Micro-fluidic chip

Abstract

The micro-fluidic chip comprises a disc and a sample injection cavity, a diluent cavity, a sample liquid quantifying cavity, a diluent quantifying cavity and a mixing cavity which are arranged on the disc, the mixing cavity is provided with a sample liquid inlet and a diluent inlet, the sample liquid inlet is communicated with the sample liquid quantifying cavity, and the diluent inlet is communicated with the diluent quantifying cavity; a uniform premixing structure is arranged in the uniform mixing cavity, and the uniform premixing structure is used for enabling the sample liquid and the diluent which enter the uniform mixing cavity to be intersected and mixed. As the uniform mixing cavity is internally provided with the uniform premixing structure, the uniform premixing structure can guide the diluent to flow to be intersected and uniformly mixed with the sample liquid, so that the sample liquid is fully diluted and mixed after entering the uniform mixing cavity, and meanwhile, the sample liquid can be prevented from being directly discharged from the uniform mixing cavity to the detection hole without being diluted, so that the sample liquid and the diluent can be fully mixed; therefore, the detection accuracy is ensured.

Description

Technical Field

[0001] The utility model relates to the technical field of in vitro detection, and particularly relates to a microfluidic chip. Background Art

[0002] Point-of-care testing (POCT), as a subdivision in the field of in vitro diagnosis, has been widely applied in the fields of clinical inspection, personal health management, disease prevention and monitoring, etc., due to its characteristics of miniaturized instruments, rapid detection, and simple operation.

[0003] Biochemical point-of-care testing mainly adopts centrifugal microfluidic technology. Through specific centrifugal movement of a microfluidic chip inside a biochemical analyzer, the whole process of biochemical detection operations such as sample quantification, diluent quantification, sample mixing, sample injection, and sample detection can be realized.

[0004] Among them, the samples to be detected are mainly blood samples. There are often some high-viscosity samples in blood samples, such as blood lipids, hemolysis, etc. Also, the blood viscosity of animals will increase when they are dehydrated. Under such samples, it is difficult to mix the sample and the diluent well. If the mixing is not sufficient, it will affect the test error. Content of the Utility Model

[0005] A microfluidic chip of the utility model is used to solve the problem that it is difficult to mix the sample and the diluent well.

[0006] In one embodiment, a microfluidic chip is provided, which includes a disc and a sample injection chamber, a diluent chamber, a sample liquid quantification chamber, a diluent quantification chamber, and a mixing chamber arranged on the disc;

[0007] The sample injection chamber is used for injecting sample liquid;

[0008] The diluent chamber is used for accommodating diluent;

[0009] The sample liquid quantification chamber is communicated with the sample injection chamber so that the sample liquid in the sample injection chamber flows into the sample liquid quantification chamber, and the sample liquid quantification chamber is used for quantifying the sample liquid;

[0010] The diluent quantification chamber is communicated with the diluent chamber so that the diluent in the diluent chamber flows into the diluent quantification chamber, and the diluent quantification chamber is used for quantifying the diluent;

[0011] The mixing chamber has a sample liquid inlet and a diluent inlet. The sample liquid inlet is communicated with the sample liquid quantification chamber so that the quantified sample liquid in the sample liquid quantification chamber flows into the mixing chamber; the diluent inlet is communicated with the diluent quantification chamber so that the quantified diluent in the diluent quantification chamber flows into the mixing chamber and forms a mixed liquid with the flowing-in sample liquid;

[0012] Among them, a pre-mixing structure is provided in the mixing cavity, and the pre-mixing structure is used to make the sample liquid and the diluent entering the mixing cavity intersect and mix.

[0013] In one embodiment, the pre-mixing structure includes a diversion member, and the diversion member extends from a position near the diluent inlet to a position near the sample liquid inlet, and the diversion member is used to divert the diluent to the vicinity of the sample liquid inlet.

[0014] In one embodiment, the diversion member has a diversion surface, one end of the diversion surface faces the diluent inlet, and the other end of the diversion surface faces the sample liquid inlet.

[0015] In one embodiment, the diversion surface is a concave arc surface.

[0016] In one embodiment, the diversion member is a diversion bar or a diversion sheet, and the diversion surface is located on the side surface of the diversion bar or the diversion sheet.

