Reagent bottle for lysate
By designing an automatic mixing reagent bottle, the problem that existing reagent bottles cannot achieve the separation and storage of cell lysate and PMSF is solved, and automated mixing is achieved, reducing operational complexity and human errors.
Patent Information
- Application Number
- CN202422092044.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-28
- Publication Date
- 2025-06-13
- Estimated Expiration
- 2034-08-28
AI Technical Summary
The existing reagent bottle design cannot achieve the separation and storage of cell lysate and PMSF. The operator must manually add PMSF before use, which increases the operating steps and complexity and is prone to human errors.
A reagent bottle consisting of an upper storage tank component set and a lower storage tank component set is designed, and automatic mixing of PMSF and lysate is achieved through the connection assembly, eliminating the step of manual addition.
It realizes easy mixing of PMSF and lysate before use, reducing operation steps and complexity, avoiding human errors, and improving operation accuracy and efficiency.
Smart Images
Figure CN222974045U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of reagent bottles, and specifically relates to a reagent bottle for lysate. Background Art
[0002] Cell lysate is a kind of rapid cell tissue lysate that can be used to extract proteins. Before using the cell lysate, PMSF (a protease inhibitor) needs to be added to the lysate. Generally, within a few minutes before use, PMSF is added to the lysate to keep the performance of the cell lysate in a better state. Staff usually use a sampling device to add PMSF into the reagent bottle containing the lysate. The current reagent bottle design cannot achieve the separated storage of cell lysate and PMSF. Operators can only manually add PMSF before use, which increases the operation steps and complexity, and may cause human errors during the addition, such as inaccurate dosage. Content of the Utility Model
[0003] In order to overcome the above technical problems, the purpose of the utility model is to provide a reagent bottle for lysate, so as to solve the problem that the current reagent bottle design cannot achieve the separated storage of cell lysate and PMSF, and operators can only manually add PMSF before use, which increases the operation steps and complexity as mentioned in the above background art.
[0004] To achieve the above purpose, the utility model provides the following technical solutions:
[0005] A reagent bottle for lysate, comprising: an upper storage tank component group for storing PMSF, the upper storage tank component group includes an upper tank body and an upper tank cover; a lower storage tank component group is arranged at the bottom of the upper storage tank component group for storing lysate, the lower storage tank component group includes a lower tank body, a lower tank cover and a lower tank seat; a connection component is arranged between the upper storage tank component group and the lower storage tank component group for connecting the upper storage tank component group and the lower storage tank component group, the connection component includes a vertical cylinder, a first spring piece is connected inside the vertical cylinder, one end of the first spring piece is connected with a sealing ball, a thimble piece is arranged at the bottom of the sealing ball, a second spring piece is arranged outside the vertical cylinder, and a first limiting half ring and a second limiting half ring are arranged outside the second spring piece.
[0006] Preferably: the upper tank cover is connected to the bottle mouth of the upper tank body by threads, the bottle mouth of the lower tank body is at the bottom, and the bottle mouth of the lower tank body is connected to the lower tank cover by threads, and the lower tank seat is connected to the bottom of the lower tank body.
[0007] Preferably, the vertical cylinder is fixedly connected to the bottom of the upper tank body through a through hole. The first spring member is connected between the vertical cylinder and the sealing ball. An opening is formed at the bottom of the vertical cylinder, and the bottom of the sealing ball contacts the opening of the vertical cylinder. A groove is provided on the side surface of the vertical cylinder, and the groove on the side surface of the vertical cylinder is used to communicate the inside of the vertical cylinder with the upper tank body.
[0008] Preferably, the thimble member is connected to the top of the lower tank body through a bracket. An opening is provided at the position of the lower tank body corresponding to the vertical cylinder, and the vertical cylinder contacts the opening of the lower tank body.
[0009] Preferably, one end of the second spring member is connected to the top of the lower tank body, and the other end of the second spring member is connected to the bottom of the upper tank body.
