Inoculating and planting integrated cup special for liquid strain
By designing a integrated cup for special inoculation and planting for liquid bacteria, the problem of separation of inoculation and mycelium cultivation in edible fungi cultivation is solved, and the complete process of inoculation, mycelium cultivation and fruiting body cultivation is achieved, and a comprehensive study of edible fungi cultivation methods is promoted.
Patent Information
- Application Number
- CN202421633091.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-11
- Publication Date
- 2025-06-13
- Estimated Expiration
- 2034-07-11
AI Technical Summary
In the prior art, the inoculation and mycelium cultivation links of edible fungi cultivation are separated, which limits the comprehensive study of edible fungi cultivation methods by the testers.
A liquid bacterial strain-specific inoculation and implantation integrated cup is designed, which includes a cup body, a cup lid and an inoculation tube, which can complete bacterial inoculation, mycelium culture and fruiting body cultivation in the integrated cup.
The complete process of inoculation, mycelium culture and fruiting body cultivation is achieved, which facilitates the experimenter to conduct more comprehensive research and understanding of edible fungi cultivation methods.
Smart Images

Figure CN222967573U_ABST
Abstract
Description
Technical Field
[0001] The utility model belongs to the technical field of edible mushroom cultivation, and particularly relates to a special inoculation and planting integrated cup for liquid spawns. Background Art
[0002] Edible mushrooms, also known as edible fungi, in a broad sense refer to all edible fungi. The edible mushrooms we usually refer to are those in a narrow sense, mainly including Pleurotus ostreatus, Lentinula edodes, Tremella fuciformis, Auricularia auricula-judae, Volvariella volvacea, Coprinus comatus, Pleurotus eryngii, Pleurotus nebrodensis, Agaricus blazei, Boletus edulis, Agaricus bisporus, Flammulina velutipes, Pholiota nameko, etc., which are also called edible mushrooms or edible toadstools, and commonly known as mushrooms. Edible mushrooms are high-protein, low-fat, and rich in vitamins, minerals, and dietary fiber, making them delicious and nutritious foods.
[0003] When cultivating edible mushrooms in a narrow sense, the spawns are usually inoculated into a spawn bag or a cultivation vessel with an inoculation device first, and then placed in a mushroom house for cultivation. Since the inoculation device and the mushroom house are separate, when conducting edible mushroom cultivation research in a laboratory, it is inconvenient for the experimenters to study the inoculation and mycelium cultivation links, and the research is restricted to a certain extent. Therefore, it is very necessary to study an integrated device for edible mushroom inoculation and cultivation. Content of the Utility Model
[0004] The purpose of the utility model is to provide a special inoculation and planting integrated cup for liquid spawns, which can complete the work of spawn inoculation, mycelium cultivation, and fruiting body cultivation in the integrated cup, and is conducive to a more comprehensive study of the edible mushroom cultivation method.
[0005] To achieve the above purpose, the utility model adopts the following technical solutions:
[0006] A special inoculation and planting integrated cup for liquid spawns, comprising:
[0007] A cup body, which contains a cultivation substrate inside;
[0008] A cup cover, which is detachably connected to the top of the cup body and is provided with at least two cup cover through holes, and one of the cup cover through holes is arranged at the center of the top of the cup cover;
[0009] An inoculation tube, the lower part of which extends into the cultivation substrate, is provided with a plurality of inoculation tube through holes, and the upper part is arranged inside the cup cover, and the top end is close to the cup cover through hole at the center of the top of the cup cover.
[0010] Further, the inoculation tube includes an inoculation main tube and inoculation branch tubes. The inoculation main tube is arranged vertically, and there are multiple inoculation branch tubes. The top ends of the inoculation branch tubes are communicated with the middle part and / or the lower part of the inoculation main tube, and are arranged in a divergent shape around the inoculation main tube. The parts corresponding to the inside of the cultivation substrate are provided with a plurality of inoculation tube through holes.
[0011] Furthermore, the bottom end of the inoculation branch tube is pointed, or is connected to an arrow-shaped branch tube plug.
[0012] Furthermore, it also includes an inoculation head, which is funnel-shaped, and the lower part can be detachably passed through the cup cover through hole at the top center of the cup cover to match and insert into the top of the inoculation tube, and the upper part is arranged outside the cup cover.
