Amplification module positioning and assembling structure and amplification device

Abstract

The utility model discloses an amplification module positioning assembly structure and an amplification device, and belongs to the technical field of medical instruments, the amplification module positioning assembly structure comprises a positioning part which is arranged on an amplification module and is close to one end part, and a fixing part which is spaced from the positioning part and is close to the other opposite end part of the amplification module; the assembly substrate is used for assembling the amplification module; an embedding part is arranged close to one end part of the assembly substrate; a clamping part is arranged at the other end part which is separated from the embedding part and is close to the assembling substrate; the positioning part is at least partially matched with the embedding part, and the fixing part is matched with the clamping part. The amplification module positioning and assembling structure can effectively solve the problems that in the prior art, due to the fact that parallel configuration of amplification modules is needed for batch processing of samples, the size and the mass of the amplification modules are large due to limitation of the characteristics of a current cycle amplification technology, the parallel configuration difficulty is high, and the like.

Description

Technical Field

[0001] The utility model belongs to the technical field of medical devices. Specifically, it relates to a positioning and assembly structure for an amplification module and an amplification device. Background Art

[0002] Polymerase chain reaction (PCR) is an important in vitro diagnostic technology. In the earlier period, the amplified product was detected by agarose gel electrophoresis to obtain qualitative or quantitative detection of the target gene. With the development of technology, real-time fluorescence PCR technology has gradually become a low-pollution and highly efficient mainstream qualitative or quantitative technology. It quantifies and analyzes the target fragment by using fluorescent-labeled probes, DNA intercalating agents, etc. to detect the exponential amplification process of the target gene in real time. Usually in various scenarios such as medical and biological research laboratories, for example, the detection of genetic diseases, the identification of genetic characteristics, the diagnosis of infectious diseases, gene cloning, paternity testing, and DNA computing. This method has been automated by using thermostable DNA polymerase and a machine that can quickly heat and cool samples (usually called an amplification module).

[0003] With the development of detection technology and the improvement of human living standards, more and more human or other biological samples need to be detected and processed. More manufacturers have designed detection systems with different sample processing throughputs to perform rapid batch detection. Various types of amplification modules based on variable temperature cycling technology have been researched and developed. German utility model DE202017106844U1 discloses a configuration scheme for an efficient thermal cycling module. CN208815024U discloses a scheme for configuring an electric heating unit on the side wall of the sample block to eliminate edge effects and increase overall thermal uniformity. Another utility model CN208815025U defines the layout difference in the emerging direction of the sample block fixing pressing block and the connecting wire of the thermoelectric heating unit. The scheme disclosed in US patent US20060065652A1 is also to configure an electric heating unit in the peripheral area below the thermoelectric heating unit, so that the heat of the thermoelectric unit and the electric heating unit can be more evenly transferred to the sample block through the heat transfer part. Chinese invention patent CN110975958A discloses an elastic mechanism for facilitating the ejection of the sample block and details the working mechanism of the ejection mechanism to ensure that the sample block can be quickly and conveniently replaced. All of the above designs are aimed at improving the thermal cycling characteristics of the amplification module or improving the sample block, etc. However, batch processing requires the parallel configuration of amplification modules. At present, due to the limitations of the characteristics of the cyclic amplification technology itself, the volume and mass of the module are relatively large, and the difficulty of parallel configuration is relatively high.

[0004] Therefore, designing a structure suitable for quickly assembling and fixing the amplification module is very important for the batch processing system, and it also plays a very important role in the maintenance and repair stage of the system. Summary of the Utility Model

[0005] The object of the present utility model is to provide an amplification module positioning and assembling structure and an amplification device for the above-mentioned deficiencies, so as to solve the problems in the prior art that due to the batch processing of samples, a parallel configuration of amplification modules is required, and due to the limitations of the characteristics of the current cycle amplification technology, the volume and mass of the amplification modules are relatively large, resulting in a relatively high difficulty in parallel configuration. To achieve the above object, the present utility model provides the following technical solutions:

[0006] An amplification module positioning and assembling structure includes a positioning portion disposed near one end of the amplification module, and a fixing portion spaced from the positioning portion and near the opposite end of the amplification module; it also includes an assembling substrate for assembling the amplification module; the assembling substrate is provided with a fitting portion near one end; a clamping portion is disposed at a position spaced from the fitting portion and near the opposite end of the assembling substrate; the amplification module can be pushed to move from one end of the assembling substrate provided with the clamping portion to the opposite end provided with the fitting portion, and when it moves to the end of the assembling substrate provided with the fitting portion, at least part of the positioning portion cooperates with the fitting portion, and the fixing portion cooperates with the clamping portion.

