Liposome extrusion device

Abstract

The utility model discloses a lipidosome extrusion device which comprises a tank body arranged in a hollow mode in the axial direction and sealing covers arranged at the two axial ends of the tank body respectively, a filter membrane is arranged in the middle of the tank body, and the space in the tank body is divided into two parts through the filter membrane; the sealing cover comprises a fixed part and a rotating part coaxially arranged outside the fixed part in a sleeving mode, the rotating part is fixedly connected with the tank body, and the rotating part can rotate relative to the fixed part. According to the lipidosome extrusion tank, a split type sealing cover structure is adopted, the rotating part and the fixed part are arranged in a relative rotating mode, so that the tank body and the fixed part can form a rotating relation, the partition plate and the ribs are further arranged on the fixed part, and therefore in the extrusion work process in the tank body, the lipidosome can be extruded out of the tank body. And the ribs and the filter membrane can interact by rotating the tank body to stir lipidosome on the surface of the filter membrane, so that accumulation and blockage are effectively prevented, and the efficiency and quality in the extrusion process are ensured.

Description

Technical Field

[0001] The utility model belongs to the technical field of liposome processing, and relates to a liposome extrusion device. Background Art

[0002] Liposomes are vesicles formed by a lipid bilayer membrane and are widely used in fields such as drug delivery, gene carriers, and cosmetics. Usually, it is very difficult to precisely control the particle size of liposomes during preparation, and the quality is uneven. In order to prepare high-quality liposomes, strict filtration and purification are required.

[0003] At present, there are already a variety of liposome extrusion device products on the market, mainly used for extruding and preparing liposomes with uniform particle size, including but not limited to micro hand-push type extruders, tank-type liposome extruders, gas pressurization type extruders, continuous extruders, and so on in various forms. The commonality of these products is that they all need to rely on special filter membranes to extrude liposomes through the membrane. The differences lie in the extrusion power, method, and efficiency. Therefore, even though there are various forms, there is still an important defect widely existing in existing products, that is, due to the membrane-passing method of liposomes and the filter membrane, the problem of filter membrane blockage still exists in existing products. Blockage will lead to a significant reduction in extrusion efficiency, and at the same time, it will also cause excessive extrusion pressure, resulting in situations such as the rupture of liposome vesicles, thus seriously affecting the extrusion quality. Therefore, the blockage problem of liposomes needs to be solved urgently. Summary of the Utility Model

[0004] The utility model aims to overcome the deficiencies of the prior art and provides a liposome extrusion device.

[0005] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0006] A liposome extrusion device includes a tank body that is hollow along the axis, and covers respectively arranged at both axial ends of the tank body. A filter membrane is arranged in the middle of the tank body, and the filter membrane divides the space inside the tank body into two parts; the cover includes a fixed part and a rotating part coaxially sleeved outside the fixed part. The rotating part is fixedly connected to the tank body, and the rotating part can rotate relative to the fixed part. A feeding pipe communicating with the inside of the tank body is arranged on the fixed part. It also includes a partition plate, and ribs are convexly arranged on the surface of the partition plate, and the ribs are in contact with the surface of the filter membrane.

[0007] Furthermore, it also includes a bracket. A first fixing member and a second fixing member are fixedly arranged on the bracket. The two feeding pipes respectively pass through the first fixing member and the second fixing member. Limiting grooves are respectively opened on the first fixing member and the second fixing member, and limiting blocks for extending into the limiting grooves are respectively arranged on the two fixed parts.

[0008] Furthermore, a cylinder is provided on the bracket, a hopper is connected to the feeding pipe on one of the sealing caps, a space for accommodating liposomes is formed in the hopper, a piston is provided in the hopper, and a power output end of the cylinder is connected to the piston.

[0009] Furthermore, the tank body is also provided with a side passage pipe, one end of which is connected to the tank body, and the other end of which is connected to a water pump.

[0010] Furthermore, a sealing rubber ring is sleeved on the outer periphery of the fixed part, and the outer periphery of the sealing rubber ring abuts against the inner peripheral wall of the rotating part.

[0011] Furthermore, the rotating part is threadably connected to the tank body.

