Nucleic acid drug transmembrane transport analysis device
By designing a transmembrane transport analysis device for nucleic acid drugs, using liquid storage units and ultraviolet light detection technology, the problem of difficult to determine the transmembrane transport capacity of nucleic acid drugs is solved, and the accuracy of nucleic acid drugs is achieved is achieved, and the accuracy of drug efficacy and bioavailability evaluation is improved.
Patent Information
- Application Number
- CN202421112748.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-16
- Publication Date
- 2025-05-27
- Estimated Expiration
- 2034-05-16
AI Technical Summary
The prior art is difficult to effectively determine the transmembrane transport capacity of nucleic acid drugs, affecting the efficacy and bioavailability of drugs.
A nucleic acid drug transmembrane transport analysis device is designed, including a first liquid storage unit for storing drug-containing buffer and a second liquid storage unit for storing blank buffer. The biofilm is clamped through a sample clip and communicated with the liquid storage unit. It combines an ultraviolet light source and an ultraviolet light sensor to detect the light intensity changes, and realizes the permeability determination of the nucleic acid drug.
The device can accurately determine the permeability of nucleic acid drugs, solve the problem of difficult to determine the transmembrane transport capacity of nucleic acid drugs, and improve the accuracy of drug efficacy and bioavailability evaluation.
Smart Images
Figure CN222913417U_ABST
Abstract
Description
Technical Field
[0001] The present disclosure relates to the field of experimental equipment, and in particular to a nucleic acid drug transmembrane transport analysis device. Background Art
[0002] Nucleic acid drugs (nucleic acid drugs) are a class of molecular drugs that inhibit gene expression by sequence-specific binding to target genes. Most of them are composed of 18-30 nucleotide single-stranded or double-stranded chains and contain specific chemical modifications. They treat diseases by regulating the expression of disease genes.
[0003] The permeability of a drug refers to the ability of drug molecules to pass through the biological membrane barrier and achieve transmembrane transport. For nucleic acid drugs, determining the permeability (i.e. the ability of the drug to transport across the membrane) is the key to determining the efficacy and bioavailability of the drug. Utility Model Content
[0004] The present disclosure provides a nucleic acid drug transmembrane transport analysis device. The technical solution is as follows:
[0005] The present disclosure provides a nucleic acid drug transmembrane transport analysis device, the device comprising:
[0006] A first liquid storage unit for storing a drug-containing buffer, a second liquid storage unit for storing a blank buffer, a sample clamp for clamping a biofilm, two ultraviolet light sources, two ultraviolet sensors and a signal analysis unit;
[0007] The sample clamp has two cavities, which are separated by the biofilm, the first liquid storage unit is connected to one of the two cavities, the second liquid storage unit is connected to the other of the two cavities, one of the two ultraviolet light sources and one of the two ultraviolet light sensors are correspondingly arranged on both sides of the first liquid storage unit, the other of the two ultraviolet light sources and the other of the two ultraviolet light sensors are correspondingly arranged on both sides of the second liquid storage unit, and the signal analysis unit is electrically connected to the two ultraviolet light sensors respectively.
[0008] Optionally, the first liquid storage unit includes a first liquid storage tank and a first pipeline, and the second liquid storage unit includes a second liquid storage tank and a second pipeline;
[0009] The first liquid storage tank and the second liquid storage tank are arranged on the same horizontal plane, and the first pipeline connects the first liquid storage tank and one of the two cavities, and the second pipeline connects the second liquid storage tank and the other of the two cavities.
[0010] Optionally, the first pipe and the second pipe are transparent pipes, one of the two ultraviolet light sources and one of the two ultraviolet light sensors are correspondingly arranged on both sides of the first pipe, the other of the two ultraviolet light sources and the other of the two ultraviolet light sensors are correspondingly arranged on both sides of the second pipe, and the light emission directions of the two ultraviolet light sources are parallel.
[0011] Optionally, the first liquid storage unit includes a first liquid storage tank, and the second liquid storage unit includes a second liquid storage tank;
[0012] The first liquid storage tank is located above the second liquid storage tank, and the bottom of the first liquid storage tank is connected to one of the two cavities, and the top of the second liquid storage tank is connected to the other of the two cavities.