[0007] Further, the positioning portion includes a guiding unit; the fitting portion includes positioning posts, and when the amplification module moves to the end of the assembling substrate provided with the fitting portion, the guiding unit can be engaged and cooperate with the positioning posts.

[0008] Further, the guiding unit includes a V-shaped guiding groove; the positioning posts can be engaged and fitted into the bending groove bottom of the V-shaped guiding groove.

[0009] Further, the positioning portion further includes a roller group; the roller group includes at least two roller units arranged at intervals.

[0010] Further, the fitting portion includes at least two card slots corresponding to the number of roller units; when the amplification module moves to the end of the assembling substrate provided with the fitting portion, at least two of the roller units can be embedded and fitted into the corresponding card slots.

[0011] Further, the positioning portion further includes a clamping block; the fitting portion includes a fixed block with a semi-closed structure; when the amplification module moves to the end of the assembling substrate provided with the fitting portion, at least part of the clamping block is fitted into the fixed block.

[0012] Further, the clamping block includes a connecting section with a first thickness and a clamping section with a second thickness, where the first thickness is greater than the second thickness; the clamping section can be clamped into the fixed block with a semi-closed structure, and the number of the clamping blocks and the fixed blocks is the same and not less than two.

[0013] Further, a through hole is provided on the fixing portion; a connecting hole is provided on the engaging portion; when the amplification module moves to one end of the assembly substrate where the fitting portion is configured, the through hole and the connecting hole can be cooperatively connected through a connecting pin.

[0014] Further, the number of the through holes and the connecting holes is the same and not less than two.

[0015] An amplification device includes at least two assembly substrates arranged at intervals to form an amplification space capable of accommodating at least two amplification modules; in the amplification space, the assembly substrate and the corresponding amplification module are assembled in cooperation with the above-mentioned amplification module positioning and assembly structure.

[0016] The beneficial effects of the present utility model are as follows:

[0017] 1. The present utility model provides an amplification module positioning and assembly structure. By respectively configuring a positioning portion and a fixing portion at opposite end portions of the amplification module, and correspondingly arranging a fitting portion and an engaging portion on opposite sides of the corresponding assembly substrate, it is possible to push the amplification module to move from one end of the assembly substrate where the engaging portion is configured to the opposite end where the fitting portion is configured, and finally at least part of the positioning portion is matched with the fitting portion, and the fixing portion is matched with the engaging portion to achieve the goal of quickly positioning and fixing the amplification module. The design is simple and the operation is convenient. The positioning portion includes a V-shaped guiding unit, and the fitting portion includes a positioning post. When the amplification module moves to one end of the assembly substrate where the fitting portion is configured, the positioning post can be snap-fitted to the bottom of the bending groove of the V-shaped guiding groove, making the module assembly positioning simple and reliable.

[0018] 2. The present utility model provides an amplification module positioning and assembly structure. The positioning portion includes a roller group, which can enable the amplification module to be configured quickly and with low resistance during assembly or maintenance. The fitting portion includes a card slot corresponding to the number of rollers of the roller group. When the amplification module moves to one end of the assembly substrate where the fitting portion is configured, the roller unit can be embedded and fitted into the corresponding card slot, which also ensures the effect of reliable fixation without shaking of the amplification module after assembly in place.

[0019] 3. The present utility model provides an amplification module positioning and assembly structure. The positioning portion includes a clamping block, and the fitting portion includes a fixed block with a semi-closed structure. When the amplification module moves to one end of the assembly substrate where the fitting portion is configured, at least part of the clamping block is fitted into the fixed block, which can achieve more constraint fittings in addition to the cooperation between the positioning post and the V-shaped guiding unit, making the positioning constraint between the amplification module and the assembly substrate more reliable.

[0020] 4. The present utility model provides an amplification module positioning and assembly structure. The fixing portion and the engaging portion can be detachably connected by pins, screws or bolts, etc. The connection is reliable and convenient during assembly in place, and the disassembly is convenient when maintenance and repair are required. Multiple sets of configurations can also make the fixing reliability higher.