[0012] Furthermore, a flange is axially arranged on the inner peripheral wall of the tank body, and the filter membrane is arranged against the flange.

[0013] Furthermore, the partition is provided with a plurality of through holes.

[0014] In summary, the utility model is beneficial in that:

[0015] The liposome extrusion tank of the utility model adopts a split-type sealing structure, so that the rotating part and the fixed part are relatively rotatably arranged, so that the tank body can form a rotational relationship with the fixed part, and further a partition and ribs are arranged on the fixed part, so that during the extrusion work in the tank body, the ribs and the filter membrane can interact by rotating the tank body, stirring the liposomes on the surface of the filter membrane, effectively preventing accumulation and clogging, and ensuring the efficiency and quality of the extrusion process. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic structural diagram of the extrusion device of the utility model.

[0017] Figure 2 for Figure 1 Schematic diagram of the structure of the extrusion tank part.

[0018] Figure 3 for Figure 2 Schematic diagram of the cross-section structure in.

[0019] Figure 4 for Figure 3 Schematic diagram of the structure after the partition is hidden.

[0020] Figure 5 for Figure 3 Schematic diagram of the structure of the middle partition.

[0021] Markings in the figure: 1. Tank body; 11. Filter membrane; 12. Flange; 13. Side through pipe; 21. Fixed part; 211. Sealing rubber ring; 22. Rotating part; 23. Feed pipe; 3. Bracket; 31. First fixing part; 32. Second fixing part; 33. Limiting groove; 34. Limiting block; 4. Partition; 41. Rib; 42. Through hole; 43. Retaining ring; 5. Hopper; 6. Cylinder. DETAILED DESCRIPTION

[0022] The following describes the implementation of the present invention through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific implementations, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict.

[0023] It should be noted that the illustrations provided in the following embodiments are only schematic illustrations of the basic concept of the present invention, and thus the drawings only show components related to the present invention rather than being drawn according to the number, shape and size of components in actual implementation. In actual implementation, the type, quantity and proportion of each component may be changed arbitrarily, and the component layout may also be more complicated.

[0024] All directional indications in the embodiments of the present invention (such as up, down, left, right, front, back, horizontal, vertical...) are only used to explain the relative position relationship, movement status, etc. between the components in a certain specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0025] Due to installation errors and other reasons, the parallel relationship referred to in the embodiments of the present invention may actually be an approximately parallel relationship, and the vertical relationship may actually be an approximately vertical relationship.

[0026] The utility model provides a liposome extrusion device, which is used for preparing high-quality liposomes with balanced particle sizes.

[0027] Specifically, refer to Figure 2 and Figure 3 The liposome extrusion device includes an extrusion tank, which includes a tank body 1 with a hollow cylindrical structure. The tank body 1 is axially through and has openings at both ends. In this embodiment, the axial direction of the tank body 1 is arranged in a vertical direction, so that the two openings of the tank body 1 are arranged upward and downward respectively.

[0028] The two axial ends of the tank body 1 are respectively provided with covers, and the covers close the two end openings of the tank body 1 to form a space for the transmembrane liposomes in the tank body 1. The cover is fixed to the tank body 1 in a threaded connection. Specifically, the inner periphery of the cover is provided with an internal thread, and the outer periphery of the tank body 1 is provided with a matching external thread; the cover is provided with a feeding pipe 23, and the feeding pipe 23 is connected to the tank body 1 and is used for the liposomes to enter and exit the tank body 1.

[0029] In this embodiment, the two sealing covers are respectively arranged at the top and the bottom of the tank body 1, wherein the sealing cover arranged at the top is a feed sealing cover, and the feeding pipe 23 thereon is arranged as a feed pipe, which is used for feeding liposomes into the tank body 1; the sealing cover arranged at the bottom is a discharge sealing cover, and the feeding pipe 23 thereon is arranged as a discharge pipe, which is used for discharging liposomes in the tank body 1 outward, and the feed pipe and the discharge pipe are both arranged along the axial direction of the tank body 1.

