Pretreatment system of multi-mode flow splitting elution instrument

Abstract

The utility model provides a pretreatment system of a multi-mode flow type cracking elution instrument, which belongs to the technical field of cell experiment equipment and comprises a central control system, an operation table, a first mechanical arm module, an uncovering auxiliary module, a suction head storage device, a test tube storage device, a second mechanical arm module, an experiment incubation device and a reagent storage device, the experiment incubation device comprises a magnetic adsorption unit, a constant-temperature incubation unit and a cleaning head module, the magnetic adsorption unit is arranged below the constant-temperature incubation unit, an incubation cabin for accommodating a flow type tube is arranged in the constant-temperature incubation unit, and the cleaning head module is arranged above the incubation cabin; according to the pretreatment system of the multi-mode flow type cracking elution instrument, not only can the flow type pretreatment operation of cells be realized, but also the flow type pretreatment operation of microspheres can be carried out; the overall structure is compact and the size is small.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell experiment equipment, and particularly relates to a pretreatment system for a multi-mode flow cytometer lysis and elution instrument. Background Technique

[0002] Flow cytometry is a technique for cell analysis and counting. By introducing a cell suspension into a flow cytometer, the physical and chemical properties of cells are used for identification, classification, and counting. It is based on injecting a cell suspension into a flow cytometer, allowing cells to pass through a laser beam scanning area one by one, and obtaining multi-parameter information of cells through the scattering, fluorescence, and absorption characteristics of the laser, including cell size, morphology, color, and the expression level of surface markers, etc. Flow cytometry has the characteristics of high throughput, high sensitivity, high precision, and high resolution, and can quickly obtain a large amount of cell data. In the field of life science research, it is widely used in immunology, cell biology, oncology, etc. to study cell functions, phenotypes, and states, analyze processes such as cell proliferation, apoptosis, and differentiation, and study mechanisms such as cell immune responses and signal transduction. In addition, flow cytometry can be combined with other technologies to further expand its application scope. In clinical diagnosis, flow cytometry is of great significance, such as immunophenotyping analysis, leukemia diagnosis, immunotherapy monitoring, etc.

[0003] In short, flow cytometry is a powerful cell analysis technique that can provide rich cell information and is widely used in the fields of life science research and clinical diagnosis.

[0004] The Luminex platform is a multi-parameter flow cytometry analysis technique that can detect multiple molecular markers simultaneously. It combines flow cytometry and fluorescence microsphere technology and can analyze multiple biomarkers in one experiment, thus providing more comprehensive information.

[0005] The working principle of the Luminex platform is to bind microspheres with different fluorescent labels to specific antibodies or nucleic acid probes to form fluorescently labeled microsphere probes. These microsphere probes are specific and can bind to the molecular markers in the sample to be tested. After the molecular markers in the sample to be tested bind to the fluorescently labeled microsphere probes, they can be detected and analyzed by a flow cytometer.

[0006] The Luminex platform features high throughput, high sensitivity, and high specificity. It can simultaneously detect dozens or hundreds of molecular markers and obtain a large amount of data in a relatively short period. The Luminex platform is widely used in biomedical research and clinical diagnosis. In biomedical research, the Luminex platform can be used to study disease mechanisms, evaluate the efficacy of drug treatment, and study protein interactions. In clinical diagnosis, the Luminex platform can be used to detect biomarkers in serum to assist in the early diagnosis of diseases, monitor disease progression, and evaluate treatment efficacy.

[0007] Currently, the development of flow cytometry enables cell analysis and detection, as well as high-throughput multi-parameter microsphere flow cytometry detection. Although there are many diversities in flow cytometry detection, the clinical-related flow cytometry sample pretreatment system is still in a relatively single state. It can only handle the wash-free related items in cell analysis detection pretreatment, such as the sample pretreatment for lymphocyte subset detection.

[0008] The entire flow cytometry sample pretreatment method can be divided into two types, one is cell flow cytometry pretreatment, and the other is microsphere flow cytometry pretreatment.

[0009] The main operation steps of cell flow cytometry pretreatment are as follows: 1) Add antibody detection reagent; 2) Add the mixed whole blood sample to be tested; 3) Incubate in the dark for 10 - 15 minutes; 4) Add hemolysin to lyse red blood cells; 5) Oscillate and mix well, and incubate in the dark for 10 - 15 minutes to complete the experiment.