[0017] In one embodiment, the pre-mixing structure further includes a buffer member, and the buffer member is arranged at a position near the sample liquid inlet, and the buffer member is used to block the sample liquid flowing into the mixing cavity to reduce the flow rate of the sample liquid.

[0018] In one embodiment, the buffer member is closer to the sample liquid inlet than the diversion member, and one end of the diversion member extends to the side of the buffer member facing away from the sample liquid inlet.

[0019] In one embodiment, the buffer member has a concave portion, the opening of the concave portion faces the sample liquid inlet, and the concave portion is used to buffer and store the sample liquid flowing into the mixing cavity.

[0020] In one embodiment, the plane where the opening of the concave portion is located is inclined with respect to the flowing direction of the sample liquid at the sample liquid inlet, and the plane where the opening of the concave portion is located is inclined towards the diluent inlet direction, so that the sample liquid buffered by the concave portion overflows towards the side close to the diluent inlet.

[0021] In one embodiment, the buffer member is an arc-shaped structure, and the arc-shaped structure forms the concave portion.

[0022] In one embodiment, the disc includes a disc body and a cover plate, the sample injection cavity, the diluent cavity, the sample liquid metering cavity, the diluent metering cavity and the mixing cavity are arranged on the disc body, and the diversion member and / or the buffer member are arranged on the cover plate.

[0023] A microfluidic chip according to the above embodiments. Since a pre-mixing structure is provided in the mixing cavity, the pre-mixing structure can guide the diluent to flow to the intersection with the sample liquid for mixing, so that the sample liquid can be fully diluted and mixed after entering the mixing cavity. At the same time, it can prevent the sample liquid from being directly discharged from the mixing cavity to the detection hole without dilution, thereby ensuring that the sample liquid and the diluent are fully mixed to ensure the accuracy of the detection. Description of the Drawings

[0024] Figure 1 It is a schematic structural diagram of a microfluidic chip in an embodiment;

[0025] Figure 2 It is a schematic structural diagram of a cover plate in an embodiment;

[0026] Figure 3 It is a schematic partial structural diagram of a microfluidic chip in an embodiment;

[0027] Figure 4 It is a schematic partial structural diagram of a microfluidic chip in an embodiment;

[0028] Figure 5 It is a schematic partial structural diagram of a microfluidic chip in an embodiment;

[0029] Figure 6 It is a schematic partial structural diagram of a microfluidic chip in an embodiment;

[0030] Figure 7 It is a schematic structural diagram of a microfluidic chip in an embodiment;

[0031] Figure 8 It is a schematic structural diagram of a microfluidic chip in an embodiment;

[0032] The reference numerals are as follows:

[0033] 100 - disc, 101 - disc body, 102 - cover plate;

[0034] 1 - sample injection cavity, 2 - diluent cavity, 3 - sample liquid metering cavity, 4 - diluent metering cavity, 5 - mixing cavity, 51 - sample liquid inlet, 52 - diluent inlet, 53 - mixed liquid outlet, 6 - pre-mixing structure, 61 - deflector, 611 - deflector surface, 62 - buffer, 621 - concave portion, 7 - first capillary, 8 - second capillary, 9 - detection hole, 10 - diluent accommodation cavity. Detailed Embodiments

[0035] The following further elaborates on the present utility model in detail through specific embodiments in conjunction with the accompanying drawings. Similar elements in different embodiments are labeled with related similar element numbers. In the following embodiments, many detailed descriptions are provided to enable a better understanding of the present application. However, those skilled in the art can easily recognize that some of the features can be omitted in different situations, or can be replaced by other elements, materials, or methods. In some cases, some operations related to the present application are not shown or described in the specification to avoid overwhelming the core part of the present application with excessive descriptions. For those skilled in the art, it is not necessary to describe these related operations in detail, and they can fully understand the related operations based on the descriptions in the specification and general technical knowledge in the art.

[0036] In addition, the features, operations, or characteristics described in the specification can be combined in any appropriate manner to form various embodiments. At the same time, the steps or actions in the method description can also be reordered or adjusted in an obvious manner by those skilled in the art. Therefore, the various sequences in the specification and drawings are only for clearly describing a certain embodiment and do not mean that they are the necessary sequences, unless it is stated that a certain sequence must be followed.

