Constant temperature machine for stable secretion promotion of stem cell exosome

Abstract

The utility model relates to the technical field of constant-temperature machines, and discloses a stem cell exosome stable secretion promoting constant-temperature machine which comprises a constant-temperature machine body, idler wheels fixedly installed at the bottom of the constant-temperature machine body, a handle fixedly installed on the front face of the constant-temperature machine body and an installation assembly arranged in the constant-temperature machine body. The mounting assembly comprises a mounting mechanism fixedly mounted on the inner wall of the constant-temperature machine body, the mounting mechanism is connected with a fixing mechanism, by arranging the mounting mechanism, the bearing plate and the fixing frame can be conveniently mounted, so that a test tube containing the exosome is borne, constant-temperature storage of the exosome is achieved through the constant-temperature machine body, and the test tube is convenient to use. And by arranging the fixing mechanism, a test tube containing the exosome can be clamped through a clamping block, so that the stability of the test tube in the constant temperature machine body in the transportation process is improved, and the exosome storage effect is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of thermostats, in particular to a thermostat for promoting the stable secretion of exosomes of stem cells. Background Art

[0002] Stem cells are a type of cells with unlimited or immortal self-renewal capabilities and can produce at least one type of highly differentiated progeny cells. In the process of promoting the secretion of stem cell exosomes, a constant temperature machine is used to provide a stable environment for the secretion of stem cell exosomes.

[0003] Since exosomes are easily degradable, in the prior art, during the constant temperature culture of exosomes, the exosomes are usually placed in a test tube, and then the test tube is stored in a constant temperature machine. During the transportation of the constant temperature machine, since there is no device to fix the test tube inside the constant temperature machine, the test tube is easily shaken during transportation, thereby reducing the stability and activity of the exosomes inside, affecting the yield and quality of the exosomes, and reducing the culture effect of the constant temperature machine on the exosomes. For this reason, we proposed a constant temperature machine for stabilizing and promoting the secretion of stem cell exosomes. Utility Model Content

[0004] The purpose of the utility model is to provide a constant temperature machine for promoting the stable secretion of stem cell exosomes, which solves the problems raised in the above-mentioned background technology.

[0005] In order to achieve the above-mentioned purpose, the utility model provides the following technical solutions: a constant temperature machine for promoting the stable secretion of stem cell exosomes, comprising a constant temperature machine body;

[0006] A roller fixedly mounted on the bottom of the thermostat body;

[0007] A cabinet door is hingedly mounted on the front of the thermostat body;

[0008] A handle fixedly mounted on the front of the thermostat body;

[0009] And an installation component is arranged inside the thermostat body, the installation component includes a mounting mechanism fixedly installed on the inner wall of the thermostat body, and the mounting mechanism is connected to a fixing mechanism.

[0010] Preferably, the mounting mechanism comprises a fixing frame fixedly mounted on the inner wall of the thermostat body, the inner wall of the fixing frame is slidably connected with a bearing plate, the side wall of the bearing plate is fixedly mounted with a pull plate, a positioning groove is provided on the top of the pull plate, a rectangular frame is fixedly mounted on the top of the fixing frame, a connecting spring is fixedly mounted on the top of the inner wall of the rectangular frame, the other end of the connecting spring is fixedly mounted on a T-block, the outer wall of the T-block is slidably connected with the inner wall of the rectangular frame, a positioning column is fixedly mounted on the bottom of one end of the T-block, the bottom outer wall of the positioning column is matched with the inner wall of the positioning groove, and the mounting mechanism is provided When the load-bearing plate and the fixed frame need to be assembled before the test tube containing the exosomes needs to be stored, the operator can pull the T-block upwards to squeeze the connecting spring, and then insert the load-bearing plate into the fixed frame to make the side wall of the pull plate fit with the side wall of the fixed frame, and then release the T-block to make the reaction force of the connecting spring reset the T-block and the positioning column, so that the positioning column is inserted into the positioning groove to limit the pull plate, so as to install and fix the load-bearing plate and the fixed frame, thereby improving the stability of the load-bearing plate in the process of carrying the test tube containing the exosomes.