[0013] Optionally, the first liquid storage tank and the second liquid storage tank are transparent liquid storage tanks, one of the two ultraviolet light sources and one of the two ultraviolet light sensors are correspondingly arranged on both sides of the first liquid storage tank, the other of the two ultraviolet light sources and the other of the two ultraviolet light sensors are correspondingly arranged on both sides of the second liquid storage tank, and the light emission directions of the two ultraviolet light sources are parallel.
[0014] Optionally, the first pipe and the second pipe are U-shaped pipes.
[0015] Optionally, the first liquid storage unit has a first vent, the second liquid storage unit has a second vent, the first pipe is in communication with the first vent, and the second pipe is in communication with the second vent.
[0016] Optionally, the device further comprises a shell, and the first liquid storage unit, the second liquid storage unit, the sample clamp, the two ultraviolet light sources, the two ultraviolet sensors and the signal analysis unit are all located in the shell.
[0017] Optionally, the device further comprises a display unit, wherein the display unit is located outside the housing and is electrically connected to the signal analysis unit.
[0018] Optionally, the display unit is embedded in the outer wall of the shell.
[0019] The technical solution provided by the embodiments of the present disclosure brings the following beneficial effects:
[0020] In the nucleic acid drug transmembrane transport analysis device provided in the embodiment of the present disclosure, a first liquid storage unit for storing drug-containing buffer and a second liquid storage unit for storing blank buffer are provided, and the biofilm is clamped by a sample clamp so that the sample clamp forms two separated cavities on both sides of the biofilm, and the two cavities are respectively connected to the first liquid storage unit and the second liquid storage unit, thereby forming a nucleic acid drug transmembrane transport simulation experimental structure. Then, by cooperating with an ultraviolet light source and an ultraviolet light sensor, the light intensity change of ultraviolet light passing through the buffer in the liquid storage unit is detected, and the first liquid storage unit and the second liquid storage unit are detected by ultraviolet light, and the signal is output to the signal analysis unit, so that the signal analysis unit can determine the permeability of the nucleic acid drug based on the detection signal, that is, the determination of the nucleic acid drug transmembrane transport ability is achieved. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] In order to more clearly illustrate the technical solutions in the embodiments of the present disclosure, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present disclosure. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.
[0022] Figure 1 is a cross-sectional schematic diagram of a nucleic acid drug transmembrane transport analysis device provided by an embodiment of the present disclosure;
[0023] Figure 2 It is a side view of one side of a nucleic acid drug transmembrane transport analysis device provided by an embodiment of the present disclosure;
[0024] Figure 3 is a side view of the other side of a nucleic acid drug transmembrane transport analysis device provided by an embodiment of the present disclosure;
[0025] Figure 4 It is a structural block diagram of a nucleic acid drug transmembrane transport analysis device provided in an embodiment of the present disclosure. DETAILED DESCRIPTION
[0026] In order to make the objectives, technical solutions and advantages of the present disclosure more clear, the embodiments of the present disclosure will be further described in detail below with reference to the accompanying drawings.
[0027] Figure 1 is a cross-sectional schematic diagram of a nucleic acid drug transmembrane transport analysis device provided by an embodiment of the present disclosure. Figure 1 The device includes: a first liquid storage unit 101 for storing a drug-containing buffer solution, a second liquid storage unit 102 for storing a blank buffer solution, and a sample clamp 103 for clamping a biofilm.
[0028] Among them, the drug refers to a nucleic acid drug, the drug-containing buffer refers to a buffer containing a nucleic acid drug, and the drug-containing buffer is located in the first liquid storage unit 101, corresponding to the donor side; the blank buffer refers to a buffer that initially does not contain a nucleic acid drug, and the blank buffer is located in the second liquid storage unit 102, corresponding to the receptor side.
[0029] Figure 2 is a side view of one side of a nucleic acid drug transmembrane transport analysis device provided by an embodiment of the present disclosure, Figure 3 FIG. 2 is a side view of the other side of a nucleic acid drug transmembrane transport analysis device provided by an embodiment of the present disclosure. Figure 2 and Figure 3 The device also includes: two ultraviolet light sources 104 and two ultraviolet light sensors 105.
[0030] Figure 4 is a structural block diagram of a nucleic acid drug transmembrane transport analysis device provided by an embodiment of the present disclosure. Figure 4 , the device also includes: a signal analysis unit 106.
