Quantitative preparation device for cell culture medium

Abstract

The utility model discloses a cell culture medium quantitative preparation device which comprises a movable frame, a mixing tank and a tank cover, a workbench is fixedly installed on the top face of the movable frame, a sterile operation box is fixedly installed on the top face of the workbench, an installation frame is fixedly installed in the center of the top face of the workbench, and the mixing tank is fixedly installed on the installation frame. A stirring shaft is mounted in the center of an inner cavity of the mixing tank, a plurality of stirring rods are uniformly and fixedly mounted on the stirring shaft, the bottom end of the stirring shaft is connected with the output end of a stirring motor, and a scraping plate is fixedly mounted on the stirring shaft and abuts against the inner wall and the bottom surface of the mixing tank. The device has the beneficial effects that the scraping plate, the mixing tank, the tank cover, the cleaning spray head and the ultraviolet sterilizing lamp are arranged, so that residual materials adhered to the inner wall of the mixing tank can be conveniently scraped, a user can conveniently clean and disinfect the inner cavity of the mixing tank, the possibility that a prepared cell culture medium is polluted is reduced, and the device is more convenient to use and higher in practicability.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture medium preparation, and more specifically, to a device for quantitatively preparing cell culture medium. Background Art

[0002] A cell culture medium is not only a basic substance that supplies nutrients to cells and promotes cell reproduction and proliferation during cell culture, but also the living environment for cell growth and reproduction. According to its physical state, the culture medium can be divided into liquid culture medium and solid culture medium. The liquid culture medium is used for large-scale industrial production and research work on basic theories such as physiological metabolism. The cultivation of cells needs to be carried out in a culture dish, and it has the advantages of simple operation, stable structure and good use effect in actual use.

[0003] The prior art discloses a device for preparing NK cell culture medium with the application number: CN202122378342.5. Although the above utility model can drive a stirring paddle by a stirring motor to mix and stir the raw materials of the cell culture medium quantitatively introduced into the mixing tank evenly during use, when the mixed cell culture medium is exported, it is difficult to ensure that the culture medium adhered to the inner wall of the mixing tank is quickly exported, and the residual materials remaining in the inner cavity of the mixing tank need to open the flange cover for cleaning and washing, and the operation is relatively cumbersome. Moreover, the inner cavity of the mixing tank cannot be disinfected to prevent bacteria from breeding and contaminating the subsequent preparation of cell culture medium, and the practicability is relatively general. In view of the disadvantages of the above utility model, those skilled in the art have proposed a device for quantitatively preparing cell culture medium. Summary of the Utility Model

[0004] (1) Technical Problems to be Solved

[0005] Aiming at the deficiencies of the prior art, the utility model provides a device for quantitatively preparing cell culture medium, which has the advantages of being convenient for scraping the residual materials adhered to the inner wall of the mixing tank, being convenient for the user to clean and disinfect the inner cavity of the mixing tank, reducing the possibility of the prepared cell culture medium being contaminated, being more convenient to use and having stronger practicability, so as to solve the problems in the above background art.

[0006] (2) Technical Solutions

[0007] To achieve the advantages of facilitating the scraping of the residual material adhering to the inner wall of the mixing tank, facilitating the user to clean and disinfect the inner cavity of the mixing tank, reducing the possibility of the prepared cell culture medium being contaminated, being more convenient to use and having stronger practicability, the specific technical solution adopted by the present utility model is as follows: A cell culture medium quantitative preparation device, including a mobile rack, a mixing tank and a tank cover. The top surface of the mobile rack is fixedly installed with a workbench, and the top surface of the workbench is fixedly installed with a sterile operation box. The center of the top surface of the workbench is fixedly installed with a mounting rack, and a mixing tank is fixedly installed on the mounting rack. A stirring shaft is installed in the center of the inner cavity of the mixing tank, and a number of stirring rods are evenly and fixedly installed on the stirring shaft. And the bottom end of the stirring shaft is connected to the output end of a stirring motor. A scraper is fixedly installed on the stirring shaft, and the scraper is in contact with the inner wall and the bottom surface of the mixing tank. An installation screw hole is opened on the top surface of the mixing tank, and a number of installation bolts are installed on the tank cover. The bottom surface of the tank cover is fixedly installed with an annular water distribution tank, and the inner cavity of the annular water distribution tank is connected and communicated with a sterile water pipe. And a water pump is installed on the sterile water pipe. A number of cleaning spray heads are evenly installed on the bottom surface of the annular water distribution tank. A number of ultraviolet sterilization lamps are evenly installed on the bottom surface of the tank cover.