[0013] Furthermore, the portion of the inoculation tube extending into the cultivation substrate is made of a hard, high-temperature resistant plastic material, and the portion of the inoculation head exposed above the cup cover is made of an elastic, soft, high-temperature resistant plastic or silicone.
[0014] Furthermore, the cup body includes a cup body and a water storage mechanism, the water storage mechanism is detachably connected to the lower part of the cup body, a water leakage plate is provided at the bottom of the cup body, the water leakage plate is pierced with a plurality of water holes, and a water-permeable filter membrane is laid on the top or bottom surface.
[0015] Furthermore, the cup body is provided with a plurality of cup through holes.
[0016] Furthermore, at least one overflow hole is formed in the upper portion of the water storage mechanism.
[0017] Furthermore, the water leakage plate is detachably connected to the cup body.
[0018] Furthermore, the water leakage plate is matched and attached to the inner wall of the cup body, and a handle is provided on the bottom surface;
[0019] The inner wall of the cup body is provided with at least two support bar grooves, into which the support bar can be inserted; the support bar protrudes from the inner wall of the cup body, and can support the water leakage plate.
[0020] When assembling the above-mentioned inoculation and planting integrated cup for liquid strains, first put the cultivation matrix into the cup body, then punch a hole in the cultivation matrix, insert the inoculation tube, and cover the cup cover. During inoculation, the liquid strain is introduced into the inoculation tube through the through hole of the cup cover at the top center of the cup cover. After the inoculation is completed, the through hole of the cup cover can be blocked with a sponge plug to prevent foreign bacteria, small organisms, etc. from entering the cup body and contaminating the strains. When the mycelium culture is completed and the edible fungi are grown, the cup cover can be completely opened according to actual needs, or only the sponge plug can be taken out to provide a ventilated environment for the growth of edible fungi.
[0021] The utility model has the following advantages:
[0022] (1) By using the special inoculation and planting integrated cup for liquid spawn of the present utility model, the processes of inoculation, mycelium cultivation, and fruiting body cultivation can be completed. In this way, experimenters can test the spawn cultivation conditions before inoculation. After the experiment, the present utility model can be used to test the influence of spawn cultivation conditions on the cultivation of edible fungi. At the same time, it can also test the influence of different conditions in the inoculation process, mycelium cultivation process, and fruiting body growth process on the cultivation of edible fungi, facilitating experimenters to conduct more comprehensive research on the cultivation of edible fungi.
[0023] (2) The present utility model can also be used for the cultivation of edible fungi at home, enabling people to more comprehensively understand the whole process of inoculation, mycelium cultivation, and fruiting body growth in the cultivation of edible fungi. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] Figure 1 is a perspective structural schematic diagram of an embodiment of the present utility model.
[0025] Figure 2 is a three-dimensional structural schematic diagram of the inoculation tube.
[0026] Figure 3 is a three-dimensional structural schematic diagram of the cup body and the cup lid.
[0027] Figure 4 is a three-dimensional structural schematic diagram of the cup body main body.
[0028] Figure 5 is a three-dimensional structural schematic diagram of the water storage mechanism.
[0029] The reference numerals in the drawings are as follows:
[0030] Cup lid 100, cup lid through hole 110, cup body 200, water storage mechanism 210, overflow hole 211, cup body main body 220, cup body through hole 221, water leakage plate 222, handle 223, support bar 224, cup handle 230, inoculation tube 300, inoculation main tube 310, inoculation branch tube 320, branch tube plug 321, inoculation tube through hole 320, inoculation head 400. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0031] In order to make the objectives, technical solutions, and advantages of the present utility model clearer, the present utility model will be further described in detail below with reference to the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present utility model and are not used to limit the present utility model.
[0032] In the description of the present utility model, it should be noted that if the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside", etc. appear, the orientation or position relationship indicated is based on the orientation or position relationship shown in the accompanying drawings, or is the orientation or position relationship in which the utility model product is usually placed when used. It is only for the convenience of describing the utility model and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present utility model.