[0030] Reference Figure 4 A flange 12 is circumferentially arranged on the inner wall of the tank body 1, and a filter membrane 11 is arranged in the tank body 1. The filter membrane 11 is a circular sheet structure, and its outer periphery forms an interference fit with the inner periphery of the tank body 1. The filter membrane 11 is arranged on the flange 12, so that the flange 12 provides bottom support for the filter membrane 11 to maintain the filter membrane 11 in the middle position of the tank body 1, and at the same time, the filter membrane 11 is kept sealed in the circumferential direction when pressure is applied.

[0031] Reference Figure 1 A hopper 5 is provided on the feed pipe, and a certain space for accommodating the liposomes to be treated is formed in the hopper 5, and a piston for compressing the space is provided in the hopper 5. The action of the piston in the hopper 5 allows the liposomes to be treated to enter the tank body 1 through the feed pipe, and further exerts pressure on the liposomes. A cylinder 6 is provided above the extrusion tank, and the cylinder 6 uses nitrogen or compressed air as a power source input, which acts on the piston to drive it to move, thereby achieving pressurization of the liposomes in the hopper 5 and the tank body 1.

[0032] In order to solve the problem of liposomes being blocked during the membrane passing process, in the present embodiment, the sealing cap is further provided with a split structure, including a rotating part 22 and a fixed part 21, and the rotating part 22 is coaxially arranged with the fixed part 21, and the rotating part 22 is sleeved on the outer periphery of the fixed part 21, so that the rotating part 22 can rotate relative to the fixed part 21 with the axis as the rotation center; wherein, in the present embodiment, it is arranged that the rotating part 22 is fixedly connected with the tank body 1 by a threaded connection, and accordingly, the rotating part 22 and the tank body 1 can rotate relative to the fixed part 21 with the axis as the rotation center.

[0033] Reference Figure 3 and Figure 5 The fixing portion 21 is also provided with a snap ring 43, and the snap ring 43 is snapped on the inner periphery of the fixing portion 21, one end of the snap ring 43 extends toward the direction of the filter membrane 11, and a partition 4 is provided at its end, the partition 4 is arranged relative to one side of the filter membrane 11, and a plurality of ribs 41 are arranged on the surface of the partition 4, the ribs 41 are in a strip-shaped structure and protrude from the surface of the partition 4, the ribs 41 are against the surface of the filter membrane 11, and a certain gap is formed between the surface of the filter membrane 11 and the surface of the partition 4, when the tank body 1 rotates relative to the fixing portion 21, the filter membrane 11 is driven to rotate relative to the partition 4, so that the ribs 41 move on the surface of the filter membrane 11, so as to detach the liposomes accumulated and blocked on the surface of the filter membrane 11, and restore the extrusion filtration effect of the filter membrane 11.

[0034] The partition plate 4 is also provided with a plurality of through holes 42 for the passage of liposomes, and can also block excessive liposomes to prevent excessive liposomes from accumulating on the surface of the filter membrane 11 .

[0035] Preferably, the partition plate 4 is disposed on the fixed portion 21 of the feed cover, or the partition plate 4 is disposed on the fixed portion 21 of the feed cover and the fixed portion 21 of the discharge cover, respectively.

[0036] In order to ensure the sealing effect of the cover with a split structure, a sealing rubber ring 211 is sleeved on the outer periphery of the fixed part 21. When the rotating part 22 is sleeved and installed outside the fixed part 21, the sealing rubber ring 211 is squeezed and deformed to fit tightly against the inner wall of the rotating part 22, thereby forming an effective sealing effect.

[0037] The extrusion device also includes a bracket 3, the extrusion tank is installed in the middle of the bracket 3, the cylinder 6 is installed on the upper part of the bracket 3, and the bracket 3 is provided with a first fixing member 31 and a second fixing member 32. The first fixing member 31 and the second fixing member 32 are both fixed to the bracket 3 by screws. The first fixing member 31 and the second fixing member 32 are respectively arranged on both sides of the extrusion tank, so that the feed pipe and the discharge pipe are respectively installed on the first fixing member 31 and the second fixing member 32. Furthermore, the first fixing member 31 and the second fixing member 32 are respectively provided with limiting grooves 33, and the fixing portion 21 of the feed cover and the fixing portion 21 of the discharge cover are both provided with limiting blocks 34 for extending into the corresponding limiting grooves 33, so that when the extrusion tank is installed on the two fixing members on the bracket 3, the limiting blocks 34 extend into the limiting grooves 33 to limit the rotation of the fixing portion 21 of the feed cover and the fixing portion 21 of the discharge cover, thereby ensuring the stability of the tank body 1 during rotation.

