C-shaped deep breather pipe and stirring device

Abstract

The utility model relates to the technical field of cell culture, and aims to solve the technical problem that dissolved oxygen is insufficient or bubbles are broken to damage cells. In order to solve the technical problem, the utility model provides the C-shaped deep breather pipe and the stirring device. The device comprises an air guide pipe which extends upwards; an air inlet and an air outlet are respectively formed in the top end and the bottom end of the air guide pipe; the C-shaped ventilation pipe is of a C-shaped structure, a plurality of ventilation holes are formed in the C-shaped ventilation pipe, and the ventilation holes are formed in the bottom of the C-shaped ventilation pipe; wherein an air outlet of the air guide pipe is connected to the top of the middle position of the C-shaped breather pipe; in the circumferential direction of the C-shaped ventilation pipe, the circumference from the air guide pipe to one end of the C-shaped ventilation pipe is equal to the circumference from the air guide pipe to the other end of the C-shaped ventilation pipe; and the air guide pipe is communicated with the C-shaped breather pipe. According to the utility model, gas can be output more uniformly and stably; and mixed mass transfer and oxygen transfer are greatly provided.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a C-type deep ventilation tube and a stirring device. Background Art

[0002] In the prior art, deep aeration technology is used to optimize gas transmission, which can ensure that the microorganisms or cells in the bioreactor can obtain sufficient oxygen to maintain their metabolic activity and growth state. Deep aeration technology combines stirring and bubble dispersion methods to improve the efficiency of dissolved oxygen and the performance of biological reactions, but there are problems such as uneven bubble distribution and high shear stress, which lead to insufficient dissolved oxygen or bubble breakage and damage to cells. Utility Model Content

[0003] Therefore, the technical problem to be solved by the present invention is to overcome the above-mentioned problems existing in the prior art.

[0004] In order to solve the above technical problems, the utility model provides a C-type deep ventilation tube, comprising:

[0005] The air guide tube extends upward; the top and bottom ends of the air guide tube are respectively provided with an air inlet and an air outlet;

[0006] The C-type vent pipe is a C-shaped structure with a plurality of vent holes formed on the C-type vent pipe, and the vent holes are formed at the bottom of the C-type vent pipe;

[0007] Among them, the air outlet of the air duct is connected to the top of the middle position of the C-type ventilation tube; along the circumference of the C-type ventilation tube, the circumference from the air duct to one end of the C-type ventilation tube is equal to the circumference from the air duct to the other end of the C-type ventilation tube; the air duct is connected to the C-type ventilation tube.

[0008] In one embodiment of the utility model, along the circumference of the C-shaped ventilation pipe, the distance between two adjacent ventilation holes gradually decreases from the position where the air guide pipe is connected to the C-shaped ventilation pipe to the end of the C-shaped ventilation pipe.

[0009] In one embodiment of the present invention, the diameter of the vent hole is 0.2-0.3 mm.

[0010] In one embodiment of the utility model, an opening is provided at the end of the C-shaped ventilation pipe, and an end cap is detachably connected to the position where the opening is provided in the C-shaped ventilation pipe.

[0011] In one embodiment of the present invention, the end cap is threadedly connected to the C-type vent pipe.

[0012] In one embodiment of the present invention, the air duct includes a vertical section and a horizontal section connected to the bottom end of the vertical section, the air inlet is arranged at the top end of the vertical section, and the air outlet is arranged at one end of the horizontal section.

[0013] In one embodiment of the present invention, a circular arc transition is formed at the connection position between the vertical segment and the horizontal segment.

[0014] The utility model also provides a stirring device, comprising:

[0015] Tank;

[0016] The tank top plate is connected to the top of the tank body;

[0017] The stirring mechanism comprises a stirring shaft, a stirring driving member and a plurality of paddles, wherein the stirring shaft is connected to the stirring driving member, the stirring shaft is located in the tank body, and the stirring driving member is located on the top of the tank top plate; the plurality of paddles are arranged on the stirring shaft at intervals;

[0018] Such as the C-type deep ventilation tube in any of the above embodiments; the C-type ventilation tube in the C-type deep ventilation tube is located at the bottom of the tank body, the air guide tube in the C-type deep ventilation tube is in the tank body and extends upward through the tank top plate, and the top of the air guide tube is located above the tank top plate.