[0033] like Figures 1-5 As shown, a liquid fungus inoculation and planting integrated cup includes a cup body 200, a cup cover 100 and an inoculation tube 300, wherein: the cup body 200 contains a cultivation substrate. The cup cover 100 is detachably connected to the top of the cup body 200, and is provided with at least two cup cover through holes 110, wherein one cup cover through hole 110 is provided at the top center of the cup cover 100. The cup cover 100 and the cup body 200 generally adopt a connection method with good sealing performance, such as threaded connection, or a sealing ring is provided on the outer wall of the top of the cup body 200 or the inner wall of the bottom of the cup cover 100. When the cup cover 100 is covered on the top of the cup body 200, the contact position can be well sealed, so as to better prevent the fungus from being contaminated during the mycelium culture; the cup cover 100 is preferably made of transparent material to facilitate the observation of the growth of edible fungi, and when the edible fungi grow, light can still be obtained when the cup cover 100 is not opened. The inoculation tube 300, whose lower part extends into the cultivation matrix, is provided with a plurality of inoculation tube through holes 320, and whose upper part is arranged in the cup cover 100, and the top end is close to the cup cover through hole 110 at the top center of the cup cover 100, and the liquid bacteria can enter the cultivation matrix through the inoculation tube 300, and the plurality of inoculation tube through holes 320 can enable the liquid bacteria to enter different parts of the cultivation matrix. During assembly, the cultivation matrix is first loaded into the cup body 200, and then a hole is punched in the cultivation matrix, the inoculation tube 300 is inserted, and the cup cover 100 is covered. During inoculation, the liquid bacteria are introduced into the inoculation tube 300 through the cup cover through hole 110 at the top center of the cup cover 100, and the introduction method can be a dropper or the like; the other cup cover through hole 110 is to maintain the balance of air pressure inside and outside the cup cover 100, to prevent the liquid bacteria from squeezing out the gas in the cultivation matrix, so that the air pressure inside the cup cover 100 increases and causes the cup cover 100 to fall off. After the inoculation is completed, the through hole 110 of the cup cover can be sealed with a sponge plug or a plastic plug to prevent foreign bacteria, small organisms, etc. from entering the cup body 200 and contaminating the fungus. When the mycelium culture is completed and edible fungi are grown, the cup cover 100 can be completely opened according to actual needs, or only the sponge plug or the plastic plug can be removed to provide a ventilated environment for the growth of edible fungi.
[0034] Further, combined with Figure 2As shown in the figure, this embodiment provides a preferred inoculation tube 300. The inoculation tube 300 includes an inoculation main tube 310 and inoculation branch tubes 320. The inoculation main tube 310 is vertically arranged, and the bottom end is preferably closed, or the aperture of the bottom end is less than 0.2 cm. There are multiple inoculation branch tubes 320. The top ends are communicated with the middle part and / or the lower part of the inoculation main tube 310, and are arranged in a divergent shape on the peripheral side of the inoculation main tube 310. A plurality of inoculation tube through holes 320 are provided in the corresponding part in the cultivation substrate. The liquid spawn first enters the inoculation main tube 310, and then is diverted from the inoculation main tube 310 to the inoculation branch tubes 320. The spawn enters the cultivation substrate from the inoculation main tube 310 and the inoculation branch tubes 320. For the number of the inoculation branch tubes 320, as shown in the figure, there are 4 inoculation branch tubes 320, and one inoculation branch tube 320 is respectively arranged in the front, rear, left and right directions of the inoculation main tube 310. It can also be that two inoculation branch tubes 320 are respectively arranged in the front, rear, left and right directions at different height positions of the inoculation main tube 310, a total of eight. The inoculation branch tubes 320 at relatively high positions extend and expand towards the upper peripheral side of the cultivation substrate, and the inoculation branch tubes 320 at relatively low positions extend and expand towards the lower peripheral side of the cultivation substrate. In this way, the liquid spawn can be better distributed at various positions of the cultivation substrate. Of course, according to needs, other appropriate numbers of inoculation branch tubes 320 can be selected and set.
[0035] Further, the bottom end of the inoculation branch tube 320 is in a pointed shape, or is connected with an arrow-shaped branch tube plug 321. As Figure 2 shown in the figure, an arrow-shaped branch tube plug 321 is inserted and matched at the bottom end of the inoculation branch tube 320. During assembly, a hole is drilled in the cultivation substrate, generally a vertical hole is drilled, which can be matched and inserted with the inoculation main tube 310. And by making the bottom end of the inoculation branch tube 320 in a pointed design, it will be better inserted into the inoculation branch tube 320.
