Micro-fluidic chip cell recovery device
By designing an integrated microfluidic chip cell recovery device, using moving parts and solenoid valve switches for lifting and lowering motion, the problem of lack of integration and inability to accurately control the cell recovery solution volume in the prior art is solved, and the effect of safe cell recovery and precise control of solution volume is achieved.
Patent Information
- Application Number
- CN202223137262.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2022-11-24
- Publication Date
- 2025-06-10
- Estimated Expiration
- 2032-11-24
AI Technical Summary
The existing microfluidic chip cell recovery devices lack integration, need to connect each cell recovery device separately, and cannot accurately control the cell recovery solution volume, especially during the vitrification and freezing process, there is a problem of insufficient volume.
A microfluidic chip cell recovery device is designed, including a device body, a scaffold and a moving component. The moving component can be lifted and moved, and is equipped with a detachable connecting connecting component. The solenoid valve switch drives the lifting and lowering movement of the moving parts, and the control device accurately controls the action of the solenoid valve switch, so as to achieve accurate control of the cell recovery solution volume.
The integration of cell recovery devices is realized, safe cell recovery is ensured, and the volume of cell recovery solution can be accurately controlled, and it is suitable for scenarios with small volume requirements during vitrification and freezing.
Smart Images
Figure CN222961431U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of cell processing, in particular to a cell recovery device for a microfluidic chip. Background Art
[0002] In a microfluidic chip involving cells, sometimes it is necessary to recover the processed cells, especially high-value cells. A relatively direct method is to directly discharge the cells into the cell recovery hole, and then use corresponding tools to complete the cell recovery. To ensure the safety of cell recovery, the cell recovery tool can also be placed into the cell recovery hole to directly recover the cells during the discharge process.
[0003] Defects of the background art are:
[0004] 1. Lack of integration, and each cell recovery device must be separately connected to the corresponding cell recovery hole.
[0005] 2. The volume of the solution for cell recovery cannot be controlled. Especially for the vitrification freezing process, it is required that the volume of cell recovery be small enough to ensure the subsequent freezing effect. Summary of the Utility Model
[0006] The utility model provides a cell recovery device for a microfluidic chip, which includes a device main body and a bracket. The bracket is installed on the device main body. The device main body is provided with a recovery hole. The bracket is provided with a moving part, and the moving part can perform a lifting movement on the bracket. The moving part is provided with a connecting part for detachably connecting with a cryopreservation tube.
[0007] As a further improvement of the utility model, the cell recovery device for the microfluidic chip further includes a driving mechanism for driving the moving part to perform a lifting movement on the bracket.
[0008] As a further improvement of the utility model, the driving mechanism includes a solenoid valve switch, and the moving part is connected to the valve core of the solenoid valve switch.
[0009] As a further improvement of the utility model, the cell recovery device for the microfluidic chip further includes a control device, and the control device can control the solenoid valve switch to perform a lifting action with a predetermined stroke within a predetermined time.
[0010] As a further improvement of the utility model, the cell recovery device for the microfluidic chip further includes a control device, and the control device can control the solenoid valve switch to perform a rising action with a predetermined stroke within a predetermined time.
[0011] As a further improvement of the utility model, the cell recovery device for the microfluidic chip further includes a control device, and the control device can control the solenoid valve switch to complete a 5-mm rising action within 0.1 s.
[0012] As a further improvement of the present utility model, the device body further has a chip interface, one end of the chip interface communicates with the recovery hole, and the other end of the chip interface is used to communicate with the cell recovery channel of the microfluidic chip.
[0013] As a further improvement of the present utility model, the moving part is a cantilever.
[0014] As a further improvement of the present utility model, the microfluidic chip cell recovery device further includes a first sealing ring, and the first sealing ring is installed at the connection between the other end of the chip interface and the cell recovery channel, so as to ensure the airtightness of the connection between the other end of the chip interface and the cell recovery channel.
[0015] As a further improvement of the present utility model, the microfluidic chip cell recovery device further includes a connection mechanism for connecting the device body and the microfluidic chip together.
[0016] As a further improvement of the present utility model, the device body is a clamp.
[0017] As a further improvement of the present utility model, the clamp includes an upper clamp body and a lower clamp body.
[0018] As a further improvement of the present utility model, the bracket is installed on the upper clamp body.
[0019] As a further improvement of the present utility model, the microfluidic chip cell recovery device further includes a communication structure for communicating the bracket and the clamp, and the communication structure includes a connection component for connecting the bracket and the clamp.
[0020] As a further improvement of the present utility model, the connection component is a plug-in part or a threaded connection part.
[0021] As a further improvement of the present utility model, the microfluidic chip cell recovery device further includes a cryopreservation tube, and the cryopreservation tube and the connection part are detachably connected.
[0022] As a further improvement of the present utility model, the connection part is an opening or a slot.
[0023] As a further improvement of the present utility model, the connection part is a through hole.
[0024] As a further improvement of the present utility model, the through hole is in a conical shape with a larger upper part and a smaller lower part.
