Bacterial liquid counting and diluting device and application thereof

Abstract

The utility model provides a bacteria liquid counting and diluting device which comprises a shell, a gantry cross beam assembly, a sampling system, a detection system, a reagent tube head and ultraviolet lamp system, a reagent tube and reagent tube head withdrawing system, a liquid path system and a circuit control system, the sampling system, the detection system, the reagent tube head and ultraviolet lamp system, the reagent tube and reagent tube head withdrawing system, the liquid path system and the circuit control system are all installed on the gantry beam assembly, and the reagent tube and reagent tube head withdrawing system comprises a target tube and an original tube. The detection system detects the number of bacteria in the bacterial liquid in the original tube, the concentration of the bacterial liquid in the original tube is obtained according to the number of bacteria, and the required volume of the bacterial liquid in the original tube is sucked and added into the target tube. According to the utility model, the number of bacteria in a sample bacteria liquid can be rapidly and accurately counted, and the concentration is calculated, so that the bacteria liquid is diluted.

Description

Technical Field

[0001] The utility model relates to the field of biomedical medical devices, and more specifically, to a new bacterial liquid counting and dilution device and application thereof. Background Art

[0002] Cell counters mostly refer to instruments that measure the number of platelets, white blood cells, red blood cells, etc. Fully automatic cell counters are widely used. The Coulter principle analysis method has always been an internationally recognized standard control method for measuring cell and particle size, and has always occupied an important position in hematological analysis.

[0003] In addition, the bacterial turbidity determination currently mainly used in biological products is determined by turbidimetry. Although this method is simple and fast, it can only detect suspensions containing a large number of bacteria and obtain relative bacterial numbers. It cannot be used for samples that are too dark in color. Therefore, turbidimetry is only a method for roughly determining bacterial density, and the accuracy of the determination is not high.

[0004] Therefore, how to design an automated bacterial liquid sample dilution device that can accurately, quickly and conveniently target bacteria is a technical problem to be solved by the present utility model. Utility Model Content

[0005] The utility model provides a bacterial liquid counting and diluting device, which at least solves the technical problems of low measurement accuracy and long dilution time in the bacterial liquid dilution process.

[0006] The utility model provides a bacterial liquid counting and dilution device, which comprises a shell, a gantry beam assembly, a sampling system, a detection system, a reagent tube head and an ultraviolet lamp system, a reagent tube and a reagent return tube head system, a liquid circuit system and a circuit control system. The sampling system, the detection system, the reagent tube head and the ultraviolet lamp system, the reagent tube and the reagent return tube head system, the liquid circuit system and the circuit control system are all installed on the gantry beam assembly, the shell is fixed to the bottom of the gantry beam assembly, the reagent tube and the reagent return tube head system comprises a target tube and an original tube, the detection system detects the number of bacteria in the bacterial liquid in the original tube, obtains the concentration of the bacterial liquid in the original tube according to the number of bacteria, and absorbs the required volume of the bacterial liquid in the original tube and adds it to the target tube.

[0007] Optionally, the concentration of the bacterial solution in the original tube is obtained according to the number of bacteria, and the required volume of the bacterial solution and the diluent in the original tube are drawn and added to the target tube.

[0008] Optionally, the sampling system includes a sampling cantilever and a reagent tube head adapter, and the reagent tube head adapter is fixed to the sampling cantilever.

[0009] Optionally, the detection system includes a jewel hole, a front pool, a rear pool and electrodes. The front pool and the rear pool are connected through the jewel hole. There is one electrode on each side of the jewel hole. The liquid pressure between the front pool and the rear pool is negative pressure.

[0010] Optionally, the negative pressure is used to allow the bacterial liquid to be counted to pass from the front pool through the gemstone hole into the rear pool.

[0011] Optionally, the reagent tube head and ultraviolet lamp system include a reagent tube head and an ultraviolet lamp system, the ultraviolet lamp system is located above the reagent tube head, and the ultraviolet lamp system is used to sterilize the reagent tube head.

[0012] Optionally, the number of the above reagent tube heads is greater than one.

[0013] Optionally, the sampling system is moved above the reagent tube head and the reagent tube head is fixed to the reagent tube head adapter.

[0014] Optionally, the reagent tube and reagent tube head return system removes the used reagent tube heads.