[0008] Further, a number of support plates are evenly and fixedly installed on the inner wall of the sterile operation box, and auxiliary material storage tanks are fixedly installed on the support plates. And one end of a quantitative preparation pipe is connected and communicated with the bottom of the inner cavity of the auxiliary material storage tank, and the other end of the quantitative preparation pipe is connected and communicated with the inner cavity of the mixing tank. And a small metering pump is installed on the quantitative preparation pipe.

[0009] Further, an air filtration component is installed in the inner cavity of the sterile operation box, and an air extraction pipe is installed on the air filtration component.

[0010] Further, a control panel is installed on the side wall of the mobile rack, and the control panel is electrically connected to the stirring motor, the small metering pump, the water pump and the ultraviolet sterilization lamp.

[0011] Further, one side of the bottom surface of the mixing tank is connected and communicated with a discharge pipe, and a discharge valve is installed on the discharge pipe.

[0012] Further, universal wheels are evenly installed at the four corners of the bottom surface of the mobile rack.

[0013] Further, the central axes of the mixing tank, the tank cover, the stirring shaft and the annular water distribution tank are on the same straight line.

[0014] (III) Beneficial effects

[0015] Compared with the prior art, the present utility model provides a cell culture medium quantitative preparation device, which has the following beneficial effects:

[0016] (1) This utility model is provided with a support plate, an auxiliary material storage tank, a small metering pump, a quantitative preparation pipe and a mixing tank. The top surface of the provided mobile frame is fixedly installed with a workbench, and the top surface of the workbench is fixedly installed with a sterile operation box. The center of the top surface of the provided workbench is fixedly installed with a mounting frame, and a mixing tank is fixedly installed on the mounting frame. A number of support plates are evenly and fixedly installed on the inner wall of the provided sterile operation box, and an auxiliary material storage tank is fixedly installed on the support plate. One end of a quantitative preparation pipe is connected and communicated with the bottom of the inner cavity of the auxiliary material storage tank. The other end of the provided quantitative preparation pipe is connected and communicated with the inner cavity of the mixing tank, and a small metering pump is installed on the quantitative preparation pipe. By using the provided number of small metering pumps, the preparation raw materials in the corresponding auxiliary material storage tanks can be accurately introduced into the inner cavity of the mixing tank, so that the user does not need to manually use a pipette for manual preparation. It not only has simple operation, improves the preparation efficiency of the cell culture medium, but also does not cause consumable losses to the pipette tips, saving resources. In addition, since the auxiliary material storage tank and the quantitative preparation pipe are both located above the bottom of the inner cavity of the mixing tank, various raw materials transported will not remain in the inner cavity of the quantitative preparation pipe under the action of gravity, ensuring the accuracy of the preparation of various raw materials of the cell culture medium, with stronger practicability. A stirring shaft is installed in the center of the inner cavity of the provided mixing tank, and a number of stirring rods are evenly and fixedly installed on the stirring shaft. The bottom end of the stirring shaft is connected to the output end of a stirring motor. When the stirring motor is started through the control panel, the stirring shaft can drive the stirring rods to evenly mix and stir various preparation raw materials, and the obtained culture medium material can be discharged through the discharge pipe into the culture medium, realizing the one-time quantitative preparation of the cell culture medium.