[0010] Preferably, the first limiting half-ring and the second limiting half-ring are in contact between the upper tank body and the lower tank body.
[0011] Preferably, the first limiting half-ring and the second limiting half-ring are in internal contact with the second spring member, and the first limiting half-ring and the second limiting half-ring are connected by screws.
[0012] Compared with the prior art, the beneficial effects of the present utility model are as follows:
[0013] 1. The reagent bottle for a lysis solution is provided with an upper storage tank component group, a lower storage tank component group and a connection component. The current design of reagent bottles cannot achieve the separate storage of cell lysis solution and PMSF. Operators can only manually add PMSF before use, which increases the operation steps and complexity, and may cause human errors during addition, such as inaccurate dosage. In the corresponding designed upper storage tank component group, lower storage tank component group and connection component, when it is necessary to mix PMSF in the upper tank body and the lysis solution in the lower tank body, by removing the screws connecting between the first limiting half-ring and the second limiting half-ring, separating the first limiting half-ring and the second limiting half-ring and taking them off between the upper tank body and the lower tank body, then pressing the upper tank body, the upper tank body moves downward, the thimble member will contact the sealing ball, the first spring member stores energy and contracts, the sealing ball retracts into the vertical cylinder, the second spring member will store energy and contract. At this time, the PMSF reagent inside the upper tank body will flow through the vertical cylinder, the sealing ball and the thimble member into the lower tank body, so as to be mixed with the lysis solution. At this time, the work of adding PMSF to the lysis solution is completed. And when the pressing on the upper tank body is released, the second spring member releases the stored energy, which will reset the upper tank body and the lower tank body. At this time, the first spring member will also release the stored energy, making the sealing ball block the opening at the bottom of the vertical cylinder again. At this time, the overall device is reset, which is convenient for the next use. The design of the overall device can easily mix PMSF and lysis solution before use, saving the manual addition step;
[0014] 2. The reagent bottle for the lysis solution. The upper storage tank component group is used to store PMSF. During filling, by unscrewing the upper tank lid, the lower tank body is used to fill the lysis solution. Inverting the whole device and unscrewing the lower tank lid, the lysis solution can be filled through the opening on the lower tank body. This design allows the device to repeatedly fill the reagent, improving economic efficiency. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 is the overall structural schematic diagram of the present utility model;
[0016] Figure 2 is the structural schematic diagram of the overall cross-section of the present utility model;
[0017] Figure 3 of the present utility model Figure 2 is the structural schematic diagram at position A;
[0018] Figure 4 of the present utility model Figure 1 is the structural schematic diagram at position B;
[0019] Figure 5 is the structural schematic diagram of the overall explosion of the present utility model.
[0020] In the figure: 01, upper storage tank component group; 11, upper tank body; 12, upper tank lid; 02, lower storage tank component group; 21, lower tank body; 22, lower tank lid; 23, lower tank base; 03, connection component; 31, vertical cylinder; 32, first spring member; 33, sealing ball; 34, thimble member; 35, second spring member; 36, first limiting half-ring; 37, second limiting half-ring. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0021] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.
[0022] Please refer to Figures 1-5, an embodiment provided by the present utility model: a reagent bottle for lysate, comprising: an upper storage tank component group 01 for storing PMSF, the upper storage tank component group 01 includes an upper tank body 11 and an upper tank cover 12. A lower storage tank component group 02 is provided at the bottom of the upper storage tank component group 01 for storing lysate. The lower storage tank component group 02 includes a lower tank body 21, a lower tank cover 22 and a lower tank seat 23. A connecting component 03 is provided between the upper storage tank component group 01 and the lower storage tank component group 02 for connecting the upper storage tank component group 01 and the lower storage tank component group 02. The connecting component 03 includes a vertical cylinder 31. A first spring member 32 is connected inside the vertical cylinder 31. One end of the first spring member 32 is connected to a sealing ball 33. A thimble member 34 is provided at the bottom of the sealing ball 33. A second spring member 35 is provided outside the vertical cylinder 31. A first limiting half-ring 36 and a second limiting half-ring 37 are provided outside the second spring member 35.