[0019] In one embodiment of the utility model, a through hole is provided on the tank top plate, a connector is connected in the through hole, and the air guide pipe is sealed and connected to the through hole via the connector.

[0020] In one embodiment of the utility model, the stirring drive member includes a motor disposed above the tank top plate, and the output shaft of the motor passes through the tank top plate and is connected to the top end of the stirring shaft.

[0021] The above technical solution of the utility model has the following advantages compared with the prior art:

[0022] The C-type deep ventilation tube and stirring device described in the utility model have an air guide tube connected to the middle position of the C-type ventilation tube, so that the distance from the air inlet position of the C-type ventilation tube to the end of the C-type ventilation tube is relatively short. In this way, under the same pressure, the bubble quantity and bubble size close to and far from the ventilation holes of the air guide tube are the same, that is, the gas is output more evenly and stably, the dissolved oxygen is sufficient, and the bubble breakage and cell damage are avoided.

[0023] In addition, the stirring shaft is driven to rotate by the stirring drive member, and the stirring shaft drives the blades to rotate clockwise. At the same time, external oxygen or compressed air is introduced into the C-type ventilation pipe located at the bottom of the tank body through the air guide tube. The multiple vents on the C-type ventilation pipe allow the oxygen or compressed air introduced into the C-type ventilation pipe to be evenly output. Driven by the stirring flow field of the blades, the bubbles diffuse to the surroundings and contact the cell culture fluid in the tank body, thereby effectively increasing the gas-liquid contact area and providing a large amount of mixed mass transfer and oxygen transfer. Secondly, the vent is opened at the bottom of the C-type ventilation pipe, so that under the drive of air pressure, the oxygen or compressed air first descends after coming out from the bottom, and then rises again under the action of buoyancy, thereby effectively increasing the residence time of the bubbles in the liquid, further increasing the oxygen transfer. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to make the content of the utility model easier to understand, the utility model is further described in detail according to the specific embodiments of the utility model in combination with the accompanying drawings, wherein:

[0025] Figure 1 This is a schematic diagram of the structure of a stirring device in a preferred embodiment of the utility model;

[0026] Figure 2 yes Figure 1 A schematic diagram of the structure inside a tank in a stirring device;

[0027] Figure 3 yes Figure 2 A top view of a stirring device;

[0028] Figure 4 yes Figure 3 A CC cross-sectional view of a stirring device;

[0029] Figure 5 yes Figure 1 A schematic diagram of the structure of a stirring mechanism and a C-type deep ventilation pipe in a stirring device;

[0030] Figure 6 yes Figure 1 Schematic diagram of the structure of a C-type deep ventilation tube in a stirring device Figure 1 ;

[0031] Figure 7 yes Figure 1 Schematic diagram of the structure of a C-type deep ventilation tube in a stirring device Figure 2 ;

[0032] Figure 8 yes Figure 1 A schematic diagram of the structure of a C-type ventilation pipe in a stirring device.

[0033] Description of the accompanying drawings in the specification: 100, tank body;

[0034] 200, tank top plate; 210, connector;

[0035] 300, stirring mechanism; 310, stirring shaft; 320, stirring drive member; 330, paddle;

[0036] 400, C-type deep ventilation tube; 410, air guide tube; 411, air inlet; 412, air outlet; 413, vertical section; 414, horizontal section; 420, C-type ventilation tube; 421, air hole; 422, end cap. DETAILED DESCRIPTION

[0037] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments so that those skilled in the art can better understand the present invention and implement it, but the embodiments are not intended to limit the present invention.