[0010] The main difference between microsphere flow cytometry pretreatment and cell pretreatment lies in the detection reagent. Microsphere flow cytometry pretreatment uses magnetic-coded microspheres with antibodies for detection. Therefore, during the entire experiment, magnetic adsorption operations need to be added, and the waste liquid generated during the experiment needs to be washed away.

[0011] Current experimental equipment cannot be compatible with both cell flow cytometry (wash-free related items) and microsphere flow cytometry pretreatment operations. Summary of the Utility Model

[0012] In view of the problems existing in the above-mentioned prior art, the present utility model provides a pretreatment system for a multi-mode flow cytometry lysis and elution instrument. The technical problem to be solved by the present utility model is to provide a system that can perform both cell flow cytometry pretreatment operations and microsphere flow cytometry pretreatment operations.

[0013] To solve the above technical problems, the present utility model provides a pretreatment system for a multi-mode flow cytometry lysis and elution instrument, including a central control system, an operation console, and a first robotic arm module, an open lid assistance module, a pipette tip storage device, a test tube storage device, a second robotic arm module, an experimental incubation device, and a reagent storage device arranged above the operation console;

[0014] Both the first robotic arm module and the second robotic arm module are arranged above the lid opening assistance module, the pipette tip storage device, the test tube storage device, the experimental incubation device, and the reagent storage device; the first robotic arm module, the lid opening assistance module, the second robotic arm module, and the experimental incubation device are all electrically connected to the central control system;

[0015] Both the first robotic arm module and the second robotic arm module can move along the X-axis, Y-axis, and Z-axis;

[0016] The experimental incubation device includes a magnetic adsorption unit, a constant temperature incubation unit, and a cleaning head module. The magnetic adsorption unit is arranged below the constant temperature incubation unit. An incubation chamber for accommodating flow cytometry tubes is arranged inside the constant temperature incubation unit. The cleaning head module is arranged above the incubation chamber;

[0017] The first robotic arm module grabs a test tube on the test tube storage device and places it inside the lid opening assistance module. The first robotic arm module opens the lid of the test tube. The second robotic arm module transfers the sample solution in the test tube into the flow cytometry tube in the incubation chamber for oscillating incubation. If a microsphere flow cytometry pretreatment operation is performed, after the oscillating incubation, the central control system controls the magnetic adsorption unit to adsorb the magnetic microspheres in the flow cytometry tube to the tube wall, and the central control system controls the cleaning head module to aspirate the waste liquid in the flow cytometry tube to achieve the cleaning of the flow cytometry tube.

[0018] Further, the magnetic adsorption unit includes a magnetic component, a magnetic component slide rail, and a magnetic component driving device. The magnetic component driving device is connected to one side of the magnetic component; the magnetic component is slidably connected to the magnetic component slide rail.

[0019] Further, the magnetic component includes a plate body and magnetic plates. A plurality of magnetic plates are arranged in parallel above the plate body, and through holes are arranged between the magnetic plates.

[0020] Further, the constant temperature incubation unit includes heating components arranged opposite to each other. An incubation chamber is formed between the heating components, and a flow cytometry tube rack is arranged inside the incubation chamber.

[0021] Further, the cleaning head module includes a moving component and a steel needle component. The steel needle component is arranged on one side of the moving component. A slide rail is arranged on the experimental incubation device, and the slide rail is slidably connected to the moving component.

[0022] Further, a washing device is arranged on one side of the constant temperature incubation unit, and a washing chamber is arranged inside the washing device.

[0023] Further, the experimental incubation device includes a sample addition window.

[0024] Further, the sample addition window is arranged above the incubation chamber, and a cover plate is arranged at the sample addition window.

[0025] Further, the experimental incubation device includes an eccentric oscillation module.

[0026] Further, the test tube storage device includes a tube rack, a placement table, and an oscillation mechanism. A plurality of test tubes loaded with samples to be tested are arranged inside the tube rack. The tube rack is arranged above the placement table, and the oscillation mechanism is connected below the placement table.

