Portable constant-temperature nucleic acid amplification analyzer control circuit

Abstract

The utility model provides a portable constant-temperature nucleic acid amplification analyzer control circuit, which comprises a power supply interface, a PD photodiode, an LED excitation light source, a linear voltage regulator, an operational amplification circuit, a first NMOS tube, a voltage division circuit, a second NMOS tube and an MCU main control chip, the power supply interface supplies power to the MCU main control chip, and the power supply interface supplies power to the MCU main control chip. The linear voltage regulator, the operational amplifier circuit, the first NMOS tube, the voltage division circuit and the second NMOS tube are all electrically connected with the MCU main control chip, the LED excitation light source is electrically connected with the first NMOS tube, the PD photodiode is electrically connected with the operational amplifier circuit, and the second NMOS tube is electrically connected with the heating film. According to the control circuit of the portable constant-temperature nucleic acid amplification analyzer, the complex design of an existing instrument is simplified, the temperature accuracy, the temperature stability and the temperature uniformity can be ensured, and the stability and the sensitivity of fluorescence detection of the instrument can also be ensured.

Description

Technical Field

[0001] The utility model relates to the field of constant temperature circuit control, and particularly relates to a control circuit for a portable constant temperature nucleic acid amplification analyzer. Background Art

[0002] The constant temperature nucleic acid amplification analyzer is one of the commonly used instruments in gene amplification detection, and can be used for judging genetic disease maps, diagnosing infectious diseases, identifying target genes, paternity testing, etc. in a test sample. With the rapid development of molecular biology technology, diagnostic methods based on nucleic acid detection have been established in large numbers and widely applied to laboratory detection of human diseases. The reaction process of the constant temperature nucleic acid amplification technology requires that the nucleic acid reagent always be maintained at a constant temperature, and different active enzymes and their specific primers are added to achieve the purpose of rapid nucleic acid amplification. In existing products, the constant temperature amplification analyzer is bulky, the operation process is cumbersome, the instrument circuit design is complex, and the cost is high.

[0003] In existing products, the constant temperature nucleic acid amplification analyzer is bulky and not portable, the operation process is cumbersome, the instrument circuit design is complex, and the cost is high. There is an urgent need to design a control circuit for a low-cost portable constant temperature nucleic acid amplification analyzer, which not only needs to ensure the temperature control performance of the instrument, such as temperature accuracy, stability, uniformity, etc., while ensuring low cost, but also needs to ensure the stability and sensitivity of the fluorescence acquisition signal of the instrument. Summary of the Utility Model

[0004] The main purpose of the utility model is to propose a control circuit for a portable constant temperature nucleic acid amplification analyzer, which overcomes the above technical problems.

[0005] In order to achieve the above purpose, the utility model proposes the following technical solutions:

[0006] A control circuit for a portable constant temperature nucleic acid amplification analyzer includes: a power interface, a nucleic acid reagent reaction position, a PD photodiode, an LED excitation light source, a linear voltage regulator, an operational amplifier circuit, a first NMOS transistor, a voltage dividing circuit, a second NMOS transistor, and an MCU main control chip;

[0007] The power interface is electrically connected to one end of the linear voltage regulator, and the other end of the linear voltage regulator is electrically connected to the MCU main control chip;

[0008] The MCU main control chip is electrically connected to the gate of the first NMOS transistor, the drain of the first NMOS transistor is electrically connected to one end of the LED excitation light source, the other end of the LED excitation light source is electrically connected to one end of a resistor, the other end of the resistor is connected to the power supply voltage, and the source of the first NMOS transistor is connected to the ground;

[0009] The PD photodiode is electrically connected to the operational amplifier circuit, and the operational amplifier circuit is electrically connected to the MCU main control chip;

[0010] The gate of the MCU main control chip is electrically connected to the gate of the second NMOS transistor. The drain of the second NMOS transistor is electrically connected to one end of the heating film, and the other end of the heating film is connected to the power supply voltage.

[0011] Furthermore, the circuit power supply is a DC power supply.

[0012] Furthermore, the power interface uses a USB interface.

[0013] Furthermore, the power interface uses a Type-C interface.

[0014] Furthermore, nucleic acid reagent reaction sites are provided on the heating film.

[0015] Furthermore, an NTC temperature sensor is provided at the nucleic acid reaction site. The NTC temperature sensor is electrically connected to a voltage dividing circuit, and the voltage dividing circuit is electrically connected to the MCU main control chip.

[0016] Furthermore, the MCU main control chip is also electrically connected to a Bluetooth module.

[0017] Furthermore, the MCU main control chip outputs a pulse width modulation signal to the fan to adjust the speed of the fan, thereby adjusting the cooling rate in real time and achieving the purpose of controlling the temperature of the nucleic acid reaction site.

[0018] Furthermore, mobile phones, PADs, and PC devices can communicate with the constant-temperature nucleic acid amplification analyzer wirelessly via Bluetooth. The device sends the temperature and fluorescence detection signal data collected at the reagent heating site to an interactive terminal for subsequent data processing.