[0038] In some comparative embodiments, the C-type deep ventilation tube includes an airway tube and a semicircular airway tube, and a plurality of ventilation holes are provided on the outer side of the semicircular airway tube. The semicircular airway tube includes a proximal end and a distal end, and the airway tube is connected to the proximal end of the semicircular airway tube. In this way, the gas coming in from the airway tube is transported from the proximal end to the distal end. In this way, after ventilation, if the flow rate is low, bubbles are output from the air holes near the proximal end, thereby causing the pressure at the distal end to decrease, and no bubbles are output from the air holes near the distal end. If the flow rate is high, the ventilation pressure at the proximal end is too high, the bubbles are concentrated, and the bubble breaking shear force is too large, which damages the cells.

[0039] Reference Figures 1 to 8 As shown, in order to solve the problems of the above-mentioned comparative embodiment, the embodiment of the utility model provides a stirring device, including: a tank body 100, a tank top plate 200, a stirring mechanism 300 and a C-type deep ventilation pipe 400.

[0040] The tank top plate 200 is connected to the top of the tank body 100;

[0041] The stirring mechanism 300 includes a stirring shaft 310, a stirring driving member 320 and a plurality of paddles 330. The stirring shaft 310 is connected to the stirring driving member 320. The stirring shaft 310 is located in the tank body 100. The stirring driving member 320 is located on the top of the tank top plate 200. The plurality of paddles 330 are arranged at intervals on the stirring shaft 310.

[0042] The C-type deep ventilation tube 400 includes an airway tube 410 and a C-type ventilation tube 420. The airway tube 410 extends upward, and the top and bottom ends of the airway tube 410 are respectively provided with an air inlet 411 and an air outlet 412; the C-type ventilation tube 420 is a C-type structure, and a plurality of ventilation holes 421 are opened on the C-type ventilation tube 420, for example, 6 ventilation holes 421 are opened. The ventilation holes 421 are opened at the bottom of the C-type ventilation tube 420; the air outlet 412 of the airway tube 410 is connected to the top of the middle position of the C-type ventilation tube 420; along the circumference of the C-type ventilation tube 420, the circumference from the airway tube 410 to one end of the C-type ventilation tube 420 is equal to the circumference from the airway tube 410 to the other end of the C-type ventilation tube 420; the airway tube 410 is connected to the C-type ventilation tube 420. A plurality of vent holes 421 are symmetrically arranged on the C-shaped vent pipe 420 along the position where the air guide pipe 410 is connected to the C-shaped vent pipe 420 .

[0043] Among them, the C-type ventilation pipe 420 in the C-type deep ventilation pipe 400 is located at the bottom of the tank body 100, the air guide pipe 410 in the C-type deep ventilation pipe 400 is in the tank body 100 and extends upward through the tank top plate 200, and the top of the air guide pipe 410 is located above the tank top plate 200.

[0044] The external air tube is connected to the airway tube 410, and the external air tube is opened to ventilate the C-type deep ventilation tube 400, and the air flows along the airway tube 410 to the center of the C-type ventilation tube 420, and then evenly disperses to both ends of the C-type ventilation tube 420. The C-type ventilation tube 420 is symmetrically provided with ventilation holes 421 of the same aperture on both sides of the airway tube 410.

[0045] Specifically, the airway tube 410 of the present application is connected to the middle position of the C-type ventilation tube 420, so that the distance from the air inlet position of the C-type ventilation tube 420 to the end of the C-type ventilation tube 420 is relatively short. In this way, under the same pressure, the bubble quantity and bubble size close to and far from the ventilation hole 421 of the airway tube 410 are the same, that is, the gas is output more evenly and stably, the dissolved oxygen is sufficient, and the bubble breakage and cell damage are avoided.