[0027] In the current market, most products of the same type use an automated lysis instrument as the core and are mainly dedicated to realizing the detection of lymphocyte subset items. Most of these devices perform automated processing for projects that do not require washing; the multi-mode flow sample pretreatment system of the present utility model can realize the constant temperature and light-shielding incubation and cleaning of flow tubes; by setting up an experimental incubation device, the experimental incubation device includes an eccentric oscillation module, a magnetic adsorption unit, a constant temperature incubation unit, and a cleaning head module. The eccentric oscillation module can drive the flow tube to perform an oscillation experiment; the magnetic adsorption unit can adsorb the magnetic microspheres in the flow tube to the tube wall, and the steel needle in the cleaning head module can suck and discharge the waste liquid in the flow tube to achieve washing; a heating component is arranged in the constant temperature incubation unit, which can keep the incubation chamber at a constant incubation temperature; thus, the pretreatment system of the multi-mode flow lysis elution instrument of the present utility model can not only realize the cell flow pretreatment operation, but also perform the microsphere flow pretreatment operation; the overall structure is compact and the volume is small. Description of the Drawings

[0028] Figure 1 is a schematic structural diagram of a multi-mode flow lysis elution instrument pretreatment system of the present utility model Figure 1 。

[0029] Figure 2 is a schematic structural diagram of a multi-mode flow lysis elution instrument pretreatment system of the present utility model Figure 2 。

[0030] Figure 3 is a schematic diagram of the first robotic arm module of a multi-mode flow lysis elution instrument pretreatment system of the present utility model.

[0031] Figure 4 is a schematic diagram of the open lid assist module of a multi-mode flow lysis elution instrument pretreatment system of the present utility model.

[0032] Figure 5 is a schematic diagram of the pipette tip storage device of a multi-mode flow lysis elution instrument pretreatment system of the present utility model.

[0033] Figure 6 is a schematic diagram of the tube rack of a multi-mode flow lysis elution instrument pretreatment system of the present utility model.

[0034] Figure 7 is a schematic diagram of the placement table and the oscillation mechanism of a multi-mode flow lysis elution instrument pretreatment system of the present utility model Figure 1 。

[0035] Figure 8 It is a schematic diagram of the placement table and oscillation mechanism of the pretreatment system of a multi-mode flow cytometer lysis and elution instrument of the present utility model. Figure 2 。

[0036] Figure 9 It is a schematic diagram of the second robotic arm module of the pretreatment system of a multi-mode flow cytometer lysis and elution instrument of the present utility model.

[0037] Figure 10 It is a schematic diagram of the experimental incubation device of the pretreatment system of a multi-mode flow cytometer lysis and elution instrument of the present utility model. Figure 1 。

[0038] Figure 11 It is a schematic diagram of the experimental incubation device of the pretreatment system of a multi-mode flow cytometer lysis and elution instrument of the present utility model. Figure 2 。

[0039] Figure 12 It is a schematic diagram of the experimental incubation device of the pretreatment system of a multi-mode flow cytometer lysis and elution instrument of the present utility model. Figure 3 。

[0040] Figure 13 It is a schematic diagram of the experimental incubation device of the pretreatment system of a multi-mode flow cytometer lysis and elution instrument of the present utility model. Figure 4 。

[0041] Figure 14 It is a schematic diagram of the experimental incubation device of the pretreatment system of a multi-mode flow cytometer lysis and elution instrument of the present utility model. Figure 5 。

[0042] Figure 15 It is a schematic diagram of the magnetic adsorption unit of the pretreatment system of a multi-mode flow cytometer lysis and elution instrument of the present utility model.

[0043] Figure 16 It is a schematic diagram of the heating component of the pretreatment system of a multi-mode flow cytometer lysis and elution instrument of the present utility model.

[0044] Figure 17 It is a schematic diagram of the eccentric oscillation module of the pretreatment system of a multi-mode flow cytometer lysis and elution instrument of the present utility model.

[0045] Among them, 1. First robotic arm module; 2. Lid-opening assistance module; 3. Tip storage device; 4. Test tube storage device; 5. Second robotic arm module; 6. Experimental incubation device; 7. Reagent storage device; 8. Operating table; 9. X-axis module; 10. Cleaning head module; 11. Y-axis module of the first robotic arm module; 12. First Z-axis module; 13. Gripping assembly; 131. Gripping assembly driving device; 132. Gripper; 21. Clamping part; 31. Swing frame; 41. Tube rack; 42. Placement table; 43. Oscillation mechanism; 51. Y-axis module of the second robotic arm module; 52. Second Z-axis module; 53. Pipetting assembly; 531. Second robotic arm module driving device; 532. Pipetting tip; 60. Cover plate; 61. Magnetic component; 611. Plate body; 612. Magnetic plate; 613. Through hole; 62. Magnetic component slide rail; 63. Magnetic component driving device; 64. Heating component; 65. Flow cytometry tube rack; 66. Moving assembly; 67. Steel needle assembly; 68. Slide rail; 69. Washing device; 70. Oscillation seat; 71. Vibration motor; 72. First synchronous pulley; 73. Second synchronous pulley; 661. Moving Z-axis module; 691. Washing cavity. Detailed implementation manners

