Ultraviolet protein detection device of Y-shaped optical fiber LED light source

Abstract

The utility model discloses an ultraviolet protein detection device of a Y-shaped optical fiber LED light source. The ultraviolet protein detection device comprises a power supply module, an ultraviolet spectrum detection unit, a flow cell and an upper computer, the power supply module comprises a 12V direct current circuit, a 5V direct current circuit and a 3.3 V voltage stabilizing circuit; the ultraviolet spectrum detection unit comprises an LED drive circuit, an LED lamp, a pre-amplification circuit, a filter circuit, an AD conversion circuit and a microcontroller MCU. The LED driving circuit drives the LED lamp, the LED lamp emits two beams of ultraviolet light, one beam serves as an absorption light source and irradiates the ultraviolet detector through the flow cell, and the other beam serves as a reference light source and directly irradiates the ultraviolet detector; and the upper computer is connected with the MCU and is used for receiving data, drawing a spectrogram on a screen, editing the spectrogram and storing the data. The ultraviolet protein detection device with the Y-shaped optical fiber LED light source has the advantages that the anti-interference capability of the detection device can be improved, the measurement precision and sensitivity are higher, the stability and the adaptability are good, and the like.

Description

Technical Field

[0001] The utility model relates to a protein detection technology, in particular to an ultraviolet protein detection device with a Y-shaped optical fiber LED light source. Background Art

[0002] Proteins are biological macromolecules that make up cells and are the main bearers of life activities. A typical eukaryotic cell contains thousands of proteins, whose molecular structures, physical and chemical properties and functions vary greatly. With the development of life sciences and the deepening of research, researchers have realized that it is far from enough to rely solely on genome analysis to explain the phenomena and essence of life activities. Only by conducting in-depth research on proteins and proteomics can we better grasp the phenomena and laws of life and reveal its essence.

[0003] To study proteins, we must first obtain high-purity, biologically active, and relatively stable target proteins, and proteins exist in the form of complex mixtures in tissues or cells. Therefore, efficient protein purification technology and analysis methods are one of the foundations and keys of protein research.

[0004] In order to study the specific functions and structures of proteins, researchers must separate and purify recombinant proteins from organisms. Protein purification methods mainly utilize the similarities and differences between different recombinant proteins. Non-protein substances can be removed based on the similarities between proteins, and then the target recombinant protein can be separated and purified based on the differences between proteins. Protein purification systems are mainly used for the separation and purification of biological products such as monoclonal antibodies, recombinant proteins, vaccines, biochemical drugs, antibiotics, natural products and polysaccharides. They are the core key equipment in all basic research fields of protein science and the production and research and development of protein antibody vaccine drugs.

[0005] Protein purification is roughly divided into two stages: coarse separation and fine purification. The coarse separation stage mainly separates the target protein from other cell components such as RNA and DNA, and the commonly used method is ammonium sulfate precipitation. The purpose of the fine purification stage is to distinguish the target protein from other proteins with similar size and physical and chemical properties. The most indispensable device in the protein purification system is the UV protein detection device. The UV protein detection device is mainly used to detect whether there is protein in the purified and separated sample. The UV protein detection device is the most widely used detector in the protein purification system.

[0006] In the prior art, ultraviolet protein detection devices generally use a single optical path system. The Chinese utility model patent CN201620571620.5 discloses a device for quickly determining the state of microbial outer membrane proteins. The device includes a rheostat, a light source, a collimator, a cuvette, a cuvette holder, an integrating sphere, and a fiber optic spectrometer; along the optical axis, the light source, the collimator, the cuvette holder, and the incident hole of the integrating sphere are arranged in sequence, and the collimator, the cuvette holder, and the incident hole of the integrating sphere are closely connected; the cuvette is arranged on the cuvette holder, and the width of the cuvette is not less than the diameter of the incident hole of the integrating sphere; the integrating sphere is connected to the fiber optic spectrometer via an optical fiber. This single-fiber detection device, with its single optical path detection system, has the disadvantages of low measurement accuracy, poor stability and environmental adaptability. Summary of the invention

[0007] The utility model aims to avoid the deficiencies in the above-mentioned prior art and provides an ultraviolet protein detection device with a Y-shaped optical fiber LED light source to improve the anti-interference ability, measurement accuracy and sensitivity, stability and adaptability of the detection device.