[0046] In addition, the stirring shaft 310 is driven to rotate by the stirring driving member 320, and the stirring shaft 310 drives the blade 330 to rotate clockwise. At the same time, the external oxygen or compressed air is introduced into the C-type vent pipe 420 located at the bottom of the tank body 100 through the air guide pipe 410. The multiple vents 421 on the C-type vent pipe 420 allow the oxygen or compressed air introduced into the C-type vent pipe 420 to be uniformly output. Driven by the stirring flow field of the blade 330, the bubbles diffuse to the surroundings and contact the cell culture liquid in the tank body 100, thereby effectively increasing the gas-liquid contact area and providing mixed mass transfer and oxygen transfer to a large extent. Secondly, the vents 421 are opened at the bottom of the C-type vent pipe 420, so that under the drive of air pressure, the oxygen or compressed air first descends after coming out from the bottom, and then rises again under the action of buoyancy, thereby effectively increasing the residence time of the bubbles in the liquid, further increasing the oxygen transfer.

[0047] Furthermore, along the circumference of the C-type vent pipe 420, the distance between two adjacent vent holes 421 gradually decreases from the position where the air guide pipe 410 and the C-type vent pipe 420 are connected to the end of the C-type vent pipe 420. That is, the vent holes 421 near the position where the air guide pipe 410 and the C-type vent pipe 420 are connected are sparsely arranged, and the vent holes 421 far from the position where the air guide pipe 410 and the C-type vent pipe 420 are connected are densely arranged. Specifically, this embodiment makes the vent holes 421 at the air inlet position of the C-type vent pipe 420 and the end position thereof output the same amount of bubbles, so that a more uniform and stable output can be achieved.

[0048] Furthermore, the diameter of the vent 421 is 0.2-0.3mm. Taking full account of the dissolved oxygen and mass transfer and production requirements, if the aperture of the vent 421 is too large, the bubbles will be too large. Under the stirring and cutting of the stirring blade, the cells will be easily broken, and the shear force will be too large to easily damage the cells; if the aperture of the vent 421 is too small, under the stirring of the stirring blade, the cells will be less broken and the bubbles will accumulate more in the tank body, which will lead to more foam in the liquid, and finally may cause the foam to overflow the tank body. After experimental verification, when the size of the opening of the vent 421 is 0.2-0.3mm, the mass transfer effect in the cell culture process is good and the cell damage is small.

[0049] In some comparative embodiments, the end of the C-type vent 420 is closed. Because during long-term use, the vent 421 is easily blocked by cells due to its relatively small diameter. In this way, if it is not easy to clean after being blocked, it can only be remade and replaced if it is not cleaned in place, which affects the planned progress of cell culture and also increases the cost. Therefore, in response to this problem, this embodiment further provides an opening at the end of the C-type vent 420, and the position where the opening is provided in the C-type vent 420 is detachably connected with an end cap 422, and the opening is connected to the vent 421. In some embodiments, the end cap 422 is threadedly connected to the C-type vent 420. It can be seen that the detachable design of this embodiment enables maintenance personnel to easily separate the C-type vent 420 and the end cap 422, and conduct a thorough internal cleaning, which helps to remove accumulated dirt, sediment and microorganisms and maintain good sanitary conditions. In addition, timely removal of corrosive substances and sediments can reduce corrosion and wear on the C-type vent 420 and extend the service life of the C-type vent 420.

[0050] Specifically, in this embodiment, the end cap 422 is removed after blocking, which is convenient for cleaning and unblocking of the C-type vent pipe 420 and the vent hole 421 after later use, and the end cap 422 can be installed at the opening when in use.

[0051] Further, the air guide pipe 410 includes a vertical section 413 and a horizontal section 414 connected to the bottom end of the vertical section 413, an air inlet 411 is arranged at the top end of the vertical section 413, and an air outlet 412 is arranged at one end of the horizontal section 414. A circular arc transition is formed at the connection position between the vertical section 413 and the horizontal section 414. Specifically, the present embodiment can stably and reliably deliver gas to the C-shaped vent pipe 420.