[0046] To make the objectives, technical solutions and advantages of the embodiments of the present utility model clearer, the technical solutions in the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are some but not all of the embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0047] Therefore, the detailed description of the embodiments of the present utility model provided in the accompanying drawings is not intended to limit the scope of the claimed present utility model, but merely represents selected embodiments of the present utility model. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0048] It should be noted that like reference numerals and letters denote similar items in the following drawings. Therefore, once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0049] In the present utility model, unless otherwise clearly specified and defined, terms such as "installation", "connection", "linkage", "fixation", etc. shall be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or an integral one; it may be a direct connection or an indirect connection through an intermediate medium, and it may be the internal communication of two components or the interaction relationship between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0050] To better understand the purpose, structure and function of the present utility model, the following further describes in detail a pretreatment system for a multi-mode flow cytometer lysis elution instrument in conjunction with the attached Figures 1 - 15 , a pretreatment system for a multi-mode flow cytometer lysis elution instrument of the present utility model.

[0051] Example 1:

[0052] As Figure 1 and Figure 2 , this embodiment provides a pretreatment system for a multi-mode flow cytometer lysis elution instrument, including a central control system, an operation console 8, and a first robotic arm module 1, an open lid assistance module 2, a pipette tip storage device 3, a test tube storage device 4, a second robotic arm module 5, an experimental incubation device 6, and a reagent storage device 7 arranged above the operation console 8;

[0053] Both the first robotic arm module 1 and the second robotic arm module 5 are arranged above the open lid assistance module 2, the pipette tip storage device 3, the test tube storage device 4, the experimental incubation device 6, and the reagent storage device 7; the first robotic arm module 1, the open lid assistance module 2, the second robotic arm module 5, and the experimental incubation device 6 are all electrically connected to the central control system;

[0054] The first robotic arm module 1 and the second robotic arm module 5 move along the X, Y, and Z directions;

[0055] The experimental incubation device 6 includes a magnetic adsorption unit, a constant temperature incubation unit, and a cleaning head module 10. The magnetic adsorption unit is arranged below the constant temperature incubation unit. An incubation chamber for accommodating flow cytometry tubes is arranged inside the constant temperature incubation unit, and the cleaning head module 10 is arranged above the incubation chamber.

[0056] The first robotic arm module 1 grabs a test tube on the test tube storage device 4 and places it inside the open lid assistance module 2. The first robotic arm module 1 opens the lid of the test tube. The second robotic arm module 5 transfers the sample solution in the test tube into the flow cytometry tube in the incubation chamber for oscillating incubation. If a microsphere flow cytometry pretreatment operation is performed, after the oscillating incubation, the central control system controls the magnetic adsorption unit to adsorb the magnetic microspheres in the flow cytometry tube to the tube wall, and the central control system controls the cleaning head module 10 to aspirate the waste liquid in the flow cytometry tube to achieve the cleaning of the flow cytometry tube.

[0057] Example 2:

[0058] As Figure 1 and Figure 2 , this embodiment provides a pretreatment system for a multi-mode flow lysis elution instrument, including a central control system, an operation console 8, and a first robotic arm module 1, an open lid assist module 2, a pipette tip storage device 3, a test tube storage device 4, a second robotic arm module 5, an experimental incubation device 6, and a reagent storage device 7 arranged above the operation console 8;

[0059] Both the first robotic arm module 1 and the second robotic arm module 5 are arranged above the open lid assist module 2, the pipette tip storage device 3, the test tube storage device 4, the experimental incubation device 6, and the reagent storage device 7; the first robotic arm module 1, the open lid assist module 2, the second robotic arm module 5, and the experimental incubation device 6 are all electrically connected to the central control system;

[0060] The movement of the first robotic arm module 1 and the second robotic arm module 5 in the X, Y, and Z directions;

[0061] The experimental incubation device 6 includes a magnetic adsorption unit, a constant temperature incubation unit, and a cleaning head module 10. The magnetic adsorption unit is arranged below the constant temperature incubation unit. An incubation chamber for accommodating a flow tube is arranged inside the constant temperature incubation unit, and the cleaning head module 10 is arranged above the incubation chamber.