[0021] 5. The present utility model provides an amplification device, which includes at least two amplification modules, has a simple assembly and higher subsequent amplification efficiency, and supports parallel amplification processing. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the positioning part and the fixing part configured at opposite ends of the amplification module of the present utility model;

[0023] Figure 2 It is a schematic diagram of the fitting part and the engaging part configured on the assembly substrate of the present utility model;

[0024] Figure 3 It is a perspective view of one end of the amplification module of the present utility model placed on the assembly substrate where the engaging part is configured;

[0025] Figure 4 It is another perspective view of the amplification module of the present utility model placed on the assembly substrate where the engaging part is configured;

[0026] Figure 5 It is a perspective view of the amplification module of the present utility model after being pushed to move to the end where the fitting part is configured and fixed;

[0027] Figure 6 It is another perspective view of the amplification module of the present utility model after being pushed to move to the end where the fitting part is configured and fixed;

[0028] Figure 7 It is a schematic diagram of the structure of an amplification device provided by the present utility model;

[0029] In the drawings: 1 - clamping and transfer assembly, 2 - transmission channel, 3 - pipetting consumable box recycling part, 4 - amplification consumable recycling part, 5 - ventilation area, 6 - image acquisition unit, 10 - amplification module, 20 - clamping block, 201 - fixed block, 21 - roller group, 211 - card slot, 30 - guiding unit, 31 - positioning post, 40 - fixing part, 41 - connection hole. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0030] The following further details the present utility model in conjunction with the drawings and specific embodiments, but the present utility model is not limited to the following embodiments.

[0031] Embodiment 1:

[0032] See the attached Figures 1 to 6 . A positioning and assembly structure of an amplification module 10, such as Figures 1 - 6As shown, it includes a positioning part and a fixing part 40 arranged at two opposite ends of the amplification module 10. In this embodiment, the amplification module 10 is an amplification module 10 including a plurality of independent loading channels, so that it can adapt to the processing time schedule of the batch processing system and also adapt to the fluctuating changes in the sample processing volume of the batch processing system. The two opposite ends here can be configured as the front end and the rear end in the length direction of the amplification module 10. Such a setting can ensure that the assembled amplification module 10 is more convenient to add the object to be detected. A positioning part is arranged at the bottom of the front end of the amplification module 10. The positioning part includes a guiding unit 30. The guiding unit 30 includes a V-shaped guiding groove, which can be a detachable element and is assembled at the bottom front end of the amplification module 10 by screws or other detachable means. The opening end of the V-shaped guiding groove faces the front end of the amplification module 10. In this way, a greater mating opportunity with the elements in the fitting part of the assembly substrate is generated at the place with a larger opening degree. Even if the amplification module 10 is pushed and moves with a little deflection or tilt, the cooperation between the two can be reliably and efficiently achieved. Preferably, the opening expansion angle of the V-shaped guiding groove is an acute angle of 15°-75°. Of course, the structure of the guiding groove can also be a U-shaped or other groove types with a smaller change in the opening degree. The positioning part also includes a roller group 21. The roller group 21 includes at least two roller units arranged at intervals. Here, in order to ensure that the rollers can form a reliable and effective support for the amplification module 10, the optimal distance between the spaced roller units is 3 / 4-1.15 of the width of the amplification module 10. The configuration of the roller group 21 can make the amplification module 10 rollingly contact the assembly substrate. In this way, it has more convenient and operable characteristics for assembly and subsequent maintenance and repair. The positioning part also includes a clamping block 20. The clamping block 20 includes a connecting section with a first thickness and a clamping section with a second thickness, where the first thickness is greater than the second thickness. Preferably, the first thickness is 2-5 times the second thickness, so as to ensure that the clamping section is not too thick to affect the assembly and also ensure that the connecting section has sufficient strength to make its connection with the amplification module 10 more reliable. In this embodiment, the roller group 21 is fixedly connected to the clamping block 20, so that the number of connection points between the positioning part and the amplification module 10 is small and the reliability of the positioning part is higher. At a preset distance from the positioning part, a fixing part 40 is arranged at the bottom of the rear end of the amplification module 10. The optimal distance between the two here is 1 / 2-9 / 10 of the length dimension of the amplification module 10. In this way, the amplification module 10 can be more reliably fixed on the assembly substrate. The fixing part 40 is detachably connected to the bottom end of the amplification module 10 and can make at least part of it exposed outside the coverage of the amplification module 10. The fixing part 40 includes a plurality of through holes for connecting the amplification module 10 with connecting pieces.