[0052] Furthermore, a through hole is provided on the tank top plate 200 , a connector 210 is connected in the through hole, and the air guide pipe 410 is sealedly connected to the through hole via the connector 210 .

[0053] Furthermore, the stirring driving member 320 includes a motor disposed above the tank top plate 200, and the output shaft of the motor passes through the tank top plate 200 and is connected to the top end of the stirring shaft 310. Specifically, the structure of this embodiment is stable and reliable.

[0054] The present application can achieve uniform mass transfer in the tank body 100 through stirring by the upper and lower double-layer paddles 330; and combined with the design of the C-type ventilation pipe 420 with the vent hole 421, it can achieve fast oxygen transfer and sufficient oxygen supply in the bioreactor (tank body 100); when the paddles 330 and the C-type ventilation pipe 420 work at the same time, the gas output is more uniform, and the shear force on the cells in the bioreactor is greatly reduced, so that the cell survival rate is higher.

[0055] Obviously, the above embodiments are merely examples for the purpose of clear explanation and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention of the utility model.

Claims

1. A C-type deep ventilation tube, characterized in that: include: An air guide pipe extends upward; the top and bottom ends of the air guide pipe are respectively provided with an air inlet and an air outlet; The C-type ventilation pipe is a C-type structure, and a plurality of ventilation holes are provided on the C-type ventilation pipe, and the ventilation holes are provided at the bottom of the C-type ventilation pipe; Among them, the air outlet of the air duct is connected to the top of the middle position of the C-type ventilation pipe; along the circumference of the C-type ventilation pipe, the circumference from the air duct to one end of the C-type ventilation pipe is equal to the circumference from the air duct to the other end of the C-type ventilation pipe; the air duct is connected to the C-type ventilation pipe.

2. The C-type deep ventilation tube according to claim 1, characterized in that: Along the circumference of the C-shaped ventilation pipe, from the position where the air guide pipe is connected to the C-shaped ventilation pipe to the end of the C-shaped ventilation pipe, the distance between two adjacent ventilation holes gradually decreases.

3. The C-type deep ventilation tube according to claim 1, characterized in that: The diameter of the vent hole is 0.2-0.3 mm.

4. The C-type deep ventilation tube according to claim 1, characterized in that: An opening is provided at the end of the C-shaped ventilation pipe, and an end cap is detachably connected to the position where the opening is provided in the C-shaped ventilation pipe.

5. The C-type deep ventilation tube according to claim 4, characterized in that: The end cap is threadedly connected to the C-type vent pipe.

6. The C-type deep ventilation tube according to claim 1, characterized in that: The air duct includes a vertical section and a horizontal section connected to the bottom end of the vertical section, the air inlet is arranged at the top end of the vertical section, and the air outlet is arranged at one end of the horizontal section.

7. The C-type deep ventilation tube according to claim 6, characterized in that: A circular arc transition is formed at a connection position between the vertical section and the horizontal section.

8. A stirring device, characterized in that: include: Tank; A tank top plate connected to the top of the tank body; A stirring mechanism, comprising a stirring shaft, a stirring driving member and a plurality of paddles, wherein the stirring shaft is connected to the stirring driving member, the stirring shaft is located in the tank body, and the stirring driving member is located on the top of the tank top plate; the plurality of paddles are arranged on the stirring shaft at intervals; A C-type deep ventilation tube as described in any one of claims 1 to 7; the C-type ventilation tube in the C-type deep ventilation tube is located at the bottom of the tank body, the air guide tube in the C-type deep ventilation tube is in the tank body and extends upward through the tank top plate, and the top of the air guide tube is located above the tank top plate.

9. The stirring device according to claim 8, characterized in that: The tank top plate is provided with a through hole, the through hole is connected with a connector, and the air guide pipe is sealed and connected to the through hole via the connector.

10. The stirring device according to claim 9, characterized in that: The stirring driving member comprises a motor arranged above the tank top plate, and the output shaft of the motor passes through the tank top plate and is connected with the top end of the stirring shaft.

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