[0062] The first robotic arm module 1 grabs a test tube on the test tube storage device 4 and places it inside the open lid assist module 2. The first robotic arm module 1 opens the lid of the test tube. The second robotic arm module 5 transfers the sample solution in the test tube to the flow tube in the incubation chamber for oscillating incubation. If a microsphere flow pretreatment operation is performed, after the oscillating incubation, the central control system controls the magnetic adsorption unit to adsorb the magnetic microspheres in the flow tube to the tube wall, and the central control system controls the cleaning head module 10 to aspirate the waste liquid in the flow tube to achieve the cleaning of the flow tube.

[0063] The difference between this embodiment and the first embodiment lies in:

[0064] As Figures 10 - 15 , the magnetic adsorption unit includes a magnetic component 61, a magnetic component slide rail 62, a magnetic component driving device 63, and a transmission device. After the oscillating incubation is completed, the central control system controls the magnetic component driving device 63. The magnetic component driving device 63 drives the transmission device, and the driving transmission device drives the magnetic component 61 to rise along the magnetic component slide rail 62 to the bottom of the flow tube and maintain a static state for 1 - 2 minutes to ensure that all magnetic microspheres are completely adsorbed on the side wall of the flow tube; the driving transmission device includes a gear and a rack, and the two are meshed and connected.

[0065] The magnetic component 61 includes a plate body 611 and magnetic plates 612. A plurality of magnetic plates 612 are arranged in parallel above the plate body 611. Through holes 613 are provided between the magnetic plates 612. When the magnetic component rises, the bottom of the flow tube is placed in the through holes.

[0066] As Figure 16 , the constant temperature incubation unit includes heating components 64 arranged opposite to each other. An incubation chamber is formed between the heating components 64.

[0067] The heating component 64 is a PI heating film attached to the side wall of the incubation chamber. A flow tube rack 65 is arranged inside the incubation chamber. The central control system controls the heating component 64 to perform a heating operation. When the temperature reaches 37°C ± 1°C, the temperature in the incubation chamber is kept constant, facilitating the microsphere flow cytometry pretreatment operation.

[0068] The cleaning head module 10 includes a moving component 66 and a steel needle component 67. The steel needle component 67 is arranged on one side of the moving component 66. A slide rail 68 is arranged on the experimental incubation device 6, which is slidably connected to the moving component 66;

[0069] A washing device 69 is arranged on one side of the constant temperature incubation unit. The moving component 66 can drive the steel needle component 67 to move along the slide rail 68 on the experimental incubation device 6; the moving component 66 includes a moving Z-axis module 661. The steel needle component 67 can descend along the moving Z-axis module 661 and insert into the washing chamber 691 inside the washing device 69 to clean the steel needle; or insert into the incubation chamber to aspirate the waste liquid in the flow tube.

[0070] A plurality of steel needles are arranged on the steel needle component 67. Preferably, the number of steel needles is 40.

[0071] As Figure 17 , the experimental incubation device 6 includes an eccentric oscillation module, and the eccentric oscillation module can drive the flow tube to oscillate; the eccentric oscillation module includes a vibration motor 71, a first synchronous pulley 72 and a second synchronous pulley 73. The vibration motor 71 is connected to the first synchronous pulley 72. The first synchronous pulley 72 is connected to the second synchronous pulley 73 through a belt. The center of the second synchronous pulley 73 is an eccentric shaft, and the eccentric shaft is connected to an oscillation seat 70. The flow tube rack 65 is placed on the oscillation seat 70. When the vibration motor rotates, it drives the second synchronous pulley 73 to rotate through the belt, causing the eccentric shaft to rotate synchronously, so that the oscillation seat 70 forms an oscillation effect.

[0072] Holes through which the magnetic plates 612 can pass are provided on the oscillation seat 70.

[0073] The experimental incubation device 6 includes a sample addition window, and the sample addition window is arranged above the incubation chamber. A cover plate 60 is arranged at the sample addition window. When adding samples, the cover plate 60 is opened, and when incubating, the cover plate 60 is closed.