[0031] Combination Figures 1 to 4 The sample holder 103 has two cavities 131, and the two cavities 131 are separated by the biofilm C. The first liquid storage unit 101 is connected to one of the two cavities 131, and the second liquid storage unit 102 is connected to the other of the two cavities 131. One of the two ultraviolet light sources 104 and one of the two ultraviolet sensors 105 are correspondingly arranged on both sides of the first liquid storage unit 101, and the other of the two ultraviolet light sources 104 and the other of the two ultraviolet sensors 105 are correspondingly arranged on both sides of the second liquid storage unit 102, and the signal analysis unit 106 is electrically connected to the two ultraviolet sensors 105 respectively.
[0032] In the nucleic acid drug transmembrane transport analysis device provided in the embodiment of the present disclosure, a first liquid storage unit for storing drug-containing buffer and a second liquid storage unit for storing blank buffer are provided, and the biofilm is clamped by a sample clamp so that the sample clamp forms two separated cavities on both sides of the biofilm, and the two cavities are respectively connected to the first liquid storage unit and the second liquid storage unit, thereby forming a nucleic acid drug transmembrane transport simulation experimental structure. Then, by cooperating with an ultraviolet light source and an ultraviolet light sensor, the light intensity change of ultraviolet light passing through the buffer in the liquid storage unit is detected, and the first liquid storage unit and the second liquid storage unit are detected by ultraviolet light, and the signal is output to the signal analysis unit, so that the signal analysis unit can determine the permeability of the nucleic acid drug based on the detection signal, that is, the determination of the nucleic acid drug transmembrane transport ability is achieved.
[0033] In the embodiment of the present disclosure, the signal analysis unit 106 may be a processor, a programmable controller, a single-chip microcomputer or other devices.
[0034] The signal analysis unit 106 converts the received signals of the two sensors into the permeability of the nucleic acid drug. For example, the signal analysis unit 106 determines the absorbance value of the buffer solution based on the light intensity at a wavelength of 260 nm, and then determines the drug content of the buffer solution and the drug permeability. For nucleic acid drugs, since the base part contains conjugated double bonds, the absorption peak at a wavelength of 260 nm is the highest. By measuring the light absorption value of the sample at a wavelength of 260 nm, the concentration of the nucleic acid drug in the sample can be calculated.
[0035] The signal analysis unit 106 performs the above-mentioned data analysis and conversion functions as conventional software algorithm functions, which will not be elaborated in the embodiment of the present disclosure.
[0036] See again Figure 1 In an example of an embodiment of the present disclosure, the first liquid storage unit 101 includes a first liquid storage tank 111 and a first pipeline 112 , and the second liquid storage unit 102 includes a second liquid storage tank 121 and a second pipeline 122 .
[0037] The first liquid storage tank 111 and the second liquid storage tank 121 are arranged on the same horizontal plane, and the first pipe 112 connects the first liquid storage tank 111 and one of the two cavities 131 , and the second pipe 122 connects the second liquid storage tank 121 and the other of the two cavities 131 .
[0038] In this implementation, the first liquid storage unit 101 and the second liquid storage unit 102 adopt the above structure, and can be laid out in a left-right structure, which is simpler to implement.
[0039] In this implementation, the first pipe 112 and the second pipe 122 are transparent pipes, one of the two ultraviolet light sources 104 and one of the two ultraviolet light sensors 105 are correspondingly arranged on both sides of the first pipe 112, the other of the two ultraviolet light sources 104 and the other of the two ultraviolet light sensors 105 are correspondingly arranged on both sides of the second pipe 122, and the light emission directions of the two ultraviolet light sources 104 are parallel.
[0040] In this implementation, since the pipeline is closer to the biofilm, the sensitivity of the light intensity change caused by the buffer solution in the pipeline is better through ultraviolet light detection.
[0041] In an example of this implementation, the first pipe 112 and the second pipe 122 are U-shaped pipes.
[0042] The use of a U-shaped pipe can better achieve the connection between the liquid storage tank and the sample clamp cavity, and the U-shaped pipe can realize internal circulation, which is conducive to the detection of the transmembrane transport performance of drug molecules.