[0017] (2) The present utility model is provided with a scraper, a mixing tank, a tank cover, a cleaning spray head and an ultraviolet sterilization lamp. As described above, the prepared cell culture medium material is led out to the culture medium through the discharge pipe, and this process is carried out inside the sterile operation box cavity. Connect the air extraction pipe to the air extraction pump, and cooperate with the air filtration component to ensure the sterile operation environment inside the sterile operation box. The fixed installation of the scraper on the stirring shaft is provided, and the provided scraper abuts against the inner wall and the bottom surface of the mixing tank, which can quickly scrape and discharge the material remaining on the inner wall of the mixing tank, enabling it to fully discharge, reducing the material residue in the mixing tank cavity, and at the same time improving the quantitative preparation accuracy. The top surface of the provided mixing tank is provided with installation screw holes, and several installation bolts are installed on the tank cover. By using the cooperation of the installation bolts and the installation screw holes, the tank cover can be installed on the mixing tank. The bottom surface of the provided tank cover is fixedly installed with an annular water distribution tank, and the inner cavity of the annular water distribution tank is communicated with a sterile water pipe, and a water pump is installed on the sterile water pipe. When the device is not used for preparing the cell culture medium, the user can introduce sterile water into the inner cavity of the annular water distribution tank through the sterile water pipe, and finally spray it out through several cleaning spray heads installed on the bottom surface of the annular water distribution tank to realize the automatic cleaning of the inner cavity of the mixing tank and the stirring rod. After the cleaning is completed, the user can turn on the ultraviolet sterilization lamp installed on the bottom surface of the tank cover through the control panel to realize the continuous disinfection and sterilization of the inner cavity of the mixing tank, reducing the possibility of the next prepared cell culture medium being contaminated, making it more convenient to use and more practical. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions in the embodiments of the present utility model or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the following described drawings are only some embodiments of the present utility model. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0019] Figure 1 is a schematic structural diagram of a cell culture medium quantitative preparation device according to an embodiment of the present utility model;

[0020] Figure 2 is according to an embodiment of the present utility model Figure 1 enlarged view of part A;

[0021] Figure 3 is according to an embodiment of the present utility model Figure 1 enlarged view of part B;

[0022] Figure 4 is according to an embodiment of the present utility model Figure 1 enlarged view of part C;

[0023] Figure 5It is a three-dimensional view of a scraper and a stirring rod of a cell culture medium quantitative preparation device according to an embodiment of the present utility model.

[0024] In the figure:

[0025] 1. Moving frame; 2. Workbench; 3. Aseptic operation box; 4. Small metering pump; 5. Support plate; 6. Air filtration component; 7. Quantitative preparation pipe; 8. Air extraction pipe; 9. Auxiliary material storage tank; 10. Stirring rod; 11. Stirring shaft; 12. Scraper; 13. Universal wheel; 14. Mixing tank; 15. Stirring motor; 16. Discharge pipe; 17. Discharge valve; 18. Tank cover; 19. Aseptic water pipe; 20. Annular water equalizing tank; 21. Water pump; 22. Cleaning spray head; 23. Ultraviolet sterilization lamp; 24. Installation screw hole; 25. Installation bolt; 26. Installation frame. Specific embodiments

[0026] To further illustrate each embodiment, the present utility model provides attached drawings. These attached drawings are a part of the disclosure content of the present utility model. They are mainly used to illustrate the embodiments and can cooperate with the relevant descriptions in the specification to explain the operating principle of the embodiments. With reference to these contents, those of ordinary skill in the art should be able to understand other possible implementation manners and the advantages of the present utility model. The components in the figures are not drawn to scale, and similar component symbols are usually used to represent similar components.

[0027] According to an embodiment of the present utility model, a cell culture medium quantitative preparation device is provided.