[0037] The serial numbers assigned to the components herein, such as "first", "second", etc., are only used to distinguish the described objects and do not have any sequential or technical meaning. And the "connection" and "coupling" mentioned in this application, unless otherwise specified, both include direct and indirect connection (coupling). The up and down directions herein are the directions in the centrifugal detection state of the microfluidic chip.

[0038] In one embodiment, a microfluidic chip is provided. This microfluidic chip is a disc for biochemical point-of-care testing. This microfluidic chip mainly uses centrifugal microfluidic technology to achieve detection. Through the specific centrifugal movement of the microfluidic chip inside the biochemical analyzer, the full process operations of biochemical detection such as sample quantification, diluent quantification, sample mixing, sample injection, and sample detection are realized.

[0039] In this embodiment, a pre-mixing structure is provided in the mixing cavity. The pre-mixing structure can guide the diluent entering the mixing cavity to the sample liquid inlet of the mixing cavity, so that the sample can be mixed with the diluent first after entering the mixing cavity, which can avoid the sample liquid entering the mixing cavity from being directly discharged without contacting the diluent, so as to ensure the dilution and mixing of the sample liquid.

[0040] Furthermore, the pre-mixing structure can also be used to block the sample liquid entering the mixing cavity to reduce the flow rate of the sample liquid in the mixing cavity, achieving a buffering effect, making the flow rate of the sample liquid in the mixing cavity lower than that of the diluent, so that the sample liquid will intersect and mix with more diluent per unit time, thereby ensuring the dilution and mixing of the sample liquid.

[0041] Furthermore, the pre-mixing structure can also have a certain storage function for storing the sample liquid that first enters the mixing chamber. The sample liquid that initially enters the mixing chamber first enters the pre-mixing structure. When the storage part is filled, it overflows, which can prevent the sample liquid from entering the mixing chamber before the diluent and being directly discharged without being diluted and mixed. That is, the sample liquid will consume a certain amount of time during the buffering process, which can make up for the time difference of the late entry of the diluent into the mixing chamber, so that there is diluent in the mixing chamber before the sample liquid is discharged from the mixing chamber, thereby ensuring that the sample liquid can be mixed with the diluent in the mixing chamber.

[0042] Please refer to Figures 1 to 3 , the microfluidic chip of this embodiment mainly includes a disc 100. The disc 100 is of a disc-shaped structure. The disc-shaped disc 100 is conducive to realizing the full process operations of biochemical detection such as sample quantification, diluent quantification, sample mixing, sample injection, and sample detection during the centrifugal motion process.

[0043] The disc 100 is provided with a sample injection chamber 1, a diluent chamber 2, a sample liquid quantification chamber 3, a diluent quantification chamber 4, and a mixing chamber 5. The disc 100 is also provided with necessary structures such as a capillary and a detection hole 9 for realizing detection.

[0044] The sample injection chamber 1 is used for injecting the sample liquid. The sample injection chamber 1 can be provided with an injection port, and the sample liquid can be injected into the sample injection chamber 1 through the injection port.

[0045] The diluent chamber 2 is used to accommodate the diluent. A diluent accommodating chamber 10 can be provided in the middle of the disc 100. The diluent accommodating chamber 10 is used to install a diluent package. The diluent package contains the diluent. The diluent accommodating chamber 10 has an opening communicating with the diluent chamber 2. After the opening of the diluent package is opened, under the action of centrifugal force, the diluent in the diluent package can flow into the diluent chamber 2.

[0046] The sample liquid quantification chamber 3 can be connected to the sample injection chamber 1 through a capillary so that the sample liquid in the sample injection chamber 1 flows into the sample liquid quantification chamber 3. The sample liquid quantification chamber 3 is used for quantifying the sample liquid. The sample liquid quantification chamber 3 can be connected to a cavity such as a sample liquid waste chamber for collecting the sample liquid. When too much sample liquid enters the sample liquid quantification chamber 3, it will overflow into the sample liquid waste chamber to ensure that the quantified sample liquid in the sample liquid quantification chamber 3 is the amount required for testing and guarantee the accuracy of the sample dilution ratio.

[0047] The dilution liquid metering chamber 4 can be connected to the dilution liquid chamber 2 through a capillary tube so that the dilution liquid in the dilution liquid chamber 2 flows into the dilution liquid metering chamber 4. The dilution liquid metering chamber 4 is used for metering the dilution liquid. The dilution liquid metering chamber 4 can be connected to a chamber such as a dilution liquid waste chamber for collecting the dilution liquid. After too much dilution liquid enters the dilution liquid metering chamber 4, it will overflow into the dilution liquid waste chamber to ensure that the metered dilution liquid in the dilution liquid metering chamber 4 is the amount required for testing and guarantee the accurate proportion of sample dilution.