[0008] The utility model adopts the following technical solutions to solve the technical problems.

[0009] The utility model discloses a Y-shaped optical fiber LED light source ultraviolet protein detection device, which mainly comprises a power module, an ultraviolet spectrum detection unit 1, a flow cell and a host computer 2;

[0010] The power supply module includes a 12V DC circuit, a 5V DC circuit and a 3.3V voltage stabilizing circuit;

[0011] The ultraviolet spectrum detection unit includes an LED driving circuit, an LED lamp 3, a preamplifier circuit, a filter circuit, an AD conversion circuit and a microcontroller MCU; the LED driving circuit drives the LED lamp, and the LED lamp emits two beams of ultraviolet light, one beam is used as an absorption light source to irradiate the ultraviolet detector 4 through the circulation pool 5, and the other beam is used as a reference light source to directly irradiate the ultraviolet detector 4;

[0012] The host computer is connected to the microcontroller MCU for data reception, screen spectrum drawing, spectrum editing, and data storage.

[0013] The structural features of the Y-shaped optical fiber LED light source ultraviolet protein detection device of the utility model are also:

[0014] Furthermore, the two beams of ultraviolet light are Y-shaped.

[0015] Furthermore, the microcontroller MCU is a STM32F103VET6 chip.

[0016] Furthermore, the ultraviolet detector 4 is a silicon photodiode.

[0017] Furthermore, the preamplifier circuit includes an operational amplifier U2, a silicon photodiode VD1, a connection terminal U3, resistors R1-R2, capacitors C3-C9, and inductors L2-L3.

[0018] Furthermore, the AD conversion circuit includes an AD conversion chip U4, a connection terminal CN2, resistors R5-R12, diodes D2-D5, capacitors C11-C19 and a crystal oscillator X1.

[0019] Furthermore, the AD conversion chip U4 is AD7192.

[0020] Furthermore, the 12V DC circuit includes a connection terminal CN1, a fuse F1, capacitors C20-C22, resistors R3-R4, a diode D6 and a transistor Q1.

[0021] Furthermore, the 5V DC circuit includes a voltage stabilizing chip U1, a capacitor C10, capacitors C23-C24, an inductor L4, a diode D7, a voltage stabilizing tube D8 and a switch SW1.

[0022] Furthermore, the 3.3V voltage stabilizing circuit includes a load switch U6, resistors R13-R15, capacitors C25-C28, a light emitting diode LED1, and a three-terminal voltage stabilizing chip U7.

[0023] Compared with the prior art, the beneficial effects of the utility model are embodied in:

[0024] The utility model discloses an ultraviolet protein detection device of a Y-shaped optical fiber LED light source, comprising a power module, an ultraviolet spectrum detection unit, a circulation pool, and a host computer; the power module comprises a 12V DC circuit, a 5V DC circuit, and a 3.3V voltage stabilizing circuit; the ultraviolet spectrum detection unit comprises an LED driving circuit, an LED lamp, a preamplifier circuit, a filter circuit, an AD conversion circuit, and a microcontroller MCU; the LED driving circuit drives the LED lamp, and the LED lamp emits two beams of ultraviolet light, one beam is used as an absorption light source to irradiate the ultraviolet detector through the circulation pool, and the other beam is used as a reference light source to directly irradiate the ultraviolet detector; the host computer is connected to the microcontroller MCU and is used for data reception, screen spectrum drawing, spectrum editing, and data storage.

[0025] The utility model discloses an ultraviolet protein detection device with a Y-shaped optical fiber LED light source, which has the following characteristics.

[0026] 1. High sensitivity: The UV absorption detector has high sensitivity, which can reach the ppt level, which makes it very valuable in trace analysis.

[0027] 2. Low noise: The UV absorption detector has a low noise level, which helps improve the accuracy of the measurement.