[0015] The above-mentioned bacterial liquid counting and dilution device is used in diluting the target bacterial liquid, and the above-mentioned application includes:

[0016] Next steps:

[0017] The first step is to add the prepared bacterial solution into the original tube and place it into the fixed position of the original tube in the bacterial solution counting and dilution device;

[0018] Step 2: placing the target tube into the fixed position of the target tube in the bacterial liquid counting and dilution device;

[0019] Step 3: Load the reagent tube head through the reagent tube head and the ultraviolet lamp system, absorb part of the bacterial solution in the original tube, add it to the detection system for bacterial counting, and the host computer calculates the concentration of the bacterial solution through the software algorithm;

[0020] Step 4: The bacterial solution counting and dilution device further calculates the volume of bacterial solution to be added based on the concentration of the bacterial solution calculated by the host computer software and the required concentration of the target bacterial solution;

[0021] Step 5: The used reagent tube head is returned through the reagent tube and reagent tube head return system, and the required volume of the bacterial solution to be added to the target tube is drawn from the original tube according to the calculated volume of the bacterial solution to be added to the target tube, and then added to the target tube;

[0022] Step 6: Take out the target tube from the bacterial solution counting and dilution device, and the counting and dilution is completed.

[0023] The utility model provides an automatic bacterial liquid sample pre-treatment dilution device, namely a bacterial liquid counting and dilution device. First, the number of bacteria needs to be measured quickly and accurately, and then the upper computer software calculates the concentration of the bacterial liquid sample through the obtained number of bacteria, and then the bacterial liquid sample is automatically diluted to obtain the target concentration bacterial liquid we need.

[0024] The technical solution of the utility model can quickly and accurately count the number of bacteria in the sample bacterial solution and calculate the concentration. On the basis of obtaining the sample bacterial solution concentration, the upper computer calculates the sample bacterial solution volume required for the target bacterial solution, and the lower computer drives the plunger pump to accurately and automatically prepare the required diluted bacterial solution in the main dilution cup. Compared with the turbidity meter currently used in the market for measuring bacterial solution concentration, the measurement accuracy of the utility model is improved by 3 orders of magnitude, so the accuracy of the dilution ratio of the bacterial solution concentration is also greatly improved. For hospitals and research institutions that need to accurately configure the target bacterial solution, the target bacterial solution concentration obtained by this instrument will be more real and accurate. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] By reading the detailed description below with reference to the accompanying drawings, the above and other objects, features and advantages of the exemplary embodiments of the present invention will become readily understood. In the accompanying drawings, several embodiments of the present invention are shown in an exemplary and non-limiting manner, wherein:

[0026] Figure 1 This is a schematic diagram of an optional bacterial liquid counting and dilution device according to an embodiment of the utility model;

[0027] Figure 2 This is a schematic diagram of the outer shell of an optional bacterial liquid counting and dilution device according to an embodiment of the utility model;

[0028] Figure 3 This is a schematic diagram of the internal structure of an optional bacterial liquid counting and dilution device according to an embodiment of the utility model;

[0029] Figure 4 This is a schematic diagram of a sampling system of an optional bacterial liquid counting and dilution device according to an embodiment of the utility model;

[0030] Figure 5 This is a schematic diagram of a liquid circuit system of an optional bacterial liquid counting and dilution device according to an embodiment of the utility model;

[0031] Figure 6 This is a schematic diagram of a detection system of an optional bacterial liquid counting and dilution device according to an embodiment of the utility model;

[0032] Figure 7This is a schematic diagram of a circuit control system of an optional bacterial liquid counting and dilution device according to an embodiment of the utility model;

[0033] Figure 8 A schematic diagram of a Tip head (pipette head) and an ultraviolet lamp system of an optional bacterial liquid counting and dilution device according to an embodiment of the utility model;

[0034] Fig. 9 This is a schematic diagram of a reagent tube and a Tip head system of an optional bacterial liquid counting and dilution device according to an embodiment of the utility model;

[0035] Fig.10 It is a schematic diagram of a gantry beam assembly of an optional bacterial liquid counting and dilution device according to an embodiment of the utility model;

[0036] Fig.11 An optional bacterial liquid counting and dilution device according to an embodiment of the utility model Figure 4 , Figure 6 , Figure 8 , Fig. 9 as well as Fig.10 Assembly diagram of

[0037] Fig.12 An optional bacterial liquid counting and dilution device according to an embodiment of the utility model Fig.11 , Figure 5 as well as Figure 7 Assembly diagram of .

