Hanging support for stem cell culture

Abstract

The utility model relates to the field of stem cell culture, and discloses a suspension bracket for stem cell culture, which comprises a shell, the bottom frame is installed in the shell, the bottom frame is used for containing stem cells, and the bottom frame is arranged to be capable of moving in the shell and used for adjusting the position of the bottom frame in the shell; and the top frame is mounted in the shell, and the top frame is arranged above the bottom frame. According to the utility model, by designing the combined structure of the top frame and the bottom frame, the bottom plate of the bottom frame is used for directly bearing cells, and the top plate of the top frame is used for effectively limiting the movement range of the cells, so that the cells are prevented from being rushed open or brought out of the bracket due to hydrodynamic force or buoyancy in the culture process; the stability and safety of cells in the culture process are guaranteed, the relative closed space formed between the top plate and the bottom plate is beneficial to maintaining the constancy of the culture environment, and interference of external factors on cell growth and differentiation is reduced.

Description

Technical Field

[0001] The utility model relates to the field of stem cell culture, in particular to a suspension bracket for stem cell culture. Background Art

[0002] The hanging scaffold used in stem cell culture is an innovative cell culture technology that aims to simulate the three-dimensional growth environment of cells in the body to provide culture conditions that are closer to physiological conditions.

[0003] The publication number CN215480973U disclosed a hanging cell culture rack for hanging in a cell culture dish, comprising a bottom plate and a plurality of hanging ear columns arranged on the upper edge of the bottom plate, wherein the bottom plate is a grid plate so that the culture fluid in the cell culture dish can pass through the bottom plate to contact the cells, and the size of the bottom plate is smaller than the size of the cell culture dish so as to be placed in the cell culture dish, and the hanging ear columns include hanging ear plates extending outward so as to be hung on the side walls of the cell culture dish.

[0004] Existing hanging brackets usually only have a bottom plate for placing cells. When the bracket is hung in a culture dish and the culture fluid contacts the cells through the bottom plate, the cells are easily washed away due to the dynamic effect of the fluid. In some cases, the cells may even be taken out of the bracket due to the buoyancy. This not only affects the fixation effect of the cells, but may also have an adverse effect on the growth and differentiation of the cells. Moreover, when adjusting the position of the bottom plate in the culture dish, only the overall position of the hanging bracket can be adjusted, which is more cumbersome to adjust. Utility Model Content

[0005] In order to solve the above-mentioned problems, the present invention is implemented through the following technical solutions:

[0006] A hanging bracket for stem cell culture comprises: an outer shell; a bottom frame installed in the outer shell, the bottom frame is used to place stem cells, the bottom frame is configured to be movable in the outer shell, and is used to adjust the position of the bottom frame in the outer shell; a top frame installed in the outer shell, the top frame is arranged above the bottom frame, the top frame is used to limit the range of movement of the stem cells and prevent the stem cells from escaping from the outer shell, the top frame is configured to be movable in the outer shell, and is used to adjust the distance between the top frame and the bottom frame.

[0007] The shell comprises: a groove, which is arranged on the inner wall of the shell; and a plurality of limiting grooves, which are arranged on the side wall of the groove and are distributed in a linear array.

[0008] The bottom frame includes: a limit block installed on the bottom frame, the limit block is connected in the limit groove, and when the limit block is in the limit groove, the bottom frame is limited in the shell, and when the limit block slides out of the limit groove and enters the groove, the bottom frame can move in the shell; a bottom plate installed on the bottom frame, and the bottom plate is used to place stem cells.

[0009] The top frame includes: a top plate, which is installed on the top frame and is used to limit the activity range of stem cells.

[0010] It further includes: an adjusting mechanism, which is installed on the outer shell, the bottom frame is connected to the adjusting mechanism, and the adjusting mechanism is used to adjust the position of the bottom frame inside the outer shell; two ear plates, which are installed on the outer shell.

[0011] The adjusting mechanism includes: a fixed rod, which is installed on the outer shell; a moving rod, which is installed on the fixed rod and is set to move on the fixed rod, one end of the moving rod is connected to the top frame; a screw rod, which is installed on the fixed rod, the screw rod is connected to the moving rod, and the screw rod is used to control the movement of the moving rod on the fixed rod when rotating; a knob, which is connected to the top end of the fixed rod, and one end of the screw rod is connected to the knob.

[0012] The ear plate includes: a moving plate, which is installed on the ear plate; a side plate, which is installed on one side of the moving plate; a hanging head, which is installed on the side plate.

[0013] The hanging head includes: a hanging groove, which is opened at the bottom of the hanging head.

