Environmental DNA liquid blowing device

Abstract

The utility model relates to the technical field of environmental monitoring, in particular to an environmental DNA (deoxyribonucleic acid) liquid blowing device, which comprises a box body, a plurality of groups of integrated filters, a plurality of groups of liquid blowing devices and a plurality of liquid blowing devices, the test tube supporting mechanism is used for supporting a test tube and enabling the test tube to be positioned below the integrated filter; the gas power mechanism is used for providing positive pressure gas for the inlet of the integrated filter, so that the liquid in the liquid storage box enters the test tube. The test tube supporting mechanism and the gas power mechanism are arranged, the test tube supporting mechanism can move test tubes to the position below the integrated filters, the gas power mechanism can provide positive pressure gas for the multiple sets of placed integrated filters, and the positive pressure gas can enable liquid in the liquid storage box to penetrate through filter membranes of the filters; therefore, the automatic liquid blowing of the multiple groups of integrated filters is realized.

Description

Technical Field

[0001] The utility model relates to the technical field of environmental monitoring, and particularly relates to an environmental DNA liquid blowing device. Background Art

[0002] Environmental DNA, or eDNA, refers to a method of directly sampling DNA fragments from environmental samples (such as soil, sediment, air, and water bodies) and then using sequencing technology for qualitative or quantitative analysis. In recent years, with the development of molecular biology, the eDNA technology has become a new method for aquatic biological investigation, mainly used for the detection and prevention of biological invasions, the protection of endangered species, the evaluation of biodiversity, the assessment of biomass, the real-time species distribution and community diversity information, tracking the life history process of species, and reflecting the changes in ecosystem structure. The collection and detection of eDNA have the advantages of being environmentally friendly, easy to sample, and highly sensitive. It has good applicability and importance in the fields of ecological environment detection and environmental science. Its development provides a new means for biodiversity detection and has become a research hotspot in ecology and environmental science.

[0003] For example, the Chinese utility model patent with the application number CN202223580623.X discloses a nitrogen blowing instrument that is convenient to control the feeding. The nitrogen blowing instrument includes a base and a sample tray arranged at its upper end. The sample tray is provided with test tubes for placing samples. Above the base, there is a positioning plate, and the positioning plate is provided with a blowing structure for blowing air inside the test tubes. The right side of the positioning plate is connected with a lifting push member for driving it to move up and down. The lifting push member includes a lifting vertical plate arranged at the upper end of the base, and a lifting slider is slidably arranged on the lifting vertical plate.

[0004] Another example is the Chinese utility model patent with the application number CN202321435027.4, which discloses an eDNA collection and analysis system, including several sampling points distributed at different positions. The sampling points include indoor sampling points, outdoor sampling points, and water sampling points. The indoor sampling points, outdoor sampling points, and water sampling points are single points, multiple points, or linearly distributed along the basin. The medium water in the natural environment collected by the indoor sampling points, outdoor sampling points, and water sampling points includes river water, lake water, river water, seawater, or groundwater. The indoor sampling points are provided with sampling rooms, and indoor collectors are arranged in the sampling rooms. Outdoor collectors are arranged at the outdoor sampling points, and buoy collectors or floating drum collectors are arranged at the water sampling points. Integrated filters for filtering and collecting eDNA are respectively arranged on the indoor collectors, outdoor collectors, buoy collectors, and floating drum collectors.

[0005] After collecting the environmental DNA samples using the integrated filters, it is necessary to transfer the liquid samples in the integrated filters to test tubes, but there is a lack of equipment in the prior art that can transfer the liquid samples in multiple groups of integrated filters. Summary of the Utility Model

[0006] To solve the above problems, the purpose of the present utility model is to provide an environmental DNA liquid blowing device.