[0028] 3. Wide linear range: The UV absorption detector has a wide linear range, which means it can accurately measure concentrations over a large range.

[0029] 4. Good selectivity: The UV absorption detector has good selectivity and can be used to detect substances with specific UV absorption characteristics.

[0030] 5. It is not very sensitive to changes in ambient temperature, mobile phase composition and flow rate fluctuations: this allows the UV absorption detector to be used for both isocratic and gradient elution.

[0031] 6. Wide range of applications: Any substance that absorbs ultraviolet light can be used, making ultraviolet absorption detectors the most widely used in liquid chromatography.

[0032] The Y-shaped optical fiber LED light source ultraviolet protein detection device of the utility model has the advantages of being able to improve the anti-interference ability of the detection device, having higher measurement accuracy and sensitivity, and good stability and adaptability. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 This is a schematic diagram of an ultraviolet protein detection device with a Y-shaped optical fiber LED light source of the utility model.

[0034] Figure 2 The utility model is a schematic diagram of the light path of a UV protein detection device with a Y-shaped optical fiber LED light source.

[0035] Figure 3 This is a schematic structural diagram of ultraviolet light passing through a flow cell of an ultraviolet protein detection device with a Y-shaped optical fiber LED light source according to the utility model.

[0036] Figure 4 This is a circuit structure framework diagram of an ultraviolet protein detection device with a Y-shaped optical fiber LED light source of the utility model.

[0037] Figure 5 This is a MCU communication structure diagram of an ultraviolet protein detection device with a Y-shaped optical fiber LED light source of the utility model.

[0038] Figure 6 This is a pin diagram of the MCU of an ultraviolet protein detection device with a Y-shaped optical fiber LED light source of the utility model.

[0039] Figure 7 The utility model discloses a circuit diagram of a preamplifier circuit of an ultraviolet protein detection device with a Y-shaped optical fiber LED light source.

[0040] Figure 8 The utility model discloses a circuit diagram of an AD conversion circuit of an ultraviolet protein detection device with a Y-shaped optical fiber LED light source.

[0041] Fig. 9 The utility model discloses a circuit diagram of a 12V DC circuit of an ultraviolet protein detection device with a Y-shaped optical fiber LED light source.

[0042] Fig.10 The utility model discloses a circuit diagram of a 5V DC circuit of an ultraviolet protein detection device with a Y-shaped optical fiber LED light source.

[0043] Fig.11 The utility model discloses a circuit diagram of a 3.3V voltage stabilizing circuit of an ultraviolet protein detection device with a Y-shaped optical fiber LED light source.

[0044] The present invention is further described below through specific implementation methods in conjunction with the accompanying drawings. DETAILED DESCRIPTION

[0045] See also Figures 1 to 11 The utility model discloses a Y-shaped optical fiber LED light source ultraviolet protein detection device, which mainly includes a power module, an ultraviolet spectrum detection unit 1, a flow cell and a host computer 2;

[0046] The power supply module includes a 12V DC circuit, a 5V DC circuit and a 3.3V voltage stabilizing circuit;

[0047] The ultraviolet spectrum detection unit includes an LED driving circuit, an LED lamp 3, a preamplifier circuit, a filter circuit, an AD conversion circuit and a microcontroller MCU; the LED driving circuit drives the LED lamp, and the LED lamp emits two beams of ultraviolet light, one beam is used as an absorption light source to irradiate the ultraviolet detector 4 through the circulation pool 5, and the other beam is used as a reference light source to directly irradiate the ultraviolet detector 4;

[0048] The host computer is connected to the microcontroller MCU for data reception, screen spectrum drawing, spectrum editing, and data storage.