[0028] Now, the present utility model will be further described in combination with the attached drawings and specific embodiments, as Figures 1-5As shown in the figure, a cell culture medium quantitative preparation device according to an embodiment of the present invention includes a mobile rack 1, a mixing tank 14 and a tank cover 18. A workbench 2 is fixedly installed on the top surface of the mobile rack 1, and a sterile operation box 3 is fixedly installed on the top surface of the workbench 2. An installation rack 26 is fixedly installed in the center of the top surface of the workbench 2, and a mixing tank 14 is fixedly installed on the installation rack 26. A stirring shaft 11 is installed in the center of the inner cavity of the mixing tank 14, and a plurality of stirring rods 10 are evenly and fixedly installed on the stirring shaft 11. The bottom end of the stirring shaft 11 is connected to the output end of a stirring motor 15. A scraper 12 is fixedly installed on the stirring shaft 11, and the scraper 12 abuts against the inner wall and bottom surface of the mixing tank 14. An installation screw hole 24 is opened on the top surface of the mixing tank 14, and a plurality of installation bolts 25 are installed on the tank cover 18. A ring-shaped uniform water tank 20 is fixedly installed on the bottom surface of the tank cover 18, and a sterile water pipe 19 is connected to the inner cavity of the ring-shaped uniform water tank 20 in a communicating manner. A water pump 21 is installed on the sterile water pipe 19. A plurality of cleaning nozzles 22 are evenly installed on the bottom surface of the ring-shaped uniform water tank 20. A plurality of ultraviolet sterilization lamps 23 are evenly installed on the bottom surface of the tank cover 18, which facilitates scraping the residual material adhering to the inner wall of the mixing tank 14, and facilitates the user to clean and disinfect the inner cavity of the mixing tank 14, reducing the possibility of the prepared cell culture medium being contaminated, making it more convenient to use and more practical.

[0029] In one embodiment, a plurality of support plates 5 are evenly and fixedly installed on the inner wall of the sterile operation box 3, and auxiliary material storage tanks 9 are fixedly installed on the support plates 5. One end of a quantitative preparation pipe 7 is connected to the bottom of the inner cavity of the auxiliary material storage tank 9 in a communicating manner, and the other end of the quantitative preparation pipe 7 is connected to the inner cavity of the mixing tank 14 in a communicating manner. A small metering pump 4 is installed on the quantitative preparation pipe 7, which can accurately introduce the preparation raw materials in the corresponding auxiliary material storage tanks 9 into the inner cavity of the mixing tank 14 by using the plurality of small metering pumps 4 provided. Thus, it is not necessary for the user to manually use a pipette for manual preparation. It not only has simple operation, improves the preparation efficiency of the cell culture medium, but also does not cause consumable loss to the pipette tips, saving resources.

[0030] In one embodiment, an air filtration component 6 is installed in the inner cavity of the sterile operation box 3, and an air extraction pipe 8 is installed on the air filtration component 6, which can ensure the sterile operation environment in the sterile operation box 3.

[0031] In one embodiment, a control panel is installed on the side wall of the mobile rack 1, and the control panel is electrically connected to the stirring motor 15, the small metering pump 4, the water pump 21 and the ultraviolet sterilization lamp 23. The control circuit of the control panel can be realized by simple programming by those skilled in the art, which belongs to the common knowledge in the art. Only its use is carried out without modification, so the control method and circuit connection will not be described in detail.

[0032] In one embodiment, one side of the bottom surface of the mixing tank 14 is connected and communicated with a discharge pipe 16, and a discharge valve 17 is installed on the discharge pipe 16, which serves to export the prepared cell culture medium material.

[0033] In one embodiment, universal wheels 13 are evenly installed at the four corners of the bottom surface of the moving frame 1, which serves to facilitate movement.

[0034] In one embodiment, the central axes of the mixing tank 14, the tank cover 18, the stirring shaft 11 and the annular water equalizing tank 20 are on the same straight line.