[0038] The following specific implementation is used to further illustrate but not limit the present invention. The following embodiment is only a preferred implementation of the present invention. DETAILED DESCRIPTION

[0039] The principle and spirit of the present invention will be described below with reference to several exemplary embodiments. It should be understood that these embodiments are provided only to enable those skilled in the art to better understand and implement the present invention, and are not intended to limit the scope of the present invention in any way. On the contrary, these embodiments are provided to make the disclosure of the present application more thorough and complete, and to fully convey the scope of the disclosure of the present application to those skilled in the art.

[0040] The utility model is implemented as follows Figures 1 to 12 shown.

[0041] The present invention provides an automatic bacterial liquid sample pre-treatment dilution device, namely a bacterial liquid counting and dilution device. First, the number of bacteria needs to be measured quickly and accurately, and then the host computer software calculates the concentration of the bacterial liquid sample by the obtained number of bacteria, and then the bacterial liquid sample is automatically diluted to obtain the required target concentration bacterial liquid.

[0042] The Tip head mentioned in this embodiment and the accompanying drawings is the reagent tube head mentioned in the claims and the specification, which is a common feature in the industry.

[0043] Specifically, if Figure 1 , Figure 3 , Fig.11 and Fig.12 As shown, the bacterial liquid counting and dilution device provided by the embodiment of the utility model comprises a housing 1, a gantry beam assembly 2, a sampling system 3, a detection system 4, a reagent tube head and ultraviolet lamp system 5, a reagent tube and a reagent return tube head system 6, a liquid circuit system 7 and a circuit control system 8. The sampling system 3, the detection system 4, the reagent tube head and ultraviolet lamp system 5, the reagent tube and the reagent return tube head system 6 are all installed on the gantry beam assembly 2, the liquid circuit system 7, and the circuit control system 8 are also installed on the gantry beam assembly 2, and the outermost layer is covered with the housing 1, and the housing 1 is fixed to the bottom of the gantry beam assembly 2 with bolts.

[0044] The housing 1 is mainly made of sheet metal, which has good shielding effect and good heat dissipation.

[0045] like Figure 4 The movement position of the above-mentioned sampling system 3 is shown as follows Figure 3 As shown, the sampling system 3 is used to obtain bacterial samples to be counted and bacterial liquid samples for dilution, and the sampling system 3 includes a sampling motor plate 31, a sampling motor 32, a sampling guide rail 33, a sampling slider 34, a sampling rack 35, a sampling gear 36, a sampling pump plate 37, a sampling pump 38, a sampling cantilever 39 and a reagent tube head adapter 310. The sampling slider 34 of the sampling system 3 is fixed on the sampling motor plate 31, and the sampling guide rail 33, the sampling rack 35, the sampling pump 38 and the sampling cantilever 39 are fixed on the sampling pump plate 37. The sampling gear 36 is fixed on the sampling motor 32, and the sampling motor 32 is fixed to the sampling motor plate 31. The reagent tube head 51 is fixed to the reagent tube head adapter 310 and then fixed to the sampling cantilever 39.

[0046] The improvement of the above sampling system 3 is that the movement is faster and the tip piercing force is stronger.

[0047] like Figure 5The liquid circuit system 7 shown is used for adding diluent and flushing the front and rear pools. The liquid circuit system 7 includes a counting pool 701, a diluent barrel 702, a waste liquid barrel 703, a plunger pump 704, a vacuum pool 705, an air pressure sensor 706, a first diaphragm pump 707, a second diaphragm pump 708, a first three-way valve 709, a second three-way valve 710, a first two-way valve 711, a second two-way valve 712, a third two-way valve 713, an air filter 714, a third three-way valve 715 and an alcohol barrel 716. The above-mentioned diluent barrel 702 is connected to the normally open end of the above-mentioned third three-way valve 715, the above-mentioned alcohol barrel 716 is connected to the normally closed end of the above-mentioned third three-way valve 715, the common end of the above-mentioned third three-way valve 715 is connected to the normally open end of the above-mentioned first three-way valve 709, the common end of the above-mentioned first three-way valve 709 is connected to the above-mentioned plunger pump 704, the normally closed end of the above-mentioned first three-way valve 709 is connected to the common end of the above-mentioned second three-way valve 710, and the normally closed end of the above-mentioned second three-way valve 710 is connected to the interface at the top of the counting pool 701.