[0049] like Figure 1 to Figure 4 The utility model is a Y-shaped optical fiber LED light source ultraviolet protein detection device, the LED lamp of the ultraviolet spectrum detection unit is used as the ultraviolet light source. Figure 2 and Figure 3 When the sample after protein purification and separation flows through the flow cell, the ultraviolet light source emits ultraviolet light. The Y-type optical fiber 6 evenly divides the emitted light of the LED lamp into two beams of ultraviolet light sources: one beam is used as an absorption light source to irradiate the ultraviolet detector of the ultraviolet spectrum detection unit through the flow cell, and the other beam is used as a reference light source to directly irradiate the ultraviolet detector of the ultraviolet spectrum detection unit. Figure 3The two ends of the circulation pool 5 are connected to the water inlet and outlet pipes through the liquid path connector 7. One end outlet of the Y-type optical fiber 6 is connected to the circulation pool 7.

[0050] like Figure 1 and Figure 2 As shown, the ultraviolet detector 4 is two silicon photodiodes, which are used for detecting two beams of ultraviolet light sources respectively. The silicon photodiode works in zero bias mode, that is, photovoltaic mode. In this mode, the silicon photodiode can work very accurately and linearly. Under zero bias conditions, there is no dark current, the diode noise is equivalent to the thermal noise of the shunt circuit resistor, and there is only the thermal noise of Rj (node ​​shunt resistor), and the linearity and sensitivity are the highest. Therefore, the photovoltaic mode is very suitable for high-precision detection. On the contrary, under reverse bias conditions, there is a dark current that generates an additional noise source, which is not suitable for high-precision detection.

[0051] like Figure 4 , the ultraviolet detector obtains two electrical signals proportional to the transmitted light through the photoelectric converter. The electrical signal is connected to the preamplifier circuit for signal amplification, and then filtered through the filter circuit to remove noise. The signal after filtering is proportional to the transmitted light. The AD conversion circuit uses a dual-channel AD converter. The filtered signal is sent to the dual-channel AD converter, and the dual-channel AD converter outputs a digital signal to the microcontroller MCU; the MCU connects the control end and the output end of the AD converter, and connects the signal processing interface circuit of the host computer. The host computer is a computer workstation used for data reception, screen drawing, spectrum editing, and data storage. The absorbance data of the liquid in the circulation pool is displayed in real time on the screen of the host computer.

[0052] During specific implementation, the two beams of ultraviolet light are Y-shaped.

[0053] like Figure 2 , two beams of ultraviolet light are emitted from the LED lamp, and the two rays are emitted outward in a Y shape. The characteristic wavelength emitted by the ultraviolet light source is a light source of 280nm. The Y-shaped optical fiber 6 is used in the utility model to realize dual-light path detection, which has the following advantages compared with a single light path: (1) Strong anti-interference ability: The dual light path setting can effectively resist various types of interference, including environmental noise, equipment errors, etc.; (2) Improve measurement accuracy: By using two light paths for measurement, the error that may be introduced by a single light path can be reduced, thereby improving the measurement accuracy; (3) Enhance the stability of the system: The dual light path setting can improve the stability of the system, because the failure of one light path will not affect the normal operation of the other light path; (4) Improve measurement sensitivity: In some cases, the dual light path setting can improve the sensitivity of measurement; (5) Eliminate background noise: The dual light path setting can effectively eliminate background noise and improve the accuracy of measurement results.

[0054] In a specific implementation, the microcontroller MCU is a STM32F103VET6 chip.

[0055] like Figure 5 and Figure 6 , MCU realizes SPI communication with two AD conversion circuits through SPI interface (i.e. pins PA4, PA5, PA6, PA7), one of which obtains absorption light sampling signal, and the other obtains reference light sampling signal. MCU receives the digital signals of the two AD conversion circuits and sends the two digital signals to the host computer through 485 communication.

[0056] like Figure 6 , the microcontroller U5 is connected with inductor L1, capacitor C1 and capacitor 2, and the connection relationship of each component is as follows Figure 7 As shown. STM32F103VET6 is a 32-bit ARM Cortex-M3 microcontroller launched by STMicroelectronics. STM32F103VET6 integrates the ARM Cortex-M3 core with a maximum frequency of 72MHz. It also integrates 128KB Flash memory and 20KB SRAM memory. STM32F103VET6 has a variety of peripheral interfaces, including SPI, I2C, USART, and USB. STM32F103VET6 integrates a variety of timers, including general timers, advanced control timers, watchdog timers, and real-time clocks. STM32F103VET6 has low power consumption characteristics, with an operating power supply voltage of 3V to 5.25V and a power consumption of 4.35mA. Other peripheral circuits of MCU, such as crystal oscillator circuits and reset circuits, are all conventional circuit structures.