[0048] The mixing chamber 5 has a sample liquid inlet 51 and a dilution liquid inlet 52. The sample liquid inlet 51 of the mixing chamber 5 can be connected to the sample liquid metering chamber 3 through a first capillary tube 7. One end of the first capillary tube 7 is connected to the sample liquid metering chamber 3, and the other end of the first capillary tube 7 is connected to the sample liquid inlet 51 of the mixing chamber 5. The first capillary tube 7 is used to divert the sample liquid in the sample liquid metering chamber 3 into the mixing chamber 5.

[0049] The dilution liquid inlet 52 of the mixing chamber 5 can be connected to the dilution liquid metering chamber 4 through a second capillary tube 8. One end of the second capillary tube 8 is connected to the dilution liquid metering chamber 4, and the other end of the second capillary tube 8 is connected to the dilution liquid inlet 52 of the mixing chamber 5. The second capillary tube 8 is used to divert the dilution liquid in the dilution liquid metering chamber 4 into the mixing chamber 5.

[0050] The mixing chamber 5 provides an environmental place for diluting and mixing the sample liquid and the dilution liquid. The sample liquid and the dilution liquid enter the mixing chamber 5 and intersect and mix to form a mixed liquid. The mixing chamber 5 also has a mixed liquid outlet 53. The mixed liquid outlet 53 of the mixing chamber 5 is connected to a plurality of detection holes 9 through a capillary tube and a liquid distribution chamber in sequence. The liquid distribution chamber can be provided with one inlet and a plurality of outlets. The inlet of the liquid distribution chamber is connected to the mixed liquid outlet 53 of the mixing chamber 5 through a capillary tube, and the plurality of outlets of the liquid distribution chamber are correspondingly connected to the plurality of detection holes 9 one by one. The liquid distribution chamber can distribute the mixed liquid led out from the mixing chamber 5 into the plurality of detection holes 9, and the plurality of detection holes 9 can realize simultaneous detection of multiple items.

[0051] In this embodiment, a pre-mixing structure 6 is provided in the mixing chamber 5, and the pre-mixing structure 6 is used to make the sample liquid and the dilution liquid entering the mixing chamber 5 intersect and mix.

[0052] The pre-mixing structure 6 can include a diversion member 61. The diversion member 61 extends from a position near the dilution liquid inlet 52 to a position near the sample liquid inlet 51. The diversion member 61 is used to divert the dilution liquid to the vicinity of the sample liquid inlet 51, that is, the dilution liquid entering the mixing chamber 5 from the dilution liquid inlet 52 will be diverted by the diversion member 61 to the vicinity of the sample liquid inlet 51, so that the sample liquid entering the mixing chamber 5 from the sample liquid inlet 51 intersects and mixes with the dilution liquid, and it can be avoided that the sample liquid directly discharges from the mixed liquid outlet 53 without being diluted and mixed after entering the mixing chamber 5.

[0053] The flow guide member 61 can be a flow guide bar or a flow guide sheet or other flow guide structures. The flow guide bar or the flow guide sheet has a flow guide surface 611 perpendicular to the rotation plane of the disk 100. Of course, if the flow guide surface 611 is inclined at a certain angle relative to the rotation plane of the disk 100, it can also play a role in guiding the flow. The sample liquid inlet 51 and the diluent inlet 52 are located on one side of the mixing chamber 5 close to the rotation axis of the disk 100, and the flow guide surface 611 faces the direction of the rotation axis of the disk 100. Among them, it is ensured that the flow guide surface 611 faces the sample liquid inlet 51 and the diluent inlet 52. One end of the flow guide surface 611 is close to and faces the diluent inlet 52, and the other end of the flow guide surface 611 is close to and faces the sample liquid inlet 51. The diluent flowing into the mixing chamber 5 from the diluent inlet 52 will flow to the flow guide surface 611, and under the guidance of the flow guide surface 611, the diluent is guided to a position close to the sample liquid inlet 51, so that the diluent entering the mixing chamber 5 can reach the position of the sample liquid inlet 51 as soon as possible, avoiding the sample liquid flowing in from the sample liquid inlet 51 being directly discharged from the mixing liquid outlet 53 without being diluted and mixed. The setting of the flow guide member 61 can ensure that the sample liquid is fully diluted and mixed, thereby ensuring the accuracy of sample detection.