[0035] Working principle: The utility model is provided with a support plate 5, an auxiliary material storage tank 9, a small metering pump 4, a quantitative preparation pipe 7 and a mixing tank 14. The top surface of the moving frame 1 is fixedly installed with a workbench 2, and the top surface of the workbench 2 is fixedly installed with a sterile operation box 3. The center of the top surface of the workbench 2 is fixedly installed with a mounting frame 26, and the mixing tank 14 is fixedly installed on the mounting frame 26. A number of support plates 5 are evenly and fixedly installed on the inner wall of the sterile operation box 3, and the auxiliary material storage tank 9 is fixedly installed on the support plate 5. The bottom of the inner cavity of the auxiliary material storage tank 9 is connected to one end of the quantitative preparation pipe 7. The other end of the quantitative preparation pipe 7 is connected to the inner cavity of the mixing tank 14, and a small metering pump 4 is installed on the quantitative preparation pipe 7. By using a number of small metering pumps 4, the preparation raw materials in the corresponding auxiliary material storage tanks 9 can be accurately introduced into the inner cavity of the mixing tank 14, so that the user does not need to manually use a pipette for manual preparation. This not only simplifies the operation, improves the preparation efficiency of the cell culture medium, but also does not cause consumable losses to the pipette tips, saving resources. In addition, since the auxiliary material storage tank 9 and the quantitative preparation pipe 7 are both located above the bottom of the inner cavity of the mixing tank 14, various raw materials transported will not remain in the inner cavity of the quantitative preparation pipe 7 under the action of gravity, ensuring the accuracy of the preparation of various raw materials for the cell culture medium, and having stronger practicability. A stirring shaft 11 is installed in the center of the inner cavity of the mixing tank 14, and a number of stirring rods 10 are evenly and fixedly installed on the stirring shaft 11. The bottom end of the stirring shaft 11 is connected to the output end of a stirring motor 15. When the stirring motor 15 is started through the control panel, the stirring shaft 11 can drive the stirring rods 10 to uniformly mix and stir various preparation raw materials. The obtained culture medium material can be discharged through the discharge pipe 16 into the culture medium, realizing the primary quantitative preparation of the cell culture medium. In addition, the utility model is provided with a scraper 12, a mixing tank 14, a tank cover 18, a cleaning nozzle 22 and an ultraviolet sterilization lamp 23. As described above, the prepared cell culture medium material is discharged into the culture medium through the discharge pipe 16, and this process is carried out inside the sterile operation box 3. Connect the air extraction pipe 8 to the air extraction pump, and cooperate with the air filtration component 6 to ensure the sterile operation environment inside the sterile operation box 3. A scraper 12 is fixedly installed on the stirring shaft 11. The scraper 12 is in contact with the inner wall and the bottom surface of the mixing tank 14, and can quickly scrape off the materials remaining on the inner wall of the mixing tank 14 for discharging, enabling it to be fully discharged, reducing the material residue in the inner cavity of the mixing tank 14, and at the same time improving the quantitative preparation accuracy. The top surface of the mixing tank 14 is provided with mounting screw holes 24, and a number of mounting bolts 25 are installed on the tank cover 18. By using the cooperation of the mounting bolts 25 and the mounting screw holes 24, the tank cover 18 can be installed on the mixing tank 14. The bottom surface of the tank cover 18 is fixedly installed with an annular water distribution tank 20, and the inner cavity of the annular water distribution tank 20 is connected to a sterile water pipe 19. A water pump 21 is installed on the sterile water pipe 19. When the device is not used for preparing the cell culture medium, the user can introduce sterile water into the inner cavity of the annular water distribution tank 20 through the sterile water pipe 19.Finally, it is sprayed out through several cleaning nozzles 22 installed on the bottom surface of the annular water equalizing tank 20, so as to realize the automatic cleaning of the inner cavity of the mixing tank 14 and the stirring rod 10. After the cleaning is completed, the user can turn on the ultraviolet sterilization lamp 23 installed on the bottom surface of the tank cover 18 through the control panel, so as to realize the continuous disinfection and sterilization of the inner cavity of the mixing tank 14, reduce the possibility of the next prepared cell culture medium being contaminated, and is more convenient to use and has stronger practicability.