[0057] During specific implementation, the ultraviolet detector 4 is a silicon photodiode.

[0058] The ultraviolet detector model is a silicon photodiode S1336-5BQ, which is suitable for precise photometric measurement in the ultraviolet to near-infrared band.

[0059] In a specific implementation, the preamplifier circuit includes an operational amplifier U2, a silicon photodiode VD1, a connection terminal U3, resistors R1-R2, capacitors C3-C9, and inductors L2-L3.

[0060] like Figure 7 This is the circuit diagram of the preamplifier circuit. The connection relationship of each component is as follows Figure 7As shown. U2 of the preamplifier circuit is the ultraviolet detector. In the utility model, a silicon photodiode S1336-5BQ is used as the ultraviolet detector. Since the output of the ultraviolet detector is a weak current signal, it is necessary to add a preamplifier circuit to amplify the signal to prevent excessive noise from drowning the current signal and add a resistor-capacitor circuit for filtering. The amplifier circuit adopts a transconductance mode, which can convert the current signal of the ultraviolet detector into a voltage signal amplified in the same proportion; R1 and C3 are used as a resistor-capacitor low-pass filter circuit to filter out high-frequency noise in the signal, and output the amplified voltage signal through U3, and the output amplified signal is transmitted to the AD conversion circuit.

[0061] The model of operational amplifier U2 is ADA4530-1-1ARZ-R7. ADA4530-1 is a fA-class (10-15A) input bias current operational amplifier suitable for use as an electrometer, and it also integrates a guard ring buffer. Its operating voltage range is 4.5V to 16V, and it can be powered by conventional 5V and 10V single power supplies as well as ±2.5V and ±5V dual power supply systems.

[0062] In a specific implementation, the AD conversion circuit includes an AD conversion chip U4, a connection terminal CN2, resistors R5-R12, diodes D2-D5, capacitors C11-C19 and a crystal oscillator X1.

[0063] like Figure 8 This is the circuit diagram of the AD conversion circuit. The connection relationship between the components is as follows Figure 8 As shown. The AD conversion circuit has the main function of converting the collected analog signal into a digital signal that can be recognized by the computer. The output signals of the two preamplifier circuits are connected to AIN1 and AIN2 of the AD conversion chip U4 on one side and AIN3 and AIN4 of the AD conversion chip U4 on the other side. AIN2 and AIN4 are grounded respectively. Using a differential circuit and using AIN1-AIN2 and AIN3-AIN4 as the input signals of the AD conversion circuit can enhance the anti-interference ability of the signal and effectively suppress electromagnetic interference EMI. The converted digital signal is output by the DOUT pin of the AD conversion chip U4.

[0064] During specific implementation, the AD conversion chip U4 is AD7192.

[0065] The utility model adopts a 24-bit high-precision AD conversion chip AD7192. AD7192 is a low-noise complete analog front end suitable for high-precision measurement applications. Its main features and functional principles are as follows: (1) Low noise: AD7192 integrates a low-noise, 24-bit Σ-Δ analog-to-digital converter (ADC); (2) Direct input of small signals: The on-chip low-noise gain stage means that small signals can be directly input; (3) Flexible configuration: This device can be configured as two differential inputs or four pseudo-differential inputs; (4) Channel sequencer: The on-chip channel sequencer can enable multiple channels, and AD7192 performs conversions on each enabled channel in sequence, which can simplify communication with the device; (5) Clock source: The on-chip 4.92MHz clock can be used as the clock source of the ADC; alternatively, an external clock or crystal oscillator can also be used; (6) Output data rate: The output data rate of the device can vary from 4.7Hz to 4.8kHz; (7) Zero delay feature: For applications that require all conversions to be settled, AD7192 has a zero delay feature.