[0011] Preferably, the fixing mechanism comprises a motor fixedly mounted on the top of the carrying plate, the output end of the motor is fixedly mounted with a screw rod, the outer wall of the screw rod is threadedly connected to the mounting plate, the other end of the inner wall of the mounting plate is slidably connected to a limiting column, the bottom of the limiting column is fixedly connected to the top of the carrying plate, the top of the mounting plate is fixedly mounted with a U-shaped block, the inner wall of the U-shaped block is fixedly mounted with a cross column, the outer wall of the cross column is slidably connected to the limiting block, the side wall of the limiting block is fixedly connected with a reset spring, the other end of the reset spring is fixedly connected to the inner wall of the U-shaped block, the side wall of the limiting block is fixedly mounted with a clamping block, the bottom of the clamping block is slidably connected to the top of the mounting plate. By setting the fixing mechanism, when the mounting plate is installed, the operator can adjust the distance between the mounting plate and the carrying plate according to the height of the test tube, which is convenient for replacement. The test tube is effectively clamped and fixed. At this time, the operator can start the motor to cause the screw to rotate. During the rotation of the screw, the limiting column limits the mounting plate, which will cause the mounting plate to move up and down along the outer wall of the screw, so as to facilitate the adjustment of the height of the mounting plate. When the spacing between the mounting plate and the carrier plate is adjusted, the operator can insert the test tube containing exosomes into the mounting plate so that the bottom of the test tube contacts the bottom of the inner wall of the carrier plate. During the insertion of the test tube, the outer wall of the test tube will squeeze the two clamping blocks to both sides. When the test tube is inserted, the two clamping blocks will be compressed by the limiting block under the action of the reset spring, prompting the clamping block to clamp the test tube, thereby avoiding shaking of the test tube during the transportation of the thermostat body, reducing the stability and activity of the exosomes, and affecting the yield and quality of the exosomes.

[0012] Preferably, there are four rollers, which are of equal size and are fixedly installed at four corners of the bottom of the thermostat body at equal distances. Through this arrangement, the thermostat body can be easily transported by the four rollers.

[0013] Preferably, there are two rectangular frames, the two rectangular frames are equal in size, and the two rectangular frames are symmetrically distributed on both sides of the top of the fixed frame along the center plane of the fixed frame.

[0014] Preferably, the inner wall of the limit block is matched with the outer wall of the cross column, and the side wall of the limit block is fixedly connected to the end face of the clamping block.

[0015] The utility model provides a constant temperature machine for promoting the stable secretion of stem cell exosomes. The constant temperature machine for promoting the stable secretion of stem cell exosomes has the following beneficial effects:

[0016] (1) The constant temperature machine for promoting the stable secretion of exosomes from stem cells is provided with an installation mechanism. When the load plate and the fixed frame need to be assembled before the test tube containing the exosomes needs to be stored, the operator can pull the T-block upward to cause the T-block to squeeze the connecting spring, and then insert the load plate into the fixed frame to cause the side wall of the pull plate to fit with the side wall of the fixed frame. Then, the operator releases the T-block to cause the reaction force of the connecting spring to reset the T-block and the positioning column, and causes the positioning column to be inserted into the positioning groove, so as to limit the pull plate, thereby realizing the installation and fixing of the load plate and the fixed frame, thereby improving the stability of the load plate in the process of carrying the test tube containing the exosomes;

[0017] (2) The constant temperature machine for promoting the stable secretion of exosomes from stem cells is provided with a fixing mechanism. After the mounting plate is installed, the operator can adjust the distance between the mounting plate and the mounting plate according to the height of the test tube, so as to more effectively clamp and fix the test tube. At this time, the operator can turn on the motor to cause the screw to rotate. During the rotation of the screw, the mounting plate is limited by the limiting column, which causes the mounting plate to move up and down along the outer wall of the screw, so as to facilitate the adjustment of the height of the mounting plate. After the spacing between the mounting plate and the mounting plate is adjusted, the operator can insert the test tube containing the exosomes into the mounting plate, so that the bottom of the test tube contacts the bottom of the inner wall of the mounting plate. During the insertion of the test tube, the outer wall of the test tube squeezes the two clamping blocks to both sides. After the insertion of the test tube is completed, the two clamping blocks are compressed by the limiting block under the action of the return spring, so that the clamping blocks clamp the test tube, so as to avoid the shaking of the test tube during the transportation of the constant temperature machine body, thereby reducing the stability and activity of the exosomes and affecting the yield and quality of the exosomes. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1This is a schematic diagram of the three-dimensional structure of a thermostatic machine for promoting the stable secretion of stem cell exosomes according to the utility model;

