Microbial strain inoculator

By designing a microbial seed inoculation device, using the combination of telescopic rod and slide chute, the inoculation ring is kept away from the Petri dish when marking, which solves the contamination and error problems caused by the contact between the inoculation ring and the Petri dish, and improves the accuracy of the inoculation.

CN222907902UActive Publication Date: 2025-05-27ZHEJIANG RIFULAI AGRI TECH CO LTD
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Patent Information

Application Number
CN202421419331.4
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2024-06-19
Publication Date
2025-05-27
Estimated Expiration
2034-06-19

AI Technical Summary

Technical Problem

In the existing microbial inoculation methods, the inoculation loop is prone to contact the bottom of the Petri dish when scribed, resulting in contamination and errors in the inoculation results.

Method used

A microbial bacterial seed inoculation device is designed. Through the combination of a connecting frame, a chute and a telescopic rod, the inoculation ring can be marked on the surface of the Petri dish through the length variation of the first telescopic rod and the second telescopic rod to ensure that the inoculation ring is always kept away from the Petri dish.

Benefits of technology

It effectively reduces the contact between the Petri dish and the inoculation ring, reduces the risk of contamination, and improves the accuracy of inoculation.

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Abstract

The utility model belongs to the field of microbial inoculation, and particularly relates to a microbial strain inoculator which comprises a connecting frame, first sliding grooves are symmetrically formed in the inner wall of the connecting frame. A first telescopic rod is slidably connected into the first sliding groove. Second sliding grooves are symmetrically formed in the inner wall of the connecting frame. A second telescopic rod is slidably connected into the second sliding groove. The ends of the adjacent first telescopic rods are fixedly connected with fixing rings. The fixing ring is fixedly connected with the pair of second telescopic rods; the middle part of the connecting frame is symmetrically and fixedly connected with connecting plates; the end part of the connecting plate is fixedly connected with a fixing mechanism; after the inoculating loop is fixed by the fixing ring, scribing can be performed on the surface of the culture dish through the length change of the first telescopic rod and the second telescopic rod, so that the inoculating loop can always keep a distance from the culture dish in the scribing process, pollution to the culture dish due to contact with the inoculating loop is reduced, and the inoculating accuracy of the device is improved.
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Description

Technical Field

[0001] The utility model relates to the field of microorganism inoculation, in particular to a microorganism strain inoculator. Background Technique

[0002] Microorganism inoculation is the process of inoculating microorganisms onto an artificial culture medium suitable for their growth and reproduction or into a living organism. It is mainly used for the pure culture of microorganisms, observing certain characteristics of microorganisms, preserving strains, and conducting related microbiological research.

[0003] There are many methods of microorganism inoculation, specifically including the following: streak plate isolation method, slant inoculation method, stab inoculation method, liquid inoculation method, spread plate inoculation method.

[0004] When inoculating existing microorganisms, the streak plate method is usually used for inoculation. It is found in use and observation that the inoculation loop will come into contact with the culture dish during streaking, which will contaminate the bottom of the culture dish, and then cause errors in the inoculation results.

[0005] Therefore, a microorganism strain inoculator is proposed for the above problems. Content of the Utility Model

[0006] In order to make up for the deficiencies of the prior art and solve at least one technical problem proposed in the background technique.

[0007] The technical solution adopted by the utility model to solve its technical problems is as follows: A microorganism strain inoculator of the utility model includes a connecting frame; first sliding grooves are symmetrically opened on the inner wall of the connecting frame; a first telescopic rod is slidably connected inside the first sliding grooves; second sliding grooves are symmetrically opened on the inner wall of the connecting frame; a second telescopic rod is slidably connected inside the second sliding grooves; the ends of adjacent first telescopic rods are fixedly connected with fixing rings; the fixing rings and a pair of second telescopic rods are also fixedly connected; connecting plates are symmetrically and fixedly connected to the middle of the connecting frame; fixing mechanisms are fixedly connected to the ends of the connecting plates; after the inoculation loop is fixed by the fixing ring, it can streak on the surface of the culture dish through the length change of the first telescopic rod and the second telescopic rod, so that the inoculation loop can always keep a distance from the culture dish during streaking, reduce the contamination of the culture dish caused by contact with the inoculation loop, and improve the inoculation accuracy of the device.

