Cell culture dish for focused shock wave cell treatment
By designing a rounded bench-shaped cell culture chamber and an anti-slip coated cell culture dish, the problems of dispersion, splashing and uneven processing of traditional cell culture dishes in focus shock wave processing are solved, and efficient utilization of focus shock waves and the accuracy of cell processing are achieved.
Patent Information
- Application Number
- CN202421517842.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-30
- Publication Date
- 2025-06-06
- Estimated Expiration
- 2034-06-30
AI Technical Summary
In the focus shock wave treatment experiments, traditional cell culture dishes have problems such as dispersion of cell transplantation positions, splashing of cell fluid caused by shock wave vibration, and uneven shock wave treatment.
A cell culture dish for focusing shock wave treatment was designed, using a rounded table-shaped cell culture cavity and anti-slip coating, equipped with a removable dish lid and high-pressure resistant material, ensuring shock wave energy concentration, cell stability and safety.
The concentrated utilization of focused shock wave energy is achieved, ensuring the accuracy and consistency of cell processing, while improving the stability and safety of the Petri dish in a high-pressure environment.
Smart Images
Figure CN222948367U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell culture, in particular to a cell culture dish used for focused shock wave treatment. Background Art
[0002] In cell culture and experiments, using focused shock waves to treat cells has become an important experimental method. When propagating, shock waves can release energy at a speed that exceeds the speed of sound in the medium. They can not only penetrate liquids and solids, but also directly act on the cell surface. In this process, it may change the structure of the cell, thereby causing corresponding physiological reactions. Therefore, focused shock waves can act on cells accurately to achieve changes and regulation of cell states.
[0003] Existing cell culture dishes usually consist of a flat disc-shaped dish bottom and a dish cover. The dish bottom is used to culture biological cells. However, there are some problems with traditional cell culture dishes in focused shock wave treatment experiments. For example, the cell planting positions are scattered, and the focused shock waves cannot be received in a centralized manner. The splashing of cell fluid caused by shock wave vibration will also cause uneven shock wave treatment. Therefore, traditional cell culture dishes are often not directly used for focused shock wave treatment of cells.
[0004] Therefore, it is necessary to develop a new type of cell culture dish to meet the experimental requirements of focused shock wave treatment. Utility Model Content
[0005] The utility model aims to provide a cell culture dish for focusing shock wave treatment of cells in order to overcome the defect that the traditional cell culture dish is not suitable for focusing shock wave treatment experiment.
[0006] The purpose of the utility model can be achieved through the following technical solutions:
[0007] A cell culture dish for treating cells with focused shock waves, comprising a cylindrical culture dish body;
[0008] The culture dish body is provided with a truncated cone-shaped cell culture cavity, which is a structure with a wide top and a narrow bottom, and the bottom of the cell culture cavity is a horizontal cell planting area.
[0009] Furthermore, the cell planting area and the top of the cell culture chamber are arranged in a concentric circle.
[0010] Furthermore, the cell planting area is located in the center of the culture dish body.
[0011] Furthermore, the diameter ratio of the cell planting area to the top of the cell culture chamber is 1:(2-3).
[0012] Furthermore, the ratio of the depth of the cell culture chamber to the diameter of the top of the cell culture chamber is 1:(4-6).
[0013] Furthermore, the inner wall of the cell culture chamber and the cell planting area are coated with an anti-slip coating, which can prevent the cells from moving or losing during the treatment process.
[0014] Furthermore, the culture dish body is made of a transparent material resistant to high-pressure shock waves, such as glass or high-performance plastic, to ensure that it is not deformed or damaged in a high-pressure shock wave environment.
[0015] Furthermore, the cell culture dish comprises a culture dish cover covering the top of the culture dish body, and the size of the culture dish cover is adapted to the top of the culture dish body.
[0016] Furthermore, the culture dish cover is detachably connected to the culture dish body, so that the conical shock wave probe can be placed on the inner wall of the cell culture chamber and directly aimed at the cell planting area for shock wave treatment.
[0017] Furthermore, the inner ring of the culture dish cover is provided with a sealing structure adapted to the culture dish body to ensure sealing during cell culture.
[0018] Compared with the prior art, the utility model has the following beneficial effects:
[0019] (1) The cell culture dish of the utility model adopts an inverted truncated cone-shaped cell culture chamber, which can naturally focus the shock wave, so that the shock wave energy is concentrated in the cell planting area, and the shock wave energy is utilized to the greatest extent to achieve precise cell treatment.