[0054] Among them, the flow guide member 61 is a flow guide bar or a flow guide sheet, which can reduce the structure of the flow guide member 61 and reduce the occupied space of the flow guide member 61.

[0055] In other embodiments, the flow guide member 61 can also be other structures. For example, the flow guide member 61 is a flow guide block, a flow guide plate or other structures, and the side surfaces of the flow guide block and the flow guide plate can also form a flow guide surface 611 to achieve the effect of guiding the diluent.

[0056] In this embodiment, the flow guide surface 611 can be a concave arc surface, and the concave direction of the concave arc surface is away from the center direction of the disk 100. The concave arc surface can play a better role in guiding the flow. When the flow guide surface 611 blocks and guides the diluent, the arc-shaped flow guide surface 611 can make full use of the kinetic energy of the diluent, reduce the impact and speed reduction generated when blocking and guiding the diluent, and ensure that the diluent has a relatively fast flow rate to reach near the sample liquid inlet 51 to improve the dilution effect of the sample liquid.

[0057] Please refer to Figure 4 , in other embodiments, the flow guide surface 611 can also be other structures. For example, the flow guide surface 611 is a plane, and it can also achieve guiding the diluent from the position of the diluent inlet 52 to the position of the sample liquid inlet 51.

[0058] Please refer to Figure 5 , in other embodiments, the flow guide surface 611 is a combined structure of a plane and a curved surface or a combined structure of different inclined planes, etc., and it can also achieve guiding the diluent from the position of the diluent inlet 52 to the position of the sample liquid inlet 51.

[0059] In this embodiment, the pre-mixing structure 6 may further include a buffer 62, which is located in the mixing chamber 5. The buffer 62 is arranged near the sample liquid inlet 51, and the buffer 62 is closer to the sample liquid inlet 51 than the guide 61, so that the sample liquid entering the mixing chamber 5 from the sample liquid inlet 51 will first contact the buffer 62, and the buffer 62 is used to block the sample liquid flowing into the mixing chamber 5. The sample liquid will lose kinetic energy after being blocked by the buffer 62, so as to reduce the flow rate of the sample liquid, so that the flow rate of the sample liquid is lower than the flow rate of the diluent, so that the diluent can reach the mixing liquid outlet 53 earlier than the sample liquid, which can prevent the sample liquid from flowing directly from the mixing liquid outlet 53 to the detection hole 9 without being diluted with the diluent.

[0060] One end of the flow guide 61 may extend to the side of the buffer 62 facing away from the sample liquid inlet 51, or one end of the flow guide 61 may extend to a position between the buffer 62 and the mixing liquid outlet 53. In this way, the sample liquid is first mixed with the diluent when entering the mixing liquid outlet 53, so that the dilution ratio of the sample liquid can be guaranteed.

[0061] In this embodiment, the buffer member 62 has an inner concave portion 621, the opening of the inner concave portion 621 faces the sample liquid inlet 51, and the inner concave portion 621 forms a buffer cavity with an opening. When the sample liquid enters the mixing chamber 5 from the sample liquid inlet 51, the sample liquid will first enter the inner concave portion 621 of the buffer member 62 and gather in the inner concave portion 621. As the sample liquid continues to flow in, the inner concave portion 621 is filled with the sample liquid, and the subsequent inflow of sample liquid will overflow from the opening of the inner concave portion 621, and the overflowed sample liquid will be mixed with the diluent.

[0062] The inner concave portion 621 of the buffer member 62 can buffer and slow down the sample liquid, and can also buffer the sample liquid that first enters the mixing chamber 5, so that when the sample liquid first enters the mixing chamber 5, it will not directly flow out from the mixing liquid outlet 53, that is, the inner concave portion 621 can delay the sample liquid from flowing out of the mixing chamber 5, so as to offset the time difference of the diluent flowing to the position close to the sample liquid inlet 51. When the sample liquid overflows from the inner concave portion 621, the diluent has been guided by the guide member 61 to the vicinity of the buffer member 62, which can ensure that the sample and the diluent are fully mixed.