[0066] In a specific implementation, the 12V DC circuit includes a connection terminal CN1, a fuse F1, capacitors C20-C22, resistors R3-R4, a diode D6 and a transistor Q1.

[0067] like Fig. 9 This is the circuit diagram of a 12V DC circuit. The connection relationship between the components is as follows Fig. 9 As shown. There are two external power supplies, one is 12V from the CN1 port, and the other is 5V from the 5V-USB port. You can choose any one of them as the main power supply. The external 12V power supply is introduced from the CN1 port and divided into two paths. One path passes through the fuse F1 and is filtered by the filter capacitor as the 12V power supply input for the control circuit board; the other path is divided into 5V through R3 and R4, and is connected to the G pole and D pole of the transistor Q1 respectively with the 5V-USB. The transistor Q1 is a field effect transistor, which is used as a power management switch in the circuit, so that only one power supply is turned on at the same time, and the other is disconnected. When the external power supply is supplied, the USB power supply will be disconnected to prevent current injection and protect the computer USB port.

[0068] In a specific implementation, the 5V DC circuit includes a voltage stabilizing chip U1, a capacitor C10, capacitors C23-C24, an inductor L4, a diode D7, a voltage stabilizing tube D8 and a switch SW1.

[0069] like Fig.10 This is the circuit diagram of a 5V DC circuit. The connection relationship between the components is as follows Fig.10As shown. The voltage regulator chip U1 model is LM2596S-5.0, which is used as a step-down DC-DC switching regulator in the circuit. It can convert the input 12V voltage into a stable 5V output voltage. The C24 electrolytic capacitor is used to filter the high-frequency noise of the input voltage; the inductor L4 is used for energy storage and filtering, D7 can prevent reverse voltage, and C23 filters the output voltage to provide a stable DC voltage. SW1 is TK-6580A-1, which is a key switch and serves as the power-on switch of the main controller.

[0070] The LM2596 series voltage regulator is a monolithic integrated circuit with all the effective functions of a step-down switching regulator, capable of driving a 3A load and having excellent line and load regulation performance. The device provides fixed output voltages of 3.3V, 5V, and 12V and adjustable output voltages. The LM2596S-5.0 is a high-performance DC-DC regulated power converter. Using the LM2596S-5.0 is very simple and only requires a few external components to achieve the function. It has a built-in frequency compensation module, which makes the frequency stable and has good anti-interference ability. Users can achieve precise adjustment of the output voltage by adjusting the duty cycle linear range of 0 to 100%. The parameters of the LM2596S-5.0 are VIN (input voltage) of 40V, VOUT (output voltage) of 5V, and IOUT (output current) of 10mA.

[0071] In a specific implementation, the 3.3V voltage stabilizing circuit includes a load switch U6, resistors R13-R15, capacitors C25-C28, a light emitting diode LED1, and a three-terminal voltage stabilizing chip U7.

[0072] like Fig.11 This is the circuit diagram of the 3.3V voltage regulator circuit. The connection relationship of each component is as follows Fig.11 As shown. Load switch U6 is MT9700, which acts as overcurrent protection for 3.3V output and limits the current to within 2A. MT9700 is a cost-effective low-voltage single P MOSFET load switch. The input range of this switch is 2.4V to 5.5V, which is very suitable for 3V and 5V systems. The switch RDS(ON) is low, 80mΩ, meeting the USB voltage drop requirements. MT9700 is also protected by thermal overload, which limits power consumption and connection temperature. The current limit threshold is programmed with a resistor from SET to ground. In the on state of the switch, the quiescent supply current is typically 15μA. In the off state, the supply current drops to less than 1μA. U7 is a three-terminal voltage regulator integrated circuit LM7805, which inputs 5V voltage, converts the 5V input voltage into 3.3V voltage and outputs it to the load switch U6 and the light-emitting diode LED1. The main advantages are easy to use, low price, overheat protection, good linearity and high temperature stability.