[0014] The utility model provides a hanging bracket for stem cell culture. Compared with the prior art, it has the following beneficial effects:

[0015] 1. By designing the combined structure of the top frame and the bottom frame, not only the bottom plate of the bottom frame is directly used to carry cells, but also the activity range of cells is effectively limited by the top plate of the top frame, preventing cells from being washed away or carried out of the bracket due to hydrodynamic or buoyancy effects during the culture process. The dual protection mechanism significantly enhances the fixing effect of cells and ensures the stability and safety of cells during the culture process.

[0016] 2. The relatively enclosed space formed between the top plate and the bottom plate helps to maintain the constancy of the culture environment, reduces the interference of external factors on cell growth and differentiation, and by adjusting the distance between the top frame and the bottom frame, the space size of the culture environment can be flexibly controlled, further optimizing the growth conditions of cells.

[0017] 3. By rotating the bottom frame, the limiting state of the bottom frame inside the outer shell can be controlled, facilitating the direct adjustment of the position of the bottom frame inside the outer shell without moving the entire hanging bracket, improving the accuracy and convenience of adjustment. The design of the ear plate and the hanging head thereon also provides multiple hanging methods for the bracket, enabling it to be conveniently hung on culture dishes or experimental tables of different sizes, enhancing the adaptability and flexibility of the bracket. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a three-dimensional structural schematic diagram proposed by the utility model.

[0019] Figure 2 This is a three-dimensional structure schematic diagram from another perspective proposed by the present utility model.

[0020] Figure 3 This is a cross-sectional structure schematic diagram proposed by the present utility model.

[0021] Figure 4 This is a structure schematic diagram of the bottom frame and the top frame proposed by the present utility model.

[0022] Figure 5 This is a cross-sectional structure schematic diagram of the outer shell proposed by the present utility model.

[0023] Figure 6 This is a structure schematic diagram of the adjusting mechanism proposed by the present utility model.

[0024] The reference numerals in the figure are as follows:

[0025] 1. Outer shell; 101. Groove; 102. Limit groove;

[0026] 2. Bottom frame; 201. Bottom plate; 202. Limit block;

[0027] 3. Top frame; 301. Top plate;

[0028] 4. Adjusting mechanism; 401. Fixed rod; 402. Moving rod; 403. Screw; 404. Knob;

[0029] 5. Ear plate; 501. Moving plate; 502. Side plate; 503. Suspension head; 504. Suspension groove. Detailed implementation manners

[0030] To make the objectives, technical solutions and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments of the present utility model are described clearly and completely. Apparently, the described embodiments are some, but not all, of the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0031] Refer to Figures 1-6, A hanging bracket for stem cell culture, comprising: a housing 1, which serves as the main structure of the entire bracket, providing a necessary protective barrier to prevent interference from the external environment during the stem cell culture process. The internally designed groove 101 and limit groove 102 structures provide precise tracks for the movement and positioning of the bottom frame 2, ensuring the stability of the bracket and the accuracy of adjustment; a bottom frame 2, installed inside the housing 1, used to place stem cells, and the bottom frame 2 is set to be movable inside the housing 1 for adjusting the position of the bottom frame 2 inside the housing 1; a top frame 3, installed inside the housing 1, located above the bottom frame 2, used to limit the activity range of stem cells to prevent stem cells from detaching from the housing 1, and the top frame 3 is set to be movable inside the housing 1 for adjusting the distance between the top frame 3 and the bottom frame 2. The design of the top plate 301 of the top frame 3 effectively limits the activity range of stem cells, preventing stem cells from detaching from the housing 1 during the culture process due to excessive activity, ensuring the continuity and success rate of the culture process. The presence of the top plate 301 also forms a relatively enclosed space, helping to maintain the constancy and sterility of the culture environment.

[0032] Refer to Figure 3 and Figure 5 , The housing 1 includes: a groove 101, opened on the inner wall of the housing 1; a plurality of limit grooves 102, opened on the side wall of the groove 101, distributed in a linear array.

[0033] Refer to Figure 3 and Figure 4 , The bottom frame 2 includes: a limit block 202, installed on the bottom frame 2, and the limit block 202 is connected in the limit groove 102. When the limit block 202 is in the limit groove 102, the bottom frame 2 is limited inside the housing 1. When the limit block 202 slides out of the limit groove 102 and enters the groove 101, the bottom frame 2 can move inside the housing 1; a bottom plate 201, installed on the bottom frame 2, used to place stem cells. The bottom frame 2 cooperates with the limit groove 102 of the housing 1 through the limit block 202 to achieve a flexible switch between fixation and movement of the bottom frame 2. When the limit block 202 is embedded in the limit groove 102, the bottom frame 2 is firmly fixed inside the housing 1 to ensure stability during the stem cell culture process; when the limit block 202 slides out of the limit groove 102 and enters the groove 101, the bottom frame 2 can move freely inside the housing 1, facilitating the adjustment of the position of stem cells according to culture requirements. The design of the bottom plate 201 directly bears the stem cells, providing a safe and stable growth environment for the stem cells.

