction caused by temperature changes to maintain a stable seal.

[0052] The two sides of the convex ring 121 are inclined and gradually narrow from top to bottom, forming a trapezoidal profile.

[0053] When the convex ring 121 is inserted into the elastic metal sealing groove 12, the wider part of the top of the trapezoid contacts first. As the insertion depth increases, the inclined side of the convex ring 121 gradually presses the elastic metal arm. The trapezoidal design increases the contact area and improves the sealing effect. As the insertion depth increases, the sealing pressure gradually increases, achieving self-regulation.

[0054] Example 2: Figure 5 As shown, further, an inner edge of one end of the column 11 is provided with a concave first sealing bevel 14 , and an inner side of one end of the column 11 is provided with a convex second sealing bevel 141 which is aligned with the first sealing bevel 14 .

[0055] On the basis of Example 1, this embodiment optimizes the end structure of the column 11 and adds an additional sealing design. The details are as follows:

[0056] Column 11 end structure:

[0057] The first concave sealing slope 14 is arranged on the inner edge of one end of the column 11 .

[0058] The second sealing inclined surface 141 is convexly provided, and is located on the inner side of one end of the column 11 , and is aligned with the first sealing inclined surface 14 .

[0059] The working principle of the sealing bevel:

[0060] Double sealing: The first sealing bevel 14 and the second sealing bevel 141 form a wedge-shaped sealing structure.

[0061] Pressure distribution: When two inclined surfaces come into contact, the pressure is evenly distributed along the inclined surfaces, improving the sealing effect.

[0062] Example 3: Figure 4 As shown, in addition, when two adjacent flanges 13 are aligned and connected, one of the two flanges 13 is provided with a sealing ring 15, and the other flange is aligned with a pressing ring 151 for pressing the sealing ring 15. When the two flanges 13 are aligned and connected, the pressing ring 151 will press the sealing ring 15.

[0063] Deformation adaptation: The seal ring 15 deforms under pressure, fills the tiny gap and forms an effective seal. Combining the sealing structures in Examples 1 and 2, a comprehensive multi-layer sealing system is formed. The elastic metal seal groove 12 provides dynamic adaptability, the bevel seal provides static sealing, and the seal ring 15 provides a replaceable flexible seal.

[0064] The technical means disclosed by the solution of the present utility model are not limited to the technical means disclosed in the above embodiments, but also include technical solutions composed of any combination of the above technical features. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present utility model, several improvements and refinements can be made, and these improvements and refinements are also regarded as the protection scope of the present utility model.

Claims

1. A closed continuous column chromatography device, comprising a reflux condenser (1), a chromatography column (2) and a flask (3), wherein: The reflux condenser (1) is connected to the upper end of the chromatographic column (2), and the flask (3) is connected to the lower end of the chromatographic column (2). The bottom end of the flask (3) is provided with a magnetic stirrer, and is characterized in that the chromatographic column (2) comprises a column (11), one end of the column (11) is provided with a circle of elastic metal sealing groove (12), and the other end of the column (11) is provided with a convex ring (121) for aligning with the elastic metal sealing groove (12). When two adjacent sections of the chromatographic column (2) are connected and matched, the convex ring (121) of one section of the chromatographic column (2) is inserted into the elastic metal sealing groove (12) of the other section, and the two side walls of the elastic metal sealing groove (12) are against the convex ring (121) to form a seal; and the other end of the column (11) is sealed by a flange (13).

2. The closed continuous column chromatography device according to claim 1, characterized in that: An inner edge of one end of the column (11) is provided with a concave first sealing bevel (14), and an inner side of one end of the column (11) is provided with a convex second sealing bevel (141) aligned with the first sealing bevel (14).

3. The closed continuous column chromatography device according to claim 2, characterized in that: When two adjacent flanges (13) are aligned and connected, one of the two flanges (13) is provided with a sealing ring (15), and the other flange is aligned with a pressing ring (151) for pressing the sealing ring (15).

4. The closed continuous column chromatography device according to claim 1, characterized in that: The elastic metal sealing groove (12) comprises an annular base, which is fixed to the end of the column (11); and two elastic metal arms, which extend inward from the inner edge of the annular base to form a U-shaped structure.

5. The closed continuous column chromatography device according to claim 1 or 4, characterized in that: The two side surfaces of the convex ring (121) are arranged obliquely and gradually narrow from the top downwards. Form a trapezoidal outline.

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