[0007] The technical solution provided by the present utility model is as follows:

[0008] An environmental DNA liquid blowing device, comprising a box body, which is provided with:

[0009] Multiple groups of integrated filters, and each integrated filter is provided with a liquid storage box for storing liquid;

[0010] A test tube supporting mechanism, which is used to support the test tube and make the test tube located below the integrated filter;

[0011] A gas power mechanism, which is used to provide positive pressure gas to the inlet of the integrated filter, so that the liquid in the liquid storage box enters the test tube.

[0012] As an optional technical solution, the gas power mechanism includes N 1 air pumps, each air pump is connected with N 2 solenoid valves, and each solenoid valve is connected with a gas nozzle; the gas nozzle is used to dock with the inlet of the integrated filter;

[0013] N 1 is a positive integer, and N 1 ≥1;

[0014] N 2 is a positive integer, and N 2 ≥1.

[0015] Optionally, the gas nozzle is installed on a pressing plate, and the pressing plate is connected with a first driving mechanism; the first driving mechanism is used to drive the pressing plate to perform vertical linear motion.

[0016] Optionally, an elastic sealing suction cup is installed at the docking end of the gas nozzle and the inlet of the integrated filter.

[0017] Furthermore, the integrated filter is installed on a bracket, and the bracket is connected with a second driving mechanism, and the second driving mechanism is used to drive the bracket to perform horizontal linear motion; the test tube supporting mechanism includes a tray and a third driving mechanism connected with the tray, and the third driving mechanism is used to drive the tray to perform horizontal linear motion.

[0018] Optionally, the tray is detachably connected with a test tube rack, and the test tube rack is used to store test tubes.

[0019] Optionally, the second driving mechanism and the third driving mechanism are respectively located on the side of the box body; the first driving mechanism is located on the side of the box body.

[0020] Optionally, the second driving mechanism is a pressing type elastic self-locking mechanism, which is located on both sides of the box body; the third driving mechanism is located at the bottom of the box body; the first driving mechanism is located on the back of the box body.

[0021] Further, the bracket is connected to the connecting frame through a buffer mechanism, and the connecting frame is connected to the second driving mechanism.

[0022] Further, a limiting mechanism is provided between the bracket and the pressing plate; the limiting mechanism includes a plug rod fixedly connected to the pressing plate, a threaded portion is provided at the lower end of the plug rod, and a nut is installed on the threaded portion; a jack adapted to the plug rod is provided on the connecting frame.

[0023] As an alternative technical solution, a filter is installed at the liquid outlet of the liquid storage box. When positive pressure gas is introduced into the inlet, the liquid in the liquid storage box passes through the filter membrane of the filter and flows out from the outlet of the filter.

[0024] Adopting the technical solution provided by the present utility model, compared with the prior art, it has the following beneficial effects:

[0025] The present utility model is provided with a test tube supporting mechanism and a gas power mechanism. The test tube supporting mechanism can move the test tube to the lower part of the integrated filter, and the gas power mechanism can provide positive pressure gas to multiple groups of placed integrated filters. The positive pressure gas can make the liquid in the liquid storage box pass through the filter membrane of the filter, and then enter the lower test tube, so as to realize automatic liquid blowing for multiple groups of integrated filters.

[0026] In addition, the present utility model can use one air pump to connect multiple solenoid valves, so as to supply gas to the air nozzles connected to the solenoid valves. By setting multiple air pumps, it can be realized that one liquid blowing device can be configured with multiple air nozzles, so as to be able to blow liquid for multiple integrated filters. The present utility model can flexibly control the opening and closing of the solenoid valves according to the number of placed integrated filters and test tubes, so as to only provide positive pressure gas to the air nozzles corresponding to the integrated filters, and avoid supplying gas to the air nozzles where no integrated filters are placed. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 Schematic diagram of the structure of the liquid blowing device in an embodiment of the present application;

[0028] Figure 2 Schematic diagram when the third driving mechanism is arranged at the bottom of the box body in an embodiment of the present application;

[0029] Figure 3 Schematic diagram of the installation position of the pressing plate and the bracket in an embodiment of the present application;

[0030] Figure 4 Schematic diagram of the installation of the buffer mechanism in an embodiment of the present application;

[0031] Figure 5 Schematic diagram of the integrated filter structure in an embodiment of the present application;

[0032] Figure 6 Schematic diagram of the air nozzle structure in an embodiment of the present application;

[0033] Figure 7 Schematic diagram of the tray structure in an embodiment of the present application;

[0034] Figure 8 Schematic diagram when the second driving mechanism and the third driving mechanism are respectively located on the side of the box body in an embodiment of the present application.