[0074] Example 3:

[0075] As Figure 1 and Figure 2 , this embodiment provides a pretreatment system for a multi-mode flow lysis and elution instrument, including a central control system, an operation table 8, and a first robotic arm module 1, an open lid assisting module 2, a pipette tip storage device 3, a test tube storage device 4, a second robotic arm module 5, an experimental incubation device 6, and a reagent storage device 7 arranged above the operation table 8;

[0076] Both the first robotic arm module 1 and the second robotic arm module 5 are arranged above the open lid assisting module 2, the pipette tip storage device 3, the test tube storage device 4, the experimental incubation device 6, and the reagent storage device 7; the first robotic arm module 1, the open lid assisting module 2, the second robotic arm module 5, and the experimental incubation device 6 are all electrically connected to the central control system;

[0077] The first robotic arm module 1 and the second robotic arm module 5 can move along the X direction, Y direction, and Z direction;

[0078] The experimental incubation device 6 includes a magnetic adsorption unit, a constant temperature incubation unit, and a cleaning head module 10. The magnetic adsorption unit is arranged below the constant temperature incubation unit. An incubation chamber for accommodating flow tubes is arranged inside the constant temperature incubation unit, and the cleaning head module 10 is arranged above the incubation chamber.

[0079] The first robotic arm module 1 grabs a test tube on the test tube storage device 4 and places it inside the open lid assisting module 2. The first robotic arm module 1 opens the lid of the test tube. The second robotic arm module 5 transfers the sample solution in the test tube to the flow tube in the incubation chamber for oscillating incubation. If a microsphere flow pretreatment operation is performed, after the oscillating incubation, the central control system controls the magnetic adsorption unit to adsorb the magnetic microspheres in the flow tube to the tube wall, and the central control system controls the cleaning head module 10 to aspirate the waste liquid in the flow tube to achieve the cleaning of the flow tube.

[0080] The difference between this embodiment and the first embodiment is that:

[0081] The first robotic arm module 1 and the second robotic arm module 5 share a set of X-axis modules 9.

[0082] As Figure 3 , the first robotic arm module 1 includes a first robotic arm module Y-axis module 11, a first Z-axis module 12, and a grasping component 13. The grasping component 13 is connected to one side of the first Z-axis module 12. The first Z-axis module 12 is connected to the first robotic arm module Y-axis module 11. The first robotic arm module Y-axis module 11 is connected to the X-axis module 9. The grasping component 13 includes a grasping component driving device 131 and a gripper 132. The central control system controls the grasping component driving device 131 to control the gripper 132 to clamp and release the test tube. The number of grippers 132 is two groups, and two test tubes can be clamped at a time.

[0083] As Figure 9 shown in Figure 9 , the second robotic arm module 5 includes a second robotic arm module Y-axis module 51, a second Z-axis module 52, and a pipetting assembly 53. The pipetting assembly 53 is connected to one side of the second Z-axis module 52, the second Z-axis module 52 is connected to the second robotic arm module Y-axis module 51, the second robotic arm module Y-axis module 51 is connected to the X-axis module 9, the pipetting assembly 53 includes a second robotic arm module driving device 531 and a pipetting head 532, and the central control system controls the second robotic arm module driving device 531 to control the pipetting head 532 to install and remove the pipette tips.

[0084] As Figure 4 shown in Figure 4 , the tube opening assistance module 2 includes a clamping portion 21 in which the test tube is placed for fixed clamping; a camera is provided on the tube opening assistance module 2 to monitor the tube opening condition of the test tube.

[0085] As Figure 5 shown in Figure 5 , the pipette tip storage device 3 includes a rack 31 on which a plurality of pipette tips are placed.

[0086] As Figures 6 - 8 shown in Figures 6 - 8 , the test tube storage device 4 includes a tube rack 41, a placement table 42, and an oscillation mechanism 43. A plurality of test tubes loaded with samples to be tested are arranged inside the tube rack 41. The tube rack 41 is arranged above the placement table 42, and the oscillation mechanism 43 is connected below the placement table 42. Driven by the motor of the oscillation mechanism 43, the sample solution above the placement table 42 can be oscillated and mixed. Preferably, in a single experiment, the maximum throughput of the tube rack 41 is 40 sample collection tubes, which is compatible with different types of collection tubes and 1.5 mL centrifuge tubes or 2.0 mL centrifuge tubes to meet different experimental requirements.

[0087] The main function of the reagent storage device 7 is to ensure the proper storage of reagents and consumables during the project experiment. The currently stored reagents include: antibody reagents, microsphere reagents, quality control blood, hemolysin reagents, and PBS reagents.