[0063] In this embodiment, the buffer 62 may be an arc-shaped structure, and the inner concave portion 621 is formed by bending the arc-shaped structure. In other embodiments, the buffer 62 may also be other structures, and the buffer 62 may be a block structure with a groove, for example, the buffer 62 is a U-shaped structure, and the inner concave portion 621 may also be formed to achieve buffering and storage of the sample liquid.

[0064] Please refer to Figure 6, in other embodiments, the buffer member 62 may be of a linear structure and does not include the concave portion 621. The linear buffer member 62 can block and decelerate the inflowing sample liquid, and can also delay the direct entry of the sample liquid into the mixing liquid outlet 53. Furthermore, it can increase the mixing time of the sample liquid and the diluent, and can also ensure the sufficient mixing of the sample liquid and the diluent. Among them, the buffer member 62 can also be inclined relative to the flow guide member 61 to play a certain guiding role, guiding the sample liquid to the flow guide member 61, which can improve the mixing effect of the sample liquid and the diluent.

[0065] In this embodiment, the sample liquid inlet 51 and the diluent inlet 52 are located at the upper end positions on the side of the mixing cavity 5. Preferably, the flow guide member 61 and the buffer member 62 can be provided only at the upper end of the mixing cavity 5, and in the direction of the rotation axis of the parallel disk 100, the heights of the flow guide member 61 and the buffer member 62 are equal to or slightly greater than the depths of the sample liquid inlet 51 and the diluent inlet 52. While ensuring that the flow guide member 61 and the buffer member 62 can respectively achieve flow guiding and buffering, the structures of the flow guide member 61 and the buffer member 62 are reduced and simplified to reduce the occupied space of the flow guide member 61 and the buffer member 62.

[0066] In other embodiments, the flow guide member 61 and the buffer member 62 can also extend from the upper end of the mixing cavity 5 to the middle or bottom position of the mixing cavity 5.

[0067] In this embodiment, the disk 100 may include a disk body 101 and a cover plate 102. The disk body 101 is the main structure of the disk 100, and a groove body or the like is provided on one side of the disk body 101. The cover plate 102 can be fixed to the disk body 101 by means of bonding, heat sealing, etc.

[0068] The sample injection cavity 1, the diluent cavity 2, the sample liquid metering cavity 3, the diluent metering cavity 4, the mixing cavity 5, the detection hole 9, and the capillary are all provided on the disk body 101, and the flow guide member 61 and the buffer member 62 are provided on the cover plate 102. By providing the flow guide member 61 and the buffer member 62 on the cover plate 102, it is easy to arrange the flow guide member 61 and the buffer member 62 at the upper end of the mixing cavity 5, that is, it is easy to make the flow guide member 61 and the buffer member 62 be in the same plane as the sample liquid inlet 51 and the diluent inlet 52; at the same time, the structures of the flow guide member 61 and the buffer member 62 can be simplified.

[0069] The flow guide member 61 and the buffer member 62 can be integrally formed with the cover plate 102, or the flow guide member 61 and the buffer member 62 can also be fixed to the cover plate 102 by means of bonding, etc.

[0070] In other embodiments, one of the flow guiding member 61 and the buffer member 62 is disposed on the cover plate 102, and the other is disposed on the disk body 101 through a mounting frame or directly; alternatively, both the flow guiding member 61 and the buffer member 62 are disposed on the disk body 101 through a mounting frame or directly. With such an arrangement, it is also possible to dispose the flow guiding member 61 and the buffer member 62 in the same plane as the sample liquid inlet 51 and the diluent inlet 52, so as to achieve the guiding of the diluent and the buffering and storage of the sample liquid.

[0071] In this embodiment, since the flow guiding member 61 and the buffer member 62 are provided in the mixing chamber 5, the flow guiding member 61 can guide the diluent entering the mixing chamber 5 to the vicinity of the sample liquid inlet 51, and the buffer member 62 can buffer and store the sample liquid entering the mixing chamber 5, so as to achieve guiding the diluent to intersect and mix with the sample liquid, so that the sample liquid can be fully diluted and mixed after entering the mixing chamber 5, and at the same time, it can be avoided that the sample liquid is directly discharged from the mixing chamber 5 to the detection hole 9 without dilution, thereby ensuring the dilution concentration of the sample liquid and ensuring the accuracy of the detection.