[0035] Explanation of the reference numerals in the schematic diagram:

[0036] Box body 101, universal wheel 102, display screen 103, air pump 104, solenoid valve 105, test tube 201, test tube rack 202, tray 203, third driving mechanism 204, bolt 205, connecting frame 301, bracket 302, liquid storage box 303, buffer mechanism 304, inlet 305, liquid outlet 306, filter 307, second driving mechanism 308, first driving mechanism 401, pressing plate 402, air nozzle 403, insertion rod 404, threaded part 405, nut 406, jack 407, elastic sealing suction cup 408. Detailed implementation manners

[0037] To further understand the content of the present invention, the present invention will be described in detail in combination with the drawings and embodiments.

[0038] The structures, ratios, sizes, etc. shown in the drawings of this specification are only used to cooperate with the content disclosed in the specification for those skilled in this technology to understand and read, and are not used to limit the limiting conditions under which the present invention can be implemented. Therefore, they do not have technical essential meanings. Any modification of the structure, change of the proportional relationship or adjustment of the size, without affecting the effects that the present invention can produce and the purposes that can be achieved, should still fall within the scope that can be covered by the technical content disclosed in the present invention. At the same time, the terms such as "upper", "lower", "left", "right", "middle", etc. cited in this specification are only for the convenience of clear narration and are not used to limit the implementable scope. The change or adjustment of their relative relationships, without substantial change of the technical content, should also be regarded as the implementable scope of the present invention.

[0039] In one embodiment, as Figure 2 、 8As shown in the figure, the present utility model proposes an environmental DNA liquid blowing device, which includes a box body 101. Inside this box body 101, there are multiple groups of integrated filters, a test tube supporting mechanism, and a gas power mechanism. Among them, the integrated filter is provided with a liquid storage box 303 for storing liquid. After the integrated filter collects the environmental DNA sample, the environmental DNA sample is stored in the liquid storage box 303. Corresponding numbers of test tubes 201 are configured according to the number of integrated filters. The test tubes 201 are supported by the test tube supporting mechanism, and the test tubes 201 are made to be below the integrated filters through the test tube supporting mechanism, so that the positions of the test tubes 201 correspond one by one to the integrated filters.

[0040] When the test tube 201 is below the integrated filter and the positions correspond one by one, positive pressure gas is provided to the inlet 305 of the integrated filter through the gas power mechanism. The positive pressure gas compresses the liquid in the liquid storage box 303, so that the liquid in the liquid storage box 303 enters the test tube 201.

[0041] In one embodiment, the gas power mechanism includes N 1 air pumps 104. Each air pump 104 is connected to N 2 solenoid valves 105. Each solenoid valve 105 is connected to a gas nozzle 403. The solenoid valve 105 can be connected to a PLC controller, and the opening and closing of the solenoid valve 105 can be controlled through the PLC controller. This is relatively mature in the prior art and will not be elaborated here.

[0042] When positive pressure gas needs to be provided to the integrated filter, the gas nozzle 403 is docked with the inlet 305 of the integrated filter, and the corresponding air pump 104 and solenoid valve 105 are opened, so that the positive pressure gas enters the integrated filter through the gas nozzle 403.

[0043] In this embodiment, it should be noted that N 1 is a positive integer, and N 1 ≥1, N 2 is a positive integer, and N 2 ≥1. For example, if N 1 is 4 and N 2 is 8, then this device has a total of 32 gas nozzles, and at most 32 integrated filters can be blown with liquid simultaneously each time. It should be noted that N 1 and N 2 can also be other positive integers.