[0073] The utility model is a Y-shaped optical fiber LED light source ultraviolet protein detection device. Based on the Lambert-Beer law, the detection method, light source design, and optical path conversion are designed. Experiments have proved that the utility model can complete ultraviolet protein detection, and can form a complete protein purification system when equipped with components such as chromatography columns, constant flow pumps, and fraction collectors. The utility model uses a 280nm wavelength ultraviolet light source to perform real-time synchronous detection of the absorbance of the circulation pool during the chromatography process and designs a computer software workstation. A rich absorbance spectrum is drawn.

[0074] The utility model provides a Y-shaped optical fiber LED light source ultraviolet protein detection device to achieve the following functions.

[0075] 1. Automatically adjust the absorbance to 0.000 and the transmittance to 100%.

[0076] 2. Dual optical path synchronous detection

[0077] 3. Range: Absorbance (A): 0-4.000

[0078] 4. Linearity: 5%

[0079] 5. Data acquisition, computer interface (COM port and USB port) and software workstation, integrated system integration of detection, acquisition and software workstation;

[0080] 6. The upper computer workstation has the functions of data receiving, screen drawing, spectrum editing and data saving.

[0081] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

[0082] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A Y-shaped optical fiber LED light source ultraviolet protein detection device, characterized in that: It comprises a power module, an ultraviolet spectrum detection unit (1), a circulation cell and a host computer (2); The power supply module includes a 12V DC circuit, a 5V DC circuit and a 3.3V voltage stabilizing circuit; The ultraviolet spectrum detection unit comprises an LED driving circuit, an LED lamp (3), a preamplifier circuit, a filter circuit, an AD conversion circuit and a microcontroller MCU; the LED driving circuit drives the LED lamp, and the LED lamp emits two beams of ultraviolet light, one beam as an absorption light source irradiates the ultraviolet detector (4) through a circulation pool (5), and the other beam as a reference light source directly irradiates the ultraviolet detector (4); The host computer is connected to the microcontroller MCU for data reception, screen spectrum drawing, spectrum editing, and data storage.

2. The ultraviolet protein detection device of a Y-shaped optical fiber LED light source according to claim 1, characterized in that: The two beams of ultraviolet light are Y-shaped.

3. The ultraviolet protein detection device of a Y-shaped optical fiber LED light source according to claim 1, characterized in that: The microcontroller MCU is a STM32F103VET6 chip.

4. The ultraviolet protein detection device of a Y-shaped optical fiber LED light source according to claim 1, characterized in that: The ultraviolet detector is a silicon photodiode.

5. The ultraviolet protein detection device of a Y-shaped optical fiber LED light source according to claim 1, characterized in that: The preamplifier circuit includes an operational amplifier U2, a silicon photodiode VD1, a connection terminal U3, resistors R1-R2, capacitors C3-C9, and inductors L2-L3.

6. The ultraviolet protein detection device of a Y-shaped optical fiber LED light source according to claim 1, characterized in that: The AD conversion circuit includes an AD conversion chip U4, a connection terminal CN2, resistors R5-R12, diodes D2-D5, capacitors C11-C19 and a crystal oscillator X1.

7. The ultraviolet protein detection device of a Y-shaped optical fiber LED light source according to claim 6, characterized in that: The AD conversion chip U4 is AD7192.

8. The ultraviolet protein detection device of a Y-shaped optical fiber LED light source according to claim 1, characterized in that: The 12V DC circuit includes a connection terminal CN1, a fuse F1, capacitors C20-C22, resistors R3-R4, a diode D6 and a transistor Q1.

9. The ultraviolet protein detection device of a Y-shaped optical fiber LED light source according to claim 1, characterized in that: The 5V DC circuit includes a voltage stabilizing chip U1, a capacitor C10, capacitors C23-C24, an inductor L4, a diode D7, a voltage stabilizing tube D8 and a switch SW1.

10. The ultraviolet protein detection device of a Y-shaped optical fiber LED light source according to claim 1, characterized in that: The 3.3V voltage stabilizing circuit includes a load switch U6, resistors R13-R15, capacitors C25-C28, a light emitting diode LED1, and a three-terminal voltage stabilizing chip U7.

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