[0088] The process of the pre-treatment system of a multi-mode flow cytometry lysis and elution instrument for performing cell flow cytometry pre-treatment operations is as follows:

[0089] After the project program is started, the system software will prompt the staff to place consumables such as pipette tips and reagents at the designated positions;

[0090] After the pre-experiment preparation work is completed, due to project requirements, it is necessary to perform a sample mixing operation on the samples. At this time, the test tube storage device 4 will be started to perform the mixing operation of the samples in the tube rack 41;

[0091] After the sample mixing is completed, the first robotic arm module 1 transfers the sample to the lid-opening assistance module 2. At this time, the camera in the lid-opening assistance module 2 will be turned on to monitor whether there is any abnormality during the entire operation process of the sample from lid-opening to lid-closing. If there is any abnormal behavior (such as failed lid-opening or failed tube lid-closing), it will trigger the central control system to report an error.

[0092] After the lid of the sample to be tested is opened, the second robotic arm module 5 inserts a pipette tip from the pipette tip storage device 3 and then performs a sample pipetting operation, pipetting the sample into the flow tube of the experimental incubation device 6. The second robotic arm module 5 changes the pipette tip every time it performs a pipetting operation.

[0093] After the pipetting is completed, the first robotic arm module 1 executes the lid-closing program and transfers the tube that has completed pipetting to the original position of the tube storage device 4.

[0094] When all the samples in the tube storage device 4 have been added, the second robotic arm module 5 inserts a new pipette tip from the pipette tip storage device 3 again and performs an antibody reagent dispensing operation from the reagent storage device 7.

[0095] When the reagent addition is completed, the experimental incubation device 6 starts to oscillate and mix the sample solution and the reagent in the flow tube, closes the upper cover plate 60, so that the incubation chamber in the entire experimental incubation device 6 is in a light-shielded mode for about 15 minutes.

[0096] When the incubation of the sample and the detection antibody reagent is completed, the second robotic arm module 5 inserts a new pipette tip from the pipette tip storage device 3 again and performs a reagent dispensing of hemolysin from the reagent storage device 7.

[0097] When the reagent addition to the flow tube is completed, the experimental incubation device starts to oscillate and mix the sample solution and the reagent in the flow tube, closes the upper cover plate 60, so that the incubation chamber in the entire experimental incubation device 6 is in a light-shielded mode for about 15 minutes.

[0098] When the experiment stops, take out the flow tube rack 65 from the sampling window at the front end of the experimental incubation device 6 for subsequent flow cytometry detection operations.

[0099] The process of the pretreatment system of a multi-mode flow cytometry lysis and elution instrument of the present utility model during the microsphere flow cytometry pretreatment operation is as follows:

[0100] Compared with the pretreatment operation of cell flow cytometry, the main steps of the microsphere flow cytometry pretreatment are that the sample incubation, incubation chamber heating, magnetic microsphere adsorption, and sample washing operations can be performed in the experimental incubation device 6. The specific related operation process:

[0101] Following the specified operation process, the initial sample addition step is performed by the first robotic arm module 1 and the second robotic arm module 5, which is the same as the sample addition step in the pre-processing operation of flow cytometry.

[0102] After the reagent addition is completed, close the cover plate 60 above the experimental incubation device 6 to make the incubation chamber in a light-shielded environment. Start the eccentric oscillation module of the experimental incubation device 6 to oscillate and mix the samples and reagents in the flow tube. The central control system will control the heating component 64 to perform a heating operation to make the incubation chamber in a constant temperature environment at a temperature of 37°C ± 1°C;

[0103] After the incubation step is completed, the central control system will control the magnetic component 61 to rise from the initial position to the bottom of the flow tube and maintain a static state for 1-2 minutes to ensure that all magnetic coded microspheres are completely adsorbed on the side wall of the flow tube;

[0104] In the experimental cleaning stage, open the cover plate 60, and the central control system controls the cleaning head module 10 to move to the position of the flow tube rack 65. At the same time, the central control system controls the moving component to drive the steel needle component 67 to descend, and multiple steel needles enter the inside of the flow tube. At this time, the central control system starts the negative pressure pump in the cleaning head module 10 to suck the residual waste liquid inside the flow tube and then discharge it through the pipeline inside the cleaning head module 10. During this process, the magnetic component 61 remains stationary and does not perform any movement;