[0034] Refer to Figure 4 , The top frame 3 includes: a top plate 301, installed on the top frame 3, used to limit the activity range of stem cells.

[0035] Refer to Figure 3 and Figure 6, The adjusting mechanism 4 is installed on the outer shell 1. The bottom frame 2 is connected to the adjusting mechanism 4. The adjusting mechanism 4 is used to adjust the position of the bottom frame 2 within the outer shell 1. The adjusting mechanism 4 includes: a fixed rod 401 installed on the outer shell 1; a moving rod 402 installed on the fixed rod 401 and configured to move on the fixed rod 401. One end of the moving rod 402 is connected to the top frame 3; a screw rod 403 installed on the fixed rod 401. The screw rod 403 is connected to the moving rod 402 and is used to control the movement of the moving rod 402 on the fixed rod 401 when rotating; a knob 404 connected to the top end of the fixed rod 401. One end of the screw rod 403 is connected to the knob 404. Through the precise cooperation of the fixed rod 401, the moving rod 402, the screw rod 403 and the knob 404, the adjusting mechanism 4 realizes the precise adjustment of the position of the bottom frame 2. The user only needs to simply rotate the knob 404, and the transmission of the screw rod 403 can drive the moving rod 402 to move on the fixed rod 401, thereby driving the bottom frame 2 to move up and down within the outer shell 1. It is not only easy to operate but also has high adjustment accuracy, and can meet the different spatial position requirements for different stem cell cultures.

[0036] Refer to Figure 2 and Figure 3 , Two ear plates 5 are installed on the outer shell 1. The ear plate 5 includes: a moving plate 501 installed on the ear plate 5; a side plate 502 installed on one side of the moving plate 501; a hanging head 503 installed on the side plate 502. The hanging head 503 includes: a hanging groove 504 opened at the bottom of the hanging head 503. The design of the ear plate 5 and the moving plate 501, the side plate 502 and the hanging head 503 thereon provides a convenient hanging and fixing method for the entire bracket. The hanging groove 504 at the bottom of the hanging head 503 can easily hang on the culture dish. The moving plate 501 can move flexibly on the ear plate 5, and the bracket can be hung on culture dishes of different sizes, which not only improves the flexibility of the bracket but also facilitates the user's usage requirements in different scenarios. The design of the ear plate 5 also enhances the overall structural strength of the bracket, making it more stable and reliable.

[0037] During use, place the medium required for stem cell culture on the bottom plate 201 of the bottom frame 2 to ensure the safety and stability of the stem cells. Insert the bottom frame 2 from the bottom of the outer shell 1. The limit block 202 moves within the groove 101. After adjusting the bottom frame 2 to the appropriate position, rotate the bottom frame 2 to drive the limit block 202 into the corresponding limit groove 102. At this time, the bottom frame 2 is firmly fixed within the outer shell 1, providing a stable initial environment for stem cell culture. The top frame 3 is installed within the outer shell 1, ensuring that the top plate 301 of the top frame 3 is located above the bottom frame 2 but maintaining a certain initial distance to limit the activity range of the stem cells. Hang the hanging bracket on the culture dish or experimental table through the hanging head 503 on the ear plate 5, and adjust the position of the moving plate 501 to adapt to containers or experimental tables of different sizes. If it is necessary to adjust the position of the stem cells during culture, rotate the bottom frame 2. The bottom frame 2 drives the limit block 202 to slide out of the limit groove 102 and into the groove 101. At this time, the bottom frame 2 is unlocked and can move freely within the outer shell 1, and the position of the bottom frame 2 can be adjusted. Rotate the knob 404 on the adjustment mechanism 4. The knob 404 drives the screw rod 403 to rotate. The rotation of the screw rod 403 will be converted into the linear movement of the moving rod 402 on the fixed rod 401. Since one end of the moving rod 402 is connected to the top frame 3, the movement of the moving rod 402 will drive the top frame 3 to move up and down, indirectly adjusting the position of the top frame 3 within the outer shell 1, and the distance between the top plate 301 and the bottom plate 201 can be adjusted. The top plate 301 of the top frame 3 effectively limits the activity range of the stem cells, preventing them from detaching from the outer shell 1, and at the same time forms a relatively enclosed space, which helps to maintain the constancy of the culture environment.