[0019] Furthermore, the MCU main control chip is also electrically connected to the gate of the third NMOS transistor. The drain of the third NMOS transistor is connected to one end of the fan, and the other end of the fan is connected to the power supply voltage.

[0020] Through the control circuit of a portable constant-temperature nucleic acid amplification analyzer of the present utility model, the complex design of existing instruments is simplified. It can not only ensure temperature accuracy, temperature stability, and temperature uniformity, but also ensure the stability and sensitivity of fluorescence detection of the instrument. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The schematic diagrams forming a part of this application are used to provide a further understanding of the present utility model. The illustrative embodiments and descriptions of the present utility model are used to explain the present utility model and do not constitute an improper limitation of the present utility model. In the drawings:

[0022] Figure 1 The schematic diagram of the control circuit of a portable constant-temperature nucleic acid amplification analyzer of the present utility model is shown.

[0023] Among them, the above-mentioned drawings include the following reference numerals:

[0024] 1. Power interface; 2. Fan; 4. Nucleic acid reagent reaction site; 5. PD photodiode; 6. LED excitation light source; 7. Linear voltage regulator; 8. Operational amplifier circuit; 9. First NMOS transistor; 10. Voltage division circuit; 11. Second NMOS transistor; 12. Third NMOS transistor; 13. Bluetooth module; 14. MCU main control chip. Detailed implementation manners

[0025] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. The following description of at least one exemplary embodiment is actually only illustrative and in no way restricts the present invention and its application or use. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0026] It should be noted that the terms used herein are only for describing specific implementation manners and are not intended to limit the exemplary implementation manners according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "include" and / or "comprise" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0027] Unless otherwise specifically stated, the relative arrangements, numerical expressions, and numerical values of the components and steps described in these embodiments do not limit the scope of the present invention. At the same time, it should be understood that for the convenience of description, the dimensions of the various parts shown in the drawings are not drawn in actual proportional relationships. Technologies, methods, and devices known to those of ordinary skill in the relevant art may not be discussed in detail, but where appropriate, the technologies, methods, and devices should be regarded as part of the authorized specification. In all the examples shown and discussed here, any specific value should be interpreted as merely exemplary and not as a limitation. Therefore, other examples of the exemplary embodiments may have different values. It should be noted that: similar reference numerals and letters represent similar items in the following drawings. Therefore, once an item is defined in one drawing, it does not need to be further discussed in subsequent drawings.

[0028] Next, refer to Figure 1 , and make a further description of the present invention:

[0029] A control circuit for a portable constant-temperature nucleic acid amplification analyzer, comprising: a power interface 1, a PD photodiode 5, an LED excitation light source 6, a linear voltage regulator 7, an operational amplifier circuit 8, a first NMOS transistor 9, a voltage-dividing circuit 10, a second NMOS transistor 11, and an MCU main control chip 14;

[0030] The power interface 1 is electrically connected to one end of the linear voltage regulator 7, and the other end of the linear voltage regulator 7 is electrically connected to the MCU main control chip 14;

[0031] In this embodiment, the circuit power supply is a DC power supply with a voltage of 5V and a current that can support 2A. The power supply output is connected to the linear voltage regulator 7, and after the voltage is converted to 3.3V, it supplies power to the MCU main control chip 14.

[0032] The MCU main control chip 14 is electrically connected to the gate of the first NMOS transistor 9. The drain of the first NMOS transistor 9 is electrically connected to one end of the LED excitation light source 6. The other end of the LED excitation light source 6 is electrically connected to one end of a resistor, and the other end of the resistor is connected to the power supply voltage. The source of the first NMOS transistor 9 is connected to the ground; the PD photodiode 5 is electrically connected to the operational amplifier circuit 8, and the operational amplifier circuit 8 is electrically connected to the MCU main control chip 14; the MCU main control chip 14 is electrically connected to the gate of the second NMOS transistor 11. The drain of the second NMOS transistor 11 is electrically connected to one end of the heating film, and the other end of the heating film is connected to the power supply voltage.

[0033] The MCU main control chip 14 outputs a pulse width modulation signal to the heating film to adjust the heating rate of the heating film in real time.

[0034] In this embodiment, a nucleic acid reagent reaction site 4 is provided on the heating film.

[0035] When the MCU main control chip 14 outputs a high-level signal, the LED excitation light source 6 can be turned on. When the MCU main control chip 14 outputs a low-level signal, the LED excitation light source 6 is turned off. By adjusting the resistance value of the connecting resistor, the LED power can be adjusted. The LED excitation light emitted by the LED excitation light source 6 and reflected by the nucleic acid reagent reaction site can enter the PD photodiode 5. The current signal of the fluorescence collected by the PD photodiode 5 is converted into a voltage signal by the operational amplifier circuit 8 and transmitted to the MCU main control chip 14 to complete the acquisition of the fluorescence signal of the nucleic acid reagent reaction site.

[0036] In this embodiment, the power interface uses a USB interface.

[0037] In an alternative embodiment, the power interface uses a Type-C interface.

[0038] In this embodiment, an NTC temperature sensor is provided at the nucleic acid reaction site 4. The NTC temperature sensor is electrically connected to the voltage-dividing circuit 10, and the voltage-dividing circuit 10 is electrically connected to the MCU main control chip 14.