[0072] In one embodiment, the plane where the opening of the concave portion 621 is located is inclined with respect to the flowing direction of the sample liquid of the flow guiding member 61, and the plane where the opening of the concave portion 621 is located is inclined towards the flow guiding member 61, that is, the opening of the concave portion 621 can be inclined relative to the flow guiding member 61, so that the sample liquid buffered by the concave portion 621 overflows towards the side closer to the flow guiding member 61. With such an arrangement, the sample liquid can overflow to the side with more diluent, improving the dilution and mixing effect of the sample liquid.

[0073] In one embodiment, the flow resistance of the first capillary 7 is greater than that of the second capillary 8, and the flowing speed of the diluent into the mixing chamber 5 is greater than the flowing speed of the sample liquid into the mixing chamber 5. The faster flowing diluent can reach the mixing liquid outlet 53 earlier than the sample liquid, and it can be avoided that the sample liquid reaches the mixing liquid outlet 53 first and directly flows into the detection hole 9, ensuring that the sample liquid will intersect and mix with the diluent after entering the mixing chamber 5 and before flowing into the mixing liquid outlet 53.

[0074] The diluent with a fast flowing speed can also form an impact disturbance on the sample liquid, achieving a better dilution and mixing effect.

[0075] Among them, the cross-sectional area of the first capillary 7 is greater than that of the second capillary 8, that is, the diameter of the first capillary 7 is greater than that of the second capillary 8, thereby making the flow resistance of the first capillary 7 greater than that of the second capillary 8.

[0076] In other embodiments, the first capillary 7 is more straight than the second capillary 8, that is, the second capillary 8 has more turning structures or a smaller turning radius, and it is also possible to achieve that the flow resistance of the first capillary 7 is greater than that of the second capillary 8.

[0077] Please refer toFigure 7 In one embodiment, a microfluidic chip is provided. The difference between the microfluidic chip in this embodiment and the microfluidic chips in any of the above embodiments is that the pre-mixing structure 6 only includes a flow guiding member 61 and does not include a buffer member 62.

[0078] The flow guiding member 61 is used to guide the diluent to the vicinity of the sample liquid inlet 51. That is, the diluent entering the mixing chamber 5 from the diluent inlet 52 will be guided by the flow guiding member 61 to the vicinity of the sample liquid inlet 51, so that the sample liquid entering the mixing chamber 5 from the sample liquid inlet 51 can immediately intersect and mix with the diluent, and it can be avoided that the sample liquid directly discharges from the mixing liquid outlet 53 without being diluted and mixed after entering the mixing chamber 5.

[0079] The microfluidic chip in this embodiment, compared with the prior art, includes the pre-mixing structure 6 with the flow guiding member 61, and also improves the dilution and mixing effect of the sample liquid, thereby improving the accuracy of detection.

[0080] Please refer to Figure 8 In one embodiment, a microfluidic chip is provided. The difference between the microfluidic chip in this embodiment and the microfluidic chips in any of the above embodiments is that the pre-mixing structure 6 only includes a buffer member 62 and does not include a flow guiding member 61.

[0081] The buffer member 62 is located in the mixing chamber 5. The buffer member 62 is arranged at a position close to the sample liquid inlet 51, and the buffer member 62 is closer to the sample liquid inlet 51 than the flow guiding member 61. So that the sample liquid entering the mixing chamber 5 from the sample liquid inlet 51 will first contact the buffer member 62. The buffer member 62 is used to block the sample liquid flowing into the mixing chamber 5. After being blocked by the buffer member 62, the sample liquid will lose kinetic energy to reduce the flow rate of the sample liquid, so that the flow rate of the sample liquid is lower than that of the diluent. In this way, the diluent can reach the mixing liquid outlet 53 earlier than the sample liquid, and it can be avoided that the sample liquid directly flows into the detection hole 9 from the mixing liquid outlet 53 without being diluted with the diluent.

[0082] The microfluidic chip in this embodiment, compared with the prior art, includes the pre-mixing structure 6 with the buffer member 62, and also improves the dilution and mixing effect of the sample liquid, thereby improving the accuracy of detection.

[0083] The above uses specific examples to elaborate on the present invention, which is only used to help understand the present invention and is not intended to limit the present invention. For those skilled in the technical field to which the present invention belongs, according to the idea of the present invention, several simple deductions, deformations or replacements can also be made.