[0043] When a U-shaped pipe is used, the UV light source and the UV sensor may be arranged on both sides of the lowest point of the U-shaped pipe.
[0044] For example, taking the adjacent surfaces of the first liquid storage unit 101 and the second liquid storage unit 102 as the side surfaces, a pair of ultraviolet light sources and ultraviolet sensors are respectively disposed on the front and back surfaces of the first liquid storage unit 101 (or the second liquid storage unit 102 ).
[0045] In this implementation, the first liquid storage unit 101 has a first vent 113 , the second liquid storage unit 102 has a second vent 123 , the first pipe 112 is in communication with the first vent 113 , and the second pipe 122 is in communication with the second vent 123 .
[0046] The vent can be used to introduce mixed gas (usually 95% oxygen + 5% carbon dioxide) to maintain the tissue activity of the biofilm.
[0047] like Figure 1 The liquid storage tank and the pipeline are an integrated structure, for example, the tank and the pipeline are opened on the box body, and the first vent and the second vent are also opened on the box body, and the pipeline is connected by a pipeline. In this example, the entire box body can be transparent. The first liquid storage unit 101 and the second liquid storage unit 102 are symmetrically designed.
[0048] like Figure 1 As shown, the first liquid storage unit 101 and the second liquid storage unit 102 are arranged in close contact, and a sample clamp accommodating cavity A is provided at the joint, and the sample clamp 103 is inserted in the accommodating cavity A. The two cavities 131 of the sample clamp 103 are respectively connected to the connecting cavities B on both sides of the accommodating cavity A, and the two connecting cavities B are respectively connected to the first pipe 112 and the second pipe 122.
[0049] In the disclosed embodiment, the sample clamp 103 may be composed of two detachable parts, the two parts are hollowed out, and the two parts can clamp the biofilm, so that the cavity 131 is formed at the two hollowed out parts.
[0050] In the embodiment of the present disclosure, after the sample clamp 103 is inserted into the accommodating cavity A, a sealing structure is formed to prevent the buffer solution from overflowing.
[0051] In other examples of the embodiments of the present disclosure, the first liquid storage unit 101 may also include only the first liquid storage tank 111 , and the second liquid storage unit 102 may also include only the second liquid storage tank 121 .
[0052] The first liquid storage tank 111 is located above the second liquid storage tank 121 , and the bottom of the first liquid storage tank 111 is connected to one of the two cavities 131 , and the top of the second liquid storage tank 121 is connected to the other of the two cavities 131 .
[0053] In this implementation, the first liquid storage unit 101 and the second liquid storage unit 102 adopt the above structure and can be laid out in an upper and lower structure, which is more compact.
[0054] In this implementation, the first liquid storage tank 111 and the second liquid storage tank 121 are transparent liquid storage tanks, one of the two ultraviolet light sources 104 and one of the two ultraviolet light sensors 105 are correspondingly arranged on both sides of the first liquid storage tank 111, the other of the two ultraviolet light sources 104 and the other of the two ultraviolet light sensors 105 are correspondingly arranged on both sides of the second liquid storage tank 121, and the light emission directions of the two ultraviolet light sources 104 are parallel.
[0055] In this implementation, since there is no pipeline, the liquid storage tank can be directly tested.
[0056] See again Figure 1 The device may further include a shell 107 , in which the first liquid storage unit 101 , the second liquid storage unit 102 , the sample clamp 103 , the two ultraviolet light sources 104 , the two ultraviolet light sensors 105 and the signal analysis unit 106 are all located.
[0057] By setting up the shell, the internal brightness can be controlled, the detection of ultraviolet light can be facilitated, and the determination of permeability can be achieved.
[0058] See again Figures 2 to 4 The device may further include a display unit 108 , wherein the display unit 108 is located outside the housing 107 , and the display unit 108 is electrically connected to the signal analysis unit 106 .
[0059] The display unit is set to display the output of the signal analysis unit, thereby facilitating the experimenter to perform the experiment and record the experimental results.
[0060] See again Figure 2 and Figure 3 The display unit 108 can be embedded in the outer wall of the shell 107 to facilitate the arrangement of the display unit.
[0061] In the embodiment of the present disclosure, the display unit 108 may be a liquid crystal display or an organic light emitting diode display, etc.