[0033] The assembly substrate is as Figure 2As shown, it is configured to be a quadrilateral or other shape with a preset thickness, and can be made of metal materials such as steel and aluminum to ensure the strength of the structure. A fitting portion and a clamping portion are respectively configured at the front end and the rear end corresponding to the amplification module 10 of the assembly substrate. Here, the clamping portion includes a plurality of connection holes 41, and the connection holes 41 can be threaded holes or optical holes, which are not limited here. The fitting portion includes a fixed block 201 with a semi-closed structure, and also includes at least two card slots 211 corresponding to the number of roller units. Among them, the fixed block 201 is detachably installed on the assembly substrate, and the card slots 211 are configured as rectangular or other quadrilateral slots. A positioning post 31 is also configured at the position between the two fixed blocks 201.

[0034] When assembly is required, the operator places the amplification module 10 on the assembly substrate from the end of the assembly substrate where the clamping portion is configured. At this time, the state is as shown in Figure 3 and Figure 4 As shown, since the positioning portion on the amplification module 10 includes a roller group 21, during the assembly process, only the weight of part of the amplification module 10 needs to be supported and the amplification module 10 is pushed to make it roll on the assembly substrate. In this way, the assembly process is simple and requires less manpower. The amplification module 10 can be pushed to move from the end of the assembly substrate where the clamping portion is configured to the opposite end where the fitting portion is configured, and when it moves to the end of the assembly substrate where the fitting portion is configured, the positioning portion can at least partially cooperate with the fitting portion, and the fixing portion 40 cooperates with the clamping portion. Combining Figure 5 and Figure 6When the amplification module 10 moves to one end of the fitting part of the assembly substrate, the guiding unit 30 can be engaged and cooperated with the positioning post 31. Specifically, when the guiding unit 30 includes a V-shaped groove, the positioning post 31 can be engaged and cooperated with the bottom of the bending groove of the V-shaped guiding groove. In this way, the bending part of the V-shaped groove can also form a reliable restraint on the positioning post 31, so that the amplification module 10 will not have the risk of tilting or shaking even under the action of external force. In addition, when the amplification module 10 moves to one end of the fitting part of the assembly substrate, at least two roller units can be embedded and fitted into the corresponding card slots 211, and at least part of the clamping block 20 is fitted into the fixed block 201. Here, at least part of the clamping section with a smaller thickness of the clamping block 20 can be connected and fitted into the fixed block 201. In this way, a multiple restraint state of the cooperation between the positioning post 31 and the V-shaped guiding unit 30 and the connection and cooperation of at least part of the clamping block 20 and the fixed block 201 is formed. At the same time, at least part of the roller unit is embedded and fitted into the corresponding card slot 211, so that in the assembled normal use state, the roller units of the roller group 21 can bear less or even no gravity of the amplification module 10, and actually support the clamping block 20 and the fixed block 201, which also ensures the reliability of the roller unit. In special cases, such as when the amplification module 10 is tilted or distorted, this cooperation can also restrain the amplification module 10 to a certain extent. And the final fixation only needs to be based on the detachable fixed connection between the fixing part 40 and the engaging part. In one case, one or several connecting pins can be used to cooperatively connect the through hole and the connecting hole 41 to complete the fixation. The connection of the present application is simple, there are many restraint points, and the assembly tolerance is also large. And in the scenario that needs maintenance or repair, only the detachable fixed connection between the fixing part 40 and the engaging part needs to be removed, and then the amplification module 10 is pulled, and the module can be taken out with low resistance for maintenance and repair, and the maintenance convenience is also higher.

[0035] Embodiment 2:

[0036] See attachment Figures 1 to 7. An amplification device is constructed as a cubic structure using structural components as a whole and has a preset internal space. At the top of the front-end area of the device, a clamping and transfer assembly 1 is configured to move within a preset stroke range along the depth direction of the device. It includes a first transfer unit for transferring amplification consumables and a second transfer unit for transferring pipetting consumable cartridges. At the front bottom, an amplification consumable recycling section 4 and a pipetting consumable cartridge recycling section 3 are configured. In the middle area, a transmission channel 2 is configured, which can enable the amplification device to receive the amplification consumables with the system construction completed transferred from other modules, and can also transfer the used empty pipetting consumable cartridges. Under the action of the clamping and transfer assembly 1, different treatments can be performed on the transferred different consumables. At the innermost part in the depth direction of the amplification device, two assembly substrates are arranged at different positions. The arrangement of the two assembly substrates forms two amplification spaces in the amplification device that can accommodate two amplification modules 10. Of course, in some special scenarios, more assembly substrates can be configured at different heights to form an amplification device with higher processing capacity. Each assembly substrate can receive the amplification module 10 from the side with its configured clamping part and can be pushed to move towards the side where the fitting part is configured. Finally, each assembly substrate and the corresponding amplification mechanism can be assembled through the amplification module 10 positioning and assembly structure described above. In this way, the assembly of the amplification device is simple and efficient. The clamping and transfer assembly 1 can transfer the amplification consumables with the system construction completed transferred by the transmission channel 2 into the amplification module 10. The amplification module 10 can perform amplification after being fully loaded with amplification consumables or only partially loaded with amplification consumables, and obtain the detection results. The amplification consumables that have completed the detection are removed from the amplification module 10 by the clamping and transfer assembly 1 again and placed into the amplification consumable recycling section 4. In order to ensure that the amplification device has the characteristic of low pollution, a ventilation area 5 is also configured at the top of the device, which can be configured with a high-efficiency filtering component and a fan component to ensure that the air in the amplification device can be updated quickly, reducing the pollution risk. An image acquisition unit 6 is also configured at the top of the device, which can automatically and reliably acquire whether each functional unit is operating normally. The amplification device of the present application has a simple assembly, can automatically perform amplification and recycling processing, and the system including it has a higher degree of automation, is simple to manufacture, and has a lower cost.