[0025] As a further improvement of the present utility model, the through hole has a shape consistent with the outer shape of the cryopreservation tube.
[0026] The beneficial effects of the present utility model are as follows: By integrating the bracket, the recovery hole, and the clamp to form a cell recovery device, the present utility model can accurately control the volume of the cell recovery solution while ensuring the safe recovery of cells. Description of the Drawings
[0027] Figure 1 is a schematic structural diagram of the present utility model, in which the cryotube does not extend into the recovery hole;
[0028] Figure 2 is a schematic structural diagram of the present utility model, in which the cryotube has extended into the recovery hole. Detailed Embodiments
[0029] As Figure 1 、 2 shown, the present utility model discloses a cell recovery device, including a device main body and a bracket 207. The bracket 207 is installed on the device main body. The device main body is provided with a recovery hole 208. The bracket 207 is provided with a moving member, and the moving member can move up and down on the bracket 207. The moving member is provided with a connecting member for detachably connecting with a cryotube 211.
[0030] The connecting member provided on the moving member can be a hole, a groove, a clamping mechanism, etc., as long as the cryotube 211 can be fixed on the connecting member and can be removed from the connecting member. For example, the cryotube 211 is placed in the hole of the moving member, and the cryotube 211 is stuck in the hole. At the same time, the cryotube 211 can also be pulled out from the hole; similarly, the clamping mechanism can clamp the cryotube 211, and the cryotube 211 can also be taken out from the clamping mechanism. For example, the connecting member is an opening or a slot provided on the moving member, and the opening or the slot cooperates with the cryotube 211 to realize the fixing and placement of the cryotube 211. For example, the connecting member is a through hole provided on the moving member, and the through hole cooperates with the cryotube 211 to realize the fixing and placement of the cryotube 211. The through hole can be in a conical shape with a larger upper part and a smaller lower part, so as to cooperate with the cryotube 211 to realize the fixing and placement of the cryotube 211. The through hole can also have a shape consistent with the outer shape of the cryotube 211 for adapting to the cryotube 211, so as to better fix and place the cryotube 211.
[0031] The moving member can move up and down on the bracket 207 can be realized by a mechanical structure and a manual method. As a preferred embodiment of the present utility model, the microfluidic chip cell recovery device further includes a driving mechanism for driving the moving member to move up and down on the bracket 207.
[0032] In some embodiments, the driving mechanism includes a solenoid valve switch, the moving part is a cantilever 212, the cantilever 212 is connected to the valve core of the solenoid valve switch, and the valve core of the solenoid valve switch drives the cantilever 212 to lift. A cryotube 211 is placed on the connecting part of the cantilever 212. The lifting of the cantilever 212 drives the cryotube 211 to rise, so that the cryotube 211 is separated from the cell recovery channel.
[0033] The cell recovery device of the microfluidic chip further includes a control device, which can control the solenoid valve switch. As its first embodiment: the control device can control the solenoid valve switch to perform a lifting action with a predetermined stroke within a predetermined time; as its second embodiment: the control device can control the solenoid valve switch to perform a rising action with a predetermined stroke within a predetermined time. For example, the control device can control the solenoid valve switch to complete a 5-mm rising action within 0.1 s.
[0034] In some embodiments, the device body further has a chip interface. One end of the chip interface is communicated with the recovery hole 208, and the other end of the chip interface is used to communicate with the cell recovery channel of the microfluidic chip.
[0035] In a further embodiment, the cell recovery device further includes a first sealing ring, and the first sealing ring is installed at the connection between the other end of the chip interface and the cell recovery channel, so as to ensure the airtightness of the connection between the other end of the chip interface and the cell recovery channel.
[0036] The cell recovery device further includes a connection mechanism for connecting the device body and the microfluidic chip together, so as to reasonably fix and connect the microfluidic chip and the cell recovery device, and facilitate the transfer process of cells from the microfluidic chip to the cell recovery device and other components.
[0037] As a preferred embodiment of the present invention, the device body is a fixture, and the microfluidic chip is fixed by the fixture to realize the position fixing and connection of the microfluidic chip.
[0038] The fixture includes an upper clamp body 203 and a lower clamp body 205. A bracket 207 is installed on the upper clamp body 203, and the recovery hole 208 is also provided at the position of the upper clamp body 203.
[0039] In a further embodiment, it further includes a communication structure for communicating the bracket 207 and the fixture. In a further embodiment, the communication structure includes a connection component, and the connection component is used to connect the bracket 207 and the fixture to realize the connection and fixation of the bracket 207 and the fixture.
[0040] In a further embodiment, the connecting component can be a mechanical connection method such as a plug-in or a threaded connection. In the actual use scenario, a threaded connection can be preferably used, which is convenient for connection.