[0008] Preferably, a first elastic cloth is fixedly connected to the top of the fixing ring; first connecting rods are symmetrically and fixedly connected to the top of the connecting frame; a guiding plate is fixedly connected between adjacent first connecting rods; the guiding plate and the first elastic cloth are also fixedly connected; by setting the guiding plate, the inoculation loop will pass through the guiding plate and slide along the surface of the guiding plate to the fixing ring before entering the fixing ring, realizing the guiding of the inoculation loop by the device and reducing the difficulty of fixing the inoculation loop by the fixing ring.

[0009] Preferably, a plurality of sponges are fixedly connected to the inner wall of the first elastic cloth; the sponges are located between the guiding plate and the fixing ring; by arranging the sponges, the sponges will absorb the sweat on the surface of the inoculation loop, reduce the pollution sources on the surface of the inoculation loop, and reduce the interference of external factors on the inoculation process.

[0010] Preferably, the fixing mechanism includes clamping plates; a plurality of fixing grooves are formed in the middle of the clamping plates; a first spring is fixedly connected to the middle of the fixing grooves; a top plate is fixedly connected to the end of the first spring; a second elastic cloth is fixedly connected between adjacent top plates; when the culture dish presses on the top plate, the top plate will, under the elastic force of the first spring, closely adhere to the surface of the culture dish together with the second elastic cloth, realizing the fixation of the device to culture dishes of different shapes and different sizes, and improving the flexibility of the device in fixing culture dishes of different shapes and different sizes.

[0011] Preferably, a suction cup is fixedly connected to the middle of the top plate; the suction cups are arranged in an arc array; by arranging the suction cups, after being pressed by the culture dish, the suction cups will adsorb the surface of the culture dish under the action of atmospheric pressure, increasing the contact area between the device and the culture dish, and enhancing the stability of the device in fixing the culture dish.

[0012] Preferably, a plurality of second springs are fixedly connected to the middle of the top plate; the ends of adjacent second springs are fixedly connected with a pressing ring; the pressing ring is arranged corresponding to the suction cup; through the combined action of the pressing ring and the second spring; after being pressed by the culture dish, the pressing ring will press the middle of the suction cup under the elastic force of the second spring, reducing the influence of external air on the suction cup, improving the airtightness of the suction cup itself, and enhancing the adsorption effect of the suction cup on the culture dish.

[0013] Preferably, a rubber pad is fixedly connected to the middle of the second elastic cloth; the surface of the rubber pad is arc-shaped; when the second elastic cloth contacts the culture dish, the rubber pad will also contact the culture dish. Because the friction coefficient of the surface of the rubber pad is larger, the friction force between the device and the culture dish will be increased.

[0014] The beneficial effects of the present utility model are as follows:

[0015] 1. For the microorganism strain inoculator of the present utility model, after the inoculation loop is fixed by the fixing ring, it can draw lines on the surface of the culture dish through the length change of the first telescopic rod and the second telescopic rod, so that the inoculation loop can always keep a distance from the culture dish during the line drawing process, reducing the pollution of the culture dish caused by contact with the inoculation loop, and improving the inoculation accuracy of the device.

[0016] 2. For the microorganism strain inoculator of the present utility model, by arranging the guiding plate, before entering the fixing ring, the inoculation loop will pass through the guiding plate and slide along the surface of the guiding plate to the fixing ring, realizing the guiding of the inoculation loop by the device and reducing the difficulty of fixing the inoculation loop by the fixing ring. Description of the Drawings

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0018] Figure 1 It is the main body schematic diagram of the present invention;

[0019] Figure 2 It is the structural schematic diagram of the guiding plate in the present invention;

[0020] Figure 3 It is the structural schematic diagram of the clamping plate in the present invention;

[0021] Figure 4 It is the structural schematic diagram of the pressure ring in the present invention.

[0022] In the figure: 1, connecting frame; 12, first sliding groove; 13, second sliding groove; 14, first telescopic rod; 15, second telescopic rod; 16, fixing ring; 17, connecting plate; 18, fixing mechanism; 2, first elastic cloth; 22, guiding plate; 23, first connecting rod; 3, sponge; 4, clamping plate; 42, fixing groove; 43, first spring; 44, top plate; 45, second elastic cloth; 5, suction cup; 6, pressure ring; 62, second spring; 7, rubber pad. Specific embodiments

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0024] The following gives specific embodiments.