[0039] The NTC temperature sensor converts the temperature resistance signal value into an analog voltage signal through the voltage division circuit 10. The MCU main control chip 14 can obtain the real-time temperature of the current nucleic acid reagent reaction site after calculating the received signal. Through PID modulation, precise temperature control of the nucleic acid reagent reaction site can be achieved. The temperature adjustment range is 30 to 100 °C, the temperature accuracy can be controlled within ±0.1 °C, and the temperature stability can reach ±0.02 °C.

[0040] In this embodiment, the MCU main control chip 14 is also electrically connected to the Bluetooth module 13. Mobile phones, PADs, and PC devices can communicate with the constant temperature nucleic acid amplification analyzer through wireless Bluetooth to send the temperature and fluorescence detection signal data collected by the device at the reagent heating site to the interactive terminal for subsequent data processing.

[0041] In this embodiment, the MCU main control chip 14 outputs a pulse width modulation signal to the fan 2 to adjust the speed of the fan 2, thereby adjusting the cooling rate in real time to achieve the purpose of controlling the temperature of the nucleic acid reaction site.

[0042] In this embodiment, the MCU main control chip 14 is also electrically connected to the gate of the third NMOS transistor 12. The drain of the third NMOS transistor 12 is connected to one end of the fan 2, and the other end of the fan 2 is connected to the power supply voltage.

[0043] In addition, it should be noted that the use of terms such as "first" and "second" to limit components is only for the convenience of distinguishing the corresponding components. Without further declaration, the above terms have no special meaning, so they cannot be understood as limiting the protection scope of the present invention.

[0044] The above are only the preferred embodiments of the present invention and are not used to limit the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A portable constant temperature nucleic acid amplification analyzer control circuit, characterized in that: It comprises: a power supply interface (1), a PD photodiode (5), an LED excitation light source (6), a linear voltage regulator (7), an operational amplifier circuit (8), a first NMOS tube (9), a voltage divider circuit (10), a second NMOS tube (11), and an MCU main control chip (14); The power interface (1) is electrically connected to one end of the linear voltage regulator (7), and the other end of the linear voltage regulator (7) is electrically connected to the MCU main control chip (14); The MCU main control chip (14) is electrically connected to the gate of the first NMOS tube (9), the drain of the first NMOS tube (9) is electrically connected to one end of the LED excitation light source (6), the other end of the LED excitation light source (6) is electrically connected to one end of a resistor, the other end of the resistor is connected to a power supply voltage, and the source of the first NMOS tube (9) is connected to the ground; The PD photodiode (5) is electrically connected to the operational amplifier circuit (8), and the operational amplifier circuit (8) is electrically connected to the MCU main control chip (14); The MCU main control chip (14) is electrically connected to the gate of the second NMOS tube (11), the drain of the second NMOS tube (11) is electrically connected to one end of the heating film, and the other end of the heating film is connected to a power supply voltage.

2. A portable constant temperature nucleic acid amplification analyzer control circuit according to claim 1, characterized in that: The circuit power supply is a DC power supply.

3. A portable constant temperature nucleic acid amplification analyzer control circuit according to claim 1, characterized in that: The power interface (1) is a USB interface.

4. A portable constant temperature nucleic acid amplification analyzer control circuit according to claim 1, characterized in that: The power interface (1) adopts a Type-C interface.

5. A portable constant temperature nucleic acid amplification analyzer control circuit according to claim 4, characterized in that: A nucleic acid reagent reaction position (4) is arranged on the heating film.

6. A portable constant temperature nucleic acid amplification analyzer control circuit according to claim 5, characterized in that: The nucleic acid reaction site (4) is provided with an NTC temperature sensor, the NTC temperature sensor is electrically connected to the voltage divider circuit (10), and the voltage divider circuit (10) is electrically connected to the MCU main control chip (14).

7. A portable constant temperature nucleic acid amplification analyzer control circuit according to claim 1, characterized in that: The MCU main control chip (14) is also electrically connected to the Bluetooth module (13).

8. A portable constant temperature nucleic acid amplification analyzer control circuit according to claim 1, characterized in that: The MCU main control chip (14) outputs a pulse width modulation signal to the fan (2) to adjust the speed of the fan (2), thereby adjusting the cooling rate in real time to achieve the purpose of controlling the temperature of the nucleic acid reaction site.

9. A portable constant temperature nucleic acid amplification analyzer control circuit according to claim 7, characterized in that: Mobile phones, PADs, and PC devices can communicate with the constant temperature nucleic acid amplification analyzer via wireless Bluetooth. The device will send the collected temperature and fluorescence detection signal data of the reagent heating position to the interactive terminal for subsequent data processing.

10. A portable constant temperature nucleic acid amplification analyzer control circuit according to claim 8, characterized in that: The MCU main control chip (14) is also electrically connected to the gate of the third NMOS tube (12); the drain of the third NMOS tube (12) is connected to one end of the fan (2); and the other end of the fan (2) is connected to a power supply voltage.

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