[0023] The upper tank cover 12 is connected to the bottle mouth of the upper tank body 11 by threads. The bottle mouth of the lower tank body 21 is at the bottom, and the bottle mouth of the lower tank body 21 is connected to the lower tank cover 22 by threads. The lower tank seat 23 is connected to the bottom of the lower tank body 21.
[0024] The vertical cylinder 31 is fixedly connected to the bottom of the upper tank body 11 through a through hole. The first spring member 32 is connected between the vertical cylinder 31 and the sealing ball 33. An opening is provided at the bottom of the vertical cylinder 31, and the bottom of the sealing ball 33 contacts the opening of the vertical cylinder 31. A groove is provided on the side of the vertical cylinder 31, and the groove on the side of the vertical cylinder 31 is used to communicate the inside of the vertical cylinder 31 with the inside of the upper tank body 11.
[0025] The thimble member 34 is connected to the top of the lower tank body 21 through a bracket. An opening is provided at the position of the lower tank body 21 corresponding to the vertical cylinder 31, and the vertical cylinder 31 contacts the opening of the lower tank body 21.
[0026] One end of the second spring member 35 is connected to the top of the lower tank body 21, and the other end of the second spring member 35 is connected to the bottom of the upper tank body 11.
[0027] The first limiting half-ring 36 and the second limiting half-ring 37 are in contact between the upper tank body 11 and the lower tank body 21.
[0028] The first limiting half-ring 36 and the second limiting half-ring 37 are in internal contact with the second spring member 35. The first limiting half-ring 36 and the second limiting half-ring 37 are connected by screws. The first limiting half-ring 36 and the second limiting half-ring 37 are connected to the outside of the second spring member 35. The first limiting half-ring 36 and the second limiting half-ring 37 are between the upper tank body 11 and the lower tank body 21. Due to the existence of the first limiting half-ring 36 and the second limiting half-ring 37, the upper tank body 11 cannot be pressed down. Therefore, when not mixing, the first limiting half-ring 36 and the second limiting half-ring 37 can prevent accidental contact and possible inversion of the reagent mixture.
[0029] Working principle: The current reagent bottle design cannot achieve the separate storage of cell lysate and PMSF. Operators can only manually add PMSF before use, which increases the operation steps and complexity, and may cause human errors during addition, such as inaccurate dosage. In the upper storage tank component group 01, lower storage tank component group 02 and connection component 03 of the corresponding design, when it is necessary to mix PMSF in the upper tank body 11 and the lysate in the lower tank body 21, by removing the screw connecting between the limit half ring one 36 and the limit half ring two 37, separating the limit half ring one 36 and the limit half ring two 37 and removing them from between the upper tank body 11 and the lower tank body 21. Subsequently, press the upper tank body 11, the upper tank body 11 moves downward, the thimble part 34 will contact the sealing ball 33, the first spring part 32 stores energy and contracts, the sealing ball 33 retracts into the vertical cylinder 31, and the second spring part 35 will store energy and contract. At this time, the PMSF reagent inside the upper tank body 11 will flow through the vertical cylinder 31, pass through the sealing ball 33 and the thimble part 34 and flow into the lower tank body 21, so as to be mixed with the lysate. At this time, the work of adding PMSF to the lysate is completed. And when the pressing on the upper tank body 11 is released, the second spring part 35 releases the stored energy, which will reset the upper tank body 11 and the lower tank body 21. At this time, the first spring part 32 will also release the stored energy, making the sealing ball 33 block the opening position at the bottom of the vertical cylinder 31 again. At this time, the overall device is reset, which is convenient for the next use. The design of the overall device can easily mix PMSF and lysate before use, saving the manual addition step. The upper storage tank component group 01 is used to store PMSF. When filling, by unscrewing the upper tank cover 12, the lower tank body 21 is used to fill the lysate. Invert the overall device and unscrew the lower tank cover 22, and the lysate can be filled through the opening on the lower tank body 21. This design allows the device to repeatedly fill the reagent, improving economic benefits.