[0025] Please refer to Figure 1As shown in the figure, a microorganism strain inoculator according to an embodiment of the present utility model includes a connecting frame 1; first sliding grooves 12 are symmetrically formed in the inner wall of the connecting frame 1; a first telescopic rod 14 is slidably connected inside the first sliding grooves 12; second sliding grooves 13 are symmetrically formed in the inner wall of the connecting frame 1; a second telescopic rod 15 is slidably connected inside the second sliding grooves 13; the ends of adjacent first telescopic rods 14 are fixedly connected with fixing rings 16; the fixing rings 16 and a pair of second telescopic rods 15 are fixedly connected; connecting plates 17 are symmetrically and fixedly connected to the middle of the connecting frame 1; fixing mechanisms 18 are fixedly connected to the ends of the connecting plates 17; during operation, after the inoculation loop is dipped in the bacterial liquid, it can be sleeved inside the fixing ring 16, and then the inoculation loop will be fixed by the fixing ring 16. Subsequently, the moving device makes the fixing mechanism 18 contact the culture dish and fixes the fixing mechanism 18. At this time, the distance between the inoculation loop and the culture dish will always remain the same. Then, the staff can move the inoculation loop around to make the bacterial liquid evenly distributed in the culture dish. When the inoculation loop moves, it will drive the fixing ring 16 to move together. When the fixing ring 16 moves along the second telescopic rod 15, the lengths of the two second telescopic rods 15 will change and cause the first telescopic rod 14 to slide along the first sliding groove 12. When the fixing ring 16 moves along the first telescopic rod 14, the lengths of the two first telescopic rods 14 will also change and the second telescopic rod 15 will also slide along the second sliding groove 13; after the inoculation loop is fixed by the fixing ring 16, it can draw lines on the surface of the culture dish through the length change of the first telescopic rod 14 and the second telescopic rod 15, so that the inoculation loop can always keep a distance from the culture dish during the line drawing process, reducing the pollution of the culture dish caused by contact with the inoculation loop and improving the inoculation accuracy of the device.

[0026] Please refer to Figure 1 and Figure 2 As shown in the figure, a first elastic cloth 2 is fixedly connected to the top of the fixing ring 16; first connecting rods 23 are symmetrically and fixedly connected to the top of the connecting frame 1; a guiding plate 22 is fixedly connected between adjacent first connecting rods 23; the guiding plate 22 and the first elastic cloth 2 are fixedly connected; before the inoculation loop is sleeved in the fixing ring 16, the side wall of the inoculation loop will contact the guiding plate 22 and slide down along the surface of the guiding plate 22 into the first elastic cloth 2. Subsequently, the inoculation loop will enter the fixing ring 16 along the first elastic cloth 2 and be fixed by the fixing ring 16; by setting the guiding plate 22, the inoculation loop will pass through the guiding plate 22 and slide along the surface of the guiding plate 22 to the fixing ring 16 before entering the fixing ring 16, realizing the guiding of the inoculation loop by the device and reducing the difficulty of fixing the inoculation loop by the fixing ring 16.

[0027] Please refer to Figure 2As shown, a plurality of sponges 3 are fixedly connected to the inner wall of the first elastic cloth 2; the sponges 3 are located between the guiding plate 22 and the fixing ring 16; when the inoculation loop passes through the first elastic cloth 2, the inoculation loop will come into contact with the sponges 3, and the sponges 3 will absorb the sweat on the surface of the inoculation loop, reducing the interference of sweat on the inoculation process; by providing the sponges 3, the sponges 3 will absorb the sweat on the surface of the inoculation loop, reducing the pollution source on the surface of the inoculation loop and reducing the interference of external factors on the inoculation process.