[0038] In summary, compared with the prior art, the following beneficial effects are achieved:

[0039] By designing the combined structure of the top frame 3 and the bottom frame 2, not only does the bottom plate 201 of the bottom frame 2 directly carry the cells, but also the top plate 301 of the top frame 3 effectively limits the activity range of the cells, preventing the cells from being washed away or carried out of the bracket due to hydrodynamic or buoyant forces during culture. The dual protection mechanism significantly enhances the fixing effect of the cells and ensures the stability and safety of the cells during culture.

[0040] The relatively enclosed space formed between the top plate 301 and the bottom plate 201 helps to maintain the constancy of the culture environment, reducing the interference of external factors on cell growth and differentiation. By adjusting the distance between the top frame 3 and the bottom frame 2, the spatial size of the culture environment can be flexibly controlled, further optimizing the growth conditions of the cells.

[0041] By rotating the bottom frame 2, the limited state of the bottom frame 2 within the outer shell 1 can be controlled, facilitating the direct adjustment of the position of the bottom frame 2 within the outer shell 1 without moving the entire suspension bracket, improving the accuracy and convenience of adjustment. The design of the ear plate 5 and the suspension head 503 thereon also provides multiple suspension methods for the bracket, enabling it to be conveniently suspended on culture dishes or experimental benches of different sizes, enhancing the adaptability and flexibility of the bracket.

[0042] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "comprising an..." does not exclude the existence of additional identical elements in the process, method, article or device comprising the said element.

[0043] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A hanging support for stem cell culture, characterized in that: include: Housing (1); A bottom frame (2) is installed in the outer shell (1), the bottom frame (2) is used to place stem cells, and the bottom frame (2) is configured to be movable in the outer shell (1) to adjust the position of the bottom frame (2) in the outer shell (1); A top frame (3) is installed in the outer shell (1). The top frame (3) is arranged above the bottom frame (2). The top frame (3) is used to limit the range of movement of the stem cells and prevent the stem cells from escaping from the outer shell (1). The top frame (3) is arranged to be movable in the outer shell (1) and is used to adjust the distance between the top frame (3) and the bottom frame (2).

2. A stem cell culture suspension support according to claim 1, characterized in that: The housing (1) comprises: A groove (101) is formed on the inner wall of the housing (1); A plurality of limiting grooves (102) are provided on the side wall of the groove (101) and are distributed in a linear array.

3. A stem cell culture suspension support according to claim 2, characterized in that: The bottom frame (2) comprises: a limit block (202) mounted on the bottom frame (2); the limit block (202) is connected to the limit slot (102); the limit block (202) is configured to limit the bottom frame (2) in the housing (1) when in the limit slot (102); and when the limit block (202) slides out of the limit slot (102) and enters the groove (101), the bottom frame (2) can move in the housing (1); A bottom plate (201) is mounted on the bottom frame (2), and the bottom plate (201) is used to place stem cells.

4. A stem cell culture suspension support according to claim 1, characterized in that: The top frame (3) comprises: A top plate (301) is mounted on the top frame (3), and the top plate (301) is used to limit the range of activity of stem cells.

5. A stem cell culture suspension support according to claim 1, characterized in that: Also includes: An adjustment mechanism (4) is mounted on the outer shell (1), the bottom frame (2) is connected to the adjustment mechanism (4), and the adjustment mechanism (4) is used to adjust the position of the bottom frame (2) in the outer shell (1); Two ear plates (5) are mounted on the housing (1).

6. A stem cell culture suspension support according to claim 5, characterized in that: The regulating mechanism (4) comprises: A fixing rod (401) mounted on the housing (1); A movable rod (402) is mounted on the fixed rod (401) and is configured to move on the fixed rod (401); one end of the movable rod (402) is connected to the top frame (3); A screw rod (403) is mounted on the fixed rod (401), the screw rod (403) is connected to the moving rod (402), and the screw rod (403) is used to control the moving rod (402) to move on the fixed rod (401) when rotating; The knob (404) is connected to the top end of the fixing rod (401), and one end of the screw rod (403) is connected to the knob (404).

7. A stem cell culture suspension support according to claim 5, characterized in that: The ear plate (5) comprises: A movable plate (501) mounted on the ear plate (5); A side plate (502) mounted on one side of the movable plate (501); The suspension head (503) is mounted on the side plate (502).

8. A stem cell culture suspension support according to claim 7, characterized in that: The suspension head (503) comprises: The hanging groove (504) is formed at the bottom of the hanging head (503).

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