Claims

1. A microfluidic chip, characterized in that: It comprises a disk (100) and a sample injection chamber (1), a diluent chamber (2), a sample liquid quantitative chamber (3), a diluent quantitative chamber (4) and a mixing chamber (5) arranged on the disk (100); The sample injection chamber (1) is used for injecting sample liquid; The diluent chamber (2) is used to contain the diluent; The sample liquid quantitative chamber (3) is in communication with the sample injection chamber (1) so that the sample liquid in the sample injection chamber (1) flows into the sample liquid quantitative chamber (3), and the sample liquid quantitative chamber (3) is used to quantify the sample liquid; The diluent quantitative chamber (4) is in communication with the diluent chamber (2) so that the diluent in the diluent chamber (2) flows into the diluent quantitative chamber (4), and the diluent quantitative chamber (4) is used to quantitatively measure the diluent; The mixing chamber (5) comprises a sample liquid inlet (51) and a diluent inlet (52); the sample liquid inlet (51) is connected to the sample liquid quantitative chamber (3) so that the quantitative sample liquid in the sample liquid quantitative chamber (3) flows into the mixing chamber (5); the diluent inlet (52) is connected to the diluent quantitative chamber (4) so ​​that the quantitative diluent in the diluent quantitative chamber (4) flows into the mixing chamber (5) and forms a mixed liquid with the inflowing sample liquid; Wherein, a pre-mixing structure (6) is provided in the mixing chamber (5), and the pre-mixing structure (6) is used to allow the sample liquid and the diluent entering the mixing chamber (5) to intersect and mix.

2. The microfluidic chip according to claim 1, characterized in that: The pre-mixing structure (6) comprises a flow guide (61), wherein the flow guide (61) extends from a position close to the diluent inlet (52) to a position close to the sample liquid inlet (51), and the flow guide (61) is used to guide the diluent to the vicinity of the sample liquid inlet (51).

3. The microfluidic chip according to claim 2, characterized in that: The flow guide member (61) has a flow guide surface (611), one end of the flow guide surface (611) faces the diluent inlet (52), and the other end of the flow guide surface (611) faces the sample liquid inlet (51).

4. The microfluidic chip according to claim 3, characterized in that: The flow-guiding surface (611) is an inwardly concave arc surface.

5. The microfluidic chip according to claim 3, characterized in that: The guide member (61) is a guide strip or a guide plate, and the guide surface (611) is located on the side of the guide strip or the guide plate.

6. The microfluidic chip according to any one of claims 2 to 5, characterized in that: The pre-mixing structure (6) further comprises a buffer (62), wherein the buffer (62) is arranged at a position close to the sample liquid inlet (51), and the buffer (62) is used to block the sample liquid flowing into the mixing chamber (5) to reduce the flow rate of the sample liquid.

7. The microfluidic chip according to claim 6, characterized in that: The buffer member (62) is closer to the sample liquid inlet (51) than the flow guide member (61), and one end of the flow guide member (61) extends to a side of the buffer member (62) facing away from the sample liquid inlet (51).

8. The microfluidic chip according to claim 6, characterized in that: The buffer member (62) has an inner recess (621), the opening of the inner recess (621) faces the sample liquid inlet (51), and the inner recess (621) is used to buffer and store the sample liquid flowing into the mixing chamber (5).

9. The microfluidic chip according to claim 8, characterized in that: The surface where the opening of the inner concave portion (621) is located is inclined relative to the sample liquid inflow direction of the sample liquid inlet (51), and the surface where the opening of the inner concave portion (621) is located is inclined toward the diluent inlet (52), so that the sample liquid buffered by the inner concave portion (621) overflows to a side close to the diluent inlet (52).

10. The microfluidic chip according to claim 8, characterized in that: The buffer member (62) is an arc-shaped structure, and the arc-shaped structure forms the inner concave portion (621).

11. The microfluidic chip according to claim 6, characterized in that: The disk (100) comprises a disk body (101) and a cover plate (102); the sample injection chamber (1), the diluent chamber (2), the sample liquid quantitative chamber (3), the diluent quantitative chamber (4) and the mixing chamber (5) are arranged on the disk body (101); and the flow guide (61) and / or the buffer (62) are arranged on the cover plate (102).

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