[0062] In the embodiment of the present disclosure, the housing 107 , the first liquid storage unit 101 , the second liquid storage unit 102 , and the sample holder 103 may all be rectangular parallelepipeds.
[0063] In other embodiments, the above structure may also be in other shapes.
[0064] The above description is only an optional embodiment of the present disclosure and is not intended to limit the present disclosure. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present disclosure shall be included in the protection scope of the present disclosure.
Claims
1. A nucleic acid drug transmembrane transport analysis device, characterized in that: The device comprises: A first liquid storage unit (101) for storing a drug-containing buffer, a second liquid storage unit (102) for storing a blank buffer, a sample clamp (103) for clamping a biofilm, two ultraviolet light sources (104), two ultraviolet sensors (105) and a signal analysis unit (106); The sample holder (103) has two cavities (131), and the two cavities (131) are separated by the biofilm. The first liquid storage unit (101) is connected to one of the two cavities (131), and the second liquid storage unit (102) is connected to the other of the two cavities (131). One of the two ultraviolet light sources (104) and one of the two ultraviolet sensors (105) are correspondingly arranged on both sides of the first liquid storage unit (101), and the other of the two ultraviolet light sources (104) and the other of the two ultraviolet sensors (105) are correspondingly arranged on both sides of the second liquid storage unit (102). The signal analysis unit (106) is electrically connected to the two ultraviolet sensors (105) respectively.
2. The device according to claim 1, characterized in that The first liquid storage unit (101) comprises a first liquid storage tank (111) and a first pipeline (112), and the second liquid storage unit (102) comprises a second liquid storage tank (121) and a second pipeline (122); The first liquid storage tank (111) and the second liquid storage tank (121) are arranged on the same horizontal plane, and the first pipe (112) is connected to the first liquid storage tank (111) and one of the two cavities (131), and the second pipe (122) is connected to the second liquid storage tank (121) and the other of the two cavities (131).
3. The device according to claim 2, characterized in that The first pipe (112) and the second pipe (122) are transparent pipes, one of the two ultraviolet light sources (104) and one of the two ultraviolet light sensors (105) are correspondingly arranged on both sides of the first pipe (112), the other of the two ultraviolet light sources (104) and the other of the two ultraviolet light sensors (105) are correspondingly arranged on both sides of the second pipe (122), and the light emission directions of the two ultraviolet light sources (104) are parallel.
4. The device according to claim 1, characterized in that The first liquid storage unit (101) comprises a first liquid storage tank (111), and the second liquid storage unit (102) comprises a second liquid storage tank (121); The first liquid storage tank (111) is located above the second liquid storage tank (121), and the bottom of the first liquid storage tank (111) is connected to one of the two cavities (131), and the top of the second liquid storage tank (121) is connected to the other of the two cavities (131).
5. The device according to claim 2, characterized in that The first liquid storage tank (111) and the second liquid storage tank (121) are transparent liquid storage tanks; one of the two ultraviolet light sources (104) and one of the two ultraviolet light sensors (105) are correspondingly arranged on two sides of the first liquid storage tank (111); the other of the two ultraviolet light sources (104) and the other of the two ultraviolet light sensors (105) are correspondingly arranged on two sides of the second liquid storage tank (121); and the light emission directions of the two ultraviolet light sources (104) are parallel.
6. The device according to claim 2 or 3, characterized in that: The first pipe (112) and the second pipe (122) are U-shaped pipes.
7. The device according to claim 6, characterized in that The first liquid storage unit (101) has a first vent (113), the second liquid storage unit (102) has a second vent (123), the first pipe (112) is in communication with the first vent (113), and the second pipe (122) is in communication with the second vent (123).
8. The device according to any one of claims 1 to 5, characterized in that The device also includes a shell (107), and the first liquid storage unit (101), the second liquid storage unit (102), the sample holder (103), the two ultraviolet light sources (104), the two ultraviolet sensors (105) and the signal analysis unit (106) are all located in the shell (107).
9. The device according to claim 8, characterized in that The device further comprises a display unit (108), wherein the display unit (108) is located outside the housing (107), and the display unit (108) is electrically connected to the signal analysis unit (106).
10. The device according to claim 9, characterized in that The display unit (108) is embedded in the outer wall of the housing (107).