[0020] (2) The inner wall of the cell culture chamber and the cell planting area of the utility model are coated with an anti-slip coating, which can effectively prevent the cells from moving or losing during the treatment process. At the same time, the high-pressure, high-temperature and corrosion-resistant culture dish material ensures the stability and safety of the culture dish in a high-pressure shock wave environment.
[0021] (3) The culture dish cover of the utility model adopts a detachable design, which is convenient for cell treatment experiments and allows the shock wave probe to pass through and directly act on the cell planting area, avoiding the problem of uneven treatment caused by holding the probe to achieve precise focusing treatment.
[0022] (4) The cell culture dish of the utility model has an ingenious and reasonable structure, is easy to use and operate, and can make the most of the focused shock wave. BRIEF DESCRIPTION OF THE DRAWINGS
[0023] Figure 1 This is a schematic diagram of the overall structure of the cell culture dish of the present utility model.
[0024] Figure 2This is a top view of the cell culture dish of Example 2 of the utility model.
[0025] Figure 3 This is a side view of the cell culture dish of Example 4 of the present utility model.
[0026] Description of the markings in the figure:
[0027] 1- culture dish body, 2- cell culture chamber, 21- cell planting area, 3- culture dish cover. DETAILED DESCRIPTION
[0028] The present invention is described in detail below in conjunction with the accompanying drawings and specific embodiments. This embodiment is implemented based on the technical solution of the present invention, and provides a detailed implementation method and a specific operation process, but the protection scope of the present invention is not limited to the following embodiments.
[0029] In the present utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as a limitation on the present utility model; the terms "first", "second", and "third" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance; in addition, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or an indirect connection through an intermediate medium, or it can be a communication between the two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.
[0030] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.
[0031] Embodiment 1:
[0032] This embodiment provides a cell culture dish for treating cells with focused shock waves. Figure 1As shown, the cell culture dish of this embodiment includes a cylindrical culture dish body 1. The culture dish body 1 is provided with a truncated cone-shaped cell culture chamber 2, which is an inverted truncated cone structure with a wide top and a narrow bottom, and can form a natural focusing surface. The bottom of the cell culture chamber 2 is a horizontal cell planting area 21. The probe of the focused shock wave can be placed on the inner wall of the cell culture chamber 2, and the cells in the cell planting area 21 can be directly subjected to shock wave treatment.
[0033] Embodiment 2:
[0034] This embodiment provides a cell culture dish for focusing shock wave treatment of cells, which includes a cylindrical culture dish body 1. The culture dish body 1 is provided with a truncated cone-shaped cell culture chamber 2, which is an inverted truncated cone structure with a wide top and a narrow bottom, and the bottom of the cell culture chamber 2 is a horizontal cell planting area 21.
[0035] like Figure 2-3 As shown, the cell planting area 21 of this embodiment is arranged concentrically with the top of the cell culture chamber 2, and the cell planting area 21 is located in the center of the culture dish body 1. The diameter ratio of the cell planting area 21 to the top of the cell culture chamber 2 is 1:(2-3), and the depth of the cell culture chamber 2 to the diameter ratio of the top of the cell culture chamber 2 is 1:(4-6).
[0036] Embodiment 3:
[0037] This embodiment provides a cell culture dish for treating cells with focused shock waves.
[0038] In order to improve the stability and safety in the high-pressure shock wave environment, the difference from Example 2 is that the inner wall of the cell culture chamber 2 and the cell planting area 21 of this embodiment are coated with an anti-slip coating. The anti-slip coating can effectively prevent the cells from moving or losing during the shock wave treatment process. The culture dish body 1 is made of transparent glass or plastic material that is resistant to high-pressure shock waves to ensure the stability and safety of the cell culture dish in the high-pressure shock wave environment.
[0039] Embodiment 4:
[0040] This embodiment provides a cell culture dish for treating cells with focused shock waves. The cell culture dish of this embodiment includes a culture dish cover 3 covering the top of the culture dish body 1 , and the size of the culture dish cover 3 matches the top of the culture dish body 1 .
[0041] The culture dish cover 3 of this embodiment is detachably connected to the culture dish body 1, so that the culture dish cover 3 can be easily removed for shock wave treatment. The inner ring of the culture dish cover 3 is provided with a rubber sealing structure adapted to the top of the culture dish body 1 to ensure sealing during cell culture.