[0019] Figure 2 This is a schematic diagram of the structure of the internal installation components of a constant temperature machine for promoting the stable secretion of stem cell exosomes according to the utility model;

[0020] Figure 3 This is a schematic diagram of the structure of the internal installation mechanism of a constant temperature machine for promoting the stable secretion of stem cell exosomes according to the utility model;

[0021] Figure 4 This is a partial enlarged view of the A direction of a thermostatic machine for promoting the stable secretion of stem cell exosomes according to the utility model;

[0022] Figure 5 This is a schematic diagram of the structure of the internal fixing mechanism of a constant temperature machine for promoting the stable secretion of stem cell exosomes according to the utility model;

[0023] Figure 6 This is a partial enlarged view of the B direction of a constant temperature machine for promoting the stable secretion of stem cell exosomes in the utility model.

[0024] In the figure: 1. thermostat body; 2. roller; 3. cabinet door; 4. handle; 5. installation assembly; 51. installation mechanism; 511. fixing frame; 512. bearing plate; 513. pull plate; 514. positioning groove; 515. rectangular frame; 516. connecting spring; 517. T-block; 518. positioning column; 52. fixing mechanism; 521. motor; 522. screw rod; 523. installation plate; 524. limiting column; 525. U-block; 526. horizontal column; 527. limiting block; 528. reset spring; 529. clamping block. DETAILED DESCRIPTION

[0025] In order to have a clearer understanding of the technical features, purposes and effects of the present utility model, the specific implementation methods of the present utility model are now described with reference to the accompanying drawings.

[0026] Example 1

[0027] A preferred embodiment of the thermostatic machine for promoting the stable secretion of stem cell exosomes provided by the utility model is as follows: Figures 1 to 6 As shown: a constant temperature machine for promoting the stable secretion of stem cell exosomes, comprising a constant temperature machine body 1;

[0028] A roller 2 is fixedly mounted on the bottom of the thermostat body 1;

[0029] A cabinet door 3 is hingedly mounted on the front of the thermostat body 1;

[0030] A handle 4 fixedly mounted on the front of the thermostat body 1;

[0031] And an installation component 5 is arranged inside the thermostat body 1, the installation component 5 includes a mounting mechanism 51 fixedly installed on the inner wall of the thermostat body 1, the mounting mechanism 51 is connected to the fixing mechanism 52, and by setting the mounting mechanism 51, it is convenient to install the carrying plate 512 and the fixing frame 511, so as to realize the carrying of the test tube containing the exosomes, and realize the constant temperature storage of the exosomes through the thermostat body 1, and by setting the fixing mechanism 52, the test tube containing the exosomes can be clamped by the clamping block 529, so as to improve the stability of the internal test tube during the transportation of the thermostat body 1, thereby improving the storage effect of the exosomes.

[0032] The mounting mechanism 51 includes a fixed frame 511 fixedly mounted on the inner wall of the thermostat body 1, the inner wall of the fixed frame 511 is slidably connected with a bearing plate 512, the side wall of the bearing plate 512 is fixedly mounted with a pull plate 513, the top of the pull plate 513 is provided with a positioning groove 514, a rectangular frame 515 is fixedly mounted on the top of the fixed frame 511, a connecting spring 516 is fixedly mounted on the top of the inner wall of the rectangular frame 515, a T-block 517 is fixedly mounted on the other end of the connecting spring 516, the outer wall of the T-block 517 is slidably connected with the inner wall of the rectangular frame 515, a positioning column 518 is fixedly mounted on the bottom of one end of the T-block 517, and the bottom outer wall of the positioning column 518 is adapted to the inner wall of the positioning groove 514.

[0033] In this embodiment, by providing the mounting mechanism 51, when the test tube containing the exosomes needs to be stored, the carrying plate 512 and the fixing frame 511 need to be assembled. At this time, the operator can pull the T-block 517 upward to cause the T-block 517 to squeeze the connecting spring 516, and then insert the carrying plate 512 into the fixing frame 511 to cause the side wall of the pulling plate 513 to fit with the side wall of the fixing frame 511. Then, by releasing the T-block 517, the reaction force of the connecting spring 516 is caused to reset the T-block 517 and the positioning column 518, and the positioning column 518 is caused to be inserted into the positioning groove 514, so as to limit the pulling plate 513, so as to realize the installation and fixation of the carrying plate 512 and the fixing frame 511, thereby improving the stability of the carrying plate 512 in the process of carrying the test tube containing the exosomes.