[0041] During operation, the microfluidic chip is placed in the fixture and fixed tightly by the fixture; the cryotube 211 is installed on the connecting component of the cantilever 212, and the cryotube 211 extends into the recovery hole 208. In the non-powered state, the valve core of the solenoid valve switch is in a lower position, and the end of the cryotube 211 is communicated with the cell recovery channel of the microfluidic chip; due to capillary action, cells and solution enter the cryotube 211. When the solenoid valve switch is powered on, the valve core drives the cantilever 212 to lift, so that the cryotube 211 is separated from the cell recovery channel and no longer sucks the solution. By controlling the timing of the lifting of the cantilever 212, the volume of the cell recovery solution can be controlled; at this time, the cells are located in the cryotube 211 and are wrapped by a specified small amount of solution; then the cryotube 211 is taken out.
[0042] The utility model can realize the rapid assembly of the microfluidic chip and the cell recovery device and the accurate and micro-volume cell recovery.
[0043] The utility model integrates the bracket 207 and the recovery hole 208 with the fixture to form a cell recovery device, which can not only ensure the safe recovery of cells but also accurately control the volume of the cell recovery solution. The utility model modularizes the chip material transmission, which can not only connect all the transmission functional units with the microfluidic chip at one time but also has high flexibility, can be customized according to the structure of the microfluidic chip, and has both integration and flexibility.
[0044] The above content is a further detailed description of the utility model in combination with specific preferred embodiments. It cannot be determined that the specific implementation of the utility model is only limited to these descriptions. For those of ordinary skill in the technical field to which the utility model belongs, without departing from the concept of the utility model, several simple deductions or substitutions can be made, which should all be regarded as belonging to the protection scope of the utility model.
Claims
1. A microfluidic chip cell recovery device, characterized in that: it includes a device main body and a bracket (207), and the bracket (207) is installed on the device main body, the device main body is provided with a recovery hole (208), the bracket (207) is provided with a moving part, and the moving part can perform a lifting movement on the bracket (207), the moving part is provided with a connecting part for detachably connecting with a cryotube (211).
2. The microfluidic chip cell recovery device according to claim 1, characterized in that: the microfluidic chip cell recovery device further includes a driving mechanism for driving the moving part to perform a lifting movement on the bracket (207).
3. The microfluidic chip cell recovery device according to claim 2, characterized in that: the driving mechanism includes a solenoid valve switch, and the moving part is connected to the valve core of the solenoid valve switch.
4. The microfluidic chip cell recovery device according to claim 3, characterized in that: the microfluidic chip cell recovery device further includes a control device, and the control device can control the solenoid valve switch to perform a lifting action with a predetermined stroke within a predetermined time.
5. The microfluidic chip cell recovery device according to claim 3, characterized in that: the microfluidic chip cell recovery device further includes a control device, and the control device can control the solenoid valve switch to perform a rising action with a predetermined stroke within a predetermined time.
6. The microfluidic chip cell recovery device according to claim 5, characterized in that: the microfluidic chip cell recovery device further includes a control device, and the control device can control the solenoid valve switch to complete a 5-mm rising action within 0.1 s.
7. The microfluidic chip cell recovery device according to claim 1, characterized in that: the device main body also has a chip interface, one end of the chip interface is communicated with the recovery hole (208), and the other end of the chip interface is used for communicating with the cell recovery channel of the microfluidic chip.
8. The microfluidic chip cell recovery device according to claim 1, characterized in that: the moving part is a cantilever (212).
9. The microfluidic chip cell recovery device according to claim 7, characterized in that: the microfluidic chip cell recovery device further includes a first sealing ring, and the first sealing ring is installed at the connection between the other end of the chip interface and the cell recovery channel, so as to ensure the airtightness of the connection between the other end of the chip interface and the cell recovery channel.
10. The microfluidic chip cell recovery device according to any one of claims 1-9, characterized in that: the microfluidic chip cell recovery device further includes a connecting mechanism for connecting the device main body and the microfluidic chip together.
11. The microfluidic chip cell recovery device according to any one of claims 1-9, characterized in that: the device main body is a clamp.
12. The microfluidic chip cell recovery device according to claim 11, characterized in that: the clamp includes an upper clamp body (203) and a lower clamp body (205).
13. The microfluidic chip cell recovery device according to claim 12, characterized in that: The bracket (207) is installed on the upper clamp body (203).
14. The microfluidic chip cell recovery device according to claim 11, wherein: The microfluidic chip cell recovery device further includes a communication structure for communicating the bracket (207) with the clamp. The communication structure includes a connection component for connecting the bracket (207) and the clamp.
15. The microfluidic chip cell recovery device according to claim 14, wherein: The connection component is a plug-in component or a threaded connection component.
16. The microfluidic chip cell recovery device according to claim 1, wherein: The microfluidic chip cell recovery device further includes a cryotube (211), and the cryotube (211) is detachably connected to the connection component.
17. The microfluidic chip cell recovery device according to claim 1, wherein: The connection component is an opening or a slot.
18. The microfluidic chip cell recovery device according to claim 1, wherein: The connection component is a through hole.
19. The microfluidic chip cell recovery device according to claim 18, wherein: The through hole is in a tapered shape with a larger upper part and a smaller lower part.
20. The microfluidic chip cell recovery device according to claim 18, wherein: The through hole has a shape consistent with the outer shape of the cryotube (211).