[0038] The filter membrane 11 divides the space in the tank body 1 into two parts, an upper part and an lower part, wherein the upper part is close to the feed cover plate to form an extrusion cavity, and the liposomes are subjected to a certain pressure in the extrusion cavity and are squeezed downward from the filter membrane 11; a side passage 13 is arranged on the periphery of the tank body 1, one end of the side passage 13 is opened to communicate with the extrusion cavity, and the other end is opened to be connected to a water pump through a hose. When the water pump is working, water can be pumped into the extrusion cavity along the side passage 13 to disturb the liposomes in the extrusion cavity and dilute them, thereby avoiding blockage caused by excessive accumulation of liposomes.

[0039] Furthermore, the opening of the side passage 13 connected to the extrusion chamber is set as a straight-line opening, and faces the gap between the partition 4 and the surface of the filter membrane 11, so that the water flow pumped into the extrusion chamber along the side passage 13 can be guided to impact the surface of the filter membrane 11, and during the rotation of the tank body 1, the side passage 13 can be synchronously moved along the circumference of the filter membrane 11, so that the water flow evenly impacts the circumference of the filter membrane 11, and cooperates with the action of the ribs 41 to effectively reduce the accumulation of liposomes on the surface of the filter membrane 11.

[0040] Obviously, the described embodiments are only some embodiments of the utility model, not all embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the utility model.

Claims

1. A liposome extrusion device, characterized in that, The invention comprises a tank body (1) which is hollow in the axial direction, and sealing covers respectively arranged at the two axial ends of the tank body (1); a filter membrane (11) is arranged in the middle of the tank body (1); the filter membrane (11) divides the space in the tank body (1) into two parts; the sealing cover comprises a fixed part (21) and a rotating part (22) which is coaxially sleeved outside the fixed part (21); the rotating part (22) is fixedly connected to the tank body (1); the rotating part (22) can rotate relative to the fixed part (21); the fixed part (21) is provided with a material feeding pipe (23) which is connected to the tank body (1); and the invention also comprises a partition (4); a rib (41) is protruding from the surface of the partition (4); the rib (41) abuts against the surface of the filter membrane (11).

2. A liposome extrusion device according to claim 1, characterized in that, The invention also comprises a bracket (3), wherein a first fixing member (31) and a second fixing member (32) are fixedly arranged on the bracket (3), the two feeding pipes (23) are respectively passed through the first fixing member (31) and the second fixing member (32), the first fixing member (31) and the second fixing member (32) are respectively provided with a limiting groove (33), and the two fixing parts (21) are respectively provided with a limiting block (34) for extending into the limiting groove (33).

3. A liposome extrusion device according to claim 2, characterized in that, The support (3) is provided with a cylinder (6), and the feeding pipe (23) on one of the sealing covers is connected with a hopper (5), a space for accommodating liposomes is formed in the hopper (5), a piston is provided in the hopper (5), and a power output end of the cylinder (6) is connected to the piston.

4. A liposome extrusion device according to claim 1, characterized in that: The tank body (1) is also provided with a side passage pipe (13), one end of which is connected to the tank body (1) and the other end of which is connected to a water pump.

5. A liposome extrusion device according to claim 1, characterized in that: A sealing rubber ring (211) is sleeved on the outer periphery of the fixed portion (21), and the outer periphery of the sealing rubber ring (211) abuts against the inner peripheral wall of the rotating portion (22).

6. A liposome extrusion device according to claim 1, characterized in that: The rotating part (22) is threadedly connected to the tank body (1).

7. A liposome extrusion device according to claim 1, characterized in that: A flange (12) is axially arranged on the inner peripheral wall of the tank body (1), and the filter membrane (11) is arranged against the flange (12).

8. A liposome extrusion device according to claim 1, characterized in that: The partition plate (4) is provided with a plurality of through holes (42).

Images