[0034] Example 2

[0035] On the basis of Example 1, a preferred embodiment of a constant temperature machine for promoting the stable secretion of exosomes of stem cells provided by the utility model is as follows: Figures 1 to 6As shown: the fixing mechanism 52 includes a motor 521 fixedly mounted on the top of the supporting plate 512, a screw rod 522 is fixedly mounted on the output end of the motor 521, the outer wall of the screw rod 522 is threadedly connected to a mounting plate 523, the inner wall of the other end of the mounting plate 523 is slidably connected to a limiting column 524, the bottom of the limiting column 524 is fixedly connected to the top of the supporting plate 512, a U-shaped block 525 is fixedly mounted on the top of the mounting plate 523, a cross column 526 is fixedly mounted on the inner wall of the U-shaped block 525, the outer wall of the cross column 526 is slidably connected to a limiting block 527, a side wall of the limiting block 527 is fixedly connected to a return spring 528, the other end of the return spring 528 is fixedly connected to the inner wall of the U-shaped block 525, a side wall of the limiting block 527 is fixedly mounted to a clamping block 529, and the bottom of the clamping block 529 is slidably connected to the top of the mounting plate 523.

[0036] In this embodiment, by setting the fixing mechanism 52, after the mounting plate 512 is installed, the operator can adjust the distance between the mounting plate 523 and the mounting plate 512 according to the height of the test tube, so as to clamp and fix the test tube more effectively. At this time, the operator can turn on the motor 521 to cause the screw rod 522 to rotate. During the rotation of the screw rod 522, the mounting plate 523 is caused to move up and down along the outer wall of the screw rod 522 due to the limiting effect of the limiting column 524 on the mounting plate 523, so that the height of the mounting plate 523 can be adjusted. 2, the operator can insert the test tube containing exosomes into the interior of the mounting plate 523 so that the bottom of the test tube contacts the bottom of the inner wall of the carrier plate 512. During the insertion of the test tube, the outer wall of the test tube will squeeze the two clamping blocks 529 to both sides. When the test tube is inserted, the two clamping blocks 529 will be compressed by the limit block 527 under the action of the reset spring 528, prompting the clamping block 529 to clamp the test tube, thereby preventing the test tube from shaking during the transportation of the thermostat body 1, reducing the stability and activity of the exosomes, and affecting the yield and quality of the exosomes.

[0037] Furthermore, there are four rollers 2 , which are equal in size and are fixedly installed at four corners of the bottom of the thermostat body 1 at equal distances. Through this arrangement, the thermostat body 1 can be easily transported by the four rollers 2 .

[0038] Furthermore, there are two rectangular frames 515 , the two rectangular frames 515 are equal in size, and the two rectangular frames 515 are symmetrically distributed on both sides of the top of the fixed frame 511 along the center plane thereof.

[0039] In addition, the inner wall of the limit block 527 is matched with the outer wall of the cross column 526 , and the side wall of the limit block 527 is fixedly connected to the end surface of the clamping block 529 .

[0040] Working principle: When the test tube containing exosomes needs to be stored, the carrier plate 512 and the fixed frame 511 need to be assembled. At this time, the operator can pull the T-block 517 upward to cause the T-block 517 to squeeze the connecting spring 516, and then insert the carrier plate 512 into the fixed frame 511 to cause the side wall of the pull plate 513 to fit with the side wall of the fixed frame 511. Then, by releasing the T-block 517, the reaction force of the connecting spring 516 is caused to reset the T-block 517 and the positioning column 518, and the positioning column 518 is caused to be inserted into the positioning groove 514, so as to limit the pull plate 513, so as to realize the installation and fixation of the carrier plate 512 and the fixed frame 511, thereby improving the stability of the carrier plate 512 in the process of carrying the test tube containing exosomes. After the installation of the carrier plate 512 is completed, the operator can adjust the distance between the mounting plate 523 and the carrier plate 512 according to the height of the test tube, so as to facilitate more effective loading of the test tube. The screw rod 522 is clamped and fixed. At this time, the operator can turn on the motor 521 to cause the screw rod 522 to rotate. During the rotation of the screw rod 522, due to the limiting effect of the limiting column 524 on the mounting plate 523, the mounting plate 523 will be prompted to move up and down along the outer wall of the screw rod 522, so as to facilitate the adjustment of the height of the mounting plate 523. After the spacing between the mounting plate 523 and the supporting plate 512 is adjusted, the operator can insert the test tube containing exosomes into the mounting plate 523 so that the bottom of the test tube contacts the bottom of the inner wall of the supporting plate 512. During the insertion of the test tube, the outer wall of the test tube will squeeze the two clamping blocks 529 to both sides. After the insertion of the test tube is completed, under the action of the return spring 528, the two clamping blocks 529 will be compressed through the limiting block 527, prompting the clamping block 529 to clamp the test tube, thereby avoiding the shaking of the test tube during the transportation of the thermostat body 1, reducing the stability and activity of the exosomes, and affecting the yield and quality of the exosomes.