[0105] After all the waste liquid is sucked and discharged, the central control system will control the moving component 66 to drive the steel needle component 67 to withdraw from the inside of the flow tube, and at the same time control the moving component 66 to drive the steel needle component 67 to move to the position of the washing device 69; subsequently, by controlling the moving component 66 to drive the steel needle component 67, the steel needle component 67 is inserted into the washing device 69; inject cleaning liquid into the washing device 69 to perform the cleaning operation of the steel needle component 67. During this process, the magnetic component 61 remains stationary and does not perform any movement;

[0106] Following the specified operation process, the subsequent reagent addition step is performed by the second robotic arm module 5;

[0107] Subsequently, the magnetic component 61 descends to the initial position, and the central control system will control the eccentric oscillation module to start working to perform the vortex mixing operation of the flow tube.

[0108] If subsequent constant temperature and light-shielded incubation and cleaning are still required, repeat the above relevant operations; when the oscillation mixing and light-shielded incubation operations stop and end, take out the flow tube from the experimental incubation device 6 for subsequent flow cytometry detection operations.

[0109] The above are only the preferred embodiments of the present utility model and are not intended to limit the present utility model. For those skilled in the art, the present utility model can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model are all included within the protection scope of the present utility model.

Claims

1. A multi-mode flow lysis and elution instrument pretreatment system, characterized in that: It includes a central control system, an operating table, and a first mechanical arm module arranged above the operating table, a cover opening auxiliary module, a pipette tip storage device, a test tube storage device, a second mechanical arm module, an experimental incubation device, and a reagent storage device; The first robotic arm module and the second robotic arm module are both arranged above the cover opening auxiliary module, the pipette tip storage device, the test tube storage device, the experimental incubation device and the reagent storage device; the first robotic arm module, the cover opening auxiliary module, the second robotic arm module and the experimental incubation device are all electrically connected to the central control system; The first robot module and the second robot module are both capable of moving in the X, Y and Z directions; The experimental incubation device includes a magnetic adsorption unit, a constant temperature incubation unit and a cleaning head module. The magnetic adsorption unit is arranged below the constant temperature incubation unit. An incubation cabin for accommodating flow tubes is arranged inside the constant temperature incubation unit. The cleaning head module is arranged above the incubation cabin.

2. The multi-mode flow lysis and elution instrument pretreatment system according to claim 1, characterized in that: The magnetic adsorption unit comprises a magnetic component, a magnetic component slide rail and a magnetic component driving device, wherein the magnetic component driving device is connected to one side of the magnetic component; the magnetic component is slidably connected to the magnetic component slide rail.

3. The multi-mode flow lysis and elution instrument pretreatment system according to claim 2, characterized in that: The magnetic component comprises a plate body and magnetic plates. A plurality of magnetic plates are arranged in parallel above the plate body, and through holes are arranged between the magnetic plates.

4. The multi-mode flow lysis and elution instrument pretreatment system according to any one of claims 1 to 3, characterized in that: The constant temperature incubation unit comprises relatively arranged temperature increasing components, an incubation chamber is formed between the temperature increasing components, and a flow tube rack is arranged inside the incubation chamber.

5. The multi-mode flow lysis and elution instrument pretreatment system according to any one of claims 1 to 3, characterized in that: The cleaning head module comprises a moving component and a steel needle component. The steel needle component is arranged on one side of the moving component. A slide rail is arranged on the experimental incubation device, and the slide rail is slidably connected to the moving component.

6. The multi-mode flow lysis and elution instrument pretreatment system according to any one of claims 1 to 3, characterized in that: A washing device is arranged on one side of the constant temperature incubation unit, and a washing cavity is arranged inside the washing device.

7. The multi-mode flow lysis and elution instrument pretreatment system according to any one of claims 1 to 3, characterized in that: The experimental incubation device comprises a sample loading window, which is arranged above the incubation chamber.

8. The multi-mode flow lysis and elution instrument pretreatment system according to any one of claims 1 to 3, characterized in that: A cover plate is arranged at the sample loading window.

9. The multi-mode flow lysis and elution instrument pretreatment system according to any one of claims 1 to 3, characterized in that: The experimental incubation device comprises an eccentric shaking module.

10. The multi-mode flow lysis and elution instrument pretreatment system according to any one of claims 1 to 3, characterized in that: The test tube storage device comprises a tube rack, a storage table and an oscillating mechanism. A plurality of test tubes loaded with samples to be tested are arranged inside the tube rack, the tube rack is arranged above the storage table, and the oscillating mechanism is connected below the storage table.

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