[0044] The present utility model can flexibly control the opening and closing of the solenoid valve 105 according to the number of integrated filters and test tubes 201 placed, that is, only the solenoid valve 105 at the corresponding position where the integrated filter is placed is opened, so that positive pressure gas is only provided to the gas nozzle 403 corresponding to the integrated filter. At this time, supplying gas to the gas nozzle 403 without an integrated filter placed can be avoided.

[0045] In one embodiment, as Figure 3 shown, the air nozzle 403 is installed on the pressing plate 402, and the pressing plate 402 is connected to the first driving mechanism 401. The first driving mechanism 401 is used to drive the pressing plate 402 to perform a vertical linear motion. In this embodiment, the air nozzle 403 is arranged above the integrated filter. When positive pressure gas needs to be provided to the integrated filter, the first driving mechanism 401 drives the pressing plate 402 to move downward towards the integrated filter, so that the air nozzle 403 is docked with the integrated filter, and thus the positive pressure gas can enter the integrated filter from the air nozzle 403.

[0046] In this embodiment, the first driving mechanism 401 can be a linear module, a cylinder, or other mechanisms that can realize driving the pressing plate 402 to rise and fall. This is relatively mature in the prior art and will not be limited and elaborated here.

[0047] In one embodiment, as Figure 6 shown, an elastic sealing suction cup 408 is installed at the docking end of the air nozzle 403 and the inlet 305 of the integrated filter. When the first driving mechanism 401 drives the pressing plate 402 to move downward towards the integrated filter and the air nozzle 403 is docked with the integrated filter, the elastic sealing suction cup 408 can contact the upper end surface of the liquid storage box 303, thereby preventing the positive pressure gas coming out of the air nozzle 403 from leaking.

[0048] In one embodiment, the integrated filter is installed on the bracket 302, and this bracket 302 is connected to the second driving mechanism 308. The second driving mechanism 308 can drive the bracket 302 to perform a horizontal linear motion. Through the driving of the second driving mechanism 308, the bracket 302 extends out of the box body 101, or the bracket 302 retracts into the box body 101.

[0049] In this embodiment, the second driving mechanism 308 can be a linear module, a cylinder, or other mechanisms that can realize driving the bracket 302 to extend out of the box body 101 or retract into the box body 101. This is relatively mature in the prior art and will not be limited and elaborated here.

[0050] In one embodiment, the test tube supporting mechanism includes a tray 203 and a third driving mechanism 204 connected to the tray 203. The third driving mechanism 204 can be used to drive the tray 203 to perform a horizontal linear motion. Through the driving of the third driving mechanism 204, the tray 203 extends out of the box body 101, or the tray 203 retracts into the box body 101.

[0051] In this embodiment, the third driving mechanism 204 can be a linear module, a cylinder, or other mechanisms that can realize driving the tray 203 to extend out of the box body 101 or retract into the box body 101. This is relatively mature in the prior art and will not be limited and elaborated here.

[0052] In one embodiment, as Figure 7 shown, the tray 203 is detachably connected with a test tube rack 202, and the test tube rack 202 is used for storing test tubes 201. Since it is usually necessary to move a batch of test tubes 201, making the test tube rack 202 detachably installed on the tray 203, multiple test tubes 201 can be moved more conveniently by moving the test tube rack 202.

[0053] In this embodiment, the test tube rack 202 can be fixed by bolts 205 installed on the tray 203, and the test tube rack 202 is provided with threaded holes corresponding to the bolts 205. The test tube rack 202 can also be detachably connected to the tray 203 in other ways, which is relatively mature in the prior art and will not be limited and elaborated here.

[0054] In one embodiment, as Figure 8 shown, the second driving mechanism 308 and the third driving mechanism 204 are respectively located on the side of the box body 101, and the first driving mechanism 401 is located on the side of the box body 401. Configuring the first driving mechanism 401, the second driving mechanism 308, and the third driving mechanism 204 in this way can make the interior of the device more compact, save space, and enable the volume of the box body 101 to be minimized as much as possible.