[0042] Embodiment 5:
[0043] The present embodiment provides a specific cell culture dish for focusing shock wave treatment of cells and a use process. The cell-specific culture dish of the present embodiment includes a transparent cylindrical culture dish body 1 and a detachable culture dish cover 3 arranged on the top of the culture dish body 1. The culture dish body 1 is provided with an inverted truncated cone-shaped cell culture chamber 2, the bottom of the cell culture chamber 2 is narrower and the top is wider, and a natural focusing surface is formed between the bottom and the top. A circular cell planting area 21 is arranged in the center of the culture dish body 1 for planting cells to be treated. The culture dish cover 3 is provided with a sealing structure adapted to the culture dish body 1 to ensure the sealing of the culture dish during the shock wave treatment process, and the sealing structure can ensure the safety of cell culture. At the same time, the design of the culture dish cover 3 allows the conical shock wave probe to pass through and directly act on the cell planting area 21 to achieve precise focusing treatment.
[0044] Furthermore, the inner wall of the culture dish body 1 and the cell planting area 21 are both provided with an anti-slip coating to prevent the cells from moving or losing during the treatment process. In addition, the material of the culture dish is selected from a transparent material that is resistant to high pressure, high temperature, and corrosion, such as glass or special plastic, to ensure that it will not be deformed or damaged in a high-pressure shock wave environment.
[0045] When using the cell culture dish of this embodiment to perform shock wave treatment on cells, first place the culture dish body 1 on the experimental table and remove the detachable culture dish cover 3. Plant the cells to be treated in the cell planting area 2, paying attention to the uniformity of cell distribution. Then place the conical shock wave probe on the conical slope of the cell culture chamber 2, and adjust the probe position so that it is aligned with the cell planting area 21. Start the shock wave treatment device, set the treatment parameters according to the experimental requirements, and start cell treatment.
[0046] In the above embodiments, the size and shape of the culture dish body 1 can be adjusted as needed to meet the requirements of different experiments. At the same time, the position and size of the cell planting area 21 can also be adjusted as needed to optimize the experimental results.
[0047] The above description of the embodiments is to facilitate the understanding and use of the utility model by those skilled in the art. It is obvious that those familiar with the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the utility model is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the utility model without departing from the scope of the utility model should be within the scope of protection of the utility model.
Claims
1. A cell culture dish for treating cells with focused shock waves, characterized in that: It comprises a cylindrical culture dish body (1); The culture dish body (1) is provided with a truncated cone-shaped cell culture chamber (2). The cell culture chamber (2) is a structure with a wide top and a narrow bottom. The bottom of the cell culture chamber (2) is a horizontal cell planting area (21).
2. A cell culture dish for treating cells with focused shock waves according to claim 1, characterized in that: The cell planting area (21) and the top of the cell culture chamber (2) are arranged in a concentric circle.
3. A cell culture dish for treating cells with focused shock waves according to claim 2, characterized in that: The cell planting area (21) is located in the center of the culture dish body (1).
4. A cell culture dish for treating cells with focused shock waves according to claim 2, characterized in that: The diameter ratio between the cell planting area (21) and the top of the cell culture chamber (2) is 1:(2-3).
5. A cell culture dish for treating cells with focused shock waves according to claim 1, characterized in that: The ratio of the depth of the cell culture chamber (2) to the diameter of the top of the cell culture chamber (2) is 1:(4-6).
6. A cell culture dish for treating cells with focused shock waves according to claim 1, characterized in that: The inner wall of the cell culture chamber (2) and the cell planting area (21) are both coated with an anti-slip coating.
7. A cell culture dish for treating cells with focused shock waves according to claim 1, characterized in that: The culture dish body (1) is made of a transparent material that is resistant to high-pressure shock waves.
8. A cell culture dish for treating cells with focused shock waves according to claim 1, characterized in that: The cell culture dish comprises a culture dish cover (3) covering the top of the culture dish body (1); the culture dish cover (3) is adapted to the size of the top of the culture dish body (1).
9. A cell culture dish for treating cells with focused shock waves according to claim 8, characterized in that: The culture dish cover (3) is detachably connected to the culture dish body (1).
10. The cell culture dish for treating cells with focused shock waves according to claim 8, characterized in that: The inner ring of the culture dish cover (3) is provided with a sealing structure adapted to the top of the culture dish body (1).