[0028] Please refer to Figure 3 As shown, the fixing mechanism 18 includes a clamping plate 4; a plurality of fixing grooves 42 are formed in the middle of the clamping plate 4; a first spring 43 is fixedly connected to the middle of the fixing groove 42; a top plate 44 is fixedly connected to the end of the first spring 43; a second elastic cloth 45 is fixedly connected between adjacent top plates 44; when the clamping plate 4 contacts the culture dish, the top plate 44 will contact the culture dish and be squeezed by the culture dish, and the top plate 44 will transmit the pressure to the first spring 43 and make the first spring 43 in a compressed state. Subsequently, the first spring 43 will release its elastic potential energy and make the top plate 44 squeeze the surface of the culture dish. When the top plate 44 moves, it will also drive the second elastic cloth 45 to fit the surface of the culture dish; when the culture dish squeezes the top plate 44, the top plate 44 will, under the elastic force of the first spring 43, closely adhere to the surface of the culture dish together with the second elastic cloth 45, realizing the fixation of the device to culture dishes of different shapes and different sizes and improving the flexibility of the device to fix culture dishes of different shapes and different sizes.

[0029] Please refer to Figure 3 and Figure 4 As shown, a suction cup 5 is fixedly connected to the middle of the top plate 44; the suction cups 5 are arranged in an arc array; when the culture dish contacts the top plate 44, the culture dish will also contact the suction cups 5 and squeeze the suction cups 5. After the suction cups 5 are squeezed, the air inside will be discharged, and under the action of atmospheric pressure, the suction cups 5 will adsorb the surface of the culture dish; by providing the suction cups 5, after the suction cups 5 are squeezed by the culture dish, they will adsorb the surface of the culture dish under the action of atmospheric pressure, increasing the contact area between the device and the culture dish and enhancing the stability of the device to fix the culture dish.

[0030] Please refer to Figure 4As shown, a plurality of second springs 62 are fixedly connected to the middle of the top plate 44; the ends of adjacent second springs 62 are fixedly connected with a pressure ring 6; the pressure ring 6 is arranged corresponding to the suction cup 5; when the culture dish presses the suction cup 5, the culture dish will also press the pressure ring 6. After being pressed, the pressure ring 6 will transmit the pressure to the second spring 62 and make the second spring 62 in a compressed state. After being compressed, the second spring 62 will release elastic potential energy and apply an elastic force to the pressure ring 6. After receiving the elastic force of the second spring 62, the pressure ring 6 will press the middle part of the suction cup 5; through the cooperation of the pressure ring 6 and the second spring 62; after being pressed by the culture dish, the pressure ring 6 will press the middle part of the suction cup 5 under the action of the elastic force of the second spring 62, reducing the influence of external air on the suction cup 5, improving the airtightness of the suction cup 5 itself, and enhancing the adsorption effect of the suction cup 5 on the culture dish.

[0031] Please refer to Figure 3 As shown, a rubber pad 7 is fixedly connected to the middle of the second elastic cloth 45; the surface of the rubber pad 7 is an arc structure; when the second elastic cloth 45 contacts the culture dish, the rubber pad 7 will also contact the culture dish. Because the friction coefficient of the surface of the rubber pad 7 is larger, the friction force between the device and the culture dish will be increased.