[0055] In one embodiment, the second driving mechanism 308 is a pressing type elastic self-locking mechanism, which is located on both sides of the box body 101. Press the bracket 302, and the bracket 302 can extend out of the box body 101. Then press the bracket 302 to make it retract into the box body 101, and the bracket 302 can be fixed. The pressing type elastic self-locking mechanism is relatively mature in the prior art and will not be elaborated here.

[0056] In this embodiment, the third driving mechanism 204 is located at the bottom of the box body 101, and the first driving mechanism 401 is located on the back of the box body 401.

[0057] In one embodiment, as Figure 4 shown, the bracket 302 is connected to the connection frame 301 through a buffer mechanism 304, and the connection frame 301 is connected to the second driving mechanism 308. When the nozzle 403 presses down on the integrated filter, the buffer mechanism 304 can play a buffering role to prevent the bracket 302 from being damaged due to the downward pressure.

[0058] The buffer mechanism 304 can be a spring or other mechanisms that can achieve the buffering function, which is relatively mature in the prior art and will not be elaborated and limited here.

[0059] In one embodiment, as Figure 4As shown in the figure, a limiting mechanism is provided between the bracket 302 and the pressing plate 402. Through this limiting mechanism, the distance that the pressing plate 402 presses down can be limited, avoiding damage to the bracket 302 and even the integrated filter due to excessive downward movement of the pressing plate 402.

[0060] Specifically, the limiting mechanism includes a plug rod 404 fixedly connected to the pressing plate 402. A threaded portion 405 is provided at the lower end of the plug rod 404, and a nut 406 is installed on the threaded portion 405. A jack 407 adapted to the plug rod 404 is provided on the connecting frame 301. By turning the nut 406, the position of the nut 406 can be adjusted. Since the nut 406 cannot pass through the jack 407, when the pressing plate 402 moves downward, the lower end of the plug rod 404 first inserts into the jack 407. When the nut 406 contacts the connecting frame 301, the plug rod 404 cannot move further downward, that is, the pressing plate 402 cannot move further downward.

[0061] In one embodiment, as Figure 5 shown, a filter 307 is installed at the liquid outlet 306 of the liquid storage box 303. When no positive pressure gas is introduced, the liquid in the liquid storage box 303 cannot pass through the filter membrane in the filter 307. When positive pressure gas is introduced into the inlet 305, the liquid in the liquid storage box 303 passes through the filter membrane of the filter 307 and flows out from the outlet of the filter 307, falling into the lower test tube 201.

[0062] In one embodiment, as Figure 1 shown, universal wheels 102 are provided at the bottom of the box body 101, so that this device can be moved more conveniently. A display screen 103 connected to the controller can also be installed on the box body 101, so as to display the opening and closing states of the solenoid valves 105, as well as the number of air pumps 104 and solenoid valves 105 that are opened. This is relatively mature in the prior art and will not be elaborated here.

[0063] The utility model can also be installed with an ultraviolet sterilization mechanism to exert the ultraviolet sterilization function. Moreover, the utility model can be installed with a signal transmission module to realize the transmission of liquid blowing information.

[0064] When this device is in use, extend the bracket 302 and the tray 203 out of the box body 101, place the integrated filter and the test tube rack 202 with the test tube 201, then retract the bracket 302 and the tray 203 into the box body 101, and then make the pressing plate 402 move towards the integrated filter below, so that the air nozzle 403 is docked with the integrated filter. Control the corresponding solenoid valve 105 to open according to the number of integrated filters to be blown with liquid, and perform positive pressure liquid blowing. After liquid blowing, the integrated filter sample directly discharges the internal reagent into the test tube 201. After liquid blowing is completed, the bracket 302 and the tray 203 can be extended out of the box body 101 to take out the integrated filter and the test tube 201.