[0036] In the present utility model, unless otherwise clearly defined and limited, terms such as "installation", "setting", "connection", "fixation", "rotary connection" and the like shall be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or integrated; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the internal communication of two components or the interaction relationship between two components. Unless otherwise clearly defined, for those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0037] The above are only the preferred embodiments of the present utility model and are not intended to limit the present utility model. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present utility model.

Claims

1. A quantitative preparation device for a cell culture medium, comprising a mobile frame (1), a mixing tank (14) and a tank cover (18), characterized in that: A workbench (2) is fixedly mounted on the top surface of the mobile frame (1), and a sterile operating box (3) is fixedly mounted on the top surface of the workbench (2); a mounting frame (26) is fixedly mounted at the center of the top surface of the workbench (2), and a mixing tank (14) is fixedly mounted on the mounting frame (26); a stirring shaft (11) is mounted at the center of the inner cavity of the mixing tank (14), and a plurality of stirring rods (10) are evenly fixedly mounted on the stirring shaft (11), and the bottom end of the stirring shaft (11) is connected to the output end of a stirring motor (15); a scraper (12) is fixedly mounted on the stirring shaft (11), and the scraper (12) is fixedly mounted on the scraper (12). 2) abutting against the inner wall and bottom surface of the mixing tank (14), the top surface of the mixing tank (14) is provided with a mounting screw hole (24), the tank cover (18) is provided with a plurality of mounting bolts (25), the bottom surface of the tank cover (18) is fixedly provided with an annular water homogenizing tank (20), the inner cavity of the annular water homogenizing tank (20) is connected to a sterile water pipe (19), and a water pump (21) is installed on the sterile water pipe (19), the bottom surface of the annular water homogenizing tank (20) is evenly provided with a plurality of cleaning nozzles (22), and the bottom surface of the tank cover (18) is evenly provided with a plurality of ultraviolet sterilization lamps (23).

2. The cell culture medium quantitative preparation device according to claim 1, characterized in that: A plurality of support plates (5) are evenly and fixedly mounted on the inner wall of the sterile operation box (3), and an auxiliary material storage tank (9) is fixedly mounted on the support plate (5). The bottom of the inner cavity of the auxiliary material storage tank (9) is connected to one end of a quantitative preparation pipe (7), and the other end of the quantitative preparation pipe (7) is connected to the inner cavity of a mixing tank (14), and a small metering pump (4) is mounted on the quantitative preparation pipe (7).

3. The cell culture medium quantitative preparation device according to claim 1, characterized in that: An air filter assembly (6) is installed in the inner cavity of the sterile operation box (3), and an air extraction pipe (8) is installed on the air filter assembly (6).

4. The cell culture medium quantitative preparation device according to claim 1, characterized in that: A control panel is installed on the side wall of the mobile frame (1), and the control panel is electrically connected to the stirring motor (15), the small metering pump (4), the water pump (21) and the ultraviolet sterilization lamp (23).

5. The cell culture medium quantitative preparation device according to claim 1, characterized in that: One side of the bottom surface of the mixing tank (14) is connected to a discharge pipe (16), and a discharge valve (17) is installed on the discharge pipe (16).

6. The cell culture medium quantitative preparation device according to claim 1, characterized in that: Universal wheels (13) are evenly mounted at the four corners of the bottom surface of the movable frame (1).

7. The cell culture medium quantitative preparation device according to claim 1, characterized in that: The central axes of the mixing tank (14), the tank cover (18), the stirring shaft (11) and the annular water homogenizing tank (20) are on the same straight line.

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