[0036] Further, to facilitate the introduction of liquid spawn, this embodiment further includes an inoculation head 400. The inoculation head 400 is in a funnel shape. The lower part can be detachably inserted through the cup lid through hole 110 at the center of the top of the cup lid 100 and matched and inserted into the top end of the inoculation tube 300. The upper part is arranged outside the cup lid 100. Specifically, the lower part of the inoculation head 400 is a tubular structure, which can be matched and inserted into the top end of the inoculation tube 300, and the upper part is a funnel-shaped structure, which is arranged outside the cup lid 100 to facilitate the introduction of liquid spawn. It is inserted into the top end of the inoculation tube 300 during inoculation and can be taken out after inoculation is completed.
[0037] Furthermore, the part of the inoculation tube 300 extending into the cultivation substrate is made of a rigid high-temperature resistant plastic material, such as PP, PES, PPSU, etc. The part of the inoculation head 400 protruding above the cup cover 100 is made of an elastic soft high-temperature resistant plastic or silica gel. The soft high-temperature resistant plastic such as TPE. By blowing air downward by squeezing the upper part of the inoculation head 400, the liquid containing bacteria with a higher concentration can be blown into the cultivation substrate. Further, this embodiment provides a preferred cup body 200, in combination with Figures 3-5 As shown, the cup body 200 includes a cup body main body 220 and a water storage mechanism 210. The water storage mechanism 210 is detachably connected to the lower part of the cup body main body 220. A water leakage plate 222 is provided at the bottom of the cup body main body 220. The water leakage plate 222 is provided with a plurality of water falling holes. A water-permeable filter membrane is laid on the top surface or the bottom surface. The pore diameter of the filter membrane is preferably 50-100 μm, which can permeate water under normal pressure conditions, but the water permeation speed is slowed down, and at the same time, the cultivation substrate is prevented from falling. When watering or fertilizing the edible fungi, the excess water can enter the water storage mechanism 210 through the water falling holes, keeping the moisture in the cultivation substrate appropriate, and reducing the probability that the mycelium or the fruiting body will die due to lack of oxygen. The water storage mechanism 210 is provided to prevent water from flowing out and polluting the environment.
[0038] Furthermore, a plurality of cup body through holes 221 are provided in the cup body main body 220. When too much water is poured into the cultivation substrate, the evaporation of the water in the cultivation substrate can be accelerated through the cup body through holes 221, and at the same time, a certain amount of oxygen can be provided for the growth of the mycelium or the fruiting body. During inoculation and mycelium cultivation, the cup body through holes 221 can be blocked with a sponge plug or a plastic plug. During the growth of the edible fungi, part or all of the sponge plug or the plastic plug can be taken out as needed.
[0039] Furthermore, at least one overflow hole 211 is provided in the upper part of the water storage mechanism 210. If there is more than one overflow hole 211, the overflow holes 211 are arranged at the same position in the upper part of the water storage mechanism 210. To prevent the water stored in the water storage mechanism 210 from being too much and soaking the cultivation substrate and affecting the growth of the mycelium or the fruiting body. During inoculation and mycelium cultivation, the overflow hole 211 also needs to be blocked with a sponge plug or a plastic plug.
[0040] Furthermore, this embodiment provides a preferred installation method for the water leakage plate 222. The water leakage plate 222 is detachably connected to the cup body 220, and there are various ways of detachable connection. For example, the water leakage plate 222 is fitted to the inner wall of the cup body 220, and a handle 223 is provided on the bottom surface; at least two support bar grooves are provided on the inner wall of the cup body 220, which can be inserted into the support bars 224 in a matching manner. The support bars 224 protrude from the inner wall of the cup body 220 and can support the water leakage plate 222 in a matching manner. When installing the water leakage plate 222, first place the water leakage plate 222 at the bottom of the cup body 220, and then insert the support bars 224 into the support bar grooves below the water leakage plate 222. After the installation of the support bars 224 is completed, hold the handle 223 and adjust the water leakage plate 222 so that it is horizontally placed on the support bars 224. If the water leakage plate 222 is damaged, the water leakage plate 222 can be replaced and still be used. It should be noted that the cup body 220 and the water storage mechanism 210 are generally connected by threads. Therefore, the inner wall of the bottom of the cup body 220 is provided with internal threads, and the outer wall of the top of the water storage mechanism 210 is provided with external threads to avoid affecting the installation of the water leakage plate 22.