[0065] The above has schematically described the present utility model and its implementation manners. This description is not restrictive. What is shown in the drawings is only one of the implementation manners of the present utility model, and the actual structure is not limited thereto. Therefore, if those of ordinary skill in the art are inspired by it and, without departing from the gist of the creation of the present utility model, design similar structural modes and embodiments to this technical solution without creative efforts, they shall fall within the protection scope of the present utility model.

Claims

1. An environmental DNA blowing device, comprising a housing (101), characterized in that: The box (101) is provided with: A plurality of integrated filters, each of which is provided with a liquid storage box (303) for storing liquid; A test tube supporting mechanism, the test tube supporting mechanism is used to support the test tube (201) and place the test tube (201) below the integrated filter; A gas power mechanism is used to provide positive pressure gas to the inlet (305) of the integrated filter, so that the liquid in the liquid storage box (303) enters the test tube (201).

2. The environmental DNA blow-off device according to claim 1, characterized in that: The gas power mechanism comprises N1 air pumps (104), each air pump (104) is connected to N2 solenoid valves (105), and each solenoid valve (105) is connected to a gas nozzle (403); The air nozzle (403) is used to connect with the inlet (305) of the integrated filter; N1 is a positive integer, and N1≥1; N2 is a positive integer, and N2≥1.

3. The environmental DNA blow-off device according to claim 2, characterized in that: The air nozzle (403) is installed on a pressure plate (402), and the pressure plate (402) is connected to the first driving mechanism (401); The first driving mechanism (401) is used to drive the pressing plate (402) to perform vertical linear motion.

4. The environmental DNA blow-off device according to claim 2, characterized in that: An elastic sealing suction cup (408) is installed at the butt end between the air nozzle (403) and the inlet (305) of the integrated filter.

5. The environmental DNA blow-off device according to claim 3, characterized in that: The integrated filter is mounted on a bracket (302), and the bracket (302) is connected to a second driving mechanism (308), and the second driving mechanism (308) is used to drive the bracket (302) to perform transverse linear motion; The test tube supporting mechanism comprises a tray (203) and a third driving mechanism (204) connected to the tray (203), wherein the third driving mechanism (204) is used to drive the tray (203) to perform transverse linear motion.

6. The environmental DNA blow-off device according to claim 5, characterized in that: The tray (203) is detachably connected to a test tube rack (202), and the test tube rack (202) is used to store test tubes (201).

7. The environmental DNA blow-off device according to claim 5, characterized in that: The second driving mechanism (308) and the third driving mechanism (204) are respectively located on the sides of the box body (101); The first driving mechanism (401) is located on the side of the box body (101).

8. The environmental DNA blow-off device according to claim 5, characterized in that: The second driving mechanism (308) is a press-type elastic self-locking mechanism, which is located on both sides of the box body (101); The third driving mechanism (204) is located at the bottom of the box (101); The first driving mechanism (401) is located on the back side of the box body (101).

9. The environmental DNA blow-off device according to claim 8, characterized in that: The bracket (302) is connected to the connection frame (301) via a buffer mechanism (304), and the connection frame (301) is connected to a second driving mechanism (308).

10. The environmental DNA blow-off device according to claim 9, characterized in that: A limiting mechanism is provided between the bracket (302) and the pressing plate (402); The limiting mechanism comprises an insertion rod (404) fixedly connected to the pressure plate (402), the lower end of the insertion rod (404) is provided with a threaded portion (405), and a nut (406) is installed on the threaded portion (405); the connection frame (301) is provided with a socket (407) adapted to the insertion rod (404).

11. The environmental DNA blow-off device according to any one of claims 1 to 10, characterized in that: The liquid outlet (306) of the liquid storage box (303) is installed with a filter (307). When positive pressure gas is introduced into the inlet (305), the liquid in the liquid storage box (303) passes through the filter membrane of the filter (307) and flows out from the outlet of the filter (307).

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