[0030] For those skilled in the art, it is obvious that the present utility model is not limited to the details of the above exemplary embodiments, and can be implemented in other specific forms without departing from the spirit or basic characteristics of the present utility model. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present utility model is defined by the appended claims rather than the above description. Therefore, it is intended to embrace all changes falling within the meaning and scope of the equivalent elements of the claims in the present utility model. Any reference signs in the claims should not be regarded as limiting the claimed rights.
Claims
1. A reagent bottle for a lysate, characterized in that: include: An upper storage tank component group (01), the upper storage tank component group (01) is used to store PMSF, the upper storage tank component group (01) comprises an upper tank body (11) and an upper tank cover (12), a lower storage tank component group (02) is arranged at the bottom of the upper storage tank component group (01), the lower storage tank component group (02) is used to store lysate, the lower storage tank component group (02) comprises a lower tank body (21), a lower tank cover (22) and a lower tank seat (23), a connecting assembly (23) is arranged between the upper storage tank component group (01) and the lower storage tank component group (02) 03), the connection assembly (03) is used to connect the upper storage tank component group (01) and the lower storage tank component group (02), the connection assembly (03) comprises a vertical cylinder (31), the interior of the vertical cylinder (31) is connected with a spring component 1 (32), one end of the spring component 1 (32) is connected with a sealing ball (33), the bottom of the sealing ball (33) is provided with a pin component (34), the exterior of the vertical cylinder (31) is provided with a spring component 2 (35), the exterior of the spring component 2 (35) is provided with a limiting semi-ring 1 (36) and a limiting semi-ring 2 (37).
2. A reagent bottle for a lysate according to claim 1, characterized in that: The upper tank cover (12) is connected to the bottle mouth of the upper tank body (11) through a thread, the bottle mouth of the lower tank body (21) is at the bottom, and the bottle mouth of the lower tank body (21) is connected to the lower tank cover (22) through a thread, and the lower tank seat (23) is connected to the bottom of the lower tank body (21).
3. A reagent bottle for a lysate according to claim 1, characterized in that: The vertical cylinder (31) is fixedly connected to the bottom of the upper tank body (11) through a through hole, the spring member 1 (32) is connected between the vertical cylinder (31) and the sealing ball (33), the bottom of the vertical cylinder (31) is provided with an opening, and the bottom of the sealing ball (33) contacts the opening of the vertical cylinder (31), and the side of the vertical cylinder (31) is provided with a groove, and the side groove of the vertical cylinder (31) is used to allow the vertical cylinder (31) and the interior of the upper tank body (11) to communicate.
4. A reagent bottle for a lysate according to claim 1, characterized in that: The ejector pin (34) is connected to the top of the lower tank body (21) via a bracket. The lower tank body (21) is provided with an opening at a position corresponding to the vertical cylinder (31), and the vertical cylinder (31) and the opening of the lower tank body (21) are in contact.
5. The reagent bottle for a lysate according to claim 1, characterized in that: One end of the second spring member (35) is connected to the top of the lower tank body (21), and the other end of the second spring member (35) is connected to the bottom of the upper tank body (11).
6. The reagent bottle for a lysate according to claim 1, characterized in that: The first limiting half ring (36) and the second limiting half ring (37) are in contact between the upper tank body (11) and the lower tank body (21).
7. The reagent bottle for a lysate according to claim 1, characterized in that: The first limiting half ring (36) and the second limiting half ring (37) are in internal contact with the second spring member (35), and the first limiting half ring (36) and the second limiting half ring (37) are connected by screws.