[0041] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A thermostat for promoting the stable secretion of stem cell exosomes, comprising a thermostat body (1); A roller (2) fixedly mounted on the bottom of the thermostat body (1); A cabinet door (3) mounted on the front side of the thermostat body (1) by means of hinged movement; A handle (4) fixedly mounted on the front of the thermostat body (1); and a mounting assembly (5) arranged inside the thermostat body (1), characterized in that: The mounting assembly (5) comprises a mounting mechanism (51) fixedly mounted on the inner wall of the thermostat body (1), and the mounting mechanism (51) is connected to a fixing mechanism (52).

2. A constant temperature machine for promoting the stable secretion of stem cell exosomes according to claim 1, characterized in that: The mounting mechanism (51) comprises a fixed frame (511) fixedly mounted on the inner wall of the thermostat body (1); the inner wall of the fixed frame (511) is slidably connected to a bearing plate (512); a pull plate (513) is fixedly mounted on the side wall of the bearing plate (512); a positioning groove (514) is provided on the top of the pull plate (513); a rectangular frame (515) is fixedly mounted on the top of the fixed frame (511); a connecting spring (516) is fixedly mounted on the top of the inner wall of the rectangular frame (515); the other end of the connecting spring (516) is fixedly mounted on a T-shaped block (517); the outer wall of the T-shaped block (517) is slidably connected to the inner wall of the rectangular frame (515); a positioning column (518) is fixedly mounted on the bottom of one end of the T-shaped block (517); the outer wall of the bottom of the positioning column (518) is adapted to the inner wall of the positioning groove (514).

3. A constant temperature machine for promoting the stable secretion of stem cell exosomes according to claim 1, characterized in that: The fixing mechanism (52) comprises a motor (521) fixedly mounted on the top of the bearing plate (512); a screw rod (522) is fixedly mounted on the output end of the motor (521); an outer wall of the screw rod (522) is threadedly connected to a mounting plate (523); an inner wall of the other end of the mounting plate (523) is slidably connected to a limiting column (524); the bottom of the limiting column (524) is fixedly connected to the top of the bearing plate (512); a U-shaped block (524) is fixedly mounted on the top of the mounting plate (523); 5), a transverse column (526) is fixedly installed on the inner wall of the U-shaped block (525), the outer wall of the transverse column (526) is slidably connected to a limit block (527), the side wall of the limit block (527) is fixedly connected to a return spring (528), the other end of the return spring (528) is fixedly connected to the inner wall of the U-shaped block (525), the side wall of the limit block (527) is fixedly installed with a clamping block (529), and the bottom of the clamping block (529) is slidably connected to the top of the mounting plate (523).

4. A constant temperature machine for promoting the stable secretion of stem cell exosomes according to claim 1, characterized in that: There are four rollers (2), the four rollers (2) are of equal size, and the four rollers (2) are fixedly installed at four corners of the bottom of the thermostat body (1) at equal distances.

5. A constant temperature machine for promoting the stable secretion of stem cell exosomes according to claim 2, characterized in that: There are two rectangular frames (515), the two rectangular frames (515) are equal in size, and the two rectangular frames (515) are symmetrically distributed on both sides of the top of the fixed frame (511) along the center plane.

6. A constant temperature machine for promoting the stable secretion of stem cell exosomes according to claim 3, characterized in that: The inner wall of the limit block (527) is matched with the outer wall of the cross column (526), ​​and the side wall of the limit block (527) is fixedly connected to the end surface of the clamping block (529).

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