[0041] A cup handle 230 can also be provided on the outer wall of the cup body 220 to facilitate the transfer of the position.
[0042] Using the special inoculation and planting integrated cup for liquid spawn of this embodiment, the links of inoculation, mycelium cultivation, and fruiting body cultivation can be completed. In this way, the experimenter can test the spawn cultivation conditions before inoculation. After the experiment is completed, the special inoculation and planting integrated cup for liquid spawn of this embodiment can be used to test the influence of spawn cultivation conditions on the cultivation of edible fungi. At the same time, the influence of different conditions in the inoculation link, mycelium cultivation link, and fruiting body growth link on the cultivation of edible fungi can also be tested, which is convenient for the experimenter to conduct a more comprehensive study on the cultivation of edible fungi.
[0043] All components of this embodiment, including the cup body 200, the cup lid 100, and the inoculation tube 300, except for the filter membrane laid on the top or bottom surface of the water leakage plate 222, are preferably made of high-temperature resistant materials, such as plastics that can withstand a high temperature of 121°C. In this way, each component of the integrated cup can be placed in an autoclave for disinfection to kill bacteria, viruses, etc., and improve the success rate of inoculation.
[0044] In addition, the special inoculation and planting integrated cup for liquid spawn of this embodiment can also be used for the cultivation of edible fungi at home, enabling people to have a more comprehensive understanding of the whole process of inoculation, mycelium cultivation, and fruiting body growth in the cultivation of edible fungi.
[0045] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims
1. A liquid strain inoculation and planting integrated cup, characterized in that include: The cup body contains a cultivation substrate; A cup cover, which is detachably connected to the top of the cup body and is provided with at least two cup cover through holes, one of which is provided at the top center of the cup cover; The inoculation tube has a lower portion extending into the cultivation substrate and is provided with a plurality of inoculation tube through holes, and an upper portion arranged in the cup cover, with the top end close to the cup cover through hole at the top center of the cup cover.
2. The liquid fungus inoculation and planting integrated cup according to claim 1, characterized in that: The inoculation tube comprises an inoculation main tube and an inoculation branch tube. The inoculation main tube is vertically arranged, and a plurality of inoculation branch tubes are provided. The top end is connected with the middle and / or lower part of the inoculation main tube, and the branch tubes are arranged in a divergent shape around the inoculation main tube. A plurality of inoculation tube through holes are pierced corresponding to the part in the cultivation matrix.
3. The liquid strain inoculation and planting integrated cup according to claim 2, characterized in that: The bottom end of the inoculation branch tube is pointed, or is connected with an arrow-shaped branch tube plug.
4. The liquid fungus inoculation and planting integrated cup according to claim 1, characterized in that: It also includes an inoculation head, which is funnel-shaped, and the lower part can be detachably passed through the cup cover through hole at the top center of the cup cover to match and insert into the top of the inoculation tube, and the upper part is arranged outside the cup cover.
5. The liquid fungus inoculation and planting integrated cup according to claim 4, characterized in that: The portion of the inoculation tube extending into the cultivation substrate is made of a hard high-temperature resistant plastic material, and the portion of the inoculation head exposed above the cup cover is made of an elastic soft high-temperature resistant plastic or silicone.
6. The liquid fungus inoculation and planting integrated cup according to claim 1, characterized in that: The cup body comprises a cup body and a water storage mechanism, wherein the water storage mechanism is detachably connected to the lower part of the cup body, a water leakage plate is arranged at the bottom of the cup body, a plurality of water holes are penetrated in the water leakage plate, and a water-permeable filter membrane is laid on the top or bottom surface.
7. The liquid fungus inoculation and planting integrated cup according to claim 6, characterized in that: The cup body is provided with a plurality of cup through holes.
8. The liquid fungus inoculation and planting integrated cup according to claim 6, characterized in that: At least one overflow hole is formed on the upper portion of the water storage mechanism.
9. The liquid strain inoculation and planting integrated cup according to any one of claims 6 to 8, characterized in that: The water leakage plate is detachably connected to the cup body.
10. The liquid fungus inoculation and planting integrated cup according to claim 9, characterized in that: The water leakage plate is matched and attached to the inner wall of the cup body, and a handle is provided on the bottom surface; The inner wall of the cup body is provided with at least two support bar grooves, into which the support bar can be inserted; the support bar protrudes from the inner wall of the cup body, and can support the water leakage plate.