[0048] like Figure 6 The detection system 4 shown includes a jewel hole 41, a front pool 42, a rear pool 43 and an electrode 44, wherein the front pool 42 and the rear pool 43 are connected through the jewel hole 41, and there is one electrode 44 on each side of the jewel hole 41. The liquid pressure between the front pool 42 and the rear pool 43 is a negative pressure, and the negative pressure is used to make the bacterial sample to be counted enter the rear pool 43 from the front pool 42 through the jewel hole 41, so as to count the bacteria in the bacterial sample to be counted.

[0049] like Figure 7 The circuit control system 8 shown is used to determine the number of bacteria in the above-mentioned bacterial sample to be counted according to the pulse signal generated on both sides of the above-mentioned gem hole 41 when the pulse signal is detected, wherein the above-mentioned pulse signal is used to indicate that the bacteria in the above-mentioned bacterial sample to be counted have passed through the above-mentioned gem hole. In addition, the control of various motors, valves, and pumps is realized by this system.

[0050] System analysis software: The upper computer software calculates the concentration of the bacterial sample, and then calculates the volume of the bacterial solution sample that needs to be diluted, and performs automatic dilution to obtain the required target concentration of bacterial solution.

[0051] like Figure 8 The reagent tube head and ultraviolet lamp system 5 of the bacterial liquid counting and diluting instrument shown in the figure include a reagent tube head 51 and an ultraviolet lamp system 52. The reagent tube head 51 is used to absorb the sample bacterial liquid. The reagent tube head 51 is a disposable consumable. The ultraviolet lamp system 52 is used to sterilize the reagent tube head 51 to ensure that the reagent tube head 51 is sterile. The embodiment of the utility model adopts the following method: Figure 8The 96-well reagent tube head and ultraviolet lamp system 5 shown, specifically, can accommodate ninety-six of the above reagent tube heads 51, which greatly improves the detection efficiency.

[0052] like Figure 8 The reagent tube head and ultraviolet lamp system 5 of the bacterial liquid counting and diluting instrument shown include a reagent tube head 51, an ultraviolet lamp system 52, a pipetting head support plate 53, a pipetting head guide rail 54, a pipetting head slider 55, and a pipetting head motor 56. The ultraviolet lamp system 52 is installed on the inner side of the reagent tube head 51 and fixed together with the pipetting head guide rail 54. The pipetting head slider 55 and the pipetting head motor 56 are fixed to the pipetting head support plate 53. The reagent tube head 51 is used to absorb the sample bacterial liquid. The reagent tube head 51 is a disposable consumable. The ultraviolet lamp system 52 is used to sterilize the reagent tube head 51 to ensure that the reagent tube head 51 is sterile.

[0053] like Fig. 9 The reagent tube and reagent return tube head system 6 of the bacterial liquid counting and dilution device shown in the figure includes a reagent tube and a reagent return tube head system 62. The reagent tube is used to hold the original bacterial liquid of the sample bacterial liquid and the target concentration bacterial liquid, such as Fig. 9 The left side is a target tube 611 for holding bacterial solution of target concentration, and the right side is an original tube 612 for holding original bacterial solution of sample bacterial solution; the above-mentioned reagent tube head return system 62 is used to remove the used reagent tube head to facilitate the use of new reagent tube head.

[0054] like Fig.10 The gantry beam assembly 2 of the bacterial liquid counting and dilution device shown is used to move the sampling system 3, and the gantry beam assembly 2 includes a gantry frame 21, a translation guide rail 22, a translation slider 23, a translation motor 24, a translation transmission belt 25, and a translation guide wheel 26. The translation guide rail 22, the translation motor 24, and the translation guide wheel 26 are fixed to the upper part of the gantry frame 21, the translation slider 23 is on the translation guide rail 22, one end of the translation transmission belt 25 is sleeved on the output shaft of the translation motor 24, and the other end is sleeved on the translation guide wheel 26, and is fixed to the translation slider 23 in the middle.

[0055] like Fig.11 , 12 The schematic diagram of the assembly of the bacterial liquid counting and dilution device is shown. Fig.11 An optional bacterial liquid counting and dilution device according to an embodiment of the utility model Figure 4 , Figure 6 , Figure 8 , Fig. 9 as well as Fig.10 Assembly diagram of Fig.12 An optional bacterial liquid counting and dilution device according to an embodiment of the utility model Fig.11 , Figure 5 as well as Figure 7 Assembly diagram of .