[0032] Working principle: After the inoculation loop is dipped in the bacterial liquid, it can be sleeved inside the fixing ring 16. Subsequently, the inoculation loop will be fixed by the fixing ring 16. Then, the moving device makes the fixing mechanism 18 contact the culture dish and fixes the fixing mechanism 18. At this time, the distance between the inoculation loop and the culture dish will always remain the same. Then, the staff can move the inoculation loop around to make the bacterial liquid evenly distributed in the culture dish. When the inoculation loop moves, it will drive the fixing ring 16 to move together. When the fixing ring 16 moves along the second telescopic rod 15, the lengths of the two second telescopic rods 15 will change and cause the first telescopic rod 14 to slide along the first chute 12. When the fixing ring 16 moves along the first telescopic rod 14, the lengths of the two first telescopic rods 14 will also change and the second telescopic rod 15 will also slide along the second chute 13; Before the inoculation loop is sleeved in the fixing ring 16, the side wall of the inoculation loop will contact the guiding plate 22 and slide down along the surface of the guiding plate 22 into the interior of the first elastic cloth 2. Subsequently, the inoculation loop will enter the fixing ring 16 along the first elastic cloth 2 and be fixed by the fixing ring 16; When the inoculation loop passes through the first elastic cloth 2, the inoculation loop will contact the sponge 3, and the sponge 3 will absorb the sweat on the surface of the inoculation loop, reducing the interference of sweat on the inoculation process; When the clamping plate 4 contacts the culture dish, the top plate 44 will contact the culture dish and be squeezed by the culture dish. The top plate 44 will transmit the pressure to the first spring 43 and make the first spring 43 in a compressed state. Subsequently, the first spring 43 will release its elastic potential energy and make the top plate 44 squeeze the surface of the culture dish. When the top plate 44 moves, it will also drive the second elastic cloth 45 to fit the surface of the culture dish; When the culture dish contacts the top plate 44, the culture dish will also contact the suction cup 5 and squeeze the suction cup 5. After the suction cup 5 is squeezed, the air inside will be discharged, and under the action of atmospheric pressure, the suction cup 5 will adsorb the surface of the culture dish; When the culture dish squeezes the suction cup 5, the culture dish will also squeeze the pressing ring 6. After the pressing ring 6 is squeezed, it will transmit the pressure to the second spring 62 and make the second spring 62 in a compressed state. After the second spring 62 is compressed, it will release its elastic potential energy and apply an elastic force to the pressing ring 6. After the pressing ring 6 receives the elastic force of the second spring 62, it will squeeze the middle part of the suction cup 5; When the second elastic cloth 45 contacts the culture dish, the rubber pad 7 will also contact the culture dish. Because the friction coefficient of the surface of the rubber pad 7 is larger, it will increase the friction between the device and the culture dish.

[0033] The above shows and describes the basic principle, main features and advantages of the present utility model. Those skilled in the art of this industry should understand that the present utility model is not limited by the above embodiments. What is described in the above embodiments and the specification only illustrates the principle of the present utility model. Without departing from the spirit and scope of the present utility model, the present utility model will have various changes and improvements, and these changes and improvements all fall within the scope of the present utility model claimed.

Claims

1. A microbial strain inoculator, comprising a connecting frame (1), characterized in that: The inner wall of the connecting frame (1) is symmetrically provided with a first sliding groove (12); a first telescopic rod (14) is slidably connected inside the first sliding groove (12); a second sliding groove (13) is symmetrically provided on the inner wall of the connecting frame (1); a second telescopic rod (15) is slidably connected inside the second sliding groove (13); a fixing ring (16) is fixedly connected to the ends of adjacent first telescopic rods (14); the fixing ring (16) and a pair of second telescopic rods (15) are in a fixed connection relationship; a connecting plate (17) is symmetrically fixedly connected to the middle of the connecting frame (1); a fixing mechanism (18) is fixedly connected to the ends of the connecting plate (17).

2. A microbial strain inoculator according to claim 1, characterized in that: The top of the fixing ring (16) is fixedly connected to a first elastic cloth (2); the top of the connecting frame (1) is symmetrically fixedly connected to first connecting rods (23); guide plates (22) are fixedly connected between adjacent first connecting rods (23); and the guide plates (22) and the first elastic cloth (2) are both in a fixed connection relationship.

3. A microbial strain inoculator according to claim 2, characterized in that: A plurality of sponges (3) are fixedly connected to the inner wall of the first elastic cloth (2); the sponges (3) are located between the guide plate (22) and the fixing ring (16).

4. A microbial strain inoculator according to claim 3, characterized in that: The fixing mechanism (18) comprises a clamping plate (4); a plurality of fixing grooves (42) are provided in the middle of the clamping plate (4); a first spring (43) is fixedly connected to the middle of the fixing groove (42); a top plate (44) is fixedly connected to the end of the first spring (43); and a second elastic cloth (45) is fixedly connected between adjacent top plates (44).

5. A microbial strain inoculator according to claim 4, characterized in that: A suction cup (5) is fixedly connected to the middle of the top plate (44); the suction cup (5) is arranged in an arc-shaped array.

6. A microbial strain inoculator according to claim 5, characterized in that: A plurality of second springs (62) are fixedly connected to the middle of the top plate (44); pressure rings (6) are fixedly connected to the ends of adjacent second springs (62); and the pressure rings (6) and the suction cups (5) are arranged correspondingly.