[0037] The above are only the preferred embodiments of the present invention, and do not limit the patent scope of the present invention accordingly. Any equivalent structure or equivalent process transformation made by using the content of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, shall be equally included in the patent protection scope of the present invention.

Claims

1. An amplification module positioning assembly structure, characterized in that: The invention comprises a positioning portion arranged on an amplification module (10) near one end, and a fixing portion (40) spaced from the positioning portion and near the other end opposite to the amplification module (10); and also comprises an assembly substrate for assembling the amplification module (10); the assembly substrate is provided with a fitting portion near one end; and a snap-fit ​​portion is arranged spaced from the fitting portion and near the other end opposite to the assembly substrate; the amplification module (10) can be pushed to move from one end of the assembly substrate provided with the snap-fit ​​portion to the other end opposite to the fitting portion, and when moving to one end of the assembly substrate provided with the fitting portion, the positioning portion can at least partially cooperate with the fitting portion, and the fixing portion (40) can cooperate with the snap-fit ​​portion.

2. The amplification module positioning assembly structure according to claim 1, characterized in that: The positioning portion comprises a guide unit (30); the engaging portion comprises a positioning column (31); when the amplification module (10) moves to one end of the mounting substrate where the engaging portion is configured, the guide unit (30) can engage with the positioning column (31).

3. The amplification module positioning assembly structure according to claim 2, characterized in that: The guide unit (30) comprises a V-shaped guide groove; the positioning column (31) can be snap-fitted into the bending groove bottom of the V-shaped guide groove.

4. The amplification module positioning assembly structure according to claim 1, characterized in that: The positioning portion further comprises a roller group (21); the roller group (21) comprises at least two roller units arranged at intervals.

5. The amplification module positioning assembly structure according to claim 4, characterized in that: The engaging portion comprises at least two slots (211) corresponding to the number of roller units; when the amplification module (10) moves to one end of the mounting substrate where the engaging portion is configured, at least two of the roller units can be embedded and matched into the corresponding slots (211).

6. The amplification module positioning assembly structure according to claim 1, characterized in that: The positioning portion further comprises a card block (20); the engaging portion comprises a fixed block (201) of a semi-enclosed structure; when the amplification module (10) moves to one end of the mounting substrate configured with the engaging portion, the card block (20) is at least partially fitted into the fixed block (201).

7. The amplification module positioning assembly structure according to claim 6, characterized in that: The clamping block (20) comprises a connecting section of a first thickness and a clamping section of a second thickness, wherein the first thickness is greater than the second thickness; the clamping section can be clamped into a fixed block (201) of a semi-enclosed structure, and the number of the clamping block (20) and the fixed block (201) is the same, not less than two.

8. The amplification module positioning assembly structure according to claim 1, characterized in that: The fixing portion (40) is provided with a through hole; the engaging portion is provided with a connecting hole (41); when the amplification module (10) moves to one end of the mounting substrate configuration engaging portion, the through hole and the connecting hole (41) can be matched and connected via a connecting pin.

9. The amplification module positioning assembly structure according to claim 8, characterized in that: The through holes and the connecting holes (41) are the same in number and are no less than two.

10. An amplification device, characterized in that: It comprises at least two assembly substrates arranged at intervals to form an amplification space capable of accommodating at least two amplification modules (10); the assembly substrates and the corresponding amplification modules (10) in the amplification space are assembled in cooperation with the amplification module positioning assembly structure as claimed in any one of claims 1 to 9.

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