[0056] The operation process of the above bacterial liquid counting and dilution device is as follows:

[0057] The first step is to add the prepared bacterial solution sample into the initial tube and place it in the initial tube position of the instrument;

[0058] Step 2: Place the target tube in the target tube position of the instrument;

[0059] The third step: Use the original bacterial solution pump to load the reagent tube head, suck part of the bacterial solution sample from the initial tube, add it to the counting pool for bacterial counting, and the upper computer calculates the concentration of the bacterial solution sample, that is, the initial tube, through the software algorithm;

[0060] Step 4: The instrument further calculates the volume of bacterial solution sample that needs to be added to the target tube based on the concentration of the initial tube calculated by the host computer software;

[0061] Step 5: The bacterial solution sample pump unloads the used reagent tube head and loads a new reagent tube head. According to the calculated bacterial solution sample volume to be added to the target tube, the required bacterial solution sample is drawn from the initial tube and then added to the target tube.

[0062] Step 6: Remove the target tube from the instrument, screw on the cap, and the counting dilution process is complete.

[0063] The advantage of the embodiment of the utility model is that the number of bacteria in the sample bacterial solution can be quickly and accurately counted and the concentration can be calculated. Based on the sample bacterial solution concentration, the upper computer calculates the sample bacterial solution volume required for the target tube, and the lower computer drives the plunger pump to accurately and automatically prepare the required diluted bacterial solution in the target tube. Compared with the turbidity meter currently used in the market for measuring bacterial solution concentration, the measurement accuracy of the embodiment of the utility model is improved by 3 orders of magnitude, so the accuracy of the diluted bacterial solution concentration is also greatly improved. For hospitals and research institutions that need to accurately configure the target bacterial solution, the target bacterial solution concentration obtained by this instrument will be more real and accurate.

Claims

1. A bacterial liquid counting and dilution device, characterized in that: The bacterial liquid counting and dilution device comprises a housing (1), a gantry beam assembly (2), a sampling system (3), a detection system (4), a reagent tube head and ultraviolet lamp system, a reagent tube and reagent return tube head system (6), a liquid circuit system (7) and a circuit control system (8). The sampling system (3), the detection system (4), the reagent tube head and ultraviolet lamp system, the reagent tube and reagent return tube head system (6), the liquid circuit system (7) and the circuit control system (8) are all installed on the gantry beam assembly (2). The housing (1) is fixed to the bottom of the gantry beam assembly (2). The reagent tube and reagent return tube head system (6) comprises a target tube (611) and an original tube (612). The detection system (4) detects the number of bacteria in the bacterial liquid in the original tube (612), obtains the concentration of the bacterial liquid in the original tube (612) according to the number of bacteria, and draws the required volume of the bacterial liquid in the original tube and adds it to the target tube (611).

2. The bacterial liquid counting and dilution device according to claim 1, characterized in that: The concentration of the bacterial solution in the original tube (612) is obtained based on the number of bacteria, and the required volume of the bacterial solution and the diluent in the original tube are drawn and added to the target tube (611).

3. The bacterial liquid counting and dilution device according to claim 1, characterized in that: The sampling system (3) comprises a sampling cantilever (39) and a reagent tube head adapter (310), wherein the reagent tube head adapter (310) is fixed to the sampling cantilever (39).

4. The bacterial liquid counting and dilution device according to claim 1, characterized in that: The detection system (4) comprises a jewel hole (41), a front pool (42), a rear pool (43) and an electrode (44); the front pool (42) and the rear pool (43) are connected via the jewel hole (41); there is one electrode (44) on each side of the jewel hole (41); and the liquid pressure between the front pool (42) and the rear pool (43) is negative pressure.

5. The bacterial liquid counting and dilution device according to claim 4, characterized in that: The negative pressure is used to allow the bacterial liquid to be counted to enter the rear pool (43) from the front pool (42) through the jewel hole (41).

6. The bacterial liquid counting and dilution device according to claim 3, characterized in that: The reagent tube head and ultraviolet lamp system comprises a reagent tube head (51) and an ultraviolet lamp system (52), wherein the ultraviolet lamp system (52) is located above the reagent tube head (51), and the ultraviolet lamp system (52) is used to sterilize the reagent tube head (51).

7. The bacterial liquid counting and dilution device according to claim 6, characterized in that: The number of the reagent tube heads (51) is greater than one.

8. The bacterial liquid counting and dilution device according to claim 6, characterized in that: The sampling system (3) is moved above the reagent tube head (51) and the reagent tube head (51) is fixed to the reagent tube head adapter (310).

9. The bacterial liquid counting and dilution device according to claim 8, characterized in that: The reagent tube and reagent tube head removal system (6) removes the used reagent tube head (51).

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